EP2921221A1 - Mélange haute énergie pour une préparation automatique d'échantillon dans un déroulement d'opérations en série - Google Patents

Mélange haute énergie pour une préparation automatique d'échantillon dans un déroulement d'opérations en série Download PDF

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Publication number
EP2921221A1
EP2921221A1 EP15000816.7A EP15000816A EP2921221A1 EP 2921221 A1 EP2921221 A1 EP 2921221A1 EP 15000816 A EP15000816 A EP 15000816A EP 2921221 A1 EP2921221 A1 EP 2921221A1
Authority
EP
European Patent Office
Prior art keywords
motor
sample
mixing
sample container
mixer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP15000816.7A
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German (de)
English (en)
Inventor
Werner DÖBELIN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP2921221A1 publication Critical patent/EP2921221A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/10—Mixers with shaking, oscillating, or vibrating mechanisms with a mixing receptacle rotating alternately in opposite directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes

Definitions

  • the present invention relates to a high-energy mixer for automatic sample preparation in a serial process sequence within a fully automatic analysis system, in particular in the field of LC-MS analysis.
  • reaction vessels may be, for example, Erlenmeyer flasks or microtiter plates.
  • mixing devices are known for this mixing.
  • a mixing device is a so-called vortex mixer.
  • the reaction vessel in which the solutions or other substances to be mixed are pressed on a shaker attachment in the form of a rubber mold, which is located on the upper base plate of the mixer.
  • the mixing is then carried out by intensive vibration with usual speed ranges of up to 2500 min -1 .
  • Such vortex mixers can be used in continuous or in short-term operation.
  • the problem is that they small or smallest amounts of samples, as they arise in particular in samples from the biological-medical field, such as blood samples, which are located in correspondingly small-sized conical sample containers, not or insufficiently mix ,
  • the samples must first be processed method-specific, in order to then supply them to the actual measuring device.
  • the blood plasma must be diluted, an internal standard added, and a precipitant added.
  • the thus treated sample is then shaken and centrifuged to allow the supernatant to be delivered as a measurable sample solution to the LC-MS.
  • the object of the present invention was to provide a mixer with which an automatic sample preparation and analysis in a serial process sequence, in particular in the field of liquid chromatography and especially in the area of coupled LC-MS analysis, i. by further coupling with a mass spectrometer.
  • a mixer in the form of a mixer having a shaking head, with at least one receptacle for a sample container, wherein the shaker connected to the motor axis of a stepper motor and the at least one sample container is arranged eccentrically to the motor axis.
  • the movement of the shaking head causing the mixing in the at least one sample container corresponds to a segment of an imaginary shaking circuit, the radius of this shaking circuit being defined by the distance between the motor axis and the axis of the at least one sample container.
  • these steps may include precipitation, liquid-liquid extraction, solid-phase extraction, filtration, derivatization and / or thermal incubation, and in particular small sample quantities for the samples can be mixed with the mixer according to the invention and serially, ie one by one, fed directly to the sample container for further work-up and then to the actual analysis.
  • the mixing-causing movement is effected by a change in the direction of rotation of the motor and the frequency of the direction of rotation of the motor and the amplitude are variably adjustable.
  • these two parameters, frequency and amplitude can be set as desired and also changed during a shaking cycle. In this way, standing waves in the liquid sample to be shaken can be advantageously avoided.
  • the latter interacts with a robot which has at least one arm for loading and unloading the receptacle for the at least one sample container of the mixer.
  • This robot is part of a robot technology and accordingly has an interface to a control unit so that it is controlled and regulated fully automatically.
  • the invention also relates to a method for mixing a sample, with a mixer as already described above, in which a sample container containing the sample is positioned in a receptacle of a shaking head provided for this purpose and the sample by means of motor force by a reciprocating motion is mixed on a segment of a Schicktelnikes whose radius results from the distance between the motor axis and the axis of the at least one sample container.
  • the sample contained in the at least one sample container is not mixed with a circular or circular movement, as is known from the prior art, but by a reciprocating movement.
  • the shaking movement effecting the mixing takes place by a rotation direction change of the motor, and the frequency of the direction of rotation of the motor and the amplitude are changed within a mixing operation in order to avoid standing waves within the sample to be mixed.
  • Particularly effective standing waves in the sample to be mixed can be avoided by the mixing causing shaking movement is made by a change of direction of rotation of the motor, and the frequency of the direction of rotation of the motor and the amplitude can be changed independently within a mixing process.
  • the sample container containing the sample is used from above in approximately perpendicular to the receptacle of the shaking head.
  • the invention further relates to a use of the mixer, as has already been explained above, and the method, as already set forth in its possible steps, for the mixing of small amounts of samples from the biological-medical field.
  • the invention relates to use for automatic sample preparation and analysis in a serial analysis procedure.
  • the present invention is directed to providing a mixer suitable for on-line sample preparation in the sense of serial operation of a fully automated analytical system. This also includes the task of constructing the mixer in such a way that it can be used in the field of on-line sample preparation for the use of a robot technology with the stated aim.
  • a mixer was constructed by reference to Fig. 3 is designated overall by the reference numeral 1.
  • This mixer 1 is called a high energy mixer. It has a shaking head 3, which is firmly connected to the motor shaft 5 of a stepping motor 7.
  • the shaking head 3 four receptacles 9 are provided for sample container 11, which are arranged eccentrically to the axis 5 of the stepping motor 7 according to this embodiment.
  • each positioned in the receptacles 9 sample container 11 are arranged eccentrically to the axis 5 of the stepping motor.
  • Each sample container 11 is inserted from above into the respectively provided receptacle 9 of the shaking head 3.
  • the shaking head 3 is basically on the respective Sample container 11 is tuned and can, if necessary, depending on the type of or the sample container (s) 11, be replaced.
  • the required parameters for the stepping motor 7 are to be determined, which are just as carefully matched to the respective sample container 11, the sample space 13 and the sample itself.
  • the parameters mentioned include the frequency and amplitude for the direction of rotation change of the stepping motor 7, connected to the choice of the angular steps of the stepping motor 7, since the mixing or shaking is done by a change of direction of the stepping motor 7.
  • Direction of rotation switching frequency and amplitude can be set arbitrarily. In principle, this also applies to the number of angular steps and the diameter of the shaking circuit 15.
  • the rotational direction switching frequency and amplitude are also changed during one and the same shaking cycle, thereby avoiding standing waves in the sample to be mixed or shaken.
  • the stepping motor 7 changes its direction of rotation several times per second.
  • the radius of the shaking circuit 15 is basically the distance between the motor shaft 5 and the sample container axis 17.
  • the sample to be mixed is not mixed by means of a circular motion or a circular motion, but by the reciprocating on a segment of the shaker 15th By rapidly changing the direction of rotation and by choosing the angular steps of the stepping motor 7, the desired shaking on the corresponding segment of the shaker 15 results.
  • the illustrated mixer 1 is shown as it is embedded within a complete analysis system for the analysis of samples 19 from the biological-medical field, which are to be examined as part of a screening with integrated fully automatic sample preparation by LC-MS.
  • the samples 19 must first be processed method specific, in order to then be able to perform the actual meter.
  • blood plasma tests have been performed by LC-MS.
  • the blood plasma must be diluted, added to an internal standard and a precipitant added.
  • Each of the samples 19 thus treated is then shaken first by means of the mixer 1 according to the invention and then centrifuged, so that the supernatant can be supplied to the LC-MS as a measurable sample solution.
  • FIG. 1 Such a sample preparation for a liquid-liquid extraction is shown schematically, relates to the representation in Fig. 2 a sample preparation in which a liquid-liquid extraction and a solid phase extraction (SPE) are possible.
  • the blender 1 according to the invention can be used equally in both systems. Accordingly, he is in Fig. 1 with the reference numeral 1 and in Fig. 2 denoted by the reference numeral 1 '. In this case, the mixer 1 is used, as has already been described in detail above, without between the representations of Fig. 1 and 2 to distinguish or to differentiate.
  • the sample to be mixed 19 is positioned in each case via a robot arm in the mixer and then transferred together with the sample container 11 in a downstream centrifuge, which also has a computer-implemented control.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP15000816.7A 2014-03-20 2015-03-19 Mélange haute énergie pour une préparation automatique d'échantillon dans un déroulement d'opérations en série Withdrawn EP2921221A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH00442/14A CH709399A1 (de) 2014-03-20 2014-03-20 Hochenergie-Mixer für eine automatische Probenvorbereitung in einem seriellen Verfahrensablauf.

Publications (1)

Publication Number Publication Date
EP2921221A1 true EP2921221A1 (fr) 2015-09-23

Family

ID=53191438

Family Applications (1)

Application Number Title Priority Date Filing Date
EP15000816.7A Withdrawn EP2921221A1 (fr) 2014-03-20 2015-03-19 Mélange haute énergie pour une préparation automatique d'échantillon dans un déroulement d'opérations en série

Country Status (2)

Country Link
EP (1) EP2921221A1 (fr)
CH (1) CH709399A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2081118A (en) * 1980-08-04 1982-02-17 Technicon Instr Non-invasive mixing
DE3838361A1 (de) * 1987-11-12 1989-05-24 Hitachi Ltd Analysenvorrichtung zum ruehren einer reaktionsloesung und reaktionsbehaelter zur verwendung in der vorrichtung
WO2008038311A1 (fr) * 2006-09-27 2008-04-03 Health Robotics S.R.L. Agitateur mécanique

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009048918A1 (de) * 2009-10-10 2011-04-14 Siemens Healthcare Diagnostics Products Gmbh Vorrichtung zum Mischen einer Flüssigkeitsprobe

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2081118A (en) * 1980-08-04 1982-02-17 Technicon Instr Non-invasive mixing
DE3838361A1 (de) * 1987-11-12 1989-05-24 Hitachi Ltd Analysenvorrichtung zum ruehren einer reaktionsloesung und reaktionsbehaelter zur verwendung in der vorrichtung
WO2008038311A1 (fr) * 2006-09-27 2008-04-03 Health Robotics S.R.L. Agitateur mécanique

Also Published As

Publication number Publication date
CH709399A1 (de) 2015-09-30

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