EP3887163A4 - Purification alcaline de protéines de soie d'araignée - Google Patents

Purification alcaline de protéines de soie d'araignée Download PDF

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Publication number
EP3887163A4
EP3887163A4 EP19889533.6A EP19889533A EP3887163A4 EP 3887163 A4 EP3887163 A4 EP 3887163A4 EP 19889533 A EP19889533 A EP 19889533A EP 3887163 A4 EP3887163 A4 EP 3887163A4
Authority
EP
European Patent Office
Prior art keywords
spider silk
silk proteins
alkaline purification
alkaline
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19889533.6A
Other languages
German (de)
English (en)
Other versions
EP3887163A1 (fr
Inventor
Phillip MUI
Ritu Bansal MUTALIK
Simon Li
Scott Chan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bolt Threads Inc
Original Assignee
Bolt Threads Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bolt Threads Inc filed Critical Bolt Threads Inc
Publication of EP3887163A1 publication Critical patent/EP3887163A1/fr
Publication of EP3887163A4 publication Critical patent/EP3887163A4/fr
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H1/00—Macromolecular products derived from proteins
    • C08H1/06—Macromolecular products derived from proteins derived from horn, hoofs, hair, skin or leather
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L89/00—Compositions of proteins; Compositions of derivatives thereof
    • C08L89/04—Products derived from waste materials, e.g. horn, hoof or hair
    • C08L89/06—Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin, e.g. gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Insects & Arthropods (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Water Supply & Treatment (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Materials Engineering (AREA)
  • Dermatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Artificial Filaments (AREA)
EP19889533.6A 2018-11-28 2019-11-26 Purification alcaline de protéines de soie d'araignée Pending EP3887163A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862772588P 2018-11-28 2018-11-28
PCT/US2019/063208 WO2020112742A1 (fr) 2018-11-28 2019-11-26 Purification alcaline de protéines de soie d'araignée

Publications (2)

Publication Number Publication Date
EP3887163A1 EP3887163A1 (fr) 2021-10-06
EP3887163A4 true EP3887163A4 (fr) 2022-08-31

Family

ID=70852194

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19889533.6A Pending EP3887163A4 (fr) 2018-11-28 2019-11-26 Purification alcaline de protéines de soie d'araignée

Country Status (6)

Country Link
US (1) US20220017580A1 (fr)
EP (1) EP3887163A4 (fr)
JP (1) JP2022513628A (fr)
KR (1) KR20210096175A (fr)
CN (1) CN114401844A (fr)
WO (1) WO2020112742A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11993068B2 (en) 2022-04-15 2024-05-28 Spora Cayman Holdings Limited Mycotextiles including activated scaffolds and nano-particle cross-linkers and methods of making them

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997008315A1 (fr) * 1995-08-22 1997-03-06 Basel Richard M Procedes de clonage servant a obtenir des proteines de soie d'araignee extremement resistantes
WO2003057720A2 (fr) * 2002-01-11 2003-07-17 Nexia Biotechnologies, Inc. Extraction de proteines filamenteuses a partir de fluides biologiques
US20050261479A1 (en) * 2004-04-29 2005-11-24 Christian Hoffmann Method for purifying and recovering silk proteins using magnetic affinity separation
WO2006008163A2 (fr) * 2004-07-22 2006-01-26 Technische Universitaet Muenchen Proteines de soies d'araignees recombinees
US20070260039A1 (en) * 2002-01-11 2007-11-08 Karatzas Costas N Methods of Producing Silk Polypeptides and Products Thereof
WO2018030499A1 (fr) * 2016-08-10 2018-02-15 Ｓｐｉｂｅｒ株式会社 Procédé de production d'un agrégat insoluble de protéines recombinantes

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016351A1 (fr) * 1990-04-19 1991-10-31 The United States Of America, Secretary Of The Army, The Pentagon Proteines de soie arachneennes recombinees obtenues par genie genetique
US20030013172A1 (en) * 2001-05-14 2003-01-16 Joel Gerendash Novel methods of enzyme purification
US20040132978A1 (en) * 2002-11-12 2004-07-08 Fahnestock Stephen R. Method for purifying and recovering silk proteins in soluble form and uses thereof
CN1259333C (zh) * 2003-05-08 2006-06-14 福建师范大学 制备性基因重组蜘蛛拖丝蛋白的分离纯化方法
ES2799431T3 (es) * 2010-03-31 2020-12-17 Amsilk Gmbh Separación de proteínas objetivo insolubles
US20150047532A1 (en) * 2013-08-13 2015-02-19 Utah State University Synthetic spider silk protein compositions and methods
JP6556122B2 (ja) * 2013-09-17 2019-08-07 ボルト スレッズ インコーポレイテッド 改良シルク繊維を合成するための方法および組成物
KR20220071299A (ko) * 2015-03-16 2022-05-31 볼트 쓰레즈, 인크. 개선된 실크 섬유
EP3263593A1 (fr) * 2016-07-01 2018-01-03 Anna Rising Protéines de soie d'araignée mises au point par génie génétique et leurs utilisations

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997008315A1 (fr) * 1995-08-22 1997-03-06 Basel Richard M Procedes de clonage servant a obtenir des proteines de soie d'araignee extremement resistantes
WO2003057720A2 (fr) * 2002-01-11 2003-07-17 Nexia Biotechnologies, Inc. Extraction de proteines filamenteuses a partir de fluides biologiques
US20070260039A1 (en) * 2002-01-11 2007-11-08 Karatzas Costas N Methods of Producing Silk Polypeptides and Products Thereof
US20050261479A1 (en) * 2004-04-29 2005-11-24 Christian Hoffmann Method for purifying and recovering silk proteins using magnetic affinity separation
WO2006008163A2 (fr) * 2004-07-22 2006-01-26 Technische Universitaet Muenchen Proteines de soies d'araignees recombinees
WO2018030499A1 (fr) * 2016-08-10 2018-02-15 Ｓｐｉｂｅｒ株式会社 Procédé de production d'un agrégat insoluble de protéines recombinantes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LAZARIS A ET AL: "Spider silk fibers spun from soluble recombinant silk produced in mammalian cells", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 295, no. 5554, 18 January 2002 (2002-01-18), pages 472 - 476, XP002243786, ISSN: 0036-8075, DOI: 10.1126/SCIENCE.1065780 *
See also references of WO2020112742A1 *

Also Published As

Publication number Publication date
CN114401844A (zh) 2022-04-26
WO2020112742A1 (fr) 2020-06-04
US20220017580A1 (en) 2022-01-20
JP2022513628A (ja) 2022-02-09
KR20210096175A (ko) 2021-08-04
EP3887163A1 (fr) 2021-10-06

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