ES2037600A1 - "procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph) - Google Patents

"procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph)

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Publication number
ES2037600A1
ES2037600A1 ES919101968A ES9101968A ES2037600A1 ES 2037600 A1 ES2037600 A1 ES 2037600A1 ES 919101968 A ES919101968 A ES 919101968A ES 9101968 A ES9101968 A ES 9101968A ES 2037600 A1 ES2037600 A1 ES 2037600A1
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Spain
Prior art keywords
cell
nadp
nadph
contg
membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES919101968A
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English (en)
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ES2037600B1 (es
Inventor
Dabad Jose Javier Pueyo
Calera Carlos Gomez-Moreno
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Universidad de Zaragoza
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Universidad de Zaragoza
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Application filed by Universidad de Zaragoza filed Critical Universidad de Zaragoza
Priority to ES9101968A priority Critical patent/ES2037600B1/es
Publication of ES2037600A1 publication Critical patent/ES2037600A1/es
Application granted granted Critical
Publication of ES2037600B1 publication Critical patent/ES2037600B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PROCEDIMIENTO PARA LA REGENERACION FOTOQUIMICA DE DINUCLEOTIDO DE NICOTINAMIDA Y DE ADENINA FOSFATO REDUCIDO (NADPH). EL SISTEMA CONSTA COMO ELEMENTO CENTRAL DE UNA CELULA DE ULTRAFILTRACION (A) EQUIPADA CON UNA MEMBRANA QUE PERMITE EL PASO DE MOLECULAS DE PESO MOLECULAR INFERIOR A 5.000 DALTONS, Y QUE CONSTITUYE EL BIORREACTOR DONDE SE REALIZA LA CONVERSION DEL NADPBS+ EN NADPH. EN EL INTERIOR DE LA CELULA SE ENCUENTRAN INMOVILIZADAS LA ENZIMA FNR Y LA PROTEINA TRANSPORTADORA DE ELECTRONES FLAVODOXINA, QUE DEBIDO A SU PESO MOLECULAR NO PUEDEN ATRAVESAR LA MEMBRANA. LA MEZCLA DE REACTIVOS, QUE CONTIENE PROFLAVINA COMO FOTOSENSIBILIZADOR, EDTA COMO SUSTRATO SACRIFICIAL Y NADPBS+, SE HALLA EN UN DEPOSITO (B) Y SE INTRODUCE EN LA CELULA MEDIANTE PRESION. SE ILUMINA LA CELULA CON UNA LAMPARA HALOGENA (C) EQUIPADA CON UN FILTRO AZUL (F). EL NADPBS+ ES REDUCIDO A NADPH Y SE RECOGEN LAS FRACCIONES DE SALIDAEN EL COLECTOR (E).
ES9101968A 1991-09-02 1991-09-02 "procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph) Expired - Fee Related ES2037600B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9101968A ES2037600B1 (es) 1991-09-02 1991-09-02 "procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9101968A ES2037600B1 (es) 1991-09-02 1991-09-02 "procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph)

Publications (2)

Publication Number Publication Date
ES2037600A1 true ES2037600A1 (es) 1993-06-16
ES2037600B1 ES2037600B1 (es) 1994-02-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES9101968A Expired - Fee Related ES2037600B1 (es) 1991-09-02 1991-09-02 "procedimiento para la regeneracion fotoquimica de dinucleotido de nicotinamida y de adenina fosfato reducido (nadph)

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ES (1) ES2037600B1 (es)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2346449A1 (fr) * 1976-04-01 1977-10-28 Corning Glass Works Reacteur pour microbes stabilises ayant une activite photometabolique
US4352885A (en) * 1980-05-09 1982-10-05 Wisconsin Alumni Research Foundation Preparation of a novel NADP linked alcohol-aldehyde/ketone oxidoreductase from thermophilic anaerobic bacteria for analytical and commercial use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2346449A1 (fr) * 1976-04-01 1977-10-28 Corning Glass Works Reacteur pour microbes stabilises ayant une activite photometabolique
US4352885A (en) * 1980-05-09 1982-10-05 Wisconsin Alumni Research Foundation Preparation of a novel NADP linked alcohol-aldehyde/ketone oxidoreductase from thermophilic anaerobic bacteria for analytical and commercial use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ALBERT L. LEHNINGER "Bioquímica Lehninger" Segunda edición *Páginas 618-619* *

Also Published As

Publication number Publication date
ES2037600B1 (es) 1994-02-01

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