EP2670530B1 - Contenant de filtration - Google Patents

Contenant de filtration Download PDF

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Publication number
EP2670530B1
EP2670530B1 EP11805425.3A EP11805425A EP2670530B1 EP 2670530 B1 EP2670530 B1 EP 2670530B1 EP 11805425 A EP11805425 A EP 11805425A EP 2670530 B1 EP2670530 B1 EP 2670530B1
Authority
EP
European Patent Office
Prior art keywords
walls
filtration
section
chamber
filter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP11805425.3A
Other languages
German (de)
English (en)
Other versions
EP2670530A1 (fr
Inventor
Christopher Biddell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sartorius Stedim Biotech GmbH
Original Assignee
Sartorius Stedim Biotech GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Stedim Biotech GmbH filed Critical Sartorius Stedim Biotech GmbH
Publication of EP2670530A1 publication Critical patent/EP2670530A1/fr
Application granted granted Critical
Publication of EP2670530B1 publication Critical patent/EP2670530B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
    • B01D35/30—Filter housing constructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

Definitions

  • a concentration of macromolecules, in particular of protein samples, is carried out in a volume range of 500 ⁇ l to 20 ml in filtration containers, which are placed in a filtrate recovery tube and concentrated in centrifugal units by the ultrafiltration method.
  • ultrafiltration it is common practice to provide a retentate chamber as a so-called dead-stop pocket in the filtration containers to prevent dry running of the sample. In this case, the concentrated sample is collected in the dead volume formed by the retentate chamber and recovered by pipetting.
  • a filtration container for the concentration of macromolecules that collects the concentrated macromolecules in conjunction with a filtrate recovery tube and a microconcentrator for concentrating macromolecules from a solution by rotating the microconcentrator in a centrifuge in a retentate container.
  • the known filtration tank consists of an upper portion, an axially adjoining the upper portion central portion and an axially adjacent lower portion.
  • the upper section forms
  • a sample container which is formed as a hollow cylinder and the downwardly tapered central portion forms a filtration chamber having two opposing downwardly converging main walls and two connecting them at their lateral edges connecting walls.
  • One of the main walls forms a filter window for receiving a laminar membrane filter.
  • the adjoining lower section forms a retentate chamber adjoining the lower edge of the filter window, the limiting main and connecting walls of which are planar extensions of the main and connecting walls of the middle section.
  • the US-A-5,310,527 relates to a centrifuge tube for pelletizing solids from suspensions, wherein the solids are to be deposited in the form of a pellet on the wall of the centrifuge tube and separated from the supernatant.
  • the inner volume of the centrifuge tube is divided into upper and lower portions, the lower pelletizing portion being formed by two surfaces including first and second corners.
  • the first corner is acute-angled and the opposite second corner is obtuse-angled.
  • the centrifuge tube should be installed in the centrifuge in order to achieve the separation effect so that the obtuse corner has the greatest distance to the axis of rotation. In this corner, the solids are to be precipitated as pellets.
  • the inner volume of the centrifuge tube is tapered and / or keyhole-shaped.
  • the rejuvenation should serve the adaptation to small-volume suspensions.
  • the disadvantage is that this device does not affect a filtration device and with it no concentration of macromolecules is possible.
  • a substantially hollow cylindrical container has a cylindrical insert which divides the container in the longitudinal direction and has a sealing collar.
  • an obliquely cut approach is arranged, whose inlet opening is covered by a filter membrane, which thus rises by an angle between 25 and 45 °.
  • an annular retentate chamber is formed between the attachment and the surrounding container wall, in which the retentate is collected.
  • the obliquely sloping bottom of the retentate chamber ensures that when the retentate is pipetted out of the retentate chamber, virtually complete sample recovery of the sample or retentate is made possible.
  • the pipette only has to be attached with its tip adjacent to the second connecting wall at the bottom of the retentate chamber. Due to the rounded transition, the flow of the retentate to the lowest point of the retentate container is favored and the complete suction is improved with a pipette.
  • the converging main walls are planar, wherein the connecting walls are extensions of the hollow cylindrical conversion of the upper section. Due to the converging main walls, the emergence or the formation of a small volume retentate chamber is favored.
  • the flat membrane filter on its side facing away from the filtration chamber on a filter cover, which has the lower edge of the filter window adjacent a plurality of juxtaposed above filtrate channels.
  • the filtrate channels are designed to be conically widened to their outer side facing away from the membrane filter.
  • the filtrate channels have, relative to the membrane filter, relatively small openings which form a defined borderline to the filtrate chamber, the enlarged outlet openings of which favor the filtrate flow into the filtrate recovery tube.
  • At least the retentate chamber of the polymer is polystyrene.
  • Polystyrene has the advantage that it is chemically inert and has no protein binding properties.
  • the collection of the concentrated sample or of the retentate is made possible in a very small volume space, which is also chemically inert.
  • the bottom of the retentate chamber is inclined relative to the horizontal lower edge of the filter window by an angle of inclination between greater than 5 ° and 30 °, preferably by approximately 15 °.
  • a filtration tank 1 essentially consists of an upper section 2, a middle section 3 and a lower section 4.
  • the filtration tank 1 can be inserted into a filtrate recovery tube 5, which can be closed with a closure cap 6.
  • the upper portion 2 forms a sample container 7, which is formed as a hollow cylinder and has an inlet opening 9 at its upper end face 8.
  • the upper portion 2 has a circumferential bead 10 which serves as a stop against the filtrate recovery tube 5.
  • the middle section 3 tapers downwards in the vertical direction and forms a filtration chamber 11, which has two main walls 12, 13 facing each other downwards towards each other and two gusset-like connecting walls 14, 15 connecting them at their lateral edges.
  • a filter window 16 for receiving a flat membrane filter 17 is arranged in each of the connecting walls 14, 15.
  • the filter windows 16 each have a transverse to the longitudinal axis 19 of the filtration tank 1 lower edge 18, to which the lower portion 4 connects in the vertical direction downwards.
  • the lower portion 4 forms a retentate chamber 21, the limiting main walls 22, 23 with connecting walls 24, 25 extensions of the main walls 12, 13 and the connecting walls 14, 15 of the filtration chamber 11 are.
  • the retentate chamber 21 is bounded in the vertical direction downwards by a bottom 26.
  • the bottom 26 of the retentate 21 is laterally unilaterally sloping, so that the first connecting wall 24 of the retentate 21 is shorter than its opposite second connecting wall 25.
  • the newly formed bottom 26 is compared to the horizontal lower edge 18 of the filter window 16 by an inclination angle 27 of about 15 ° inclined.
  • the converging main walls 12, 13 each form an angle of inclination 28 of approximately 4 ° with respect to the longitudinal axis 19.
  • the retentate chamber below the lower edge 18 forms a retentate volume 20 (dead space).
  • each the filter window sealingly closing membrane filter 17, which are formed areally arranged.
  • the membrane filters 17 are each covered on their side facing away from the filtration chamber 11 outside of a filter cover 29 connected to the main walls 12, 13.
  • the filter cover 29 engages with an inner shoulder 30 in a circumferential recess 31 of the filter window 16 a.
  • the filter cover 29 has the lower edge 18 of the filter window 16 adjacent to a plurality of juxtaposed filtrate channels 32 above.
  • the filtrate channels 32 are designed to be conically widened to their outer side facing away from the membrane filter 17.
  • the filtration tank 1 is formed of the polymer polystyrene.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (6)

  1. Contenant de filtration (1), en particulier pour la concentration de macromolécules, qui peut être inséré dans un tube d'extraction de filtrat (5),
    comprenant une section supérieure (2), une section centrale (3) dans le prolongement axial de la section supérieure (2) et une section inférieure (4) dans le prolongement axial de la section centrale (3),
    parmi lesquelles
    - la section supérieure (3) forme un contenant à échantillons (7), qui est de forme cylindrique creuse et qui présente sur sa face frontale supérieure (8) un orifice d'entrée (9),
    - la section centrale (3) effilée vers le bas forme une chambre de filtration (11), qui comprend deux parois principales (12, 13) opposées se rejoignant vers le bas ainsi que deux parois de liaison (14, 15) du type gousset reliant lesdites parois principales au niveau des bords latéraux de celles-ci, dans lequel au moins l'une des parois principales (12, 13) comprend une fenêtre de filtre (16) destinée à loger une membrane filtrante plate (17) et
    - la section inférieure (4) forme une chambre de rétentat (21) dans le prolongement du bord inférieur (18) de la fenêtre de filtrat (16), chambre dont les parois principales (22, 23) de délimitation et les parois de liaison (24, 25) sont des prolongements plats des parois principales (12, 13) et des parois de liaison (14, 15) de la section centrale (3), caractérisé
    en ce que le fond (26) de la chambre de rétentat (21) s'incline latéralement d'un côté, de sorte qu'une première paroi de liaison (24) de la chambre de rétentat (21) est plus courte que la deuxième paroi de liaison (25) qui lui est opposée, dans lequel le fond (26) de la chambre de rétentat (21) est incliné d'un angle d'inclinaison (27) compris entre >5° et 30° par rapport au bord inférieur horizontal (18) de la fenêtre de filtre (16) et dans lequel le fond (26) est plat et présente une transition arrondie en direction des parois de liaison (24, 25).
  2. Contenant de filtration selon la revendication 1,
    caractérisé
    en ce que les parois principales (12, 13) se rejoignant sont plates et en ce que les parois de liaison (14, 15) sont des prolongements de la paroi de forme cylindrique creuse de la section supérieure (2).
  3. Contenant de filtration selon l'une quelconque des revendications 1 et 2,
    caractérisé
    en ce que la membrane filtrante plate (17) présente sur sa face extérieure opposée à la chambre de filtration (11) un couvercle de filtre (29) qui présente dans la partie supérieure au voisinage du bord inférieur (18) de la fenêtre de filtre (16) une pluralité de canaux de filtrat (32) juxtaposés.
  4. Contenant de filtration selon la revendication 3,
    caractérisé
    en ce que les canaux de filtrat (32) sont élargis de manière conique en direction de leur face extérieure opposée à la membrane filtrante (17).
  5. Contenant de filtration selon la revendication 1,
    caractérisé
    en ce que la chambre de rétentat (21) est formée du polymère polystyrène.
  6. Contenant de filtration selon la revendication 1,
    caractérisé
    en ce que l'angle d'inclinaison (27) atteint environ 15°.
EP11805425.3A 2011-01-31 2011-12-27 Contenant de filtration Active EP2670530B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE202011002149U DE202011002149U1 (de) 2011-01-31 2011-01-31 Filtrationsbehälter
PCT/EP2011/006576 WO2012103914A1 (fr) 2011-01-31 2011-12-27 Contenant de filtration

Publications (2)

Publication Number Publication Date
EP2670530A1 EP2670530A1 (fr) 2013-12-11
EP2670530B1 true EP2670530B1 (fr) 2018-02-21

Family

ID=45020355

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11805425.3A Active EP2670530B1 (fr) 2011-01-31 2011-12-27 Contenant de filtration

Country Status (5)

Country Link
US (1) US9409109B2 (fr)
EP (1) EP2670530B1 (fr)
JP (1) JP5960168B2 (fr)
DE (1) DE202011002149U1 (fr)
WO (1) WO2012103914A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102013216891A1 (de) * 2013-08-26 2015-02-26 Robert Bosch Gmbh Dreidimensionale, mikrofluidische, durch Zentrifugalkraft antreibbare Vorrichtung, Zentrifuge mit derartiger Vorrichtung und Verwendungen derselben
JP6413972B2 (ja) * 2015-07-31 2018-10-31 株式会社安川電機 検体処理方法及び検体処理システム
US20190001323A1 (en) * 2017-06-30 2019-01-03 Boston Scientific Scimed, Inc. Filtration device with multiple post-filtration orientations
CN107684774B (zh) * 2017-09-29 2023-09-22 广州市雷德生物科技有限公司 一种过滤装置
EP4032603A1 (fr) * 2021-01-22 2022-07-27 Paul Charles Reardon Filtre
WO2022157366A1 (fr) * 2021-01-22 2022-07-28 Paul Charles Reardon Filtre

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60196667A (ja) * 1984-03-21 1985-10-05 Kikkoman Corp 分離用試験器
US4722792A (en) * 1985-02-09 1988-02-02 Kurashiki Boseki Kabushiki Kaisha Filter for centrifugal separator
JPS62176560A (ja) * 1986-01-29 1987-08-03 Kurabo Ind Ltd 遠心分離機用フイルタ−濾過器
JPH0638932B2 (ja) 1985-07-09 1994-05-25 東ソー株式会社 遠心型口過用容器
US5310527A (en) * 1992-12-14 1994-05-10 E. I. Du Pont De Nemours And Company Tube for use in a pelleting centrifuge rotor
SE9301759D0 (sv) 1993-05-21 1993-05-21 Vincenzo Vassarotti Centrifugal method for concentrating macromolecules from a solution and device for carrying out said method
US6156199A (en) * 1997-08-11 2000-12-05 Zuk, Jr.; Peter Centrifugal filtration apparatus
DE69931837T2 (de) 1998-03-20 2006-11-09 China Petrochemical Corp. Hydroentschwefelung von Kohlenwasserstoffölen
SE9900530D0 (sv) 1999-02-15 1999-02-15 Vincenzo Vassarotti A device for concentrating and/or purifying macromolecules in a solution and a method for manufacturing such a device
US6719896B1 (en) * 2002-01-23 2004-04-13 Millipore Corporation Fluid-filtration receptacle with user-variable semi-permeable drain assembly
US8357296B2 (en) * 2007-09-24 2013-01-22 Emd Millipore Corporation Centrifugal filter

Also Published As

Publication number Publication date
WO2012103914A1 (fr) 2012-08-09
DE202011002149U1 (de) 2011-10-26
EP2670530A1 (fr) 2013-12-11
US9409109B2 (en) 2016-08-09
JP5960168B2 (ja) 2016-08-02
JP2014504954A (ja) 2014-02-27
US20130313184A1 (en) 2013-11-28

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