EP3230438C0 - N-glykolisierung - Google Patents

N-glykolisierung

Info

Publication number
EP3230438C0
EP3230438C0 EP15822904.7A EP15822904A EP3230438C0 EP 3230438 C0 EP3230438 C0 EP 3230438C0 EP 15822904 A EP15822904 A EP 15822904A EP 3230438 C0 EP3230438 C0 EP 3230438C0
Authority
EP
European Patent Office
Prior art keywords
glycolisation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP15822904.7A
Other languages
English (en)
French (fr)
Other versions
EP3230438B1 (de
EP3230438A2 (de
Inventor
Henrik Clausen
Zhang Yang
Adnan Fevzi Halim
Eric Bennett
Carsten Behrens
Malene Bech Vester-Christensen
Shamim Herbert Rahman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Københavns Universitet
Danmarks Tekniske Universitet
Original Assignee
Københavns Universitet
Danmarks Tekniske Universitet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Københavns Universitet, Danmarks Tekniske Universitet filed Critical Københavns Universitet
Priority to EP24217236.9A priority Critical patent/EP4502167A3/de
Publication of EP3230438A2 publication Critical patent/EP3230438A2/de
Application granted granted Critical
Publication of EP3230438B1 publication Critical patent/EP3230438B1/de
Publication of EP3230438C0 publication Critical patent/EP3230438C0/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
    • C07K16/464—Igs containing CDR-residues from one specie grafted between FR-residues from another
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/24—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/724—Glycosyltransferases (EC 2.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
EP15822904.7A 2014-12-12 2015-12-11 N-glykolisierung Active EP3230438B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP24217236.9A EP4502167A3 (de) 2014-12-12 2015-12-11 N-glykosylierung

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462091056P 2014-12-12 2014-12-12
US201562193403P 2015-07-16 2015-07-16
PCT/DK2015/050391 WO2016091268A2 (en) 2014-12-12 2015-12-11 N-glycosylation

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP24217236.9A Division EP4502167A3 (de) 2014-12-12 2015-12-11 N-glykosylierung

Publications (3)

Publication Number Publication Date
EP3230438A2 EP3230438A2 (de) 2017-10-18
EP3230438B1 EP3230438B1 (de) 2024-12-04
EP3230438C0 true EP3230438C0 (de) 2024-12-04

Family

ID=55079928

Family Applications (2)

Application Number Title Priority Date Filing Date
EP15822904.7A Active EP3230438B1 (de) 2014-12-12 2015-12-11 N-glykolisierung
EP24217236.9A Pending EP4502167A3 (de) 2014-12-12 2015-12-11 N-glykosylierung

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP24217236.9A Pending EP4502167A3 (de) 2014-12-12 2015-12-11 N-glykosylierung

Country Status (4)

Country Link
US (4) US10858671B2 (de)
EP (2) EP3230438B1 (de)
ES (1) ES3003873T3 (de)
WO (1) WO2016091268A2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017008982A1 (en) * 2015-07-16 2017-01-19 University Of Copenhagen Production of n-glycoproteins for enzyme assisted glycomodification
SG11201805170XA (en) 2015-12-18 2018-07-30 Tega Therapeutics Inc Cellular glycosaminoglycan compositions and methods of making and using
US20190330601A1 (en) * 2016-05-13 2019-10-31 University Of Copenhagen A cell-based array platform
ES2982085T3 (es) 2016-06-20 2024-10-14 Octapharma Ag Medios y métodos para modificar alelos múltiples
AU2017378500A1 (en) * 2016-12-16 2019-07-04 Tega Therapeutics, Inc. In vitro heparin and heparan sulfate compositions and methods of making and using
EP3717640A1 (de) 2017-11-28 2020-10-07 Danmarks Tekniske Universitet Glycosylierung von proteinen
JP2021528074A (ja) * 2018-06-20 2021-10-21 マサチューセッツ インスティテュート オブ テクノロジー 特徴的なグリカン構造を有するプロテオグリカンを糖鎖工学的に改変する方法
CA3106651A1 (en) * 2018-07-18 2020-01-23 Stephen YOO Treatment of mucopolysaccharidosis i with fully-human glycosylated human alpha-l-iduronidase (idus)
US11708569B2 (en) 2018-08-29 2023-07-25 University Of Copenhagen Modified recombinant lysosomal alpha-galactosidase A and aspartylglucoaminidase having low mannose-6-phosphate and high sialic acid
CA3166726A1 (en) * 2020-01-07 2021-07-15 Limmatech Biologics Ag Glycoengineering using leishmania cells
WO2022034068A1 (en) 2020-08-10 2022-02-17 Inbiose N.V. Production of a mixture of mammalian milk oligosaccharides by a cell
DK3954778T5 (da) 2020-08-10 2024-08-05 Inbiose Nv Produktion af en blanding af neutrale ikke-fucosylerede oligosaccharider af en celle
WO2022165194A1 (en) * 2021-01-29 2022-08-04 University Of Maryland, College Park Compositions and methods for producing glyco-modified viral antigens
EP4347624A1 (de) 2021-06-04 2024-04-10 University of Copenhagen Peptide mit mucinbindenden eigenschaften
WO2023044365A1 (en) * 2021-09-17 2023-03-23 Doublerainbow Biosciences Inc. Use of cyclodextrin to enhance solubility of substrates and increase enzymatic glycosylation reaction efficiency
CN115058418A (zh) * 2022-01-06 2022-09-16 扬州大学 一种Sec1基因敲除小鼠模型的sgRNA、构建方法及其应用
CN120683174A (zh) * 2024-03-22 2025-09-23 上海药明生物技术有限公司 消除重组蛋白o-木糖化修饰的方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2601882T5 (es) 1999-04-09 2021-06-07 Kyowa Kirin Co Ltd Procedimiento para controlar la actividad de una molécula inmunofuncional
ES2466024T3 (es) 2001-10-10 2014-06-09 Ratiopharm Gmbh Remodelación y glicoconjugación de factor de crecimiento de fibroblastos (FGF)
WO2006079155A1 (en) * 2005-01-25 2006-08-03 Apollo Life Sciences Limited Molecules and chimeric molecules thereof
EP2171052B1 (de) 2007-07-12 2014-08-27 Sangamo BioSciences, Inc. Verfahren und zusammensetzung zur deaktivierung der alpha-1,6-fucosyltransferase-genexpression
WO2009075883A2 (en) * 2007-12-12 2009-06-18 University Of Georgia Research Foundation, Inc. Glycoprotein cancer biomarker
WO2011109600A1 (en) * 2010-03-05 2011-09-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for modifying the glycosylation pattern of a polypeptide
BR112013016447A2 (pt) * 2011-01-06 2019-09-24 Univ Johns Hopkins método de produção de glicoproteínas recombinantes com meia-vida circulatória aumentada em células de mamífero
BR112013033448B1 (pt) 2011-06-30 2022-01-25 Sigma-Aldrich Co. Llc Método para produzir uma proteína recombinante com um padrão de glicosilação semelhante ao de humano
WO2013013013A2 (en) * 2011-07-21 2013-01-24 Alnylam Pharmaceuticals, Inc. Compositions and methods for producing modified glycoproteins
US9464137B2 (en) * 2011-12-13 2016-10-11 Glykos Finland Oy Glycoprotein
US9376484B2 (en) 2012-01-11 2016-06-28 Sigma-Aldrich Co. Llc Production of recombinant proteins with simple glycoforms
KR20220013460A (ko) * 2014-03-04 2022-02-04 시그마-알드리치 컴퍼니., 엘엘씨 바이러스 내성 세포 및 이의 용도

Also Published As

Publication number Publication date
ES3003873T3 (en) 2025-03-11
WO2016091268A3 (en) 2016-09-09
US20210102223A1 (en) 2021-04-08
US20170369905A1 (en) 2017-12-28
WO2016091268A2 (en) 2016-06-16
US20210087587A1 (en) 2021-03-25
US10858671B2 (en) 2020-12-08
EP3230438B1 (de) 2024-12-04
EP3230438A2 (de) 2017-10-18
EP4502167A2 (de) 2025-02-05
US20210095317A1 (en) 2021-04-01
EP4502167A3 (de) 2025-06-11

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