EP4291647C0 - Subtilisinvarianten und ihre verwendung - Google Patents

Subtilisinvarianten und ihre verwendung

Info

Publication number
EP4291647C0
EP4291647C0 EP22704910.3A EP22704910A EP4291647C0 EP 4291647 C0 EP4291647 C0 EP 4291647C0 EP 22704910 A EP22704910 A EP 22704910A EP 4291647 C0 EP4291647 C0 EP 4291647C0
Authority
EP
European Patent Office
Prior art keywords
subtilisin variants
subtilisin
variants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP22704910.3A
Other languages
English (en)
French (fr)
Other versions
EP4291647A1 (de
EP4291647B1 (de
Inventor
Timo Nuijens
Jurgen Mateman
Visser Rowin De
Walter Cabri
Kerstin Wallraven
Elvira Wijker
Antonio Ricci
Ana Toplak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fresenius Kabi Ipsum SRL
Original Assignee
Fresenius Kabi Ipsum SRL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fresenius Kabi Ipsum SRL filed Critical Fresenius Kabi Ipsum SRL
Publication of EP4291647A1 publication Critical patent/EP4291647A1/de
Application granted granted Critical
Publication of EP4291647C0 publication Critical patent/EP4291647C0/de
Publication of EP4291647B1 publication Critical patent/EP4291647B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/10—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using coupling agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP22704910.3A 2021-02-12 2022-02-09 Subtilisinvarianten und ihre verwendung Active EP4291647B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP21156989 2021-02-12
EP21176039 2021-05-26
PCT/EP2022/053121 WO2022171667A1 (en) 2021-02-12 2022-02-09 Subtilisin variants and their use

Publications (3)

Publication Number Publication Date
EP4291647A1 EP4291647A1 (de) 2023-12-20
EP4291647C0 true EP4291647C0 (de) 2025-06-18
EP4291647B1 EP4291647B1 (de) 2025-06-18

Family

ID=81325357

Family Applications (1)

Application Number Title Priority Date Filing Date
EP22704910.3A Active EP4291647B1 (de) 2021-02-12 2022-02-09 Subtilisinvarianten und ihre verwendung

Country Status (4)

Country Link
EP (1) EP4291647B1 (de)
KR (1) KR20230144058A (de)
ES (1) ES3033476T3 (de)
WO (1) WO2022171667A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4525615A2 (de) 2022-05-14 2025-03-26 Novozymes A/S Zusammensetzungen und verfahren zur prävention, behandlung, unterdrückung und/oder beseitigung von phytopathogenen befällen und infektionen
CA3274855A1 (en) 2022-12-19 2024-06-27 Fresenius Kabi Ipsum S R L THERMOSABLE VARIANTS OF SUBTILISINE AND THEIR USE

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69131834T2 (de) 1990-08-09 2000-05-31 Genentech, Inc. Serinproteasevarianten mit peptidligase aktivität
CN1303437A (zh) * 1998-03-26 2001-07-11 宝洁公司 有氨基酸缺失和替代的丝氨酸蛋白酶变体
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US20100192985A1 (en) * 2008-11-11 2010-08-05 Wolfgang Aehle Compositions and methods comprising serine protease variants
KR101871728B1 (ko) 2014-07-08 2018-06-27 엘지전자 주식회사 3d 방송 서비스를 위한 방송 신호 송수신 방법 및 장치
CA2962903C (en) 2014-10-10 2023-01-10 Enzypep B.V. Peptide fragment condensation and cyclisation using a subtilisin variant with improved synthesis over hydrolysis ratio
EP3404036A1 (de) 2017-05-19 2018-11-21 Enzypep B.V. Subtilisinvarianten mit einer mutation in der s2- oder s2'-tasche
CN111757891B (zh) 2018-03-09 2024-09-27 费森尤斯卡比有限公司 索马鲁肽、利拉鲁肽和glp-1的化学-酶法合成
PL3704143T3 (pl) 2018-03-09 2024-04-15 Fresenius Kabi Ipsum S.R.L. Chemo-enzymatyczna synteza liraglutydu, semaglutydu i glp-1

Also Published As

Publication number Publication date
ES3033476T3 (en) 2025-08-04
WO2022171667A1 (en) 2022-08-18
EP4291647A1 (de) 2023-12-20
KR20230144058A (ko) 2023-10-13
EP4291647B1 (de) 2025-06-18
US20240043897A1 (en) 2024-02-08

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