EP4206193A1 - Nouveau dérivé biaryle utile en tant qu'inhibiteur de la diacylglycérol acyltransférase 2, et son utilisation - Google Patents

Nouveau dérivé biaryle utile en tant qu'inhibiteur de la diacylglycérol acyltransférase 2, et son utilisation Download PDF

Info

Publication number
EP4206193A1
EP4206193A1 EP21864708.9A EP21864708A EP4206193A1 EP 4206193 A1 EP4206193 A1 EP 4206193A1 EP 21864708 A EP21864708 A EP 21864708A EP 4206193 A1 EP4206193 A1 EP 4206193A1
Authority
EP
European Patent Office
Prior art keywords
phenyl
amino
pyrazin
ethoxyphenoxy
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21864708.9A
Other languages
German (de)
English (en)
Other versions
EP4206193A4 (fr
Inventor
Seung Hyun Yoon
Hyun Woo Joo
Bo Kyung Seo
Eun Jin Lee
Jin Young Jung
Su Young Yoon
Woo Young Cho
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LG Chem Ltd
Original Assignee
LG Chem Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LG Chem Ltd filed Critical LG Chem Ltd
Publication of EP4206193A1 publication Critical patent/EP4206193A1/fr
Publication of EP4206193A4 publication Critical patent/EP4206193A4/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
    • A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
    • A61K31/216—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acids having aromatic rings, e.g. benactizyne, clofibrate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/4965—Non-condensed pyrazines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/4965—Non-condensed pyrazines
    • A61K31/497—Non-condensed pyrazines containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/02—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings
    • C07D241/10—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D241/14—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D241/16—Halogen atoms; Nitro radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/02—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings
    • C07D241/10—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D241/14—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D241/20—Nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/10—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a carbon chain containing aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links

Definitions

  • the present invention relates to a biaryl derivative compound represented by Formula (1) showing inhibitory activity against diacylglycerol acyltransferase 2 (DGAT2), a pharmaceutical composition comprising the same as an active ingredient, and use thereof.
  • DGAT2 diacylglycerol acyltransferase 2
  • Neutral fats such as triglycerides (TG)
  • TG triglycerides
  • Diacylglycerol acyltransferase- which is a crucial enzyme in the biosynthesis of triglycerides-is found in various tissues of mammals, and is an enzyme that synthesizes TG by binding fatty acyl-CoA to the hydroxyl group of diacylglycerol in the final step of the glycerol phosphate pathway which is the main pathway for triglyceride synthesis.
  • two isoforms-DGAT1 and DGAT2- are known. Although their biochemical functions are similar, there is a difference in that DGAT1 is mainly expressed in the small intestine and adipose tissue, and DGAT2 is mainly expressed in the liver and adipose tissue.
  • DGAT1 belongs to the ACAT family
  • DGAT2 belongs to the MGAT family. As such, it is expected that their role in TG biosynthesis is also different.
  • DGAT2 primarily contributes to the biosynthesis of TG in vivo. Unlike DGAT2 knockout mice-which hardly synthesize TG and die shortly after birth due to an abnormal skin layer, DGAT1 knockout mice showed a slight decrease in TG levels and no problems with the survival of the mice ( Stone SJ et al., 2000. Nat. Genet. 25: 87-90 ).
  • DGAT2 hepatic VLDL TAG secretion and reduced circulating cholesterol levels.
  • APOB plasma apolipoprotein B
  • An object of the present invention is to provide a novel biaryl derivative compound represented by Formula (1) showing inhibitory activity against diacylglycerol acyltransferase 2 (DGAT2).
  • Another object of the present invention is to provide a method of preparing the biaryl derivative compound.
  • Still another object of the present invention is to provide a pharmaceutical composition for the treatment of metabolic diseases associated with DGAT2 comprising the biaryl derivative compound as an active ingredient, and a method for preparing thereof.
  • Still another object of the present invention is to provide a method for treating metabolic diseases associated with DGAT2 in a subject in which efficacy in animal models of diseases is improved as well as efficacy and convenience in taking in the subject are improved by using the biaryl derivative compound as an active ingredient having improve physical and chemical properties compared to conventional compounds.
  • the present invention provides a compound of the following Formula (1), or a pharmaceutically acceptable salt or isomer: wherein
  • a pharmaceutically acceptable salt may include an acid-addition salt which is formed from an inorganic acid such as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrobromic acid and hydroiodic acid; an organic acid such as tartaric acid, formic acid, citric acid, acetic acid, trichloroacetic acid, trifluoroacetic acid, gluconic acid, benzoic acid, lactic acid, fumaric acid, maleic acid and salicylic acid; or sulfonic acid such as methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid and p-toluenesulfonic acid, which form non-toxic acid-addition salt including pharmaceutically acceptable anion.
  • an inorganic acid such as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrobromic acid and hydroiodic acid
  • an organic acid such as tartaric acid, formic acid, citric
  • a pharmaceutically acceptable carboxylic acid salt includes the salt with alkali metal or alkali earth metal such as lithium, sodium, potassium, calcium and magnesium; salts with amino acid such as lysine, arginine and guanidine; an organic salt such as dicyclohexylamine, N-methyl-D-glucamine, tris(hydroxymethyl)methylamine, diethanolamine, choline and triethylamine.
  • alkali metal or alkali earth metal such as lithium, sodium, potassium, calcium and magnesium
  • salts with amino acid such as lysine, arginine and guanidine
  • an organic salt such as dicyclohexylamine, N-methyl-D-glucamine, tris(hydroxymethyl)methylamine, diethanolamine, choline and triethylamine.
  • the compound of Formula (1) according to the present invention may be converted into their salts by conventional methods.
  • the compound of Formula (1) according to the present invention can have an asymmetric carbon center and asymmetric axis or plane, they can exist as E- or Z-isomer, R- or S-isomer, racemic mixtures or diastereoisomer mixtures and each diastereoisomer, all of which are within the scope of the present invention.
  • the term "the compound of Formula (1)” is used to mean all the compounds of Formula (1), including the pharmaceutically acceptable salts and isomers thereof.
  • halogen or halo means fluoride (F), chlorine (Cl), bromine (Br) or iodine (I).
  • alkyl or "alkylene” means straight or branched hydrocarbons, may include a single bond, a double bond or a triple bond, and is preferably C 1 -C 10 alkyl or C 1 -C 10 alkylene, or C 1 -C 7 alkyl or C 1 -C 7 alkylene.
  • alkyl include, but are not limited to, methyl, ethyl, n -propyl, i -propyl, n -butyl, i -butyl, tert -butyl, acetylene, vinyl, trifluoromethyl and the like.
  • alkenyl or "alkenylene” means straight or branched hydrocarbons having at least one carbon-carbon double bond, and is preferably C 2 -C 10 alkenyl or C 2 -C 10 alkenylene, or C 2 -C 7 alkenyl or C 2 -C 7 alkenylene.
  • alkenyl include, but are not limited to, vinyl, allyl, butenyl, isopropenyl, isobutenyl and the like.
  • cycloalkyl means partially or fully saturated single or fused ring hydrocarbons, and is preferably C 3 -C 10 -cycloalkyl.
  • examples of cycloalkyl include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and the like.
  • alkoxy means alkyloxy having 1 to 10 carbon atoms.
  • aryl or "arylene” means aromatic hydrocarbons, preferably C 5 -C 12 aryl or C 5 -C 12 arylene, more preferably C 6 -C 10 aryl or C 6 -C 10 arylene, and includes, but is not limited to, phenyl, naphthyl and the like.
  • heteroaryl or “heteroarylene” means 3- to 12-membered, more preferably 5- to 12-membered aromatic hydrocarbons which form a single or fused ring-which may be fused with benzo or C 3 -C 8 cycloalkyl-including one or more heteroatoms selected from N, O and S as a ring member.
  • heteroaryl examples include, but are not limited to, pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, oxadiazolyl, isoxadiazolyl, tetrazolyl, triazolyl, indolyl, indazolyl, isoxazolyl, oxazolyl, thiazolyl, isothiazolyl, furanyl, benzofuranyl, imidazolyl, thiophenyl, benzthiazole, benzimidazole, quinolinyl, indolinyl, 1,2,3,4-tetrahydroisoquinolyl, 3,4-dihydroisoquinolinyl, thiazolopyridyl, 2,3-dihydrobenzofuran, 2,3-dihydrothiophene, 2,3-dihydroindole, benzo[1,3]dioxin, chroman, thiochroman
  • heterocycloalkyl or “heterocycloalkylene” means partially or fully saturated hydrocarbons which form a single or fused ring including one or more heteroatoms selected from N, O and S, and is preferably 3- to 12-membered heterocycloalkyl or heterocycloalkylene, or 5- to 12-membered heterocycloalkyl or heterocycloalkylene.
  • heterocycloalkyl or heterocycloalkylene include, but are not limited to, pyrrolidinyl, piperidinyl, morpholinyl, imidazolinyl, piperazinyl, tetrahydrofuran, tetrahydrothiofuran and the like.
  • Representative compounds of Formula (1) according to the present invention include, but are not limited to, the following compounds:
  • the present invention also provides a method for preparing the compound of Formula (1).
  • the method for preparing the compound of Formula (1) is explained based on exemplary reactions in order to illustrate the present invention.
  • a person skilled in the art could prepare the compound of Formula (1) by various methods based on the structure of Formula (1), and such methods should be interpreted as being within the scope of the present invention. That is, the compound of Formula (1) may be prepared by the methods described herein or by combining various methods disclosed in the prior art, which should be interpreted as being within the scope of the present invention. Accordingly, a method for preparing the compound of Formula (1) is not limited to the following methods.
  • the compound of formula (1) of the present invention may be prepared by directly introducing a substituted amine group into compound (2), or introducing a protected amine into compound (2), removing the protecting group to obtain compound (3) and carrying out an amidation reaction on compound (3), according to the method of Reaction Scheme 1 below.
  • Compound (2) may be prepared by using 2-ethoxyphenol as a starting material according to the method of Reaction Scheme 2 below.
  • compound (3) may be prepared according to the method of Reaction Scheme 3 below.
  • amide derivatives may be obtained by the treatment with thionyl chloride or oxalyl chloride from an appropriate acid followed by the treatment with ammonia water.
  • methyl 4-(3-amino-3-oxopropyl)benzoate may be prepared according to the method of Reaction Scheme 4 below.
  • amine derivatives may be obtained by introducing an amino group into a compound obtained through a cross-coupling reaction between a dioxaborolane core intermediate and various kinds of chloro aryl compounds to synthesize amino aryl intermediates.
  • ethyl 2-(4-(2-aminopyrimidin-4-yl)phenyl)acetate may be prepared according to the method of Reaction Scheme 5 below.
  • a compound not specifically described in the preparation method of the present specification is a known compound or a compound that can be easily synthesized from a known compound by a known synthesis method or a similar method.
  • the compound of Formula (1) obtained by the above methods can be separated or purified from the reaction products by conventional methods such as recrystallization, ionospheresis, silica gel column chromatography or ion-exchange chromatography.
  • the compounds according to the present invention can be prepared by a variety of methods, which should be interpreted as being within the scope of the present invention in respect to the preparation of the compound of Formula (1).
  • the compound of Formula (1) according to the present invention exhibits inhibitory activity against diacylglycerol acyltransferase 2 (DGAT2). Accordingly, the present invention provides a pharmaceutical composition for the treatment of diseases associated with DGAT2 comprising the compound of Formula (1), or a pharmaceutically acceptable salt or isomer thereof, together with a pharmaceutically acceptable carrier.
  • DGAT2 diacylglycerol acyltransferase 2
  • the present invention provides a pharmaceutical composition for the treatment of diseases associated with DGAT2 comprising the compound of Formula (1), or a pharmaceutically acceptable salt or isomer thereof, together with a pharmaceutically acceptable carrier.
  • Various kinds of prodrugs, which are converted into the compound of Formula (1) in vivo are also within the scope of the present invention.
  • Exemplary diseases associated with DGAT2 which can be treated by the pharmaceutical composition according to the present invention include, but are not limited to, that selected from the group consisting of fatty liver, nonalcoholic steatohepatitis (NASH), nonalcoholic fatty liver disease (NAFLD), diabetes, obesity, hyperlipidemia, atherosclerosis and hypercholesterolemia.
  • NASH nonalcoholic steatohepatitis
  • NAFLD nonalcoholic fatty liver disease
  • a "pharmaceutical composition” may include other components such as carriers, diluents, excipients, etc., in addition to the active ingredient of the present invention. Accordingly, the pharmaceutical composition may include pharmaceutically acceptable carriers, diluents, excipients or combinations thereof, if necessary.
  • the pharmaceutical composition facilitates the administration of compounds into the body. Various methods for administering the compounds include, but are not limited to, oral, injection, aerosol, parenteral and local administration.
  • a “carrier” means a compound that facilitates the addition of compounds into the cell or tissue.
  • DMSO dimethylsulfoxide
  • DMSO dimethylsulfoxide
  • a "diluent” means a compound that not only stabilizes a biologically active form but is diluted in solvent dissolving the compounds.
  • a dissolved salt in buffer is used as a diluent in this field.
  • a conventionally used buffer is a phosphate buffer saline mimicking salt form in body fluid. Since a buffer solution can control the pH of the solution at low concentration, a buffer diluent hardly modifies the biological activity of compounds.
  • pharmaceutically acceptable means such property that does not impair the biological activity and physical property of compounds.
  • the compounds according to the present invention can be formulated as various pharmaceutically administered dosage forms.
  • an active component-specifically, the compound of Formula (1) or a pharmaceutically acceptable salt or isomer thereof- is mixed with selected pharmaceutically acceptable carriers considering the dosage form to be prepared.
  • the pharmaceutical composition of the present invention can be formulated as injections, oral preparations and the like, as needed.
  • the compound of the present invention can be formulated by conventional methods using known pharmaceutical carriers and excipients, and inserted into a unit or multi-unit containers.
  • the formulations may be solution, suspension or emulsion in oil or aqueous solvent and include conventional dispersing agents, suspending agents or stabilizing agents.
  • the compound may be, for example, dry powder form which is dissolved in sterilized pyrogen-free water before use.
  • the compound of the present invention can be formulated into suppositories by using a conventional suppository base such as cocoa butter or other glycerides.
  • Solid forms for oral administration include capsules, tablets, pills, powders and granules. Capsules and tablets are preferred. Tablets and pills are preferably enteric-coated.
  • Solid forms are manufactured by mixing the compounds of the present invention with at least one carrier selected from inert diluents such as sucrose, lactose or starch, lubricants such as magnesium stearate, disintegrating agents, binders and the like.
  • carrier selected from inert diluents such as sucrose, lactose or starch, lubricants such as magnesium stearate, disintegrating agents, binders and the like.
  • the compound or a pharmaceutical composition comprising the same according to the present invention can be administered in combination with other drugs-for example, other metabolic disorder therapeutic agents-as required.
  • the dose of the compound of Formula (1) according to the present invention is determined by a physician's prescription considering the patient's body weight, age and disease condition.
  • a typical dose for adults is in the range of about 0.3 to 500 mg per day according to the frequency and intensity of administration.
  • a typical daily dose of intramuscular or intravenous administration for adults is in the range of about 1 to 300 mg per day which can be administered in divided unit dosages. Some patients need a higher daily dose.
  • treatment is used to mean deterring, delaying or ameliorating the progress of diseases in a subject exhibiting symptoms of diseases.
  • the novel biaryl derivative compound of Formula (1) according to the present invention exhibits excellent inhibitory activity against diacylglycerol acyltransferase 2 (DGAT2), and thus can be usefully used in the prevention, alleviation or treatment of metabolic disorders associated with DGAT2.
  • DGAT2 diacylglycerol acyltransferase 2
  • the novel biaryl derivative compound of Formula (1) according to the present invention exhibits increased lipophilicity and liver selectivity, thereby improving efficacy through increased exposure to the liver, as well as expecting the advantages of convenience in taking because the half-life is relatively long in disease animal models and clinical practice.
  • M refers to molar concentration
  • N refers to normal concentration
  • Step 3 Synthesis of methyl 2-(4-carbamoylphenoxy)-2-methylpropanoate
  • Step 1 Synthesis of tert -butyl ( E )-3-(4-(2-methoxy-2-oxoethyl)phenyl)acrylate
  • Methyl 2-(4-bromophenyl)acetate (16.4 g, 71.56 mmol), tert -butyl acrylate (18.0 g, 143.0 mmol) and triethylamine (50 mL, 0.35 mol) were dissolved in 200 ml of dimethylformamide. After removing dissolved oxygen through nitrogen bubbling, bis(triphenylphosphine)palladium dichloride (2.5 g, 3.58 mmol) was added dropwise and stirred at 75°C for 12 hours. The organic solvent was removed under reduced pressure, diluted with ethyl acetate, washed with brine, dried over magnesium sulfate, and then the organic solvent was removed under reduced pressure.
  • Step 2 Synthesis of tert -butyl 3-(4-(2-methoxy-2-oxoethyl)phenyl)propanoate
  • Step 4 Synthesis of methyl 2-(4-(3-amino-3-oxopropyl)phenyl)acetate
  • Step 1 Synthesis of tert -butyl ( E )-3-(4-(1-methoxy-2-methyl-1-oxopropan-2-yl)phenyl)acrylate
  • Step 2 Synthesis of methyl 2-(4-(3-( tert -butoxy)-3-oxopropyl)phenyl)-2-methylpropanoate
  • Step 3 Synthesis of 3-(4-(1-methoxy-2-methyl-1-oxopropan-2-yl)phenyl)propanoic acid
  • Step 4 Synthesis of methyl 2-(4-(3-amino-3-oxopropyl)phenyl)-2-methylpropanoate
  • Step 1 Synthesis of tert -butyl 2-(4-iodophenyl)acetate
  • Step 2 Synthesis of ethyl 2-(4-(2-( tert -butoxy)-2-oxoethyl)phenyl)-2,2-difluoroacetate
  • Step 3 Synthesis of 2-(4-(2-ethoxy-1,1-difluoro-2-oxoethyl)phenyl)acetic acid
  • Step 1 Synthesis of 2-(4-(3- tert -butoxy-2,2-dimethyl-3-oxopropyl)phenyl)acetic acid
  • reaction solution was stirred at - 78°C for 1 hour and slowly added dropwise to 2-(4-(bromomethyl)phenyl)acetic acid (7.5 g, 32.7 mmol) dissolved in anhydrous tetrahydrofuran (163 ml).
  • the reaction solution was heated to room temperature and stirred for 20 minutes.
  • the reaction was terminated by adding 1 N aqueous hydrochloric acid (100 ml) to the reaction solution, followed by extraction with diethyl ether.
  • Step 2 Synthesis of tert -butyl 3-(4-(2-amino-2-oxoethyl)phenyl)-2,2-dimethylpropanoate
  • Step 1 Synthesis of ethyl ( R )-1-(2-chloropyrimidin-4-yl)piperidine-3-carboxylate
  • 2,4-Dichloropyrimidine (0.5 g, 3.36 mmol) was dissolved in ethanol (6.71 ml), and ethyl ( R )-piperidine-3-carboxylate (0.621 ml, 4.03 mmol) and TEA (0.187 ml, 1.343 mmol) were added thereto.
  • the reaction mixture was stirred at 85°C for 3 hours. After removing the solvent under reduced pressure, the resultant product was dissolved in ethyl acetate and washed with water.
  • the purification was carried out by silica gel column to obtain the desired product (yield: 86%).
  • Step 2 Synthesis of ethyl ( R )-1-(2-((tert-butoxycarbonyl)amino)pyrimidin-4-yl)piperidine-3-carboxylate
  • Step 3 Synthesis of ethyl ( R )-1-(2-aminopyrimidin-4-yl)piperidine-3-carboxylate
  • Step 1 Synthesis of tert -butyl 3-(3-bromophenyl)-2,2-dimethylpropanoate
  • Step 2 Synthesis of tert -butyl 2,2-dimethyl-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl-propanoate
  • Step 3 Synthesis of tert -butyl 3-(3-(6-aminopyridin-2-yl)-phenyl)-2,2-dimethylpropanoate
  • Step 1 Synthesis of tert -butyl 2-(4-(3-methoxy-3-oxopropyl)phenoxy)-2-methylpropanoate
  • Step 2 Synthesis of 3-(4-((1-( tert -butoxy)-2-methyl-1-oxopropan-2-yl)oxy)phenyl)propanoic acid
  • Step 3 Synthesis of tert -butyl 2-(4-(3-amino-3-oxopropyl)phenoxy)-2-methylpropanoate
  • Step 1 Synthesis of tert -butyl 3-(4-(1-methoxy-2-methyl-1-oxopropan-2-yl)phenyl)-2,2-dimethylpropanoate
  • Step 2 Synthesis of tert -butyl 3-(4-(1-amino-2-methyl-1-oxopropan-2-yl)phenyl)-2,2-dimethylpropanoate
  • Step 1 Synthesis of methyl (1 r ,4 r )-4-hydroxycyclohexane-1-carboxylate
  • Step 2 Synthesis of methyl (1 r ,4 r )-4-((2-chloropyrimidin-4- yl)oxy)cyclohexane-1-carboxylate
  • Step 3 Synthesis of methyl (1 r ,4 r )-4-((2-aminopyrimidin-4- yl)oxy)cyclohexane-1-carboxylate
  • Step 1 Synthesis of methyl 2-(4-(3-((6-(5-(2-ethoxyphenoxy)pyridin-3-yl)amino)-3-oxopropyl)phenyl)acetate
  • Step 2 Synthesis of 2-(4-(3-((6-(5-(2-ethoxyphenoxy)pyridin-3-yl)pyrazin-2-yl)amino)-3-oxopropyl)phenyl)acetic acid
  • Step 2 Synthesis of ethyl 2-(4-(2-((6-(5-(2-ethoxyphenoxy)pyridin-3-yl)pyrazin-2-yl)amino)-2-oxoethyl)phenyl)-2,2-difluoroacetate
  • Step 1 Synthesis of methyl 2-(4-(3-((6-(3-(2-ethoxyphenoxy)phenyl)pyrazin-2-yl)amino)-3-oxopropyl)phenyl)acetate
  • Step 2 Synthesis of 2-(4-(3-((6-(3-(2-ethoxyphenoxy)phenyl)pyrazin-2-yl)amino)-3-oxopropyl)phenyl)acetic acid
  • Step 2 Synthesis of 2-(4-(2-((6-(3-(2-ethoxyphenoxy)phenyl)pyrazin-2-yl)amino)-2-oxoethyl)phenyl-2,2-difluoroacetic acid
  • Example 17 Synthesis of ( E )-2-(4-(3-((6-(3-(2-ethoxyphenoxy)phenyl)pyrazin-2-yl)amino)-3-oxopro-1-phen-1-yl)phenyl)-2-methylpropanoic acid
  • the inhibitory effect against the DGAT2 enzyme activity was investigated by performing the following experiment on the compounds of Formula (1) according to the present invention.
  • pBacPAK9-DGAT2 which is DGAT2 expression vector
  • human DGAT2 gene amplified by polymerase chain reaction (PCR) was cloned into the EcoR1 and Xho1 sites of the pBacPAK9 (clonctech) vector.
  • the nucleotide sequence of the primers used in PCR was the forward primer 5' CTATAAATACGGATCCCGGGAATTCATGGACTACAAGGACGACGATGACAAG CTTAAGACCCTCATAGCCGCC and the reverse primer 5' TAAGCGGCCGCCCTGCAGGCCTCGAGTCAGTTCACCTCCAGGAC.
  • the composition of the reaction solution was to contain 50 ng of cDNA clone (OriGene), 200 ⁇ M of dATP, dCTP, dTTP, dGTP, 200 nM of each primer, 1 unit of Tag DNA Polymerase (Toyobo), 1x PCR buffer, and the final volume was adjusted to 20 ⁇ l.
  • the reaction conditions were denatured at 95°C for 5 minutes, followed by 30 times of 94°C for 20 seconds, 60°C for 20 seconds, and 72°C for 90 seconds, followed by further reaction at 72°C for 7 minutes.
  • Recombinant human DGAT2 protein was expressed in Sf-21 cells, which are insect cells, by using the BacPack baculovirus expression system (Clontech).
  • the brief manufacturing process is as follows. First, the pBacPAK9-DGAT2 expression vector was transfected with BacPAK6 virus DNA (Bsu36I digest) into sf21 cells using Bacfectin to prepare a recombinant DGAT2 expressing baculovirus. The thus prepared baculovirus was infected with Sf-21 cells at 10 MOI (multiplicity of infection), and after 72 hours, infected insect cells were collected and membrane proteins were isolated.
  • the cell pellet was dissolved in a sucrose solution containing 250 mM sucrose, 10 mM Tris (pH 7.4), and 1 mM ethylenediaminetetraacetic acid (EDTA), and then homogenized by using a dounce homogenizer, and the supernatant was taken by centrifuging at 600 ⁇ g for 15 minutes, and centrifuged at 100,000 ⁇ g for 1 hour to discard the supernatant, and the remaining pellet was resuspended in 20 mM HEPES buffer (pH 7.4).
  • the prepared DGAT2 overexpressing membrane protein was dispensed in 100 ⁇ l and stored at -80°C until use. Protein concentration was quantified by using the BCA Protein Assay Kit (Thermo Scientific).
  • DGAT2 inhibition compounds serially diluted 5 times from 3 nM to 10 ⁇ M (final concentration, 1% DMSO) were mixed in a buffer solution containing 2 ⁇ g DGAT2-membrane protein and 20 mM HEPES, 20 mM MgCl 2 , 1 mg/mL BSA, 50 ⁇ M 1,2 sn- oleoyl glycerol (Sigma), put in a 96-well flash plate (FlashPlate) and reacted at 37°C for 20 minutes, and then 1 ⁇ M [14C] ole oil CoA (PerkinElmer, NEC651050UC) was added to be a final volume of 100 ⁇ L and further reacted at 37°C for 15 minutes.
  • SPA Stintilation Proximity Assay
  • IC 50 value which is the concentration of the compound that inhibits TG production by 50%, was determined by treating the response value according to the compound concentration with a nonlinear regression curve using PRISM (Graphpad Inc.).
  • Example IC 50 ( ⁇ M) Example IC 50 ( ⁇ M)
  • Example IC 50 ( ⁇ M) 1 0.035 12 0.17 23 0.061 2 0.027 13 0.33 24 7.4 3 3.4 14 3.1 25 0.45 4 0.29 15 0.013 26 0.021 5 0.011 16 0.021 27 0.0074 6 0.12 17 0.24 28 0.21 7 0.036 18 0.022 29 0.0067 8 1.8 19 0.43 30 0.25 9 0.16 20 3.9

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Epidemiology (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Urology & Nephrology (AREA)
  • Child & Adolescent Psychology (AREA)
  • Endocrinology (AREA)
  • Vascular Medicine (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Plural Heterocyclic Compounds (AREA)
EP21864708.9A 2020-09-04 2021-09-03 Nouveau dérivé biaryle utile en tant qu'inhibiteur de la diacylglycérol acyltransférase 2, et son utilisation Pending EP4206193A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20200112843 2020-09-04
PCT/KR2021/011906 WO2022050749A1 (fr) 2020-09-04 2021-09-03 Nouveau dérivé biaryle utile en tant qu'inhibiteur de la diacylglycérol acyltransférase 2, et son utilisation

Publications (2)

Publication Number Publication Date
EP4206193A1 true EP4206193A1 (fr) 2023-07-05
EP4206193A4 EP4206193A4 (fr) 2024-03-13

Family

ID=80491243

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21864708.9A Pending EP4206193A4 (fr) 2020-09-04 2021-09-03 Nouveau dérivé biaryle utile en tant qu'inhibiteur de la diacylglycérol acyltransférase 2, et son utilisation

Country Status (11)

Country Link
US (1) US20230322706A1 (fr)
EP (1) EP4206193A4 (fr)
JP (1) JP2023540337A (fr)
KR (1) KR102650118B1 (fr)
CN (1) CN116056706A (fr)
AU (1) AU2021337476A1 (fr)
BR (1) BR112023003744A2 (fr)
CA (1) CA3189681A1 (fr)
MX (1) MX2023002429A (fr)
TW (1) TWI817191B (fr)
WO (1) WO2022050749A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2022007810A (es) * 2019-12-23 2022-07-19 Lg Chemical Ltd Derivado de amida novedoso util como inhibidor de diacilglicerol aciltransferasa 2, y uso del mismo.

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2196465A1 (fr) * 2008-12-15 2010-06-16 Almirall, S.A. Dérivés de (3-oxo)pyridazin-4-ylurée comme inhibiteurs de PDE4
WO2011031628A1 (fr) * 2009-09-14 2011-03-17 Schering Corporation Inhibiteurs de diacylglycérol acyltransférase
KR101464429B1 (ko) * 2012-03-16 2014-11-27 한국생명공학연구원 신규한 디아실글리세롤 아실트랜스퍼레이즈 2 억제 물질 및 이의 이용
CA2869587A1 (fr) * 2012-04-06 2013-10-10 Pfizer Inc. Inhibiteurs de diacylglycerol acyltransferase 2
GEP20186864B (en) * 2014-03-17 2018-06-25 Pfizer Diacylglycerol acyltransferase 2 inhibitors for use in the treatment of metabolic and related disorders
AR109179A1 (es) * 2016-08-19 2018-11-07 Pfizer Inhibidores de diacilglicerol aciltransferasa 2

Also Published As

Publication number Publication date
CA3189681A1 (fr) 2022-03-10
KR102650118B1 (ko) 2024-03-21
TWI817191B (zh) 2023-10-01
US20230322706A1 (en) 2023-10-12
WO2022050749A1 (fr) 2022-03-10
MX2023002429A (es) 2023-03-22
BR112023003744A2 (pt) 2023-03-28
CN116056706A (zh) 2023-05-02
KR20220031521A (ko) 2022-03-11
JP2023540337A (ja) 2023-09-22
AU2021337476A1 (en) 2023-05-18
TW202225153A (zh) 2022-07-01
EP4206193A4 (fr) 2024-03-13

Similar Documents

Publication Publication Date Title
AU2016328150B2 (en) Prodrugs of gamma-hydroxybutyric acid, compositions and uses thereof
JP6449482B2 (ja) 縮合環誘導体、その製造方法、中間体、薬学的組成物及び応用
US10456405B2 (en) Nitric oxide-releasing prodrug molecule of substituted quinazolines
KR20230104589A (ko) 화합물, 알데히드 디히드로게나아제 2 활성화제, 의약 조성물 및 치료 및/또는 예방약
EP3719010B1 (fr) Composé aromatique, composition pharmaceutique et utilisation associées
KR102650118B1 (ko) 디아실글리세롤 아실트랜스퍼라제 2 억제제로서 유용한 신규 바이아릴 유도체 및 이의 용도
RU2808433C1 (ru) Новое биарильное производное, применяемое в качестве ингибитора диацилглицерол-ацилтрансферазы 2, и его применение
JP2018522068A (ja) Ｉｄｈ２突然変異を標的とする抗腫瘍化合物及びその使用方法
JP7438602B2 (ja) ジアシルグリセロールアシルトランスフェラーゼ２抑制剤として有用な新規アミノアリール誘導体及びその使用
EP4074701B1 (fr) Nouveau dérivé d'amide utile en tant qu'inhibiteur de la diacylglycérol acyltransférase 2, et son utilisation
RU2799819C1 (ru) Новое аминоарильное производное, пригодное в качестве ингибитора диацилглицеролацилтрансферазы 2, и его применение
RU2810064C1 (ru) Новое амидное производное, используемое в качестве ингибитора диацилглицерол o-ацилтрансферазы 2, и его применение
WO2023204292A1 (fr) Composé, activateur d'aldéhyde déshydrogénase 2, composition pharmaceutique et traitement et/ou médicament préventif
CN113924088A (zh) 同位素稳定的特窗酰亚胺化合物

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20230329

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20240208

RIC1 Information provided on ipc code assigned before grant

Ipc: C07D 241/16 20060101ALI20240202BHEP

Ipc: A61P 9/10 20060101ALI20240202BHEP

Ipc: A61P 3/06 20060101ALI20240202BHEP

Ipc: A61P 3/04 20060101ALI20240202BHEP

Ipc: A61P 3/10 20060101ALI20240202BHEP

Ipc: A61P 1/16 20060101ALI20240202BHEP

Ipc: A61K 31/216 20060101ALI20240202BHEP

Ipc: A61K 31/497 20060101ALI20240202BHEP

Ipc: A61K 31/4965 20060101ALI20240202BHEP

Ipc: C07D 401/04 20060101AFI20240202BHEP