EP3842134A1 - Dispositif d'extraction d'acide nucléique - Google Patents

Dispositif d'extraction d'acide nucléique Download PDF

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Publication number
EP3842134A1
EP3842134A1 EP20212678.5A EP20212678A EP3842134A1 EP 3842134 A1 EP3842134 A1 EP 3842134A1 EP 20212678 A EP20212678 A EP 20212678A EP 3842134 A1 EP3842134 A1 EP 3842134A1
Authority
EP
European Patent Office
Prior art keywords
reagent
nucleic acid
extracting device
tube portion
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20212678.5A
Other languages
German (de)
English (en)
Inventor
Chih-Wen Yang
Ruey-Shyan Hong
Tzu-Hui Wu
Ting-Hsuan Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Industrial Technology Research Institute ITRI
Original Assignee
Industrial Technology Research Institute ITRI
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industrial Technology Research Institute ITRI filed Critical Industrial Technology Research Institute ITRI
Publication of EP3842134A1 publication Critical patent/EP3842134A1/fr
Pending legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/07—Stirrers characterised by their mounting on the shaft
    • B01F27/072—Stirrers characterised by their mounting on the shaft characterised by the disposition of the stirrers with respect to the rotating axis
    • B01F27/0724—Stirrers characterised by their mounting on the shaft characterised by the disposition of the stirrers with respect to the rotating axis directly mounted on the rotating axis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/11—Stirrers characterised by the configuration of the stirrers
    • B01F27/112—Stirrers characterised by the configuration of the stirrers with arms, paddles, vanes or blades
    • B01F27/1125—Stirrers characterised by the configuration of the stirrers with arms, paddles, vanes or blades with vanes or blades extending parallel or oblique to the stirrer axis
    • B01F27/11251—Stirrers characterised by the configuration of the stirrers with arms, paddles, vanes or blades with vanes or blades extending parallel or oblique to the stirrer axis having holes in the surface
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/90—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms 

Definitions

  • the tube portion 1222 of the present embodiment has a cylindrical pipe 1222a, and the extension 1242 is provided in the cylindrical pipe 1222a.
  • the extension 1242 has a rectangular cross section, so that the extension 1242 has a first length L in the first direction RD1 and a second length W in the second direction RD2. And the first length L is greater than the second length W.
  • the tube portion 1222 may have a pipe in other cross-sectional shapes, and the extension 1242 may have a cross section in other shapes, so that the gaps between the extension 1242 and the inner wall of the tube portion 1222 are not uniform.
  • the tube portion 1222 has a rectangular pipe, whereas the extension 1242 provided in the rectangular pipe has a circular cross section, a configuration that also includes non-uniform gaps.
  • the present disclosure does not limit the practical shapes thereof.
  • the mixing unit 120 of the present embodiment includes an actuator 126 and a cover 128.
  • the cover 128 covers the chamber portion 1221 of the mixing chamber 122.
  • the stirring assembly 124 further includes a connection 1243.
  • the connection 1243 may pass through the cover 128 and portionally protrude to the outside of the mixing chamber 122 and connects to the actuator 126.
  • the actuator 126 is, for example, a motor, and is adapted to drive the stirring assembly 124 to rotate to perform the stir. In other embodiments, the actuator 126 may be other types of driving devices, and the present disclosure is not limited thereto.
  • the mixing unit 120 may also not include the actuator 126, but there be an actuator included in other external devices to drive the stirring assembly 124 to operate.
  • the specimen in the reagent chamber 110a and the lysis buffer in the reagent chamber 110b flow from the reagent containing unit 110 through the flow channel unit 130 to the mixing chamber 122 by the drive of the pump 160.
  • the heating device 140 heats the mixing chamber 122, and the stirring assembly 124 stirs the specimen and the lysis buffer in the mixing chamber 122, so that cell membranes of the specimen are destroyed by the lysis buffer to precipitate nucleic acid.
  • the binding buffer in the reagent chamber 110c and the magnetic beads in the reagent chamber 110d flow sequentially from the reagent containing unit 110 through the flow channel unit 130 to the mixing chamber 122 by the drive of the pump 160.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP20212678.5A 2019-12-10 2020-12-09 Dispositif d'extraction d'acide nucléique Pending EP3842134A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201962945897P 2019-12-10 2019-12-10

Publications (1)

Publication Number Publication Date
EP3842134A1 true EP3842134A1 (fr) 2021-06-30

Family

ID=74184344

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20212678.5A Pending EP3842134A1 (fr) 2019-12-10 2020-12-09 Dispositif d'extraction d'acide nucléique

Country Status (3)

Country Link
EP (1) EP3842134A1 (fr)
JP (1) JP7101746B2 (fr)
TW (1) TWI742905B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113583807A (zh) * 2021-08-25 2021-11-02 无锡正则精准医学检验有限公司 一种大肠癌检测用核酸提取仪

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006032044A2 (fr) * 2004-09-15 2006-03-23 Microchip Biotechnologies, Inc. Dispositifs microfluidiques
WO2006136999A1 (fr) * 2005-06-23 2006-12-28 Koninklijke Philips Electronics N.V. Dispositif permettant de melanger un milieu liquide
WO2010091080A2 (fr) * 2009-02-03 2010-08-12 Network Biosystems, Inc. Purification d'acide nucléique
WO2014066704A1 (fr) * 2012-10-24 2014-05-01 Genmark Diagnostics, Inc. Analyse cible à multiplexe intégré

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US6374684B1 (en) * 2000-08-25 2002-04-23 Cepheid Fluid control and processing system
GB0227765D0 (en) * 2002-11-28 2003-01-08 Secr Defence Apparatus for processing a fluid sample
JP2008241250A (ja) * 2007-03-23 2008-10-09 Olympus Corp 特異的結合反応を利用した攪拌棒及び該攪拌棒を用いた分析方法並びに分析装置
JP2011128019A (ja) * 2009-12-17 2011-06-30 Hitachi Maxell Ltd マイクロプレートデバイスおよびその利用方法
JP2015159754A (ja) * 2014-02-27 2015-09-07 セイコーエプソン株式会社 核酸増幅方法、核酸抽出用デバイス、核酸増幅反応用カートリッジ、及び核酸増幅反応用キット
CN104059848A (zh) * 2014-06-26 2014-09-24 广州冠科生物科技有限公司 一种核酸提取用混匀装置
CN105441318B (zh) * 2014-08-15 2018-02-13 冠研（上海）专利技术有限公司 配置有阻隔件的核酸产物萃取装置
JP2018502309A (ja) * 2014-11-11 2018-01-25 ジェンマーク ダイアグノスティクス， インコーポレイテッド 閉止サンプル調製・反応システムにおいて、アッセイを実行するための装置及びカートリッジ
WO2016195963A1 (fr) * 2015-05-29 2016-12-08 Tsavachidou Dimitra Procédés de construction de copies de molécules d'acide nucléique reliées de façon consécutive
WO2017223269A1 (fr) * 2016-06-24 2017-12-28 Lonza Ltd. Bioréacteurs à diamètre variable
TWI611171B (zh) * 2016-12-14 2018-01-11 財團法人工業技術研究院 生物樣品處理裝置
TW201903147A (zh) * 2017-06-13 2019-01-16 益生生技開發股份有限公司 核酸萃取方法及裝置
JP2019176831A (ja) * 2018-03-30 2019-10-17 大研医器株式会社 核酸回収方法および容器

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006032044A2 (fr) * 2004-09-15 2006-03-23 Microchip Biotechnologies, Inc. Dispositifs microfluidiques
WO2006136999A1 (fr) * 2005-06-23 2006-12-28 Koninklijke Philips Electronics N.V. Dispositif permettant de melanger un milieu liquide
WO2010091080A2 (fr) * 2009-02-03 2010-08-12 Network Biosystems, Inc. Purification d'acide nucléique
WO2014066704A1 (fr) * 2012-10-24 2014-05-01 Genmark Diagnostics, Inc. Analyse cible à multiplexe intégré

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LIU R H ET AL: "Bubble-induced acoustic micromixing", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, vol. 2, 1 January 2002 (2002-01-01), pages 151 - 157, XP009103055, ISSN: 1473-0197, DOI: 10.1039/B201952C *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113583807A (zh) * 2021-08-25 2021-11-02 无锡正则精准医学检验有限公司 一种大肠癌检测用核酸提取仪

Also Published As

Publication number Publication date
JP2021090420A (ja) 2021-06-17
TW202122566A (zh) 2021-06-16
JP7101746B2 (ja) 2022-07-15
TWI742905B (zh) 2021-10-11

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