ES2053978T3 - Enzima alfa-amidante en el c-terminal y procedimiento para su produccion. - Google Patents

Enzima alfa-amidante en el c-terminal y procedimiento para su produccion.

Info

Publication number
ES2053978T3
ES2053978T3 ES89302434T ES89302434T ES2053978T3 ES 2053978 T3 ES2053978 T3 ES 2053978T3 ES 89302434 T ES89302434 T ES 89302434T ES 89302434 T ES89302434 T ES 89302434T ES 2053978 T3 ES2053978 T3 ES 2053978T3
Authority
ES
Spain
Prior art keywords
peptide
enzyme
alpha
terminal
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89302434T
Other languages
English (en)
Inventor
Hisayuki Matsuo
Kensaku Mizuno
Masayasu Kojima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2053978T3 publication Critical patent/ES2053978T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/17—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced ascorbate as one donor, and incorporation of one atom of oxygen (1.14.17)
    • C12Y114/17003—Peptidylglycine monooxygenase (1.14.17.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

UNA ENZIMA ALFA - AMIDANTE EN C - TERMINAL DE ORIGEN PORCINO CON LAS SIGUIENTES PROPIEDADES: 1) ESPECIFICIDAD DE SUSTRATO Y ACCION: ACTUACION SOBRE UN PEPTIDO O PROTEINA REPRESENTADO POR LA FORMULA X - R - GLY DONDE GLY ES YM RESIDUO DE GLICINA EN C - TERMINAL, R ES UN RESIDUO DE AMINOACIDO PARA SER ALFA - AMIDADO Y X ES UNA PORCION RTEMANENTE DEL PEPTIDO O PROTEINA PARA CONVERTIRLO A UN PEPTIDO O PROTEINA REPRESENTADO POR LA FORMULA X - R - NH2 DONDE R - NH2 REPRESENTA UN RESIDUO AMINOACIDO ALFA - AMIDADO EN C - TERMINAL Y X ES UNA PORCION RESIDUAL DEL PEPTIDO O PROTEINA; (2) OPTIMO PH : 6.5 A 8.5; (3) PESO MOLECULAR, APROXIMADAMENTE 92000 SEGUN SE DETERMINA POR ELECTROLISIS SOBRE GEL DE POLIACRILAMIDA - SDS; Y (4) CONTIENE EL SIGUIENTE FRAGMENTO PEPTIDO: GLU - ALA - PRO - LEN - LEN - LLEN LEN - GLY...; Y UN PROCESO PARA LA PRODUCCION DE ENZIMA ALFA AMIDANTE EN C - TERMINAL COMPRENDIENDO LA ETAPA DE EXTRACCION Y PURIFICACION DE LA ENZIMA DE ATRIUM CORDIS PORCINO QUE EXHIBE LA ACTIVIDAD ENZIMATICA.
ES89302434T 1988-03-14 1989-03-13 Enzima alfa-amidante en el c-terminal y procedimiento para su produccion. Expired - Lifetime ES2053978T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP63058187A JP2653820B2 (ja) 1988-03-14 1988-03-14 アミド化酵素及びその製造方法

Publications (1)

Publication Number Publication Date
ES2053978T3 true ES2053978T3 (es) 1994-08-01

Family

ID=13077015

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89302434T Expired - Lifetime ES2053978T3 (es) 1988-03-14 1989-03-13 Enzima alfa-amidante en el c-terminal y procedimiento para su produccion.

Country Status (8)

Country Link
US (1) US5360727A (es)
EP (1) EP0333399B1 (es)
JP (1) JP2653820B2 (es)
AT (1) ATE85077T1 (es)
AU (1) AU612006B2 (es)
CA (1) CA1337860C (es)
DE (1) DE68904578T2 (es)
ES (1) ES2053978T3 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE68917154T2 (de) * 1988-05-30 1994-12-22 Shiseido Co Ltd C-terminus-amidierungsenzym-zusammensetzung, verfahren zur herstellung und verwendung.
ES2079054T3 (es) * 1990-06-01 1996-01-01 Ciba Geigy Ag Peptidilhidroxiglicina n-c-liasa y adn codificante de la misma.
IT1250689B (it) * 1991-07-22 1995-04-21 Marco Gerna Analoghi dell'irudina e procedimento per la loro preparazione
US6507788B1 (en) 1999-02-25 2003-01-14 Société de Conseils de Recherches et D'Applications Scientifiques (S.C.R.A.S.) Rational selection of putative peptides from identified nucleotide, or peptide sequences, of unknown function
US20080212024A1 (en) * 2006-09-18 2008-09-04 Lai Shui T Customized contact lenses for reducing aberrations of the eye

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8314362D0 (en) * 1983-05-24 1983-06-29 Celltech Ltd Polypeptide and protein products
US4708934A (en) * 1984-09-27 1987-11-24 Unigene Laboratories, Inc. α-amidation enzyme
GB8523156D0 (en) * 1985-09-19 1985-10-23 Celltech Ltd Polypeptide production
JPH0775541B2 (ja) * 1986-06-07 1995-08-16 壽之 松尾 Ｃ末端アミド化酵素及びその製造方法
WO1990002815A1 (en) * 1988-09-13 1990-03-22 The General Hospital Corporation Isolation, purification, and characterization of the aminopeptidases: mas ii and mas iii
WO1990002813A1 (en) * 1988-09-13 1990-03-22 The General Hospital Corporation Isolation, purification, and characterization of the aminopeptidases: ap1 and ap122

Also Published As

Publication number Publication date
JP2653820B2 (ja) 1997-09-17
JPH01231888A (ja) 1989-09-18
DE68904578D1 (de) 1993-03-11
US5360727A (en) 1994-11-01
AU612006B2 (en) 1991-06-27
ATE85077T1 (de) 1993-02-15
CA1337860C (en) 1996-01-02
AU3112889A (en) 1989-09-14
EP0333399A2 (en) 1989-09-20
EP0333399A3 (en) 1990-09-05
EP0333399B1 (en) 1993-01-27
DE68904578T2 (de) 1993-06-09

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