EP3784040A1 - Procédé de lutte contre le fusarium sur la pomme de terre à l'aide de naphtalène à alkyle inférieur - Google Patents

Procédé de lutte contre le fusarium sur la pomme de terre à l'aide de naphtalène à alkyle inférieur

Info

Publication number
EP3784040A1
EP3784040A1 EP19792929.2A EP19792929A EP3784040A1 EP 3784040 A1 EP3784040 A1 EP 3784040A1 EP 19792929 A EP19792929 A EP 19792929A EP 3784040 A1 EP3784040 A1 EP 3784040A1
Authority
EP
European Patent Office
Prior art keywords
naphthalene
lower alkyl
dmn
dimethyl
potato
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19792929.2A
Other languages
German (de)
English (en)
Other versions
EP3784040A4 (fr
Inventor
Michael A. CAMPBELL
Addie Waxman
John M. Forsythe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
1 4 Group Inc
Original Assignee
1 4 Group Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 1 4 Group Inc filed Critical 1 4 Group Inc
Publication of EP3784040A1 publication Critical patent/EP3784040A1/fr
Publication of EP3784040A4 publication Critical patent/EP3784040A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons

Definitions

  • Embodiments of the present disclosure relate to methods of controlling Fusarium on potatoes. More particularly, this invention relates to methods of controlling Fusarium on potatoes using lower naphthalene compounds, such as l,4-dimethylnaphthalene (l,4-DMN).
  • lower naphthalene compounds such as l,4-dimethylnaphthalene (l,4-DMN).
  • Fusarium infection on potato has become increasingly widespread in many potato-growing regions, and it often leads to 30-50% yield losses and decreased tuber quality. Fusarium is one of the most important yield-limiting diseases in potato production worldwide. Fusarium (sometimes referred to as“fusarium wilt”) can be caused by a complex of Fusarium species, including F. eumartii, F. avenaceum, F. solani, F. graminearum, F. sambucinum, and F. oxysporum.
  • Fusarium spp. naturally thrive in most soils across the globe, making it casual inhabitant of potato tuber’s superficial microbiome. Consequently, exposure of tuber flesh to fusarium can result in a detrimental vascular disease and can lead to a significant loss in harvest during tuber storage and processing. Fusarium spp. can survive for years in field soil, but the primary inoculum is generally home on tuber surfaces. Fusarium spp. infect potatoes via wounds in the periderm inflicted during harvesting or subsequent handling. In stored potatoes, Fusarium infection develops most rapidly in high relative humidity (>70%) and at l5°C-20°C, but continues to advance at the coldest temperatures safe for potatoes.
  • F. Ayed et al. report that the treatment of potato tuber with two known fungicides (fludioxonil and quinoline) showed a limited effect in controlling Fusarium wilt development. See Fakher Ayed et al,“ Evaluation of fungicides for control of Fusarium wilt of potato”, Plant Pathology Journal. 2006, 5(2), 239-243).
  • U.S. Publication No. 2011/0047654 discloses the use of antifungal composition comprising natamycin and at least one phosphite containing compound for treating potatoes against fungus selected from of Mycosphaerella musicola, Mycosphaerella fljensis, Fusarium oxysporum, Alternaria solani, or Alternaria alternata.
  • U.S. Patent No. 5,552,315 discloses the use of specific strains of gram-negative bacteria to suppress (i.e., reduce the incidence or severity) fungal-induced potato diseases under storage conditions.
  • Thiabendazole and 2-aminobutane fungicides have been used to control Fusarium by application to potato tubers at harvest or at pre-planting.
  • strong concerns are being raised about the potential adverse impact of these chemicals on ground and surface water reservoirs and on the health of agricultural product workers and consumers.
  • FIG. 1 is a schematic diagram showing the evaluation of 1, 4-dimethyl naphthalene (1,4- DMN) for controlling F. oxysporum extracted from the surface of potato tubers;
  • FIG. 2 shows the primers used for amplification of fungal DNA;
  • FIG. 3 shows the NCBI BLASTn sequence alignments to PCR Products
  • FIG.4 is the fungal stain using Lactophenol Cotton Blue (1000X).
  • FIG. 5 is the NMR spectrum confirming that the treatment is l,4-DMN.
  • Embodiments of the present disclosure relate to methods of controlling Fusarium infections on potato, wherein the methods comprise contacting potato tuber with a composition comprising lower alkyl naphthalene.
  • the potato tuber treated with such composition is less susceptive to Fusarium infection as compared to untreated potato tuber.
  • Further embodiments of the present disclosure relate to methods of controlling the growth of Fusarium oxysporum on potato tuber, wherein the methods comprise contacting the potato tuber with a composition comprising a lower alkyl naphthalene.
  • the potato tuber may be contacted with a composition comprising lower alkyl naphthalene lower, before or during the storage, during shipment of potato tuber, or prior to planting.
  • the composition comprising lower alkyl naphthalene may be in the form of liquid composition.
  • lower alkyl naphthalene as used herein includes a naphthalene compound having at least one C2-C12 alkyl substituents.
  • alkyl as used herein includes a linear alkyl group, a branched alkyl group, or a cyclic alkyl group.
  • Non-limiting examples of lower alkyl naphthalenes suitable for the present disclosure are: dimethyl naphthalene (DMN) such as 1, 2-dimethyl naphthalene (l,2-DMN), 1, 4-dimethyl naphthalene (l,4-DMN), 1, 6-dimethyl naphthalene (l,6-DMN), and diisopropyl naphthalene (DIPN); or trimethyl naphthalene.
  • the liquid composition may comprise a mixture of various lower alkyl naphthalenes.
  • the lower alkyl naphthalene is dimethyl naphthalene.
  • the lower alkyl naphthalene is 1, 4-dimethyl naphthalene (l,4-DMN), which is a compound naturally abundant in the tissues of dormant potatoes.
  • DN dimethyl naphthalene
  • the composition comprising DMN (such as l,4-DMN) can readily be applied to the potato storage units as an aerosol, which forms a vapor and is adsorbed by the stored potatoes.
  • the liquid composition comprising DMN (such as l,4-DMN) may be sprayed on freshly dug potatoes in the field immediately after being dug, during transport from the field, while being conveyed into a storage facility, or immediately after being stored.
  • the potatoes may also be treated with a vapor or mist of DMN (such as l,4-DMN) either in addition to initial treatments or instead of earlier treatments.
  • the liquid composition may further comprise at least one of the following chemicals: adjuvants, solvents/diluents, surfactants, lubricants, odorants, deodorants, fungicides, biocides, or pesticides.
  • Diluents depending upon their type and/or aroma characteristics, may be used in varying amounts.
  • suitable diluents may include: C2-C12 alcohols (e.g., methanol, ethanol, isopropyl alcohol, hexanol, octanol, nonanol, decanol and dodecanol, and the like); lower alkyl esters of C6-C10 aliphatic alcohols (e.g., methyl, ethyl or propyl esters of l-nonanol or l-hexanol); aliphatic aldehydes; or aliphatic ketones.
  • the liquid composition comprises a solvent/diluent along with the dimethyl naphthalene (DMN), wherein the ratio of solvent/diluent to DMN is from about 2: 1 to about 50: 1.
  • DN dimethyl naphthalene
  • the liquid composition comprising lower alkyl naphthalene may be applied to the potato tuber by various techniques including, but not limited to, the following: immersing the potato tuber in a bath of the liquid composition; spraying the liquid composition to the potato tuber; or applying the liquid composition as a mist over the potato tuber.
  • the liquid composition is an aqueous composition.
  • Lower alkyl naphthalene e.g., DMN
  • DMN lower alkyl naphthalene
  • the mixing may be conducted in a tank as in a mixing chamber of a spray or misting machine.
  • Treating potato tuber with a composition comprising DMN greatly impacts the microbiome on the surface of potatoes, especially with regards to species diversity.
  • DMN such as l,4-DMN
  • F. oxysporum a particular strain of pathogenic fungus responsible for the Fusarium wilt disease.
  • F. oxysporum was isolated from the surface of potato tuber using the Fusarium selective media Nash-Snider, to which cultures were transferred and maintained on nutritionally complex potato dextrose agar plates.
  • DNA sequencing using selective primers NL1, NL2, ITS1F, and ITS2R confirmed the fungus to be F. oxysporum.
  • a single spore isolation technique was utilized to record the number and growth response of the spores treated with l,4-DMN in comparison with the spores treated with water (i.e., the Control).
  • Single spore isolate plates were contained in vacuum sealed chambers and incubated at a constant temperature of about 22°C, while exposed to 7.5pl of l,4-DMN via aerosol dispersion for four days. After a day of rest, the spores on each plate were measured and counted. As shown in Figure 1 and Table 1, the F. oxysporum spores treated with l,4-DMN showed slower mycelium diameter growth than the untreated F. oxysporum spores (i.e., the Control).
  • DMN (such as l,4-DMN) can be used to control (e.g., reduce the incidence or severity) the growth of F. oxysporum, which is an exemplary Fusarium species that cause the Fusarium disease on potatoes.
  • Figure 1 is a schematic diagram, showing the evaluation of l,4-DMN for controlling F. oxysporum extracted from the surface of potato tuber.
  • Potato skin scrapings were cultured on the Fusarium selective media Nash Snider Agar, and later transferred and maintained on nutritionally complex Potato Dextrose Agar (PDA) (Step 1.1 in Figure 1). DNA isolations were performed on single spore isolates using a Power Soil DNA Isolation Kit (Step 1.2 in Figure 1). Several primers as shown in Figure 2 were studied for amplification of Fungal DNA. Fusarium selective primers NL1, NL2, ITS1F, and ITS2 allowed PCR amplification of fungal strains genomically similar to Fusarium. DNA from samples showed strong PCR product bands via gel electrophoresis (Step 1.3 in Figure 1).
  • F. oxysporum successfully isolated from the tuber surface showed inhibition of mycelium growth after exposure to l,4-DMN for four days.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne un procédé de lutte contre la fusariose de la pomme de terre, le procédé comprenant l'étape consistant à mettre en contact un tubercule de pomme de terre avec une composition comprenant du naphtalène à alkyle inférieur. Le développement du Fusarium est contrôlé par traitement du tubercule de pomme de terre avec une composition comprenant du naphtalène à alkyle inférieur.
EP19792929.2A 2018-04-26 2019-04-26 Procédé de lutte contre le fusarium sur la pomme de terre à l'aide de naphtalène à alkyle inférieur Withdrawn EP3784040A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862663161P 2018-04-26 2018-04-26
PCT/US2019/029462 WO2019210245A1 (fr) 2018-04-26 2019-04-26 Procédé de lutte contre le fusarium sur la pomme de terre à l'aide de naphtalène à alkyle inférieur

Publications (2)

Publication Number Publication Date
EP3784040A1 true EP3784040A1 (fr) 2021-03-03
EP3784040A4 EP3784040A4 (fr) 2021-12-29

Family

ID=68295806

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19792929.2A Withdrawn EP3784040A4 (fr) 2018-04-26 2019-04-26 Procédé de lutte contre le fusarium sur la pomme de terre à l'aide de naphtalène à alkyle inférieur

Country Status (10)

Country Link
EP (1) EP3784040A4 (fr)
CN (1) CN112469276A (fr)
AR (1) AR114844A1 (fr)
AU (1) AU2019260779B2 (fr)
BR (1) BR112020021676A2 (fr)
CA (1) CA3097909A1 (fr)
EA (1) EA202092565A1 (fr)
MX (1) MX2020011328A (fr)
TW (1) TW201945326A (fr)
WO (1) WO2019210245A1 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA720769A (en) * 1965-11-02 John D. Douros, Jr. Alkylated naphthalene fungal growth inhibitors
US3361545A (en) * 1962-01-15 1968-01-02 Sun Oil Co Sludge inhibiting jet fuel additives and methods for their use
FR2703880B1 (fr) * 1993-04-15 1995-06-02 Roussel Uclaf Utilisation de dérivés de l'acide 7-éthynyl alpha-(méthoxy-méthylène) 1-naphtalène acétique pour la préparation de compositions fongicides.
WO2014112997A1 (fr) * 2013-01-16 2014-07-24 1,4 Group, Inc. Traitement des cultures à naphtalène alkyle inférieur pour modifier le cycle cellulaire et la régulation d'eau

Also Published As

Publication number Publication date
WO2019210245A1 (fr) 2019-10-31
AU2019260779A1 (en) 2020-11-26
AR114844A1 (es) 2020-10-21
EA202092565A1 (ru) 2021-02-20
TW201945326A (zh) 2019-12-01
EP3784040A4 (fr) 2021-12-29
AU2019260779B2 (en) 2022-05-26
CN112469276A (zh) 2021-03-09
CA3097909A1 (fr) 2019-10-31
MX2020011328A (es) 2021-04-12
BR112020021676A2 (pt) 2021-04-06

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