EP3075451A1 - Einweg-fluss-chip-vorrichtung - Google Patents

Einweg-fluss-chip-vorrichtung Download PDF

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Publication number
EP3075451A1
EP3075451A1 EP15162040.8A EP15162040A EP3075451A1 EP 3075451 A1 EP3075451 A1 EP 3075451A1 EP 15162040 A EP15162040 A EP 15162040A EP 3075451 A1 EP3075451 A1 EP 3075451A1
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EP
European Patent Office
Prior art keywords
disposable flow
chip device
flow chip
connectors
chip
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP15162040.8A
Other languages
English (en)
French (fr)
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EP3075451B1 (de
Inventor
Pascal Mertens
Isabelle OTE
Thierry Leclipteux
Eric Aussems
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Coris Bioconcept SPRL
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Coris Bioconcept SPRL
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Publication date
Application filed by Coris Bioconcept SPRL filed Critical Coris Bioconcept SPRL
Priority to EP15162040.8A priority Critical patent/EP3075451B1/de
Publication of EP3075451A1 publication Critical patent/EP3075451A1/de
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Publication of EP3075451B1 publication Critical patent/EP3075451B1/de
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers

Definitions

  • the present invention relates to an improved method and device for a genetic amplification and/or a rapid detection of an analyte, possibly present in a fluid sample, especially to a disposable flow chip device, comprising a chip for carrying a microfluidic assay and an interface which is capable, in use, to be easily connected to an external pumping system.
  • the invention is also related to an apparatus comprising the external pumping system.
  • Microfluidic driving systems that are known to the public may be divided into three classes: on-chip mechanical micro-pumps, on-chip electro kinetic micro-pumps and pump systems which are external to the chip.
  • On-chip mechanical micro-pumps present the drawback of being embedded mechanical micro-pumps prepared directly in a chip with the micromachining technology.
  • such devices comprise moveable parts in the chip and comprise a pressure chamber.
  • An intermittent electrostatic driving force is generated between the two-layer structure of the pressure chamber and the two one-way passive check valves positioned in the microfluidic channel are driven in turn. Such an operation generates a pumping force to the fluid.
  • U.S. Pat. No. 5,705,018 discloses a micro peristaltic pump, wherein a series of flexible conductive strips are provided along the inner wall of the micro-channel provided in the chip. When voltage pulses pass over the micro-channel, the flexible conductive strips are pulled upward by electrostatic force generated in turn. A peristaltic phenomenon takes thus place. The fluid in the micro-channel is driven by the driving force of the strips.
  • the mechanical micro-pumps are generally prepared with membranes, valves or gears which are driven by relatively higher powers, such as electric, magnetic or thermal powers.
  • relatively higher powers such as electric, magnetic or thermal powers.
  • Such a requirement involves complicated structure, complicated operations and higher costs.
  • On-chip electro kinetic micro-pumps are non-mechanical micro-pumps. Inside the pumps, there are no moveable elements. Operations of such a micro-pump may be carried out by electro-osmosis, electro-hydrodynamic or electrophoresis.
  • U.S. Pat. No. 5,632,876 discloses an apparatus and methods for controlling fluid flow in micro-channels employing the combination of the electro-osmosis power and the electro-hydrodynamic power.
  • the apparatus comprises a micro-channel provided in a chip and two pairs of electrodes, four in total, are arranged in the micro-channel in turn. A pair of electrodes is deeply put in the micro-channel. When high voltage is applied to the electrodes, fluid adjacent to the electrodes will be carried in a direction reverse to the direction of the electrical current. An electro-hydrodynamic pumping is thus accomplished.
  • An electrode micro-pump is simple in structure, low in manufacture cost but limited in application.
  • solvent must be filled before anything may be driven. It is not possible to introduce samples or reagents into empty channels.
  • the distance over which an electro-hydrodynamic pump can drive a fluid is limited.
  • the electro-hydrodynamic pump can only be applied to nonpolar organic solvents, while the electro-osmosis pump and the electrophoresis pump can only be applied to polar solvents.
  • the driving efficiency of the pumps is highly influenced by the concentration of ions in the solution. When the ion concentration of the solution varies during the reaction, the driving of the solution will become more difficult to control.
  • WO 2014/005969 discloses a flow chip wherein a dynamic constant flow in a closed circuit is applied using an external peristaltic pump or roller pump.
  • the chip comprises two reservoirs, one containing the sample and the other containing a migration buffer. After the introduction of the fluids into the chip, the two reservoirs are connected by a flexible tube forming a loop and adapted to come into contact with one or more rollers of a peristaltic pump. This tube is the interface such that its contact with rollers of the peristaltic pump ensures constant flow dynamics of the sample and buffer in the channel of the flow chip.
  • WO 2014/005969 One problem of the device disclosed in WO 2014/005969 is the interface between the pump and the chip.
  • the use of a flexible tube presents several drawbacks having an impact on the flow dynamics within the chip, drawbacks such as the difficulties encountered by the end user to connect the chip and the pump.
  • the sample and its analyte can be contaminated by the environment or may comprise gas (air) introduced while introducing the sample in the device, leading to unwanted and possibly harmful internal pressure increase.
  • the present invention aims to provide a method and a disposable flow chip device that do not have the drawbacks of the prior art. It particularly aims to provide a flow chip device remaining hermetically closed after the introduction of the fluid sample(s) and allowing gas or air release, which are possibly present in the microfluidic elements of the flow chips before introduction of the fluid sample(s), and therefore avoiding or reducing any interference in the fluids flow inside the device.
  • Another aim of the invention is to propose a suitable interface for connecting efficiently this flow chip device to an external pump.
  • the present invention discloses a disposable flow chip device comprising a microfluidic chip comprising two or more reservoirs being in fluidic communication with a micro-channel, with one or more detection zone(s) and with connectors, preferably luers, a pump cartridge comprising a pipe being in contact, in use, with one or more roller(s) of an external peristaltic pump, and hermetic connectors, preferably luer connectors, complementary to the microfluidic chip connectors, wherein the pipe is in fluid communication with the reservoirs of the microfluidic chip.
  • the present invention further discloses a method to detect and/or quantify one or more analyte(s) possibly present in a biological sample, the method comprising the steps of providing the disposable flow chip device according to the invention, providing one or more analyte(s) comprising a target polynucleotide sequence or polypeptide specific of the said analyte(s), filling the reservoir of the disposable flow chip device with the one or more analyte(s) and submitting the one or more analyte(s) to a fluid flow within the disposable flow chip device.
  • the present invention further discloses an apparatus to detect and/or quantify one or more analyte(s) possibly present in a biological sample, the apparatus comprising one or more disposable flow chip device(s) according to the invention, a holder for holding the disposable flow chip device(s), a peristaltic pump comprising multiple rollers intended to contact the pipe of the pump cartridge, a heating device providing different temperature zones to the disposable flow chip device, a detector for measuring a signal generated on the disposable flow chip device.
  • FIG. 1 An example of the disposable flow chip device 1 according to the invention is represented in figure 1 (bottom view) and figure 2 (top view). It comprises two parts, a microfluidic chip 2 and a pump cartridge 3, which are assembled by connection means, preferably connectors, more preferably connectors hermetic to fluids and/or gas or air, for example luers 12 and luer connectors 10.
  • connection means preferably connectors, more preferably connectors hermetic to fluids and/or gas or air, for example luers 12 and luer connectors 10.
  • the disposable flow chip device 1 is thus hermetically closed when these two parts are assembled.
  • having hermetic connectors 10 and 12 presents the advantage of reducing or eliminating the sample contamination risks during the use of the device.
  • the pump cartridge 3 comprises a pipe 9 (visible in figure 3A ) intended to be, in use, in contact with one or more roller(s) 27 of an external peristaltic pump 4. Therefore, the pipe 9 is in fluid communication with the reservoirs 13 and 14 of the microfluidic chip 2 so that, when the external pump 4 is running, the fluid can flow through the pipe 9 (i.e. through contact between the rollers applied upon the pipe 9) in a continuous manner between the chip 2 and the pump cartridge 3, by means of the action of the rollers of the external pump on the (flexible) pipe 9.
  • the pipe 9 is a tubing, more preferably a flexible tubing.
  • the pump cartridge 3 has a U shape comprising a bridge section and two legs interconnected by the bridge section.
  • the pipe 9 is preferably provided in the plan formed by the pump cartridge 3, from one leg to the other.
  • the pump cartridge 3 is assembled, connected, to the microfluidic chip 2 by means of the bridge section.
  • the pump cartridge 3 preferably comprises one or more pins 7 for plugging the pump cartridge 3 into the microfluidic chip 2 which have complementary holes, or recess, 15, 16, having a size and a shape suitable to receive said pins 7.
  • the pump cartridge 3 preferably comprises one or more cavities 5 suitable to receive one or more connectors, for example luer connectors 10, and at least one cavity 6 suitable to receive a lid 11.
  • the connectors 10 are, in shape and size, complementary to the microfluidic chip connectors 12 (visible on figures 4A , B and C) and allow hermetic connection between the pump cartridge 3 to the microfluidic chip 2.
  • the venting holes 17 present on the microfluidic chip 2 (visible on figures 4A , B and C) allow a gas, for example air, to be released from the microfluidic elements of the microfluidic chip when the reservoirs 13, 14 are filled, allowing thus a better filling of the reservoirs 13 and 14 and also allowing a more constant flow of the fluid within the channel of the chip.
  • the venting holes 17 are hermetically closed by a lid 11 provided on the pump cartridge 3 allowing to have a device hermetically closed and less subject to contaminations.
  • FIG. 3C A cross section view of the pump pipe 9 and the connectors 10 is shown in figure 3C .
  • Figures 4A, 4B and 4C represent different embodiments of the microfluidic chip 2.
  • the microfluidic chip 2 is suitable for amplification and rapid detection of target nucleotide sequences possibly present in a sample. It may combine continuous-flow genetic amplification (PCR) with oligo-chromatography detection by capillary action on a test strip.
  • PCR continuous-flow genetic amplification
  • the chip 2 comprises a first part comprising means for the genetic amplification of nucleic acid sequences specific of an analyte to be detected and possibly present in a fluid sample, submitted thereafter to constant dynamic fluid flow therein, combined with a second part comprising means for the detection of the amplified nucleic acid sequences on a (one or more) sheet-like chromatographic test strip 26 (being in fluidic communication with the channel 18) and allowing thereafter an identification of the corresponding analyte present in the sample submitted to analysis.
  • the two parts are in fluidic communication by means of a connection channel 19.
  • the first part of the microfluidic chip 2 is suitable for genetic amplification (PCR) of one or more target sequence(s) to be detected. It comprises a micro-channel 18 (having a section comprised between about 10 ⁇ m and about 900 ⁇ m) in a serpentine configuration comprising a plurality of loops capable of performing different steps of the genetic amplification process, these loops being in contact, in use, with an external heating means at different zones (or areas) of the loops, preferably two, more preferably three different temperature zones, each zone having a constant temperature.
  • heating means consist of Peltier devices or heating resistances or heating blocks in aluminium and adequate sensors.
  • the micro-channel 18 has a coil configuration, a length and dimensions that may vary, but comprises a certain number of loops able to carry out the different steps required for a suitable genetic amplification (by PCR).
  • the second part of the microfluidic chip 2 comprises a sheet-like chromatographic strip 26 incorporating three zones or regions. These three zones consist of one or more porous membranes allowing the migration by capillary action of a fluid and its components, i.e. the solution comprising the amplified sequences, from a proximal region 20 to a distal region 21.
  • the three zones comprise an application region 22, a detection region 23 and an absorption region 24.
  • the membrane of the application zone 22 comprises or consists of glass fibres or polyester
  • the membrane of the detection zone 23 comprises or consists of nitrocellulose
  • the absorption region 24 comprises or consists of cellulose.
  • Other structures and materials used by a skilled person in the art to improve migration by capillary action in the chromatographic strip 26 or to improve the integration of elements used for the detection on the strip are possible.
  • the microfluidic chip 2 is made of a material selected from the group consisting of glass, quartz or plastic, in particular in polycarbonate or in cyclo olefin polymer, and is obtained by injection molding.
  • the microstructures of the micro-channel are preferably performed by photolithography.
  • the aperture of the micro-channel 18 is optionally adapted for facilitating introduction and preliminary treatment of the sample to be tested and optionally diluted.
  • the microfluidic chip 2 is suitable for the amplification and rapid detection of target nucleotide sequences specific of the analyte present in the fluid sample. It combines continuous-flow genetic amplification (PCR) with oligo-chromatography detection by capillary action on two or more (separated) test strips for multiplexing the number of target nucleotide sequences detected simultaneously and thus for multiplexing the identification of analytes potentially simultaneously present in the fluid sample submitted to analysis.
  • PCR continuous-flow genetic amplification
  • oligo-chromatography detection by capillary action on two or more (separated) test strips for multiplexing the number of target nucleotide sequences detected simultaneously and thus for multiplexing the identification of analytes potentially simultaneously present in the fluid sample submitted to analysis.
  • the microfluidic chip 2 is suitable for the rapid detection of a target analyte, for example any biological analyte, such as an antigenic structure to be detected by immunochromatography, possibly present in a fluid sample without previous genetic amplification (PCR). It combines continuous-flow of a sample solution with immunochromatography detection by capillary action on a test strip.
  • a target analyte for example any biological analyte, such as an antigenic structure to be detected by immunochromatography, possibly present in a fluid sample without previous genetic amplification (PCR).
  • the principle of the analysis of a biological sample is the following.
  • the reservoir 13 of the microfluidic chip 2 is filled with a fluid sample reagent and the reservoir 14 is filled with a buffer.
  • the gas i.e. air, which is displaced by the fluids, the liquid samples, is escaping, released outside the device, via the venting channels 25 to the venting holes 17.
  • the pump cartridge 3 is manually or mechanically plugged into the microfluidic chip 2 by means of complementary connectors 10 and 12.
  • the pump cartridge 3 comprises pins 7 and/or snap fit pins 8 that are complementary to holes 15 and 16 present in proximity of the connectors 12 of the microfluidic chip 2.
  • pins 7 and 8 clamp the pump cartridge 3 against the microfluidic chip 2.
  • This action also clamps the hermetic connectors 10 into the connectors 12 of the microfluidic chip 2, and closes the venting holes 17 by the adequate presence of the lid 11.
  • the pipe 9 of the pump cartridge 3 is thus in fluid communication with the reservoirs 13 and 14 of the microfluidic chip 2, and the whole microfluidic circuit is hermetically closed.
  • the assembled disposable flow chip device of the invention is ready for activation of the fluids to detect and/or quantify one or more analyte(s) present in a biological sample.
  • the pipe 9 of the pump cartridge 3 is brought into contact with one or more roller(s) 27 of the external peristaltic pump 4 in the analyzer.
  • the sample reagent is pumped into the micro-channel 18 and is processed by continuous dynamic flow until it is detected in the detection zone 23 of the microfluidic chip 2.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP15162040.8A 2015-03-31 2015-03-31 Einweg-fluss-chip-vorrichtung Active EP3075451B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP15162040.8A EP3075451B1 (de) 2015-03-31 2015-03-31 Einweg-fluss-chip-vorrichtung

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EP15162040.8A EP3075451B1 (de) 2015-03-31 2015-03-31 Einweg-fluss-chip-vorrichtung

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EP3075451A1 true EP3075451A1 (de) 2016-10-05
EP3075451B1 EP3075451B1 (de) 2020-05-13

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107939634A (zh) * 2017-12-20 2018-04-20 爱科赛智能科技（台州）有限公司 一种无源加热式微型气动泵
WO2020008009A1 (fr) 2018-07-04 2020-01-09 Coris Bioconcept Procede et dispositif pour la detection et differenciation de mutations ponctuelles chez des microorganismes par oligochromatographie
CN112041070A (zh) * 2018-05-15 2020-12-04 宝洁公司 微流体料盒和包括该微流体料盒的微流体递送装置
WO2022062905A1 (zh) * 2020-09-27 2022-03-31 青岛海尔电冰箱有限公司 用于冰箱的微流控检测系统及冰箱

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2672301A1 (fr) * 1991-02-01 1992-08-07 Larzul Daniel Procede et dispositif pour amplifier le nombre d'une sequence definie d'acide nucleique dans un echantillon biologique.
US5632876A (en) 1995-06-06 1997-05-27 David Sarnoff Research Center, Inc. Apparatus and methods for controlling fluid flow in microchannels
US5705018A (en) 1995-12-13 1998-01-06 Hartley; Frank T. Micromachined peristaltic pump
US20040101444A1 (en) * 2002-07-15 2004-05-27 Xeotron Corporation Apparatus and method for fluid delivery to a hybridization station
EP2290354A1 (de) * 2009-08-25 2011-03-02 Hach Lange GmbH Prozess-Analysegerät
WO2014005969A1 (fr) 2012-07-04 2014-01-09 Coris Bioconcept Sprl Procede et dispositif pour la detection rapide de sequences nucleotidiques amplifiees

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2672301A1 (fr) * 1991-02-01 1992-08-07 Larzul Daniel Procede et dispositif pour amplifier le nombre d'une sequence definie d'acide nucleique dans un echantillon biologique.
US5632876A (en) 1995-06-06 1997-05-27 David Sarnoff Research Center, Inc. Apparatus and methods for controlling fluid flow in microchannels
US5705018A (en) 1995-12-13 1998-01-06 Hartley; Frank T. Micromachined peristaltic pump
US20040101444A1 (en) * 2002-07-15 2004-05-27 Xeotron Corporation Apparatus and method for fluid delivery to a hybridization station
EP2290354A1 (de) * 2009-08-25 2011-03-02 Hach Lange GmbH Prozess-Analysegerät
WO2014005969A1 (fr) 2012-07-04 2014-01-09 Coris Bioconcept Sprl Procede et dispositif pour la detection rapide de sequences nucleotidiques amplifiees

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107939634A (zh) * 2017-12-20 2018-04-20 爱科赛智能科技（台州）有限公司 一种无源加热式微型气动泵
CN112041070A (zh) * 2018-05-15 2020-12-04 宝洁公司 微流体料盒和包括该微流体料盒的微流体递送装置
CN112041070B (zh) * 2018-05-15 2022-09-23 宝洁公司 微流体料盒和包括该微流体料盒的微流体递送装置
WO2020008009A1 (fr) 2018-07-04 2020-01-09 Coris Bioconcept Procede et dispositif pour la detection et differenciation de mutations ponctuelles chez des microorganismes par oligochromatographie
BE1026457A1 (fr) 2018-07-04 2020-01-30 Coris Bioconcept Procédé et dispositif pour la détection et différenciation de mutations ponctuelles chez des microorganismes par oligochromatographie
WO2022062905A1 (zh) * 2020-09-27 2022-03-31 青岛海尔电冰箱有限公司 用于冰箱的微流控检测系统及冰箱

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