EP3146094A4 - Procédé d'inversion de sonde oligonucléotidique pour des réseaux de sondes synthétisés in situ - Google Patents

Procédé d'inversion de sonde oligonucléotidique pour des réseaux de sondes synthétisés in situ Download PDF

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Publication number
EP3146094A4
EP3146094A4 EP15796731.6A EP15796731A EP3146094A4 EP 3146094 A4 EP3146094 A4 EP 3146094A4 EP 15796731 A EP15796731 A EP 15796731A EP 3146094 A4 EP3146094 A4 EP 3146094A4
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EP
European Patent Office
Prior art keywords
probe
inversion process
situ synthesized
arrays
oligonucleotide probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP15796731.6A
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German (de)
English (en)
Other versions
EP3146094A1 (fr
EP3146094B1 (fr
Inventor
Glenn Mcgall
Vijay Singh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centrillion Technology Holdings Corp
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Centrillion Technology Holdings Corp
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Publication of EP3146094A4 publication Critical patent/EP3146094A4/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Structural Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP15796731.6A 2014-05-23 2015-05-22 Procédé d'inversion de sonde oligonucléotidique pour des réseaux de sondes synthétisés in situ Active EP3146094B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462002760P 2014-05-23 2014-05-23
PCT/US2015/032227 WO2015179790A1 (fr) 2014-05-23 2015-05-22 Procédé d'inversion de sonde oligonucléotidique pour des réseaux de sondes synthétisés in situ

Publications (3)

Publication Number Publication Date
EP3146094A1 EP3146094A1 (fr) 2017-03-29
EP3146094A4 true EP3146094A4 (fr) 2017-11-22
EP3146094B1 EP3146094B1 (fr) 2019-08-28

Family

ID=54554851

Family Applications (1)

Application Number Title Priority Date Filing Date
EP15796731.6A Active EP3146094B1 (fr) 2014-05-23 2015-05-22 Procédé d'inversion de sonde oligonucléotidique pour des réseaux de sondes synthétisés in situ

Country Status (4)

Country Link
US (1) US10533216B2 (fr)
EP (1) EP3146094B1 (fr)
CN (1) CN106460232B (fr)
WO (1) WO2015179790A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10533216B2 (en) 2014-05-23 2020-01-14 Centrillion Technology Holdings Corporation Oligonucleotide probe inversion process for in situ synthesized probe arrays
US10689690B2 (en) 2015-08-13 2020-06-23 Centrillion Technology Holdings Corporation Library construction using Y-adapters and vanishing restriction sites
US10695735B2 (en) 2015-08-18 2020-06-30 Centrillion Technology Holdings Corporation Probe inversion process for in situ synthesized probe arrays
EP3133171B1 (fr) * 2015-08-18 2018-10-17 Centrillion Technology Holdings Corporation Procédé d'inversion de sonde pour des ensembles de sondes synthétisés in situ
WO2018102660A1 (fr) * 2016-12-02 2018-06-07 Centrillion Technologies, Inc. Procédé d'inversion de sonde in situ pour construire des réseaux de sondes
US11414687B2 (en) 2017-10-04 2022-08-16 Centrillion Technology Holdings Corporation Method and system for enzymatic synthesis of oligonucleotides
WO2019183272A1 (fr) * 2018-03-21 2019-09-26 Centrillion Technologies, Inc. Procédé et système de fabrication de réseaux de séquençage d'adn
CN114621307A (zh) * 2022-04-12 2022-06-14 中国科学院苏州生物医学工程技术研究所 一种寡核苷酸空间坐标编码方法及其微流控装置

Citations (4)

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Publication number Priority date Publication date Assignee Title
WO1998051698A1 (fr) * 1997-05-14 1998-11-19 Marek Kwiatkowski Synthese d'oligonucleotides
US20040152905A1 (en) * 2003-01-31 2004-08-05 Guzaev Andrei P. Universal building blocks and support media for synthesis of oligonucleotides and their analogs
WO2011106460A2 (fr) * 2010-02-23 2011-09-01 Life Technologies Corporation Procédés pour immobiliser et bloquer avec basculement des acides nucléiques et les séquencer
US20120035115A1 (en) * 2008-09-23 2012-02-09 Alnylam Pharmaceuticals, Inc. Chemical modifications of monomers and oligonucleotides with cycloaddition

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US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US6077668A (en) * 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5959098A (en) * 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
US6077608A (en) 1996-09-19 2000-06-20 Ppg Industries Ohio, Inc. Multilayered coating with powder clear coating and substrates therewith and method
US6262216B1 (en) 1998-10-13 2001-07-17 Affymetrix, Inc. Functionalized silicon compounds and methods for their synthesis and use
US6255476B1 (en) 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
US20070275411A1 (en) 2006-05-25 2007-11-29 Mcgall Glenn H Silane mixtures
AU2005324505B2 (en) 2004-05-13 2009-09-10 Nanobiosym, Inc. Nano-PCR: methods and devices for nucleic acid amplification and detection
DK1828381T3 (da) 2004-11-22 2009-02-23 Peter Birk Rasmussen Templatedirigeret split-and-mix-syntese af småmolekylebiblioteker
US20080305964A1 (en) 2004-12-15 2008-12-11 Roy Bar-Ziv Single-Step Platform for On-Chip Integration of Bio-Molecules
US7749701B2 (en) 2005-08-11 2010-07-06 Agilent Technologies, Inc. Controlling use of oligonucleotide sequences released from arrays
US8114636B2 (en) 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
GB201004292D0 (en) * 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
US20120083417A1 (en) 2010-09-23 2012-04-05 Centrillion Technology Holding Corporation Native-extension parallel sequencing
AU2012212148B8 (en) 2011-02-02 2017-07-06 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
EP2966061B1 (fr) 2011-03-04 2017-05-03 Life Technologies Corporation Composés et procédés de conjugaison de biomolécules
US20130165350A1 (en) * 2011-12-22 2013-06-27 Affymetrix, Inc. Surface linkers for array synthesis
CN102702010B (zh) 2012-05-23 2014-09-17 北京大学 光敏感的功能化固载相及其制备方法和应用
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2015017759A1 (fr) 2013-08-02 2015-02-05 Stc.Unm Séquençage d'adn et analyse de l'épigénome
US10533216B2 (en) 2014-05-23 2020-01-14 Centrillion Technology Holdings Corporation Oligonucleotide probe inversion process for in situ synthesized probe arrays
EP3133171B1 (fr) 2015-08-18 2018-10-17 Centrillion Technology Holdings Corporation Procédé d'inversion de sonde pour des ensembles de sondes synthétisés in situ
WO2018102660A1 (fr) 2016-12-02 2018-06-07 Centrillion Technologies, Inc. Procédé d'inversion de sonde in situ pour construire des réseaux de sondes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998051698A1 (fr) * 1997-05-14 1998-11-19 Marek Kwiatkowski Synthese d'oligonucleotides
US20040152905A1 (en) * 2003-01-31 2004-08-05 Guzaev Andrei P. Universal building blocks and support media for synthesis of oligonucleotides and their analogs
US20120035115A1 (en) * 2008-09-23 2012-02-09 Alnylam Pharmaceuticals, Inc. Chemical modifications of monomers and oligonucleotides with cycloaddition
WO2011106460A2 (fr) * 2010-02-23 2011-09-01 Life Technologies Corporation Procédés pour immobiliser et bloquer avec basculement des acides nucléiques et les séquencer

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BEAUCAGE S L: "STRATEGIES IN THE PREPARATION OF DNA OLIGONUCLEOTIDE ARRAYS FOR DIAGNOSTIC APPLICATIONS", CURRENT MEDICINAL CHEMISTRY : THE NEW INTERNATIONAL JOURNAL FOR TIMELY IN-DEPTH REVIEWS IN MEDICINAL CHEMI, BENTHAM, NL, vol. 8, no. 10, 1 August 2001 (2001-08-01), pages 1213 - 1244, XP009030910, ISSN: 0929-8673 *
KWIATKOWSKI M ET AL: "Inversion of in situ synthesized oligonucleotides; improved reagents for hybridization and primer extension in DNA microarrays", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD, vol. 27, no. 24, 1 January 1999 (1999-01-01), pages 4710 - 4714, XP002964971, ISSN: 0305-1048, DOI: 10.1093/NAR/27.24.4710 *
See also references of WO2015179790A1 *

Also Published As

Publication number Publication date
CN106460232A (zh) 2017-02-22
EP3146094A1 (fr) 2017-03-29
US20170067095A1 (en) 2017-03-09
WO2015179790A1 (fr) 2015-11-26
US10533216B2 (en) 2020-01-14
CN106460232B (zh) 2019-12-24
EP3146094B1 (fr) 2019-08-28

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