ES2032862T3 - Una secuencia nucleotida capaz de inducir altos niveles de translacion de un gen heterologo en bacillus subtilis y escherichia coli. - Google Patents

Una secuencia nucleotida capaz de inducir altos niveles de translacion de un gen heterologo en bacillus subtilis y escherichia coli.

Info

Publication number
ES2032862T3
ES2032862T3 ES198787830141T ES87830141T ES2032862T3 ES 2032862 T3 ES2032862 T3 ES 2032862T3 ES 198787830141 T ES198787830141 T ES 198787830141T ES 87830141 T ES87830141 T ES 87830141T ES 2032862 T3 ES2032862 T3 ES 2032862T3
Authority
ES
Spain
Prior art keywords
gene
heterologic
translation
nucleotide sequence
escherichia coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198787830141T
Other languages
English (en)
Inventor
Guido Grandi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eni Tecnologie SpA
Original Assignee
Eniricerche SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche SpA filed Critical Eniricerche SpA
Application granted granted Critical
Publication of ES2032862T3 publication Critical patent/ES2032862T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

SECUENCIAS DE NUCLEOTIDOS CAPACES DE INDUCIR ALTOS NIVELES DE TRADUCCION DE UN GEN HETEROLOGO EN BACILUS SUBTILIS Y ESCHERICHIA COLI Y UN METODO PARA PREPARAR PROTEINAS HETEROLOGAS QUE COMPRENDE EL CULTIVO, EN UN MEDIO ADECUADO, DE CELULAS DE BACILLUS SUBTILIS Y ESCHERICHIA COLI TRANSFORMADAS POR PLASMIDOS HIBRIDOS, EN LAS QUE LA SECUENCIA DE NUCLEOTIDOS ESTA SITUADA ENTRE EL LUGAR DE RECONOCIMIENTO DE LOS RIBOSOMAS Y EL TRIPLETE INICIADOR (ATG) DEL GEN ESTRUCTURAL DE CODIFICACION PARA LAS PROTEINAS DESEADAS. LEYENDA DE LA FIGURA 1: 1.-PROMOTOR 2.-TERMINADOR.
ES198787830141T 1986-05-07 1987-04-13 Una secuencia nucleotida capaz de inducir altos niveles de translacion de un gen heterologo en bacillus subtilis y escherichia coli. Expired - Lifetime ES2032862T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT20344/86A IT1221765B (it) 1986-05-07 1986-05-07 Sequenza nucleotidica capace di indurre alti livelli di traduzione di un gene eterologo in bacillus subtilis ed escherichia coli

Publications (1)

Publication Number Publication Date
ES2032862T3 true ES2032862T3 (es) 1993-03-01

Family

ID=11165894

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198787830141T Expired - Lifetime ES2032862T3 (es) 1986-05-07 1987-04-13 Una secuencia nucleotida capaz de inducir altos niveles de translacion de un gen heterologo en bacillus subtilis y escherichia coli.

Country Status (8)

Country Link
US (1) US4987069A (es)
EP (1) EP0245217B1 (es)
JP (1) JP2588402B2 (es)
AT (1) ATE73496T1 (es)
DE (1) DE3777238D1 (es)
ES (1) ES2032862T3 (es)
GR (1) GR3004560T3 (es)
IT (1) IT1221765B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2636644B1 (fr) * 1988-08-24 1990-12-28 Sanofi Sa Sequence d'adn participant a la regulation de l'expression d'une sequence d'adn codant pour un precurseur d'un polypeptide, vecteurs d'expression et procede de production periplasmique du polypeptide
US5279947A (en) * 1988-08-24 1994-01-18 Sanofi DNA sequence participating in the regulation of the expression of a DNA sequence coding for a precursor of a polypeptide, expression vectors and process for the periplasmic production of the polypeptide

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4529695A (en) * 1981-06-18 1985-07-16 Wisconsin Alumni Research Foundation Recombinant cloning vector, host for the vector, and method for using same
IT1156317B (it) * 1982-09-08 1987-02-04 Anic Spa Vettori plasmidici ad espressione in bacillus subtilis e procedimento per la loro preparazione
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド

Also Published As

Publication number Publication date
JPS63219383A (ja) 1988-09-13
JP2588402B2 (ja) 1997-03-05
EP0245217A2 (en) 1987-11-11
IT8620344A0 (it) 1986-05-07
EP0245217A3 (en) 1988-11-09
ATE73496T1 (de) 1992-03-15
EP0245217B1 (en) 1992-03-11
US4987069A (en) 1991-01-22
GR3004560T3 (es) 1993-04-28
IT1221765B (it) 1990-07-12
DE3777238D1 (de) 1992-04-16

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