EP3978129A1 - Verfahren zur bereitstellung von selbstdetektion einer bedingung mit offener schaltung oder geschlossener schaltung in einer dielektrischen vorrichtung - Google Patents

Verfahren zur bereitstellung von selbstdetektion einer bedingung mit offener schaltung oder geschlossener schaltung in einer dielektrischen vorrichtung Download PDF

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Publication number
EP3978129A1
EP3978129A1 EP21199850.5A EP21199850A EP3978129A1 EP 3978129 A1 EP3978129 A1 EP 3978129A1 EP 21199850 A EP21199850 A EP 21199850A EP 3978129 A1 EP3978129 A1 EP 3978129A1
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EP
European Patent Office
Prior art keywords
voltage
detection
electrode
operational amplifier
terminal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP21199850.5A
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English (en)
French (fr)
Inventor
Hung-Yun Huang
Keng-Yuan Chen
Wei-Yang Hsu
Chung-Yao Chen
Yu-Fu Weng
Ting-Chieh Lu
Chun-Jen Lin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Century Technology Shenzhen Corp Ltd
Icare Diagnostics International Co Ltd
Original Assignee
Century Technology Shenzhen Corp Ltd
Icare Diagnostics International Co Ltd
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Priority claimed from CN202110744774.5A external-priority patent/CN114336510A/zh
Application filed by Century Technology Shenzhen Corp Ltd, Icare Diagnostics International Co Ltd filed Critical Century Technology Shenzhen Corp Ltd
Publication of EP3978129A1 publication Critical patent/EP3978129A1/de
Withdrawn legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces

Definitions

  • the subject matter herein generally relates to nucleic acid testing, and particular to a method for circuit self-detection of an electrowetting on dielectric device.
  • Coupled is defined as connected, whether directly or indirectly through intervening components, and is not necessarily limited to physical connections.
  • the connection can be such that the objects are permanently connected or releasably connected.
  • perpendicular “horizontal”, “left”, “right” are merely used for describing, but not being limited.
  • FIG. 1 illustrates one embodiment of a detection chip 10.
  • the detection ship 10 includes a chip casing 1, a channel 2, and a driving loop 3.
  • the channel 2 is disposed in the chip casing 1 and receives a droplet D with a sample of nucleic acid or other sample for testing.
  • the droplet D will undergo a nucleic acid amplification reaction in the channel 2.
  • the chip casing 1 includes a first cover 11, a spacer layer 12, and a second cover 13. Two opposite surfaces of the spacer layer 12 are respectively adjacent to the first cover 11 and the second cover 13. The first cover 11, the spacer layer 12, and the second cover 13 cooperatively form the channel 2.
  • the driving loop 3 drives the droplet D to move along a specified path for executing the nucleic acid amplification reaction.
  • the driving loop 3 includes some driving electrodes 31 disposed on a side surface of the first cover 11 adjacent to the channel 2, a first dielectric layer 33 disposed on a side of the driving electrodes 31 adjacent to the second cover 13, a detection electrode 32 disposed on a side surface of the second cover 13 adjacent to the channel 2, and a second dielectric layer 34 disposed on a side of the detection electrode 32 adjacent to the first cover 11.
  • the driving electrodes 31 and the detection electrode 32 are disposed on opposite sides of the channel 2. By powering on and powering off the driving electrode 31 and the detection electrode 32, the droplet D in the channel 2 is moved along the specified path.
  • the driving electrodes 31 in the driving loop 3 are arranged in a matrix.
  • a conductive layer disposed on a side surface of the second cover 13 adjacent to the channel 2 serves as the detection electrode 32.
  • the driving electrodes 31 are disposed on a side of the first cover 11 adjacent to the channel 2.
  • the driving electrodes 31 can be formed by a metal etching manner or by electroplating.
  • the driving loop 3 is a thin film transistor (TFT) driving loop.
  • TFT thin film transistor
  • EWOD electrowetting on dielectric
  • the droplet D moves along the specified path in the channel 2.
  • the TFTs enable a circuit between the driving electrode 31 and one of the detection electrodes 32 to be turned on or turned off, a voltage between the driving electrode 31 and the detection electrode 32 can be adjusted.
  • a wetting property between the first dielectric layer 33 and the second dielectric layer 34 can be adjusted for controlling the droplet D to move along the specified path.
  • the droplet D can move on the electrodes A-C.
  • a voltage is applied on the electrode B and the detection electrode 32, and a voltage applied to the electrode A and the detection electrode 32 is turned off.
  • the wetting property between the first dielectric layer 33 and the second dielectric layer 34 is changed, which causes a liquid-solid contact angle between the electrode A and the droplet D to increase, and a liquid-solid contact angle between the electrode B and the droplet D to decrease, thus the droplet D moves from the electrode A to the electrode B.
  • FIGS. 2 and 3 respectively show a first embodiment of a diagram and a circuit diagram of a dielectric wetting device 100 in a normal state.
  • the dielectric wetting device 100 includes the detection chip 10, a power input module 20, a switch module 30, a detection module 40, and a determination module 50.
  • the power input module 20 is electrically connected to the detection chip 10 through the switch module 30.
  • the power input module 20 is electrically connected to the driving electrodes 31 of the detection chip 10 through the switch module 30 and applies a power voltage V in to the driving electrodes 31.
  • the detection module 40 is electrically connected to the detection electrode 32.
  • the detection module 40 receives the detection voltage V out outputted by the detection electrode 32, and accumulates the detection voltage V out to obtain an accumulation voltage V p .
  • the detection module 40 includes a voltage accumulation circuit 41.
  • the voltage accumulation circuit 41 includes a first operational amplifier U 1 , a first capacitor C 1 , a first diode D 1 , a second diode D 2 , a second operational amplifier U 2 , a first resistor R 1 , a second resistor R 2 , and a first capacitor C 1 .
  • a positive terminal of the first operational amplifier U 1 is electrically connected to the detection electrode 32, and a negative terminal of the first operational amplifier U 1 is electrically connected to an anode electrode of the first diode D 1 and a terminal of the second resistor R2.
  • An output terminal of the first operational amplifier U 1 is electrically connected to an anode electrode of the second diode D 2 and a cathode electrode of the first diode D 1 .
  • a cathode electrode of the second diode D 2 is electrically connected to a terminal of the first resistor R 1
  • another terminal of the first resistor R 1 is electrically connected to a positive terminal of the second operational amplifier U 2 and a terminal of the first capacitor C 1 .
  • Another terminal of the first capacitor C 1 is grounded.
  • a negative terminal of the second operational amplifier U 2 is electrically connected to another terminal of the second resistor R 2 and an output terminal of the second operational amplifier U 2 .
  • the output terminal of the second operation amplifier U 2 serves as an output terminal of the detection module 40 for outputting the accumulated voltage V p of the detection voltage V out .
  • the determination module 50 is electrically connected to the detection module 40.
  • the determination module 50 receives the accumulated voltage V p , and compares the received accumulated voltage V p with the specified voltage V r for determining whether a short circuit state or an open circuit state exists in the detection chip 10. A position of the detection chip 10 in the short circuit state or in the open circuit state can also be confirmed.
  • the voltage accumulation circuit 41 can include the voltage accumulation circuit 41 (peak detector), not being limited.
  • the detection module 40 also can include other circuits, such as a filter circuit.
  • the first dielectric layer 33 and the second dielectric layer 34 are hydrophobic insulation layers, such as polytetrafluoroethylene coating.
  • the first dielectric layer 33 and the second dielectric layer 34 present an insulating and hydrophobic function, the droplet D is moved smoothly along the specified path, and fragmentation or breakage of the droplet is prevented while the droplet D is being moved.
  • FIG. 4 is a circuit diagram of the EWOD device 100 in one embodiment.
  • equivalent capacitors are formed in the driving loop 3 between the first dielectric layer 33, the second dielectric layer 34, and the channel 2 of the detection chip 10.
  • the first dielectric layer 33 forms a first dielectric capacitor C di-B in the driving loop 3.
  • the second dielectric layer 34 forms a second dielectric capacitor C di-T .
  • the channel 2 between the first dielectric layer 33 and the second dielectric layer 34 without silicone oil forms an equivalent air capacitor C air .
  • the capacitance of the equivalent air capacitor C air is changed according to a quantity of silicone oil in the channel 2 between the first dielectric layer 33 and the second dielectric layer 34.
  • each driving loop 3 formed by each driving electrode 31 the first dielectric capacitor C di-B , the air capacitor C air , and the second dielectric capacitor C di-T are electrically connected in series.
  • a terminal of the first dielectric capacitor C di-B away from the air capacitor C air is electrically connected to the corresponding driving electrode 31, and a terminal of the second dielectric capacitor C di-T away from the air capacitor C air is electrically connected to the detection electrode 32.
  • a first resistor (R BA , R BB , R BC ) (equivalent resistor) and a second capacitor (C BA , C BB , C BC ) (equivalent capacitor) are formed based on the wire connecting the switch unit 4 and the driving electrodes 31.
  • the first resistor (R BA , R BB , R BC ) and the second capacitor (C BA , C BB , C BC ) are electrically connected in series.
  • the power voltage V in outputted by the power input module 20 is a continuous square pulsed voltage.
  • the detection voltage V out is also a continuous square pulsed voltage.
  • the switch module 30, by a controller (not shown), can turn on one of the driving electrodes 31 and the driving electrodes 31 for sequential detection, a position of the loop between the driving electrode 31 and the detection electrode 32 in the open circuit state or in the short circuit state can be accurately confirmed.
  • the accumulated voltage V p of the driving loop 3 in a normal state firstly needs to be detected for serving as the specified voltage V r .
  • the accumulated voltage V p of the driving loop 3 is equal to the specified voltage V r .
  • the circuit detection principle of the EWOD device 100 will be described.
  • FIG. 4 shows the circuit diagram of the EWOD device 100
  • FIG. 5 shows waveforms of voltages of the EWOD device 100.
  • the electrode A and the detection electrode 32 form a driving loop 3.
  • the continuous square pulsed voltage of the power input module 20 is provided to the electrode A through the equivalent resistor RBA, the electrode A couples with the detection electrode 32, and the detection electrode 32 outputs the detection voltage V out (coupled voltage) to the voltage accumulation circuit 41 (peak detector) through the equivalent resistor between the detection electrode 32 and the detection module 40.
  • the voltage accumulation circuit 41 accumulates the detection voltage V out to obtain the accumulated voltage V p , and outputs the accumulated voltage V p to the determination module 50.
  • the determination module 50 computes the difference between the accumulated voltage V p and the specified voltage V r to determine whether the EWOD device 100 is in a normal state.
  • FIG. 6 shows the circuit diagram of the EWOD device 100 in the open circuit state.
  • the power voltage V in of the power input module 20 (the continuous square pulsed voltage as shown in FIG. 7 ) is provided to a driving loop 3 with the specified driving electrode 31 through the switch module 30.
  • the driving loop 3 is in the open circuit state
  • the power voltage V in of the power module 20 is not provided to the specified electrode 31 through the switch module 30 and the detection electrode 32, and the detection electrode 32 does not output the detection voltage V out .
  • the voltage accumulation circuit 41 (peak detector) of the detection module 40 does not receive the detection voltage V out , and does not accumulate the detection voltage V out to obtain the accumulated voltage V p . Therefore, the determination module 50 can easily determine that the driving loop 3 of the specified driving electrode 31 is in the open circuit state.
  • the voltage difference ⁇ V 1 is a difference between the accumulated voltage V p and the specified voltage V r .
  • the voltage difference ⁇ V 1 becomes larger.
  • the power voltage V in of the power input module 20 (the continuous square pulsed voltage as shown in FIG. 9 ) is provided to a driving loop 3 with the specified driving electrode 31 through the switch module 30.
  • the detection electrode 32 outputs the detection voltage V out
  • the voltage accumulation circuit 41 (peak detector) of the detection module 40 accumulates the detection voltage V out to obtain the accumulated voltage V p .
  • the detection module 40 outputs the accumulated voltage V p to the determination module 50 for comparison.
  • the power voltage V in of the power module 20 is not provided to the circuit through the switch module 30, different wires between the specified driving electrode 31 are electrically connected with each other, the resistance of one of the resistors R BA -R BB being driven is increased, and the accumulated voltage V p decreases.
  • the voltage difference ⁇ V 2 between the accumulated voltage V p and the specified voltage V r is used for determining whether the driving loop 3 is in the short circuit state.
  • the curved line b shows the detection voltage V out in the normal state
  • the curved line c shows the accumulated voltage V p .
  • the driving loop 3 with the specified driving electrode 31 is determined as being in the short circuit state according to the voltage difference ⁇ V 2 and the accumulated voltage V p .
  • the curved line of the EWOD device 100 being the normal state should first be detected as shown in FIG. 5 .
  • the curved line of the EWOD device 100 in the normal state serves as a standard line.
  • the change of the accumulated voltage V p is detected for determining the short circuit state or the open circuit state of the circuit in the EWOD device 100, and the position of the circuit in the EWOD device 100 is also detected.
  • the EWOD device 100 executes a self-detection of the detection chip 10 by the internal circuit of the EWOD device 100, and no external detection device is required.
  • the method for detecting the circuit in the EWOD device 100 is simple, and easily operated. The result of detection is more accurate. The method has higher efficiency, and a determination as to abnormal functioning is more accurate.
  • the switch module 30 is electrically connected to the specified driving electrode 31, thus the power input module 20 provides the power voltage V in to the specified driving electrode 31.
  • the specified driving electrode 31 couples with the detection electrode 32 to generate the detection voltage V out (coupled voltage), and the detection electrode 32 outputs the detection voltage V out to the detection module 40.
  • the detection module 40 accumulates the detection voltage V out to obtain the accumulated voltage V p .
  • the determination module 50 compares the accumulated voltage V p with the specified voltage V r to determine whether the circuit with the specified driving electrode 31 is in the short circuit state or the open circuit state, and the position of the circuit in the short circuit state or the open circuit state is also confirmed.
  • the determination process is the same as the above detection principle.
  • FIG. 10 shows a second embodiment of a circuit diagram of a dielectric wetting device 100 in a normal state.
  • the dielectric wetting device 100 includes the detection chip 10, a power input module 20, a switch module 30, a detection module 40, and a determination module 50.
  • the power input module 20 is electrically connected to the detection chip 10 through the switch module 30.
  • the power input module 20 is electrically connected to the driving electrodes 31 of the detection chip 10 through the switch module 30 and applies a power voltage V in to the driving electrodes 31.
  • the switch module 30 connects the driving electrodes 31 and the power input module 20.
  • the switch module 30 includes a plurality of switch units 4. Each switch unit 4 is electrically connected to one of the driving electrodes 31.
  • the detection electrode 32 receives a detection voltage V out (coupled voltage) and outputs the detection voltage V out .
  • the detection module 40 is electrically connected to the detection electrode 32.
  • the detection module 40 receives the detection voltage V out outputted by the detection electrode 32, and accumulates the detection voltage V out to obtain an accumulation voltage V p .
  • a sight deviation signal can be accumulated, and when the accumulated voltage V p reaches a specified voltage V r , the accumulated voltage V p is outputted.
  • an error or potential error is removed, and veracity of detection is improved.
  • the detection module 40 includes a voltage accumulation circuit 41.
  • the voltage accumulation circuit 41 includes an operational amplifier U and a first capacitor C 1 .
  • An output terminal of the detection electrode 32 is electrically connected to a positive terminal of the operational amplifier U and a terminal of the first capacitor C 1 .
  • Another terminal of the first capacitor C 1 is electrically connected to an output terminal of the operational amplifier U.
  • a positive terminal of the operational amplifier U is grounded.
  • the output terminal of the operational amplifier U serves as an output terminal of the detection module 40 for outputting the accumulated voltage V p of the detection voltage V out .
  • the voltage accumulation circuit 41 includes an integrator.
  • the voltage accumulation circuit 41 can include the voltage accumulation circuit 41, not being limited.
  • the detection module 40 also can include other circuits, such as a filter circuit.
  • the first dielectric layer 33 and the second dielectric layer 34 are hydrophobic insulation layers, such as polytetrafluoroethylene coating.
  • the first dielectric layer 33 and the second dielectric layer 34 present an insulating and hydrophobic function, the droplet D smoothly moves along the specified path, and fragmentation or breakage of the droplet is prevented while the droplet D being moved.
  • FIG. 11 is a circuit diagram of the EWOD device 100 in one embodiment.
  • Equivalent capacitors are formed in the driving loop 3 between the first dielectric layer 33, the second dielectric layer 34, and the channel 2 of the detection chip 10.
  • the first dielectric layer 33 forms a first dielectric capacitor C di-B in the driving loop 3.
  • the second dielectric layer 34 forms a second dielectric capacitor C di-T .
  • the channel 2 between the first dielectric layer 33 and the second dielectric layer 34 without silicone oil forms an equivalent air capacitor C air .
  • the capacitance of the equivalent air capacitor C air is changed according to a quantity of the silicone oil in the channel 2 between the first dielectric layer 33 and the second dielectric layer 34.
  • each driving loop 3 formed by each driving electrode 31 the first dielectric capacitor C di-B , the air capacitor C air , and the second dielectric capacitor C di-T are electrically connected in series.
  • a terminal of the first dielectric capacitor C di-B away from the air capacitor C air is electrically connected to the corresponding driving electrode 31, and a terminal of the second dielectric capacitor C di-T away from the air capacitor C air is electrically connected to the detection electrode 32.
  • a first resistor (R BA , R BB , R BC ) (equivalent resistor) and a second capacitor (C BA , C BB , C BC ) (equivalent capacitor) are formed based on the wire connecting the switch unit 4 and the driving electrodes 31.
  • the first resistor (R BA , R BB , R BC ) and the second capacitor (C BA , C BB , C BC ) are electrically connected in series.
  • a terminal of the first resistor (R BA , R BB , R BC ) is electrically connected to the switch unit 4, and another terminal of the first resistor (R BA , R BB , R BC ) is electrically connected to the corresponding second capacitor (C BA , C BB , C BC ) and the corresponding driving electrode 31.
  • Another terminal of the second capacitor (C BA , C BB , C BC ) is grounded.
  • a second resistor R T (equivalent resistor) is formed by the wire connected between the detection electrode 32 and the detection module 40.
  • the power voltage V in outputted by the power input module 20 is a continuous square pulsed voltage.
  • the detection voltage V out also is a continuous square pulsed voltage.
  • the voltage accumulation circuit 41 When the detection electrode 32 outputs the detection voltage V out to the voltage accumulation circuit 41, the voltage accumulation circuit 41 accumulates the detection voltage V out to obtain the accumulation voltage V p .
  • the detection module 40 outputs the accumulation voltage V p to the determination module 50.
  • the determination module 50 compares the accumulation voltage V p with the specified voltage V r .
  • An open circuit state and a short circuit state in the driving loop 3 can be determined by a difference between the accumulation voltage V p and the specified voltage V r . The position of the driving loop 3 in the open circuit state or the short circuit state is also confirmed.
  • the accumulated voltage V p of the driving loop 3 in a normal state firstly needs to be detected for serving as the specified voltage V r .
  • the accumulated voltage V p of the driving loop 3 is equal to the specified voltage V r .
  • the circuit detection principle of the EWOD device 100 will be described as below.
  • FIG. 11 shows the circuit diagram of the EWOD device 100
  • FIG. 12 shows waveforms of voltages of the EWOD device 100.
  • the power voltage V in of the power input module 20 (the continuous square pulsed voltage as shown in FIG. 12 ) is provided to a driving loop 3 with a specified driving electrode 31 through the switch module 30.
  • the detection voltage V out is outputted by the detection electrode 32.
  • the detection voltage V out is accumulated by the voltage accumulation circuit 41 (an integrator) of the detection module 40 to obtain the accumulated voltage V p (as shown in FIG. 12 ).
  • the detection module 40 outputs the accumulated voltage V p to the determination module 50 for comparing.
  • the electrode A and the detection electrode 32 form a driving loop 3.
  • the continuous square pulsed voltage of the power input module 20 is provided to the electrode A through the equivalent resistor R BA , the electrode A couples with the detection electrode 32, and the detection electrode 32 outputs the detection voltage V out (coupled voltage) to the voltage accumulation circuit 41 (integrator) through the equivalent resistor between the detection electrode 32 and the detection module 40.
  • the voltage accumulation circuit 41 accumulates the detection voltage V out to obtain the accumulated voltage V p , and outputs the accumulated voltage V p to the determination module 50.
  • the determination module 50 computes the difference between the accumulated voltage V p and the specified voltage V r to determine whether the EWOD device 100 is in a normal state.
  • a peak voltage of the detection voltage V out serves as the accumulated voltage V p of the EWOD device 100 in the normal state.
  • the waveform of the accumulated voltage V p is overlapped with the waveform of the specified voltage V r , and the accumulated voltage V p is equal to the specified voltage V r . Therefore, the EWOD device 100 in this situation is in the normal state.
  • FIG. 13 shows the circuit diagram of the EWOD device 100 in the open circuit state.
  • the power voltage V in of the power input module 20 (the continuous square pulsed voltage as shown in FIG. 14 ) is provided to a driving loop 3 with the specified driving electrode 31 through the switch module 30.
  • the driving loop 3 is in the open circuit state
  • the power voltage V in of the power module 20 is not provided to the specified electrode 31 through the switch module 30 and the detection electrode 32, and the detection electrode 32 does not output the detection voltage V out .
  • the voltage accumulation circuit 41 (integrator) of the detection module 40 does not receive the detection voltage V out , and does not accumulate the detection voltage V out to obtain the accumulated voltage V p . Therefore, the determination module 50 easily determines that the driving loop 3 of the specified driving electrode 31 is in the open circuit state.
  • the detection module 40 When the circuit with the electrode A is in the open circuit state, the detection module 40 does not receive the detection voltage V out to obtain the accumulated voltage V p .
  • the voltage difference ⁇ V 1 between the voltage detected by the detection module 40 and the specified voltage V r is used for determining whether the driving loop 3 is in the open circuit state.
  • the waveform of the detection voltage V out in the normal state with the peak voltage is equal to the specified voltage V r .
  • the waveform of the detection voltage V out is the straight line below the waveform of the detection voltage V out in the normal state.
  • the detection module 40 does not receive the detection voltage V out , thus there is no accumulated voltage V p .
  • the straight line in FIG. 14 represents the accumulated voltage V p .
  • FIG. 15 shows the circuit diagram of the EWOD device 100 in the short circuit state.
  • the power voltage V in of the power input module 20 (the continuous square pulsed voltage as shown in FIG. 16 ) is provided to a driving loop 3 with the specified driving electrode 31 through the switch module 30.
  • the detection electrode 32 outputs the detection voltage V out
  • the voltage accumulation circuit 41 (integrator) of the detection module 40 accumulates the detection voltage V out to obtain the accumulated voltage V p .
  • the detection module 40 outputs the accumulated voltage V p to the determination module 50 for comparing.
  • the power voltage V in of the power module 20 is not provided to the circuit through the switch module 30, different wires between the specified driving electrode 31 are electrically connected with each other, the resistance of the first resistors (R BA , R BB , R BC ) being driven is increased, and the accumulated voltage V p decreases.
  • the voltage difference ⁇ V 2 between the accumulated voltage V p and the specified voltage V r is used for determining whether the driving loop 3 is in the short circuit state.
  • the curved line b shows the detection voltage V out in the normal state
  • the curved line c shows the accumulated voltage V p .
  • the driving loop 3 When the driving loop 3 is in the short circuit state, a slope of the curved line c of the accumulated voltage V p is less than a slope of the curved line b of the detection voltage V out in the normal state.
  • the voltage difference ⁇ V 2 in the short circuit state is less than the voltage difference ⁇ V 1 in the open circuit state. Therefore, the driving loop 3 with the specified driving electrode 31 is determined as being short circuited according to the voltage difference ⁇ V 2 and the accumulated voltage V p .
  • the driving loop 3 with the specified driving electrode 31 is determined as being in the short circuit state according to the voltage difference ⁇ V 2 and the accumulated voltage V p .
  • the curved line of the EWOD device 100 being the normal state should be detected firstly as shown in FIG. 12 .
  • the curved line of the EWOD device 100 in the normal state serves as a standard line.
  • the change of the accumulated voltage V p is detected for determining the short circuit state or the open circuit state of the circuit in the EWOD device 100, and the position of the circuit in the EWOD device 100 is also detected.
  • the EWOD device 100 executes a self-detection of the detection chip 10 by the internal circuit of the EWOD device 100, and no external detection device is required.
  • the method for detecting the circuit in the EWOD device 100 is simple, and is easy for operation. The detection result is more accurate. The method has higher efficiency, and a determination as to abnormal functioning is more accurate.
  • a method for detecting a circuit in the EWOD device 100 includes at least the following steps, which also may be followed in a different order:
  • the switch module 30 is electrically connected to the specified driving electrode 31, thus the power input module 20 provides the power voltage V in to the specified driving electrode 31.
  • the detection module 40 accumulates the detection voltage V out to obtain the accumulated voltage V p .
  • the determination module 50 compares the accumulated voltage V p with the specified voltage V r to determine whether the circuit with the specified driving electrode 31 is in the short circuit state or the open circuit state, and the position of the circuit in the short circuit state or the open circuit state is also confirmed.
  • the determination process is the same as the above detection principle.
  • the EWOD device 100 can execute a self-detection for detecting the internal circuits. By comparing the accumulated voltage V p and the specified voltage V r , the state of the circuit in the EWOD device 100 is confirmed, such as the open circuit state and the short circuit state, and the position of the circuit in the EWOD device 100 is also confirmed.
  • the method for detecting the circuit in the EWOD device 100 is simple, and is easy for operation. The detection result is more accurate. The method has higher efficiency, and a determination as to abnormal functioning is more accurate

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  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Testing Of Short-Circuits, Discontinuities, Leakage, Or Incorrect Line Connections (AREA)
EP21199850.5A 2020-09-30 2021-09-29 Verfahren zur bereitstellung von selbstdetektion einer bedingung mit offener schaltung oder geschlossener schaltung in einer dielektrischen vorrichtung Withdrawn EP3978129A1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063085368P 2020-09-30 2020-09-30
US202063085385P 2020-09-30 2020-09-30
US202163137597P 2021-01-14 2021-01-14
CN202110744774.5A CN114336510A (zh) 2020-09-30 2021-07-01 介电润湿装置及其电路检测方法
CN202110746173.8A CN114336511A (zh) 2020-09-30 2021-07-01 介电润湿装置及其电路检测方法

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EP3978129A1 true EP3978129A1 (de) 2022-04-06

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EP21199850.5A Withdrawn EP3978129A1 (de) 2020-09-30 2021-09-29 Verfahren zur bereitstellung von selbstdetektion einer bedingung mit offener schaltung oder geschlossener schaltung in einer dielektrischen vorrichtung

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018039281A1 (en) * 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
US20200175932A1 (en) * 2018-12-03 2020-06-04 Sharp Life Science (Eu) Limited Am-ewod circuit configuration with sensing column detection circuit
US20200222899A1 (en) * 2019-01-15 2020-07-16 Board Of Regents, The University Of Texas System Electrowetting on dielectric (ewod) device to perform liquid-to-liquid extraction (lle) of biomolecules and systems and methods for using the ewod device

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018039281A1 (en) * 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
US20200175932A1 (en) * 2018-12-03 2020-06-04 Sharp Life Science (Eu) Limited Am-ewod circuit configuration with sensing column detection circuit
US20200222899A1 (en) * 2019-01-15 2020-07-16 Board Of Regents, The University Of Texas System Electrowetting on dielectric (ewod) device to perform liquid-to-liquid extraction (lle) of biomolecules and systems and methods for using the ewod device

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