EP3551744A1 - Bioréacteur convertible, kit et procédé de conversion de bioréacteur - Google Patents

Bioréacteur convertible, kit et procédé de conversion de bioréacteur

Info

Publication number
EP3551744A1
EP3551744A1 EP17808951.2A EP17808951A EP3551744A1 EP 3551744 A1 EP3551744 A1 EP 3551744A1 EP 17808951 A EP17808951 A EP 17808951A EP 3551744 A1 EP3551744 A1 EP 3551744A1
Authority
EP
European Patent Office
Prior art keywords
bioreactor
kit
reactor
pipe
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP17808951.2A
Other languages
German (de)
English (en)
Inventor
Peter Riegler
Dirk Weuster-Botz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technische Universitaet Muenchen
Original Assignee
Technische Universitaet Muenchen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technische Universitaet Muenchen filed Critical Technische Universitaet Muenchen
Publication of EP3551744A1 publication Critical patent/EP3551744A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C12M25/18—Fixed or packed bed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02—Percolation

Definitions

  • the disclosure relates to a convertible bioreactor, a kit, and a method for converting a bioreactor.
  • a bioreactor is used for biotechnological processes, e.g. synthesis gas fermentation.
  • Synthesis gas fermentation also called syngas fermentation
  • a synthesis gas is used as a carbon and energy source and is converted into fuel and / or chemicals by microorganisms.
  • the microorganisms are arranged in the bioreactor and the synthesis gas is provided to the microorganisms.
  • bioreactors including a stirred-tank reactor, a bubble column reactor, a fixed-bed reactor and a trickling filter reactor.
  • the configuration of the bioreactor has a large influence on the efficiency of the microbiological process for producing the desired compound. However, it is difficult to compare different reactor types.
  • a convertible bioreactor according to claim i, a kit according to claim 3, and a method for converting a bioreactor according to claim 12 are provided. Further embodiments are subject matter of dependent claims.
  • a convertible bioreactor for biotechnological processes e.g. synthesis gas fermentation
  • the bioreactor is configured to be operated in different modes of operation.
  • the mode of operation may depend on components used in the bioreactor.
  • the modes of operation may include an operation as a stirred-tank reactor, a bubble column reactor, a fixed-bed reactor and / or a trickling filter reactor.
  • the bioreactor can be converted to all four reactor configurations. It may be necessary to have a setup and / or sterilization period when switching from one type of operation to another type. During a cultivation of microorganisms, the configuration can be changed directly (without a setup and / or sterilization) from the fixed-bed reactor to the trickling filter configuration.
  • kits for enabling a bioreactor to be operated in different modes of operation comprises a pipe for inserting in the bioreactor.
  • a lower end of the pipe is configured to hold the pipe in the bioreactor.
  • the pipe may be cylindrical.
  • a method for converting a bioreactor for different modes of operation using the kit is disclosed.
  • the bioreactor can be used for synthesis gas fermentation.
  • Microorganisms are provided in the bioreactor, for example in a fluid. Suitable microorganisms are mostly known as acetogens and may include Clostridium Ijungdahlii, Clostridium autoethanogenum, Eurobacterium limosum, Clostridium carboxidivorans P7, Peptostreptococcus productus, and Butyribacterium methylotrophicum, Clostridium aceticum and many more (other suitable microorganisms are disclosed in Groher A, Weuster-Botz D (2016): Comparative reaction engineering analysis of different acetogenic bacteria for gas fermentation. J Biotechnol 228: 82-94).
  • a synthesis gas may be introduced in the bioreactor as carbon and energy source for the microorganisms.
  • the synthesis gas may comprise hydrogen (H 2 ), carbon monoxide (CO) and / or carbon dioxide (C0 2 ).
  • the synthesis gas maybe a mixture of CO:C0 2 in a ratio of 4:1.
  • the bioreactor is adaptable for all kinds of biological processes, including aerobic processes, anaerobic processes, autotrophic processes and heterotrophic processes.
  • the bioreactor may be used for cultivating the microorganisms, in particular for cultivating chemolithoautotrophic microorganisms.
  • the bioreactor maybe sterilized by steam pressure sterilization, e.g. using desalinated water.
  • the bioreactor may comprise a wall surrounding an inner region.
  • a stirring device may be arranged in the inner region of the bioreactor.
  • the stirring device may comprise a stirring shaft.
  • a stirring element may be fixed to the stirring shaft, for example a radial flow impeller (e.g. a Rushton turbine), an axial flow impeller, a radial propeller mixer or an axial propeller mixer.
  • a Rushton turbine is a radial flow impeller which may be used for mixing applications.
  • the Rushton turbine comprises a flat disc with vertical blades which are vertically mounted. The blades may be formed as flat blades, concave blades or semi-circular blades.
  • a coupling unit may be arranged in the bioreactor which is coupled to the stirring shaft and to a drive unit for driving the stirring shaft.
  • the coupling unit may be a magnetic coupling unit.
  • the drive unit may be an electric motor.
  • the bioreactor may further comprise an outlet for sample taking, e.g. a sample taking valve.
  • the outlet may be formed in a bottom of the bioreactor or in the wall of the bioreactor.
  • the bioreactor may comprise means for holding a measurement device and / or a thermal device.
  • one or more openings may be formed in the wall and / or in the bottom of the bioreactor. Several openings may be arranged on the same height of the wall. Alternatively, several openings may be formed at different heights of the wall. In one embodiment, four openings are formed in the wall and four openings are formed in the bottom. Of course, other numbers and arrangements of openings are possible.
  • the measurement device and / or the thermal device may be fixed to the bioreactor by a screw joint.
  • the bioreactor may comprise a fluid outlet and/or a fluid intlet for extracting fluid out of the bioreactor and/or for inserting fluid into the reactor.
  • the fluid outlet and/or the fluid inlet may be configured for being connected to a fluid pumping device for pumping fluid out of and/or into the bioreactor. If fluid is introduced into the bioreactor, the fluid outlet and the fluid inlet respectively allows extracting fluid out of the bioreactor and introducing fluid into the reactor continuously to provide a fixed reaction fluid volume in the bioreactor.
  • the fluid outlet may be formed in the wall and/or in the bottom of the bioreactor and the fluid inlet may be formed in the wall and/or in the top of the bioreactor.
  • the measurement device and / or the thermal device may be inserted through an opening such that at least a portion of the measurement device and / or the thermal device, respectively, is arranged in the inner region.
  • the measurement device and / or the thermal device can be brought in contact with the fluid in the inner region, for example.
  • the measurement device may comprise a pH sensor and / or a temperature sensor.
  • the thermal device may comprise a heating device for heating the inner region and / or a cooling device for cooling the inner region.
  • a heating jacket may be arranged around the bioreactor, which may provide more accurate temperature control.
  • a disc may be provided which may be arranged at the lower end of the pipe. The disc may have a diameter larger than a diameter of the pipe.
  • the disc and the pipe may be two separate components.
  • the disc may have a hole in its center.
  • the hole may be (slightly) smaller than the pipe diameter. In this case, the disc can easily stick to the pipe at any desired height.
  • the disc may be cut radially once, so that the material of the disc can be pulled apart to adjust the height.
  • the disc may hold itself on the pipe, e.g. at a height of approximately 45 mm.
  • the diameter of the disc may be adapted to a diameter of the inner region of the bioreactor such that the disc is insertable in the inner region and fixes the pipe in the bioreactor.
  • the diameter of the disc may be slightly smaller than the diameter of the inner region, e.g. 0.5 mm smaller, 1 mm smaller or 2 mm smaller.
  • the lower end of the pipe may comprise one or more magnets to fix the pipe to the bioreactor.
  • One or more further magnets with opposite magnetic polarization than the one or more magnets may be arranged on the bottom of the bioreactor such that a magnetic connection is formed between the one or more magnets and the one or more further magnets when the pipe is inserted in the bioreactor.
  • One or more holes may be formed in the disc, e.g. as a liquid throughput. Several holes may be arranged in a regular layout, e.g. having same distances between each other, or in an irregular layout.
  • the kit may further comprise a baffle.
  • the baffle may be inserted in the inner region of the bioreactor in order to influence a flow of the fluid in the bioreactor when the fluid is stirred.
  • the baffle may be arranged on an inner surface of the wall.
  • the kit may further comprise an adapter for fixing a measurement device to the bioreactor.
  • the adapter may be configured to fix the measurement device in the means of the bioreactor for holding the measurement device.
  • the adapter may be configured to fix the measurement device in the openings in the wall and / or in the bottom of the bioreactor.
  • the kit may comprise a first adapter which is configured to fix a pH sensor to the bioreactor.
  • the kit may comprise a second adapter which is configured to fix a temperature sensor to the bioreactor.
  • the kit may further comprise a further adapter for fixing a thermal device to the bioreactor.
  • the further adapter may be configured to fix the thermal device in the means of the bioreactor for holding the thermal device.
  • the further adapter may be configured to fix the thermal device in the openings in the wall and / or in the bottom of the bioreactor.
  • the ldt may comprise a third adapter which is configured to fix a heating device to the bioreactor.
  • the ldt may comprise a fourth adapter which is configured to fix a cooling device to the bioreactor.
  • the kit may further comprise a gas feed and a circular sintered metal sparger, e.g. for providing the synthesis gas to the bioreactor.
  • a flexible tube may be provided as gas feed.
  • the tube may be made of silicone rubber.
  • the gas feed may be arranged at the bottom of the bioreactor. In this case, the gas introduced in the bioreactor ascends in the inner region of the bioreactor.
  • the kit maybe circular.
  • the kit may further comprise a fixed-bed material.
  • the fixed-bed material may provide a footing for microorganisms.
  • the fixed-bed material may be a ceramic material, e.g. clay ceramic or glass ceramic (sintered glass).
  • the fixed-bed material may be a porous material, e.g. a porous ceramic. Suitable fixed-bed materials are e.g. Sera Siporax and Fluval BioMax.
  • each microorganism requires its specific fixed-bed material. The compatibility of the microorganism with the fixed-bed material should be
  • the kit may further comprise a triclding filter.
  • the trickling filter may be arranged in an upper region of the bioreactor.
  • the trickling filter may be fixed to the stirring shaft.
  • the trickling filter may comprise a pool for holding a liquid.
  • several trickle openings also called micro openings
  • the trickling filter may further comprise gas openings for a throughput of the synthesis gas.
  • the kit may further comprise a suction unit for the trickle filter configuration in order to guarantee a constant liquid level. The liquid may be pumped out of the bioreactor continuously to provide a fixed reaction volume in the bioreactor.
  • the kit may further comprise a pH control unit configured for selectively controlling the pH of a liquid before the liquid is provided to the trickling filter.
  • the kit may further comprise a temperature control unit configured for selectively controlling the temperature of a liquid before the liquid is provided to the trickling filter.
  • the temperature control unit may for example comprise a water reservoir.
  • the kit may further comprise a fluid circulation unit configured for pumping liquid out of the bioreactor, circulating the fluid through the fluid circulation device, and pumping liquid back into the bioreactor.
  • the fluid circulation unit may be configured for being connected with one or more fluid outtakes and/or with one or more fluid intakes provided in the bioreactor. This allows for the renewal, the capture, the examination, the control and the recirculation of the fluid outside of the reactor.
  • the fluid circulation unit may be functionally connected with a pH control unit to control an amount of liquid circulating between the bioreactor and/or the fluid circulation unit and the pH control unit.
  • the kit may further comprise a metal plate comprising one or more holes in the metal plate.
  • the holes may have a diameter smaller than 20 mm, preferably smaller than 15 mm, more preferably smaller than 10 mm.
  • the metal plate may be configured for being provided inside the bioreactor above a fluid.
  • the metal plate can prevent the expulsion of microorganisms from the fluid, which may occur due to bursting gas bubbles.
  • the metal plate does not prevent or affect the possibility of introducing a fluid into the bioreactor, for example from an upper or a lateral part of the bioreactor, since the fluid can penetrate through the holes provided in the metal plate.
  • the bioreactor may be used to operate as a stirred-tank reactor.
  • the stirring element is fixed to the stirring shaft.
  • the stirring element may be provided as a Rushton turbine.
  • the baffle is arranged on the inner surface of the wall of the bioreactor.
  • a fluid containing the microorganisms is inserted in the bioreactor to be stirred by the stirring element and the synthesis gas is provided.
  • One or more measurement devices and / or one or more thermal devices may be arranged at the bioreactor, either at the wall or at the bottom.
  • a pH sensor may be arranged through an opening in the wall of the bioreactor.
  • a temperature sensor may be inserted through an opening in the bottom of the bioreactor.
  • a heating device and / or a cooling device may be attached to the bioreactor, e.g. through openings in the bottom.
  • a sintered metal sparger may be arranged in a lower portion of the bioreactor.
  • a sample may be taken via a sample taking valve which is arranged at the bottom of the bioreactor.
  • the bioreactor is converted to operate as a bubble column reactor, in particular designed as a gas-lift bioreactor.
  • the stirring element and the baffle are removed from the stirred-tank reactor as described above.
  • the pipe is inserted in the bioreactor such that the pipe surrounds the stirring shaft.
  • the pipe may comprise the disc such that the disc is arranged below the sintered metal sparger when the pipe is inserted.
  • a central region inside the pipe is separated from on outer region outside the pipe.
  • a gas feed is arranged in the bottom of the bioreactor such that e.g. the synthesis gas is driven in the outer region. This causes a stream in the fluid to enable an optimum gas exchange.
  • An axial propeller stirrer can optionally be fixed to the stirring shaft.
  • One or more measurement devices and / or one or more thermal devices may be arranged at the bioreactor, either at the wall or at the bottom.
  • the pH sensor may be fixed through an opening in the wall of the bioreactor, e.g. using the first adapter.
  • the temperature sensor may be inserted through an opening in the bottom of the bioreactor, e.g. using the second adapter. Using the second adapter, the temperature sensor can be arranged such that the sensing element does not extend through the sintered metal sparger.
  • the sintered metal sparger may be circular.
  • the heating device and / or the cooling device may be attached to the bioreactor, e.g. through openings in the wall.
  • the third adapter and the fourth adapter may be used for fixing the heating device and the cooling device, respectively.
  • a heating jacket may be used for more accurate and less invasive temperature control.
  • the bioreactor is converted to operate as a fixed-bed reactor.
  • the pipe from the bubble column (gas-lift) reactor remains in the bioreactor.
  • the outer region between the pipe and the wall of the bioreactor is filled with a fixed-bed material.
  • An axial propeller stirrer can be fixed to the stirring shaft.
  • the microorganisms arrange on the fixed-bed material and immobilize thereon and / or may form a biofilm.
  • a gaseous substrate e.g. synthesis gas
  • a contact of the fixed-bed material with the pH sensor and the temperature sensor should be avoided.
  • the pH sensor and the temperature sensor are advantageously arranged at the bottom of the bioreactor, e.g.
  • the heating device and the cooling device may be arranged through the openings in the wall, e.g. using the third adapter and the fourth adapter, respectively.
  • a heating jacket may be used for more accurate and less im3 ⁇ 4sive temperature control.
  • the bioreactor is converted to operate as a trickling filter reactor. It is advisable that the bioreactor is operated as fixed-bed reactor before changing to the trickling filter reactor operation mode. A change of the mode of operation from the fixed- bed reactor to the trickling filter reactor is possible during operation and while bioprocesses of the microorganisms continue.
  • the fluid can be removed from the bioreactor.
  • a suction pipe may be used. The suction pipe may be introduced through one of the openings in the wall or in the bottom of the bioreactor. Only a small amount of the fluid may be kept at the bottom of the bioreactor at a constant height.
  • the trickling filter is arranged in an upper region of the bioreactor and may be fixed at the stirring shaft.
  • the trickling filter is rotatable which provides an improved liquid distribution across the fixed bed.
  • the trickling filter is at least partially filled with a liquid which is dispensed through trickle openings in the bottom of the trickling filter.
  • the liquid is prepared before it is added to the trickling filter such that the liquid has the proper pH and temperature.
  • the pH sensor and the temperature sensor may remain at the bottom of the bioreactor.
  • a H control unit and a temperature control unit may respectively be used for preparing the liquid before it is added to the trickling filter.
  • the pH control unit may be used continuously.
  • a radial flow impeller can optionally be fixed to the stirring shaft at the bottom of the bioreactor. This allows providing a higher degree of homogenization of the fluid.
  • the axial propeller stirrer may comprise a Rushton turbine.
  • FIG. 1 is a schematic representation of the bioreactor operating as stirred-tank reactor.
  • Fig. 2 is a schematic representation of the bioreactor operating as bubble column (gas-lift) reactor.
  • Fig. 3 is a schematic representation of the bioreactor operating as fixed-bed reactor.
  • Fig. 4 is a schematic representation of the bioreactor operating as trickling filter reactor.
  • like reference numerals refer to like components.
  • Fig. 1 shows a bioreactor operating as stirred-tank reactor.
  • the bioreactor comprises a wall 1 and a bottom 2 which define an inner region.
  • a stirring shaft 3 is arranged which is coupled to a coupling unit 4.
  • the coupling unit 4 may be driven by a drive unit (e.g. an electric motor, not shown) for driving the stirring shaft 3.
  • a Rushton turbine 5 is fixed to the stirring shaft 3.
  • a fluid 6 is filled in the bioreactor. Microorganisms may be inserted separately.
  • a gaseous substrate e.g. synthesis gas
  • a baffle 7 is arranged at an inner surface of the wall 1. The baffle 7 influences the flow of the fluid 6 when the fluid 6 is stirred.
  • a sintered metal sparger 8 is arranged in a lower region of the bioreactor.
  • several openings 9 are provided for receiving a measurement device and / or a thermal device.
  • a pH sensor 10 is arranged in an opening 9 of the wall 1.
  • Another opening 11 is formed in the bottom 2 of the bioreactor.
  • a temperature sensor 12 is introduced in the bioreactor through the other opening 11 such that a sensing element 16 extends through the sintered metal sparger 8.
  • a sample taldng valve 18 is arranged at the bottom 2 of the bioreactor.
  • the stirred-tank reactor may be operated in continuous mode.
  • the bioreactor may have a diameter of 0.125 m and a height of 0.3 m.
  • Four openings 9 may be formed in the wall 1, for example at a height of 0.1 m from the bottom 2.
  • four other openings 11 may be formed, e.g. each having an M20XI.5 screw thread.
  • the bioreactor may be closed by a reactor lid (not shown).
  • the reactor lid may comprise a reflow cooling device, a pressure control device and a pressure control valve.
  • the bioreactor may be operable at a pressure of up to 3.5 bar or of 3.5 bar.
  • the wall 1, the bottom 2 and the reactor lid may be formed of stainless steel.
  • the sintered metal sparger 8 may have a height of 0.036 m in the bioreactor.
  • the sintered metal sparger 8 may provide point source sparging.
  • a stirred-tank reactor similar to the embodiment shown in Fig. 1 is commercially available, e.g. from the company Bioengineering AG. Using the kit disclosed herein, the known reactor may be converted to other reactor types enabling other modes of operation.
  • Fig. 2 shows the bioreactor converted to bubble column (gas-lift) operation.
  • the Rushton turbine 5, the baffle 7, and the gas inlet 13 are removed. Instead, a pipe 19 is arranged around the stirring shaft 3 forming a central region 24 and an outer region 25.
  • a disc 23 with holes is formed at a lower portion of the pipe 19. The disc 23 fixes the pipe 19 in the bioreactor.
  • a gas feed 22 is arranged at the bottom 2 of the bioreactor.
  • a circular sintered metal sparger 17 is arranged above the disc 23. The circular sintered metal sparger 17 may have a height of 0.045 m -
  • a bubble column reactor with an inner circulation is formed by such arrangement of the components.
  • the outer region 25 may also be called “riser” (fumigated volume) and the central region 24 may be called “downcomer” (non-fumigated volume).
  • the circular sintered metal sparger 17 may provide gas over an area of 3*io ⁇ 3 m 2 of the outer region (equals 32 % of the riser).
  • the temperature sensor 12 is held by an adapter 21 at the bottom 2. By this arrangement, the length of the sensing element 16 reaching in the bioreactor is reduced.
  • a heating device 14 and a cooling device 15 are provided in openings 9 of the wall 1.
  • the cooling device 15 is attached to the bioreactor using another adapter 20.
  • the bubble column (gas-lift) reactor may be operated in continuous mode.
  • the pipe 19 may have an outer diameter of 0.0603 m and an inner diameter of 0.0571 m.
  • the pipe 19 may be made from stainless steel.
  • the bubble column (gas-lift) bioreactor may have a fumigated surface (outer region) of 9.4*10-3 m 2 .
  • the inner region may be referred to as non- fumigated surface. It has an area of 2.56*10-3 m 2 . This corresponds to a riser/downcomer relation of 3.68.
  • Fig. 3 shows the bioreactor of fig. 2 converted to a fixed-bed reactor (with inner circulation).
  • An axial propeller stirrer 26 can be fixed to the stirring shaft 3 in order to provide a faster downward circulation.
  • a fixed-bed material 27 is inserted in the outer region 25. The microorganism can immobilize on the fixed-bed material 27.
  • the fixed-bed reactor may be operated in continuous mode.
  • Fig. 4 shows a triclding filter reactor which is a result of a conversion of the fixed-bed reactor shown in Fig. 3.
  • a large part of the fluid 6 is released from the bioreactor after the microorganisms are immobilized to the fixed-bed material 27. Only a small amount of fluid 6 remains at the bottom 2.
  • a trickling filter 28 is fixed at an upper portion of the stirring shaft 3 so that the trickling filter 28 is rotatable.
  • the trickling filter 28 comprises a pool 29 for receiving a liquid. The liquid is dispensed through trickle openings 30 formed in the bottom of the pool 29. Gas openings 31 are also formed in the triclding filter 28 for synthesis gas throughput.

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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
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  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un bioréacteur convertible destiné à des procédés biotechnologiques qui est conçu pour fonctionner dans différents modes de fonctionnement. L'invention concerne en outre un kit permettant de faire fonctionner un bioréacteur dans différents modes de fonctionnement, et un procédé de conversion d'un bioréacteur dans différents modes de fonctionnement à l'aide du kit.
EP17808951.2A 2016-12-08 2017-12-08 Bioréacteur convertible, kit et procédé de conversion de bioréacteur Withdrawn EP3551744A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP16203027.4A EP3333251A1 (fr) 2016-12-08 2016-12-08 Bioréacteur convertible, kit et procédé de conversion d'un bioréacteur
PCT/EP2017/081945 WO2018104495A1 (fr) 2016-12-08 2017-12-08 Bioréacteur convertible, kit et procédé de conversion de bioréacteur

Publications (1)

Publication Number Publication Date
EP3551744A1 true EP3551744A1 (fr) 2019-10-16

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EP16203027.4A Withdrawn EP3333251A1 (fr) 2016-12-08 2016-12-08 Bioréacteur convertible, kit et procédé de conversion d'un bioréacteur
EP17808951.2A Withdrawn EP3551744A1 (fr) 2016-12-08 2017-12-08 Bioréacteur convertible, kit et procédé de conversion de bioréacteur

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EP16203027.4A Withdrawn EP3333251A1 (fr) 2016-12-08 2016-12-08 Bioréacteur convertible, kit et procédé de conversion d'un bioréacteur

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EP (2) EP3333251A1 (fr)
WO (1) WO2018104495A1 (fr)

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