EP3375523A1 - Mikrofluidische vorrichtung - Google Patents

Mikrofluidische vorrichtung Download PDF

Info

Publication number
EP3375523A1
EP3375523A1 EP18161894.3A EP18161894A EP3375523A1 EP 3375523 A1 EP3375523 A1 EP 3375523A1 EP 18161894 A EP18161894 A EP 18161894A EP 3375523 A1 EP3375523 A1 EP 3375523A1
Authority
EP
European Patent Office
Prior art keywords
microfluidic
micro
fluidic
card
component
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP18161894.3A
Other languages
English (en)
French (fr)
Other versions
EP3375523B1 (de
Inventor
François BOIZOT
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat a lEnergie Atomique CEA, Commissariat a lEnergie Atomique et aux Energies Alternatives CEA filed Critical Commissariat a lEnergie Atomique CEA
Publication of EP3375523A1 publication Critical patent/EP3375523A1/de
Application granted granted Critical
Publication of EP3375523B1 publication Critical patent/EP3375523B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions

Definitions

  • the present invention relates to a microfluidic device.
  • This device comprises in particular a micro-fluidic card on which is assembled a microfluidic component using assembly means.
  • Microfluidic devices are well known and are particularly used in the field of research in biology, medicine or pharmacy. They make it possible to carry out fluidic interconnections between several liquid or gaseous compounds. They can be in various forms, including in the form of a card having dimensions similar to those of a credit card.
  • the card thus comprises a microfluidic structure adapted to its application which may comprise different microfluidic elements. These microfluidic elements are, for example, micro-fluidic input and output connection points, microfluidic channels, chambers, micro-valves, etc.
  • the card is for example positioned in a suitable housing of the invention. an automaton which controls various operations, in particular the injection of the different fluids through the input connection points and their evacuation via the output connection points of the microfluidic structure of the card.
  • the microfluidic structure of the card In order to standardize the card and its microfluidic structure with respect to several applications, the microfluidic structure of the card must be easily modified and adapted to the intended application. This adaptation must be carried out easily and without risk of malfunction, in particular by respecting the tightness of the device.
  • the object of the invention is to provide a microfluidic device whose microfluidic structure can be easily modified and thus adapted to a particular application.
  • microfluidic device as defined in claim 1.
  • the micro-fluidic card 2 is for example made of a plastic material such as PMMA (polymethyl methacrylate), COC ("Cyclic Olefin Copolymer” - cycloolefin polymer) or other similar material.
  • PMMA polymethyl methacrylate
  • COC Cyclic Olefin Copolymer
  • Micro-fluidic elements may in particular be formed on two depth levels of the card, being made separately in the first layer 20 and in the second layer 21 of the card.
  • impressions are for example made by machining the surface of the corresponding layer.
  • microfluidic card 2 manufacture of such a microfluidic card 2 is well known, it is not detailed in the present application.
  • the card 2 thus formed comprises a so-called upper face 200, a so-called lower face 210 and a wafer 220 forming its thickness.
  • the microfluidic elements are formed in the thickness of the card 2 and accessible by micro-fluidic connection points 201 of inlet and outlet which open and which are accessible on the upper face 200 and / or on the lower face 210 of the map 2.
  • the card also comprises microfluidic channels 202 connecting different points of micro-fluidic connection 201 so as to form an interconnected network of micro-fluidic elements.
  • the card 2 On one of its faces, for example the upper face 200 (as represented on the Figures 1 and 2 ), the card 2 comprises at least one bearing surface, said first bearing surface forming a specific location for receiving a removable microfluidic component 3 as described below.
  • the microfluidic device 1 comprises in fact a microfluidic component 3 intended to be assembled on the card 2.
  • This component 3 comprises for example two opposite main faces, said upper face and lower face, separated from each other by the thickness of the component, forming its edge.
  • This component has for example a parallelepipedic general shape. It also comprises on its underside a surface, said second bearing surface, intended to come opposite the first bearing surface formed on the card 2 and in a plane parallel to this first bearing surface. .
  • This microfluidic component 3 also comprises a microfluidic structure, called the second microfluidic structure, composed of microfluidic elements such as those exemplified above.
  • the microfluidic component 3 comprises microfluidic connection points 311 and microfluidic channels 312 connecting the microfluidic connection points 311 so as to form an interconnected network of microfluidic elements.
  • This microfluidic component 3 is removable relative to the card 2.
  • the second microfluidic structure provided by the component 3 is connected to the first microfluidic structure of the card 2 via connection points available on the board and connected to the micro-fluidic structure of the board.
  • micro-fluidic connection points 201 of the card are connected to microfluidic connection points 311 of the component for interconnecting the two microfluidic structures.
  • the component 3 is removable, it can be replaced by a component 3 having the same microfluidic structure or chosen with a specific microfluidic structure depending on the application for which the microfluidic device 1 is intended. the invention.
  • the microfluidic component 3 of the device of the invention is in two parts. It comprises a first part 30 which carries assembly means of the component 3 on the card 2 and a second part 31 which comprises the micro-fluidic structure of the component.
  • the two parts 30, 31 can be made of identical or different materials.
  • the first part is arranged to keep the second part in position on the map.
  • the two-part architecture of the component 3 advantageously enables it to separate its first part 30 dedicated to the mechanical fixing of its second part 31 dedicated to the microfluidic application. It is thus possible to keep the first part 30 of the component 3 while modifying its microfluidic structure, simply by replacing its second part 31 with another having an identical or different microfluidic structure.
  • the first part 30 of the microfluidic component is for example in the form of a rectangular frame having an upper surface and a lower surface.
  • the second portion 31 of the microfluidic component 3 is for example in the form of a micro-fluidic plate or chip which is positioned in abutment against the lower surface of the frame formed by the first part 30 so as to be held by the latter.
  • This microfluidic chip is for example made of a material such as silicon. Its microfluidic structure is created by known processes.
  • the microfluidic device 1 of the invention comprises at least one advantageously flat gasket 4 intended to be positioned between the first bearing surface and the second bearing surface described above, so as to realize between the card 2 and the component 3.
  • this seal is sandwiched between the plate or chip formed by the second part 31 of the micro-component. fluidic 3 and the upper face 200 of the microfluidic card 2.
  • This seal consists for example of a strip or a plate, made of flexible material such as for example rubber or silicone.
  • the silicone seal has the particularity of adhering very well to a silicon surface such as that which composes the chip of the second portion 31 of the microfluidic component 3.
  • such a silicone seal is repositionable at will. on the silicon chip.
  • the seal 4 comprises several orifices 40 made through the thickness of the strip and each intended to be positioned in the axis of a microfluidic connection point 201 of the card and a micro connection point. -fluidic 311 of the component so as to seal the connection between the two points and therefore, more generally, between the micro-fluidic structure of the card 2 and the microfluidic structure of the component 3 when the two structures are interconnected.
  • the device 1 comprises for example two seals of this type for each microfluidic component 3 in order to seal micro-fluidic input connection points and micro-fluidic connection points. Release. These may be identical or different, in terms of dimensions or in the position of the orifices ...
  • each seal is positioned in a suitable manner to ensure the tight junction of several connection points at the same time using a single seal.
  • Each seal is advantageously fixed on the upper face 200 of the microfluidic card 2 or on the face facing the microfluidic component 3.
  • the seal is flat and comprises, on one of its two flat faces, a bead 41 or extra thickness made in the direction of the thickness of the seal around each orifice 40 and against which comes apply compression.
  • the seal 4 can be made integral with the microfluidic card 2 or the microfluidic component 3, before mounting the component 3 on the card 2.
  • the device 1 comprises means for centering and assembling the microfluidic component 3 on the micro-fluidic card 2. These centering and assembling means are arranged to ensure sufficient compression of the seal 4. These means comprise at least one magnetic device.
  • the centering and assembly means also comprise a mechanical device cooperating with said magnetic device to ensure the centering and assembly of the component on the card.
  • the magnetic device thus integrates at least two magnetic effect assembly elements arranged to attract by magnetic effect.
  • the mechanical device preferably comprises a male / female type assembly solution. It thus comprises, for example, studs 303 and corresponding cavities 203.
  • said pads 303 are for example made on the microfluidic component 3 while the cavities are formed on the micro-fluidic card 2.
  • Said cavities 203 are arranged on the upper face 200 of the card, around the first bearing surface, and are each intended to receive a corresponding pad 303 made on the component 3.
  • the pads 303 are arranged to position and maintain the second part of the component in position on the card 2
  • the studs 303 are advantageously formed on the first part 30 of the component 3 and are distributed on the second bearing surface of the microfluidic component 3.
  • the second bearing surface is made wholly or partly on the second part 31 of the component.
  • the second part 31 of the microfluidic component 3 comprises the microfluidic structure of the component 3, that is to say in particular the micro-fluidic connection points 311 of inlet and / or outlet and the microfluidic channels. 312 mentioned above.
  • these microfluidic connection points 311 are intended to connect to micro-fluidic connection points 201 of input and / or output made on the card 2 to connect the microfluidic structure of the component 3 to the micro-fluidic structure of the card 2.
  • the pads 303 are for example in the form of a cylinder of revolution and the cavities 203 have a corresponding female shape.
  • the magnetic device comprises several magnetic assembly elements such as permanent magnets 50, each forming, by their shape, a stud 303 of the mechanical device or housed in a stud 303 of the mechanical device to associate the stud with a magnetic effect.
  • the magnetic device also comprises several other magnetic-effect assembly elements, for example also permanent magnets 60, which are advantageously housed in the thickness of the card, so as to have a face in the bottom of each cavity 203 or separate from the bottom of said cavity 203 by a map thickness at this location.
  • These permanent magnets 60 are for example housed in spaces formed in the second layer 21 of the micro-fluidic card 2 while the cavities 203 are formed by orifices that pass through or not the first layer 20 of the card. According to this particular embodiment, the permanent magnets 50 are positioned at the four corners of the component. Each cavity 203 can indeed be non-through through the first layer 20. Thus, each permanent magnet 60 is trapped in the second layer 21 of the card 2 once the two layers 20, 21 are assembled one on the other. 'other.
  • an air gap (referenced e on the figure 3B ) is advantageously left between each permanent magnet 50 of the component and the corresponding permanent magnet 60 of the micro-fluidic card 2 (or, more generally, between two magnetic-effect assembly elements).
  • each cavity 203 with a depth greater than the height of each corresponding stud 303 of the component.
  • the presence of air gaps has the advantage of ensuring uniform compression of the seal 4 by the magnetic effect.
  • the gap can be made at least by the thickness of material present between the bottom of the cavity 203 made in the first layer 20 and the magnet 60 present below in the second layer 21.
  • the bead 41 made on the surface of the seal around each of its orifices 40 ensures the exercise of a maximum force on the surface of the bead 41 by the magnetic effect.
  • micro-fluidic card 2 is shown with two separate locations to accommodate two components of the type described above on the same card.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micromachines (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
EP18161894.3A 2017-03-17 2018-03-15 Mikrofluidische vorrichtung Active EP3375523B1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1752178A FR3063990A1 (fr) 2017-03-17 2017-03-17 Dispositif micro-fluidique

Publications (2)

Publication Number Publication Date
EP3375523A1 true EP3375523A1 (de) 2018-09-19
EP3375523B1 EP3375523B1 (de) 2021-01-06

Family

ID=58993039

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18161894.3A Active EP3375523B1 (de) 2017-03-17 2018-03-15 Mikrofluidische vorrichtung

Country Status (2)

Country Link
EP (1) EP3375523B1 (de)
FR (1) FR3063990A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024182388A1 (en) * 2023-02-27 2024-09-06 Arizona Board Of Regents On Behalf Of Arizona State University Apparatus containing multiple microfluidic assay devices with scaffold regions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100322826A1 (en) * 2009-06-23 2010-12-23 National Institute Of Standards And Technology Magnetic connectors for microfluidic applications
US20120085644A1 (en) * 2010-10-07 2012-04-12 Renzi Ronald F Fluid delivery manifolds and microfluidic systems
WO2015175188A1 (en) * 2014-05-14 2015-11-19 General Electric Company Microfluidic flow cell assemblies for imaging and method of use

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3415611A1 (de) * 2016-01-07 2018-12-19 Vanderbilt University Verbindungen mehrerer manipulierter perfundierter gewebekonstrukte und mikrobioreaktoren, multi-mikroformulator und anwendungen davon

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100322826A1 (en) * 2009-06-23 2010-12-23 National Institute Of Standards And Technology Magnetic connectors for microfluidic applications
US20120085644A1 (en) * 2010-10-07 2012-04-12 Renzi Ronald F Fluid delivery manifolds and microfluidic systems
WO2015175188A1 (en) * 2014-05-14 2015-11-19 General Electric Company Microfluidic flow cell assemblies for imaging and method of use

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024182388A1 (en) * 2023-02-27 2024-09-06 Arizona Board Of Regents On Behalf Of Arizona State University Apparatus containing multiple microfluidic assay devices with scaffold regions

Also Published As

Publication number Publication date
EP3375523B1 (de) 2021-01-06
FR3063990A1 (fr) 2018-09-21

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