EP3532606A4 - Immunsuppressive mesenchymale zellen und verfahren zur herstellung davon - Google Patents

Immunsuppressive mesenchymale zellen und verfahren zur herstellung davon Download PDF

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Publication number
EP3532606A4
EP3532606A4 EP17866094.0A EP17866094A EP3532606A4 EP 3532606 A4 EP3532606 A4 EP 3532606A4 EP 17866094 A EP17866094 A EP 17866094A EP 3532606 A4 EP3532606 A4 EP 3532606A4
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EP
European Patent Office
Prior art keywords
methods
mesenchymal cells
forming same
immunosuppressive mesenchymal
immunosuppressive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP17866094.0A
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English (en)
French (fr)
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EP3532606A1 (de
Inventor
Holly WOBMA
Mariko Kanai
Gordana Vunjak-Novakovic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Columbia University in the City of New York
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Columbia University in the City of New York
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Publication date
Application filed by Columbia University in the City of New York filed Critical Columbia University in the City of New York
Publication of EP3532606A1 publication Critical patent/EP3532606A1/de
Publication of EP3532606A4 publication Critical patent/EP3532606A4/de
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
    • A61K39/4621—Cellular immunotherapy characterized by the effect or the function of the cells immunosuppressive or immunotolerising
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/46433—Antigens related to auto-immune diseases; Preparations to induce self-tolerance
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/231—Interleukin-10 (IL-10)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/24—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
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    • C12N2523/00—Culture process characterised by temperature

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Biochemistry (AREA)
  • Rheumatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Transplantation (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Pain & Pain Management (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP17866094.0A 2016-10-27 2017-10-27 Immunsuppressive mesenchymale zellen und verfahren zur herstellung davon Withdrawn EP3532606A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662413696P 2016-10-27 2016-10-27
US201762530617P 2017-07-10 2017-07-10
PCT/US2017/058686 WO2018081514A1 (en) 2016-10-27 2017-10-27 Immunosuppressive mesenchymal cells and methods for forming same

Publications (2)

Publication Number Publication Date
EP3532606A1 EP3532606A1 (de) 2019-09-04
EP3532606A4 true EP3532606A4 (de) 2020-07-29

Family

ID=62025493

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EP17866094.0A Withdrawn EP3532606A4 (de) 2016-10-27 2017-10-27 Immunsuppressive mesenchymale zellen und verfahren zur herstellung davon

Country Status (9)

Country Link
US (1) US20190314417A1 (de)
EP (1) EP3532606A4 (de)
JP (1) JP2019534015A (de)
KR (1) KR20190085000A (de)
CN (1) CN110121553A (de)
AU (1) AU2017348306A1 (de)
CA (1) CA3042031A1 (de)
IL (1) IL266251A (de)
WO (1) WO2018081514A1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020086687A1 (en) * 2018-10-23 2020-04-30 The Johns Hopkins University Partioning of adult mesenchymal stem cells
TW202035682A (zh) 2018-12-14 2020-10-01 比利時商普羅米修亞生物科技股份有限公司 表現hla-g之肝先驅細胞及取得包含該等細胞之此等細胞組成物之方法與其用途
JP2022513475A (ja) 2018-12-14 2022-02-08 プロメセラ セラピューティクス エスエイ Ｈｌａ－ｅを発現する肝臓前駆細胞を含む細胞組成物
KR102331880B1 (ko) * 2019-01-29 2021-11-29 연세대학교 산학협력단 엑소좀을 추출하는 방법 및 이에 이용되는 조성물
US20220062347A1 (en) * 2019-03-08 2022-03-03 Niigata University Induction method for macrophage, anti-inflammatory macrophage-inducing agent, and pharmaceutical composition
KR102503349B1 (ko) 2019-05-14 2023-02-23 프로벤션 바이오, 인코포레이티드 제1형 당뇨병을 예방하기 위한 방법 및 조성물
WO2021009778A2 (en) * 2019-07-18 2021-01-21 Pandorum Technologies Private Limited Methods for culturing mesenchymal stem cells, products thereof, and applications thereof
WO2022221672A1 (en) * 2021-04-16 2022-10-20 Ossium Health, Inc. Interferon gamma-primed mesenchymal stromal cells as prophylaxis for graft versus host disease
KR20240054991A (ko) * 2021-08-11 2024-04-26 판도럼 테크놀로지스 프라이뱃 리미티드 중간엽 줄기 세포의 배양 방법, 그의 조성물 및 구현
CN114438022A (zh) * 2021-12-03 2022-05-06 睿仕康(重庆)生物科技有限公司 具有免疫抑制、抗炎功能的间充质干细胞、其制备方法及应用

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WO2014093948A1 (en) * 2012-12-14 2014-06-19 Rutgers, The State University Of New Jersey Methods modulating immunoregulatory effect of stem cells
WO2016053758A1 (en) * 2014-10-01 2016-04-07 Commence Bio, Inc. Induction medium and methods for stem cell culture and therapy

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US8709401B2 (en) * 2011-02-25 2014-04-29 Howmedica Osteonics Corp. Primed stem cells and uses thereof to treat inflammatory conditions in joints
US20160237407A1 (en) * 2015-02-17 2016-08-18 Batu Biologics, Inc. Universal donor chimeric antigen receptor cells

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2014093948A1 (en) * 2012-12-14 2014-06-19 Rutgers, The State University Of New Jersey Methods modulating immunoregulatory effect of stem cells
WO2016053758A1 (en) * 2014-10-01 2016-04-07 Commence Bio, Inc. Induction medium and methods for stem cell culture and therapy

Non-Patent Citations (10)

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Title
BURON F ET AL: "Human Mesenchymal Stem Cells and Immunosuppressive Drug Interactions in Allogeneic Responses: An In Vitro Study Using Human Cells", TRANSPLANTATION PROCEEDINGS, ELSEVIER INC, ORLANDO, FL; US, vol. 41, no. 8, 1 October 2009 (2009-10-01), pages 3347 - 3352, XP026708145, ISSN: 0041-1345, [retrieved on 20091023], DOI: 10.1016/J.TRANSPROCEED.2009.08.030 *
EUN-SOL LEE ET AL: "Adoptive Transfer of Treg Cells Combined with Mesenchymal Stem Cells Facilitates Repopulation of Endogenous Treg Cells in a Murine Acute GVHD Model", PLOS ONE, vol. 10, no. 9, 22 September 2015 (2015-09-22), pages e0138846, XP055700317, DOI: 10.1371/journal.pone.0138846 *
HOLLY M. WOBMA ET AL: "Dual IFN-[gamma]/hypoxia priming enhances immunosuppression of mesenchymal stromal cells through regulatory proteins and metabolic mechanisms", JOURNAL OF IMMUNOLOGY AND REGENERATIVE MEDICINE, vol. 1, 1 March 2018 (2018-03-01), pages 45 - 56, XP055698674, ISSN: 2468-4988, DOI: 10.1016/j.regen.2018.01.001 *
HOLLY MICHELLE WOBMA: "Interferon-gamma/Hypoxia Primed Mesenchymal Stem Cells for an Improved Immunosuppressive Cell Therapy", 1 January 2018 (2018-01-01), XP055698676, Retrieved from the Internet <URL:https://academiccommons.columbia.edu/doi/10.7916/D8ZC9K1D> DOI: 10.7916/D8ZC9K1D *
HONG-CHAO ZHANG ET AL: "Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Stimulated by Hypoxia Promote Angiogenesis Both In Vitro and In Vivo", STEM CELLS AND DEVELOPMENT, vol. 21, no. 18, 10 December 2012 (2012-12-10), pages 3289 - 3297, XP055173734, ISSN: 1547-3287, DOI: 10.1089/scd.2012.0095 *
MOSTAFA EJTEHADIFAR ET AL: "The Effect of Hypoxia on Mesenchymal Stem Cell Biology", ADVANCED PHARMACEUTICAL BULLETIN, vol. 5, no. 2, 1 June 2015 (2015-06-01), pages 141 - 149, XP055698678, ISSN: 2228-5881, DOI: 10.15171/apb.2015.021 *
MYOUNG WOO LEE ET AL: "Strategies to improve the immunosuppressive properties of human mesenchymal stem cells", STEM CELL RESEARCH & THERAPY, BIOMED CENTRAL LTD, LONDON, UK, vol. 6, no. 1, 7 October 2015 (2015-10-07), pages 179, XP021229541, ISSN: 1757-6512, DOI: 10.1186/S13287-015-0178-Y *
NAZMUL HAQUE ET AL: "Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy", THE SCIENTIFIC WORLD JOURNAL, vol. 2013, 1 January 2013 (2013-01-01), pages 1 - 12, XP055567038, DOI: 10.1155/2013/632972 *
See also references of WO2018081514A1 *
YAHAIRA NAALDIJK ET AL: "Migrational changes of mesenchymal stem cells in response to cytokines, growth factors, hypoxia, and aging", EXPERIMENTAL CELL RESEARCH, vol. 338, no. 1, 1 October 2015 (2015-10-01), AMSTERDAM, NL, pages 97 - 104, XP055698677, ISSN: 0014-4827, DOI: 10.1016/j.yexcr.2015.08.019 *

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Publication number Publication date
IL266251A (en) 2019-06-30
AU2017348306A1 (en) 2019-05-23
WO2018081514A1 (en) 2018-05-03
US20190314417A1 (en) 2019-10-17
CN110121553A (zh) 2019-08-13
EP3532606A1 (de) 2019-09-04
JP2019534015A (ja) 2019-11-28
KR20190085000A (ko) 2019-07-17
CA3042031A1 (en) 2018-05-03

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