EP4048802A4 - Transgenes schwein, verfahren zu dessen herstellung und verwendung und verfahren zur herstellung von mäusen des menschlichen immunsystems - Google Patents

Transgenes schwein, verfahren zu dessen herstellung und verwendung und verfahren zur herstellung von mäusen des menschlichen immunsystems Download PDF

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Publication number
EP4048802A4
EP4048802A4 EP20880160.5A EP20880160A EP4048802A4 EP 4048802 A4 EP4048802 A4 EP 4048802A4 EP 20880160 A EP20880160 A EP 20880160A EP 4048802 A4 EP4048802 A4 EP 4048802A4
Authority
EP
European Patent Office
Prior art keywords
methods
obtaining
immune system
human immune
mice
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20880160.5A
Other languages
English (en)
French (fr)
Other versions
EP4048802A1 (de
Inventor
Megan Sykes
Robert J. Hawley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Columbia University in the City of New York
Original Assignee
Columbia University in the City of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Columbia University in the City of New York filed Critical Columbia University in the City of New York
Publication of EP4048802A1 publication Critical patent/EP4048802A1/de
Publication of EP4048802A4 publication Critical patent/EP4048802A4/de
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/26—Lymph; Lymph nodes; Thymus; Spleen; Splenocytes; Thymocytes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/12—Animals modified by administration of exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • A01K2267/0387—Animal model for diseases of the immune system

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Materials For Medical Uses (AREA)
EP20880160.5A 2019-10-22 2020-10-22 Transgenes schwein, verfahren zu dessen herstellung und verwendung und verfahren zur herstellung von mäusen des menschlichen immunsystems Pending EP4048802A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962924228P 2019-10-22 2019-10-22
US201962925859P 2019-10-25 2019-10-25
PCT/US2020/056771 WO2021081156A1 (en) 2019-10-22 2020-10-22 Transgenic swine, methods of making and uses thereof, and methods of making human immune system mice

Publications (2)

Publication Number Publication Date
EP4048802A1 EP4048802A1 (de) 2022-08-31
EP4048802A4 true EP4048802A4 (de) 2023-11-22

Family

ID=75620265

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20880160.5A Pending EP4048802A4 (de) 2019-10-22 2020-10-22 Transgenes schwein, verfahren zu dessen herstellung und verwendung und verfahren zur herstellung von mäusen des menschlichen immunsystems

Country Status (9)

Country Link
US (1) US20220279767A1 (de)
EP (1) EP4048802A4 (de)
JP (2) JP7685249B2 (de)
KR (1) KR20220084296A (de)
CN (1) CN114585744A (de)
AU (1) AU2020370254B2 (de)
CA (1) CA3155234A1 (de)
IL (1) IL292406A (de)
WO (1) WO2021081156A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10883084B2 (en) 2018-10-05 2021-01-05 Xenotherapeutics, Inc. Personalized cells, tissues, and organs for transplantation from a humanized, bespoke, designated-pathogen free, (non-human) donor and methods and products relating to same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6639122B1 (en) * 2000-09-19 2003-10-28 Animal Technology Institute Taiwan Transgenic swine having HLA-D gene, swine cells thereof and xenografts therefrom
WO2012092578A1 (en) * 2010-12-31 2012-07-05 The Trustees Of Columbia University In The City Of New York Generation of autologous t-cells in mice
EP3058819A1 (de) * 2013-10-18 2016-08-24 Taiho Pharmaceutical Co., Ltd. Hla-klasse i exprimierendes nichtmenschliches tier
WO2016210280A1 (en) * 2015-06-26 2016-12-29 Indiana University Research & Technology Corporation Transgenic pigs with genetic modifications of sla

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2227584A1 (en) * 1995-08-04 1997-02-20 The General Hospital Corporation Transgenic swine and swine cells having human hla genes
WO2012145384A1 (en) * 2011-04-20 2012-10-26 University Of Washington Through Its Center For Commercialization Beta-2 microglobulin-deficient cells
DK3590332T3 (da) * 2011-10-28 2022-05-23 Regeneron Pharma Genmodificerede mus, der eksprimerer kimære major histokompatibilitetskompleks (MHC) II-molekyler
AU2015360502A1 (en) * 2014-12-10 2017-06-29 Regents Of The University Of Minnesota Genetically modified cells, tissues, and organs for treating disease
MX394999B (es) * 2017-09-29 2025-03-24 Regeneron Pharma Animales no humanos que comprenden un locus ttr humanizado y metodos de uso
KR20210089659A (ko) * 2018-10-05 2021-07-16 제노세라퓨틱스, 인코포레이티드 이종이식 생성물 및 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6639122B1 (en) * 2000-09-19 2003-10-28 Animal Technology Institute Taiwan Transgenic swine having HLA-D gene, swine cells thereof and xenografts therefrom
WO2012092578A1 (en) * 2010-12-31 2012-07-05 The Trustees Of Columbia University In The City Of New York Generation of autologous t-cells in mice
EP3058819A1 (de) * 2013-10-18 2016-08-24 Taiho Pharmaceutical Co., Ltd. Hla-klasse i exprimierendes nichtmenschliches tier
WO2016210280A1 (en) * 2015-06-26 2016-12-29 Indiana University Research & Technology Corporation Transgenic pigs with genetic modifications of sla

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KHOSRAVI MAHARLOOEI M ET AL: "Generation of human/pig hybrid thymus to achieve immune tolerance to pig antigens with optimal immune function", AMERICAN JOURNAL OF TRANSPLANTATION, US, vol. 19, no. Supplement 3, April 2019 (2019-04-01), pages 1052, XP002806800, ISSN: 1600-6143 *
KUDVA YOGISH C ET AL: "HLA-DQ8 transgenic and NOD mice recognize different epitopes within the cytoplasmic region of the tyrosine phosphatase-like molecule, IA-2", vol. 62, no. 10, October 2001 (2001-10-01), US, pages 1099 - 1105, XP093090823, ISSN: 0198-8859, Retrieved from the Internet <URL:https://www.sciencedirect.com/science/article/pii/S0198885901003081/pdfft?md5=c0d3c1fc53fea456df4cbc1d463f5238&pid=1-s2.0-S0198885901003081-main.pdf> DOI: 10.1016/S0198-8859(01)00308-1 *
LE M. T. ET AL: "Comprehensive and high-resolution typing of swine leukocyte antigen DQA from genomic DNA and determination of 25 new SLA class II haplotypes : Analysis of SLA-DQA polymorphisms using genomic DNA-based high-resolution typing", TISSUE ANTIGENS., vol. 80, no. 6, 9 November 2012 (2012-11-09), DK, pages 528 - 535, XP093090828, ISSN: 0001-2815, DOI: 10.1111/tan.12017 *
See also references of WO2021081156A1 *

Also Published As

Publication number Publication date
CN114585744A (zh) 2022-06-03
IL292406A (en) 2022-06-01
JP2022553328A (ja) 2022-12-22
CA3155234A1 (en) 2021-04-29
AU2020370254A1 (en) 2022-04-14
US20220279767A1 (en) 2022-09-08
WO2021081156A1 (en) 2021-04-29
EP4048802A1 (de) 2022-08-31
JP7685249B2 (ja) 2025-05-29
KR20220084296A (ko) 2022-06-21
JP2025109839A (ja) 2025-07-25
AU2020370254B2 (en) 2026-04-23

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