ES2023631T3 - Ensayo de fagocitosis. - Google Patents

Ensayo de fagocitosis.

Info

Publication number
ES2023631T3
ES2023631T3 ES90125282T ES90125282T ES2023631T3 ES 2023631 T3 ES2023631 T3 ES 2023631T3 ES 90125282 T ES90125282 T ES 90125282T ES 90125282 T ES90125282 T ES 90125282T ES 2023631 T3 ES2023631 T3 ES 2023631T3
Authority
ES
Spain
Prior art keywords
phagocytosis
added
fluorescence
phagocytosis test
leukocytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90125282T
Other languages
English (en)
Other versions
ES2023631A4 (es
Inventor
Thomas C Dr Nebe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ORPEGEN MED MOLEKULARBIOFORSCH
Original Assignee
ORPEGEN MED MOLEKULARBIOFORSCH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ORPEGEN MED MOLEKULARBIOFORSCH filed Critical ORPEGEN MED MOLEKULARBIOFORSCH
Publication of ES2023631A4 publication Critical patent/ES2023631A4/es
Application granted granted Critical
Publication of ES2023631T3 publication Critical patent/ES2023631T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/10—DNA staining
    • C12Q2304/13—Propidium

Abstract

LA INVENCION TRATA DE UN PROCEDIMIENTO QUE SE EMPLEA PARA LA DETERMINACION DE LA ACTIVIDAD DE FAGOCITOSIS DE LOS LECUOCITOS Y CONSISTE EN UNA MUESTRA DE SANGRE CON UNA CANTIDAD DE BACTERIAS E. COLI MARCADAS CON FITC; INCUBAR LA MUESTRA MARCADA A UNA TEMPERATURA ENTRE 30 Y 45 C, DURANTE 5 A 60 MINUTOS; CONTRASTAR LA FAGOCITOSIS A TRAVES DEL ENFRIAMIENTO DE LA MUESTRA; AÑADIR A LA MUESTRA UNA SUBSTANCIA CAPAZ DE HINCHARSE; LISISAR LOS ERITROCITOS EXISTENTES EN LA SOLUCION DE LA MUESTRA ; AÑADIR A LA MUESTRA LISISADA COLORANTE DNA FLUORESCENTE Y ANALIZAR LA FLUORESCENCIA EN UN ZITOMETRO ADECUADO.
ES90125282T 1989-12-29 1990-12-21 Ensayo de fagocitosis. Expired - Lifetime ES2023631T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3943325 1989-12-29
DE4014393A DE4014393A1 (de) 1989-12-29 1990-05-04 Phagozytose-test

Publications (2)

Publication Number Publication Date
ES2023631A4 ES2023631A4 (es) 1992-02-01
ES2023631T3 true ES2023631T3 (es) 1995-08-16

Family

ID=25888629

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90125282T Expired - Lifetime ES2023631T3 (es) 1989-12-29 1990-12-21 Ensayo de fagocitosis.

Country Status (6)

Country Link
EP (1) EP0435226B1 (es)
AT (1) ATE123071T1 (es)
DE (2) DE4014393A1 (es)
DK (1) DK0435226T3 (es)
ES (1) ES2023631T3 (es)
GR (1) GR910300103T1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4117459C2 (de) * 1991-05-28 1994-09-22 Orpegen Med Molekularbioforsch BURST-Test
DE4334935C1 (de) * 1993-10-13 1995-03-09 Luciana Husfeld Verfahren zur Bestimmung der Phagozytose- und/oder Killingfähigkeit
FR2753536B1 (fr) * 1996-09-16 1998-11-20 Aetsrn Procede d'evaluation de la phagocytose de cellules cibles par des macrophages in vitro ; applications diagnostiques et application au controle de qualite de stocks de cellules
DE19950262C2 (de) * 1999-10-18 2001-09-13 Martin Foermer Verfahren zur Durchführung eines durchflusszytometrischen Phagozytosetests
JPWO2002101037A1 (ja) * 2001-05-31 2005-04-07 扶桑薬品工業株式会社 食細胞機能の評価方法およびその利用
DE10361243B4 (de) * 2003-12-22 2007-07-12 Universität Ulm Untersuchungsverfahren zur Phagozytose
CN108300754A (zh) * 2018-01-03 2018-07-20 王洵 一种检测细胞吞噬作用的荧光试剂盒及其制备方法

Also Published As

Publication number Publication date
DE4014393A1 (de) 1991-07-04
DE59009136D1 (de) 1995-06-29
DK0435226T3 (da) 1995-10-16
EP0435226A1 (de) 1991-07-03
GR910300103T1 (en) 1991-12-10
ES2023631A4 (es) 1992-02-01
ATE123071T1 (de) 1995-06-15
EP0435226B1 (de) 1995-05-24

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