EP4701784A1 - Halbfestphase-extraktion von nukleinsäuren unter verwendung einer mischung aus quaternärem salz und kation unter alkalischen bedingungen - Google Patents

Halbfestphase-extraktion von nukleinsäuren unter verwendung einer mischung aus quaternärem salz und kation unter alkalischen bedingungen

Info

Publication number
EP4701784A1
EP4701784A1 EP24725406.3A EP24725406A EP4701784A1 EP 4701784 A1 EP4701784 A1 EP 4701784A1 EP 24725406 A EP24725406 A EP 24725406A EP 4701784 A1 EP4701784 A1 EP 4701784A1
Authority
EP
European Patent Office
Prior art keywords
nucleic acids
quats
buffer
sample
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24725406.3A
Other languages
English (en)
French (fr)
Inventor
Ludovic BARAULT
Max HAESENDONCKX
Kristof GILLIAERT
Eef VANKERCKHOVEN
Tania IVENS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biocartis NV
Original Assignee
Biocartis NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocartis NV filed Critical Biocartis NV
Publication of EP4701784A1 publication Critical patent/EP4701784A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP24725406.3A 2023-04-26 2024-04-26 Halbfestphase-extraktion von nukleinsäuren unter verwendung einer mischung aus quaternärem salz und kation unter alkalischen bedingungen Pending EP4701784A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL2034698A NL2034698B1 (en) 2023-04-26 2023-04-26 Semi-solid phase extraction of nucleic acids using a quaternary salt and cation mixture in alkaline conditions
PCT/EP2024/061528 WO2024223814A1 (en) 2023-04-26 2024-04-26 Semi-solid phase extraction of nucleic acids using a quaternary salt and cation mixture in alkaline conditions

Publications (1)

Publication Number Publication Date
EP4701784A1 true EP4701784A1 (de) 2026-03-04

Family

ID=86604237

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24725406.3A Pending EP4701784A1 (de) 2023-04-26 2024-04-26 Halbfestphase-extraktion von nukleinsäuren unter verwendung einer mischung aus quaternärem salz und kation unter alkalischen bedingungen

Country Status (3)

Country Link
EP (1) EP4701784A1 (de)
NL (1) NL2034698B1 (de)
WO (1) WO2024223814A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120172779A (zh) * 2025-02-26 2025-06-20 陕西中叶农业科技发展有限公司 一种缓释控释含多种营养元素的复肥及其制备方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US7001724B1 (en) * 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
WO2007002567A2 (en) * 2005-06-23 2007-01-04 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
JP5086250B2 (ja) 2005-06-23 2012-11-28 ビオカルティ ソシエテ アノニム 自動医療診断用のカートリッジ、システム及び方法
US8703476B2 (en) 2005-06-30 2014-04-22 Biocartis Sa Cartridge for automated medical diagnostics
KR20120018153A (ko) 2009-04-14 2012-02-29 비오까르띠 에스아 집속된 음향 에너지에 의한 샘플의 처리
US20120149088A1 (en) 2010-11-30 2012-06-14 Life Technologies Corporation Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples
EP3674406A1 (de) 2013-02-25 2020-07-01 Biocartis N.V. Isolierung von nukleinsäuren
CN107002077A (zh) 2014-11-07 2017-08-01 约翰霍普金斯大学 用于从血浆纯化核酸的无离液剂和无挥发物的方法
EP3061822A1 (de) * 2015-02-25 2016-08-31 QIAGEN GmbH Verfahren zum Konzentrieren, Isolieren und/oder Reinigen von Nukleinsäuren aus stark verdünnten wässrigen Proben
US20200063189A1 (en) 2017-02-27 2020-02-27 miDiagnostics NV System and method for purifying and amplifying nucleic acids
CA3149690A1 (en) 2019-08-08 2021-02-11 Biocartis Nv Novel nucleic acid purification chemistry
CN114457066B (zh) * 2021-12-10 2023-10-24 南京世和基因生物技术股份有限公司 一种dna提取方法、试剂盒以及构建的dna文库
CN115141832A (zh) 2022-06-22 2022-10-04 河南科技学院 tae-miR397在调控小麦抗病性中的应用
CN115141823A (zh) * 2022-07-20 2022-10-04 拜澳泰克(沈阳)生物医学集团有限公司 一种粪便微生物dna提取组合物、试剂盒及提取方法

Also Published As

Publication number Publication date
NL2034698B1 (en) 2024-11-04
WO2024223814A1 (en) 2024-10-31

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