EP3797273A1 - Dispositif optique pour un procédé d'imagerie spectroscopique et procédé d'imagerie spectroscopique - Google Patents

Dispositif optique pour un procédé d'imagerie spectroscopique et procédé d'imagerie spectroscopique

Info

Publication number
EP3797273A1
EP3797273A1 EP19726933.5A EP19726933A EP3797273A1 EP 3797273 A1 EP3797273 A1 EP 3797273A1 EP 19726933 A EP19726933 A EP 19726933A EP 3797273 A1 EP3797273 A1 EP 3797273A1
Authority
EP
European Patent Office
Prior art keywords
fiber
illumination light
optical arrangement
core
wavelength
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19726933.5A
Other languages
German (de)
English (en)
Inventor
Gregor Matz
Bernhard Messerschmidt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Grintech GmbH
Original Assignee
Grintech GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Grintech GmbH filed Critical Grintech GmbH
Publication of EP3797273A1 publication Critical patent/EP3797273A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0208—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/00064—Constructional details of the endoscope body
    • A61B1/00071—Insertion part of the endoscope body
    • A61B1/0008—Insertion part of the endoscope body characterised by distal tip features
    • A61B1/00096—Optical elements
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/00163—Optical arrangements
    • A61B1/00165—Optical arrangements with light-conductive means, e.g. fibre optics
    • A61B1/00167—Details of optical fibre bundles, e.g. shape or fibre distribution
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/00163—Optical arrangements
    • A61B1/00172—Optical arrangements with means for scanning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
    • A61B1/0638—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements providing two or more wavelengths
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
    • A61B1/07—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements using light-conductive means, e.g. optical fibres
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/021—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using plane or convex mirrors, parallel phase plates, or particular reflectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0218—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using optical fibers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/10—Scanning systems
    • G02B26/103—Scanning systems having movable or deformable optical fibres, light guides or waveguides as scanning elements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/1086—Beam splitting or combining systems operating by diffraction only
    • G02B27/1093—Beam splitting or combining systems operating by diffraction only for use with monochromatic radiation only, e.g. devices for splitting a single laser source
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/12—Beam splitting or combining systems operating by refraction only
    • G02B27/126—The splitting element being a prism or prismatic array, including systems based on total internal reflection
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/02042—Multicore optical fibres
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/024—Optical fibres with cladding with or without a coating with polarisation maintaining properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/653—Coherent methods [CARS]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/653—Coherent methods [CARS]
    • G01N2021/655—Stimulated Raman
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/08—Optical fibres; light guides
    • G01N2201/0853—Movable fibre optical member, e.g. for scanning or selecting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/08—Optical fibres; light guides
    • G01N2201/0866—Use of GRIN elements

Definitions

  • the application relates to an optical arrangement for a spectroscopic imaging method. Furthermore, the application relates to a spectroscopic imaging method in which the optical arrangement is used.
  • the optical arrangement can be used in particular for a
  • CARS coherent anti-Stokes Raman scattering
  • SRS stimulated Raman scattering
  • Illuminating light of two different wavelengths, the pump and Stokes wavelength are passed through the same optical fiber to the sample.
  • the problem may arise that the
  • Short-pass filter or bandpass filter to put in the beam path, which is optically opaque to the non-resonant ground and passes only the pump and Stokes wavelength to the sample.
  • the CARS signal from the sample can not be collected by the same beam path from the sample to the fiber, which requires a second beam path for the collection of the sample signal with the anti-Stokes wavelength and thus the
  • the invention is in one aspect the object of an optical arrangement for a spectroscopic
  • the optical arrangement for a spectroscopic imaging method comprises a multicore fiber which has at least one first fiber core for guiding a first illumination light and a second fiber core for guiding a second illumination light.
  • the fiber is designed in particular as a double-core fiber and can thus advantageously simultaneously guide the first illumination light and the second illumination light, the first illumination light and the second illumination light
  • Illumination light in particular have different wavelengths.
  • the fiber cores preferably have different diameters and / or different materials. In this way, the Singlemodmaschine and at the same time a good light guide for each wavelength can be ensured.
  • the multi-core fiber has one according to an embodiment of the optical arrangement
  • Fiber scanner for the deflection of the multi-core fiber.
  • Fiber scanner can be designed for example as a piezo scanner. According to an alternative embodiment follows the
  • the mirror scanner can be designed in particular as a MEMS scanner. Through the fiber scanner or the mirror scanner, a time-dependent beam deflection is realized, which allows the imaging.
  • optical arrangement comprises a
  • wavelength-dispersive beam combining element configured to spatially and angularly confine the first illuminating light and the second illuminating light in an object space overlap.
  • the optical arrangement can be part of a microscopic arrangement, in particular the
  • optical arrangement be integrated into an endoscopic probe that is part of a fiber optic endomicroscope.
  • the spectroscopic imaging method for which the optical arrangement is provided may be in particular CARS spectroscopy or SRS spectroscopy.
  • the method two light pulses of the first illumination light and the second illumination light having different wavelengths are simultaneously superimposed on the same location of a sample in the object space.
  • the first fiber core in particular, the first
  • the second illumination light of the Stokes wavelength By separately guiding the pump wavelength and the Stokes wavelength in the two fiber cores, the undesirable four-wave mixing process becomes sufficient
  • Lighting light are in particular of a
  • Vibration vibration of the sample tuned that he coherently drives them and leads to the emission of a third wavelength, the anti-Stokes wavelength, for the
  • wavelength-selective beam combining element between the fiber scanner or the mirror scanner and the object space arranged.
  • the first illumination light and the second illumination light are deflected to scan the object space before passing through the wavelength-selective
  • Beam union element such as a grid or a prism
  • the first illumination light and the second illumination light are deflected by the fiber scanner or the mirror scanner in particular in the same way, wherein the following wavelength-selective
  • Beam union element is advantageously unmoved.
  • a collimating lens is disposed between the fiber scanner or the mirror scanner and the wavelength-selective beam combining element.
  • the collimating lens may be a one-piece or multi-part lens.
  • the collimating lens comprises a gradient index lens (GRIN lens).
  • Lighting light by means of the wavelength-selective Beam unification element it is advantageous if an at least approximate collimation of the illumination lights of both wavelengths before the wavelength-selective
  • Beam union element takes place. From a local
  • an angular offset can be generated.
  • the wavelength-selective beam combination element is advantageously located in the Fourier plane of the imaging optics behind the fiber scanner or mirror scanner, that is, in particular behind the collimating lens.
  • the optical arrangement described here allows a very good diffraction-limited imaging quality for the CARS imaging over a comparatively large field of view based on the overall diameter of the optical arrangement.
  • the multi-core fiber contains a light-conducting jacket for guiding a coming out of the object space
  • Refractive index has as the photoconductive sheath.
  • the multi-core fiber not only the
  • Illumination light in the direction of the object space but advantageously also the object light to be detected in
  • the multi-core fiber has an inner jacket, in which the fiber cores are arranged, wherein the inner jacket is surrounded by the photoconductive jacket.
  • the inner jacket advantageously has a lower refractive index than that
  • Fiber cores and a lower refractive index than the photoconductive sheath on Fiber cores and a lower refractive index than the photoconductive sheath on.
  • a single-mode light-conducting property of the two fiber cores can be achieved by the inner jacket.
  • the inner coat can be
  • the light-conducting in particular be doped with fluorine.
  • Sheath is arranged and has a higher refractive index can be used efficiently for collecting the object light.
  • the fiber cores of the multi-core fiber are preferably made
  • the inner cladding and outer cladding of the multicore fiber may be doped with a dopant
  • the photoconductive jacket preferably comprises pure quartz glass or, for increasing the refractive index, is doped with a dopant suitable thereto, e.g. Doped germanium oxide.
  • the multicore fiber is a polarization-maintaining fiber.
  • the multicore fiber may in particular contain voltage-generating elements which generate a permanent voltage by generating a permanent voltage
  • voltage-generating elements may include
  • the fiber cores are arranged asymmetrically in the multi-core fiber.
  • the fiber cores in this embodiment are arranged asymmetrically to the center of the multi-core fiber.
  • the fiber cores may have different distances from the center of the multi-core fiber.
  • the fiber cores may be arranged one behind the other in the same radial direction as viewed from the center of the multi-core fiber. Depending on how strong this is
  • Illuminating light differs, the object light is deflected by the wavelength-dispersive beam combination element, so that it is not centric on the
  • the collection efficiency of the fiber for the object light can be impaired.
  • the wavelength dispersive beam combining element of the optical arrangement may have various configurations
  • the beam combination element can in particular be a diffraction grating, for example a
  • Reflection diffraction grating or transmission diffraction grating In one embodiment, this is
  • Beam unification element a reflection diffraction grating, so that the optical axis is angled to the object space.
  • the beam combining element may comprise at least one prism or grating prism (GRISM), wherein the
  • Grid prism is a combination of a diffraction grating and a prism.
  • the beam combining element is a multiple prism, the optical axis preferably not changing its direction. It is also possible that the beam combining element a Prism or a multiple prism is and changes the direction of the optical axis in the direction of the object space targeted.
  • the wavelength dispersive beam combining element may be disposed between the fiber scanner or mirror scanner and the object space.
  • Wavelength dispersive beam combining element between the multi-core fiber and the mirror scanner to be arranged Wavelength dispersive beam combining element between the multi-core fiber and the mirror scanner to be arranged.
  • the optical arrangement may be in addition to those hitherto
  • the optical assembly includes
  • the fiber scanner a gradient index lens (GRIN lens), the wavelength-sensitive deflecting element and a front lens group facing the object space, the
  • the optical arrangement includes a spherical achromat, a biconvex lens and a plano-convex lens.
  • the optical arrangement includes a
  • a GRIN lens a deflection prism, a spherical meniscus lens, a spherical achromatic lens, the wavelength-sensitive deflection element and a
  • Front lens group for example, a spherical lens group
  • Achromat, a biconvex lens and a plano-convex lens has.
  • the numerical aperture of the multicore fiber is the numerical aperture of the multicore fiber.
  • the numerical aperture of the optical arrangement to the object space is preferably between 0.2 and 1.1.
  • the optical arrangement has a diameter of less than 5 mm.
  • the small diameter is made possible in particular by the fact that the optical arrangement between the multi-core fiber and the object space only one beam path for the
  • the invention furthermore relates to an endoscopic probe which contains the optical arrangement.
  • Endoscopic probe may be part of a fiber optic endomicroscope, in particular the fiber optic
  • Probe an illumination light source for generating the first and second illumination light and an evaluation unit.
  • Illumination light and the second illumination light are spatially superimposed by a wavelength-dispersive beam combination element in an object space. That from the
  • Object space coming object light is guided in a light-conducting jacket of the multi-core fiber in the direction of an evaluation.
  • the multi-core fiber is advantageously integrated into a fiber scanner which moves the fiber perpendicular to the fiber
  • Discharges exit direction of the light, or the multicore fiber follows a mirror scanner, wherein the object space is scanned by the movement of the fiber scanner or the mirror scanner.
  • the multi-core fiber and the object space advantageously only a single optical Beam path formed in which the first and second
  • Illumination light are guided in the direction of the object space, and in which the object light in the opposite direction to
  • the spectroscopic imaging method may be CARS spectroscopy or SRS spectroscopy, wherein the first illumination light has the pump wavelength and the second illumination light has the Stokes wavelength.
  • 1A is a schematic representation of a cross section through a first example of the multi-core fiber
  • Figure 1B is a schematic diagram of the
  • Figure IC is a schematic diagram of the
  • Figure ID is a schematic representation of a cross section through another example of the multi-core fiber
  • Figures 2 to 10 are each a schematic representation of a cross section through an example of the optical arrangement.
  • optical arrangement and the method according to the principle proposed here are based in particular on
  • FIG. 1A An example of a multi-core fiber 7 is shown in FIG. 1A.
  • FIG. 1B schematically shows the course of the refractive index n over the cross section of the multicore fiber in the direction x, which is shown in FIG. 1A.
  • the multi-core fiber 7 has two fiber cores 1, 2.
  • the first fiber core 1 carries a first illumination light, in particular the light of the pump wavelength for CARS spectroscopy or SRS spectroscopy.
  • the second fiber core 2 carries a second illumination light having a wavelength different from the wavelength of the first illumination light, in particular the light of the Stokes wavelength for CARS spectroscopy or SRS spectroscopy.
  • Both fiber cores 1, 2 preferably have different diameters or materials in order to ensure singlemodidity and, at the same time, good light guidance for the respective wavelength.
  • the fiber cores 1, 2 are made of undoped quartz glass, this reduces unwanted multiphoton intrinsic fluorescence in the fiber and thus ensures better contrast, for example, for multiphoton fluorescence microscopy.
  • the photoconductive jacket 4 can be efficiently used for integrally collecting the object light, for example the CARS, SHG or fluorescence signal of a sample, which is generated in a non-linear imaging process.
  • the photoconductive jacket 4 is surrounded by an outer jacket 5, which has a lower refractive index than the
  • the multi-core fiber 7 is preferably one
  • a polarization maintaining fiber is advantageous for a nonlinear imaging process because the use of polarized light minimizes the required peak intensity and thus reduces damage to the object to be examined.
  • polarization-maintaining property of the multi-core fiber 7 can in particular by the insertion of voltage-generating Elements 6 can be achieved, which is an asymmetric
  • Figure IC shows the refractive index profile in one
  • Cloak 3 The light pipe in the fiber cores 1, 2 is realized by the higher refractive index with respect to the photoconductive jacket 4, which is realized for example with the aid of a dopant such as germanium.
  • Multi-core fiber 7 shown.
  • the two fiber cores 1, 2 are arranged asymmetrically in the multi-core fiber 7, in particular eccentrically to the photoconductive
  • Beam union element 12 is deflected so that it does not hit the fiber end face centric.
  • spectroscopic imaging method is shown in FIG.
  • the spectroscopic imaging method for example, the light of a
  • Multi-core fiber 7 coupled.
  • the multi-core fiber 7 emits the light of the two wavelengths with a certain NA and a spatial offset corresponding to the distance between the first fiber core 1 and the second fiber core 2.
  • a subsequent collimating lens 11 leads to a
  • Beam union element 12 is spatially and angularly superimposed.
  • a subsequent front lens group 13 now focuses the beams of the illumination light with a sufficiently high NA in the object space 14 to those for the
  • Object space generated to be detected object light can
  • the object light is returned in the optical arrangement on the same beam path and integrallyassemblingsamme11 of the photoconductive jacket of the multi-core fiber 7.
  • the distal end of multicore fiber 7 is provided with a fiber scanner (not shown) in the example of Figure 2 to deflect the multicore fiber.
  • a fiber scanner (not shown) in the example of Figure 2 to deflect the multicore fiber.
  • the object space 14 is scanned in accordance with the magnification of the optical arrangement.
  • coincident movement of the photoconductive jacket of the multi-core fiber 7 it functions as a quasi-confocal optical detector for the signal emitted by the sample in volume around the excitation spot.
  • the photoconductive sheath 4 of the multi-core fiber 7 is designed, the confocality can be influenced. For a high
  • the object light of the sample to be detected differs in wavelength from the wavelengths of the illumination light guided in the fiber cores 1, 2, the object light is also deflected by the wavelength-dispersive beam combination element 12 so that it does not strike the fiber end face centrically. Thereby, the collection efficiency of the multi-core fiber 7 for the object light can be impaired. Therefore, it may be advantageous, the area of the two fiber cores 1 and 2 eccentrically to
  • a photomultiplier tube For imaging, for example, a photomultiplier tube (PMT) or a spectrometer, which in coordination with the
  • Stimulation signal is triggered at the proximal end of the
  • Multi-core fiber 7 can be used as a detector of the light emitted by the sample. It is advantageous that no second beam path in the optical arrangement is necessary to collect the object light, and also no cleaning filter in the optical arrangement must be used, as the
  • FIG. 7 A second example of the optical arrangement is shown in FIG.
  • the multicore fiber 7 follows a collimation unit 8 and a mirror scanner 9.
  • the mirror scanner 9 is a MEMS mirror scanner.
  • Wavelength-dispersive beam combination element 12 and the front lens group 13 is passed into the object space 14.
  • the first and second illumination light in particular the light of
  • Beam union element 12 spatially and angularly united.
  • the beam combining element 12 may be a linear diffraction grating.
  • the position of the beam combining element 12 can be chosen differently in this design of the optical arrangement since there are two Fourier planes in this arrangement.
  • the beam combination element 12 can be arranged correspondingly either directly after the collimation unit 8 or after the further collimation unit 11.
  • FIG. 4 shows a further example of the optical arrangement.
  • the collimating unit 11 is designed as a GRIN lens and collimates in the optical arrangement the illumination light, which emerges as a fiber scanner
  • Beam combining element 12 which is for example a linear transmission diffraction grating and a
  • Wavelength-dependent diffraction angle generated spatially and angularly superimposed.
  • chromatic and other aberrations over the field-correcting front lens group 13, which consists of, for example, an achromatic lens and two spherical singlet lenses, focuses the light with a numerical aperture of, for example, about 0.54 into the object space 14 in which it is focused the spectroscopic imaging,
  • non-linear CARS process comes on a sample.
  • an NA of at least 0.15 is advantageous in order to ensure in particular the condition of momentum conservation.
  • the generated signal is subsequently returned to the same path
  • Beam union element 12 can be found:
  • grating period g (f * dl) / a.
  • the grating period g is given in ym per line, f is the focal length of the collimation unit 11,
  • Dl corresponds to the wavelength difference between the pump and Stokes wavelengths
  • a corresponds to the distance between the centers of the two fiber cores 1, 2.
  • FIG. 5 shows a further example of the optical arrangement in which the wavelength-dispersive
  • Beam union element 12 is realized by a two-component prism.
  • the prism consists of a crown and a flint glass and is designed to produce the required wavelength-selective angular offset while maintaining the direction of the optical axis.
  • FIG. 6 and FIG. 7 show further examples of the optical arrangement, which are essentially analogous to those of FIGS.
  • the multicore fiber 7 is fastened to a GRIN lens functioning as a collimation unit 8.
  • a prism By means of a prism, a 90 ° deflected, collimated beam of the
  • Illumination light is scanned by a MEMS mirror scanner 9 and focused into an intermediate image by a lens group 10 that corrects for chromatic aberration.
  • a lens group 10 that corrects for chromatic aberration.
  • FIG. shows the possibility of an angled measurement using a prism as Wavelength dispersive beam combining element 12.
  • beam deflection by the prism is 35 degrees.
  • the collimating unit 11 in this example is a lens group consisting of a GRIN lens and a doublet lens, and the front lens group 13 is formed of two singlet lenses.
  • the collimating unit 11 may be a lens group consisting of a GRIN lens and a lens
  • Doublet lens, and the front lens group 13 is formed of two singlet lenses.
  • the wavelength dispersive beam combining element 12 in this example is a linear reflection diffraction grating disposed at 45 degrees to the optical axis and, for example, one
  • Fig. 10 is an example of the optical arrangement
  • the beam combining element 12 is a grating prism (GRISM) consisting of a grating prism
  • Wavelength dispersive beam combining element 12 as in the examples of Figures 4 and 6, or a double prism 12, as in the examples of Figures 5 and 7, or by the combination of a grid and a prism as in the example of Figure 10 possible.
  • the optical axis may also be advantageous for the optical axis to be tilted within the optical arrangement, for example in the case of an endoscopic probe which is laterally oriented
  • wavelength-dispersive beam combining element 12 as
  • Reflection diffraction grating as in the example of Figure 9, to realize, or by a deflection prism, as in the example of Figure 8, to realize.

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Abstract

L'invention concerne un dispositif optique pour un procédé d'imagerie spectroscopique, comprenant une fibre multicoeur (7) qui comprend au moins un premier coeur de fibre (1) pour guider une première lumière d'éclairage, un deuxième coeur de fibre (2) pour guider une deuxième lumière d'éclairage, et un élément de convergence de faisceau (12) dispersif en longueur d'onde qui est conçu pour superposer spatialement la première lumière d'éclairage et la deuxième lumière d'éclairage dans un espace objet (14). L'invention concerne en outre un procédé d'imagerie spectroscopique utilisant le dispositif optique.
EP19726933.5A 2018-05-22 2019-05-20 Dispositif optique pour un procédé d'imagerie spectroscopique et procédé d'imagerie spectroscopique Pending EP3797273A1 (fr)

Applications Claiming Priority (2)

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DE102018112253.5A DE102018112253A1 (de) 2018-05-22 2018-05-22 Optische Anordnung für ein spektroskopisches bildgebendes Verfahren und spektroskopisches bildgebendes Verfahren
PCT/EP2019/062954 WO2019224146A1 (fr) 2018-05-22 2019-05-20 Dispositif optique pour un procédé d'imagerie spectroscopique et procédé d'imagerie spectroscopique

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EP3797273A1 true EP3797273A1 (fr) 2021-03-31

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WO2022035707A1 (fr) * 2020-08-08 2022-02-17 Pavilion Integration Corporation Fibres multicœurs, leurs procédés de préparation et leur utilisation
DE102021102092A1 (de) * 2021-01-29 2022-08-04 Leibniz-Institut für Photonische Technologien e.V. (Engl.Leibniz Institute of Photonic Technology) Hybrid-Lichtleitfaser, Endoskopisches System und Verfahren zum Untersuchen einer Probe

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US6697192B1 (en) * 2000-11-08 2004-02-24 Massachusetts Institute Of Technology High power, spectrally combined laser systems and related methods
US20050038322A1 (en) * 2003-08-11 2005-02-17 Scimed Life Systems Imaging endoscope
US7430352B2 (en) * 2005-07-29 2008-09-30 Aculight Corporation Multi-segment photonic-crystal-rod waveguides for amplification of high-power pulsed optical radiation and associated method
US7414729B2 (en) * 2005-10-13 2008-08-19 President And Fellows Of Harvard College System and method for coherent anti-Stokes Raman scattering endoscopy
US8526110B1 (en) * 2009-02-17 2013-09-03 Lockheed Martin Corporation Spectral-beam combining for high-power fiber-ring-laser systems
DE102009011647B4 (de) 2009-03-04 2020-02-20 Carl Zeiss Meditec Ag CARS-Endoskop
US8582096B2 (en) * 2009-12-18 2013-11-12 The Regents Of The University Of California System and method for efficient coherence anti-stokes raman scattering endoscopic and intravascular imaging and multimodal imaging
US9146346B2 (en) * 2013-01-31 2015-09-29 Institut National D'optique Optical fiber for Coherent Anti-Stokes Raman scattering endoscopes
CN110251058B (zh) * 2014-07-24 2022-10-04 Z思快尔有限公司 多芯纤维内窥镜
US9145346B1 (en) 2014-12-10 2015-09-29 Eastman Chemical Company Process for 2,2,4,4-tetramethylcyclobutane-1,3-diol crystallization
US20160357007A1 (en) 2015-05-05 2016-12-08 Eric Swanson Fixed distal optics endoscope employing multicore fiber
DE102016003334A1 (de) 2016-03-14 2017-09-14 Universität Stuttgart (Körperschaft Des Öffentlichen Rechts) Anordnung und Verfahren zur Raman-Spektroskopie, insbesondere auch zur Tumorgewebe- und Aorta-Diagnostik
US10898068B2 (en) * 2016-11-01 2021-01-26 Canon U.S.A., Inc. Multi-bandwidth spectrally encoded endoscope

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US20210215535A1 (en) 2021-07-15
DE102018112253A1 (de) 2019-11-28
US11448551B2 (en) 2022-09-20

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