ES2013572A6 - Method for the determination of lipid-bound sialic acid content of blood - Google Patents

Method for the determination of lipid-bound sialic acid content of blood

Info

Publication number
ES2013572A6
ES2013572A6 ES8902167A ES8902167A ES2013572A6 ES 2013572 A6 ES2013572 A6 ES 2013572A6 ES 8902167 A ES8902167 A ES 8902167A ES 8902167 A ES8902167 A ES 8902167A ES 2013572 A6 ES2013572 A6 ES 2013572A6
Authority
ES
Spain
Prior art keywords
lipid
sialic acid
bound sialic
solution
acid content
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES8902167A
Other languages
Spanish (es)
Inventor
Bela Schumann
Tamas Pulay
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Reanal Finomvegyszergyar Rt
Original Assignee
Reanal Finomvegyszergyar Rt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reanal Finomvegyszergyar Rt filed Critical Reanal Finomvegyszergyar Rt
Publication of ES2013572A6 publication Critical patent/ES2013572A6/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57469—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving tumor associated glycolinkage, i.e. TAG
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a method for the determination of lipid-bound sialic acid content of blood, primarily of human blood, in which serum is diluted with water, then extracted with a water-immiscible organic solvent or solvent mixture to remove neutral lipids, a lipoprotein fraction containing lipid-bound sialic acid is precipitated from the aqueous phase, the precipitated lipoprotein fraction is redissolved, a mineral acid, a copper(II) salt and resorcine are added to the solution, and the lipid-bound sialic acid content of the serum is calculated on the basis of the colour reaction produced with resorcine. According to the invention a) dextrane with a molecular weight of 10,000 to 20,000 is applied as precipitating agent in a solution with a concentration of 15-35 mg/100 ml and precipitation is performed at a pH of 5.8 to 7.5, or b) polyethylene glycol with a molecular weight of 4,000 to 10,000 is applied as precipitating agent in a solution with a concentration of 15-55 g/100 ml and precipitation is performed at a pH of 9 to 11, or c) a water-soluble manganese(II) salt is applied as precipitating agent in a solution corresponding to a Mn<2+> ion concentration of 8.7-13 g/100 ml and precipitation is performed at a pH of 5.5 to 7.5.
ES8902167A 1989-02-24 1989-06-21 Method for the determination of lipid-bound sialic acid content of blood Expired - Fee Related ES2013572A6 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU89885A HU204131B (en) 1989-02-24 1989-02-24 Method for detecting sial acidcontent adherent to the lipid of the blood

Publications (1)

Publication Number Publication Date
ES2013572A6 true ES2013572A6 (en) 1990-05-01

Family

ID=10951876

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8902167A Expired - Fee Related ES2013572A6 (en) 1989-02-24 1989-06-21 Method for the determination of lipid-bound sialic acid content of blood

Country Status (7)

Country Link
JP (1) JPH02242160A (en)
KR (1) KR920700401A (en)
CN (1) CN1045185A (en)
AU (1) AU3697189A (en)
ES (1) ES2013572A6 (en)
HU (1) HU204131B (en)
WO (1) WO1990010225A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5462877A (en) * 1993-11-05 1995-10-31 Katopodis; Nonda Method for determining lipid bound sialic acid in plasma or serum
EP1002232A4 (en) * 1997-07-15 2004-05-26 Bioprobes Inc Determining alcohol intake using sialic acid/apo j
CA3125914A1 (en) * 2019-01-07 2020-07-16 1866402 Ontario Limited Blood separation and analysis device and methods

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342567A (en) * 1981-07-06 1982-08-03 Sloan Kettering Institute For Cancer Research Sialic acid determination method
JPH0775557B2 (en) * 1986-04-25 1995-08-16 富士レビオ株式会社 Method for measuring lipid-bound sialic acid

Also Published As

Publication number Publication date
AU3697189A (en) 1990-09-26
KR920700401A (en) 1992-02-19
HU204131B (en) 1991-11-28
JPH02242160A (en) 1990-09-26
CN1045185A (en) 1990-09-05
WO1990010225A1 (en) 1990-09-07
HUT53227A (en) 1990-09-28

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19990405