ES2050068A1 - Procedimiento para la purificacion de dos isoenzimas lipasa de candida rugosa. - Google Patents

Procedimiento para la purificacion de dos isoenzimas lipasa de candida rugosa.

Info

Publication number
ES2050068A1
ES2050068A1 ES09201378A ES9201378A ES2050068A1 ES 2050068 A1 ES2050068 A1 ES 2050068A1 ES 09201378 A ES09201378 A ES 09201378A ES 9201378 A ES9201378 A ES 9201378A ES 2050068 A1 ES2050068 A1 ES 2050068A1
Authority
ES
Spain
Prior art keywords
lipases
purification
lipase
present
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09201378A
Other languages
English (en)
Other versions
ES2050068B1 (es
Inventor
Rodriguez M Luisa Rua
Olmo Antonio Ballesteros
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9201378A priority Critical patent/ES2050068B1/es
Priority to PCT/ES1993/000058 priority patent/WO1994001542A1/es
Publication of ES2050068A1 publication Critical patent/ES2050068A1/es
Application granted granted Critical
Publication of ES2050068B1 publication Critical patent/ES2050068B1/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEDIMIENTO PARA LA PURIFICACION DE DOS ISOENZIMAS LIPASA DE CANDIDA RUGOSA. PROCEDIMIENTO PARA LA PURIFICACION DE DOS LIPASAS EXTRACELULARES PRESENTES EN LIPASA BRUTA COMERCIAL DE LA LEVADURA CANDIDA RUGOSA. EL METODO CONSTA DE UN SOLO PASO DE CROMATOGRAFIA HIDROFOBICA EN MATRIZ DE AGAROSA. CONDICIONES DE ELUCION MUY DIFERENTES PERMITEN LA COMPLETA SEPARACION Y PURIFICACION DE DOS ENZIMAS CON ACTIVIDAD LIPASICA (LIPASAS A Y B) PRESENTES EN EL EXTRACTO COMERCIAL DE PARTIDA. PARA ELLO SE UTILIZA UN TAMPON CONCENTRADO DE PH 6 A 8, CON EL QUE SE ELUYEN DE LA COLUMNA GRAN PARTE DE LOS CONTAMINANTES PRESENTES EN EL EXTRACTO; LUEGO CON LA MISMA SOLUCION TAMPON MAS DILUIDA SE ARRASTRA LA LIPASA B. POSTERIORMENTE, CON UN DI O POLIALCOHOL DISUELTO EN EL TAMPON DILUIDO SE ELUYE LA LIPASA A, DE CUYA SOLUCION, MEDIANTE CONCENTRACION POR ULTRAFILTRACION Y CROMATOGRAFIA DE EXCLUSION MOLECULAR CON DEXTRANO, SE ELIMINA EL ALCOHOL. SE APLICA INDUSTRIALMENTE EN LA PURIFICACION DE ENZIMAS.
ES9201378A 1992-07-03 1992-07-03 Procedimiento para la purificacion de dos isoenzimas lipasa de candida rugosa. Expired - Lifetime ES2050068B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES9201378A ES2050068B1 (es) 1992-07-03 1992-07-03 Procedimiento para la purificacion de dos isoenzimas lipasa de candida rugosa.
PCT/ES1993/000058 WO1994001542A1 (es) 1992-07-03 1993-07-02 PROCEDIMIENTO PARA LA PURIFICACION DE DOS ISOENZIMAS LIPASA DE $i(CANDIDA RUGOSA)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9201378A ES2050068B1 (es) 1992-07-03 1992-07-03 Procedimiento para la purificacion de dos isoenzimas lipasa de candida rugosa.

Publications (2)

Publication Number Publication Date
ES2050068A1 true ES2050068A1 (es) 1994-05-01
ES2050068B1 ES2050068B1 (es) 1994-12-16

Family

ID=8277528

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9201378A Expired - Lifetime ES2050068B1 (es) 1992-07-03 1992-07-03 Procedimiento para la purificacion de dos isoenzimas lipasa de candida rugosa.

Country Status (2)

Country Link
ES (1) ES2050068B1 (es)
WO (1) WO1994001542A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1012301A1 (en) * 1997-09-16 2000-06-28 Unilever N.V. TOTAL SYNTHESIS AND FUNCTIONAL OVEREXPRESSION OF A $i(CANDIDA RUGOSA) LIP1 GENE CODING FOR A MAJOR INDUSTRIAL LIPASE
GB9907082D0 (en) * 1999-03-26 1999-05-19 Chirotech Technology Ltd The preparation of carboxylic acid derivatives
AU2012256449B2 (en) * 2011-05-18 2015-04-09 Swedish Orphan Biovitrum Ab (Publ) Low pH protein purification process

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015146A1 (en) * 1989-06-05 1990-12-13 Rhone-Poulenc Inc. Enzymes and their use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015146A1 (en) * 1989-06-05 1990-12-13 Rhone-Poulenc Inc. Enzymes and their use

Also Published As

Publication number Publication date
ES2050068B1 (es) 1994-12-16
WO1994001542A1 (es) 1994-01-20

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