EP3438279C0 - Verfahren zur messung von proteinen und nukleinsäure mit ultrahoher empfindlichkeit - Google Patents

Verfahren zur messung von proteinen und nukleinsäure mit ultrahoher empfindlichkeit

Info

Publication number
EP3438279C0
EP3438279C0 EP17774110.5A EP17774110A EP3438279C0 EP 3438279 C0 EP3438279 C0 EP 3438279C0 EP 17774110 A EP17774110 A EP 17774110A EP 3438279 C0 EP3438279 C0 EP 3438279C0
Authority
EP
European Patent Office
Prior art keywords
nuclean
ultra
acid
high sensitivity
measuring proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP17774110.5A
Other languages
English (en)
French (fr)
Other versions
EP3438279A1 (de
EP3438279A4 (de
EP3438279B1 (de
Inventor
Toshiaki Miura
Teruki Yoshimura
Satoshi Watabe
Etsuro Ito
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biophenoma Inc
Original Assignee
Biophenoma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biophenoma Inc filed Critical Biophenoma Inc
Publication of EP3438279A1 publication Critical patent/EP3438279A1/de
Publication of EP3438279A4 publication Critical patent/EP3438279A4/de
Application granted granted Critical
Publication of EP3438279C0 publication Critical patent/EP3438279C0/de
Publication of EP3438279B1 publication Critical patent/EP3438279B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/5695—Mycobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/35—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Mycobacteriaceae (F)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90209—Oxidoreductases (1.) acting on NADH or NADPH (1.6), e.g. those with a heme protein as acceptor (1.6.2) (general), Cytochrome-b5 reductase (1.6.2.2) or NADPH-cytochrome P450 reductase (1.6.2.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Plasma & Fusion (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP17774110.5A 2016-03-30 2017-03-07 Verfahren zur messung von proteinen und nukleinsäure mit ultrahoher empfindlichkeit Active EP3438279B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016068686A JP6960210B2 (ja) 2016-03-30 2016-03-30 タンパク質及び核酸の超高感度測定方法
PCT/JP2017/008982 WO2017169565A1 (ja) 2016-03-30 2017-03-07 タンパク質及び核酸の超高感度測定方法

Publications (4)

Publication Number Publication Date
EP3438279A1 EP3438279A1 (de) 2019-02-06
EP3438279A4 EP3438279A4 (de) 2019-11-20
EP3438279C0 true EP3438279C0 (de) 2026-01-07
EP3438279B1 EP3438279B1 (de) 2026-01-07

Family

ID=59964060

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17774110.5A Active EP3438279B1 (de) 2016-03-30 2017-03-07 Verfahren zur messung von proteinen und nukleinsäure mit ultrahoher empfindlichkeit

Country Status (4)

Country Link
US (1) US11702684B2 (de)
EP (1) EP3438279B1 (de)
JP (1) JP6960210B2 (de)
WO (1) WO2017169565A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2657986T3 (es) * 2011-03-23 2018-03-07 Etsuro Ito Método y kit para la medición de muy alta sensibilidad de proteína y ácido nucleico y un novedoso sustrato enzimático
CN114577745A (zh) * 2022-02-28 2022-06-03 深圳市乐土生物医药有限公司 一种检测亚甲基四氢叶酸脱氢酶2活性的方法
JPWO2025004524A1 (de) * 2023-06-30 2025-01-02
WO2025141913A1 (ja) * 2023-12-25 2025-07-03 学校法人早稲田大学 微量物質の分析方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3034969B2 (ja) * 1991-03-01 2000-04-17 旭化成工業株式会社 アンモニア、α−アミノ酸類またはα−ケト酸の高感度定量法および高感度定量用組成物
EP0632133B1 (de) * 1991-12-12 1998-04-22 Asahi Kasei Kogyo Kabushiki Kaisha Hochempfindliche bestimmung von d-3-hydroxybutiersäure oder acetessigsäure und zusammensetzung dafür
JP3981190B2 (ja) * 1997-07-07 2007-09-26 シスメックス株式会社 コレステロールの定量方法及び定量用試薬
JP3869603B2 (ja) * 1999-12-03 2007-01-17 株式会社トクヤマ ホモシステインの定量方法並びにその試薬
JP5044786B2 (ja) * 2007-02-06 2012-10-10 大学共同利用機関法人自然科学研究機構 ２−デオキシグルコースの代謝速度の測定方法
JP5500985B2 (ja) * 2007-03-28 2014-05-21 悦朗 伊藤 タンパク質および核酸の超高感度測定法
ES2657986T3 (es) 2011-03-23 2018-03-07 Etsuro Ito Método y kit para la medición de muy alta sensibilidad de proteína y ácido nucleico y un novedoso sustrato enzimático

Also Published As

Publication number Publication date
EP3438279A1 (de) 2019-02-06
EP3438279A4 (de) 2019-11-20
US20190106727A1 (en) 2019-04-11
WO2017169565A1 (ja) 2017-10-05
JP6960210B2 (ja) 2021-11-05
US11702684B2 (en) 2023-07-18
JP2017176039A (ja) 2017-10-05
EP3438279B1 (de) 2026-01-07

Similar Documents

Publication Publication Date Title
EP4039178C0 (de) Messvorrichtung und verfahren zur messung von änderungen in der chromophorkonzentration
EP3469984C0 (de) Vorrichtung und verfahren zur messung von bioinformationen
EP3576626A4 (de) System und verfahren zur messung von wahrnehmungserfahrungen
EP3416540C0 (de) Vorrichtung zur messung der konzentration von zielsubstanzen im blut
EP3487407C0 (de) Verfahren und systeme zur messung von analyten mithilfe von kalibrierbaren teststreifen
EP3508124A4 (de) Vorrichtung zur biologischen messung und verfahren zur biologischen messung
EP3345541C0 (de) Vorrichtung und verfahren zur messung von vitalzeichen
EP3518753A4 (de) Systeme und verfahren zur messung biologischer metriken
EP3552137A4 (de) System und verfahren zur erkennung von cryptoware
EP3554681A4 (de) System und verfahren für protein-corona-sensoranordnung zur frühzeitigen erkennung von krankheiten
DE112016005622A5 (de) Magnetischer Umdrehungszähler und Verfahren zur Bestimmung von mit diesem Umdrehungszähler ermittelbaren Umdrehungszahlen
EP3661255A4 (de) Verfahren zur messung der signalstärke, zugehörige vorrichtung und system
EP3479058C0 (de) Verfahren und vorrichtung zur vermessung von karosserieschäden
EP3485271C0 (de) Verfahren und systeme zur messung und inspektion von rohrförmigen waren
EP3542131C0 (de) System und verfahren zur selbstkalibrierenden trägheitsmessung
EP3369382C0 (de) Verfahren und vorrichtung zur erkennung von elastizität
EP3210008C0 (de) Verfahren und vorrichtung zur erkennung von substanzen auf oberflächen
EP3726754A4 (de) Verfahren und vorrichtung zur bestimmung von wegverlusten
EP3458606C0 (de) Verfahren zur identifizierung von proben
EP3755226C0 (de) System und verfahren zur erkennung und messung affektiver zustände
EP3654234C0 (de) System und verfahren zur erkennung von bewegten objekten
EP3683579C0 (de) Verfahren zur detektion von wirtszellenproteinen
EP3492600C0 (de) Verfahren zur messung von glykoliertem hämoglobin
EP3356810C0 (de) Fluidanalysator zur messung von magnesiumionen und verfahren zu dessen kalibrierung
EP3562385A4 (de) Verfahren und system zur erfassung von bildern zur wundbeurteilung mit feuchtigkeitserkennung

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20181024

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20191017

RIC1 Information provided on ipc code assigned before grant

Ipc: G01N 33/535 20060101ALI20191011BHEP

Ipc: G01N 33/53 20060101ALI20191011BHEP

Ipc: C12Q 1/68 20180101ALI20191011BHEP

Ipc: C12Q 1/32 20060101AFI20191011BHEP

REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40007413

Country of ref document: HK

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

17Q First examination report despatched

Effective date: 20201211

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: ITO, ETSURO

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: BIOPHENOMA, INC.

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

INTG Intention to grant announced

Effective date: 20250908

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

RAP3 Party data changed (applicant data changed or rights of an application transferred)

Owner name: BIOPHENOMA, INC.

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: CH

Ref legal event code: F10

Free format text: ST27 STATUS EVENT CODE: U-0-0-F10-F00 (AS PROVIDED BY THE NATIONAL OFFICE)

Effective date: 20260107

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602017093454

Country of ref document: DE

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

U01 Request for unitary effect filed

Effective date: 20260130

U07 Unitary effect registered

Designated state(s): AT BE BG DE DK EE FI FR IT LT LU LV MT NL PT RO SE SI

Effective date: 20260204

U20 Renewal fee for the european patent with unitary effect paid

Year of fee payment: 10

Effective date: 20260218