ES2012728A6 - Analytical method for the selective determination of sialic acid-carrying glycolipid complexes. - Google Patents

Analytical method for the selective determination of sialic acid-carrying glycolipid complexes.

Info

Publication number
ES2012728A6
ES2012728A6 ES8901972A ES8901972A ES2012728A6 ES 2012728 A6 ES2012728 A6 ES 2012728A6 ES 8901972 A ES8901972 A ES 8901972A ES 8901972 A ES8901972 A ES 8901972A ES 2012728 A6 ES2012728 A6 ES 2012728A6
Authority
ES
Spain
Prior art keywords
sialic acid
carrying
water
selective determination
analytical method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES8901972A
Other languages
Spanish (es)
Inventor
Bela Szajani
Ferenc Pribek
Bela Schumann
Anna Gesztesi
Kinzcel
Iren Milovan
Jolan Babarczi
Edit Siklosi
Katalin Ivony
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Reanal Finomvegyszergyar Rt
Original Assignee
Reanal Finomvegyszergyar Rt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from HU219089A external-priority patent/HU208377B/en
Application filed by Reanal Finomvegyszergyar Rt filed Critical Reanal Finomvegyszergyar Rt
Publication of ES2012728A6 publication Critical patent/ES2012728A6/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose

Abstract

The invention relates to a method for the selective determination of sialic acid-carrying glycolipid complexes in blood, in which the serum is diluted with water, then extracted with a water-immiscible organic solvent or solvent mixture to remove neutral lipids, a lipoprotein fraction containing sialic acid-carrying glycolipids is precipitated from the aqueous phase, the precipitated fraction is redissolved, and the sialic acid content of the solution or the amount and quality of the sialic acid-carrying glycolipid is(are) determined by a method known per se. According to the invention a water-soluble salt of a monovalent, bivalent or trivalent metal other than alkali metal is applied as precipitating agent in an amount corresponding to a final metal ion concentration of 0.005-0.1 mole/l in the mixture, and precipitation is performed at a pH of 1.5 to 8.0.
ES8901972A 1989-01-23 1989-06-06 Analytical method for the selective determination of sialic acid-carrying glycolipid complexes. Expired - Fee Related ES2012728A6 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU24989 1989-01-23
HU219089A HU208377B (en) 1989-05-08 1989-05-08 Analytic method for selective detecting glycolipid complexes carrying sial acid

Publications (1)

Publication Number Publication Date
ES2012728A6 true ES2012728A6 (en) 1990-04-01

Family

ID=26317184

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8901972A Expired - Fee Related ES2012728A6 (en) 1989-01-23 1989-06-06 Analytical method for the selective determination of sialic acid-carrying glycolipid complexes.

Country Status (10)

Country Link
EP (1) EP0515355A1 (en)
JP (1) JPH02206763A (en)
KR (1) KR910700457A (en)
CN (1) CN1044537A (en)
AU (1) AU3735689A (en)
DK (1) DK226590D0 (en)
ES (1) ES2012728A6 (en)
FI (1) FI913518A0 (en)
NO (1) NO903877D0 (en)
WO (1) WO1990008317A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342567A (en) * 1981-07-06 1982-08-03 Sloan Kettering Institute For Cancer Research Sialic acid determination method

Also Published As

Publication number Publication date
WO1990008317A1 (en) 1990-07-26
FI913518A0 (en) 1991-07-23
KR910700457A (en) 1991-03-15
CN1044537A (en) 1990-08-08
AU3735689A (en) 1990-08-13
DK226590A (en) 1990-09-20
DK226590D0 (en) 1990-09-20
JPH02206763A (en) 1990-08-16
EP0515355A1 (en) 1992-12-02
NO903877L (en) 1990-09-05
NO903877D0 (en) 1990-09-05

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000301