ES2035017T3 - Procedimiento y soporte de ensayo para la determinacion de un analito. - Google Patents

Procedimiento y soporte de ensayo para la determinacion de un analito.

Info

Publication number
ES2035017T3
ES2035017T3 ES198787116936T ES87116936T ES2035017T3 ES 2035017 T3 ES2035017 T3 ES 2035017T3 ES 198787116936 T ES198787116936 T ES 198787116936T ES 87116936 T ES87116936 T ES 87116936T ES 2035017 T3 ES2035017 T3 ES 2035017T3
Authority
ES
Spain
Prior art keywords
binding
reaction
immobilised
carrier
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198787116936T
Other languages
English (en)
Inventor
Helmut Dr. Freitag
Hans-Erich Dr. Wilk
Anselm Dr. Rothe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2035017T3 publication Critical patent/ES2035017T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/525—Multi-layer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/823—Immunogenic carrier or carrier per se

Abstract

PROCEDIMIENTO Y SOPORTE DE COMPROBACION PARA DETERMINACION DE UN ANALISIS DE UNA PRUEBA, ESPECIALMENTE UN LIQUIDO. SE UTILIZAN DOS BIOMATERIALES (COMBINACIONES) QUE PUEDEN COMBINAR UNA CON OTRA ESPECIFICAMENTE DE LAS QUE UNA COMBINACION SE MARCA ENZIMATICAMENTE Y NO SE FIJA AL SOPORTE Y LA OTRA SE FIJA AL SOPORTE. EL PROCEDIMIENTO CONTIENE UN PASO DE REACCION EN QUE LAS COMBINACIONES SE INCUBAN UNAS CON OTRAS DE MODO QUE ENTRE ELLAS SE DESARROLLA UNA REACCION DE COMBINACION ESPECIFICA, CON LO QUE SEGUN EL DESARROLLO DE LA REACCION DE COMBINACION ESPECIFICA LA CANTIDAD DE LA COMBINACION MARCADA ENZIMATICA QUE NO SE HA UNIDO A LA COMBINACION FIJADA AL SOPORTE ES UNA MEDIDA PARA LA CONCENTRACION DEL ANALISIS. ESTA CANTIDAD SE DETERMINA DURANTE LA REACCION DE COMBINACION ESPECIFICA A LA VEZ CON UN SUSTRATO DE LA ENZIMA DE MARCAJE NO FIJADO QUE NO PRODUCE NINGUNA SEÑAL DE COMPROBACION Y SE INCUBA CON UN SUSTRATO DE COMPROBACION, CON LO QUE ES SUSTRATO QUE NO PRODUCE UNA SEÑAL DE COMPROBACION SE ELIGE DE MODO QUE EN LA INCUBACION SIMULTANEA DE LA COMBINACION MARCADA ENZIMATICA LIBRE CON AMBOS SUSTRATOS LA REACCION CATALIZADORA ENZIMATICA DEL SUSTRATO QUE PRODUCE SEÑAL DE COMPROBACION SE RETRASA EL TIEMPO NECESARIO HASTA QUE SE PRODUCE EN LO ESENCIAL LA REACCION DE COMBINACION ESPECIFICA ENTRE LAS COMBINACIONES.
ES198787116936T 1986-11-26 1987-11-17 Procedimiento y soporte de ensayo para la determinacion de un analito. Expired - Lifetime ES2035017T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19863640318 DE3640318A1 (de) 1986-11-26 1986-11-26 Verfahren und testtraeger zur bestimmung eines analyten

Publications (1)

Publication Number Publication Date
ES2035017T3 true ES2035017T3 (es) 1993-04-16

Family

ID=6314766

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198787116936T Expired - Lifetime ES2035017T3 (es) 1986-11-26 1987-11-17 Procedimiento y soporte de ensayo para la determinacion de un analito.

Country Status (9)

Country Link
US (1) US5037736A (es)
EP (1) EP0268978B1 (es)
JP (1) JPS63142267A (es)
AT (1) ATE80463T1 (es)
CA (1) CA1293189C (es)
DE (2) DE3640318A1 (es)
DK (1) DK608387A (es)
ES (1) ES2035017T3 (es)
FI (1) FI875210A (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1303983C (en) * 1987-03-27 1992-06-23 Robert W. Rosenstein Solid phase assay
USRE38430E1 (en) * 1987-03-27 2004-02-17 Becton, Dickinson And Company Solid phase chromatographic immunoassay
DE4015378A1 (de) * 1990-05-14 1991-11-21 Boehringer Mannheim Gmbh Testtraeger fuer die analytische bestimmung eines bestandteils einer probenfluessigkeit
CA2027434C (en) * 1990-10-12 1999-01-05 George Jackowski Diagnostic kit for diagnosing and distinguishing chest pain in early onset thereof
US5271895A (en) * 1991-02-27 1993-12-21 Boehringer Mannheim Corporation Test strip
ATE162628T1 (de) * 1991-02-27 1998-02-15 Boehringer Mannheim Corp Verbesserter teststreifen
DE4201831A1 (de) * 1992-01-24 1993-07-29 Metallgesellschaft Ag Verfahren zur entsorgung von reststoffen, die fluor- und zyanidhaltige verbindungen enthalten
EP1417491A4 (en) * 2001-07-18 2004-08-11 Siliang Zhou TEST STRIP FOR SIDE FLOW ASSAY OF A SAMPLE CONTAINING WHOLE CELLS
JP2006518217A (ja) * 2003-02-11 2006-08-10 ケムセンシング，インコーポレーテッド 被検化合物を検出するための方法および装置
US8003407B2 (en) * 2004-07-29 2011-08-23 Relia Diagnostic Systems, Llc Lateral flow system and assay
NZ555339A (en) * 2004-11-24 2010-03-26 Techlab Inc Device and method for detection of analytes
JP2010117213A (ja) * 2008-11-12 2010-05-27 Dic Plastics Inc 酵素免疫測定器具及び酵素免疫測定方法
JP5793075B2 (ja) 2009-04-09 2015-10-14 アークレイ株式会社 検体分析用具、検体分析用具の製造方法および展開部材の液体浸透性低下抑制方法
JP2011095164A (ja) * 2009-10-30 2011-05-12 Tdk Corp 分析チップ及び分析チップの使用方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3964871A (en) * 1974-12-18 1976-06-22 Becton, Dickinson And Company Method and device for detecting glucose
JPS57136163A (en) * 1981-02-18 1982-08-23 Eisai Co Ltd Method for assaying material by enzyme immunoassay
US4446232A (en) * 1981-10-13 1984-05-01 Liotta Lance A Enzyme immunoassay with two-zoned device having bound antigens
US4510239A (en) * 1982-08-09 1985-04-09 Beckman Instruments, Inc. Double antibody immunoassays of enzymes such as prostatic acid phosphatase
DE3234478A1 (de) * 1982-09-17 1984-03-22 Boehringer Mannheim Gmbh, 6800 Mannheim Reagenz und verfahren zur bestimmung von (gamma)-glutamyltransferase
DE3247608A1 (de) * 1982-12-23 1984-07-05 Boehringer Mannheim Gmbh, 6800 Mannheim Teststreifen
DE3247894A1 (de) * 1982-12-24 1984-06-28 Merck Patent Gmbh, 6100 Darmstadt Testsystem und verfahren zur bestimmung von nad(p)h
US4513088A (en) * 1983-03-30 1985-04-23 The Board Of Trustees Of The Leland Stanford Junior University Assay for monoclonal antibody against surface Ig of a human B cell tumor
US4828981A (en) * 1983-08-24 1989-05-09 Synbiotics Corporation Immunoassays for determining Dirofilaria immitis infection using antiidiotype monoclonal antibody reagents
US4804625A (en) * 1984-09-27 1989-02-14 Amoco Corporation Assay procedures
JPH0656383B2 (ja) * 1985-02-22 1994-07-27 オリンパス光学工業株式会社 酵素免疫学的自動分析装置
US4837395A (en) * 1985-05-10 1989-06-06 Syntex (U.S.A.) Inc. Single step heterogeneous assay
US4661445A (en) * 1985-05-24 1987-04-28 Saxinger W Carl Competitive ELISA for the detection of HTLV-III antibodies
US4806312A (en) * 1985-08-28 1989-02-21 Miles Inc. Multizone analytical element having detectable signal concentrating zone
JPS6319559A (ja) * 1986-07-11 1988-01-27 Fuji Photo Film Co Ltd 免疫分析方法

Also Published As

Publication number Publication date
DE3640318A1 (de) 1988-06-09
ATE80463T1 (de) 1992-09-15
CA1293189C (en) 1991-12-17
EP0268978A3 (en) 1990-09-05
FI875210A0 (fi) 1987-11-25
EP0268978B1 (de) 1992-09-09
DK608387D0 (da) 1987-11-19
EP0268978A2 (de) 1988-06-01
DK608387A (da) 1988-05-27
FI875210A (fi) 1988-05-27
US5037736A (en) 1991-08-06
DE3781643D1 (de) 1992-10-15
JPS63142267A (ja) 1988-06-14

Similar Documents

Publication Publication Date Title
ES2022166B3 (es) Ensayo de enlace especifico heterogeneo que emplea un reactivo enlazante agregable.
ES2035017T3 (es) Procedimiento y soporte de ensayo para la determinacion de un analito.
DE19781511B4 (de) Vorrichtungen und Verfahren zum Testen des Vorhandenseins oder der Konzentration eines Analyten in einer Fluidprobe
MX9606509A (es) Colorante de copulacion estable para determinacion fotometrica de analitos.
DE3365104D1 (en) Enzyme/immunofluorescent assay for autoantibodies
WO2003014740A1 (fr) Biocapteurs et procede de mesure
YU11500A (sh) Metod i uredjaji za brzu analizu analita u biološkim uzorcima
SE9604575D0 (sv) Method and system for analyte determination
PL309211A1 (en) Heterogenous specific bond test used for detection or content determination of a given substance in a sample analysed, method of reducing non-specific bonding in pseudohomogenous tests and testing set for performing immunological test and those employing genetic probe
ATE101722T1 (de) Qualitativer enzymtest zur visuellen auswertung.
AU547916B2 (en) Wipe-off test device
DE69928507D1 (de) Festphasenverfahren und testsatz zur antigen- und antikörperprüfung in der blutgruppen-serologie
NO331074B1 (no) Analysemetode for bestemmelse av holo-Transcobalamin II i en cellefri prove i et kroppsfluid.
NO163586C (no) Immunologiske jodtyroninanalyser hvor hms brukes som tbp-blokkerende middel.
AU611218B2 (en) Method for self-performing enzyme kinetics
EP0189925A3 (en) Self-timed immunoassay device
DK1057021T3 (da) Fremgangsmåde til måling af totalanalyt
DE3785567D1 (de) Immunologischer testkit.
EP0368208A3 (en) Immunoassay method and test kit therefor
DK0497870T3 (da) Enzymassay og assaysæt til måling af cellulær aktivering
DE69424098T2 (de) Immunoassay-Verfahren
KR900016757A (ko) 유체 시료에서 변성 lgA₁의 검출
AU1705801A (en) Dynamic determination of analytes
PT1029075E (pt) Deteccao de sacarose por ensaio de imunossorcao enzimatica

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 268978

Country of ref document: ES