Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
  • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
  • A61K8/9783—Angiosperms [Magnoliophyta]
  • A61K8/9794—Liliopsida [monocotyledons]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/31—Hydrocarbons
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
  • A61K8/42—Amides
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
  • A61K8/73—Polysaccharides
  • A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
  • A61Q19/08—Anti-ageing preparations

Definitions

• the present invention relates to the use of at least one fatty acid chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof as a repellent active principle and / or for controlling reproduction of brachycera.
• the present invention also relates to the use of at least one fatty acid, in particular volatile, chosen from the group comprising propionic acid, butyric acid as a repellent active principle and / or for controlling the reproduction of brachycera.
• the present invention also relates to the use of a composition, in particular phytosanitary, comprising at least one fatty acid chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof as principle. active repellent and / or to control the reproduction of brachycera.
• the present invention also relates to the use of a composition, in particular phytosanitary, comprising at least one fatty acid, in particular volatile, chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof. here as a repellent active principle and / or to control the reproduction of brachycera.
• references in square brackets ([]) refer to the list of references presented at the end of the text.
• insects in agriculture are considered insect pests. They are in particular insects causing and / or causing damage and / or deterioration of crops. All agricultural crops, for example cereals, fruit, vegetables can be affected by pests. Insect pests are generally specific to a crop and / or plant type and can affect crops and / or plants differently. In other words, it is common for insect pests to be specific to a crop / plant and the effect on the crop / plant to be specific.
• insect pests can have an effect on the fruit of the plant, for example by its diet, and / or affect the "health" of the plant, for example by conveying pathologies, for example viruses, fungi. , etc., which may affect the growth and / or the production of fruits / flowers etc., of the plant / crop.
• pathologies for example viruses, fungi. , etc., which may affect the growth and / or the production of fruits / flowers etc., of the plant / crop.
• insect pests can alter the leaves, stems, buds, bark, wood, roots, flowers, fruits, seeds, at all stages of their development.
• insect pests can also affect products / fruits and / or any other item from crops.
• the object of the present invention is precisely to meet these needs and drawbacks by providing at least one fatty acid chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof for use as an active repellent principle. and / or to control the reproduction of brachycera.
• non-insecticidal compounds namely volatile fatty acids according to the invention
• advantageously have a repellent effect against brachycera
• the present invention in particular the use of the volatile fatty acids according to the invention, has a repellent effect against brachycera, advantageously by olfactory perception, in particular by perception. olfactory of adults.
• the present invention in particular the use of volatile fatty acids according to the invention, has an effect, in particular on the behavior of the brachycera linked to their reproduction.
• the inventors are the first to have demonstrated, surprisingly and unexpectedly, that the use according to the invention, in particular the use of fatty acids according to the invention, has an effect on the behavior of insect pests, in particular by the olfactory perception of these molecules.
• the inventors are the first to have demonstrated, surprisingly and unexpectedly, that the use according to the invention, in particular the use of fatty acids, in particular volatile, according to the invention, has an effect. on the behavior of pest insects, preferably adult insects, in particular by the olfactory perception of these molecules, without effect on the lethality of pest insects.
• the inventors have demonstrated that the present invention has no insecticidal effect on insect pests.
• the inventors have surprisingly demonstrated that the use of fatty acids according to the invention has a repellent effect, makes it possible to reduce or even abolish the display of insects and can induce anesthesia of the insects.
• the inventors have advantageously demonstrated that the use according to the invention, in particular the use of fatty acids, in particular volatile, according to the invention can induce a reversible anesthesia of the brachycera, advantageously allowing, unlike known insecticides, not to be toxic to brachycera.
• the present invention can advantageously induce a reversible anesthesia corresponding to a transient anesthesia not inducing toxicity on the adult brachycera.
• the inventors have also surprisingly demonstrated that the present invention, for example by reducing the display, advantageously makes it possible to reduce and / or prevent the proliferation of pest insects.
• the present invention advantageously makes it possible to reduce and / or prevent the proliferation of pest insects without affecting their role in the ecosystem apart from the crops to be protected.
• the inventors have also surprisingly demonstrated that the present invention advantageously makes it possible to reduce and / or prevent the reproduction and / or copulation of pest insects.
• the present invention has an effect on insect pests by smell perception, and advantageously makes it possible to reduce and / or eliminate the detection by insect pests of attracting olfactory signals, for example inducing and / or likely to stimulate the display preceding the mating of the insects.
• the present invention advantageously makes it possible to alter the detection by insect pests of attractive olfactory signals, for example between male and female.
• the present invention by the olfactory perception, advantageously makes it possible to reduce and / or inhibit the display and copulation of pest insects, behaviors upstream of the selective laying of said pest insects, by example on plants and / or crops, for example whose offspring feeds, without killing these insects and therefore protects their importance for the ecosystem outside, in particular the plants and / or crops to be protected.
• the invention uses fatty acids and / or derivatives thereof, which are compounds and / or molecules that are non-toxic to the environment, respectful of ecosystems and without known harmful effects on human and animal health.
• the present invention relates to the use of at least one fatty acid chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof as a repellent active principle and / or for controlling reproduction. of brachycera.
• a subject of the present invention is also the use of at least one fatty acid, which is advantageously volatile, chosen from the group comprising propionic acid and butyric acid as a repellent active principle and / or for controlling the reproduction of brachycera.
• a subject of the present invention is also the use of at least two fatty acids chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof as repellent active ingredients and / or for controlling the reproduction of brachycera.
• a subject of the present invention is also the use of propionic acid and butyric acid as active ingredients, which are advantageously volatile, repellents and / or for controlling the reproduction of brachycera.
• derivative denotes a chemical compound or a molecule made from a parent compound by one or more chemical reactions.
• salt denotes salts suitable for human or veterinary pharmaceutical use without toxicity, irritation, allergic response or other deleterious effect unsuitable for medicinal use and present a reasonable benefit / risk ratio.
• salt also designates salts suitable for phytosanitary use without toxicity, irritation, allergic response or other deleterious effect unsuitable for use, for example in agriculture.
• the term “repellent” means a removal and / or a repelling of the brachycera of plants and / or of plants and / or of fruits and / or of all or part of plants and / or of plants and / or of mammals. .
• the term “repellent” also means an inhibition of the attractiveness of brachycera for plants and / or plants and / or fruits and / or all or part of plants and / or plants and / or mammals.
• the term “repellent” also designates a disinterest of the brachycera for an environment and / or space in which one or more volatile odorous molecule (s) is (are) present.
• control of reproduction means an inhibition and / or a stabilization of the reproduction of brachycera in particular by inhibition of the display and / or a decrease in the display, and / or an inhibition of the flight in flocks and / or an inhibition of the supply, by the male, of a prey to the female and / or an inhibition of the formation by the male of a cocoon.
• control of reproduction is meant a reduction and / or inhibition of the display and / or copulation of brachycera, behaviors, preferably adults, upstream of the laying, preferably selective, of said.
• brachycera in particular upstream of spawning, for example on plants and / or plants, for example whose descendants, for example larvae, preferentially feed.
• brachycera the insects belonging to the family Muscidae, estridae, hippoboscidae, nyctéribiides, syrphides, tachinides, thaumatoxeneiids, thermitoxeneiids.
• brachycera insects belonging to the family of estrids, hippoboscidae, nyctéribiides, hoverflies, tachinids, thaumatoxeneiids, thermitoxeneiids.
• insects may be insects chosen from the group comprising insects of the genus Musca, Drosophila, Glossina, Calliphora, Auchmeromyia, Lucilia, Sarcophaga, Stomoxys, preferably, they may be insects selected from the group comprising insects of the genus Drosophila, Glossina, Calliphora, Auchmeromyia, Lucilia, Sarcophaga, Stomoxys. They may for example be insects belonging to the genus Drosophila chosen from the group comprising Drosophila suzukii, Drosophila melanogaster, preferably Drosophila suzukii.
• the insects when the insects are of the genus Musca, it can be any insect of the genus Musca except Musca domestica.
• the brachycera also include the insects belonging to the family of the trypetids also referred to as tephritids. They may, for example, be insects selected from the group comprising insects of the genus Bactrocera. They may, for example, be insects selected from the group comprising Bactrocera oleae.
• the brachycera can be chosen from insects belonging to the family of estrids, hippoboscidae, nyctéribiides, syrphides, tachinides, thaumatoxeneiidae, thermitoxeneiidae and / or Trypetidae.
• brachycera can be selected from the group consisting of Drosophila suzukii and Bactrocera oleae.
• propionic acid also designated propanoic
• propanoic is understood to mean a volatile C3 fatty acid, of gross formula C3H6O 2 , and / or of the following semi-structural formula (I):
• the propionic acid is a volatile fatty acid.
• the propionic acid is odorous.
• odorous compound is meant a compound which exhales an odor.
• derivative of propionic acid is meant any derivative and / or salts known to those skilled in the art. It may, for example, be a derivative chosen from the group comprising an ester of propionic acid, of an amine of propionic acid and / or a mixture thereof.
• the propionic acid ester can be any propionic acid ester known to those skilled in the art. It can be for example an ester of formula CH3-CH2-COOR1 (III) in which R 1 can be a C 1 to C 6 alkyl, for example a methyl, an ethyl, a butyl, a propyl, a pentyl, a hexyl, a group R11R12 in which Ru is an anion and R12 a cation selected from the group of alkali metals comprising lithium, sodium, potassium, rubidium and cesium.
• R 1 can be a C 1 to C 6 alkyl, for example a methyl, an ethyl, a butyl, a propyl, a pentyl, a hexyl, a group R11R12 in which Ru is an anion and R12 a cation selected from the group of alkali metals comprising lithium, sodium, potassium, rubidium and cesium
• the propionic acid ester can be selected from the group consisting of ethyl propanoate, propyl propanoate, butyl propanoate, isoamyl propanoate, isobutyl propanoate and / or dpropanoate. isopropyl.
• the ester of propionic acid is ethyl propanoate.
• the priopionic acid amine can be any propionic acid amine known to those skilled in the art.
• priopionic acid amine can be selected from the group consisting of propylamine, dipropylamine, tripropylamine, isopropylamine, diisopropylamine, triisopropylamine.
• ester when the ester is a derivative of propionic acid, preferably ethyl propanoate or ethyl butyrate, it has a more pleasant fragrance for humans than the acid.
• the propionic acid and / or derivative thereof can be at a concentration of 0.1 to 30% by volume (v / v).
• the concentration of propionic acid and / or a derivative thereof this can be adapted according to the brachycera.
• the propionic acid and / or derivative thereof can be at a concentration of 0.5% to 1%, 1% to 2% or 2% to 30% by volume (v / v).
• the concentration of propanoic acid can be equal to 0.5%, 1%, 2%, 4%, 5% by volume (v / v).
• the concentration of the propionic acid derivative can range from 2% to 30% by volume (v / v).
• the concentration of ethyl propanoate can be 10% by volume (v / v).
• butyric acid also referred to as butanoic acid, is meant a volatile C4 fatty acid, of gross formula C4H8O2, and / or of the following semi-developed formula (II):
• butyric acid is a volatile fatty acid.
• the butyric acid is odorous.
• derivative of butyric acid is meant any derivative and / or salts known to those skilled in the art. It may, for example, be a derivative chosen from the group comprising an ester of butyric acid and / or a salt thereof.
• the butyric acid ester can be any butyric acid ester known to those skilled in the art. It can be for example an ester of formula CH3- (CH2) 2-COOR2 (NI) in which R2 can be a C1 to C6 alkyl, for example a methyl, an ethyl, a butyl, a propyl, a pentyl, a hexyl, a group R11R12 in which Ru is an anion and R12 a cation selected from the group of alkali metals comprising lithium, sodium, potassium, rubidium and cesium.
• R2 can be a C1 to C6 alkyl, for example a methyl, an ethyl, a butyl, a propyl, a pentyl, a hexyl, a group R11R12 in which Ru is an anion and R12 a cation selected from the group of alkali metals comprising lithium, sodium, potassium, rubidium and
• the butyric acid ester can be chosen from the group comprising ethyl butyrate, propyl butyrate, butyl butyrate, isoamyl butyrate, isobutyl butyrate and / or isopropyl butyrate.
• butyric acid and / or derivative thereof may be at a concentration of 0.1 to 3% by volume (v / v).
• the concentration of butyric acid and / or derivative thereof can be adjusted depending on the brachycer.
• butyric acid and / or derivative thereof may be at a concentration of 0.5% to 3% by volume (v / v).
• the concentration of butyric acid can be equal to 0.5%, 1%, 2% by volume (v / v).
• the concentration of the butyric acid derivative can advantageously be equal to 2% by volume (v / v).
• the concentration of butyric acid derivative can be from 2% to 3% by volume (v / v). For example when the derivative is ethyl butyrate the concentration can be greater than or equal at 2% by volume (v / v).
• the concentration of propionic acid and / or of the derivative can be between 0.1 and 0.5% by volume (v / v); and the concentration of butyric acid and / or the derivative may be between 0.1% and 0.5% by volume (v / v).
• a subject of the present invention is also a composition comprising at least one fatty acid chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof and an acceptable carrier.
• a subject of the present invention is also a composition comprising at least one fatty acid, which is advantageously volatile, chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof and an acceptable support.
• a subject of the present invention is also a composition comprising at least one fatty acid, advantageously volatile, chosen from the group comprising propionic acid, butyric acid and an acceptable support.
• a subject of the present invention is also a composition comprising propionic acid and butyric acid and an acceptable carrier.
• a subject of the present invention is also a composition comprising propionic acid and butyric acid.
• a subject of the present invention is also the use of a composition comprising at least one fatty acid chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof and an acceptable carrier as a repellant and / or to control the reproduction of brachycera.
• a subject of the present invention is also the use of a composition comprising at least one fatty acid, advantageously volatile, chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof and a support. acceptable as a repellant and / or to control the reproduction of brachycera.
• a subject of the present invention is also the use of a composition comprising propionic acid, butyric acid and an acceptable carrier as a repellent and / or for controlling the reproduction of brachycera.
• a subject of the present invention is also the use of a composition comprising propionic acid, butyric acid as a repellent and / or for controlling the reproduction of brachycera.
• the support can be any support known to those skilled in the art suitable for being mixed and / or combined with fatty acids in a composition. It can be for example an oil, for example a mineral oil, a vegetable oil and / or a mixture of these. It may be, for example, any mineral oil known to those skilled in the art that is suitable. for use in the pharmaceutical, cosmetic and / or phytosanitary field. It may, for example, be a mineral oil chosen from the group comprising paraffin oil and petroleum jelly. Preferably, the mineral oil is paraffin oil.
• the paraffin oil has no significant effect on the brachycera.
• the paraffin oil advantageously allows a constant release of the fatty acid (s) and / or derivative (s) with which it is mixed.
• the support is paraffin oil
• the vegetable oil can be any vegetable oil known to those skilled in the art suitable for use in the pharmaceutical, cosmetic and / or phytosanitary field.
• the composition can be a cosmetic or dermatological composition, a pharmaceutical composition and / or a phytosanitary composition.
• cosmetic composition is understood to mean any form of cosmetic composition known to those skilled in the art.
• the cosmetic composition according to the invention can comprise one or more cosmetically acceptable adjuvants and / or support known to those skilled in the art.
• a conventional adjuvants and / or cosmetically acceptable carrier would prove to be incompatible with the compounds according to the invention, for example by producing any undesirable biological effect and / or having an odor and / or an effect liable to mask the odor of the compounds according to the invention, or else by interacting deleteriously with any other component of the cosmetic composition, its use is envisaged as falling within the scope of the present invention.
• adjuvant chosen from agents of ester type, moisturizing agents, emollients, emulsifiers, surfactants, inorganic thickeners, agents. organic thickeners, associative or not, of water-soluble and fat-soluble organic sunscreens, mineral sunscreens, silicone compounds, perfumes, preservatives, ceramides, and pseudo-ceramides, vitamins and provitamins, proteins, sequestering agents, basifying agents, acidifying agents , reducing agents, oxidizing agents, mineral fillers, colorants or any other suitable adjuvant which may be cited in the INCI dictionary (International Nomenclature of Cosmetic Ingredients) published by the PCPC (Personal Care Products council).
• adjuvant chosen from agents of ester type, moisturizing agents, emollients, emulsifiers, surfactants, inorganic thickeners, agents. organic thickeners, associative or not, of water-soluble and fat-soluble organic sunscreens, mineral sunscreens, silicone compounds, perfumes, preservative
• the cosmetic or dermatological composition may, for example, be in any form known to a person skilled in the art which may be used in the cosmetics field. Except in the case where the formulation of the cosmetic or dermatological composition would be incompatible with the compounds according to the invention, for example by producing any undesirable biological effect and / or an odor and / or an effect capable of masking the odor of the compounds according to the invention and / or by interacting deleteriously with any other component of the cosmetic composition, its use is envisaged as falling within the scope of the present invention. Those skilled in the art, based on this general knowledge, will know how to adapt the formulation according to the envisaged application.
• the cosmetic composition can be obtained by any suitable process known to those skilled in the art for the manufacture of a cosmetic composition. It can be a mixture of surfactants in water.
• the term “pharmaceutical composition” is understood to mean any form of suitable pharmaceutical composition known to those skilled in the art.
• the pharmaceutical composition can be for example a topical solution, an orodispersible galenic form and / or an aerodispersible solution, for example a liquid solution, a spray.
• the pharmaceutical composition can be a pharmaceutical composition for topical or transdermal administration, for example chosen from the group comprising ointments, creams, gels, lotions, patches and foams.
• composition in the present, can be formulated and / or adapted according to its administration.
• the composition of the present invention can also comprise at least one other active ingredient, particularly another therapeutically active ingredient, for example for simultaneous, separate or staggered use depending on the galenic formulation used.
• the pharmaceutically acceptable compositions of the present invention may further comprise a pharmaceutically acceptable carrier, adjuvant or vehicle, which, as defined herein, includes any solvent, diluent or other liquid vehicle. , dispersion or suspension aid, surfactant, isotonic agent, thickening or emulsifying agent, preservative, solid binder, lubricant and the like, adapted to the particular dosage form desired.
• a pharmaceutically acceptable carrier adjuvant or vehicle
• a pharmaceutically acceptable carrier includes any solvent, diluent or other liquid vehicle.
• dispersion or suspension aid includes any solvent, diluent or other liquid vehicle.
• surfactant isotonic agent
• thickening or emulsifying agent thickening or emulsifying agent
• preservative solid binder
• lubricant lubricant
• materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, ion exchangers, buffering substances, water, salts or electrolytes of waxes, sugars; starches, excipients, oils, glycols, esters, and other compatible non-toxic lubricants, as well as coloring agents, mold release agents, coating agents, sweetening agents, flavors, preservatives and antioxidants may also be present in the composition, depending on the judgment of the galenist.
• the fatty acid chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof and / or the composition comprising at least one fatty acid chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof can be applied by any suitable means known to those skilled in the art.
• it can be an application by means of a diffuser, a spray, a sprayer, an impregnated support.
• the means of application can be adapted depending on the composition used.
• a person skilled in the art by virtue of his general knowledge, will know how to adapt the means of application to the form of the composition used.
• a subject of the present invention is also a process for the protection of at least one biotic and / or abiotic surface against brachycera comprising the application to said surface of at least one fatty acid chosen from the group comprising propionic acid, butyric acid and / or a derivative thereof or a composition comprising at least one fatty acid selected from the group consisting of propionic acid, butyric acid and / or a derivative thereof.
• a subject of the present invention is also an ex-vivo process for the protection of at least one biotic and / or abiotic surface against brachycera comprising the application to said surface of at least one fatty acid chosen from the group comprising propionic acid, butyric acid or a composition comprising propionic acid and / or butyric acid.
• a subject of the present invention is also the ex-vivo use of propionic acid and butyric acid and or of a composition comprising propionic acid and butyric acid for the protection of at least one biotic and / or abiotic surface with respect to brachycera
• Propionic acid and / or a derivative thereof is as defined above.
• Butyric acid and / or a derivative thereof is as defined above.
• composition is as defined above.
• the present by protection of a surface is understood to mean the absence of laying on the surface, in particular by the reduction and / or inhibition of the display and / or copulation of brachycera, behaviors upstream of the laying of said brachycera and / or inhibition of the attractiveness and / or disinterest of brachycera for a surface present in the environment and / or space in which one or more volatile odorous molecule (s) is (are) present.
• surface any surface known to those skilled in the art. It can be, for example, a biotic or abiotic surface.
• biotic surface any biotic surface known to those skilled in the art. It may for example be a biotic surface chosen from the group comprising the skin, mucous membranes, hair, integuments, eyes, feathers. In the present the biotic surface can be a healthy surface and / or one having at least one lesion and / or an infection. It may for example be a biotic surface comprising a wound, an infected wound, a wound in the process of healing, a sutured wound, a scar, a burn.
• the biotic surface can be a surface of all or part of plants and / or plants. It can be, for example, the surface of leaves, fruits, flowers, petals, pistils, stems, branches, trunks. Preferably, it can be the fruit surface.
• abiotic surface any abiotic surface known to those skilled in the art. It can also be any device surface known to those skilled in the art. This can be, for example, the surface of a medical device, any surface present in rooms / rooms for medical emergencies, medical treatment, operation, operating theaters, rooms / rooms of care units intensive care units (UTI), rooms / rooms of infectious disease units. It can also be any surface of laboratory rooms / rooms, biological laboratory, biological analysis laboratory, incubation room and other enclosed volume. This can be, for example, the building surface, for example housing, storage and / or agriculture. This can be, for example, the surface of roofs, walls, windows, gutters.
• the abiotic surface can be formed by any material known to those skilled in the art. It may for example be a biocompatible material or not.
• the abiotic surface can be for example a metallic surface, a surface formed by an alloy, a polymeric surface.
• the metal can be any metal known to those skilled in the art, for example a metal chosen from the group comprising titanium, copper, iron, aluminum, nickel, tungsten, silver, l 'gold, palladium, vanadium, molybdenum.
• the alloy can be any alloy known to those skilled in the art, for example an alloy chosen from the group comprising steel, brass, copper-nickel, copper-palladium, silver-gold, silver-palladium, molybdenum-vanadium, molybdenum-tungsten.
• the polymer can be any polymer known to those skilled in the art capable of constituting and / or of covering a surface. It may for example be a polymer chosen from the group comprising polytetrafluoroethylene (PTFE), polysiloxanes, polyurethanes, functionalized polymers.
• PTFE polytetrafluoroethylene
• the application can be carried out by any means known to those skilled in the art.
• the application can be carried out with a diffuser, a sprayer, a pressurized diffuser.
• a diffuser e.g., a diffuser, a sprayer, a pressurized diffuser.
• the person skilled in the art by virtue of his general knowledge, will know how to adapt and / or choose the means of application according to the surface.
• the fatty acids according to the invention and / or the composition according to the invention are / is applied to an abiotic surface, they make it possible to prevent the appearance of brachycera near these surfaces, in particular, they make it possible to push back and / or move the brachycera away from said surfaces.
• a subject of the present invention is also a kit or a kit containing at least one fatty acid chosen from propionic acid and / or a derivative thereof, butyric acid and / or a derivative thereof, and / or a derivative thereof, and / or a derivative thereof. or a composition comprising at least one fatty acid chosen from propionic acid and / or a derivative thereof, butyric acid and / or a derivative thereof, and a support comprising instructions for use.
• Propionic acid and / or a derivative thereof is as defined above.
• Butyric acid and / or a derivative thereof is as defined above.
• composition is as defined above.
• the concentrations of propionic acid and / or butyric acid and / or derivative thereof are expressed as a percentage, unless otherwise mentioned, that is to say by volume propionic acid and / or butyric acid and / or derivative thereof relative to the total volume of the composition (v / v).
• FIG. 1 is a bar diagram representing the measurement of the proportion in percentage of display of Drosophila suzukii males with respect to females (ordered), in the absence or in the presence of a composition comprising 0.1%, 0.5% and 1% propionic acid (abscissa).
• ⁇ *** and ⁇ **** s jg n jf j en t a statistical difference between the different conditions presenting varying amounts of propionic acid and calculated by performing a Kruskall-Wallis test, followed by a post hoc Dunn multiple comparison test.
• FIG. 2 is a bar chart representing the measurement of the proportion in percentage of display of Drosophila suzukii males with respect to females (ordered) in the absence or in the presence of a composition comprising 0.1%, 0 , 5% and 1% butyric acid (abscissa).
• ⁇ *** and ⁇ **** s jg n jf j in f a statistical difference between the different conditions presenting varying amounts of butyric acid and calculated by performing a Kruskall-Wallis test, followed by a post hoc Dunn multiple comparison test.
• Figt / re 3 is a bar diagram representing the measurement of the proportion in percentage of display of Drosophila suzukii males with respect to females (ordered) in the absence or in the presence of a composition comprising respectively 0.1 %, 0.5% and 1% propionic acid or 0.1%, 0.5% and 1% butyric acid or mixtures corresponding to 0.1%, 0.25% or 0.5% d propionic acid and butyric acid (abscissa).
• ( * ) means a statistical difference between the different conditions with varying amounts of fatty acids. The statistical test performed is a Kruskall-Wallis test, followed by a post-hoc Dunn multiple comparison test.
• FIG. 4 is a bar diagram representing the measurement of the proportion in percentage of copulation of Drosophila suzukii (ordinates) in the absence or in the presence of a composition comprising respectively 0.1%, 0.5% and 1% propionic acid (abscissa).
• ( * ) means a statistical difference between the different conditions with varying amounts of propionic acid. The statistical test performed is a Kruskall-Wallis test, followed by a post-hoc Dunn test with multiple comparisons.
• FIG. 5 is a bar chart representing the measurement of the proportion in percentage of copulation of Drosophila suzukii (ordinates) in the absence or in the presence of a composition comprising respectively 0.1%, 0.5% and 1% butyric acid (abscissa).
• ( * ) means a statistical difference between the different conditions with varying amounts of butyric acid. The statistical test performed is a Kruskall-Wallis test, followed by a post-hoc Dunn test with multiple comparisons.
• FIG. 6 is a bar graph showing the time elapsed leading to anesthesia of male or female Drosophila suzukii (abscissa) in minutes and seconds (ordinate) in the presence of a composition comprising 2% propionic acid.
• a Mann-Whitney test (nonparametric t test) does not reveal a time-related difference leading to anesthesia of the flies according to their sex.
• - Figure 7 is a bar chart representing the time elapsed leading to anesthesia of male or female Drosophila suzukii (abscissa) in minutes and seconds (ordinate) in the presence of a composition comprising 2% butyric acid.
• a Mann-Whitney test (nonparametric t test) does not reveal a time-related difference leading to anesthesia of the flies according to their sex.
• - Figure 8 is a photograph showing part of the interior of a transparent 1250 cm 3 test dish in which 2% propionic acid has been placed in a Petri dish covered with gauze (in outside the frame of the image shown).
• the black dots in the figure correspond to Drosophila suzukii flies.
• the arrow indicates a Drosophila suzukii fly on its back, in anesthesia position.
• FIG. 9 is a photograph showing part of the interior of a transparent 1250 cm 3 test dish in which 2% butyric acid has been placed in a Petri dish covered with gauze (in outside the frame of the image shown).
• the black dots in the figure correspond to Drosophila suzukii flies.
• the arrow indicates a Drosophila suzukii fly on its back, in anesthesia position.
• FIG. 10 is a bar graph representing the measure of the percentage of display of Drosophila melanogaster
• FIG. 11 is a bar graph showing the measure of the percentage parade proportion of Drosophila melanogaster
• FIG. 12 is a bar chart representing the time elapsed leading to anesthesia of male or female Drosophila melanogaster (abscissa) in minutes and seconds (ordinate) in the presence of a composition comprising 2% propionic acid.
• **** means a statistical difference between the different conditions presenting varying amounts of propionic acid and calculated by performing a Mann-Whitney test (non-parametric test).
• FIG 13 is a bar chart representing the time elapsed leading to anesthesia of male or female Drosophila melanogaster (abscissa) in minutes and seconds (ordinate) in the presence of a composition comprising 2% butyric acid.
• abcissa the time elapsed leading to anesthesia of male or female Drosophila melanogaster
• ordinate the time elapsed leading to anesthesia of male or female Drosophila melanogaster
• a composition comprising 2% butyric acid.
• *** means a statistical difference between the different conditions exhibiting varying amounts of propionic acid and calculated by performing a Mann-Whitney test (non-parametric test).
• FIG. 14 corresponds to three photographs of the same test box ( Figure 14A as a whole and partly top view ( Figure 14B and Figure 14C)) used for the study of repulsion and / or anesthesia behavior remote from D. melanogaster and D. suzukii.
• This transparent dish with a volume of 1250 cm 3 2 Petri dishes are placed: one, covered with gauze (visible to the right of the image showing the test dish as a whole), contained l propionic acid at 2%, 6% or 20% and the other contained synthetic food (visible on the left of the image).
• the black dots correspond to Drosophila melanogaster flies.
• FIG. 15 is a diagram showing one of the six cylindrical cells of the behavioral device used for the study of the display of D. melanogaster. At the base of the cylinder is deposited a filter paper soaked in an odor, the central part maintains a veil on which the tested flies move, this cylinder is finally closed. For D. melanogaster display tests, a red light illuminates the device.
• FIG. 16 is a diagram representing a behavior device used for the study of the display of D. suzukii. It is similar to that used for D. melanogaster but is illuminated by white light.
• FIG. 17 is a bar chart representing the time elapsed leading to anesthesia of Drosophila suzukii females in minutes and seconds (ordinate) in the presence of a composition comprising 2% butyric acid, 2% propionic acid, 5% ethyl propanoate, 10% ethyl propanoate or 30% ethyl propanoate (abscissa).
• FIG. 18 is a bar graph showing the measurement of the percentage proportion of male display versus female Bactrocera oleae 3 to 6 days old (ordered) in the absence (odorless or odorless). solvent) or in the presence of a composition comprising respectively 2%, 4% and 5% propanoic acid (abscissa). In the figure, the figures represent the number of pairs observed.
• FIG. 19 is a bar graph representing the measurement of the proportion in percentage of display of males vis-à-vis females of Bactrocera oleae flies aged 13 to 19 days (ordered) in the absence (odorless or solvent ) or in the presence of a composition comprising respectively 2% and 5% propanoic acid (abscissa). In the figure, the figures represent the number of pairs observed.
• FIG. 20 is a bar chart representing the time (in minutes or seconds) required for male and female Drosophila melanogaster flies (abscissa) to wake up from anesthesia (ordinate) induced by exposure to a composition comprising respectively 2% propanoic acid (left side on the x-axis) or 2% butyric acid (right side on the y-axis).
• the figures indicate the number of individuals tested, respectively 20 males and 30 females previously exposed to 2% propanoic acid, and 6 males and 7 females previously exposed to 2% butyric acid.
• Example 1 Effect of propionic acid and / or butyric acid on the reproduction of brachycera
• the brachycera used were the (musk) flies Drosophila melanogaster and Drosophila suzukii.
• a study of courtship and copulation was performed.
• Naive males were isolated in tubes, individually, as they emerged from their puparium. Females were chosen virgin and stored in tubes of 10 individuals. These tubes were placed in incubators for which a temperature of 25 ° C is kept constant. A day-night cycle, 12h - 12h has been applied. The parry tests were carried out in the morning, between 1 and 3 hours after the start of the day cycle. These tests were carried out in devices made up of 6 cylindrical, transparent and closed cells (see figure 15) with a volume of 1, 52 or cm 3 . At their base a disc of filter paper (Whatman, filter paper 42) 6 mm in diameter is deposited.
• Said paper is soaked with 10 ⁇ l of a composition comprising propionic acid and / or butyric acid diluted in paraffin oil (solvent).
• the different compositions used respectively comprised 0.5% or 1% propionic acid, 0.5% or 1% butyric acid. For each concentration, the number of pairs of flies tested were 23, 23, 19 and 19 respectively.
• the fruit flies were physically isolated from the source of the odor (said paper) by a veil which prevents any direct contact, the female was decapitated but remained alive. Also, the device was illuminated with red light (far red wavelength between 625 and 630 nm), so as to eliminate any behavior related to visual perception, included in D. melanogaster between 300 and 550 nm) ). So the observed behavior depends only on the olfactory perception by the male of the compound introduced into the device at the time of its assembly.
• the behavior was filmed with a Sony HDR-XR550 camera for 10 minutes. Each film was recorded and kept for later analysis.
• a display proportion expressed as a percentage was then calculated and represents the time during which the male displays the female during the 10 minutes of recording of the test.
• the display of Drosophila melanogaster is broken down into recognizable stereotypical events and precisely described in the scientific literature (Greenspan & Ferveur, Annu. Rev. Genet. 2000
• a statistical test Mann-Whitney test (nonparametric test, GraphPad, Prism8)) in order to evaluate the influence on the behavior of the composition impregnated on the paper was carried out; the latter involving a release of volatile compounds and an odor perceived by the Drosophila.
• a negative control was performed and corresponded by soaking the filter paper with a solution of paraffin oil alone (the solvent) in order to verify the absence of effect on the flies.
• Drosophila (Drosophila suzukii) aged 3 to 9 days are used for the display tests according to the method described above.
• Naive males were isolated in tubes, individually, as they emerged from their puparium. Females were chosen virgin and stored in tubes of 10 individuals. These tubes were placed tilted in incubators for which a temperature of 25 ° C was kept constant. A day-night cycle, 12h - 12h has been applied. The parry tests were carried out in the morning, between 1 and 3 hours after the start of the day cycle.
• FIG. 16 is a schematic representation of a cell of the device used.
• the paper was then soaked with 10mI of a composition of 0.1%, 0.5% or 1% propionic acid, or 0.1%, 0.5% or 1% butyric acid or d 'a mixture comprising 0.1% propionic acid and 0.1% butyric acid, 0.25% propionic acid and 0.25% butyric acid or 0.5% propionic acid and 0 , 5% butyric acid.
• a gauze (veil) physically isolates the fruit flies from the smell. 6 pairs (one per alveolus) were observed independently. Each pair consisted of a male and an intact female, each from the tubes mentioned above.
• the behavior was filmed with a Sony HDR-XR550 camera for 10 minutes, under white light. Each film was recorded and then analyzed. For each concentration of the compositions used, the number of pairs of flies were 33, 39, 27, 38, 33, 27, 12, 6 and 12, respectively.
• a negative control was carried out comprising only the solvent, namely paraffin oil, in order to verify the absence of effect of the solvent on the flies.
• a display percentage was calculated and represents the time during which the male displays the female during the 10 minutes of observation (recording).
• the display of Drosophila suzukii is broken down into recognizable stereotypical events and precisely described in the scientific literature (Revadi S et al., Insects 2015 [4]).
• Statistical tests namely a Kruskal-Wallis test followed by a post-hoc Dunn test with multiple comparisons, were carried out (GraphPad, Prism6) in order to assess the influence on the behavior of the soaked composition on the paper; the latter involving a release of volatile compounds and an odor perceived by the Drosophila.
• Figures 1 to 3 show the results obtained with Drosophila suzukii flies as a function of the presence or absence of propionic acid, butyric acid or a mixture of propionic acid and butyric acid, respectively.
• Figures 10 and 11 show the results obtained with Drosophila melanogaster flies as a function of the presence or absence of propionic acid or butyric acid, respectively.
• the proportion of display of males with respect to females was significantly modified ( * ) in the presence of the composition comprising 1% of propionic acid relative to the solvent alone.
• the display was also significantly reduced ( * ) in the presence of 1% propionic acid in the solvent compared to 0.5% propionic acid in the solvent.
• the proportion of display of males with respect to females was significantly reduced ( * ) in the presence of a composition comprising 0.5% butyric acid compared to the display in the solvent alone.
• the display was also significantly reduced ( *** ) in the presence of a composition comprising 1% butyric acid compared to the display in the solvent alone.
• Figures 4 and 5 show the results obtained with Drosophila suzukii flies as a function of the presence or absence of propionic acid, butyric acid or a mixture of propionic acid and butyric acid, respectively.
• the coupling proportion was reduced by 58% in the presence of a composition comprising 0.1% propionic acid diluted in the solvent.
• a composition comprising 0.5% propionic acid (( * ) vs. solvent alone) or 1% acid propionic (( * ) vs. solvent alone) the coupling proportion is zero, no coupling was observed.
• the coupling proportion was decreased by 70.55% in the presence of a composition comprising 0.1% butyric acid.
• a composition comprising 0.5% butyric acid coupling was decreased by 83.03% (from 17.86% to 3.03%), and in the presence of a composition comprising 1% d butyric acid (( * ) vs. solvent alone), the coupling proportion is zero, no coupling was observed.
• compositions according to the invention make it possible both to reduce the display of males vis-à-vis females and also to reduce or even eliminate copulation of brachycera.
• This example also demonstrates that propionic acid, butyric acid and / or a derivative thereof are useful as an active ingredient to control the reproduction of brachycera.
• Example 2 Effect of propionic acid and / or butyric acid and / or a derivative of propionic acid on the reproduction of brachycera and / or as repellent active ingredients
• the brachycera used were the flies Drosophila melanogaster and Drosophila suzukii.
• Drosophila (Drosophila melanogaster and Drosophila suzukii) were as described in Example 1, however, D. melanogaster females were not decapitated.
• the devices used were similar to the display study devices (example 1 above), except the volume of the space in which the flies were introduced which was 45.24 or 22.62 cm 3 .
• 9 discs of filter paper (Whatman, filter paper 42) each soaked with 10 ⁇ l of a composition comprising butyric acid and / or propionic acid and / or a derivative of propionic acid diluted in paraffin oil were deposited in the device.
• the different compositions used respectively comprised 2% propionic acid or 2% butyric acid or 5% ethyl propanoate or 10% ethyl propanoate or 30% ethyl propanoate.
• the number of flies were respectively for Drosophila melanogaster 30 and 24 males, 30 and 11 females; and for Drosophila suzukii of 24, 16 and 4 males, 28, 26, 6, 5 and 4 females.
• a gauze was placed above the said papers. The fruit flies could move freely on this gauze.
• the amount of compound, butyric acid or propionic acid or ethyl propanoate (odor) volatilized in the behavior chamber was equivalent to that of the device used for the parry.
• the flies were never in direct contact with the composition (no taste perception), only with the volatile particles (olfactory perception).
• the flies tested were introduced simultaneously in groups into the enclosure. Their behavior was filmed for 10 minutes and a time required to lead to anesthesia was calculated over this period, depending on the concentration of butyric acid. or propionic acid or ethyl propanoate used. Flies were considered anesthetized when they stopped moving and fell to the side.
• a Mann-Whitney test did not reveal a difference in the time elapsed before anesthesia of the males or females tested.
• the sensitivity of the Drosophila was evaluated over a period of from 0 min to 24 hours depending on the conditions (see results) in closed and transparent boxes of 1250 cm 3 .
• An odor source namely a composition comprising butyric acid or propionic acid in paraffin oil, was placed on filter paper at the bottom of a 50mm Petri dish.
• the Petri dish is placed in the test dish and covered with a gauze (sterile Securimed M8202 gauze pad) in order to prevent the flies from becoming stuck with the oily odorous compound.
• gauze sterile Securimed M8202 gauze pad
• the concentrations used were 2%, 6% or 20% propionic acid.
• test dish containing 5 to 6 grams of synthetic food used for the rearing of Drosophila in the laboratory, was placed in the test dish, namely 6.5% corn flour, 6.5% (v / v) yeast extract, 1% (v / v) agar-agar and 3% (v / v) of a 0.1% (v / v) solution of antifungal (Tegosept (Apex) ) in 95% ethanol.
• the latter provides the water and / or food that the flies needed during the test and eliminated a possibility of an event due to dehydration of the individuals tested.
• Figures 6 to 9 and Figure 17 show the results obtained with Drosophila suzukii flies as a function of the presence or absence of propionic acid or butyric acid or ethyl propanoate, respectively.
• Figure 8 is a photograph demonstrating the anesthesia of Drosophila suzukii flies after their introduction into a transparent 1250 cm 3 test dish, where propionic acid diluted to 2% in the solvent was placed in a covered Petri dish gauze to avoid direct contact. The individuals initially remained, preferably away from this odor, then the males and females were anesthetized within the hour which followed. The photograph shows the location of the flies and an individual on its back, in anesthesia position within one hour of its introduction into the box.
• Figure 9 is a photograph demonstrating the anesthesia of Drosophila suzukii flies after their introduction into a transparent 1250 cm 3 test dish, where butyric acid diluted to 2% in the solvent was placed in a covered Petri dish gauze to avoid direct contact.
• the flies present in the transparent test box remained away from the filter soaked in butyric acid, both males and females are anesthetized and die after 20 hours in this environment.
• the photograph shows the location of the flies and an individual on the back, in anesthesia position within one hour of its introduction into the box
• Figures 12 and 13 obtained in the devices described in A1, represent the results obtained with Drosophila melanogaster flies as a function respectively in particular of the presence or absence of propionic acid or butyric acid.
• Figure 17 obtained in the devices described in A1 represent the results obtained with female Drosophila suzukii flies in the presence of 2% butyric acid or 2% propionic acid or 5% ethyl propanoate or 10% propanoate ethyl or 30% ethyl propanoate.
• Figure 14 shows the results obtained with Drosophila Melanogaster flies.
• the flies In a box where propionic acid diluted to 2% or 6% in the solvent (paraffin oil), the flies are still alive after 24 hours. During this period, males and females display and copulate (Figure 14); taken out of the box they will have viable offspring.
• the individuals stand away from the filter paper soaked in the odorous composition and are then anesthetized within one hour of testing.
• Example 3 Effect of propionic acid and / or butyric acid and / or a derivative of propionic acid on the reproduction of brachycera and / or as repellent active ingredients
• the brachycera used were the Bactrocera oleae flies aged 3 to 6 days or 13 to 19 days respectively.
• Olive tree flies (Bactrocera olea) 3 to 6 days old or sexually mature (9 to 13 days old) were used for the male display tests against females (Bonelli G., J. Insect Behav. 2012 [6] and Ménagé Cindy, personal communication). Naive males were isolated in tubes, individually, as they emerged from their puparium. Females were chosen virgin and stored in tubes together. These tubes were placed tilted in incubators for which a temperature of 25 ° C was kept constant. A day-night cycle, 12h - 12h has been applied. The display tests were carried out in the morning, 1 hour before the end of the day cycle, when the males are most active (Bonelli G., J. Insect Behav. 2012 [6]).
• FIG. 16 is a schematic representation of a cell of the device used.
• the behavior was filmed with a Baesler acA1920-155um USB 3.0 camera for 10 minutes, under white light. Each film was recorded (Basler Video Recording Software V1.3) and then analyzed. For each concentration of the compositions used, the number of pairs of flies were respectively 2, 5, 2, 2, 2 and 6, 6, 4, 4.
• a negative control was carried out comprising only the solvent, namely paraffin oil, in order to verify the absence of effect of the solvent on the flies.
• a display percentage was calculated and represents the time during which the male displays the female during the 10 minutes of observation (recording).
• the different stages consist of wing movements (rapid “buzzing” and / or vertical vibrations), front, lateral movements, “dances” in rapid circles and towards the female (Benelli, J. Insect Beahv. 2012 [6] and Ménagé Cindy, personal communication).
• Figures 18 and 19 show the results obtained with Bactrocera olea flies as a function of the presence or absence of propionic acid and according to their age.
• compositions according to the invention make it possible to reduce the display of males vis-à-vis females.
• This example also demonstrates that propionic acid is useful as an active ingredient to control the reproduction of brachycera without insecticidal effect.
• this example clearly demonstrates that the presence of propionic acid advantageously allows a reduction and / or inhibition of the display and / or copulation of brachycera. This example also clearly demonstrates that the presence of propionic acid controls reproduction upstream of spawning.
• Example 4 Effect of suppression / reversible effect of propionic acid and / or butyric acid and / or a derivative of propionic acid on the anesthesia of brachycera
• the brachycera used were the Drosophila melanogaster flies.
• Said fruit flies (Drosophila melanogaster) were those described in Example 2.
• the devices used were those described for the study of anesthesia as described in Example 2 above.
• the conditions for applying propanoic acid or butyric acid were similar to those for the anesthesia test described in Example 2 above.
• the flies were placed in the presence of 2% propanoic acid or 2% butyric acid for 10 minutes.
• the total number of flies introduced and observed were 20 males and 30 females previously exposed to 2% propanoic acid and removed from this anesthetic environment, and 6 males and 7 females previously exposed to 2% propanoic acid. butyric acid and removed from this numbing environment.
• the behavior of the flies in the empty devices was observed for 25 minutes and filmed.
• the flies were considered to be awake from the anesthesia when they stood up on their legs and moved around the device.
• Figure 20 shows the results obtained with male and female Drosophila melanogaster flies respectively, following their exposure to 2% propanoic acid and / or 2% butyric acid respectively.
• the males wake up completely from the anesthesia after 23 minutes when they have previously been placed for 10 minutes in the presence of 2% propanoic acid, then removed from this device. odorous to place them in an empty device.
• Females wake up fully after 9 minutes when they have been previously placed for 10 minutes in the presence of 2% propanoic acid and then removed from this scent device to place them in an empty device.
• the males fully wake up from the anesthesia after 6 minutes when they have been previously placed for 10 minutes in the presence of 2% butyric acid, then removed from this device. odorous to place them in an empty device.
• Females wake up fully after 4.5 minutes when they have been previously placed for 10 minutes in the presence of 2% butyric acid and then removed from this scent device to place them in an empty device.
• an example of a composition according to the invention advantageously allows reversible anesthesia on the brachycera.
• this example clearly demonstrates that the use of an example of a composition according to the invention is, in particular by virtue of its reversible effect, non-toxic with respect to brachycera.

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