EP3262216A4 - Banque de fragments variables monocaténaires présentés sur phage - Google Patents

Banque de fragments variables monocaténaires présentés sur phage Download PDF

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Publication number
EP3262216A4
EP3262216A4 EP16756185.1A EP16756185A EP3262216A4 EP 3262216 A4 EP3262216 A4 EP 3262216A4 EP 16756185 A EP16756185 A EP 16756185A EP 3262216 A4 EP3262216 A4 EP 3262216A4
Authority
EP
European Patent Office
Prior art keywords
phage
chain variable
variable fragment
fragment library
displayed single
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP16756185.1A
Other languages
German (de)
English (en)
Other versions
EP3262216A2 (fr
Inventor
Hong-sen CHEN
Ing-Chien Chen
Chao-Ping Tung
Shin-Chen HOU
Chung-Ming Yu
Chi-Kai Yang
Yi-Kai CHIU
An-Suei Yang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Academia Sinica
Original Assignee
Academia Sinica
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Academia Sinica filed Critical Academia Sinica
Priority to EP19154062.4A priority Critical patent/EP3514181A1/fr
Publication of EP3262216A2 publication Critical patent/EP3262216A2/fr
Publication of EP3262216A4 publication Critical patent/EP3262216A4/fr
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1018—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C07K16/245—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/77—Internalization into the cell
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
EP16756185.1A 2015-02-24 2016-02-23 Banque de fragments variables monocaténaires présentés sur phage Withdrawn EP3262216A4 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP19154062.4A EP3514181A1 (fr) 2015-02-24 2016-02-23 Banque de fragments variables monocaténaires présentés sur phage et anticorps sélectionnés à partir de celle-ci

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562120352P 2015-02-24 2015-02-24
PCT/US2016/019128 WO2016137992A2 (fr) 2015-02-24 2016-02-23 Banque de fragments variables monocaténaires présentés sur phage

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP19154062.4A Division EP3514181A1 (fr) 2015-02-24 2016-02-23 Banque de fragments variables monocaténaires présentés sur phage et anticorps sélectionnés à partir de celle-ci

Publications (2)

Publication Number Publication Date
EP3262216A2 EP3262216A2 (fr) 2018-01-03
EP3262216A4 true EP3262216A4 (fr) 2018-12-05

Family

ID=56789830

Family Applications (2)

Application Number Title Priority Date Filing Date
EP16756185.1A Withdrawn EP3262216A4 (fr) 2015-02-24 2016-02-23 Banque de fragments variables monocaténaires présentés sur phage
EP19154062.4A Withdrawn EP3514181A1 (fr) 2015-02-24 2016-02-23 Banque de fragments variables monocaténaires présentés sur phage et anticorps sélectionnés à partir de celle-ci

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP19154062.4A Withdrawn EP3514181A1 (fr) 2015-02-24 2016-02-23 Banque de fragments variables monocaténaires présentés sur phage et anticorps sélectionnés à partir de celle-ci

Country Status (5)

Country Link
US (1) US10336816B2 (fr)
EP (2) EP3262216A4 (fr)
CN (1) CN107614762B (fr)
TW (1) TWI636060B (fr)
WO (1) WO2016137992A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012030838A2 (pt) * 2010-06-09 2016-11-08 Dana Faber Cancer Inst Inc molécula de ácido nucléico isolada que codifica uma proteína mek1 mutante tendo atividade de mek1, vetor de expressão, célula hospedeira, método de produzir uma prote ína mek1 mutante, proteína mek1 mutante isolada, método de identificar um paciente que tem câncer, método de orimizar o tratamento de um paciente que tem câncer,e, uso de um inibidor de raf e de um inibidor de mek.
EP3509623A4 (fr) * 2016-09-07 2020-06-03 Saksin Lifesciences PVT Ltd Anticorps synthétiques contre le vegf et leurs utilisations
US11434277B2 (en) * 2017-10-20 2022-09-06 Academia Sinica Method for high-throughput screening of neutralizing antibodies, neutralizing antibodies produced therefrom, and uses thereof
CN110079502B (zh) * 2018-02-07 2020-11-06 阿思科力(苏州)生物科技有限公司 Pd-l1 car-nk细胞及其制备和应用
CN109929835A (zh) * 2019-03-27 2019-06-25 中国人民解放军第四军医大学 一种鼠单克隆抗体功能轻链可变区基因的扩增方法
US20230168249A1 (en) * 2020-05-06 2023-06-01 Academia Sinica Recombinant antibodies, kits comprising the same, and uses thereof
TW202204379A (zh) * 2020-06-08 2022-02-01 中央研究院 用以篩選抗體片段的方法、由此製備的重組抗體及其用途
EP4352092A2 (fr) * 2021-06-08 2024-04-17 Academia Sinica Banque de fragments variables à chaîne unique présentés sur phage pour la sélection de fragments d'anticorps spécifiques de la mésothéline

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005044853A2 (fr) * 2003-11-01 2005-05-19 Genentech, Inc. Anticorps anti-vegf

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE286510T1 (de) * 1993-09-07 2005-01-15 Smithkline Beecham Corp In der behandlung von il4 auslösenden krankheiten nützliche rekombinante il4 antikörper
US7244826B1 (en) * 1998-04-24 2007-07-17 The Regents Of The University Of California Internalizing ERB2 antibodies
JP2002536968A (ja) * 1999-01-29 2002-11-05 イムクローン システムズ インコーポレイティド Ｋｄｒに特異的な抗体およびその使用
WO2003099999A2 (fr) * 2002-05-20 2003-12-04 Abmaxis, Inc. Production et selection d'une banque de proteines dans de la silice
US8148085B2 (en) * 2006-05-15 2012-04-03 Sea Lane Biotechnologies, Llc Donor specific antibody libraries
TWI506138B (zh) * 2009-08-11 2015-11-01 Academia Sinica 生產雙硫鍵穩固之單鏈抗體之方法
WO2011137245A2 (fr) * 2010-04-30 2011-11-03 Esperance Pharmaceuticals, Inc. Conjugués ligand her2/neu (récepteur 2 du facteur de croissance épidermique humain)-peptide lytique et méthodes d'utilisation associées
AU2012245073B2 (en) * 2011-04-21 2016-02-11 Garvan Institute Of Medical Research Modified variable domain molecules and methods for producing and using them b
WO2013166604A1 (fr) * 2012-05-10 2013-11-14 Zymeworks Inc. Hybrides d'anticorps monovalents à bras unique et leurs utilisations

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005044853A2 (fr) * 2003-11-01 2005-05-19 Genentech, Inc. Anticorps anti-vegf

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PANSRI POTJAMAS ET AL: "A compact phage display human scFv library for selection of antibodies to a wide variety of antigens", BMC BIOTECHNOLOGY, BIOMED CENTRAL LTD. LONDON, GB, vol. 9, no. 1, 29 January 2009 (2009-01-29), pages 6, XP021049493, ISSN: 1472-6750, DOI: 10.1186/1472-6750-9-6 *

Also Published As

Publication number Publication date
WO2016137992A3 (fr) 2016-10-27
CN107614762A (zh) 2018-01-19
TWI636060B (zh) 2018-09-21
CN107614762B (zh) 2022-08-05
US20180009877A1 (en) 2018-01-11
EP3514181A1 (fr) 2019-07-24
EP3262216A2 (fr) 2018-01-03
US10336816B2 (en) 2019-07-02
TW201632547A (zh) 2016-09-16
WO2016137992A2 (fr) 2016-09-01

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Ipc: C40B 50/06 20060101ALI20180724BHEP

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