EP3058332A1 - Procédés et systèmes pour le génotypage d'échantillons génétiques - Google Patents
Procédés et systèmes pour le génotypage d'échantillons génétiquesInfo
- Publication number
- EP3058332A1 EP3058332A1 EP14854801.9A EP14854801A EP3058332A1 EP 3058332 A1 EP3058332 A1 EP 3058332A1 EP 14854801 A EP14854801 A EP 14854801A EP 3058332 A1 EP3058332 A1 EP 3058332A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- sequence
- construct
- reads
- reference sequence
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 132
- 230000002068 genetic effect Effects 0.000 title claims description 30
- 238000003205 genotyping method Methods 0.000 title claims description 15
- 108700028369 Alleles Proteins 0.000 claims description 52
- 238000012163 sequencing technique Methods 0.000 claims description 51
- 230000015654 memory Effects 0.000 claims description 45
- 150000007523 nucleic acids Chemical class 0.000 claims description 41
- 102000039446 nucleic acids Human genes 0.000 claims description 34
- 108020004707 nucleic acids Proteins 0.000 claims description 34
- 239000002773 nucleotide Substances 0.000 claims description 25
- 125000003729 nucleotide group Chemical group 0.000 claims description 24
- 238000003780 insertion Methods 0.000 claims description 23
- 230000037431 insertion Effects 0.000 claims description 23
- 238000012217 deletion Methods 0.000 claims description 16
- 230000037430 deletion Effects 0.000 claims description 16
- 201000010099 disease Diseases 0.000 claims description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 9
- 238000012175 pyrosequencing Methods 0.000 claims description 8
- 210000000349 chromosome Anatomy 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 210000004369 blood Anatomy 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 5
- 238000003786 synthesis reaction Methods 0.000 claims description 5
- 210000001519 tissue Anatomy 0.000 claims description 5
- 210000003780 hair follicle Anatomy 0.000 claims description 2
- 210000002445 nipple Anatomy 0.000 claims description 2
- 210000003296 saliva Anatomy 0.000 claims description 2
- 210000002700 urine Anatomy 0.000 claims description 2
- 206010036790 Productive cough Diseases 0.000 claims 1
- 238000007480 sanger sequencing Methods 0.000 claims 1
- 239000004065 semiconductor Substances 0.000 claims 1
- 238000007841 sequencing by ligation Methods 0.000 claims 1
- 210000003802 sputum Anatomy 0.000 claims 1
- 208000024794 sputum Diseases 0.000 claims 1
- 210000004243 sweat Anatomy 0.000 claims 1
- 230000035772 mutation Effects 0.000 abstract description 27
- 238000004422 calculation algorithm Methods 0.000 description 48
- 108020004414 DNA Proteins 0.000 description 43
- 239000000523 sample Substances 0.000 description 36
- 239000012634 fragment Substances 0.000 description 27
- 238000012545 processing Methods 0.000 description 25
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 21
- 210000004027 cell Anatomy 0.000 description 19
- 230000000875 corresponding effect Effects 0.000 description 18
- 238000004891 communication Methods 0.000 description 16
- 238000004458 analytical method Methods 0.000 description 15
- 239000011324 bead Substances 0.000 description 15
- 230000008569 process Effects 0.000 description 14
- 238000001514 detection method Methods 0.000 description 12
- 238000005516 engineering process Methods 0.000 description 12
- 238000004949 mass spectrometry Methods 0.000 description 12
- 238000013459 approach Methods 0.000 description 11
- 230000014509 gene expression Effects 0.000 description 11
- 238000003752 polymerase chain reaction Methods 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 230000037361 pathway Effects 0.000 description 10
- 102000053602 DNA Human genes 0.000 description 9
- 150000001413 amino acids Chemical group 0.000 description 9
- 239000011159 matrix material Substances 0.000 description 9
- 239000002299 complementary DNA Substances 0.000 description 8
- 238000003860 storage Methods 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- -1 greater than 10 Chemical class 0.000 description 7
- 238000009396 hybridization Methods 0.000 description 7
- 238000010348 incorporation Methods 0.000 description 7
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 238000003491 array Methods 0.000 description 6
- 239000012472 biological sample Substances 0.000 description 6
- 238000004364 calculation method Methods 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 238000004811 liquid chromatography Methods 0.000 description 6
- 238000002493 microarray Methods 0.000 description 6
- 238000007481 next generation sequencing Methods 0.000 description 6
- 238000002864 sequence alignment Methods 0.000 description 6
- 238000006467 substitution reaction Methods 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 125000002015 acyclic group Chemical group 0.000 description 5
- 230000003321 amplification Effects 0.000 description 5
- 230000007614 genetic variation Effects 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 239000013598 vector Substances 0.000 description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- 239000004677 Nylon Substances 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 230000002596 correlated effect Effects 0.000 description 4
- 238000000132 electrospray ionisation Methods 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 229920001778 nylon Polymers 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000003196 serial analysis of gene expression Methods 0.000 description 4
- 230000001360 synchronised effect Effects 0.000 description 4
- 238000012176 true single molecule sequencing Methods 0.000 description 4
- 206010006187 Breast cancer Diseases 0.000 description 3
- 208000026310 Breast neoplasm Diseases 0.000 description 3
- 238000001712 DNA sequencing Methods 0.000 description 3
- 238000002123 RNA extraction Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000090 biomarker Substances 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000002509 fluorescent in situ hybridization Methods 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 238000010802 RNA extraction kit Methods 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- 230000004931 aggregating effect Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000003795 desorption Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000011223 gene expression profiling Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000002865 local sequence alignment Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000005257 nucleotidylation Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 102000054765 polymorphisms of proteins Human genes 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000005945 translocation Effects 0.000 description 2
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
- 206010061818 Disease progression Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 238000003559 RNA-seq method Methods 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 102000004523 Sulfate Adenylyltransferase Human genes 0.000 description 1
- 108010022348 Sulfate adenylyltransferase Proteins 0.000 description 1
- 208000003721 Triple Negative Breast Neoplasms Diseases 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- IRLPACMLTUPBCL-FCIPNVEPSA-N adenosine-5'-phosphosulfate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@@H](CO[P@](O)(=O)OS(O)(=O)=O)[C@H](O)[C@H]1O IRLPACMLTUPBCL-FCIPNVEPSA-N 0.000 description 1
- 150000003838 adenosines Chemical class 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N biotin Natural products N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 230000008711 chromosomal rearrangement Effects 0.000 description 1
- 210000003040 circulating cell Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000030609 dephosphorylation Effects 0.000 description 1
- 238000006209 dephosphorylation reaction Methods 0.000 description 1
- 238000007847 digital PCR Methods 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 230000005750 disease progression Effects 0.000 description 1
- 208000022602 disease susceptibility Diseases 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 238000000105 evaporative light scattering detection Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- LIYGYAHYXQDGEP-UHFFFAOYSA-N firefly oxyluciferin Natural products Oc1csc(n1)-c1nc2ccc(O)cc2s1 LIYGYAHYXQDGEP-UHFFFAOYSA-N 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000007672 fourth generation sequencing Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 235000019689 luncheon sausage Nutrition 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000007902 molecular cytogenetic technique Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- JJVOROULKOMTKG-UHFFFAOYSA-N oxidized Photinus luciferin Chemical compound S1C2=CC(O)=CC=C2N=C1C1=NC(=O)CS1 JJVOROULKOMTKG-UHFFFAOYSA-N 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 108700022487 rRNA Genes Proteins 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 208000022679 triple-negative breast carcinoma Diseases 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 239000002569 water oil cream Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/10—Sequence alignment; Homology search
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/20—Sequence assembly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
Abstract
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361892662P | 2013-10-18 | 2013-10-18 | |
PCT/US2014/061156 WO2015058093A1 (fr) | 2013-10-18 | 2014-10-17 | Procédés et systèmes pour le génotypage d'échantillons génétiques |
Publications (3)
Publication Number | Publication Date |
---|---|
EP3058332A1 true EP3058332A1 (fr) | 2016-08-24 |
EP3058332A4 EP3058332A4 (fr) | 2017-05-10 |
EP3058332B1 EP3058332B1 (fr) | 2019-08-28 |
Family
ID=52828742
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP14854801.9A Active EP3058332B1 (fr) | 2013-10-18 | 2014-10-17 | Procédés et systèmes pour le génotypage d'échantillons génétiques |
Country Status (9)
Country | Link |
---|---|
US (2) | US10078724B2 (fr) |
EP (1) | EP3058332B1 (fr) |
JP (1) | JP2017500004A (fr) |
KR (1) | KR20160062763A (fr) |
CN (1) | CN105793689B (fr) |
AU (1) | AU2014337089B2 (fr) |
CA (1) | CA2927102C (fr) |
SG (1) | SG11201602903XA (fr) |
WO (1) | WO2015058093A1 (fr) |
Families Citing this family (51)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9898575B2 (en) | 2013-08-21 | 2018-02-20 | Seven Bridges Genomics Inc. | Methods and systems for aligning sequences |
US9116866B2 (en) | 2013-08-21 | 2015-08-25 | Seven Bridges Genomics Inc. | Methods and systems for detecting sequence variants |
JP2016533182A (ja) | 2013-10-18 | 2016-10-27 | セブン ブリッジズ ジェノミクス インコーポレイテッド | 疾患に誘導された変異を同定するための方法およびシステム |
WO2015058095A1 (fr) | 2013-10-18 | 2015-04-23 | Seven Bridges Genomics Inc. | Procédés et systèmes de quantification d'alignement de séquences |
WO2015058093A1 (fr) | 2013-10-18 | 2015-04-23 | Seven Bridges Genomics Inc. | Procédés et systèmes pour le génotypage d'échantillons génétiques |
US11049587B2 (en) | 2013-10-18 | 2021-06-29 | Seven Bridges Genomics Inc. | Methods and systems for aligning sequences in the presence of repeating elements |
US9092402B2 (en) | 2013-10-21 | 2015-07-28 | Seven Bridges Genomics Inc. | Systems and methods for using paired-end data in directed acyclic structure |
WO2015105963A1 (fr) | 2014-01-10 | 2015-07-16 | Seven Bridges Genomics Inc. | Systèmes et procédés d'utilisation d'allèles connus en cartographie de lectures |
US9670530B2 (en) * | 2014-01-30 | 2017-06-06 | Illumina, Inc. | Haplotype resolved genome sequencing |
US9817944B2 (en) | 2014-02-11 | 2017-11-14 | Seven Bridges Genomics Inc. | Systems and methods for analyzing sequence data |
WO2016141294A1 (fr) | 2015-03-05 | 2016-09-09 | Seven Bridges Genomics Inc. | Systèmes et procédés d'analyse de motifs génomiques |
US10395759B2 (en) | 2015-05-18 | 2019-08-27 | Regeneron Pharmaceuticals, Inc. | Methods and systems for copy number variant detection |
US10229519B2 (en) | 2015-05-22 | 2019-03-12 | The University Of British Columbia | Methods for the graphical representation of genomic sequence data |
US20160364523A1 (en) * | 2015-06-11 | 2016-12-15 | Seven Bridges Genomics Inc. | Systems and methods for identifying microorganisms |
US10793895B2 (en) | 2015-08-24 | 2020-10-06 | Seven Bridges Genomics Inc. | Systems and methods for epigenetic analysis |
US10584380B2 (en) | 2015-09-01 | 2020-03-10 | Seven Bridges Genomics Inc. | Systems and methods for mitochondrial analysis |
US10724110B2 (en) | 2015-09-01 | 2020-07-28 | Seven Bridges Genomics Inc. | Systems and methods for analyzing viral nucleic acids |
US11347704B2 (en) | 2015-10-16 | 2022-05-31 | Seven Bridges Genomics Inc. | Biological graph or sequence serialization |
CN108449995B (zh) | 2015-11-06 | 2022-02-01 | 文塔纳医疗系统公司 | 代表性诊断 |
US20170199960A1 (en) | 2016-01-07 | 2017-07-13 | Seven Bridges Genomics Inc. | Systems and methods for adaptive local alignment for graph genomes |
US10364468B2 (en) | 2016-01-13 | 2019-07-30 | Seven Bridges Genomics Inc. | Systems and methods for analyzing circulating tumor DNA |
SG11201805600VA (en) | 2016-01-15 | 2018-07-30 | Ventana Med Syst Inc | Deep sequencing profiling of tumors |
US10460829B2 (en) | 2016-01-26 | 2019-10-29 | Seven Bridges Genomics Inc. | Systems and methods for encoding genetic variation for a population |
JP6648549B2 (ja) * | 2016-02-19 | 2020-02-14 | 富士通株式会社 | 変異情報処理装置、方法及びプログラム |
US10262102B2 (en) * | 2016-02-24 | 2019-04-16 | Seven Bridges Genomics Inc. | Systems and methods for genotyping with graph reference |
US10790044B2 (en) | 2016-05-19 | 2020-09-29 | Seven Bridges Genomics Inc. | Systems and methods for sequence encoding, storage, and compression |
US10600499B2 (en) | 2016-07-13 | 2020-03-24 | Seven Bridges Genomics Inc. | Systems and methods for reconciling variants in sequence data relative to reference sequence data |
US11289177B2 (en) | 2016-08-08 | 2022-03-29 | Seven Bridges Genomics, Inc. | Computer method and system of identifying genomic mutations using graph-based local assembly |
US11250931B2 (en) | 2016-09-01 | 2022-02-15 | Seven Bridges Genomics Inc. | Systems and methods for detecting recombination |
US10319465B2 (en) | 2016-11-16 | 2019-06-11 | Seven Bridges Genomics Inc. | Systems and methods for aligning sequences to graph references |
US11347844B2 (en) | 2017-03-01 | 2022-05-31 | Seven Bridges Genomics, Inc. | Data security in bioinformatic sequence analysis |
US10726110B2 (en) | 2017-03-01 | 2020-07-28 | Seven Bridges Genomics, Inc. | Watermarking for data security in bioinformatic sequence analysis |
WO2018189040A1 (fr) | 2017-04-14 | 2018-10-18 | Ventana Medical Systems, Inc. | Séparation, basée sur la taille, de tissus fixes dissociés |
EP3679575A1 (fr) * | 2017-09-07 | 2020-07-15 | Regeneron Pharmaceuticals, Inc. | Systèmes et procédés d'exploitation de la parenté dans l'analyse de données génomiques |
EP3467690A1 (fr) * | 2017-10-06 | 2019-04-10 | Emweb bvba | Procédé d'alignement amélioré pour séquences d'acide nucléique |
JP7054133B2 (ja) * | 2017-11-09 | 2022-04-13 | 国立研究開発法人国立がん研究センター | 配列解析方法、配列解析装置、参照配列の生成方法、参照配列生成装置、プログラム、および記録媒体 |
CN111656179B (zh) | 2017-11-13 | 2023-11-03 | 豪夫迈·罗氏有限公司 | 用于使用表位电泳进行样品分析的装置 |
US20210382002A1 (en) | 2018-10-12 | 2021-12-09 | Roche Sequencing Solutions, Inc. | Detection methods for epitachophoresis workflow automation |
WO2020112566A1 (fr) | 2018-11-29 | 2020-06-04 | Ventana Medical Systems, Inc. | Surveillance de pathologie liée à l'adn tumoral circulant personnalisée par séquençage d'adn représentatif |
CN113228190A (zh) | 2018-12-23 | 2021-08-06 | 豪夫迈·罗氏有限公司 | 基于预测的肿瘤突变负荷的肿瘤分类 |
US11848073B2 (en) * | 2019-04-03 | 2023-12-19 | University Of Central Florida Research Foundation, Inc. | Methods and system for efficient indexing for genetic genealogical discovery in large genotype databases |
CN110033829B (zh) * | 2019-04-11 | 2021-07-23 | 北京诺禾心康基因科技有限公司 | 基于差异snp标记物的同源基因的融合检测方法 |
CN110060737B (zh) * | 2019-04-30 | 2023-04-18 | 上海诚明融鑫科技有限公司 | 一种基于最大频率虚拟个体的str快速比对方法及系统 |
EP3969583A1 (fr) | 2019-05-14 | 2022-03-23 | F. Hoffmann-La Roche AG | Dispositifs et procédés d'analyse d'échantillons |
WO2020264260A1 (fr) * | 2019-06-27 | 2020-12-30 | Zymergen Inc. | Système d'automatisation de laboratoire mettant en œuvre un trajet efficace pour des transferts de matériau et de matériel de laboratoire |
EP4093543A2 (fr) | 2020-01-22 | 2022-11-30 | F. Hoffmann-La Roche AG | Dispositifs microfluidiques de piégeage de billes et procédés de préparation de banque de séquences de nouvelle génération |
EP4162083A1 (fr) | 2020-06-08 | 2023-04-12 | F. Hoffmann-La Roche AG | Procédés et compositions de détection de réagencements structuraux dans un génome |
WO2022008578A1 (fr) | 2020-07-08 | 2022-01-13 | F. Hoffmann-La Roche Ag | Déplétion ciblée de molécules de bibliothèque non cibles à l'aide d'amorces poison pendant la capture cible de bibliothèques de séquençage de nouvelle génération |
EP4228793A1 (fr) | 2020-10-15 | 2023-08-23 | Kapa Biosystems, Inc. | Dispositifs électrophorétiques et procédés de préparation de bibliothèque de séquençage de nouvelle génération |
WO2022194764A1 (fr) | 2021-03-15 | 2022-09-22 | F. Hoffmann-La Roche Ag | Séquençage ciblé de nouvelle génération par l'intermédiaire d'une extension d'amorce ancrée |
WO2022200485A1 (fr) | 2021-03-26 | 2022-09-29 | F. Hoffmann-La Roche Ag | Formulations de tampons d'hybridation |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011139797A2 (fr) * | 2010-04-27 | 2011-11-10 | Spiral Genetics Inc. | Procédé et système d'analyse et de correction d'erreurs de séquences biologiques et d'inférence de relations pour des échantillons multiples |
US8209130B1 (en) * | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
Family Cites Families (136)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5242794A (en) | 1984-12-13 | 1993-09-07 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5583024A (en) | 1985-12-02 | 1996-12-10 | The Regents Of The University Of California | Recombinant expression of Coleoptera luciferase |
US4988617A (en) | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
US5511158A (en) | 1994-08-04 | 1996-04-23 | Thinking Machines Corporation | System and method for creating and evolving directed graphs |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
US5701256A (en) | 1995-05-31 | 1997-12-23 | Cold Spring Harbor Laboratory | Method and apparatus for biological sequence comparison |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
US6054278A (en) | 1997-05-05 | 2000-04-25 | The Perkin-Elmer Corporation | Ribosomal RNA gene polymorphism based microorganism identification |
EP1027145A4 (fr) | 1997-09-17 | 2004-08-25 | Gentra Systems Inc | Appareil et procedes permettant d'isoler un acide nucleique |
US6054276A (en) | 1998-02-23 | 2000-04-25 | Macevicz; Stephen C. | DNA restriction site mapping |
US6223128B1 (en) | 1998-06-29 | 2001-04-24 | Dnstar, Inc. | DNA sequence assembly system |
US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
GB9901475D0 (en) | 1999-01-22 | 1999-03-17 | Pyrosequencing Ab | A method of DNA sequencing |
US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
EP1218543A2 (fr) | 1999-09-29 | 2002-07-03 | Solexa Ltd. | Sequen age de polynucleotides |
US6582938B1 (en) | 2001-05-11 | 2003-06-24 | Affymetrix, Inc. | Amplification of nucleic acids |
US6448717B1 (en) | 2000-07-17 | 2002-09-10 | Micron Technology, Inc. | Method and apparatuses for providing uniform electron beams from field emission displays |
US6925389B2 (en) | 2000-07-18 | 2005-08-02 | Correlogic Systems, Inc., | Process for discriminating between biological states based on hidden patterns from biological data |
NO20004869D0 (no) | 2000-09-28 | 2000-09-28 | Torbjoern Rognes | Metode for hurtig optimal lokal sekvensjustering ved bruk av parallell prosessering |
EP1368497A4 (fr) | 2001-03-12 | 2007-08-15 | California Inst Of Techn | Procedes et appareil d'analyse de sequences de polynucleotide par extension de base asynchrone |
US6890763B2 (en) | 2001-04-30 | 2005-05-10 | Syn X Pharma, Inc. | Biopolymer marker indicative of disease state having a molecular weight of 1350 daltons |
US7809509B2 (en) | 2001-05-08 | 2010-10-05 | Ip Genesis, Inc. | Comparative mapping and assembly of nucleic acid sequences |
US7577554B2 (en) | 2001-07-03 | 2009-08-18 | I2 Technologies Us, Inc. | Workflow modeling using an acyclic directed graph data structure |
US20040023209A1 (en) | 2001-11-28 | 2004-02-05 | Jon Jonasson | Method for identifying microorganisms based on sequencing gene fragments |
US6989100B2 (en) | 2002-05-09 | 2006-01-24 | Ppd Biomarker Discovery Sciences, Llc | Methods for time-alignment of liquid chromatography-mass spectrometry data |
US7321623B2 (en) | 2002-10-01 | 2008-01-22 | Avocent Corporation | Video compression system |
US7225324B2 (en) | 2002-10-31 | 2007-05-29 | Src Computers, Inc. | Multi-adaptive processing systems and techniques for enhancing parallelism and performance of computational functions |
WO2004061616A2 (fr) | 2002-12-27 | 2004-07-22 | Rosetta Inpharmatics Llc | Systemes et procedes informatiques permettant d'associer des genes avec des caracteristiques au moyen de donnees heterospecifiques |
US7885840B2 (en) | 2003-01-07 | 2011-02-08 | Sap Aktiengesellschaft | System and method of flexible workflow management |
US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
US7953891B2 (en) | 2003-03-18 | 2011-05-31 | Microsoft Corporation | Systems and methods for scheduling data flow execution based on an arbitrary graph describing the desired data flow |
JP2005092719A (ja) | 2003-09-19 | 2005-04-07 | Nec Corp | ハプロタイプ推定方法、推定装置、プログラム |
EP2202322A1 (fr) | 2003-10-31 | 2010-06-30 | AB Advanced Genetic Analysis Corporation | Procédés de production d'étiquette appariée à partir d'une séquence d'acide nucléique et leurs procédés d'utilisation |
US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US20060195266A1 (en) | 2005-02-25 | 2006-08-31 | Yeatman Timothy J | Methods for predicting cancer outcome and gene signatures for use therein |
WO2005107412A2 (fr) | 2004-04-30 | 2005-11-17 | Rosetta Inpharmatics Llc | Systemes et procedes pour la reconstruction de reseaux geniques dans des populations resultant d'une segregation |
US7642511B2 (en) | 2004-09-30 | 2010-01-05 | Ut-Battelle, Llc | Ultra high mass range mass spectrometer systems |
WO2006052242A1 (fr) | 2004-11-08 | 2006-05-18 | Seirad, Inc. | Procedes et systemes pour comprimer et comparer des donnees genomiques |
US7483585B2 (en) | 2004-12-01 | 2009-01-27 | Ati Technologies Ulc | Image compression using variable bit size run length encoding |
CN101189345A (zh) * | 2005-02-01 | 2008-05-28 | Ab先进基因分析公司 | 珠基测序的试剂、方法和文库 |
EP2003214B1 (fr) * | 2005-02-01 | 2013-04-10 | AB Advanced Genetic Analysis Corporation | Réactifs, procédés, et pharmacothèques pour séquençage de billes |
WO2007145612A1 (fr) | 2005-06-06 | 2007-12-21 | 454 Life Sciences Corporation | Séquençage d'extrémités appariées |
US7329860B2 (en) | 2005-11-23 | 2008-02-12 | Illumina, Inc. | Confocal imaging methods and apparatus |
KR100722504B1 (ko) | 2006-01-18 | 2007-05-29 | 학교법인 포항공과대학교 | 비선형 공정 계획 생성 방법 및 이를 이용한 인터넷 기반step-nc 시스템 |
US7580918B2 (en) | 2006-03-03 | 2009-08-25 | Adobe Systems Incorporated | System and method of efficiently representing and searching directed acyclic graph structures in databases |
GB2436564A (en) | 2006-03-31 | 2007-10-03 | Plant Bioscience Ltd | Prediction of heterosis and other traits by transcriptome analysis |
US7282337B1 (en) | 2006-04-14 | 2007-10-16 | Helicos Biosciences Corporation | Methods for increasing accuracy of nucleic acid sequencing |
US7702468B2 (en) | 2006-05-03 | 2010-04-20 | Population Diagnostics, Inc. | Evaluating genetic disorders |
US7754429B2 (en) | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
EP2463389A1 (fr) | 2006-10-20 | 2012-06-13 | Innogenetics N.V. | Méthode d'analyse des variations de séquence dans la zone génomique NS5B du VHC |
CA2672315A1 (fr) | 2006-12-14 | 2008-06-26 | Ion Torrent Systems Incorporated | Procedes et appareil permettant de mesurer des analytes en utilisant des matrices de tec a grande echelle |
US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
WO2008092150A1 (fr) | 2007-01-26 | 2008-07-31 | Illumina, Inc. | Système et procédé de séquençage d'acides nucléiques |
WO2008098014A2 (fr) | 2007-02-05 | 2008-08-14 | Applied Biosystems, Llc | Système et procédé pour identification d'insertion-délétion en utilisant un séquençage à lecture courte |
US8146099B2 (en) | 2007-09-27 | 2012-03-27 | Microsoft Corporation | Service-oriented pipeline based architecture |
US20090119313A1 (en) | 2007-11-02 | 2009-05-07 | Ioactive Inc. | Determining structure of binary data using alignment algorithms |
US8478544B2 (en) | 2007-11-21 | 2013-07-02 | Cosmosid Inc. | Direct identification and measurement of relative populations of microorganisms with direct DNA sequencing and probabilistic methods |
US20090233809A1 (en) | 2008-03-04 | 2009-09-17 | Affymetrix, Inc. | Resequencing methods for identification of sequence variants |
US8271206B2 (en) | 2008-04-21 | 2012-09-18 | Softgenetics Llc | DNA sequence assembly methods of short reads |
US20100010992A1 (en) | 2008-07-10 | 2010-01-14 | Morris Robert P | Methods And Systems For Resolving A Location Information To A Network Identifier |
KR100992169B1 (ko) | 2008-07-25 | 2010-11-04 | 한국생명공학연구원 | 정보 분석프로세스 추천 설계시스템 및 방법 |
US20100035252A1 (en) | 2008-08-08 | 2010-02-11 | Ion Torrent Systems Incorporated | Methods for sequencing individual nucleic acids under tension |
US9347089B2 (en) | 2008-09-19 | 2016-05-24 | Children's Medical Center Corporation | Therapeutic and diagnostic strategies |
US8546128B2 (en) | 2008-10-22 | 2013-10-01 | Life Technologies Corporation | Fluidics system for sequential delivery of reagents |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US8691510B2 (en) | 2008-11-07 | 2014-04-08 | Sequenta, Inc. | Sequence analysis of complex amplicons |
US8370079B2 (en) | 2008-11-20 | 2013-02-05 | Pacific Biosciences Of California, Inc. | Algorithms for sequence determination |
WO2010075570A2 (fr) | 2008-12-24 | 2010-07-01 | New York University | Procédés, support accessible par ordinateur et systèmes d'assemblage piloté par un score de séquences dispersées de génome entier |
EP2394152B1 (fr) | 2009-02-03 | 2019-05-01 | ANDE Corporation | Purification d'acide nucléique |
US8352195B2 (en) | 2009-03-20 | 2013-01-08 | Siemens Corporation | Methods and systems for identifying PCR primers specific to one or more target genomes |
WO2010127045A2 (fr) | 2009-04-29 | 2010-11-04 | Complete Genomics, Inc. | Procédé et système pour appeler des variations dans une séquence polynucléotidique d'échantillon par rapport à une séquence polynucléotidique de référence |
US8574835B2 (en) | 2009-05-29 | 2013-11-05 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
US8673627B2 (en) | 2009-05-29 | 2014-03-18 | Life Technologies Corporation | Apparatus and methods for performing electrochemical reactions |
US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
WO2011050341A1 (fr) | 2009-10-22 | 2011-04-28 | National Center For Genome Resources | Méthodes et systèmes pour l'analyse de séquençage médical |
AU2010341829A1 (en) | 2010-01-14 | 2012-08-02 | Fluor Technologies Corporation | 3D plant modeling systems and methods |
EP2536854B1 (fr) | 2010-02-18 | 2017-07-19 | The Johns Hopkins University | Biomarqueurs tumoraux personnalisés |
US9165109B2 (en) | 2010-02-24 | 2015-10-20 | Pacific Biosciences Of California, Inc. | Sequence assembly and consensus sequence determination |
US20110257889A1 (en) | 2010-02-24 | 2011-10-20 | Pacific Biosciences Of California, Inc. | Sequence assembly and consensus sequence determination |
US20120030566A1 (en) | 2010-07-28 | 2012-02-02 | Victor B Michael | System with touch-based selection of data items |
EP3822975A1 (fr) | 2010-09-09 | 2021-05-19 | Fabric Genomics, Inc. | Annotation, analyse et outil de sélection de variants |
US9309556B2 (en) | 2010-09-24 | 2016-04-12 | The Board Of Trustees Of The Leland Stanford Junior University | Direct capture, amplification and sequencing of target DNA using immobilized primers |
EP2663655B1 (fr) | 2011-01-14 | 2015-09-02 | Keygene N.V. | Génotypage fondé sur des séquences aléatoires à extrémités appariées |
BR112013018139A8 (pt) | 2011-01-19 | 2018-02-06 | Koninklijke Philips Electronics Nv | Método para processar dados genômicos de um indivíduo, uso de informação de sequência genômica, opcionalmente na combinação com informação de expressão de gene, apoio à decisão clínica e sistema de armazenamento e sistema |
US20120239706A1 (en) | 2011-03-18 | 2012-09-20 | Los Alamos National Security, Llc | Computer-facilitated parallel information alignment and analysis |
WO2012142531A2 (fr) | 2011-04-14 | 2012-10-18 | Complete Genomics, Inc. | Traitement et analyse de données de séquences d'acides nucléiques complexes |
US9506167B2 (en) | 2011-07-29 | 2016-11-29 | Ginkgo Bioworks, Inc. | Methods and systems for cell state quantification |
WO2013035904A1 (fr) | 2011-09-08 | 2013-03-14 | 한국과학기술정보연구원 | Système et procédé de traitement de pipeline d'analyse d'informations biométriques |
KR101282798B1 (ko) | 2011-09-08 | 2013-07-04 | 한국과학기술정보연구원 | 생명 정보 분석 파이프라인 처리 시스템 및 방법 |
US20130073214A1 (en) * | 2011-09-20 | 2013-03-21 | Life Technologies Corporation | Systems and methods for identifying sequence variation |
EP2608096B1 (fr) | 2011-12-24 | 2020-08-05 | Tata Consultancy Services Ltd. | Compression de fichiers de données génomiques |
WO2013097257A1 (fr) | 2011-12-31 | 2013-07-04 | 深圳华大基因科技有限公司 | Procédé et système d'analyse de gène de fusion |
WO2013106737A1 (fr) | 2012-01-13 | 2013-07-18 | Data2Bio | Génotypage par séquençage de nouvelle génération |
US9047133B2 (en) | 2012-03-02 | 2015-06-02 | Vmware, Inc. | Single, logical, multi-tier application blueprint used for deployment and management of multiple physical applications in a cloud environment |
US9552458B2 (en) | 2012-03-16 | 2017-01-24 | The Research Institute At Nationwide Children's Hospital | Comprehensive analysis pipeline for discovery of human genetic variation |
US20130345066A1 (en) | 2012-05-09 | 2013-12-26 | Life Technologies Corporation | Systems and methods for identifying sequence variation |
US20130324417A1 (en) | 2012-06-04 | 2013-12-05 | Good Start Genetics, Inc. | Determining the clinical significance of variant sequences |
US9104656B2 (en) | 2012-07-03 | 2015-08-11 | International Business Machines Corporation | Using lexical analysis and parsing in genome research |
US10777301B2 (en) | 2012-07-13 | 2020-09-15 | Pacific Biosciences For California, Inc. | Hierarchical genome assembly method using single long insert library |
CN104756445A (zh) | 2012-11-06 | 2015-07-01 | 惠普发展公司,有限责任合伙企业 | 增强的图遍历 |
US20140129201A1 (en) | 2012-11-07 | 2014-05-08 | Good Start Genetics, Inc. | Validation of genetic tests |
WO2014113204A1 (fr) | 2013-01-17 | 2014-07-24 | Personalis, Inc. | Procédés et systèmes d'analyse génétique |
US10346551B2 (en) | 2013-01-24 | 2019-07-09 | New York University | Systems, methods and computer-accessible mediums for utilizing pattern matching in stringomes |
US10032195B2 (en) | 2013-03-13 | 2018-07-24 | Airline Tariff Publishing Company | System, method and computer program product for providing a fare analytic engine |
US9087007B2 (en) | 2013-03-14 | 2015-07-21 | International Business Machines Corporation | Generating fault tolerant connectivity API |
US9477779B2 (en) | 2013-03-15 | 2016-10-25 | Neo Technology, Inc. | Graph database devices and methods for partitioning graphs |
US9189224B2 (en) | 2013-07-11 | 2015-11-17 | Oracle International Corporation | Forming an upgrade recommendation in a cloud computing environment |
US9898575B2 (en) | 2013-08-21 | 2018-02-20 | Seven Bridges Genomics Inc. | Methods and systems for aligning sequences |
US9116866B2 (en) | 2013-08-21 | 2015-08-25 | Seven Bridges Genomics Inc. | Methods and systems for detecting sequence variants |
US20150066383A1 (en) | 2013-09-03 | 2015-03-05 | Seven Bridges Genomics Inc. | Collapsible modular genomic pipeline |
EP3053073B1 (fr) | 2013-09-30 | 2019-07-03 | Seven Bridges Genomics Inc. | Procédés et système de détection de variantes de séquences |
US9953130B2 (en) | 2013-10-01 | 2018-04-24 | Life Technologies Corporation | Systems and methods for detecting structural variants |
JP6491651B2 (ja) | 2013-10-15 | 2019-03-27 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. | 高解像度での対立遺伝子の同定 |
JP2016533182A (ja) | 2013-10-18 | 2016-10-27 | セブン ブリッジズ ジェノミクス インコーポレイテッド | 疾患に誘導された変異を同定するための方法およびシステム |
US11049587B2 (en) | 2013-10-18 | 2021-06-29 | Seven Bridges Genomics Inc. | Methods and systems for aligning sequences in the presence of repeating elements |
WO2015058095A1 (fr) | 2013-10-18 | 2015-04-23 | Seven Bridges Genomics Inc. | Procédés et systèmes de quantification d'alignement de séquences |
WO2015058093A1 (fr) | 2013-10-18 | 2015-04-23 | Seven Bridges Genomics Inc. | Procédés et systèmes pour le génotypage d'échantillons génétiques |
US9092402B2 (en) | 2013-10-21 | 2015-07-28 | Seven Bridges Genomics Inc. | Systems and methods for using paired-end data in directed acyclic structure |
WO2015105963A1 (fr) | 2014-01-10 | 2015-07-16 | Seven Bridges Genomics Inc. | Systèmes et procédés d'utilisation d'allèles connus en cartographie de lectures |
US9817944B2 (en) | 2014-02-11 | 2017-11-14 | Seven Bridges Genomics Inc. | Systems and methods for analyzing sequence data |
WO2016141294A1 (fr) | 2015-03-05 | 2016-09-09 | Seven Bridges Genomics Inc. | Systèmes et procédés d'analyse de motifs génomiques |
US20160364523A1 (en) | 2015-06-11 | 2016-12-15 | Seven Bridges Genomics Inc. | Systems and methods for identifying microorganisms |
US10793895B2 (en) | 2015-08-24 | 2020-10-06 | Seven Bridges Genomics Inc. | Systems and methods for epigenetic analysis |
US10584380B2 (en) | 2015-09-01 | 2020-03-10 | Seven Bridges Genomics Inc. | Systems and methods for mitochondrial analysis |
US10724110B2 (en) | 2015-09-01 | 2020-07-28 | Seven Bridges Genomics Inc. | Systems and methods for analyzing viral nucleic acids |
US11347704B2 (en) | 2015-10-16 | 2022-05-31 | Seven Bridges Genomics Inc. | Biological graph or sequence serialization |
US20170193351A1 (en) | 2015-12-30 | 2017-07-06 | Micron Technology, Inc. | Methods and systems for vector length management |
US20170199960A1 (en) | 2016-01-07 | 2017-07-13 | Seven Bridges Genomics Inc. | Systems and methods for adaptive local alignment for graph genomes |
US20170199959A1 (en) | 2016-01-13 | 2017-07-13 | Seven Bridges Genomics Inc. | Genetic analysis systems and methods |
US10364468B2 (en) | 2016-01-13 | 2019-07-30 | Seven Bridges Genomics Inc. | Systems and methods for analyzing circulating tumor DNA |
US10262102B2 (en) | 2016-02-24 | 2019-04-16 | Seven Bridges Genomics Inc. | Systems and methods for genotyping with graph reference |
-
2014
- 2014-10-17 WO PCT/US2014/061156 patent/WO2015058093A1/fr active Application Filing
- 2014-10-17 CN CN201480066185.4A patent/CN105793689B/zh active Active
- 2014-10-17 CA CA2927102A patent/CA2927102C/fr active Active
- 2014-10-17 EP EP14854801.9A patent/EP3058332B1/fr active Active
- 2014-10-17 AU AU2014337089A patent/AU2014337089B2/en active Active
- 2014-10-17 US US14/517,406 patent/US10078724B2/en active Active
- 2014-10-17 JP JP2016523194A patent/JP2017500004A/ja active Pending
- 2014-10-17 KR KR1020167012613A patent/KR20160062763A/ko not_active Application Discontinuation
- 2014-10-17 SG SG11201602903XA patent/SG11201602903XA/en unknown
-
2018
- 2018-09-18 US US16/134,619 patent/US20190272891A1/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011139797A2 (fr) * | 2010-04-27 | 2011-11-10 | Spiral Genetics Inc. | Procédé et système d'analyse et de correction d'erreurs de séquences biologiques et d'inférence de relations pour des échantillons multiples |
US8209130B1 (en) * | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
Non-Patent Citations (2)
Title |
---|
CHIN CHEN-SHAN ET AL: "Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data.", NATURE METHODS, vol. 10, no. 6, June 2013 (2013-06-01), XP002768382, ISSN: 1548-7105, DOI: 10.1038/NMETH.2474 * |
See also references of WO2015058093A1 * |
Also Published As
Publication number | Publication date |
---|---|
AU2014337089A1 (en) | 2016-05-05 |
WO2015058093A1 (fr) | 2015-04-23 |
CA2927102C (fr) | 2022-08-30 |
EP3058332A4 (fr) | 2017-05-10 |
US20190272891A1 (en) | 2019-09-05 |
KR20160062763A (ko) | 2016-06-02 |
SG11201602903XA (en) | 2016-05-30 |
CA2927102A1 (fr) | 2015-04-23 |
US20150199472A1 (en) | 2015-07-16 |
CN105793689B (zh) | 2020-04-17 |
CN105793689A (zh) | 2016-07-20 |
JP2017500004A (ja) | 2017-01-05 |
US10078724B2 (en) | 2018-09-18 |
EP3058332B1 (fr) | 2019-08-28 |
AU2014337089B2 (en) | 2019-08-08 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11837328B2 (en) | Methods and systems for detecting sequence variants | |
US20210280272A1 (en) | Methods and systems for quantifying sequence alignment | |
US11211146B2 (en) | Methods and systems for aligning sequences | |
US20210398616A1 (en) | Methods and systems for aligning sequences in the presence of repeating elements | |
US20190272891A1 (en) | Methods and systems for genotyping genetic samples | |
AU2014324438B2 (en) | Methods and system for detecting sequence variants | |
AU2014337093B2 (en) | Methods and systems for identifying disease-induced mutations |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20160427 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
AX | Request for extension of the european patent |
Extension state: BA ME |
|
DAX | Request for extension of the european patent (deleted) | ||
RIC1 | Information provided on ipc code assigned before grant |
Ipc: G01N 1/00 20060101AFI20170322BHEP |
|
A4 | Supplementary search report drawn up and despatched |
Effective date: 20170412 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: G01N 1/00 20060101AFI20170405BHEP |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
|
17Q | First examination report despatched |
Effective date: 20171204 |
|
RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: SEVEN BRIDGES GENOMICS INC. |
|
REG | Reference to a national code |
Ref country code: DE Ref legal event code: R079 Ref document number: 602014052714 Country of ref document: DE Free format text: PREVIOUS MAIN CLASS: G01N0001000000 Ipc: G16B0030100000 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: G16B 30/10 20190101AFI20190327BHEP Ipc: C12Q 1/6869 20180101ALN20190327BHEP |
|
GRAP | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOSNIGR1 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: GRANT OF PATENT IS INTENDED |
|
INTG | Intention to grant announced |
Effective date: 20190509 |
|
GRAS | Grant fee paid |
Free format text: ORIGINAL CODE: EPIDOSNIGR3 |
|
GRAA | (expected) grant |
Free format text: ORIGINAL CODE: 0009210 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE PATENT HAS BEEN GRANTED |
|
AK | Designated contracting states |
Kind code of ref document: B1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
REG | Reference to a national code |
Ref country code: GB Ref legal event code: FG4D |
|
REG | Reference to a national code |
Ref country code: CH Ref legal event code: EP |
|
REG | Reference to a national code |
Ref country code: DE Ref legal event code: R096 Ref document number: 602014052714 Country of ref document: DE |
|
REG | Reference to a national code |
Ref country code: AT Ref legal event code: REF Ref document number: 1173384 Country of ref document: AT Kind code of ref document: T Effective date: 20190915 |
|
REG | Reference to a national code |
Ref country code: IE Ref legal event code: FG4D |
|
REG | Reference to a national code |
Ref country code: NL Ref legal event code: MP Effective date: 20190828 |
|
REG | Reference to a national code |
Ref country code: LT Ref legal event code: MG4D |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: FI Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: NO Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20191128 Ref country code: PT Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20191230 Ref country code: BG Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20191128 Ref country code: SE Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: NL Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: HR Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: LT Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: IS Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20191228 Ref country code: LV Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: GR Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20191129 Ref country code: RS Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: AL Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: ES Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 |
|
REG | Reference to a national code |
Ref country code: AT Ref legal event code: MK05 Ref document number: 1173384 Country of ref document: AT Kind code of ref document: T Effective date: 20190828 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: TR Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: EE Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: PL Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: DK Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: RO Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: IT Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: AT Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: SM Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: CZ Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: MC Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: IS Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20200224 Ref country code: SK Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 |
|
REG | Reference to a national code |
Ref country code: DE Ref legal event code: R097 Ref document number: 602014052714 Country of ref document: DE |
|
PLBE | No opposition filed within time limit |
Free format text: ORIGINAL CODE: 0009261 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT |
|
PG2D | Information on lapse in contracting state deleted |
Ref country code: IS |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: LU Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20191017 |
|
26N | No opposition filed |
Effective date: 20200603 |
|
REG | Reference to a national code |
Ref country code: BE Ref legal event code: MM Effective date: 20191031 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: SI Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 Ref country code: BE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20191031 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: IE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20191017 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: CY Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: HU Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO Effective date: 20141017 Ref country code: MT Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: MK Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20190828 |
|
P01 | Opt-out of the competence of the unified patent court (upc) registered |
Effective date: 20230511 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: GB Payment date: 20231027 Year of fee payment: 10 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: FR Payment date: 20231025 Year of fee payment: 10 Ref country code: DE Payment date: 20231027 Year of fee payment: 10 Ref country code: CH Payment date: 20231102 Year of fee payment: 10 |