EP3962444A1 - Composant d'alkyle d'ammonium quaternaire contenant une composition de soin buccal pour le traitement des caries - Google Patents

Composant d'alkyle d'ammonium quaternaire contenant une composition de soin buccal pour le traitement des caries

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Publication number
EP3962444A1
EP3962444A1 EP20721318.2A EP20721318A EP3962444A1 EP 3962444 A1 EP3962444 A1 EP 3962444A1 EP 20721318 A EP20721318 A EP 20721318A EP 3962444 A1 EP3962444 A1 EP 3962444A1
Authority
EP
European Patent Office
Prior art keywords
composition
quaternary ammonium
lactic acid
oral care
care composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20721318.2A
Other languages
German (de)
English (en)
Inventor
Ingo Haeberlein
Brenda Schmid
Melanie Hauke
Ajmal SAFI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
3M Innovative Properties Co
Original Assignee
3M Innovative Properties Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3M Innovative Properties Co filed Critical 3M Innovative Properties Co
Publication of EP3962444A1 publication Critical patent/EP3962444A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/41—Amines
    • A61K8/416—Quaternary ammonium compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/13—Amines
    • A61K31/14—Quaternary ammonium compounds, e.g. edrophonium, choline
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
    • A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
    • A61K8/492—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid having condensed rings, e.g. indol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses

Definitions

  • the invention relates to a composition for use in a method of treating caries or reducing the risk of getting caries lesions by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a living human or animal being.
  • composition comprises certain quaternary ammonium alkyl component(s) dissolved or dispersed in water, optionally in combination with a carrier material.
  • Dental plaque which may include bacteria such as Streptococcus mutans, comprises a biofilm that forms on surfaces in the oral cavity. Dental plaque is at least partly responsible for dental caries, gingivitis, and periodontal diseases.
  • Dental plaque Bacteria in dental plaque metabolize carbohydrates (for example, simple sugars) in the mouth and produce organic acids that can etch tooth enamel, dentin, and cement. Dental plaque can serve as a substrate for the deposition of tartar or calculus. Build-up of dental plaque and calculus can lead to gingivitis and, ultimately, to periodontal disease.
  • carbohydrates for example, simple sugars
  • a currently available method to remove dental plaque from teeth is mechanical removal with, for example, dental floss or a toothbrush.
  • a toothbrush can aid in removing dental plaque from exposed surfaces of a tooth, and dental floss can aid in removing dental plaque from, for example, interproximal and subgingival surfaces.
  • Proper and regular use of dental floss and a toothbrush can mechanically remove or reduce dental plaque, and can reduce the incidence of dental caries, gingivitis, and periodontal disease.
  • Certain antimicrobial formulations are available (in the form of mouthwashes, rinses, and toothpastes, for example) to aid in the control and treatment of dental plaque, dental caries, gingivitis, and periodontal disease.
  • dental caries results from an imbalance of the metabolic activity in the individual dental biofilm. This reflects the daily clinical experiences that only a small fraction of dental plaque might end up in tooth demineralization. Even more, certain studies with dental biofilm revealed that the bacterial composition of dental plaque does not necessarily indicate the prevailing metabolic (caries) activity of the individual dental plaque.
  • WO 2014/094900 A1 (GABA International) relates to an oral care composition comprising an amine fluoride and an acidic polymer, wherein the amine fluoride and acidic polymer have first and second reactive groups which are arranged to react together to form a cross-linked copolymer of the amine fluoride and acidic polymer.
  • the cross- linked polymer is said to provide significant protection against acidic attack of the tooth surface.
  • US 4,088,752 (Muhlemann et al) describes an oral care composition for plaque and inhibition containing a salt of an oxo-acid of phosphorous or mixed salt of an oxo-acid of phosphorous and hydrofluoric acid with an organic base. It is outlined that it is advantageous if basic groups are neutralized with hydrogen fluoride. The use of sodium fluoride and sodium trimetaphosphate are said to be effective and that the cariostatic effect was additive.
  • Geradu et al. (Eur J Oral Science (2007), 115, 148-152) refers to a study about plaque formation and lactic acid production after the use of amine fluoride / stannous fluoride mouth rinses. It is outlined that a daily use of AmF / SnF 2 rinse does not result in a reduction of plaque formation or in a reduction of sucrose metabolism in buccal and interproximal plaque.
  • typically highly concentrated fluoride gels are topically applied to a patient's teeth.
  • a biofilm matrix such as dental plaque may contribute to the isolation of bacteria from the protective effect of antimicrobial compounds and, thus, may interfere with the function of antimicrobial formulations such as mouthwashes, rinses, and toothpastes.
  • alternative methods and compositions to control or prevent the risk of caries are desirable.
  • compositions which is able to influence metabolic balances residing in oral biofilms which are left in a patient’s mouth.
  • Such a composition should be easy in administering and simple in use.
  • the composition should be easy to prepare and reasonable in price of manufacturing.
  • composition should not have undesired side effects like bad taste or being astringent.
  • the present invention features a composition, in particular an oral care composition, for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a human being or animal, the composition comprising quaternary ammonium alkyl component(s) or mixtures thereof as described in the present text and claims dissolved or dispersed in water and being present in an amount of at least 0.005 wt.% with respect to the composition.
  • the invention is also related to a kit of parts as described in the present text and claims for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a human being or animal, the kit of parts containing Part A comprising quaternary ammonium alkyl component(s) or mixtures thereof as described in the present text and claims, Part B comprising water, and optionally Part C comprising an application device.
  • Described is also a method of using the composition or kit of parts as described in the present text and claims comprises the step of brining the composition in contact with oral biofilm and/or hard dental tissue in the mouth of a human being or animal.
  • the invention is also directed to the use of certain quaternary ammonium alkyl component(s) or mixtures thereof as described in the present text and claims for producing a composition or a kit of parts as described in the present text and claims for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a human being or animal.
  • composition is understood to be a mixture of two or more components.
  • a “dental or oral care composition” is a composition which is to be used in the dental field (including restorative and prosthodontic work) including the orthodontic area.
  • the composition should be not detrimental to the patient's health and thus free of hazardous and toxic components being able to migrate out of the composition.
  • Commercially available products have to fulfil certain requirements such as those given in DIN EN ISO 1942:2011-03.
  • a "dental surface” or“dental hard tissue” refers to tooth structures (e. g., enamel, dentin, and cementum) and bone.
  • A“tooth structure” is any tooth structure, prepared or ready for preparation by the dentist. It can be a single tooth or two or more teeth. A tooth structure is also referred to as hard dental tissue in contrast to soft dental tissue (e.g. gingival).
  • “Caries” is understood as tooth decay, also known as dental caries which is a demineralization and/or breakdown of teeth due to acids made by bacteria.
  • Distal plaque is understood as is a biofilm or mass of bacteria that grows on surfaces within the mouth.
  • biofilm refers to a matrix containing bacteria.
  • a biofilm in an oral cavity can include bacteria, epithelial cells, leukocytes, macrophages and further oral exudate.
  • A“paste” is a substance that behaves as a solid until a sufficiently large load or stress is applied, at which point it flows like a fluid.
  • Pastes typically consist of a suspension of granular material in a background fluid. The individual grains are jammed together like sand on a beach, forming a disordered, glassy or amorphous structure, and giving pastes their solid-like character. Pastes can be classified by their viscosity or their consistency comparable to dental impression material.
  • A“film forming component” is a substance which will cause a composition containing it to change from a liquid stage to a solid stage in such a manner as to form a film or coating on a surface.
  • a definition of the term“film former” can also be found in DIN 55945 (1999-07-00).
  • the term “film former” is often used in varnishes.
  • the substance hydroxyethyl methacrylate (HEMA) cannot be regarded as a film former according to this definition.
  • A“film” is a thin sheet or strip of a preferably flexible material. A coating of a surface with a material typically results in a film.
  • A“toothpaste” (dentifrice) is a cleaning agent for the daily individual care. It is typically used as a prophylactic measure against caries, gingivitis or periodontitis.
  • a“prophylaxis paste” is a product which is used by a profession such as a dentist or a dental hygienist to remove adherent deposits such as stain, plaque or tartar which may stick to the surface of a natural tooth, artificial tooth crown or bridge or filling material.
  • a prophylaxis paste is therefore typically used on slowly rotating paste carrier (sometimes also referred to as prophy cups). Most of the commercially available prophylaxis pastes have a different viscosity compared to toothpastes.
  • A“gel” is typically a colloidal system in which a porous matrix of interconnected particles spans the volume of a liquid medium.
  • gels are apparently solid, jelly- like materials. Both by weight and volume, gels are mostly liquid in composition and thus exhibit densities similar to liquids, however, have the structural coherence of a solid.
  • An example of a common gel is edible gelatine. Many gels display thixotropy, that is, they become fluid when agitated, but re-solidify when resting.
  • A“solvent” means a liquid which is able to at least partially disperse or dissolve a component at ambient conditions (e.g. 23°C). A solvent typically has a viscosity below 5 or below 1 or below 0.1 Pa*s at 23°C.
  • A“particle” means a substance being a solid having a shape which can be geometrically determined. Particles can typically be analysed with respect to e.g. grain size.
  • the mean particle size of a powder can be obtained from the cumulative curve of the grain size distribution and is defined as the arithmetic average of the measured grain sizes of a certain powder mixture. Respective measurements can be done using commercially available granulometers (e.g. CILAS Laser Diffraction Particle Size Analysis Instrument).
  • Ambient conditions mean the conditions which the inventive solution is usually subjected to during storage and handling.
  • Ambient conditions may, for example, be a pressure of 900 to 1100 mbar, a temperature of 10 to 40 °C and a relative humidity of 10 to 100 %. In the laboratory ambient conditions are adjusted to 20 to 25 °C and 1,000 to 1,025 mbar.
  • a composition is“essentially or substantially free of’ a certain component, if the composition does not contain said component as an essential feature. Thus, said component is not wilfully added to the composition either as such or in combination with other components or ingredient of other components.
  • a composition being essentially free of a certain component usually does not contain that component at all. However, sometimes the presence of a small amount of the said component is not avoidable e.g. due to impurities contained in the raw materials used.
  • “And/or” means one or both.
  • the expression component A and/or component B refers to a component A alone, component B alone, or to both component A and component B.
  • Adding an“(s)” to a term means that the term should include the singular and plural form.
  • the term“additive(s)” means one additive and more additives (e.g. 2, 3, 4, etc.).
  • the composition described in the present text provides an effective means for reducing the risk of caries lesions.
  • an in-vitro human saliva derived microcosm biofilm was used to apply the quaternary ammonium alky component described in the present text twice a day for at least 1 min each. In comparison to a control it was observed that an increasing concentration of the quaternary ammonium alkyl component lower lactic acid release of an oral microcosm biofilm substantially in presence of sucrose.
  • the quaternary ammonium alkyl component(s) can be used either as a biofilm metabolism control agent or as an agent for modifying the structure of the biofilm or as an agent functioning as a kind of cell membrane modifying agent. Independent of the mechanism, the quaternary ammonium alkyl component is effective for treating caries or reducing the risk of getting caries lesions.
  • the cell membrane modifying agent primarily refers to the cell membrane of the cells of the bacteria forming the biofilm and of the extra-cellular membranes constituting the biofilm.
  • the oral care composition described in the present text is designed to move from a plaque formation inhibition composition or dental plaque removal composition or biofilm reducing composition to a composition allowing the control of the metabolic balance on dental plaque.
  • the invention shows that certain quaternary ammonium alkyl component(s) are effective to accomplish caries activity control in a dental biofilm, i.e. being able to influence the metabolic balance of the dental biofilm.
  • the quaternary ammonium alky component(s) proposed in the present text are easily available at reasonable costs and essentially non-toxic to the patient.
  • the quaternary ammonium component described in the present text does not comprise fluoride ions, the risk that this component has side-effects due to undesired interaction of fluoride ions with the human body is reduced or sometimes even eliminated.
  • the oral care composition described in the present text for use in a method or therapy of treating caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm of a human or animal being comprises certain quaternary ammonium alkyl component(s) or mixtures thereof.
  • quaternary ammonium alkyl component(s) can be characterized by the following formula:
  • R being selected from C 10-20 alkyl and X being selected from chloride, (hydrogen)carbonate, (hydrogen)sulphate, (hydrogen/di-hydrogen)phosphate, wherein chloride is typically preferred.
  • the formula refers to the quaternary ammonium (hydrogen)chloride, (hydrogen)carbonate, (hydrogen)sulphate or (hydrogen/di-hydrogen)phosphate salts of N'- Octadecyltrimethylenediamino-N,N,N'-tris(2-ethanol).
  • the carbon chain R is typically selected from decyl, dodecyl (lauryl), terradecyl (myristyl), hexadecyl (cetyl, palmityl), octadecyl (stearyl), eicosanyl, 9-cis-octadecen-1-yl (oleyl), 9-trans- octadecen-1-yl (elaidyl), cis,cis-9,1 2-octadecadien-1-yl (linolyl), cis,cis,cis-9,12,15- octadecatrien-1-yl (linolenyl) or 9-cis-eicosaen-1-yl (gadolyl).
  • the quaternary ammonium alkyl component comprises at least one of N,N,N'- tris(2-hydroxyethyl)-N'-octadecylpropane-1, 3-diamine dihydrochloride, N,N-bis(2- hydroxyethyl)oleylamine hydrochloride, and N,N-bis(2-hydroxyethyl)octadecylamine hydrochloride.
  • a preferred ammonium component which can be used is N'- Octadecyltrimethylendiamino-,N,N' - tris(2-ethanol)-dihydrochloride having the following structure:
  • the reduction of the lactic acid release can be determined as described in the example section.
  • the quaternary ammonium alky component(s) according to the present text are present in the composition in an amount of at least 0.005 wt.% with respect to the whole composition.
  • the oral care composition also comprises water.
  • Water is used for dissolving the quaternary ammonium alky component(s) contained in the composition, at least partially.
  • Water is typically present in an amount sufficient to fully dissolve the quaternary ammonium alky component(s) and other optional components being present in the composition.
  • composition may comprise water in the following amounts:
  • Range 5 to 98 or 10 to 95 or 20 to 90 wt.%; wt.% with respect to the whole composition.
  • the ratio of quaternary ammonium alkyl component(s) to water is typically at least 0.005 / 100, or at least 0.05 / 100, or at least 0.1 / 100 with respect to weight.
  • the ratio of the quaternary ammonium component(s) to water can be in a range of 0.005 / 100 to 5 / 100, or in a range 0.05 / 100 to 2 / 100, or in a range of 0.1 / 100 to 1 / 100 with respect to weight.
  • the oral care composition may also comprise one or more carrier components.
  • the nature and structure of the carrier component is not particularly limited, unless the desired result cannot be achieved.
  • Carrier components may help to adjust the rheological properties of the composition, to support the application and/or to trigger the release of active agent(s) contained in the composition.
  • the carrier component is typically present in the following amount(s):
  • Suitable carrier components are gel-forming components or paste-forming components.
  • gel-forming or paste-forming components include Irish moss, carboxymethyl cellulose, gum tragacanth, gum arabic, gum Karaya, sodium alginate, hydroxyethyl cellulose, methyl and ethyl cellulose, carrageenan, xanthan gum, polyvinyl pyrrolidone, and mixtures thereof.
  • the carrier component is a film-forming agent.
  • Film-forming agents are suitable to improve adherence of the quaternary ammonium alkyl component(s) contained in the composition described in the present text to hard dental tissue, such as a tooth surface.
  • film-forming agent(s) are present, the components contained in the composition described in the present text are typically dissolved or dispersed in the film forming component(s) and form together with the film forming component(s) a film or coating on the surface of hard dental tissue.
  • the molecular weight (Mw) of the film-forming agent can vary over a wide range (e.g. 2,000 to 1,200,000 g/mol). Typical ranges include 10,000 to 500,000 or 20,000 to 300,000 g/mol.
  • the molecular weight can be determined by GPC technology, using e.g. a polystyrene standard.
  • the film forming component might not be able to form a sufficiently durable film or coating. Thus, the effect of a delayed release of the quaternary ammonium alkyl component might not be obtained.
  • components like 2-hydroxy ethyl acrylate (HEMA), which are sometimes classified as film-forming agents, are not suitable as film forming component in the sense of the present text.
  • the film caused or produced by the film-forming agent typically has a thickness in a range of 0.5 mm to 100 mm or 10 mm to 50 mm.
  • Film formers or film-forming agents can be classified as natural film former, semi synthetic film formers, cellulose derivatives, poly(meth)acrylates and vinyl polymers.
  • natural film-forming agents include shellac, mastix, sandarac, tolubalsam, dammar resin, benzoe resin, keratin, maizin, gum Arabic and gelatines.
  • semi-synthetic film-forming agents include gelatines treated with formaldehyde and salol (acetaldehyde phenol condensate).
  • Typical cellulose derivatives include cellulose acetate phthalate, hydroxyethylcellulose, hydroxypropylcellulose, methylcellulose, ethylcellulose and hydroxypropylmethyl cellulose phthalate.
  • poly(meth)acrylates examples include copolymers of (meth)acrylic esters and amino functional (meth)acrylates, copolymers of (meth)acrylic acid and methyl methacrylate, polyacrylamide, polyacrylic acid and salts thereof, in particular partial salts thereof, including sodium salts.
  • vinyl polymers include polyvinyl pyrrolidon, polyvinyl acetate phthalate (e.g. hydroxypropyl- and hydroxypropyl-methylcellulose), homo- and copolymers of polyvinylacetate, homo- and copolymers of polyvinylpropionate, styrene acrylics, ethylene vinyl acetate, poly(hydroxyethyl methacrylate, poly(vinylethylene glycol acrylate, polyvinyl alcohol(s).
  • polyvinyl pyrrolidon polyvinyl acetate phthalate (e.g. hydroxypropyl- and hydroxypropyl-methylcellulose), homo- and copolymers of polyvinylacetate, homo- and copolymers of polyvinylpropionate, styrene acrylics, ethylene vinyl acetate, poly(hydroxyethyl methacrylate, poly(vinylethylene glycol acrylate, polyvinyl alcohol(s).
  • film-forming agent(s) include (e.g. fully or partially hydrolyzed) polyvinylalcohol, polymethylvinylether, polyvinylpyrrolidone, (e.g. aqueous) acrylic resin dispersions (e.g. EudragitTM, commercially available from Rohm), gelatine, polysaccharides (e.g. agarose), polyacrylamide, copolymers of vinylpyrrolidinone and acrylamide, hydrophilic cellulose derivatives (e.g.
  • hydroxyethylcellulose hydroxypropylcellulose, methylcellulose
  • homo- and copolymers of polyvinylacetate homo- and copolymers of polyvinylpropionate, styrene acrylics, ethylene vinyl acetate, polyurethanes, hydroxylated acrylates such as poly(hydroxyethyl methacrylate), poly(vinylethylene glycol acrylate), and combinations and mixtures thereof.
  • film-forming agents which are sometimes preferred include polyvinyl alcohol, gelatine, polyacrylic acid, partially neutralized polyacrylic acid and mixtures thereof.
  • a film-forming agent is present, it is typically present in the following amounts:
  • composition described in the present text may also comprise abrasive particles.
  • abrasive particles examples include perlite, bentonite, silica, alumina, aluminium hydroxide, ilmenite (FeTiO 3 ), zircon oxide, zircon silicate, calcium carbonate, sodium bicarbonate, titanium dioxide, precipitated lime, chalk, flour of pumice, zeolites, talcum, kaolin, kieselguhr, aluminium oxide, silicates and mixtures thereof.
  • the oral care composition described in the present text for use in a method or therapy of modifying the structure of an oral biofilm containing lactic acid producing bacteria of a living human or animal being may also comprise one or more amino acids.
  • the amino component(s) are used for modifying the structure of the oral biofilm and thus have an influence on the overall environment where the lactic acid producing bacteria are located.
  • the amino acid(s) have a direct impact on the metabolism of the lactic acid releasing bacteria.
  • amino acids were found to be useful for achieving the desired results: glycine, leucine, isoleucine, lysine, methionine, phenylalanine, serine, threonine, valine, tryptophan and mixtures thereof, with the following amino acids being sometimes preferred: glycine, phenylalanine, serine, isoleucine, leucine, methionine, with glycine and phenylalanine being sometimes being even more preferred.
  • the amino acids may be natural or synthetic.
  • amino acids might be in D- or L-configuration, wherein the L-configuration is preferred.
  • the composition described in the present text does typically not contain these amino acids in an effective amount, e.g. more than 0.5 or more than 0.3 or more than 0.1 wt.%.
  • amino acids selected from glycine, leucine, isoleucine, lysine, methionine, phenylalanine, serine, threonine, valine, tryptophan and mixtures thereof are present in an amount sufficient for achieving the desired result.
  • the amino acids contained in the oral care composition are used in a therapeutically effective amount being sufficient to influence the lactic acid release metabolism of lactic acid releasing bacteria in an oral biofilm.
  • composition and the amino acids contained therein are used for a time period being sufficient to influence the lactic acid release metabolism of lactic acid releasing bacteria in an oral biofilm.
  • composition and the amino acids contained therein are typically present in an amount and applied for a time period effective to reduce the lactic acid release of lactic acid producing bacteria by more than 40% or more than 45% compared to the situation before the oral care composition described in the present text was used.
  • amino acid(s) are typically present in the following amounts: Lower limit: at least 0.1 or at least 1 or at least 2 wt.%;
  • Range 0.1 to 15 or 1 to 12 or 2 to 10 wt.%;
  • glycine in an amount of 0.1 to 10 wt.%; or
  • leucine in an amount of 0.1 to 5 wt.%; or
  • isoleucine in an amount of 0.1 to 5 wt.%; or
  • lysine in an amount of 0.1 to 10 wt.%; or
  • methionine in an amount of 0.1 to 10 wt.%; or
  • phenylalanine in an amount of 0.1 to 5 wt.%; or
  • threonine in an amount of 0.1 tolO wt.%; or
  • valine in an amount of 0.1 to 8 wt.%; or
  • tryptophan in an amount of 0.1 to 2 wt.%
  • glycine in an amount of 1 to 10 wt.%; or
  • lysine in an amount of 5 to 10 wt.%;
  • phenylalanine in an amount of 1 to 5 wt.%;
  • threonine in an amount of 6 to 10 wt.%;
  • tryptophan in an amount of 0.5 to 2 wt.%
  • the ratio of amino acid(s) to water is typically at least 0.1 / 100, or at least 1.5 / 100, or at least 2 / 100 with respect to weight.
  • the ratio of amino acid(s) to water can be in a range of 0.1 / 100 to 15 / 100, or in a range / 1.5 to 100 to 12 / 100, or in a range of 2 to 100 to 10 to 100 with respect to weight.
  • the ratio of the quaternary ammonium alkyl component(s) to amino acid(s) is typically at least 1 / 0.5 or at least 1 / 1 or at least 1 / 2.
  • the ratio of the quaternary ammonium alkyl component(s) to amino acid(s) can be in a range of 1 / 0.5 to 0.1 / 5, or in a range 1 / 1 to 1 / 5, or in a range of 1 / 2 to 1 / 5 with respect to weight.
  • the oral care composition described in the present text may also comprise additive(s).
  • One or more additives can be present, if desired.
  • additive(s) are typically present in the following amount(s):
  • Additive(s) which might be present include stabilizer(s), fluoride releasing agent(s), colourant(s), phosphate releasing agent(s), calcium releasing agent(s), anti microbial agent(s), buffer(s), surfactant(s), humectant(s), preservative agent(s), flavour additive(s) and mixtures thereof.
  • the composition might contain one or more stabilize ⁇ s) as an additive.
  • the storage stability of the dental composition might be improved. That is, the individual components of the composition do not separate over time.
  • a composition is defined as storage-stable, if the components do not separate from each other within 6 months or 12 months or 24 months or 36 months at ambient conditions. If a stabilizer is present, it is typically, present in a low amount.
  • Amounts, found to be useful, include 0.01 to 3 wt.% or 0.1 to 1 wt.% with respect to the weight of the whole composition.
  • stabilizer(s) examples include copolymers of 2,5-furandione with 1,9-decadiene and methoxyethene (e.g. StabilizeTM, International Specialty Products (ISP) Comp.) and carboxy vinyl polymers (e.g. CarbopolTM, Lubrizol Advanced Materials Comp.).
  • Stabilizers typically have a mean particle size below 500 mm or below 250 mm or below 100 mm.
  • the composition comprises one or more colourants. There is no need for a colourant to be present at all.
  • Typical ranges include 0.01 wt.% to 5 wt.% or 0.1 wt.% to 3 wt.% with respect to the whole composition.
  • the presence of a colourant may allow an easy detection in a patient's mouth
  • Colouring of the dental composition can be achieved by incorporating colorants or pigments (organic and inorganic) into the composition.
  • colourants examples include red iron oxide 3395, BayferroxTM 920 Z Yellow,
  • NeazoponTM Blue 807 copper phthalocyanine-based dye
  • Helio Fast Yellow ER and mixtures thereof.
  • the composition comprises one or more phosphate releasing agent(s) as an additive.
  • the composition comprises anti-microbial agent(s).
  • Typical ranges include 0.01 wt.% to 2 wt.% or 0.1 wt.% to 1 wt.% with respect to the whole composition.
  • an anti-microbial agent might help reducing health risks for professionals in the dental offices and laboratories as well as for patients.
  • Useful anti-microbial agents include chlorhexidine or derivatives thereof and aldehydes (glutaraldyde, phthalaldehyde) and chlorhexidine or its derivatives and salts of phenolics or acids. It can also be preferred to use acid adducts of chlorhexidine or its derivatives like e.g., acetates, gluconates, chlorides, nitrates, sulphates or carbonates.
  • Chlorhexidine and its derivatives are commercially available in water-based solutions (e.g. a 20 % aqueous solution of CHX di gluconate, CAS 18472-51-0) or as a pure compound or as a salt.
  • water-based solutions e.g. a 20 % aqueous solution of CHX di gluconate, CAS 18472-51-0
  • pure Chlorohexidine compound CAS 55-56-1
  • CHX salts like CHX diacetate monohydrate (CAS 56-95-1) or CHX dihydrochloride (CAS 3697-42-5) are preferred.
  • CHX also seems to be especially suited as an additive due in part to its well-known and proven anti-microbial action against Gram-positive and Gram-negative microorganisms including the oral Streptococci and Lactobacilli.
  • CHX is bacteriostatic for Mycobaterium.
  • CHX is also active against yeasts including Candida albicans and viruses including HIV, HBV, HCV, Influenza- and Herpes virus.
  • a further advantage of CHX is its low toxicity.
  • Preferred anti-microbial agents include hexitidin, cetypyridiniumcloride (CPC), chlorhexidin (CHX), triclosan, stannous chloride, benzalkonium chloride, non-ionic or ionic surfactants (e.g. quaternary ammonium compounds), alcohols [monomeric, polymeric, mono-alcohols, poly-alcohols (e. g. xylitol, sorbitol), aromatic (e. g. phenol)], antimicrobial peptides (e. g. histatins), bactericins (e. g. nisin), antibiotics (e. g. tetracycline), aldehydes (e.
  • the composition can comprise one or more buffer(s) as an additive. There is no need for a buffer to be present at all.
  • Typical ranges include 0.1 wt.% to 5 wt.% or 1 wt.% to 3 wt.% with respect to the whole composition.
  • buffers examples include acetic acid/acetate, tris(hydroxy- methyl)aminomethane (TRIS), N-(2-acetamido)-2-aminoethane sulfonic acid (ACES), N- (2-acetamido)imminodiacetate (ADA), N,N-bis(2-hydroxyethyl)-2-aminoethane sulfonic acid (BES), 2, 2-bis-(hydroxyethyl)-iminotris(hydroxylmethyl)m ethane (BIS-TRIS), 2- (cyclohexylamino)ethane sulfonic acid (CHES), 2-[4-(2-hy droxy ethyl- 1- piperazine)]ethane sulfonic acid (HEPES), 3-[4-(2-hydroxyethyl-1-piperazinyl)]propane sulfonic acid (HEPPS), 2-morpholinoethane sulfonic acid
  • the dental composition comprises one or more surfactant(s) as an additive. There is no need for a surfactant to be present at all.
  • surfactant(s) which can be used include water-soluble salts of alkyl sulphates and alkyl ether sulphates having 8 to 18 carbon atoms in the alkyl moiety, water-soluble salts of sulfonated monoglycerides of fatty acids having 8 to 18 carbon atoms in the alkyl radical and mixtures thereof.
  • compositions might contain one or more humectant(s) as an additive. There is no need for a humectant to be present at all.
  • Typical ranges include 0.01 wt.% to 5 wt.% or 0.1 wt.% to 3 wt.% with respect to the whole composition.
  • humectant(s) examples include glycerine, sorbitol, mannitol, xylitol, propylene glycol, polyethylene glycol and mixtures thereof.
  • preservative agent(s) examples include sodium benzoate, citric acid and its salts, and combinations thereof.
  • flavour additive(s) examples include peppermint, spearmint, and combinations thereof.
  • the composition comprises, consists essentially of or consists of:
  • Component A (quaternary ammonium alkyl component) in an amount of 0.005 to 5 wt.%;
  • Component B (water) in an amount of 5 to 98 wt.%;
  • Component C (carrier component) in an amount of 0 to 90 wt.%
  • Component D (amino acid) in an amount of 0 to 15 wt.%
  • Component E additive in an amount of 0 to 10 wt.%
  • the composition comprises, consists essentially of or consists of:
  • Component A (quaternary ammonium alkyl component) in an amount of 0.05 to 5 wt.%;
  • Component B water in an amount of 5 to 98 wt.%
  • Component C carrier component in an amount of 1 to 90 wt.%
  • Component D (amino acid) in an amount of 0 to 15 wt.%
  • Component E additive in an amount of 0 to 10 wt.%
  • the composition comprises, consists essentially of or consists of:
  • Component A (quaternary ammonium alkyl component) in an amount of 0.1 to 2 wt.%;
  • Component B (water) in an amount of 5 to 98 wt.%;
  • Component C (carrier component) in an amount of 1 to 90 wt.%;
  • Component D (amino acid) in an amount of 0.1 to 15 wt.%;
  • Component E additive in an amount of 0.1 to 10 wt.%
  • the composition typically has a pH value in the range of 6 to 8. Thus, the composition is essentially neutral.
  • the composition can also be characterized by its viscosity. Depending on its chemical formulation, the viscosity may vary over a huge range.
  • the viscosity is typically in a range of 1 to 10 mPa*s or 1 to 1,000 mPa*s at 23°C.
  • the viscosity is typically in a range of 2,000 to 200,000 mPa*s at 23°C.
  • the viscosity of liquids can be measured using a Physica MCR 301 Rheometer (Anton Paar, Graz, Austria) with a cone/plate geometry CP25-1 under controlled shear rate at 23 °C (e.g. 100 s -1 ).
  • the diameter is 25 mm, the cone angle 1°, and the separation between the cone tip and the plate 49 mm.
  • the viscosity of pastes can be determined using a Physica MCR 301 Rheometer (Anton Paar, Graz, Austria) with a plate/plate geometry (PP15) at a constant shear rate of 1 s -1 in rotation at 28 °C.
  • the diameter of the plates is 10 mm and the gap between the plates is set to 2.0 mm.
  • the composition described in the present text can typically be produced as follows:
  • the quaternary ammonium alkyl component(s) is provided and mixed with water, the optional carrier component(s), amino acid(s) and the optional additive(s).
  • the mixing is done by dissolving or dispersing the amino acid(s) in the carrier component(s) and additive(s), if desired, with the aid of a mixing device.
  • the mixing can be accomplished shortly before the use of the oral care composition or the oral care composition can already be provided in a mixed and storage stable form.
  • the present invention is also directed to a kit of parts for use in a method or therapy of reducing the caries activity by reducing the lactic acid release in an oral biofilm.
  • the kit of parts typically comprises
  • Part A comprising the quaternary ammonium alkyl component(s) described in the present text
  • Part B comprising water for dissolving or dispersing the quaternary ammonium component(s), optionally in combination with a carrier component,
  • Part C comprising an application device.
  • the other components such as amino acid(s), carrier component(s) or additive(s) as described in the present text can be present in Part A or Part B or Part A and Part B, as desired.
  • Providing the components of the composition in separated parts can be beneficial to improve the storage stability.
  • the practitioner Before use, the practitioner will prepare the oral care composition by combining the respective components of the individual parts.
  • the application of the oral care composition can be done by various means and/or using various devices.
  • Clear tray aligners can straighten a dental patient’s teeth without the need for using wires and brackets of traditional braces.
  • the aligners typically consist of a sequence of clear, removable trays that fit over the teeth to straighten them.
  • composition or kit of parts described in the present text in combination with a dental tray, mouth guard or clear tray aligner can be advantageous, as these kinds of devices are typically worn for a longer period of time (e.g. 10 min to 12 hrs) and thus are well suited for applying the oral care composition described in the present text for a longer period of time, if desired.
  • compositions described in the present text for use of the composition described in the present text in combination with a dental tray, mouth guard or clear tray aligner, providing the composition in the form of a paste or gel was found to be advantageous.
  • the oral care composition described in the present text is typically packaged in a suitable packaging device.
  • the size and shape of the packaging device typically depends on the form how the composition is provided.
  • Suitable packaging devices include sealable bottles, tubes, vessels or foil bags (including glass or plastic bottles, e.g. equipped with a screw cap), blisters, syringes, etc.
  • the packaging device might be designed for single-use or repeated use.
  • the quaternary ammonium alkyl component is used for producing an oral care composition or a kit of parts for a particular use as described in the present text.
  • the quaternary ammonium alkyl component is used as an agent for treating caries or reducing the risk of getting carious lesions by modifying the structure of an oral biofilm containing lactic acid producing bacteria.
  • composition described in the present text may be provided in different forms or shapes.
  • the composition is provided as liquid, e.g. in the form of an oral rinse or a mouth wash.
  • the composition is provided as gel.
  • the composition is provided as paste, e.g. in the form of a toothpaste.
  • the composition is provided as gum, e.g. in the form of a chewing gum.
  • the composition is provided in a form which results in a coating after application.
  • the composition has to be brought in contact with the oral biofilm or hard dental tissue.
  • the oral biofilm is typically located on tooth surfaces, in particular hard dental tissue.
  • the bringing into contact can be achieved by different means, including rinsing, spraying, brushing, swabbing, coating or combinations thereof.
  • the bringing into contact is typically done for a time period being sufficient for causing the desired effect.
  • the bringing into contact is typically done for a duration of at least 1 min or of at least 2 min or at least 3 min or at least 4 min.
  • the step of bringing into contact can be repeated several times.
  • the oral care composition is applied in periodic application scheme.
  • Possible daily repeating schemes for a periodic application scheme are:
  • the oral care composition is applied in a continuous application scheme.
  • composition described in the present text is applied to form a coating or film which can persist on a tooth surface for at least 2 or 5 or 10 days up to 12 months or even longer.
  • Such a composition typically contains film-forming agent(s).
  • Suitable film-forming agents are described above.
  • All components used in the dental composition of the invention should be sufficiently biocompatible, that is, the composition should not produce a toxic, injurious, or immunological response in living tissue.
  • composition described in the present text does typically not comprise the following components alone or in combination:
  • heavy metal components comprising Zn, Sn or Cu in an amount of more than 0.1 wt.%;
  • composition described in the present text are essentially free of abrasive particles, in particular free of the abrasive particles described in the text above.
  • Essentially free means less than 1 or less than 0.5 or less than 0.1 wt.% or do not contain abrasive particles at all. Common to most of these substances is typically a comparable high hardness, e.g. above about Mohs 4 or above about 5.
  • compositions described in the present text are essentially free of oxidizing component(s) (e.g. less than 0.5 or less than 0.3 or less than 0.1 wt.%) or do not contain oxidizing components at all.
  • Oxidizing component(s) which are typically not present are peroxide, hypochlorite, perborate, persulfate, peroxyphosphate, peroxycarbonate.
  • oxidizing components have a negative effect in that they may react with the amino acids being present in the composition having the result that the reaction products are no longer suitable for acting as a metabolism modifying agent.
  • compositions described in the present text are essentially free of heavy metal component(s), in particular those comprising Zn, Sn or Cu (e.g. less than 0.1 or less than 0.05 or less than 0.01 wt.%) or do not contain heavy metal component(s) at all.
  • heavy metal components may have a negative effect, too, in that they may react with the amino acids being present in the composition (e.g. by forming insoluble complexes) having the result that the reaction products are no longer suitable for acting as a metabolism modifying agent.
  • An oral care composition for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a living human or animal, the composition comprising
  • quaternary ammonium alkyl component(s) in an amount of at least 0.05 wt.%, water,
  • composition having a pH in the range of 6 to 8.
  • the quaternary ammonium alkyl component being characterized by the following formula: R-N(CH 2 CH 2 OH)-CH 2 CH 2 CH 2 -N(CH 2 CH 2 OH) 2 * 2HX, with R being selected from C 10-20 alkyl and X being selected from chloride, carbonate, sulphate, phosphate, wherein chloride is typically preferred.
  • R being selected from C 10-20 alkyl
  • X being selected from chloride, carbonate, sulphate, phosphate, wherein chloride is typically preferred.
  • An oral care composition for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a living human or animal being, the composition comprising quaternary ammonium alkyl component(s) in an amount of at least 0.05 wt.%, water,
  • the ratio of quaternary ammonium alkyl component to water being in a range of 0.05: 100 to 5: 100 with respect to weight
  • composition having a pH in the range of 6-8,
  • the quaternary ammonium alkyl component being N'-Octadecyltrimethylendiamino-N,N,N' -tris(2-ethanol)-dihydrochloride.
  • An oral care composition for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a living human or animal being, the composition comprising quaternary ammonium alkyl component(s) in an amount of at least 0.1 wt.%, water,
  • the ratio of quaternary ammonium alkyl component to water being in a range of 0.1 : 100 to 5: 100 with respect to weight
  • composition having a pH in the range of 6-8 and
  • the quaternary ammonium alkyl component being characterized by the following formula: R-N(CH 2 CH 2 OH)-CH 2 CH 2 CH 2 -N(CH 2 CH 2 OH) 2 * 2HX, with R being selected from C 10-20 alkyl and X being selected from chloride, carbonate, sulphate, phosphate, wherein chloride is typically preferred.
  • R being selected from C 10-20 alkyl
  • X being selected from chloride, carbonate, sulphate, phosphate, wherein chloride is typically preferred.
  • An oral care composition for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a living human or animal being, the composition comprising quaternary ammonium alkyl component(s) in an amount of at least 0.05 wt.%, water,
  • amino acid(s) selected from glycine, leucine, isoleucine, lysine, methionine, phenylalanine, serine, threonine, valine, tryptophan and mixtures thereof,
  • the method of treating caries by reducing the lactic acid release comprising the step of applying the composition being applied to the oral biofilm and/or hard dental tissue for at least 1 min,
  • the quaternary ammonium alkyl component being characterized by the following formula: R-N(CH 2 CH 2 OH)-CH 2 CH 2 CH 2 -N(CH 2 CH 2 OH) 2 * 2HX, with R being selected from C 10- 20 alkyl and X being selected from chloride, carbonate, sulphate, phosphate, wherein chloride is typically preferred.
  • An oral care composition for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a living human or animal, the composition comprising
  • composition having a pH in the range of 6 to 8,
  • the method of treating caries by reducing the lactic acid release comprising the steps of applying the composition being applied to the oral biofilm and/or hard dental tissue according to either of the following application schemes:
  • the quaternary ammonium alkyl component being characterized by the following formula: R-N(CH 2 CH 2 OH)-CH 2 CH 2 CH 2 -N(CH 2 CH 2 OH) 2 * 2HX, with R being selected from C 10-20 alkyl and X being selected from chloride, carbonate, sulphate, phosphate, wherein chloride is typically preferred.
  • An oral care composition for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a living human or animal, the composition comprising
  • quaternary ammonium alkyl component(s) in an amount of at least 0.1 wt.%, and an amino acid selected from
  • glycine in an amount of 0.1 to 10 wt.%; or
  • leucine in an amount of 0.1 to 5 wt.%; or
  • isoleucine in an amount of 0.1 to 5 wt.%; or
  • lysine in an amount of 0.1 to 10 wt.%; or
  • methionine in an amount of 0.1 to 10 wt.%; or
  • phenylalanine in an amount of 0.1 to 5 wt.%; or
  • threonine in an amount of 0.1 tolO wt.%; or
  • valine in an amount of 0.1 to 8 wt.%; or
  • tryptophan in an amount of 0.1 to 2 wt.%; or
  • composition having a pH in the range of 6 to 8,
  • the method of treating caries by reducing the lactic acid release comprising the steps of applying the composition to the oral biofilm and/or hard dental tissue with the aid of an application device selected from a dental tray, mouth guard or clear tray aligner the quaternary ammonium alkyl component being characterized by the following formula: R-N(CH 2 CH 2 OH)-CH 2 CH 2 CH 2 -N(CH 2 CH 2 OH) 2 * 2HX, with R being selected from C 10-20 alkyl and X being selected from chloride, carbonate, sulphate, phosphate, wherein chloride is typically preferred.
  • Embodiment 7 the quaternary ammonium alkyl component being characterized by the following formula: R-N(CH 2 CH 2 OH)-CH 2 CH 2 CH 2 -N(CH 2 CH 2 OH) 2 * 2HX, with R being selected from C 10-20 alkyl and X being selected from chloride, carbonate, sulphate, phosphate, wherein chloride is typically preferred.
  • An oral care composition for use in a method of treating caries or reducing the risk of getting caries by reducing the lactic acid release of lactic acid producing bacteria in an oral biofilm in the mouth of a living human or animal being, the composition comprising quaternary ammonium alkyl component(s) in an amount of at least 0.1 wt.%, water,
  • the ratio of quaternary ammonium alkyl component to water being in a range of 0.005: 100 to 5: 100 with respect to weight
  • composition being applied to the oral biofilm and/or hard dental tissue for at least 2 days,
  • the quaternary ammonium alkyl component being characterized by the following formula: R-N(CH 2 CH 2 OH)-CH 2 CH 2 CH 2 -N(CH 2 CH 2 OH) 2 * 2HX, with R being selected from C 10-20 alkyl and X being selected from chloride, carbonate, sulphate, phosphate, wherein chloride is typically preferred.
  • compositions can be used in a method or therapy described in the present text.
  • TSx Treatment Solutions
  • the Treatment Solutions (TSx) had the following composition:
  • the MCM media containing quaternary ammonium alkyl component had the following composition:
  • the accumulation of lactic acid in the supernatant (0.75 ml PBS-solution with 5% sucrose per each biofilm sample disc) was measured by using the Lactic acid dehydrogenase (LDH)- Nicotinamid- adenin-dinukleotid (NAD)- Phenazine Methosulphate (PMS) - Thiazolyl blue tetrazolium bromide(MTT) - enzyme assay.
  • LDH Lactic acid dehydrogenase
  • NAD Nicotinamid- adenin-dinukleotid
  • PMS Phenazine Methosulphate
  • biofilm samples were washed (in three steps, each lasting 30 sec) in 1.5 ml PBS-solution.
  • the lactic acid was quantified by the lactic acid specific enzyme assay described above. Known correlation between measured enzyme activity and amount of lactic acid was used to translate measured enzyme activity into the amount of lactic acid present in sample of the supernatant.
  • the mass of biofilm was determined as follows: Filter papers were put into 1.5 ml- reaction tubes and weighted. The biofilm, that was grown on the enamel disks was carefully wiped off with the pre-weighted filter papers. The filter papers containing the wet biofilms were placed back into the respective reaction tube and re-weighted. The wet biomass is calculated from the difference in weight. Exposure Procedure 1
  • Bovine sample disks are incubated in a 24 well plate containing 1.8 ml medium in an incubator at 37°C and 60 rpm circular movement. Medium and/ or treatment solutions were added by pipetting and removed by a suction pump.
  • Biofilms were grown in a MCM (mucine containing medium plus 1 % sucrose) human derived saliva biofilm system.
  • biofilms were grown in continuous presence of sucrose and were treated as outlined in the General Procedure above.
  • Bovine sample disks are incubated in a 24 well plate containing 1.8 ml medium in an incubator at 37°C and 60 rpm circular movement. Medium and/or treatment solutions were added by pipetting and removed by a suction pump.
  • Example 2 Human saliva derived biofilm model show reduction in lactic acid release by different concentrations ofN'-Octadecyltrimethylendiamino- N,N,N-tris(2-ethanol)- dihydrochloride
  • biofilms were grown in continuous presence of sucrose and N'- Octadecyltrimethylendiamino-N,N,N'-tris(2-ethanol)-dihydrochloride at 0.005 %, 0.01 %, 0.025 %, 0.01 %, and at 0.1 % according to Exposure Procedure 2.
  • the agar diffusion test is qualitative, easy to perform, and a simple method to determine the antimicrobial activity of diffusive test materials.
  • the methodology includes the inoculation of bacterial cells on nutrient agar Petri dishes and then paper disks containing a defined test substance concentration are placed on the lawn of bacteria. Afterward, the dishes are incubated for 24-48 h at 37°C. After incubation, the diameter of the zone of inhibited growth around the disk is measured (zone of inhibition). The size of the zone of inhibited growth is directly related to the susceptibility of the organism—the larger the zone, the more susceptible the organism is to the antibiotic
  • a 1 : 10 dilution of human saliva with Brain-Heart-Infusion was used to grow on the agar plates. 100 ml of the dilution was applied to the agar plate and was spread equally over the agar surface with a sterile Drigalsky spatula.
  • test solutions had the following composition:

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Abstract

L'invention concerne une composition destinée à être utilisée dans un procédé de traitement de caries par réduction de la libération d'acide lactique de bactéries produisant de l'acide lactique dans un biofilm buccal dans la bouche d'un être humain ou d'un animal, la composition comprenant des composant(s) d'alkyle d'ammonium quaternaire(s) ou des mélanges de ceux-ci dissous ou dispersés dans de l'eau en proportion d'au moins 0,005 % en poids. L'invention concerne également un kit d'éléments destiné à être utilisé dans un procédé de traitement des caries par réduction de la libération d'acide lactique de bactéries produisant de l'acide lactique dans un biofilm buccal dans la bouche d'un être humain ou d'un animal, le kit d'éléments comprenant un élément A comprenant certains composant(s) d'alkyle d'ammonium quaternaire(s) ou des mélanges de ceux-ci, un élément B comprenant de l'eau, et éventuellement un élément C comprenant un dispositif d'application.
EP20721318.2A 2019-04-29 2020-04-23 Composant d'alkyle d'ammonium quaternaire contenant une composition de soin buccal pour le traitement des caries Withdrawn EP3962444A1 (fr)

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JPS5328972B2 (fr) * 1973-12-29 1978-08-17
CH598821A5 (fr) 1974-05-31 1978-05-12 Gaba Ag
CA2272106C (fr) 1996-11-18 2003-11-04 Gaba International Ag Fluorhydrates d'amines
MX2010011647A (es) 2008-04-24 2011-09-30 Gaba Int Holding Ag Composicion para el cuidado oral que comprende estaño disuelto y fluoruro.
US9717667B2 (en) * 2012-12-20 2017-08-01 Colgate-Palmolive Company Oral care composition containing ionic liquids
MX352730B (es) 2012-12-21 2017-12-06 Gaba Int Holding Ag Composición para el cuidado oral.

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