KR910700457A - Method for Determination of Glycolipide Complex Carrying Sialic Acid - Google Patents

Method for Determination of Glycolipide Complex Carrying Sialic Acid

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Publication number
KR910700457A
KR910700457A KR1019900701970A KR900701970A KR910700457A KR 910700457 A KR910700457 A KR 910700457A KR 1019900701970 A KR1019900701970 A KR 1019900701970A KR 900701970 A KR900701970 A KR 900701970A KR 910700457 A KR910700457 A KR 910700457A
Authority
KR
South Korea
Prior art keywords
water
precipitant
precipitation
soluble
salt
Prior art date
Application number
KR1019900701970A
Other languages
Korean (ko)
Inventor
스자자니 벨라
프리벡 페렌크
슈만 벨라
니 쾨제기 안나 게체시 길레인
킨첼 에디트
밀로반 이렌
바바르치 졸란
니 파타이 시클로시 아띨레인
니 클라디바 카탈린 이보니 조세핀
Original Assignee
프리벡 페렌크
리날 피놈 베기스제르갸르
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from HU219089A external-priority patent/HU208377B/en
Application filed by 프리벡 페렌크, 리날 피놈 베기스제르갸르 filed Critical 프리벡 페렌크
Publication of KR910700457A publication Critical patent/KR910700457A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose

Abstract

내용 없음.No content.

Description

시알산을 운반하는 글리코리피드 복합체의 정량방법Method for Determination of Glycolipide Complex Carrying Sialic Acid

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (12)

혈액내의 글리코리피드 복합체를 운반하는 시알산에 있어서, 혈청을 물로 희석하고 물과 혼합되지 않는 유기 용매나 혼합용매로 추출하여 중성지질을 제거하고 시알산을 운반하는 글리코리피드를 함유하는 리포프로 테인분획을 수용액 상태로 침전시키고, 침전된 분획을 다시 용해시킨 후 상기 용액내에 있는 시알산의 양이나 시알산을 운반하는 글리코리피드의 양과 공지의 방법으로 정량하여서 되는 혈액, 특히 인간 혈액내에 있는 시알산을 운반하는 글리코리피드복합체를 선택적으로 정량하는 방법.In sialic acid carrying glycolipide complex in blood, lipoprotein fraction containing glycolipide carrying sialic acid is removed by diluting serum with water and extracting with organic solvent or mixed solvent which is not mixed with water. Is precipitated in aqueous solution, and the precipitated fraction is dissolved again, and the amount of sialic acid in the solution or the amount of glycolipides carrying sialic acid and sialic acid in the blood, in particular, quantified by a known method. A method of selectively quantifying a glycolipide complex to be carried. 제1항에 있어서, 침전제로서 수용성의 Fe2+염을 사용하며, pH 3.0 내지 3.5에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Fe 2+ salt is used as the precipitant, and the precipitation is carried out at pH 3.0 to 3.5. 제1항에 있어서, 침전제로서 수용성의 Fe3+염을 사용하며, pH 3.0 내지 5.0에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Fe 3+ salt is used as the precipitant, and the precipitation is carried out at pH 3.0 to 5.0. 제1항에 있어서, 침전제로서 수용성의 Co2+염을 사용하며, pH 4.0 내지 5.5에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Co 2+ salt is used as the precipitant, and the precipitation is carried out at pH 4.0 to 5.5. 제1항에 있어서, 침전제로서 수용성의 Ni2+염을 사용하며, pH 4.5 내지 5.5에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Ni 2+ salt is used as the precipitant, and the precipitation is carried out at pH 4.5 to 5.5. 제1항에 있어서, 침전제로서 수용성의 Cd2+염을 사용하며, pH 4.5 내지 6.0에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Cd 2+ salt is used as the precipitant, and the precipitation is carried out at pH 4.5 to 6.0. 제1항에 있어서, 침전제로서 수용성의 Zn2+염을 사용하며, pH 1.5 내지 5.0에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Zn 2+ salt is used as the precipitant, and the precipitation is carried out at pH 1.5 to 5.0. 제1항에 있어서, 침전제로서 수용성의 Ag+염을 사용하며, pH 6.0 내지 8.0에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Ag + salt is used as the precipitant, and the precipitation is carried out at pH 6.0 to 8.0. 제1항에 있어서, 침전제로서 수용성의 Hg2+염을 사용하며, pH 1.5 내지 3.0에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Hg 2+ salt is used as the precipitant, and the precipitation is carried out at pH 1.5 to 3.0. 제1항에 있어서, 침전제로서 수용성의 Cu2+염을 사용하며, pH 3.0내지 4.5에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Cu 2+ salt is used as the precipitant, and the precipitation is carried out at pH 3.0 to 4.5. 제1항에 있어서, 침전제로서 수용성의 Ca2+염을 사용하며, pH 4.5 내지 5.5에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Ca 2+ salt is used as the precipitant, and the precipitation is carried out at pH 4.5 to 5.5. 제1항에 있어서, 침전제로서 수용성의 Mg2+염을 사용하며, pH 5.5 내지 7.0에서 침전을 실시하여서 되는 것을 특징으로 하는 방법.The method according to claim 1, wherein a water-soluble Mg 2+ salt is used as the precipitant, and the precipitation is carried out at pH 5.5 to 7.0. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900701970A 1989-01-23 1989-05-12 Method for Determination of Glycolipide Complex Carrying Sialic Acid KR910700457A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
HU2190/89 1989-01-23
HU249/89 1989-01-23
HU24989 1989-01-23
HU219089A HU208377B (en) 1989-05-08 1989-05-08 Analytic method for selective detecting glycolipid complexes carrying sial acid
PCT/HU1989/000021 WO1990008317A1 (en) 1989-01-23 1989-05-12 Analytical method for the selective determination of sialic acid-carrying glycolipid complexes

Publications (1)

Publication Number Publication Date
KR910700457A true KR910700457A (en) 1991-03-15

Family

ID=26317184

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900701970A KR910700457A (en) 1989-01-23 1989-05-12 Method for Determination of Glycolipide Complex Carrying Sialic Acid

Country Status (10)

Country Link
EP (1) EP0515355A1 (en)
JP (1) JPH02206763A (en)
KR (1) KR910700457A (en)
CN (1) CN1044537A (en)
AU (1) AU3735689A (en)
DK (1) DK226590D0 (en)
ES (1) ES2012728A6 (en)
FI (1) FI913518A0 (en)
NO (1) NO903877D0 (en)
WO (1) WO1990008317A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342567A (en) * 1981-07-06 1982-08-03 Sloan Kettering Institute For Cancer Research Sialic acid determination method

Also Published As

Publication number Publication date
NO903877L (en) 1990-09-05
DK226590A (en) 1990-09-20
AU3735689A (en) 1990-08-13
FI913518A0 (en) 1991-07-23
JPH02206763A (en) 1990-08-16
DK226590D0 (en) 1990-09-20
EP0515355A1 (en) 1992-12-02
NO903877D0 (en) 1990-09-05
CN1044537A (en) 1990-08-08
ES2012728A6 (en) 1990-04-01
WO1990008317A1 (en) 1990-07-26

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