EP3375523A1 - Microfluidic device - Google Patents

Microfluidic device Download PDF

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Publication number
EP3375523A1
EP3375523A1 EP18161894.3A EP18161894A EP3375523A1 EP 3375523 A1 EP3375523 A1 EP 3375523A1 EP 18161894 A EP18161894 A EP 18161894A EP 3375523 A1 EP3375523 A1 EP 3375523A1
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EP
European Patent Office
Prior art keywords
microfluidic
micro
fluidic
card
component
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP18161894.3A
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German (de)
French (fr)
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EP3375523B1 (en
Inventor
François BOIZOT
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions

Definitions

  • the present invention relates to a microfluidic device.
  • This device comprises in particular a micro-fluidic card on which is assembled a microfluidic component using assembly means.
  • Microfluidic devices are well known and are particularly used in the field of research in biology, medicine or pharmacy. They make it possible to carry out fluidic interconnections between several liquid or gaseous compounds. They can be in various forms, including in the form of a card having dimensions similar to those of a credit card.
  • the card thus comprises a microfluidic structure adapted to its application which may comprise different microfluidic elements. These microfluidic elements are, for example, micro-fluidic input and output connection points, microfluidic channels, chambers, micro-valves, etc.
  • the card is for example positioned in a suitable housing of the invention. an automaton which controls various operations, in particular the injection of the different fluids through the input connection points and their evacuation via the output connection points of the microfluidic structure of the card.
  • the microfluidic structure of the card In order to standardize the card and its microfluidic structure with respect to several applications, the microfluidic structure of the card must be easily modified and adapted to the intended application. This adaptation must be carried out easily and without risk of malfunction, in particular by respecting the tightness of the device.
  • the object of the invention is to provide a microfluidic device whose microfluidic structure can be easily modified and thus adapted to a particular application.
  • microfluidic device as defined in claim 1.
  • the micro-fluidic card 2 is for example made of a plastic material such as PMMA (polymethyl methacrylate), COC ("Cyclic Olefin Copolymer” - cycloolefin polymer) or other similar material.
  • PMMA polymethyl methacrylate
  • COC Cyclic Olefin Copolymer
  • Micro-fluidic elements may in particular be formed on two depth levels of the card, being made separately in the first layer 20 and in the second layer 21 of the card.
  • impressions are for example made by machining the surface of the corresponding layer.
  • microfluidic card 2 manufacture of such a microfluidic card 2 is well known, it is not detailed in the present application.
  • the card 2 thus formed comprises a so-called upper face 200, a so-called lower face 210 and a wafer 220 forming its thickness.
  • the microfluidic elements are formed in the thickness of the card 2 and accessible by micro-fluidic connection points 201 of inlet and outlet which open and which are accessible on the upper face 200 and / or on the lower face 210 of the map 2.
  • the card also comprises microfluidic channels 202 connecting different points of micro-fluidic connection 201 so as to form an interconnected network of micro-fluidic elements.
  • the card 2 On one of its faces, for example the upper face 200 (as represented on the Figures 1 and 2 ), the card 2 comprises at least one bearing surface, said first bearing surface forming a specific location for receiving a removable microfluidic component 3 as described below.
  • the microfluidic device 1 comprises in fact a microfluidic component 3 intended to be assembled on the card 2.
  • This component 3 comprises for example two opposite main faces, said upper face and lower face, separated from each other by the thickness of the component, forming its edge.
  • This component has for example a parallelepipedic general shape. It also comprises on its underside a surface, said second bearing surface, intended to come opposite the first bearing surface formed on the card 2 and in a plane parallel to this first bearing surface. .
  • This microfluidic component 3 also comprises a microfluidic structure, called the second microfluidic structure, composed of microfluidic elements such as those exemplified above.
  • the microfluidic component 3 comprises microfluidic connection points 311 and microfluidic channels 312 connecting the microfluidic connection points 311 so as to form an interconnected network of microfluidic elements.
  • This microfluidic component 3 is removable relative to the card 2.
  • the second microfluidic structure provided by the component 3 is connected to the first microfluidic structure of the card 2 via connection points available on the board and connected to the micro-fluidic structure of the board.
  • micro-fluidic connection points 201 of the card are connected to microfluidic connection points 311 of the component for interconnecting the two microfluidic structures.
  • the component 3 is removable, it can be replaced by a component 3 having the same microfluidic structure or chosen with a specific microfluidic structure depending on the application for which the microfluidic device 1 is intended. the invention.
  • the microfluidic component 3 of the device of the invention is in two parts. It comprises a first part 30 which carries assembly means of the component 3 on the card 2 and a second part 31 which comprises the micro-fluidic structure of the component.
  • the two parts 30, 31 can be made of identical or different materials.
  • the first part is arranged to keep the second part in position on the map.
  • the two-part architecture of the component 3 advantageously enables it to separate its first part 30 dedicated to the mechanical fixing of its second part 31 dedicated to the microfluidic application. It is thus possible to keep the first part 30 of the component 3 while modifying its microfluidic structure, simply by replacing its second part 31 with another having an identical or different microfluidic structure.
  • the first part 30 of the microfluidic component is for example in the form of a rectangular frame having an upper surface and a lower surface.
  • the second portion 31 of the microfluidic component 3 is for example in the form of a micro-fluidic plate or chip which is positioned in abutment against the lower surface of the frame formed by the first part 30 so as to be held by the latter.
  • This microfluidic chip is for example made of a material such as silicon. Its microfluidic structure is created by known processes.
  • the microfluidic device 1 of the invention comprises at least one advantageously flat gasket 4 intended to be positioned between the first bearing surface and the second bearing surface described above, so as to realize between the card 2 and the component 3.
  • this seal is sandwiched between the plate or chip formed by the second part 31 of the micro-component. fluidic 3 and the upper face 200 of the microfluidic card 2.
  • This seal consists for example of a strip or a plate, made of flexible material such as for example rubber or silicone.
  • the silicone seal has the particularity of adhering very well to a silicon surface such as that which composes the chip of the second portion 31 of the microfluidic component 3.
  • such a silicone seal is repositionable at will. on the silicon chip.
  • the seal 4 comprises several orifices 40 made through the thickness of the strip and each intended to be positioned in the axis of a microfluidic connection point 201 of the card and a micro connection point. -fluidic 311 of the component so as to seal the connection between the two points and therefore, more generally, between the micro-fluidic structure of the card 2 and the microfluidic structure of the component 3 when the two structures are interconnected.
  • the device 1 comprises for example two seals of this type for each microfluidic component 3 in order to seal micro-fluidic input connection points and micro-fluidic connection points. Release. These may be identical or different, in terms of dimensions or in the position of the orifices ...
  • each seal is positioned in a suitable manner to ensure the tight junction of several connection points at the same time using a single seal.
  • Each seal is advantageously fixed on the upper face 200 of the microfluidic card 2 or on the face facing the microfluidic component 3.
  • the seal is flat and comprises, on one of its two flat faces, a bead 41 or extra thickness made in the direction of the thickness of the seal around each orifice 40 and against which comes apply compression.
  • the seal 4 can be made integral with the microfluidic card 2 or the microfluidic component 3, before mounting the component 3 on the card 2.
  • the device 1 comprises means for centering and assembling the microfluidic component 3 on the micro-fluidic card 2. These centering and assembling means are arranged to ensure sufficient compression of the seal 4. These means comprise at least one magnetic device.
  • the centering and assembly means also comprise a mechanical device cooperating with said magnetic device to ensure the centering and assembly of the component on the card.
  • the magnetic device thus integrates at least two magnetic effect assembly elements arranged to attract by magnetic effect.
  • the mechanical device preferably comprises a male / female type assembly solution. It thus comprises, for example, studs 303 and corresponding cavities 203.
  • said pads 303 are for example made on the microfluidic component 3 while the cavities are formed on the micro-fluidic card 2.
  • Said cavities 203 are arranged on the upper face 200 of the card, around the first bearing surface, and are each intended to receive a corresponding pad 303 made on the component 3.
  • the pads 303 are arranged to position and maintain the second part of the component in position on the card 2
  • the studs 303 are advantageously formed on the first part 30 of the component 3 and are distributed on the second bearing surface of the microfluidic component 3.
  • the second bearing surface is made wholly or partly on the second part 31 of the component.
  • the second part 31 of the microfluidic component 3 comprises the microfluidic structure of the component 3, that is to say in particular the micro-fluidic connection points 311 of inlet and / or outlet and the microfluidic channels. 312 mentioned above.
  • these microfluidic connection points 311 are intended to connect to micro-fluidic connection points 201 of input and / or output made on the card 2 to connect the microfluidic structure of the component 3 to the micro-fluidic structure of the card 2.
  • the pads 303 are for example in the form of a cylinder of revolution and the cavities 203 have a corresponding female shape.
  • the magnetic device comprises several magnetic assembly elements such as permanent magnets 50, each forming, by their shape, a stud 303 of the mechanical device or housed in a stud 303 of the mechanical device to associate the stud with a magnetic effect.
  • the magnetic device also comprises several other magnetic-effect assembly elements, for example also permanent magnets 60, which are advantageously housed in the thickness of the card, so as to have a face in the bottom of each cavity 203 or separate from the bottom of said cavity 203 by a map thickness at this location.
  • These permanent magnets 60 are for example housed in spaces formed in the second layer 21 of the micro-fluidic card 2 while the cavities 203 are formed by orifices that pass through or not the first layer 20 of the card. According to this particular embodiment, the permanent magnets 50 are positioned at the four corners of the component. Each cavity 203 can indeed be non-through through the first layer 20. Thus, each permanent magnet 60 is trapped in the second layer 21 of the card 2 once the two layers 20, 21 are assembled one on the other. 'other.
  • an air gap (referenced e on the figure 3B ) is advantageously left between each permanent magnet 50 of the component and the corresponding permanent magnet 60 of the micro-fluidic card 2 (or, more generally, between two magnetic-effect assembly elements).
  • each cavity 203 with a depth greater than the height of each corresponding stud 303 of the component.
  • the presence of air gaps has the advantage of ensuring uniform compression of the seal 4 by the magnetic effect.
  • the gap can be made at least by the thickness of material present between the bottom of the cavity 203 made in the first layer 20 and the magnet 60 present below in the second layer 21.
  • the bead 41 made on the surface of the seal around each of its orifices 40 ensures the exercise of a maximum force on the surface of the bead 41 by the magnetic effect.
  • micro-fluidic card 2 is shown with two separate locations to accommodate two components of the type described above on the same card.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micromachines (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

L'invention concerne un dispositif micro-fluidique qui comporte une carte micro-fluidique (2) présentant une première structure micro-fluidique et un composant micro-fluidique (3) qui comporte une deuxième micro-fluidique destinée à être interconnectée avec la première structure micro-fluidique. Le dispositif comporte notamment des moyens d'assemblage et de centrage du composant micro-fluidique (3) sur la carte (2) et comprenant au moins un dispositif magnétique. Un joint d'étanchéité (4) est positionné entre ladite carte micro-fluidique (2) et ledit composant micro-fluidique et est agencé pour assurer une connexion étanche entre la première structure micro-fluidique de la carte micro-fluidique (2) et la deuxième structure micro-fluidique du composant micro-fluidique (3).

Figure imgaf001
The invention relates to a microfluidic device which comprises a micro-fluidic card (2) having a first microfluidic structure and a microfluidic component (3) which comprises a second microfluidic intended to be interconnected with the first structure. microfluidics. The device comprises in particular means for assembling and centering the microfluidic component (3) on the card (2) and comprising at least one magnetic device. A seal (4) is positioned between said microfluidic board (2) and said microfluidic component and is arranged to provide a tight connection between the first microfluidic structure of the micro-fluidic board (2) and the second microfluidic structure of the microfluidic component (3).
Figure imgaf001

Description

Domaine technique de l'inventionTechnical field of the invention

La présente invention se rapporte à un dispositif micro-fluidique. Ce dispositif comporte notamment une carte micro-fluidique sur laquelle est assemblé un composant micro-fluidique à l'aide de moyens d'assemblage.The present invention relates to a microfluidic device. This device comprises in particular a micro-fluidic card on which is assembled a microfluidic component using assembly means.

Etat de la techniqueState of the art

Les dispositifs micro-fluidiques sont bien connus et sont notamment employés dans le domaine de la recherche en biologie, en médecine ou en pharmacie. Ils permettent de réaliser des interconnexions fluidiques entre plusieurs composés liquides ou gazeux. Ils peuvent se présenter sous différentes formes, notamment sous la forme d'une carte ayant des dimensions similaires à celles d'une carte de crédit. La carte comporte ainsi une structure micro-fluidique adaptée à son application qui peut comporter différents éléments micro-fluidiques. Ces éléments micro-fluidiques sont par exemple des points de connexion micro-fluidique d'entrée et de sortie, des canaux micro-fluidiques, des chambres, des micro-vannes,... La carte est par exemple positionnée dans un logement adapté d'un automate qui commande différentes opérations, notamment l'injection des différents fluides à travers les points de connexion d'entrée et leur évacuation par les points de connexion de sortie de la structure micro-fluidique de la carte.Microfluidic devices are well known and are particularly used in the field of research in biology, medicine or pharmacy. They make it possible to carry out fluidic interconnections between several liquid or gaseous compounds. They can be in various forms, including in the form of a card having dimensions similar to those of a credit card. The card thus comprises a microfluidic structure adapted to its application which may comprise different microfluidic elements. These microfluidic elements are, for example, micro-fluidic input and output connection points, microfluidic channels, chambers, micro-valves, etc. The card is for example positioned in a suitable housing of the invention. an automaton which controls various operations, in particular the injection of the different fluids through the input connection points and their evacuation via the output connection points of the microfluidic structure of the card.

Afin de standardiser la carte et sa structure micro-fluidique par rapport à plusieurs applications, la structure micro-fluidique de la carte doit pouvoir être modifiée facilement et adaptée à l'application visée. Cette adaptation doit être réalisée facilement et sans risque de dysfonctionnement, en respectant notamment l'étanchéité du dispositif.In order to standardize the card and its microfluidic structure with respect to several applications, the microfluidic structure of the card must be easily modified and adapted to the intended application. This adaptation must be carried out easily and without risk of malfunction, in particular by respecting the tightness of the device.

La demande de brevet US2010/322826A1 décrit un principe de connexion micro-fluidique par effet magnétique. Ce document est simplement limité à ce principe de connexion magnétique et la solution proposée n'est pas forcément applicable à un dispositif micro-fluidique qui comporte à la fois une carte micro-fluidique et un composant micro-fluidique indépendant et adaptable sur la carte.The patent application US2010 / 322826A1 discloses a micro-fluidic connection principle by magnetic effect. This document is simply limited to this principle of magnetic connection and the proposed solution is not necessarily applicable to a microfluidic device which comprises both a micro-fluidic card and a microfluidic component independent and adaptable on the card.

Le but de l'invention est de proposer un dispositif micro-fluidique dont la structure micro-fluidique peut être modifiée aisément et donc adaptée à une application particulière.The object of the invention is to provide a microfluidic device whose microfluidic structure can be easily modified and thus adapted to a particular application.

Exposé de l'inventionPresentation of the invention

Ce but est atteint par un dispositif micro-fluidique tel que défini en revendication 1.This object is achieved by a microfluidic device as defined in claim 1.

Des particularités du dispositif sont définies dans les revendications secondaires 2 à 11.Features of the device are defined in the secondary claims 2 to 11.

Brève description des figuresBrief description of the figures

D'autres caractéristiques et avantages vont apparaître dans la description détaillée qui suit, faite en regard des dessins annexés dans lesquels :

  • La figure 1 représente, vu en éclaté et en perspective, le dispositif micro-fluidique de l'invention.
  • La figure 2 représente, vu de dessus et assemblé, le dispositif micro-fluidique de l'invention.
  • Les figure 3A et 3B représentent, vu suivant une coupe transversale, le dispositif micro-fluidique de l'invention, respectivement avec le composant micro-fluidique non assemblé sur la carte micro-fluidique et avec le composant micro-fluidique assemblé sur la carte micro-fluidique.
  • Les figure 4A et 4B représentent, respectivement en vue de dessus et en vue de côté, le joint plat employé dans le dispositif micro-fluidique de l'invention.
Other features and advantages will appear in the detailed description which follows, made with reference to the appended drawings in which:
  • The figure 1 represents, seen exploded and in perspective, the microfluidic device of the invention.
  • The figure 2 represents, seen from above and assembled, the microfluidic device of the invention.
  • The Figure 3A and 3B represent, seen in cross-section, the microfluidic device of the invention, respectively with the microfluidic component unassembled on the microfluidic card and with the microfluidic component assembled on the microfluidic card.
  • The Figure 4A and 4B represent, respectively in top view and in side view, the flat gasket used in the microfluidic device of the invention.

Sur les figures annexées, les chemins fluidiques sont représentés de manière simplifiée et n'ont pas forcément de correspondance avec la réalité.In the accompanying figures, the fluidic paths are represented in a simplified manner and do not necessarily correspond to reality.

Description détaillée d'au moins un mode de réalisationDetailed description of at least one embodiment

Dans la suite de la description, les termes "inférieur" et "supérieur" sont à comprendre en prenant comme référence un axe vertical.In the remainder of the description, the terms "lower" and "higher" are to be understood by taking as a reference a vertical axis.

En référence à la figure 1, le dispositif micro-fluidique 1 comporte une carte micro-fluidique 2. Cette carte 2 a par exemple les dimensions d'une carte de crédit. Elle comporte une structure micro-fluidique, dite première structure micro-fluidique, qui comporte plusieurs éléments micro-fluidiques. Par éléments micro-fluidiques, on entend par exemple :

  • des points de connexion micro-fluidique d'entrée par lesquels peut être injecté un fluide dans la structure micro-micro-fluidique ;
  • des points de connexion micro-fluidique de sortie par lesquels peut être extrait un fluide de la structure micro-fluidique ;
  • des canaux micro-fluidiques formant un réseau plus ou moins complexe entre un ou plusieurs points de connexion micro-fluidique d'entrée et un ou plusieurs points de connexion micro-fluidique sortie ;
  • des chambres de réaction ou de stockage ;
  • des micro-vannes...
With reference to the figure 1 , the microfluidic device 1 comprises a micro-fluidic card 2. This card 2 has for example the dimensions of a credit card. It comprises a microfluidic structure, called the first microfluidic structure, which comprises several microfluidic elements. By micro-fluidic elements is meant for example:
  • micro-fluidic inlet connection points through which a fluid can be injected into the micro-microfluidic structure;
  • micro-fluidic outlet connection points through which fluid can be extracted from the microfluidic structure;
  • micro-fluidic channels forming a more or less complex network between one or more micro-fluidic inlet connection points and one or more microfluidic connection points output;
  • reaction or storage chambers;
  • micro-valves ...

La carte micro-fluidique 2 est par exemple réalisée dans un matériau plastique tel que le PMMA (Polyméthacrylate de Méthyle), le COC ("Cyclic Olefin Copolymer" - polymère de cyclooléfine) ou autre matériau similaire.The micro-fluidic card 2 is for example made of a plastic material such as PMMA (polymethyl methacrylate), COC ("Cyclic Olefin Copolymer" - cycloolefin polymer) or other similar material.

De manière non limitative, la carte micro-fluidique est réalisée par assemblage d'au moins deux couches 20, 21 collées sur l'une l'autre et dites première couche 20 et deuxième couche 21, visibles sur la figure 1. De manière non limitative, les éléments micro-fluidiques définis ci-dessus peuvent être formés de différentes manières, comme par exemple :

  • Sous la forme d'une empreinte réalisée sur la surface d'une couche qui est ensuite recouverte par l'autre couche ;
  • En superposant une première empreinte réalisée sur la surface de la première couche et une deuxième empreinte réalisée sur la surface de la deuxième couche, la première empreinte étant située en vis-à-vis de la deuxième empreinte pour former l'élément fluidique.
Without limitation, the micro-fluidic card is made by assembling at least two layers 20, 21 bonded to each other and said first layer 20 and second layer 21, visible on the figure 1 . Without limitation, the micro-fluidic elements defined above can be formed in different ways, for example:
  • In the form of an imprint made on the surface of a layer which is then covered by the other layer;
  • By superimposing a first imprint made on the surface of the first layer and a second imprint made on the surface of the second layer, the first cavity being located vis-à-vis the second cavity to form the fluidic element.

Des éléments micro-fluidiques peuvent notamment être formés sur deux niveaux de profondeur de la carte, en étant réalisés de manière distincte dans la première couche 20 et dans la deuxième couche 21 de la carte.Micro-fluidic elements may in particular be formed on two depth levels of the card, being made separately in the first layer 20 and in the second layer 21 of the card.

Les empreintes sont par exemple réalisées par usinage de la surface de la couche correspondante.The impressions are for example made by machining the surface of the corresponding layer.

La fabrication d'une telle carte micro-fluidique 2 étant bien connue, celle-ci n'est pas détaillée dans la présente demande.The manufacture of such a microfluidic card 2 is well known, it is not detailed in the present application.

La carte 2 ainsi formée comporte une face dite supérieure 200, une face dite inférieure 210 et une tranche 220 formant son épaisseur. Les éléments micro-fluidiques sont formés dans l'épaisseur de la carte 2 et accessibles par des points de connexion micro-fluidique 201 d'entrée et de sortie qui débouchent et qui sont accessibles sur la face supérieure 200 et/ou sur la face inférieure 210 de la carte 2. Sur les figures 1 et 2, la carte comporte également des canaux micro-fluidiques 202 reliant différents points de connexion micro-fluidique 201 de manière à former un réseau d'éléments micro-fluidiques interconnectés.The card 2 thus formed comprises a so-called upper face 200, a so-called lower face 210 and a wafer 220 forming its thickness. The microfluidic elements are formed in the thickness of the card 2 and accessible by micro-fluidic connection points 201 of inlet and outlet which open and which are accessible on the upper face 200 and / or on the lower face 210 of the map 2. On Figures 1 and 2 , the card also comprises microfluidic channels 202 connecting different points of micro-fluidic connection 201 so as to form an interconnected network of micro-fluidic elements.

Sur l'une de ses faces, par exemple la face supérieure 200 (comme représenté sur les figures 1 et 2), la carte 2 comporte au moins une surface d'appui, dite première surface d'appui formant un emplacement spécifique destiné à recevoir un composant micro-fluidique 3 amovible tel que décrit ci-dessous.On one of its faces, for example the upper face 200 (as represented on the Figures 1 and 2 ), the card 2 comprises at least one bearing surface, said first bearing surface forming a specific location for receiving a removable microfluidic component 3 as described below.

Selon l'invention, le dispositif micro-fluidique 1 comporte en effet un composant micro-fluidique 3 destiné à être assemblé sur la carte 2. Ce composant 3 comporte par exemple deux faces principales opposées, dites face supérieure et face inférieure, séparées entre elles par l'épaisseur du composant, formant sa tranche. Ce composant présente par exemple une forme générale parallélépipédique. Il comporte également sur sa face inférieure une surface, dite deuxième surface d'appui, destinée à venir en vis-à-vis de la première surface d'appui réalisée sur la carte 2 et suivant un plan parallèle à cette première surface d'appui.According to the invention, the microfluidic device 1 comprises in fact a microfluidic component 3 intended to be assembled on the card 2. This component 3 comprises for example two opposite main faces, said upper face and lower face, separated from each other by the thickness of the component, forming its edge. This component has for example a parallelepipedic general shape. It also comprises on its underside a surface, said second bearing surface, intended to come opposite the first bearing surface formed on the card 2 and in a plane parallel to this first bearing surface. .

Ce composant micro-fluidique 3 comporte également une structure micro-fluidique, dite deuxième structure micro-fluidique, composé d'éléments micro-fluidiques tels que ceux donnés en exemple ci-dessus. Sur les figures 1 et 2, le composant micro-fluidique 3 comporte des points de connexion micro-fluidique 311 et des canaux micro-fluidiques 312 reliant les points de connexion micro-fluidique 311 de manière à former un réseau d'éléments micro-fluidiques interconnectés.This microfluidic component 3 also comprises a microfluidic structure, called the second microfluidic structure, composed of microfluidic elements such as those exemplified above. On the Figures 1 and 2 the microfluidic component 3 comprises microfluidic connection points 311 and microfluidic channels 312 connecting the microfluidic connection points 311 so as to form an interconnected network of microfluidic elements.

Ce composant micro-fluidique 3 est amovible par rapport à la carte 2. Lorsque le composant 3 est assemblé sur la carte 2, la deuxième structure micro-fluidique apportée par le composant 3 est connectée à la première structure micro-fluidique de la carte 2 par l'intermédiaire de points de connexion disponibles sur la carte et reliés à la structure micro-fluidique de la carte. Ainsi des points de connexion micro-fluidique 201 de la carte sont connectés à des points de connexion micro-fluidique 311 du composant pour interconnecter les deux structures micro-fluidiques.This microfluidic component 3 is removable relative to the card 2. When the component 3 is assembled on the card 2, the second microfluidic structure provided by the component 3 is connected to the first microfluidic structure of the card 2 via connection points available on the board and connected to the micro-fluidic structure of the board. Thus micro-fluidic connection points 201 of the card are connected to microfluidic connection points 311 of the component for interconnecting the two microfluidic structures.

Comme le composant 3 est amovible, celui-ci peut être remplacé par un composant 3 ayant la même structure micro-fluidique ou choisi avec une structure micro-fluidique spécifique en fonction de l'application à laquelle est destiné le dispositif micro-fluidique 1 de l'invention.As the component 3 is removable, it can be replaced by a component 3 having the same microfluidic structure or chosen with a specific microfluidic structure depending on the application for which the microfluidic device 1 is intended. the invention.

Avantageusement, le composant micro-fluidique 3 du dispositif de l'invention se présente en deux parties. Il comporte une première partie 30 qui porte des moyens d'assemblage du composant 3 sur la carte 2 et une deuxième partie 31 qui comporte la structure micro-fluidique du composant. Les deux parties 30, 31 peuvent être réalisées dans des matériaux identiques ou différents. La première partie est agencée pour maintenir la deuxième partie en position sur la carte.Advantageously, the microfluidic component 3 of the device of the invention is in two parts. It comprises a first part 30 which carries assembly means of the component 3 on the card 2 and a second part 31 which comprises the micro-fluidic structure of the component. The two parts 30, 31 can be made of identical or different materials. The first part is arranged to keep the second part in position on the map.

L'architecture en deux parties du composant 3 lui permet avantageusement de séparer sa première partie 30 dédiée à la fixation mécanique de sa deuxième partie 31 dédiée à l'application micro-fluidique. Il est ainsi possible de conserver la première partie 30 du composant 3 tout en modifiant sa structure micro-fluidique, simplement en remplaçant sa deuxième partie 31 par une autre ayant une structure micro-fluidique identique ou différente.The two-part architecture of the component 3 advantageously enables it to separate its first part 30 dedicated to the mechanical fixing of its second part 31 dedicated to the microfluidic application. It is thus possible to keep the first part 30 of the component 3 while modifying its microfluidic structure, simply by replacing its second part 31 with another having an identical or different microfluidic structure.

La première partie 30 du composant micro-fluidique se présente par exemple sous la forme d'un cadre de forme rectangulaire présentant une surface supérieure et une surface inférieure. La deuxième partie 31 du composant micro-fluidique 3 se présente par exemple sous la forme d'une plaque ou puce micro-fluidique venant se positionner en appui contre la surface inférieure du cadre formé par la première partie 30 de manière être maintenue par celle-ci en vis-à-vis de la face supérieure 200 de la carte micro-fluidique 2. Cette puce micro-fluidique est par exemple réalisée dans un matériau comme le silicium. Sa structure micro-fluidique est créée par des processus connus.The first part 30 of the microfluidic component is for example in the form of a rectangular frame having an upper surface and a lower surface. The second portion 31 of the microfluidic component 3 is for example in the form of a micro-fluidic plate or chip which is positioned in abutment against the lower surface of the frame formed by the first part 30 so as to be held by the latter. ci-vis-à-vis the upper face 200 of the microfluidic card 2. This microfluidic chip is for example made of a material such as silicon. Its microfluidic structure is created by known processes.

Le dispositif micro-fluidique 1 de l'invention comporte au moins un joint d'étanchéité 4, avantageusement plat, destiné à être positionné entre la première surface d'appui et la deuxième surface d'appui décrites ci-dessus, de manière à réaliser l'étanchéité entre la carte 2 et le composant 3. Dans l'architecture décrite ci-dessus et représentée sur les figures, ce joint d'étanchéité est pris en sandwich entre la plaque ou puce formée par la deuxième partie 31 du composant micro-fluidique 3 et la face supérieure 200 de la carte micro-fluidique 2.The microfluidic device 1 of the invention comprises at least one advantageously flat gasket 4 intended to be positioned between the first bearing surface and the second bearing surface described above, so as to realize between the card 2 and the component 3. In the architecture described above and shown in the figures, this seal is sandwiched between the plate or chip formed by the second part 31 of the micro-component. fluidic 3 and the upper face 200 of the microfluidic card 2.

Comme représenté sur les figures 4A et 4B, ce joint d'étanchéité se compose par exemple d'une bande ou d'une plaque, réalisée en matériau souple tel que par exemple du caoutchouc ou de la silicone. De manière avantageuse, le joint en silicone présente la particularité de très bien adhérer à une surface en silicium comme celle qui compose la puce de la deuxième partie 31 du composant micro-fluidique 3. De plus, un tel joint en silicone est repositionnable à volonté sur la puce en silicium.As shown on Figures 4A and 4B , This seal consists for example of a strip or a plate, made of flexible material such as for example rubber or silicone. Advantageously, the silicone seal has the particularity of adhering very well to a silicon surface such as that which composes the chip of the second portion 31 of the microfluidic component 3. In addition, such a silicone seal is repositionable at will. on the silicon chip.

Le joint d'étanchéité 4 comporte plusieurs orifices 40 réalisés à travers l'épaisseur de la bande et destinés chacun à se positionner dans l'axe d'un point de connexion micro-fluidique 201 de la carte et d'un point de connexion micro-fluidique 311 du composant de manière à assurer l'étanchéité de la liaison entre les deux points et donc, de manière plus générale, entre la structure micro-fluidique de la carte 2 et la structure micro-fluidique du composant 3 lorsque les deux structures sont interconnectées. Comme représenté sur les figures annexées, le dispositif 1 comporte par exemple deux joints d'étanchéité de ce type pour chaque composant micro-fluidique 3 afin d'étanchéifier des points de connexion micro-fluidique d'entrée et des points de connexion micro-fluidique de sortie. Ceux-ci pourront être identiques ou différents, en termes de dimensions ou dans la position des orifices... Les orifices réalisés à travers chaque joint d'étanchéité sont positionnés de manière adaptée pour assurer la jonction étanche de plusieurs points de connexion en même temps à l'aide d'un seul joint d'étanchéité. Chaque joint est avantageusement fixé sur la face supérieure 200 de la carte micro-fluidique 2 ou sur la face en vis-à-vis du composant micro-fluidique 3.The seal 4 comprises several orifices 40 made through the thickness of the strip and each intended to be positioned in the axis of a microfluidic connection point 201 of the card and a micro connection point. -fluidic 311 of the component so as to seal the connection between the two points and therefore, more generally, between the micro-fluidic structure of the card 2 and the microfluidic structure of the component 3 when the two structures are interconnected. As shown in the accompanying figures, the device 1 comprises for example two seals of this type for each microfluidic component 3 in order to seal micro-fluidic input connection points and micro-fluidic connection points. Release. These may be identical or different, in terms of dimensions or in the position of the orifices ... The orifices made through each seal are positioned in a suitable manner to ensure the tight junction of several connection points at the same time using a single seal. Each seal is advantageously fixed on the upper face 200 of the microfluidic card 2 or on the face facing the microfluidic component 3.

Selon un aspect avantageux de l'invention, le joint est plat et comporte, sur l'une de ses deux faces planes, un bourrelet 41 ou surépaisseur réalisée dans le sens de l'épaisseur du joint autour de chaque orifice 40 et contre lequel vient s'appliquer la compression.According to an advantageous aspect of the invention, the seal is flat and comprises, on one of its two flat faces, a bead 41 or extra thickness made in the direction of the thickness of the seal around each orifice 40 and against which comes apply compression.

Selon un aspect de l'invention, le joint d'étanchéité 4 peut être rendu solidaire de la carte micro-fluidique 2 ou du composant micro-fluidique 3, avant montage du composant 3 sur la carte 2.According to one aspect of the invention, the seal 4 can be made integral with the microfluidic card 2 or the microfluidic component 3, before mounting the component 3 on the card 2.

Le dispositif 1 comporte des moyens de centrage et d'assemblage du composant micro-fluidique 3 sur la carte micro-fluidique 2. Ces moyens de centrage et d'assemblage sont agencés pour assurer une mise en compression suffisante du joint 4. Ces moyens comportent au moins un dispositif magnétique.The device 1 comprises means for centering and assembling the microfluidic component 3 on the micro-fluidic card 2. These centering and assembling means are arranged to ensure sufficient compression of the seal 4. These means comprise at least one magnetic device.

De manière avantageuse, les moyens de centrage et d'assemblage comportent également un dispositif mécanique coopérant avec ledit dispositif magnétique pour assurer le centrage et l'assemblage du composant sur la carte.Advantageously, the centering and assembly means also comprise a mechanical device cooperating with said magnetic device to ensure the centering and assembly of the component on the card.

Le dispositif magnétique intègre ainsi au moins deux éléments d'assemblage par effet magnétique agencés pour s'attirer par effet magnétique.The magnetic device thus integrates at least two magnetic effect assembly elements arranged to attract by magnetic effect.

Par élément d'assemblage par effet magnétique, on entend un aimant permanent et/ou une pièce en matériau ferromagnétique ou une combinaison des deux, au moins l'un des deux éléments étant un aimant permanent pour assurer un phénomène d'attraction magnétique entre le composant micro-fluidique 3 et la carte micro-fluidique 2. Ces deux éléments sont répartis entre la carte micro-fluidique 2 et le composant micro-fluidique 3 pour assurer l'assemblage. Une polarisation magnétique adaptée du dispositif magnétique permet en outre d'assurer un centrage par effet magnétique. L'effet magnétique généré entre deux éléments d'assemblage par effet magnétique est choisi suffisant pour :

  • Maintenir le composant micro-fluidique 3 dans sa position sur la carte 2, et
  • Assurer une compression de chaque joint 4 et permettre ainsi une connexion fluidique étanche entre la structure micro-fluidique de la carte 2 et la structure micro-fluidique du composant 3.
By magnetic effect joining element is meant a permanent magnet and / or a piece of ferromagnetic material or a combination of both, at least one of the two elements being a permanent magnet to ensure a magnetic attraction phenomenon between the micro-fluidic component 3 and the microfluidic card 2. These two elements are distributed between the micro-fluidic card 2 and the microfluidic component 3 to ensure assembly. A magnetic polarization adapted to the magnetic device also makes it possible to provide a centering effect magnetic. The magnetic effect generated between two magnetic effect assembly elements is chosen to be sufficient for:
  • Keep the micro-fluidic component 3 in its position on the card 2, and
  • Ensure a compression of each gasket 4 and thus allow a tight fluidic connection between the microfluidic structure of the card 2 and the microfluidic structure of the component 3.

Le dispositif mécanique comporte préférentiellement une solution d'assemblage de type mâle/femelle. Il comporte ainsi par exemple des plots 303 et des cavités 203 correspondantes. De manière non limitative, lesdits plots 303 sont par exemple réalisés sur le composant micro-fluidique 3 tandis que les cavités sont formées sur la carte micro-fluidique 2. Lesdites cavités 203 sont agencées sur la face supérieure 200 de la carte, autour de la première surface d'appui, et sont destinées chacune à accueillir un plot 303 correspondant réalisé sur le composant 3. Sur le composant micro-fluidique 3, les plots 303 sont agencés pour positionner et maintenir la deuxième partie du composant en position sur la carte 2. Les plots 303 sont avantageusement formés sur la première partie 30 du composant 3 et sont répartis sur la deuxième surface d'appui du composant micro-fluidique 3. Cette deuxième surface d'appui est réalisée en tout ou partie sur la deuxième partie 31 du composant. La deuxième partie 31 du composant micro-fluidique 3 comporte la structure micro-fluidique du composant 3, c'est-à-dire notamment les points de connexion micro-fluidique 311 d'entrée et/ou de sortie et les canaux micro-fluidiques 312 évoqués ci-dessus. Comme déjà évoqué, ces points de connexion micro-fluidique 311 sont destinés à se connecter sur des points de connexion micro-fluidique 201 d'entrée et/ou de sortie réalisés sur la carte 2 pour connecter la structure micro-fluidique du composant 3 à la structure micro-fluidique de la carte 2. Les plots 303 sont par exemple en forme de cylindre de révolution et les cavités 203 disposent d'une forme femelle correspondante.The mechanical device preferably comprises a male / female type assembly solution. It thus comprises, for example, studs 303 and corresponding cavities 203. In a nonlimiting manner, said pads 303 are for example made on the microfluidic component 3 while the cavities are formed on the micro-fluidic card 2. Said cavities 203 are arranged on the upper face 200 of the card, around the first bearing surface, and are each intended to receive a corresponding pad 303 made on the component 3. On the microfluidic component 3, the pads 303 are arranged to position and maintain the second part of the component in position on the card 2 The studs 303 are advantageously formed on the first part 30 of the component 3 and are distributed on the second bearing surface of the microfluidic component 3. This second bearing surface is made wholly or partly on the second part 31 of the component. The second part 31 of the microfluidic component 3 comprises the microfluidic structure of the component 3, that is to say in particular the micro-fluidic connection points 311 of inlet and / or outlet and the microfluidic channels. 312 mentioned above. As already mentioned, these microfluidic connection points 311 are intended to connect to micro-fluidic connection points 201 of input and / or output made on the card 2 to connect the microfluidic structure of the component 3 to the micro-fluidic structure of the card 2. The pads 303 are for example in the form of a cylinder of revolution and the cavities 203 have a corresponding female shape.

Selon un mode de réalisation particulier illustré par les figures 3A et 3B, le dispositif magnétique comporte plusieurs éléments d'assemblage par effet magnétique tels que des aimants permanents 50, formant chacun, par leur forme, un plot 303 du dispositif mécanique ou logé dans un plot 303 du dispositif mécanique pour associer au plot un effet magnétique. Le dispositif magnétique comporte aussi plusieurs autres éléments d'assemblage par effet magnétique, par exemple aussi des aimants permanents 60, qui sont avantageusement logés dans l'épaisseur de la carte, de manière à présenter une face dans le fond de chaque cavité 203 ou séparée du fond de ladite cavité 203 par une épaisseur de carte à cet endroit. Ces aimants permanents 60 sont par exemple logés dans des espaces ménagés dans la deuxième couche 21 de la carte micro-fluidique 2 alors que les cavités 203 sont réalisées par des orifices qui traversent ou non la première couche 20 de la carte. Selon ce mode de réalisation particulier, les aimants permanents 50 sont positionnés au niveau des quatre coins du composant. Chaque cavité 203 peut en effet être non traversante à travers la première couche 20. Ainsi, chaque aimant permanent 60 se trouve emprisonné dans la deuxième couche 21 de la carte 2 une fois que les deux couches 20, 21 sont assemblées l'une sur l'autre.According to a particular embodiment illustrated by the Figures 3A and 3B , the magnetic device comprises several magnetic assembly elements such as permanent magnets 50, each forming, by their shape, a stud 303 of the mechanical device or housed in a stud 303 of the mechanical device to associate the stud with a magnetic effect. The magnetic device also comprises several other magnetic-effect assembly elements, for example also permanent magnets 60, which are advantageously housed in the thickness of the card, so as to have a face in the bottom of each cavity 203 or separate from the bottom of said cavity 203 by a map thickness at this location. These permanent magnets 60 are for example housed in spaces formed in the second layer 21 of the micro-fluidic card 2 while the cavities 203 are formed by orifices that pass through or not the first layer 20 of the card. According to this particular embodiment, the permanent magnets 50 are positioned at the four corners of the component. Each cavity 203 can indeed be non-through through the first layer 20. Thus, each permanent magnet 60 is trapped in the second layer 21 of the card 2 once the two layers 20, 21 are assembled one on the other. 'other.

De manière avantageuse, lorsque le composant micro-fluidique 3 est assemblé sur la carte micro-fluidique 2 grâce aux deux dispositifs mécanique et magnétique décrits ci-dessus, un entrefer (référencé e sur la figure 3B) est avantageusement laissé entre chaque aimant permanent 50 du composant et l'aimant permanent 60 correspondant de la carte micro-fluidique 2 (ou de manière plus générale entre deux éléments d'assemblage par effet magnétique). Pour cela, Il s'agit par exemple de réaliser chaque cavité 203 avec une profondeur supérieure à la hauteur de chaque plot 303 correspondant du composant. La présence d'entrefers présente l'avantage d'assurer une compression uniforme du joint 4 par l'effet magnétique. L'entrefer peut être réalisé au moins par l'épaisseur de matière présente entre le fond de la cavité 203 réalisée dans la première couche 20 et l'aimant 60 présent au-dessous dans la deuxième couche 21.Advantageously, when the microfluidic component 3 is assembled on the micro-fluidic card 2 by virtue of the two mechanical and magnetic devices described above, an air gap (referenced e on the figure 3B ) is advantageously left between each permanent magnet 50 of the component and the corresponding permanent magnet 60 of the micro-fluidic card 2 (or, more generally, between two magnetic-effect assembly elements). For this, it is for example to make each cavity 203 with a depth greater than the height of each corresponding stud 303 of the component. The presence of air gaps has the advantage of ensuring uniform compression of the seal 4 by the magnetic effect. The gap can be made at least by the thickness of material present between the bottom of the cavity 203 made in the first layer 20 and the magnet 60 present below in the second layer 21.

De manière avantageuse, le bourrelet 41 réalisé sur la surface du joint autour de chacun de ses orifices 40 permet d'assurer l'exercice d'une force maximale sur la surface du bourrelet 41 par l'effet magnétique.Advantageously, the bead 41 made on the surface of the seal around each of its orifices 40 ensures the exercise of a maximum force on the surface of the bead 41 by the magnetic effect.

De manière non limitative, ci-dessous quelques éléments de dimensionnement des moyens de centrage et d'assemblage décrits ci-dessus :

  • Aimant composant de type Néodyme : forme cylindrique - diamètre de 3mm - hauteur 3mm - force d'adhérence de 290g.
  • Aimant carte de type Néodyme : forme parallélépipédique - dimensions 6x5x2mm - force d'adhérence de 600g.
  • Entrefer entre les deux aimants : environ 1 mm.
In a nonlimiting manner, below some elements of dimensioning of the centering and assembly means described above:
  • Neodymium component magnet: cylindrical shape - diameter of 3mm - height 3mm - adhesion strength of 290g.
  • Magnet Neodymium type card: parallelepiped shape - dimensions 6x5x2mm - adhesion strength 600g.
  • Air gap between the two magnets: about 1 mm.

Sur les figures 1 et 2 annexées, de manière non limitative, la carte micro-fluidique 2 est représentée avec deux emplacements distincts pour accueillir deux composants du type décrit ci-dessus sur la même carte.On the Figures 1 and 2 attached, without limitation, the micro-fluidic card 2 is shown with two separate locations to accommodate two components of the type described above on the same card.

On comprend de ce qui précède que la solution de l'invention présente plusieurs avantages, parmi-lesquels :

  • Une facilité d'assemblage du composant sur la carte, notamment grâce à l'utilisation du dispositif magnétique ;
  • Une possibilité de réaliser différentes configurations micro-fluidiques, simplement en remplaçant la puce du composant micro-fluidique 3 ;
  • L'obtention d'une solution fiable, notamment au niveau des connexions micro-fluidiques ;
  • Une réalisation simple, avec un nombre limité de composants ;
It is understood from the above that the solution of the invention has several advantages, among which:
  • Ease of assembly of the component on the card, in particular through the use of the magnetic device;
  • A possibility of making different microfluidic configurations simply by replacing the chip of the microfluidic component 3;
  • Obtaining a reliable solution, especially at the level of the microfluidic connections;
  • A simple realization, with a limited number of components;

Claims (11)

Dispositif micro-fluidique comprenant : - Une carte micro-fluidique (2) qui comporte : ∘ Une première structure micro-fluidique comprenant des premiers points de connexion micro-fluidique destinés à être traversés par un fluide, ∘ Une première surface d'appui, ∘ Des premiers moyens d'assemblage et de centrage d'un composant micro-fluidique (3) sur ladite surface d'appui, - Un composant micro-fluidique (3) qui comporte : ∘ Une deuxième structure micro-fluidique comprenant des deuxièmes points de connexion micro-fluidique (311) destinés à être traversés par un fluide et des canaux micro-fluidiques (312) reliant ses deuxièmes points de connexion micro-fluidique (311) de manière à former un réseau d'éléments micro-fluidiques interconnectés, et destinée à se connecter à la première structure micro-fluidique de la carte micro-fluidique (2) pour connecter lesdits premiers points de connexion micro-fluidique avec lesdits deuxièmes points de connexion micro-fluidique, ∘ Des deuxièmes moyens d'assemblage et de centrage agencés pour coopérer avec lesdits premiers moyens d'assemblage et de centrage de la carte micro-fluidique (2), - Un joint d'étanchéité (4) apte à assurer une connexion étanche entre la première structure micro-fluidique et la deuxième structure micro-fluidique, Caractérisé en ce que : - Lesdits premiers moyens d'assemblage et de centrage comportent au moins un premier élément d'assemblage par effet magnétique fixé sur ladite carte micro-fluidique (2) et agencé pour coopérer par effet magnétique avec un deuxième élément d'assemblage par effet magnétique dudit composant micro-fluidique pour compresser ledit joint d'étanchéité (4) apte à assurer une connexion étanche entre la première structure micro-fluidique et la deuxième structure micro-fluidique. Micro-fluidic device comprising: A micro-fluidic card (2) which comprises: A first microfluidic structure comprising first micro-fluidic connection points intended to be traversed by a fluid, ∘ A first support surface, ∘ First means for assembling and centering a microfluidic component (3) on said bearing surface, A microfluidic component (3) comprising: A second microfluidic structure comprising second micro-fluidic connection points (311) to be traversed by a fluid and microfluidic channels (312) connecting its second microfluidic connection points (311) so as to forming an array of interconnected microfluidic elements, and for connecting to the first microfluidic structure of the micro-fluidic board (2) for connecting said first microfluidic connection points with said second micro-fluidic connection points; fluidic, Second assembly and centering means arranged to cooperate with said first assembly and centering means of the microfluidic card (2), A seal (4) capable of ensuring a tight connection between the first microfluidic structure and the second microfluidic structure, Characterized in that : Said first assembly and centering means comprise at least one first magnetic effect fastening element fixed on said micro-fluidic card (2) and arranged to cooperate by magnetic effect with a second magnetic-effect assembly element; microfluidic component for compressing said seal (4) adapted to ensure a tight connection between the first microfluidic structure and the second microfluidic structure. Dispositif micro-fluidique selon la revendication 1, caractérisé en ce que lesdits premiers moyens d'assemblage et de centrage comportent au moins un premier organe mécanique destiné à coopérer avec un deuxième organe mécanique desdits deuxièmes moyens d'assemblage et de centrage dudit composant micro-fluidique.Micro-fluidic device according to claim 1, characterized in that said first assembly and centering means comprise at least one first mechanical member intended to cooperate with a second mechanical member of said second means for assembling and centering said micro-component. fluidics. Dispositif micro-fluidique selon la revendication 2, caractérisé en ce que ledit premier organe mécanique comporte au moins une cavité (203) réalisée dans la carte.Micro-fluidic device according to claim 2, characterized in that said first mechanical member comprises at least one cavity (203) made in the card. Dispositif micro-fluidique selon la revendication 3, caractérisé en ce que ledit premier élément d'assemblage par effet magnétique est logé au fond de ladite cavité (203).Micro-fluidic device according to claim 3, characterized in that said first magnetic effect connecting element is housed at the bottom of said cavity (203). Dispositif micro-fluidique selon l'une des revendications 1 à 4, caractérisé en ce que ledit premier élément d'assemblage par effet magnétique est un aimant permanent (60).Micro-fluidic device according to one of claims 1 to 4, characterized in that said first magnetic effect connecting element is a permanent magnet (60). Dispositif micro-fluidique selon la revendication 3, caractérisé en ce que ledit deuxième organe mécanique comporte au moins un plot (303) destiné à coopérer selon un fonctionnement mâle/femelle avec ladite cavité (203) réalisée sur la carte micro-fluidique (2).Micro-fluidic device according to claim 3, characterized in that said second mechanical member comprises at least one stud (303) intended to cooperate in a male / female operation with said cavity (203) made on the microfluidic card (2) . Dispositif micro-fluidique selon la revendication 6, caractérisé en ce que ledit deuxième élément d'assemblage par effet magnétique est logé dans ledit plot (303).Microfluidic device according to claim 6, characterized in that said second magnetic assembly element is housed in said stud (303). Dispositif micro-fluidique selon la revendication 7, caractérisé en ce que ledit deuxième élément d'assemblage par effet magnétique est un aimant permanent (50).Micro-fluidic device according to claim 7, characterized in that said second magnetic effect connecting element is a permanent magnet (50). Dispositif micro-fluidique selon l'une des revendications 1 à 8, caractérisé en ce que le composant micro-fluidique (3) comporte deux parties séparables l'une par rapport à l'autre, une première partie (30) comportant lesdits moyens d'assemblage et de centrage sur ladite carte et une deuxième partie (31) comportant ladite deuxième structure micro-fluidique.Micro-fluidic device according to one of claims 1 to 8, characterized in that the microfluidic component (3) comprises two parts separable from each other, a first portion (30) comprising said means assembly and centering on said board and a second portion (31) having said second microfluidic structure. Dispositif selon l'une des revendications 1 à 9, caractérisé en ce que le joint d'étanchéité (4) se présente sous la forme d'une bande à travers laquelle sont percés plusieurs orifices (40), destinés chacun à effectuer une jonction étanche entre un point de connexion micro-fluidique de la première structure micro-fluidique et un point de connexion micro-fluidique de la deuxième structure micro-fluidique.Device according to one of claims 1 to 9, characterized in that the seal (4) is in the form of a strip through which are drilled several orifices (40), each intended to make a tight connection between a microfluidic connection point of the first microfluidic structure and a microfluidic connection point of the second microfluidic structure. Dispositif selon la revendication 10, caractérisé en ce que le joint d'étanchéité (4) comporte un bourrelet (41) réalisé autour de chacun de ses orifices (40).Device according to claim 10, characterized in that the seal (4) comprises a bead (41) formed around each of its orifices (40).
EP18161894.3A 2017-03-17 2018-03-15 Microfluidic device Active EP3375523B1 (en)

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