EP2598242B1 - Dispositif de séparation d'éléments constitutifs d'un échantillon liquide - Google Patents

Dispositif de séparation d'éléments constitutifs d'un échantillon liquide Download PDF

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Publication number
EP2598242B1
EP2598242B1 EP11730285.1A EP11730285A EP2598242B1 EP 2598242 B1 EP2598242 B1 EP 2598242B1 EP 11730285 A EP11730285 A EP 11730285A EP 2598242 B1 EP2598242 B1 EP 2598242B1
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EP
European Patent Office
Prior art keywords
chamber
channels
channel
area
separating device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
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EP11730285.1A
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German (de)
English (en)
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EP2598242A1 (fr
Inventor
Tobias Rodenfels
Gert Blankenstein
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Boehringer Ingelheim Microparts GmbH
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Boehringer Ingelheim Microparts GmbH
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Priority to EP11730285.1A priority Critical patent/EP2598242B1/fr
Publication of EP2598242A1 publication Critical patent/EP2598242A1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements

Definitions

  • the present invention relates to a device for the treatment and preferably examination of sample liquid, in particular blood, according to the preamble of claim 1 and such a method.
  • the present invention is particularly concerned with microfluidic systems and devices.
  • the following explanations therefore relate in particular to devices in which capillary forces act and, in particular, are decisive for the function.
  • Devices are known in which blood is filtered as sample liquid by means of a flat separating device.
  • the filtrate or the permeate is taken up in a chamber which adjoins the separator on the flat side and is discharged laterally via a connecting channel.
  • the channel has a relation to the chamber substantially smaller cross-section.
  • fluidic structures such as columns or the like, in order to achieve the most uniform distribution and accordingly good discharge or discharge via the channel.
  • fluidic structures are disadvantageous in terms of a much larger dead volume.
  • Similar devices for blood separation are for example from WO 2005/119211 A1 and the WO 2009/106331 A2 known.
  • the flat, thin separating device can be slightly deformed transversely to its surface extension, in particular in the adjoining the separator downstream chamber.
  • the US5135719 A discloses a method of separating plasma and red blood cells wherein a low pressure filter (specifically, a microfiber glass filter) is disposed in a flow path between an inlet and a reaction area. Under the filter, the plasma collects in a collecting area and flows through capillary forces driven solely by capillary forces to the reaction area. At the reaction area a vent is connected.
  • a low pressure filter specifically, a microfiber glass filter
  • the EP 1500937 A1 discloses an instrument for analyzing fluidic samples wherein a separation sheet separates solid components from the fluid and the filtered liquid distributes from an underlying reservoir into radially outgoing channels. Each of these channels has a branch connected to a vent and an expanded reaction area containing a solid, liquid-soluble reagent.
  • the present patent application is based on the object of specifying a device and a method for the treatment and preferably examination of sample liquid, in particular blood, wherein an optimization of the fluidic throughput is made possible in a simple manner and / or while avoiding an excessively increased dead volume.
  • a basic idea of the present invention is that the sample liquid from the downstream of the separator - especially directly and / or flat - subsequent chamber not only one, but at least two channels and accordingly not only one, but at least two lateral Derive drainage areas.
  • a significantly higher throughput or a significantly larger amount be derived on sample liquid.
  • This can be explained in particular by the fact that a better discharge from different areas of the substantially flat or planar chamber and thus also from different areas of the areal separator takes place. Accordingly, the permeability of the sample liquid separator is better utilized over a wider range than is otherwise the case with only a small channel channel.
  • the sample liquid derived from the two channels that is to say the sample liquid filtered by the separating device, such as blood plasma or blood serum, can then be supplied, for example, to a common examination area or alternatively to two separate examination areas.
  • the channels are preferably brought together in a connecting chamber or connecting line, which is particularly preferably vented in order to avoid undesired gas or air influences, in particular in the case of channels filling at different rates.
  • Fig. 1 shows in a schematic section a proposed device 1 for the treatment and preferably examination of sample liquid 2, in particular blood or other human or animal body fluid.
  • the apparatus 1 has a planar separating device 3 for treating the sample liquid 2, in particular for separating off constituents, such as particles or cells, from the sample liquid 2.
  • the separator 3 is used for filtering. It preferably has a filter element, a membrane or the like. on.
  • the separator 3 may be constructed one or more layers. It is preferably flat or flat.
  • the separating device 3 by a membrane, as in the WO 2009/106331 A2 described, formed or provided with it.
  • the device 1 has a chamber 4 for receiving sample liquid 2 that has flowed through the separating device 3.
  • the chamber 4 thus receives sample fluid 2 treated or filtered by the separating device 3, such as blood plasma or blood serum.
  • the chamber 4 preferably connects flat side and directly to the separator 3 downstream.
  • the chamber 4 is arranged below the separating device 3.
  • the separating device 3 is larger in area than the chamber 4 is formed.
  • the separating device 3 protrudes laterally beyond the chamber 4, in the illustrated embodiment particularly preferably on all sides.
  • the sample liquid 2 is guided free of side walls in the chamber 4. This is achieved in the illustrated example, in particular, that adjoins a bottom 5 of the chamber 4 laterally a capillary stop, which is particularly preferably formed by a circumferential or laterally adjoining trench 6.
  • a capillary stop which is particularly preferably formed by a circumferential or laterally adjoining trench 6.
  • the chamber 4 in particular between the bottom 5 of the chamber 4 on the one hand and the opposite region of Separator 3 formed. Due to the small distance between these two opposite surfaces, the sample liquid 2 is preferably kept free of side walls in the chamber 4 by capillary forces.
  • the trench 6 forms by its abrupt cross-sectional enlargement - ie its greater depth relative to the chamber height (distance of the bottom 5 of the separator 3) - the capillary stop.
  • the device 1 preferably has a carrier 7, an associated cover 8 and / or a receiving element 9.
  • the carrier 7 is preferably plate-like and / or rigid.
  • the carrier 7 is preferably made of plastic and / or by injection molding.
  • the carrier 7 preferably has microfluidic structures for the sample liquid 2 and / or deaeration structures or the like. on, which will be discussed later. These structures are preferably - at least partially - covered by the cover 8.
  • the cover 8 is preferably made of plastic and / or designed as a film.
  • the cover 8 is preferably laminated to the carrier 7 or glued or otherwise connected thereto.
  • the cover 8 preferably extends at least substantially over the entire surface or continuously over the carrier 7.
  • the receiving element 9 preferably serves to hold the separating device 3 and / or to receive the sample liquid 2, such as a blood drop, as in FIG Fig. 1 indicated.
  • the receiving element 9 has for this purpose, for example, a receiving area 10, such as an opening, opening or the like., On.
  • the separator 3 may, for example, in an annular shoulder or the like. be held on the receiving element 9.
  • the separating device 3 is connected, for example, by gluing, clamping and / or welding or in any other suitable manner with the receiving element 9 and / or the carrier 7 or the cover 8 or held thereof.
  • the receiving element 9 is arranged on the cover 8 or connected thereto.
  • the receiving element 9 can also be directly connected to the carrier 7 and / or be formed by this.
  • the trench 6 is connected via a vent channel 11 to the environment.
  • the vent channel 11 is formed in the carrier 7.
  • the sample liquid 2 that has passed through the separator 3 is also referred to as the sample liquid 2.
  • Fig. 1 is merely indicated schematically that the sample liquid 2 upstream of the separator 3 may include, for example, schematically indicated larger components that are no longer contained in the sample liquid 2 after flowing through the separator 3 - ie in the chamber 4. It is in particular in the chamber 4 and further downstream then to the treated or filtered sample liquid 2 or its permeate. The further description is to be understood in particular in this sense.
  • Fig. 2 shows a schematic plan view of a preferred fluidic structure for the discharge of the sample liquid 2 from the chamber 4 of the proposed device 1 according to a first embodiment. In particular, the shows Fig. 2 in a schematic plan view of the carrier 7 without cover 8, receiving element 9, separator 3, sample liquid. 2
  • the device 1 has a first channel 12, which laterally adjoins the chamber 4 in a first outflow region 13.
  • the device 1 further has at least one second channel 14, which laterally adjoins the chamber 4 in a second discharge region 15.
  • the device 1 thus has a plurality of channels 12, 14 and discharge regions 13, 15 for the discharge of sample liquid 2 from the chamber 4.
  • the channels 12, 14 have a relation to the chamber 4 a substantially smaller cross-section.
  • the channels 12, 14 preferably each have a minimum or average cross section or a cross section in the region of the respective outflow region 13/15, which is less than 20%, preferably less than 15%, in particular less than 10%, particularly preferably less than 5 % of the maximum cross section of the chamber 4 is.
  • Maximum cross-section of the chamber 4 is to be understood here in particular as a product of chamber height with the average or maximum diameter of the bottom 5.
  • the channels 12, 14 and drainage areas 13, 15 are preferably offset at the edge of the chamber 4 or arranged or connected on opposite sides. Accordingly, the discharge of sample liquid 2 from the chamber 4 from different areas or at different locations, whereby the throughput of the device 1 and the separation or filter performance of the device 1 and the separator 3 can be significantly increased in a surprisingly simple manner, as already explained at the beginning.
  • more than two channels and drainage areas for the discharge of sample liquid 2 from the chamber 4 to the chamber 4 may be laterally connected.
  • the throughput or the discharge of sample liquid 2 can be further increased.
  • a cross-type arrangement of four channels and outflow areas on the chamber 4 is possible.
  • Fig. 2 It can be seen that the lateral trench 6 is divided by the drainage regions 13, 15 into two trench sections, which surround the chamber 4 or its bottom 5, in particular like an annular segment.
  • the outflow regions 13, 15 form web-like or bridge-like connections via the trench 6 to the chamber 4 or its bottom 5.
  • the channels 12, 14 are preferably formed by groove-like or groove-like depressions, in particular in the carrier 7.
  • the channels 12, 14 extend over the drainage regions 13, 15, particularly preferably into the chamber 4 or the bottom 5 and / or under the separating device 3.
  • the channels 12, 14 end open towards the separating device 3 in their respective end region .
  • the cover 8, which otherwise covers the channels 12, 14, may also, as required, extend into or over the drainage regions 13, 15 and / or into the chamber 4 or over the bottom 5, in particular to form tongue-like protuberances 16, as in FIG Fig. 2 indicated by dash-dotted lines.
  • the channels 12, 14 extend at least substantially to one another in the middle of the chamber 4 or in the bottom 5, as in FIG Fig. 2 indicated by dashed lines.
  • the device 1 has a preferably chamber-like examination area 17 for the examination of sample liquid 2 derived from the chamber 4.
  • both the first channel 12 and the second channel 14 are fluidly connected to the examination area 17 in the illustrated example. This can optionally be done directly or optionally via a connecting line 18 and / or a connecting chamber 19, as in Fig. 2 indicated.
  • the connection line 18 and the connecting chamber 19 are thus in particular optional and can also be omitted if necessary.
  • the two channels 12 and 14 initially combine and the sample liquid 2 derived from the chamber 4 is then forwarded to the examination region 17 via the common connection line 18.
  • the optional connecting chamber 19 is preferably formed. As I said, but this is only optional and can be omitted if necessary.
  • a vent for the examination area 17 and / or for the connecting line 18 and / or connecting chamber 19 is provided.
  • the venting of connecting line 18 and connecting chamber 19 is preferably carried out in the illustrated embodiment by a vent channel 20, for example, directly or indirectly connected to the environment and / or may be in gas exchange and / or with the examination area 17 and / or trench 6 for venting or can be connected.
  • the venting provided is preferably designed such that, even with differently fast filling of the channels 12, 14 with sample liquid 2 derived from the chamber 4, no unwanted gas or air inclusion occurs in the examination area 17 or in the sample area 2 supplied to the examination area 17.
  • the vent is preferably also by groove-like or groove-like depressions and / or openings or the like. formed in the carrier 7 and / or, if necessary, in the cover 8.
  • the connecting chamber 19 preferably serves primarily only for venting and therefore has a preferably only minimal volume.
  • Fig. 3 make one Fig. 2 Some aspects, such as the optional venting and the possible protuberances 16 of the cover 8 in the discharge areas 13, 15 and chamber 4, have been omitted, but can be realized accordingly.
  • the two channels 12, 14 are connected to separate examination areas 17 and 21.
  • the device 1 thus has, in addition to the (first) examination area 17, a further or additional examination area 21, which is supplied with sample fluid 2 by the second passage 14.
  • connection line 18 or connection chamber 19 may also be connected to a further separate examination area 21 in addition to the examination area 17 in order to separate or divide the sample liquid 2 removed from the chamber 4 again after the channels 12, 14 have been combined.
  • the device 1 is in particular a microfluidic device.
  • the volumes of the device 1 or individual or all microfluidic structures, such as the chamber 4, the channels 12, 14, the connecting line 18, the connecting chamber 19 and / or the examination areas 17, 21 are preferably less than 1 ml, in particular less than 500 .mu.m, more preferably substantially 100 .mu.l or less.
  • the volume of the channels 12, 14 is in each case preferably less than 20%, in particular less than 10%, of the volume of the chamber 4.
  • the preferred derivation of the sample liquid 2 from the chamber 4 on the other hand in cross section small, but several channels 12, 14 has the advantage that even with minimal volume of the chamber 4, in particular minimum chamber height or possibly little or no microstructuring of the chamber 4 or of the chamber bottom 5 for minimizing the dead volume, a good throughput or a high separation efficiency can be achieved.
  • a treatment and in particular examination of the sample liquid 2 can take place even in the event of failure or obstruction of a channel 12 or 14, when the two channels 12, 14 are fluidically connected to a common examination area.
  • the use of a plurality of channels 12, 14 can also achieve a faster filling of the examination areas 17 and 21 compared to the prior art.
  • the channels 12 and 14 preferably extend at least substantially in the main extension plane of the chamber 4 or the carrier 7 and / or in a plane parallel thereto.
  • the device 1 can in particular for examining the sample liquid 2 or for determining an analyte in the sample liquid 2 or the like. be used.
  • the device 1 for realizing a Imuno assay reaction or the like serve.
  • the device 1 can serve for the determination or analysis of specific analytes or other values of the sample liquid 2.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (13)

  1. Dispositif (1) de traitement et de préférence d'analyse d'échantillon liquide (2), en particulier de sang, avec un support (7),
    avec un dispositif de séparation plat (3),
    avec une chambre (4) en aval, se raccordant côté plat au dispositif de séparation (3), pour la réception d'un échantillon liquide (2) qui a traversé le dispositif de séparation (3), dans lequel la chambre (4) est agencée sous le dispositif de séparation (3) et le dispositif de séparation (3) est réalisé plat plus grand que la chambre (4),
    avec un premier canal (12) et un deuxième canal (14), qui se raccordent dans une première zone d'évacuation (13) et dans une deuxième zone d'évacuation (15) latéralement à la chambre (4) et présentent une section transversale plus petite que la chambre (4), et
    avec une zone d'analyse (17), à laquelle le premier canal (12) et/ou le deuxième canal (14) est raccordé fluidiquement pour conduire un échantillon liquide (2) de la chambre (4) à la zone d'analyse (17),
    caractérisé en ce
    qu'un arrêt capillaire sous la forme d'un fossé (6) périphérique de type segment se raccorde latéralement à un fond (5) de la chambre (4), dans lequel le fossé est raccordé à l'environnement par le biais d'un canal de purge (11) dans le support (7), et
    les zones d'évacuation (13, 15) forment des liaisons de type passerelle ou de type pont au-dessus du fossé (6) vers la chambre (4) et les canaux (12, 14) s'étendent au-dessus des zones d'évacuation (13, 15) jusqu'au milieu de la chambre (4).
  2. Dispositif selon la revendication 1, caractérisé en ce que le deuxième canal (14) est raccordé fluidiquement à la zone d'analyse (17) conjointement avec le canal (12) pour conduire un échantillon liquide (2) de la chambre (4) à la zone d'analyse (17).
  3. Dispositif selon la revendication 1 ou 2, caractérisé en ce que le dispositif présente une conduite de raccordement (18) et/ou une chambre de raccordement (19), à laquelle les deux canaux (12, 14) sont raccordés fluidiquement.
  4. Dispositif selon la revendication 3, caractérisé en ce que le dispositif présente une purge pour la conduite de raccordement (18) ou chambre de raccordement (19).
  5. Dispositif selon la revendication 3 ou 4, caractérisé en ce que la conduite de raccordement (18) ou chambre de raccordement (19) est raccordée à la zone d'analyse (17) pour l'amenée d'échantillon liquide (2).
  6. Dispositif selon la revendication 1, caractérisé en ce que le deuxième canal (12) est raccordé fluidiquement à une zone d'analyse séparée supplémentaire (21) pour conduire un échantillon liquide (2) de la chambre (4) à la zone d'analyse supplémentaire (21).
  7. Dispositif selon l'une quelconque des revendications précédentes, caractérisé en ce que les deux zones d'évacuation (13, 15) sont agencées ou raccordées de manière décalée côté bord au niveau de la chambre (4).
  8. Dispositif selon l'une quelconque des revendications précédentes, caractérisé en ce que les deux zones d'évacuation (13, 15) sont agencées ou raccordées sur des côtés opposés au niveau de la chambre (4).
  9. Dispositif selon l'une quelconque des revendications précédentes, caractérisé en ce que les deux canaux (12, 14) s'étendent jusque sous le dispositif de séparation (3).
  10. Dispositif selon l'une quelconque des revendications précédentes, caractérisé en ce que les deux canaux (12, 14) sont formés par des évidements en particulier en forme de rainure ou de cannelure dans un fond (5) de la chambre (4) et/ou dans les zones d'évacuation (13, 15).
  11. Dispositif selon la revendication 10, caractérisé en ce qu'un recouvrement (8) s'étend, en particulier à la manière d'une languette, au-dessus des zones d'évacuation (13, 15) et/ou sous le dispositif de séparation (3) et y recouvre les canaux (12, 14).
  12. Dispositif selon l'une quelconque des revendications précédentes, caractérisé en ce que les deux canaux (12, 14) sont formés au moins en partie par des évidements en particulier de type rainure ou cannelure dans un support (7) du dispositif (1), recouvert par un recouvrement (8).
  13. Dispositif selon l'une quelconque des revendications précédentes, caractérisé en ce que les deux canaux (12, 14) sont reliés l'un à l'autre sous le dispositif de séparation (3).
EP11730285.1A 2010-07-27 2011-07-05 Dispositif de séparation d'éléments constitutifs d'un échantillon liquide Active EP2598242B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP11730285.1A EP2598242B1 (fr) 2010-07-27 2011-07-05 Dispositif de séparation d'éléments constitutifs d'un échantillon liquide

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10007768 2010-07-27
PCT/EP2011/061307 WO2012019829A1 (fr) 2010-07-27 2011-07-05 Procédé et un dispositif de séparation d'éléments constitutifs d'un échantillon liquide
EP11730285.1A EP2598242B1 (fr) 2010-07-27 2011-07-05 Dispositif de séparation d'éléments constitutifs d'un échantillon liquide

Publications (2)

Publication Number Publication Date
EP2598242A1 EP2598242A1 (fr) 2013-06-05
EP2598242B1 true EP2598242B1 (fr) 2018-11-14

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EP11730285.1A Active EP2598242B1 (fr) 2010-07-27 2011-07-05 Dispositif de séparation d'éléments constitutifs d'un échantillon liquide

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US (1) US8974751B2 (fr)
EP (1) EP2598242B1 (fr)
JP (1) JP5850373B2 (fr)
WO (1) WO2012019829A1 (fr)

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Publication number Priority date Publication date Assignee Title
JP1530328S (fr) * 2015-02-17 2015-08-03

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4753776A (en) * 1986-10-29 1988-06-28 Biotrack, Inc. Blood separation device comprising a filter and a capillary flow pathway exiting the filter
US5135719A (en) 1986-10-29 1992-08-04 Biotrack, Inc. Blood separation device comprising a filter and a capillary flow pathway exiting the filter
CN1650173B (zh) * 2002-04-30 2012-06-06 爱科来株式会社 分析用具、使用分析用具的试样分析方法及分析装置、和分析用具的开口形成方法
DE102004027422A1 (de) 2004-06-04 2005-12-29 Boehringer Ingelheim Microparts Gmbh Vorrichtung zur Aufnahme von Blut und Abtrennung von Blutbestandteilen
US7682817B2 (en) * 2004-12-23 2010-03-23 Kimberly-Clark Worldwide, Inc. Microfluidic assay devices
WO2007090620A2 (fr) 2006-02-10 2007-08-16 Boehringer Ingelheim Microparts Gmbh Dispositif et procédé de traitement ou d'épuration d'échantillons, notamment d'acides nucléiques
US7872735B2 (en) 2007-07-06 2011-01-18 Jds Uniphase Corporation Method and apparatus for referencing a MEMS device
BRPI0907148B1 (pt) 2008-02-27 2021-01-12 Boehringer Ingelheim Microparts Gmbh dispositivo para a separação do plasma
CN101965225B (zh) * 2008-03-11 2014-04-30 皇家飞利浦电子股份有限公司 用于过滤流体的过滤装置

Non-Patent Citations (1)

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Title
None *

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Publication number Publication date
WO2012019829A1 (fr) 2012-02-16
US20130202500A1 (en) 2013-08-08
EP2598242A1 (fr) 2013-06-05
JP5850373B2 (ja) 2016-02-03
US8974751B2 (en) 2015-03-10
JP2013535673A (ja) 2013-09-12

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