EP3698872B1 - Mikrofluidischer detektions-chip zur mehrkanal-schnelldetektion - Google Patents

Mikrofluidischer detektions-chip zur mehrkanal-schnelldetektion Download PDF

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Publication number
EP3698872B1
EP3698872B1 EP19819952.3A EP19819952A EP3698872B1 EP 3698872 B1 EP3698872 B1 EP 3698872B1 EP 19819952 A EP19819952 A EP 19819952A EP 3698872 B1 EP3698872 B1 EP 3698872B1
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Prior art keywords
microfluidic
detection
channel
chip
bottom plate
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EP19819952.3A
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English (en)
French (fr)
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EP3698872A1 (de
EP3698872A4 (de
Inventor
Xingshang XU
Jeffery Chen
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Nanjing Lanyu Biological Technology Co Ltd
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Nanjing Lanyu Biological Technology Co Ltd
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements

Definitions

  • the present invention relates to the technical field of medical devices, and in particular, to a microfluidic detection chip for multi-channel rapid detection.
  • Microfluidics is a technology applied across a variety of disciplines including engineering, physics, chemistry, microtechnology, and biotechnology. Microfluidics involves the study of trace fluids and the study of how to manipulate, control, and use such small amounts of fluids in various microfluidic systems and devices such as microfluidic chips.
  • microfluidic biochips referred to as "lab-on-chips" are used to integrate test operations in the field of molecular biology for purposes such as analyzing enzymes and DNA, detecting biochemical toxins and pathogens, and diagnosing diseases.
  • the microfluidic chip is a hot area in the development of current miniaturized total analysis systems.
  • Microfluidic chip analysis takes a chip as an operating platform, analytical chemistry as the basis, micro-electromechanical processing technology as the support, a micro-pipeline network as a structural feature, and life sciences as the main application object at present, and is the focus of the development of the current miniaturized total analysis system field.
  • the microfluidic chip analysis aims at integrating the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc. on the microchip.
  • the microfluidic chip is the main platform for microfluidic technology implementation.
  • Device features of the microfluidic chip are mainly that the effective structures (channels, detection chambers and some other functional components) containing fluids are micron-scale-sized in at least one dimension. Due to the micron-scale structure, the fluid shows and produces special performance different from the macro-scale. As a result, unique analytical performance has been developed. Characteristics and development advantages of the microfluidic chip: the microfluidic chip has the characteristics of controllable liquid flow, minimal consumption of samples and reagents, and ten to hundreds of times improvement in analysis speeds. Simultaneous analysis of hundreds of samples can be performed in minutes or even less, and the entire process of sample pretreatment and analysis can be realized online. The application purpose of the microfluidic chip is to realize the ultimate goal of the miniaturized total analysis systems, i.e., the lab-on-chip. The key application field of current work development is the field of life sciences.
  • Cidadic chip comprising a glass substrate layer, an intermediate layer, and an upper cover layer sequentially stacked from bottom to top.
  • the glass substrate layer, the intermediate layer, and the upper cover layer cooperate to define a closed annular microfluidic channel and detection chambers.
  • the microfluidic channel is located outside the detection chambers and communicated with the detection chambers.
  • a fluid injection port communicated with the microfluidic channel is formed on one side of the upper cover layer.
  • a plurality of exhaust holes are formed on the upper cover layer at the other end of the microfluidic channel.
  • US patent application US2008297169 describes a device, test cards, methods and kits which are useful for determining the particle fraction and rate of viscosity of a fluid sample, the presence of an analyte in a fluid sample, or the aggregation of particles in a fluid sample to detect an analyte or as an immunologic assay.
  • US Patent application US2007158246 describes a coagulation detecting apparatus having a structure defining a container for a fluid, and containing particles for movement through the container under the influence of a magnetic field.
  • a magnetic arrangement provides sequential magnetic fields to the container such as to cause the particles to move.
  • a light source illuminates the container and a detector detects optical radiation of the light source after passing through the container, the detector being arranged for optically detecting at least one of presence of the particles at a predetermined location in the fluid and movement of the particles through a predetermined location in the fluid.
  • the technical problem to be solved by the present invention is to provide a microfluidic detection chip for multi-channel rapid detection with a reasonably designed sample inlet to avoid sample contamination, large detection throughout, and high detection efficiency and accuracy.
  • a microfluidic detection chip for multi-channel rapid detection comprising a chip body, a chip sampling port, a plurality of independent detection chambers, and a microfluidic channel being disposed on the chip body.
  • the chip sampling port is communicated with the detection chambers by means of the microfluidic channel.
  • the chip body further comprises an electrode.
  • the detection chambers are connected to the electrode.
  • the microfluidic channel comprises a main flow channel and a plurality of branch microfluidic channels, a tail end of the main flow channel is divided into the plurality of branch microfluidic channels, and the plurality of branch microfluidic channels are communicated with the plurality of independent detection chambers in a one-to-one correspondence manner.
  • the other end of the main flow channel is communicated with the chip sampling port.
  • the microfluidic chip has the characteristics of high accuracy, fast speed, and low detection cost in detection, and thus is suitable for detection in the link of precision medicine.
  • the main flow channel and the plurality of branch microfluidic channels in a specific structural form to guide the flow of blood samples, one sample chamber can simultaneously inject samples into a plurality of reaction chambers without contaminating the samples, and it is easy to inject samples.
  • the samples After sampled by the chip sampling port, the samples simultaneously flow through the main flow channel to the plurality of branch microfluidic channels, and then flow into the plurality of independent detection chambers, where detection reagents are embedded in advance, so that the plurality of samples can be simultaneously detected, and the multi-channel effect is achieved.
  • the chip is simple in structure and convenient in operation, thereby improving the detection efficiency, greatly reducing the consumption of resources, realizing rapid detection, and lowering the cost.
  • the chip body comprises a bottom plate layer, an intermediate layer, and an upper cover layer in sequence from bottom to top.
  • the bottom plate layer, the intermediate layer, and the upper cover layer cooperate to define a closed microfluidic channel and a plurality of independent detection chambers.
  • the microfluidic channel and the detection chambers are located in the intermediate layer.
  • a liquid injection port and a plurality of exhaust holes are formed on the upper cover layer, the plurality of exhaust holes are provided on one side of the upper cover layer corresponding to the tail end of the microfluidic channel, and the liquid injection port is communicated with a front end of the microfluidic channel.
  • An electrode is provided on the bottom plate layer, and the detection chambers are connected to the electrode.
  • the chip with a three-layer structure of the bottom plate layer, the intermediate layer and the upper cover layer has a reasonable design, a simple and compact structure, and reduced cost, and has a chip sampling port for easy injection of samples.
  • a plurality of exhaust holes are formed on the upper cover, so that the flow resistance of the fluid to be detected is reduced, and the flow is faster, thereby realizing rapid filling of the detection chambers.
  • the provision of the exhaust holes facilitates the flow of the samples and thus the sample injection. If there is no exhaust hole, the sample cannot flow into the detection chamber for reaction.
  • the detection reagents are embedded in the detection chambers of the chip in advance.
  • the plurality of independent detection chambers are distributed in a fan shape, and the tail end of the main flow channel is divided into a plurality of branch microfluidic channels, and the plurality of branch microfluidic channels are then communicated with the plurality of independent detection chambers.
  • a notch is formed on one side of a lower end of the bottom plate layer.
  • the liquid injection port, the funnel region, and the notch are respectively formed at corresponding positions on the upper cover layer, the intermediate layer, and the bottom plate layer and have different sizes.
  • the chip sampling port is formed by the liquid injection port, the funnel region, and the notch and is connected to the bottom of the detection chambers by means of the microfluidic channel.
  • the chip sampling port is set to a funnel shape with a large bottom plate area, a small upper cover area and a funneled intermediate layer. This structure is reasonable and simple, making the sample easily flow in without being contaminated and improving the detection efficiency.
  • a further improvement of the present invention is that: the liquid injection port, the funnel region, and the notch are all arc-shaped and have different radians; the liquid injection port and the funnel region are semicircular arc-shaped, and the radius of the funnel region is not less than the arc radius of the liquid injection port; a curved main flow channel in the funnel region is divided into a plurality of branch microfluidic channels which are communicated with the plurality of independent detection chambers in a one-to-one correspondence manner; the area of the notch is smaller than the area of the funnel region; or the main flow channel is a funnel region, the liquid injection port is arc-shaped and overlaps with a part of the funnel region, the funnel region is converged inward from an opening to form a horn shape, and the funnel region is inwardly divided into a plurality of branch microfluidic channels at the tail end thereof, and the plurality of branch microfluidic channels are connected to the plurality of independent detection chambers in a one-to-one correspondence manner.
  • the liquid injection port is semicircular arc-shaped. Under the condition of the same area, such a structure provides the largest number of injected samples, and the radius of the funnel region is not less than the arc radius of the liquid injection port, so that the funnel region can fully accommodate the sample liquid injected from the liquid injection port, without sample loss.
  • the curved flow channel is provided so that the samples slowly flow into the detection chambers, without causing a sudden increase in the atmospheric pressure of the detection chambers.
  • the liquid injection port is set to an arc shape, and overlaps with a part of the funnel region; the funnel region is converged inward from an opening to form a horn shape, so that samples gradually flow inward without stopping at the opening, thereby avoiding sample loss.
  • the speed at which blood samples flow to the sampling port in the funnel region is about 1 second, which realizes rapid suction of the blood samples into the sampling port.
  • the notch is provided for fitting the finger pads to facilitate sampling.
  • a further improvement of the present invention is that: the bottom plate layer, the intermediate layer, and the upper cover layer are integrally bonded by means of double-sided gluing of the intermediate layer.
  • the intermediate layer is a pressure-sensitive adhesive tape
  • the material of the upper cover layer and/or the bottom plate layer is any one of PMMA, PP, PE and PET
  • the surfaces of the upper cover layer and the bottom plate layer each has a hydrophilic membrane, so that the samples flow rapidly through the chip sampling port into the main flow channel, and then are distributed to each branch microfluidic channel.
  • the depth and size of the microfluidic channel can be accurately controlled, and it is also convenient to control the depth of the detection chambers, so that the thickness deviation of the detection chambers of the microfluidic chip is small, the consistency is high, and the accuracy of detection is improved.
  • a hydrophilic membrane is disposed on the surfaces of the upper cover layer and the bottom plate layer, so that the samples flow through the chip sampling port into the main flow channel more rapidly, and are distributed to each branch microfluidic channel, which speeds up the flow rate and improves the detection efficiency.
  • the thickness of the intermediate layer is 0.1-1.0 mm
  • the surface of the bottom plate layer is flat
  • the depth of the closed microfluidic channel defined by the bottom plate layer, the intermediate layer, and the upper cover layer that cooperate with each other is 0.1-1.0 mm
  • the width of the detection chambers defined is 1.0-2.0 mm.
  • a nozzle is disposed at the junction of each of the branch microfluidic channels and the corresponding detection chamber, and each of the branch microfluidic channels has a corresponding electrode.
  • Each electrode comprises an input high-side electrode and an input low-side electrode, and the thickness of the electrode is 50 ⁇ m.
  • the electrode is provided for applying a pulse voltage while receiving a signal generated by the blood reaction in the detection chambers.
  • An electrode tip is inserted into a detection instrument, and a detection result is obtained by detecting an electrochemical signal generated by the reaction in cooperation with the supporting detection instrument.
  • the electrode tip is a part of the integrally bonded bottom plate layer, intermediate layer and upper cover layer that is exposed outside relative to the upper cover layer and the intermediate layer, so that the electrode tip can be inserted into the detection instrument more easily and conveniently.
  • the microfluidic detection chip for multi-channel rapid detection is designed with a main flow channel and a plurality of branch microfluidic channels in a specific structural form to guide the flow of blood samples, so that one sample chamber can simultaneously inject samples into a plurality of reaction chambers without contaminating the samples, and it is easy to inject samples.
  • the samples After sampled by the chip sampling port, the samples simultaneously flow through the main flow channel to the plurality of branch microfluidic channels, and then flow into the plurality of independent detection chambers. In this way, the plurality of samples can be simultaneously detected, and the multi-channel effect is achieved.
  • the chip is simple in structure and convenient in operation, thereby improving the detection efficiency and accuracy, greatly reducing the consumption of resources, realizing rapid detection, and lowering the cost.
  • Samples are injected into the chip sampling port 7, and simultaneously flow through the main flow channel 501 to the plurality of branch microfluidic channels 502, and then flow into the plurality of independent detection chambers 8.
  • the samples are reacted with the detection reagents pre-embedded in the detection chambers 8, and the microfluidic detection chip for multi-channel rapid detection is inserted into the detection instrument by means of the electrode tip 401.
  • the detection result is obtained by detecting the electrochemical signal generated by the reaction in cooperation with the supporting detection instrument. In this way, the plurality of samples can be simultaneously detected, and the multi-channel effect is achieved, thereby improving the detection efficiency.

Claims (6)

  1. Mikrofluidischer Detektions-Chip zur Mehrkanal-Schnelldetektion umfassend einen Chipkörper, einen Chipprobeentnahmeport (7), eine Vielzahl unabhängiger Detektionskammern (8), und einen mikrofluidischen Kanal (5), der auf dem Chipkörper angeordnet ist, wobei der Chipprobeentnahmeport (7) mittels des mikrofluidischen Kanals (5) mit den Detektionskammern (8) in Verbindung steht, wobei der Chipkörper weiter eine Elektrode (4) umfasst; die Detektionskammer (8) mit der Elektrode (4) verbunden sind; der mikrofluidische Kanal (5) einen Strömungshauptkanal (501) und eine Vielzahl mikrofluidischer Zweigkanäle (502) umfasst, ein unteres Ende des Strömungshauptkanals (501) in eine Vielzahl mikrofluidischer Zweigkanäle (502) geteilt ist, und die Vielzahl mikrofluidischer Zweigkanäle (502) mit der Vielzahl unabhängiger Detektionskammern (8) in Eins-zu-eins-Korrespondenz in Verbindung steht; und das andere Ende des Strömungshauptkanals (5) mit dem Chipprobeentnahmeport (7) in Verbindung steht, wobei der Chipkörper der Reihe nach von unten nach oben eine Bodenplatteschicht (1), eine Zwischenschicht (2) und eine Oberdeckungsschicht (3) umfasst; die Bodenplatteschicht (1), die Zwischenschicht (2) und die Oberdeckungsschicht (3) zur Bestimmung eines geschlossenen mikrofluidischen Kanals (5) und der Vielzahl unabhängiger Detektionskammern (8) und eines Trichterbereichs (9) zusammenwirken; der mikrofluidische Kanal (5) und die Detektionskammern (8) sich in der Zwischenschicht (2) befinden, ein Flüssigkeitseinspritzungsport (701) und eine Vielzahl von Auslasslöchern (6) auf der Oberdeckungsschicht (3) gebildet sind, die Vielzahl Auslasslöchern (6) auf einer Seite der Oberdeckungsschicht (3), die dem unteren Ende des mikrofluidischen Kanals (5) entspricht, vorgesehen ist, und der Flüssigkeitseinspritzungsport (701) mit einem Vorderende des mikrofluidischen Kanals (5) in Verbindung steht; und die Elektrode (4) auf der Bodenplatteschicht (1) vorgesehen ist, wobei die Vielzahl unabhängiger Detektionskammern (8) fächerförmig verteilt ist, eine Kerbe (10) auf einer Seite des unteren Endes der Bodenplatteschicht (1)gebildet ist; der Flüssigkeitseinspritzungsport (701), der Trichterbereich (9) und die Kerbe (10) jeweils an entsprechenden Positionen auf der Oberdeckungsschicht (3), der Zwischenschicht (2) und der Bodenplatteschicht (1) gebildet sind und unterschiedliche Abmessungen aufweisen; und der Chipprobeentnahmeport (7) durch den Flüssigkeitseinspritzungsport (701), den Trichterbereich (9) und die Kerbe (10) gebildet ist und mit dem Boden der Detektionskammern (8) mittels des mikrofluidischen Kanals (5) verbunden ist, wobei der Chipprobeentnahmeport (7) trichterförmig mit einer großen Bodenplattenoberfläche, einer kleiner Oberdeckungsoberfläche und einer mit einem Trichter versehenen Zwischenschicht ausgebildet ist.
  2. Mikrofluidischer Detektions-Chip zur Mehrkanal-Schnelldetektion nach Anspruch 1, wobei der Flüssigkeitseinspritzungsport (701), der Trichterbereich (9) und die Kerbe (10) alle bogenförmig sind und verschiedene Radianten aufweisen; der Flüssigkeitseinspritzungsport (701) und der Trichterbereich (9) halbkreisbogenförmig sind und der Radius des Trichterbereichs (9) nicht niedriger als der Bogenradius des Flüssigkeitseinspritzungsports (701); ein gebogener Strömungshauptkanal (501) in dem Trichterbereich in eine Vielzahl microfluidischer Zweigkanäle geteilt ist, die in Verbindung mit der Vielzahl unabhängiger Detektionskammern (8) in Eins-zu-eins-Korrespondenz in Verbindung stehen; und die Oberfläche der Kerbe kleiner als die Oberfläche des Trichterbereichs ist;
    oder
    der Strömungshauptkanal ein Trichterbereich (501) ist; der Flüssigkeitseinspritzungsport (701) bogenförmig ist, und sich mit einem Teil des Trichterbereichs (501) überschneidet; der Trichterbereich (501) nach innen zur Bildung einer Hornform konvergiert ist; und der Trichterbereich (9) nach innen in eine Vielzahl mikrofluidischer Zweigkanäle am dessen unteren Ende geteilt ist.
  3. Mikrofluidischer Detektions-Chip zur Mehrkanal-Schnelldetektion nach Anspruch 1, wobei die Bodenplatteschicht (1), die Zwischenschicht (2) und die Oberdeckungsschicht (3) einstückig mittels einer beidseitigen Beleimung der Zwischenschicht geklebt sind.
  4. Mikrofluidischer Detektions-Chip zur Mehrkanal-Schnelldetektion nach einem der Ansprüche 1 bis 3, wobei die Zwischenschicht (2) ein druckempfindliches Klebeband ist; das Material der Oberdeckungsschicht (3) und/oder der Bodenplatteschicht (1) ein der Gruppe aus PMMA, PP, PE und PET ist; und die Oberflächen der Oberdeckungsschicht (3) und der Bodenplatteschicht (1) jede eine hydrophile Membran aufweisen.
  5. Mikrofluidischer Detektions-Chip zur Mehrkanal-Schnelldetektion nach Anspruch 4, wobei die Dicke der Zwischenschicht 0,1 -1,0 mm beträgt; die Oberfläche der Bodenplatteschicht flach ist; die Tiefe des geschlossenen mikrofluidischen Kanals bestimmt durch die Bodenplatteschicht, die Zwischenschicht und die Oberdeckungsschicht, die mit einander zusammenwirken, 0,1 - 1,0 mm beträgt und die Breite der bestimmten Detektionskammern 1,0 - 2,0 mm beträgt.
  6. Mikrofluidischer Detektions-Chip zur Mehrkanal-Schnelldetektion nach Anspruch 4, wobei eine Düse an der Verbindungsstelle jedes der mikrofluidischen Zweigkanäle und der entsprechenden Detektionskammer angeordnet ist, und jede der mikrofluidischen Zweigkanäle eine entsprechende Elektrode aufweist; jede Elektrode eine High-Side-Eingangselektrode und eine Low-Side-Eingangselektrode umfasst, und die Dicke der Elektrode 50 µm beträgt.
EP19819952.3A 2018-06-12 2019-01-24 Mikrofluidischer detektions-chip zur mehrkanal-schnelldetektion Active EP3698872B1 (de)

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CN201810599700.5A CN108745429B (zh) 2018-06-12 2018-06-12 一种多通道快速检测微流体检测芯片
PCT/CN2019/073042 WO2019237742A1 (zh) 2018-06-12 2019-01-24 一种多通道快速检测微流体检测芯片

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EP3698872A1 EP3698872A1 (de) 2020-08-26
EP3698872A4 EP3698872A4 (de) 2020-09-02
EP3698872B1 true EP3698872B1 (de) 2021-10-13

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US (1) US11440006B2 (de)
EP (1) EP3698872B1 (de)
CN (1) CN108745429B (de)
SG (1) SG11202100097VA (de)
WO (1) WO2019237742A1 (de)

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CN108745429B (zh) * 2018-06-12 2023-11-24 南京岚煜生物科技有限公司 一种多通道快速检测微流体检测芯片
CN109709316A (zh) * 2018-12-27 2019-05-03 天津昌和生物医药技术有限公司 一种多靶项微流测试卡及制备方法
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CN108745429A (zh) 2018-11-06
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