EP3923713A4 - Transgenic mouse models supporting innate immune function - Google Patents

Transgenic mouse models supporting innate immune function Download PDF

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Publication number
EP3923713A4
EP3923713A4 EP20755032.8A EP20755032A EP3923713A4 EP 3923713 A4 EP3923713 A4 EP 3923713A4 EP 20755032 A EP20755032 A EP 20755032A EP 3923713 A4 EP3923713 A4 EP 3923713A4
Authority
EP
European Patent Office
Prior art keywords
innate immune
transgenic mouse
immune function
mouse models
models supporting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20755032.8A
Other languages
German (de)
French (fr)
Other versions
EP3923713A1 (en
Inventor
Leonard D. SHULTZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jackson Laboratory
Original Assignee
Jackson Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jackson Laboratory filed Critical Jackson Laboratory
Publication of EP3923713A1 publication Critical patent/EP3923713A1/en
Publication of EP3923713A4 publication Critical patent/EP3923713A4/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Humanized animals, e.g. knockin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/10—Protein-tyrosine kinases (2.7.10)
    • C12Y207/10001—Receptor protein-tyrosine kinase (2.7.10.1)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • A01K2267/0387—Animal model for diseases of the immune system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals
EP20755032.8A 2019-02-13 2020-02-13 Transgenic mouse models supporting innate immune function Pending EP3923713A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962805257P 2019-02-13 2019-02-13
PCT/US2020/018033 WO2020168029A1 (en) 2019-02-13 2020-02-13 Transgenic mouse models supporting innate immune function

Publications (2)

Publication Number Publication Date
EP3923713A1 EP3923713A1 (en) 2021-12-22
EP3923713A4 true EP3923713A4 (en) 2022-12-14

Family

ID=72044594

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20755032.8A Pending EP3923713A4 (en) 2019-02-13 2020-02-13 Transgenic mouse models supporting innate immune function

Country Status (5)

Country Link
US (1) US20220136002A1 (en)
EP (1) EP3923713A4 (en)
JP (1) JP2022520237A (en)
CN (2) CN117598249A (en)
WO (1) WO2020168029A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230037043A (en) * 2020-07-08 2023-03-15 더 잭슨 래보라토리 Transgenic mouse models supporting human innate immune function
CN116322317A (en) * 2020-07-08 2023-06-23 杰克逊实验室 Transgenic mouse model expressing human HLA-A201 restriction gene
US20240065237A1 (en) * 2020-12-16 2024-02-29 The Jackson Laboratory A genetically modified immunodeficient mouse expressing human or humanized app and mutated human psen1
JP2022101247A (en) * 2020-12-24 2022-07-06 公益財団法人実験動物中央研究所 Human flt3l transgenic rodent family in which human dendritic cell is differentiated

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2817792T3 (en) * 2009-04-03 2021-04-08 Inst Nat Sante Rech Med Mice with a humanized immune system with strengthened dendritic cells
SG176118A1 (en) * 2009-06-29 2011-12-29 Qingfeng Chen Methods of producing humanized non-human mammals
CA3025522A1 (en) * 2016-05-27 2017-11-30 The Board Of Regents Of The University Of Texas System 6-thio-2'-deoxyguanosine (6-thio-dg) results in telomerase dependent telomere dysfunction and cell death in various models of therapy-resistant cancer cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DU YUBIN ET AL: "Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice : Methods and Protocols", PROTEIN CHROMATOGRAPHY : METHODS AND PROTOCOLS, vol. 1874, 2019, New York, NY, pages 139 - 168, XP055977394, ISSN: 1064-3745, ISBN: 978-1-4939-6412-3, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467215/pdf/nihms-1623061.pdf> [retrieved on 20221102], DOI: 10.1007/978-1-4939-8831-0_8 *
DU YUBIN ET AL: "Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice", 24 October 2018 (2018-10-24), pages 1 - 10, XP055977397, Retrieved from the Internet <URL:https://link.springer.com/protocol/10.1007/978-1-4939-8831-0_8> [retrieved on 20221102] *
IWABUCHI RYUTARO ET AL: "Introduction of Human Flt3-L and GM-CSF into Humanized Mice Enhances the Reconstitution and Maturation of Myeloid Dendritic Cells and the Development of Foxp3+CD4+ T Cells", FRONTIERS IN IMMUNOLOGY, vol. 9, 28 May 2018 (2018-05-28), pages 1042, XP055975965, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985304/pdf/fimmu-09-01042.pdf> DOI: 10.3389/fimmu.2018.01042 *
SHANTASHRI VAIDYA ET AL: "Enhanced development of functional human innate immune cells in a novel mouse FLT3null NSG mouse strain expressing human FLT3L", vol. 204, no. 1, Suppl, 1 May 2020 (2020-05-01), pages 223.24, XP009537686, ISSN: 0022-1767, Retrieved from the Internet <URL:https://www.jimmunol.org/content/204/1_Supplement/223.24> *
TIM WILLINGER ET AL: "Improving human hemato-lymphoid-system mice by cytokine knock-in gene replacement", TRENDS IN IMMUNOLOGY, vol. 32, no. 7, July 2011 (2011-07-01), pages 321 - 327, XP028238170, ISSN: 1471-4906, [retrieved on 20110421], DOI: 10.1016/J.IT.2011.04.005 *
YAN LI ET AL: "A novel Flt3-deficient HIS mouse model with selective enhancement of human DC development", EUROPEAN JOURNAL OF IMMUNOLOGY, WILEY-VCH, HOBOKEN, USA, vol. 46, no. 5, March 2016 (2016-03-01), pages 1291 - 1299, XP071227061, ISSN: 0014-2980, DOI: 10.1002/EJI.201546132 *

Also Published As

Publication number Publication date
WO2020168029A1 (en) 2020-08-20
CN113677199A (en) 2021-11-19
CN117598249A (en) 2024-02-27
JP2022520237A (en) 2022-03-29
US20220136002A1 (en) 2022-05-05
EP3923713A1 (en) 2021-12-22

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