ES2037600A1 - "procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph) - Google Patents
"procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph)Info
- Publication number
- ES2037600A1 ES2037600A1 ES919101968A ES9101968A ES2037600A1 ES 2037600 A1 ES2037600 A1 ES 2037600A1 ES 919101968 A ES919101968 A ES 919101968A ES 9101968 A ES9101968 A ES 9101968A ES 2037600 A1 ES2037600 A1 ES 2037600A1
- Authority
- ES
- Spain
- Prior art keywords
- cell
- nadp
- nadph
- contg
- membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
PROCEDIMIENTO PARA LA REGENERACION FOTOQUIMICA DE DINUCLEOTIDO DE NICOTINAMIDA Y DE ADENINA FOSFATO REDUCIDO (NADPH). EL SISTEMA CONSTA COMO ELEMENTO CENTRAL DE UNA CELULA DE ULTRAFILTRACION (A) EQUIPADA CON UNA MEMBRANA QUE PERMITE EL PASO DE MOLECULAS DE PESO MOLECULAR INFERIOR A 5.000 DALTONS, Y QUE CONSTITUYE EL BIORREACTOR DONDE SE REALIZA LA CONVERSION DEL NADPBS+ EN NADPH. EN EL INTERIOR DE LA CELULA SE ENCUENTRAN INMOVILIZADAS LA ENZIMA FNR Y LA PROTEINA TRANSPORTADORA DE ELECTRONES FLAVODOXINA, QUE DEBIDO A SU PESO MOLECULAR NO PUEDEN ATRAVESAR LA MEMBRANA. LA MEZCLA DE REACTIVOS, QUE CONTIENE PROFLAVINA COMO FOTOSENSIBILIZADOR, EDTA COMO SUSTRATO SACRIFICIAL Y NADPBS+, SE HALLA EN UN DEPOSITO (B) Y SE INTRODUCE EN LA CELULA MEDIANTE PRESION. SE ILUMINA LA CELULA CON UNA LAMPARA HALOGENA (C) EQUIPADA CON UN FILTRO AZUL (F). EL NADPBS+ ES REDUCIDO A NADPH Y SE RECOGEN LAS FRACCIONES DE SALIDAEN EL COLECTOR (E).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9101968A ES2037600B1 (es) | 1991-09-02 | 1991-09-02 | "procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph) |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9101968A ES2037600B1 (es) | 1991-09-02 | 1991-09-02 | "procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph) |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2037600A1 true ES2037600A1 (es) | 1993-06-16 |
ES2037600B1 ES2037600B1 (es) | 1994-02-01 |
Family
ID=8273416
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9101968A Expired - Fee Related ES2037600B1 (es) | 1991-09-02 | 1991-09-02 | "procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph) |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2037600B1 (es) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2346449A1 (fr) * | 1976-04-01 | 1977-10-28 | Corning Glass Works | Reacteur pour microbes stabilises ayant une activite photometabolique |
US4352885A (en) * | 1980-05-09 | 1982-10-05 | Wisconsin Alumni Research Foundation | Preparation of a novel NADP linked alcohol-aldehyde/ketone oxidoreductase from thermophilic anaerobic bacteria for analytical and commercial use |
-
1991
- 1991-09-02 ES ES9101968A patent/ES2037600B1/es not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2346449A1 (fr) * | 1976-04-01 | 1977-10-28 | Corning Glass Works | Reacteur pour microbes stabilises ayant une activite photometabolique |
US4352885A (en) * | 1980-05-09 | 1982-10-05 | Wisconsin Alumni Research Foundation | Preparation of a novel NADP linked alcohol-aldehyde/ketone oxidoreductase from thermophilic anaerobic bacteria for analytical and commercial use |
Non-Patent Citations (1)
Title |
---|
ALBERT L. LEHNINGER "Bioquímica Lehninger" Segunda edición *Páginas 618-619* * |
Also Published As
Publication number | Publication date |
---|---|
ES2037600B1 (es) | 1994-02-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD2A | Announcement of lapse in spain |
Effective date: 20111209 |