EP2587249B1 - Analysis and reading device and analysis and reading method - Google Patents
Analysis and reading device and analysis and reading method Download PDFInfo
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- EP2587249B1 EP2587249B1 EP11867578.4A EP11867578A EP2587249B1 EP 2587249 B1 EP2587249 B1 EP 2587249B1 EP 11867578 A EP11867578 A EP 11867578A EP 2587249 B1 EP2587249 B1 EP 2587249B1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Description
- The present invention relates to the field of assay detection, in particular to a method and device for analyzing and reading in assay detection.
- There have been many devices for analyzing assay substances in the market, and particularly, there are also some related invention patents. For example,
EP291194 EP653625 EP291194 -
US5837546 discloses an automatic assay device and method. The device provides an additional electrode by a cross-flow carrier, detects if there is fluid on the carrier, generates a signal to turn on the electronic assay device, and displays the testing result. However, in different tests, the liquid flows along the carrier at different speeds, and different types of flow rate characteristics of liquids will result in inaccuracy readings. Due to the inconsistent characteristics of the materials of the wick and the porous, water-permeable film, the best time for reading will be different. -
CN 1573315B discloses a device for reading the analytical results performed by a liquid transporting carrier. This device is directed to solve the problem of how to determine the best reading time, but it cannot solve the problem of different fluids having different flow-rate standards. Meanwhile, for some assay devices, their required detection times are too long, for example, an assay-based, commercial cross-flow detection tool for detecting heart injury takes up to 15 minutes to finish the assay. -
CN 1573316B discloses an optical device for assay reading, which also includes a light source and an optical detector, but the device is somewhat complicated and has many more optical paths, thus it is prone to misfortunes which then affect the results. So, the device does not achieve the purpose of optimization. -
EP 1.484.601 discloses another optical arrangement for an assay reading device. - Therefore, it is necessary to provide a device and method for analysis and reading which have simple detection optical path, less interference, simple structure of detection circuit, and accurate readings.
- An objective of the present invention is to provide an analyzing and reading device with simple structure of detection circuit and accurate readings.
- A further objective of the present invention is to provide an analyzing and reading method which is accurate in reading.
- To achieve the object of the present invention, the following technical solution is provided:
An analyzing and reading device, for reading and analyzing a test strip for assay detection, the test trip having a detection zone and a blank zone, comprises: - a processor; and
- a photoelectric detection circuit, configured to detect signals of the strength of light reflection in the detection zone and the blank zone and feedback detection information to the processor; wherein the photoelectric detection circuit comprises:
- at least two light sources, corresponding to positions of the detection zone and the blank zone of the test strip, and able to emit lights corresponding to the detection zone and blank zone of the test strip; and
- at least one optical detector, configured to receive reflected lights from the detection zone and the blank zone;
- wherein the lights emitted by the at least two light sources irradiate the detection zone and blank zone of the strip and are reflected therefrom, and then received by the optical detector, which in turn feedback the detection information to the processor.
- With the analyzing and reading device, an optimal optical circuit path between the light sources and the optical detector is provided. The optical path is short, the structure of the device is simple, relatively stronger signals can be retained, no light compensation need to be done, and by the set of the processor, the obtained signals of light strength are transformed into discriminant values for determining and comparing.
- In theory, the analyzing and reading device disclosed by the present invention may comprises any number of light sources and any number of optical detectors. For instance, in one embodiment, there may be three light sources, each of them irradiates a corresponding zone of the test strip, three zones may share a common detector, and the detection order is in accordance with the order of light emission of the sources.
- In a preferred embodiment, the analyzing and reading device may include a shared optical detector. According to the same principle, the number of the optical detectors may be increased or decreased based on specific test needs.
- Preferably, the light sources may be light-emitting diodes; the optical detector is a photoelectric sensor.
- The analyzing and reading device adopt two light sources, i.e. a first light source and a second light source, wherein the lights emitted by the two sources irradiate the detection zone and blank zone of the test strip, and then are received by the photoelectric sensor after reflection. Preferably, the first and second light sources may be all light-emitting diodes. The first and second light sources separately irradiate the corresponding detection zone and blank zone respectively, wherein the two light sources emit lights successively, and then the reflected lights are received by the common photoelectric sensor; thus, the structure is simpler, the interference is less, and the detection results are more accurate.
- Preferably, the first and second light sources may be all green light-emission diodes (green LED), and under the condition of the assay reaction, the emitted lights irradiate the colored detection zone and the blank zone respectively, then a relatively larger contrast of reflection effect is obtained, which increases the accuracy of the readings, especially when the detection zone after reaction is red.
- Preferably, the wavelengths of the first and second light sources may be the same.
- Preferably, the at least two light sources may emit lights at different times, while the photoelectric sensor receives reflected lights from different zones also at different times.
- Preferably, when the analyzing and reading device includes a plurality of light sources, these light sources are optimally set, such that each light source only irradiates a specific zone, and there is a diaphragm between the light sources and between the light sources and the optical detector, so as to restrict the influences among the strip zones irradiated by each of the light sources and among the light sources, and eliminate the impact of the detected lights.
- The analyzing and reading device further comprises a T-shaped diaphragm which separates the first, second light sources and the photoelectric sensor from one another, so as to avoid light interferences between the blank zone and the detection zone and between a light-emitting zone and a light-receiving zone.
- The two light sources and the photoelectric sensor locate on one side opposite to the detection zone and blank zone, wherein the two light sources are in the same column, while the photoelectric receiver locates opposite to the two sources, and a T-shaped diaphragm is provided between the sources and the sensor, so as to prevent the interference between lights with same or different wavelengths from different light sources. This arrangement makes that, when in the use, lights from different light sources may successively irradiate the detection zone and the blank zone respectively, and the photoelectric sensor successively receives lights diffusely reflected from different light sources. The photoelectric sensor generates a voltage in a positive linear correlation with the strength of the light irradiated thereon. The voltage is caused by the accumulation of the marker, and at the same time, it is also dependent on the amount of the assay substance contained in the sample; then, after detecting the amount existed in the sample, the processor calculates a discriminant value based on the calculation principle of discriminant value, and compares the value with a threshold. The reflected lights may be measured by the photoelectric sensor, wherein the reflected lights refer to lights entered into the optical detector (photoelectric sensor) after the lights from the light sources being reflected by the test strip.
- The humidity of the test strip will be various with the passage of time. In the present invention, after detecting the strength of light, the discriminant value is calculated by setting a calculation formula, and then compared with changing threshold, so as to achieve an optimal discriminant result.
- In the above arrangement, preferably, the light sources are light-emitting diodes, and preferably the optical detector is a photoelectric sensor; a processor is provided for transforming a light-strength signal into a discriminant value that can be compared.
- Preferably, there is a gap between the T-shaped diaphragm, which locates among the first light source, the second light source and the photoelectric sensor, and the detection zone and blank zone of the test strip. Lights emitted by the first and second light sources irradiate the detection and blank zones of the test strip, and then the reflected lights enter the photoelectric sensor through the gap and are received by the sensor. The photoelectric sensor and the light sources are located on the same side. The reflected lights mainly are those lights diffusely reflected through the gap of the diaphragm among the light sources and the photoelectric receiver.
- Signals accumulated during the detection procedure include the forming or accumulating of materials easily to be detected (e.g. the result of color reaction). Specifically, the assay preferably includes the accumulation of a marker, usually the accumulation of the marker in the detection zone. The marker may be colored particles like enzyme, radioisotope tracer, fluorescein, colloidal gold, color latex, etc.
- Usually, certain assay substance in the sample may cause the accumulation of the signals; however, in some cases, for example, in a competitive reaction, the assay substance will not cause the accumulation of the related signals. The reaction that causes the accumulation of signals may be any suitable reaction, such as conventional chemical reaction between two chemical entities, enzyme-linked reaction, or immunoconjugation reaction. The preferable immunoconjugation reaction will include at least one conjugation of biological molecules.
- The preferable reactions include the conjugation of the marker composite body with the specific reagent of the test strip fixed on the detection zone, and the marker accumulates in the conjugation area.
- Usually, the blank control zone only serves as an area of "background" signal, for example, such signal can be used for calibrating the analyzing and reading device and /or provide a referable background signal.
- Preferably, the test strip comprises a porous, water permeable carrier, which includes a specific conjugation reagent with marker and a specific conjugation reagent without marker.
- The analyzing and reading device detects the accumulation amount of the marker, wherein the detection signal is proportional to the accumulation amount of the marker, and according to the calculation formula of discriminant value, the discriminant value is also proportional to the accumulation amount of the marker. The analyzing and reading device can measure optical properties, such as the amount of reflected lights in the detection zone or the blank zone. The reflected lights mean the lights reflected from the porous, water-permeable carrier or other liquid-delivering means to the photoelectric sensor.
- Preferably, the analyzing and reading device further includes a housing made of opaque (i.e. light-tight) synthetic plastic, which is usually a synthetic plastic material, such as ABS, polystyrene, etc.
- Preferably, the housing of the analyzing and reading device has a pore for receiving at least part of the test strip in the internal of the analyzing and reading device; the positions, shapes and sizes of the pore, the light sources and the optical detector are set so that after the installation of the test strip, lights emitted by the light sources incident to the detection zone and the blank zone, and are reflected therefrom to form the reflected lights, which then incident to the photoelectric sensor and make the sensor generate an assay signal representing the amount of the analyte in the zones. The test strip is fixed in the pore, wherein the test strip comprises a sample-sucking bar, wherein a half of the bar exposes outside of the pore, and another half of the bar is inside the housing of the analyzing and reading device; the outside part of the sample-sucking bar is used for sucking the sample liquid, and the detection zone and blank zone provided on the test strip are inside the housing of the analyzing and reading device, opposite to the light sources and the optical detector of the photoelectric detection circuit. The test strip is fixed inside the analyzing and reading device, so as to avoid inaccuracy caused by the movement of the strip, and the strip need not to be re-positioned.
- The sample-sucking bar of the test strip may be any conventional test sample-sucking bar of cross-flow type, preferably include a porous, water-permeable carrier, which comprises a specific reagent combined with a marker and a specific conjugation reagent not having marker.
- Preferably, the marker may be colored particle.
- Preferably, the light source and the optical detector may be configured as not beyond 0.5 cm2, that complies with the demand of volume miniaturization.
- The present invention also provides an analyzing and reading method which uses the above-mentioned analyzing and reading device, wherein the method comprises the following steps: the processor respectively controls the respective lightening of the at least two light sources according to a timing design; after irradiating the detection zone and the blank zone of the test strip, the lights are reflected and then received by an optical detector; then the optical detector feedbacks the detection information to the processor; and, the processor makes analysis and decision based on the detection information received.
- The timing design of the processor may be implemented by software.
- The analyzing and reading device provides an optimal optical path between the light source and the optical detector, and obtains a signal of light strength; the processor is set so that the signal will be transformed into a discriminant value for determining and comparing. Such optical-path-optimizing device and reading method can be used in other similar spectrometric detection equipment.
- Compared with the prior art, the present invention has the following advantages:
The analyzing and reading device of the present invention is simple, economical and practical; based on the selected material, the overall cost is relatively low; using the device and method for analysis and reading according to the present invention, there is less interference when reading and a high accuracy will be achieved. -
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Fig. 1 is an exploded view for showing the structure of the analyzing and reading device according to an embodiment of the present invention; -
Fig. 2 schematically illustrates the distribution of the light sources and the photoelectric sensor of the analyzing and reading device according to an embodiment of the present invention; -
Fig. 3 schematically illustrates the T-shaped diaphragm of the analyzing and reading device according to an embodiment of the present invention; -
Fig. 4 schematically illustrates the combination of the T-shaped diaphragm with the light sources and the photoelectric sensor of the analyzing and reading device according to an embodiment of the present invention; -
Fig. 5 schematically illustrates the light refection principle in the detection zone and the blank zone of the present invention; -
Fig. 6 schematically illustrates the structure of the photoelectric detection circuit according to an embodiment of the present invention; -
Fig. 7 is a schematic flow diagram of the analyzing and reading method according to an embodiment of the present invention. - Referring to
Fig. 1 , in this embodiment, the analyzing and reading device of the present invention specifically includes a processor 1 (CPU) or microcontroller, atest strip 2, a photoelectric detection circuit (not shown), abase board 3, abracket 4, and a housing. Theprocessor 1 and the photoelectric detection circuit are provided on thebase board 3. Thestrip 2 is provided on one side of theracket 4, while thebase board 3 locates on the other side of theracket 4. Adetection window 41 is provided in theracket 4. A detection zone and a blank zone are provided on thetest strip 2. The photoelectric detection circuit and the detection, blank zones locate respectively on both sides of thedetection window 41 and corresponding to each other. The photoelectric detection circuit comprises two LEDs (served as light sources) and a photoelectric sensor; wherein lights emitted by the two LEDs respectively irradiate the detection zone and the blank zone through thedetection window 41, and after being reflected, the lights are received by the photoelectric sensor; the processor is configured to detect the output of the photoelectric sensor, so as to determine the accumulation amount of the signal, then calculate a discriminant value, and finally compare the discriminant value with a threshold. -
Desiccant 40 is provided on theracket 4 in order to keep the drying in the device, and to guarantee the accuracy of the detection. - In this embodiment, the electric power source used in the analyzing and reading device is a
3V button battery 30. In this embodiment, the housing includes anupper housing 51, alower housing 52, afront cover 53 and a battery cover 54. Theprocessor 1, thetest strip 2, the photoelectric detection circuit, thebase board 3 and theracket 4 are placed in the housing. The battery cover 54 can be separately opened to replace the battery. In a preferred embodiment, the housing is about 15 cm in length, about 2.5 cm in width, about 1.5 cm in height, and made of light-tight material, usually of synthetic plastic material (such as ABS, polystyrene, etc.). - Referring to
Figs. 2-5 , the photoelectric detection circuit comprises twogreen LEDs 61, 62 (respectively served as the first, second light sources) and aphotoelectric sensor 63, wherein the emitted lights irradiate thedetection zone 21 and theblank zone 22 of thetest strip 2, and after being reflected, the lights are received by thephotoelectric sensor 63. The light sources and the photoelectric sensor are configured to not exceed 0.5 cm2, which complies with the demand of volume miniaturization. - The two
LEDs photoelectric sensor 63 are separated to one another by a T-shapeddiaphragm 42 positioned in thedetection window 41, so as to prevent light interference between theblank zone 21 and thedetection zone 22, and between light-emitting zone and light-receiving zone. On one side of thedetection window 41 there are the twoLEDs photoelectric sensor 63, while on the other side there are theblank zone 21 and thedetection zone 22. The two light sources are in a same column, and the photoelectric receiver locates opposite to the two light sources; they are separated by the T-shaped diaphragm, so as to prevent light interference between the lights of the two deferent light sources, wherein the lights have same or different wavelengths. - The head a of the T-shaped
diaphragm 42 is slightly lower than the other part of the diaphragm, so as to form a gap between thediaphragm 42 and thetest strip 2. The twoLEDs detection zone 21 and theblank zone 22. The two light sources successively emit lights which irradiate thedetection zone 21 and theblank zone 22 and are then reflected. The reflected lights are received by the commonphotoelectric sensor 63 through a gap between the T-shapeddiaphragm 42 and thetest strip 2; the photoelectric sensor and the light sources are positioned at the same side. The reflected lights mainly are the lights diffusely reflected via the gap of the diaphragm among the light sources and the photoelectric receiver. Such structure is simpler, and has less interference and more accurate detection result. - A pore (not shown), which can be covered by a
front cover 53, is provided in the front end of the housing of the analyzing and reading device. Thetest strip 2 is fixed in the pore; the test strip comprises a sample-suckingbar 23. A half of the bar is exposed outside the pore and the other half of the bar is positioned inside the housing of the analyzing and reading device. The outside part of the sample-suckingbar 23 is used for sucking sample liquid. Thedetection zone 21 and theblank zone 22 provided on the test strip are positioned inside the housing of the device, opposite to the light sources and the optical detector of the photoelectric detection circuit. Thetest strip 2 is fixed in the analyzing and reading device, particularly fixed on theracket 4, to avoid inaccuracy caused by the movement of the test strip, and the test strip need not to be re-positioned. - The sample-sucking
bar 23 of the test strip may be any conventional cross-flow test sample-sucking bar, preferably comprises a porous, water-permeable carrier, which includes a specific conjugation reagent having marker and a specific conjugation reagent not having marker. The analyzing and reading device detects the accumulation amount of the marker, wherein the detection signal is proportional to the accumulation amount of the marker, and according to the calculation formula of discriminant value, the discriminant value is also proportional to the accumulation amount of the marker. The analyzing and reading device can measure optical properties, such as the amount of the reflected lights in the detection zone or the blank zone. The reflected lights mean the lights reflected from the porous, water-permeable carrier or other liquid-delivering means to the photoelectric sensor. - The marker is colored particles, which may be enzyme, radioisotope tracer, fluorescein, colloidal gold, color latex, etc.
- As a preferred embodiment, a liquid crystal display connected to the processor for outputting the working condition and determination result of the analyzing and reading device is provided on the
upper housing 51 of the device. - Referring both to
Figs. 6 and7 , the twoLEDs - The present invention also provides a analyzing and reading method which uses the above-mentioned analyzing and reading device; Referring to
Fig. 7 , the method comprises the following steps: the processor controls the lighting of at least two light sources respectively according to a timing design; after irradiating the detection zone and the blank zone of the test strip, lights are reflected and then received by an optical detector; the optical detector feedbacks the detection information to the processor; the processor makes analysis and decision based on the detection information received. - The timing design of the processor may be realized by software.
- Usually, certain analyte in the sample may cause the accumulation of the signals; however, in some cases, for example, in a competition reaction, the certain analyte will not cause the accumulation of the related signals. The reaction that causes the accumulation of signals may be any suitable reaction, such as conventional chemical reaction between two chemical entities, enzyme-linked reaction, or immunoconjugation reaction. The preferable immunoconjugation reaction will include at least one conjugation of biological molecules.
- After leaving the factory, the device is in a dormant state in order to reduce energy consumption; only when the sample has been sucked by the test sample-sucking bar, and the flow of the sample liquid in the sample-sucking bar induces the change of the electrical resistance of the circuit, the device will be automatically activated from the "dormant" state to a working state.
- In use, the front end of the sample-sucking bar is directly inserted into the sample; after the addition of the sample, the device will be activated; then, a
left electrode 11 and aright electrode 12 of the photoelectric detection circuit are conductively connected, so as to turn on the device and start a normal measurement; after waiting 8 seconds, the device enters the work mode, this time, the two LEDs successively emit lights, and the reader starts to operate; corresponding voltages are detected by the photoelectric sensor and sent to the processor; the signals detected at this moment are initial values of the detection zone and the blank zone, which are used as reference signals before sample addition. After waiting N seconds, the detection operation is repeated, and the signals detected at this moment are served as result determining signals, which are then combined with corresponding algorithm to achieve detection results; wherein the algorithm can be referred to general operations in the art and is not described here. The light-strength signals are received according to the chronological order of the lightening of the light sources. The initial values are scanned in order to use them as the background ones for calculating discriminant values. - During operation, the sample can also be directly poured on the sample-sucking bar; the pouring should be done carefully to not get the other parts wet; the device is taken out and placed in a flat position with the liquid crystal display facing up when a funnel-shaped symbol displayed on the observing window starts to flash (at the same time, a buzzer starts to ring); or, the sample is collected into a clean, single-use container or clean container, and then the sample-sucking bar of the detection pen is inserted into the sample with at least three-fourths of the bar being immerged below the liquid level of the sample; the detection pen of the device is taken out and placed in a flat position with the liquid crystal display facing up when the funnel-shaped symbol displayed on the observing window starts to flash (at the same time, a buzzer starts to ring). According to a pre-set procedure, the device starts to measure one or more values for conducting the comparing.
- The analyzing and reading device provides an optimal optical path between the light source and the optical detector, and obtains a signal of light strength; the processor is set so that the signal will be transformed into a discriminant value for determining and comparing. Such optical-path-optimizing device and reading method can be used in other similar spectrometric detection equipment.
- The above mentioned relates only to preferred embodiments of the present invention, which do not limit the protection scope of the present invention. All equivalent transformations based on the technical solutions of the present invention belong to the protection scope of the present invention.
Claims (10)
- An analyzing and reading device, for reading and analyzing a test strip (2) for assay detection, the test strip (2) having a detection zone (21) and a blank zone (22), comprising:a processor (1);a photoelectric detection circuit, configured to detect signals of the strength of light reflection in the detection zone (21) and the blank zone (22) and feedback detection information to the processor (1); wherein the photoelectric detection circuit comprises:at least two light sources (61, 62), corresponding to positions of the detection zone (21) and the blank zone (22) of the test strip (2), and able to emit lights corresponding to the detection zone (21) and blank zone (22) of the test strip (2); andat least one optical detector, configured to receive reflected lights from the detection zone (21) and the blank zone (22);wherein the lights emitted by the at least two light sources (61, 62) irradiate the detection zone (21) and blank zone (22) of the strip (2) and are reflected therefrom, and then received by the optical detector, which in turn feedback the detection information to the processor (1);the device further comprising a diaphragm (42) separating the at least two light sources (61, 62) and the at least one optical detector from one another,characterized in that the optical detector is a photoelectric sensor (63),the at least two light sources are comprised of a first light source (61) and a second light source (62), wherein the lights emitted by the two light sources (61, 62) irradiate the detection zone (21) and blank zone (22) of the test strip (2), and then are received by the photoelectric sensor (63) after reflection, and the analyzing and reading device further comprises a T-shaped diaphragm (42) separating the first, second light sources (61, 62) and the photoelectric sensor (63) from one another.
- The analyzing and reading device according to claim 1, wherein both the first and second light sources (61, 62) are light emitting diodes.
- The analyzing and reading device according to claim 2, wherein both the first and second light sources (61, 62) are green light emitting diodes.
- The analyzing and reading device according to claim 3, wherein wavelengths of the first and second light sources (61, 62) are the same.
- The analyzing and reading device according to claim 1, wherein the at least two light sources (61, 62) emit lights at different times.
- The analyzing and reading device according to claim 1, wherein there is a gap between the T-shaped diaphragm (42) among the first light source, the second light source and the photoelectric sensor, and the detection zone (21) and blank zone (22) of the test strip (2); the lights emitted by the first and second light sources (61, 62) irradiate the detection (21) and blank (22) zones of the test strip (2), and then the reflected lights enter the photoelectric sensor (63) through the gap and are received by the sensor.
- The analyzing and reading device according to claim 1, wherein the device further comprises a housing made of light-tight synthetic plastic.
- The analyzing and reading device according to claim 1, wherein the test strip (2) comprises a porous water permeable carrier, which comprises a specific conjugation reagent with marker and a specific conjugation reagent without marker.
- The analyzing and reading device according to claim 8, wherein the marker is colored particles.
- An analyzing and reading method for using the analyzing and reading device according to claim 1, comprising the following steps: controlling by the processor (1) the respective lightening of the at least two light sources (61, 62) according to a timing design to irradiate the detection zone (21) and the blank zone (22) of the test strip (2); receiving by the optical detector lights reflected; then feeding back by the optical detector detection information to the processor (1); and, making analysis and determination by the processor (1) according to the detection information received.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110223580A CN102323215A (en) | 2011-08-05 | 2011-08-05 | Analyzing and reading device and method |
PCT/CN2011/078967 WO2013020307A1 (en) | 2011-08-05 | 2011-08-26 | Analysis and reading device and analysis and reading method |
Publications (3)
Publication Number | Publication Date |
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EP2587249A1 EP2587249A1 (en) | 2013-05-01 |
EP2587249A4 EP2587249A4 (en) | 2015-03-25 |
EP2587249B1 true EP2587249B1 (en) | 2020-07-22 |
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EP11867578.4A Active EP2587249B1 (en) | 2011-08-05 | 2011-08-26 | Analysis and reading device and analysis and reading method |
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EP (1) | EP2587249B1 (en) |
CN (1) | CN102323215A (en) |
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- 2011-08-05 CN CN201110223580A patent/CN102323215A/en active Pending
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US20130183199A1 (en) | 2013-07-18 |
CN102323215A (en) | 2012-01-18 |
US9097665B2 (en) | 2015-08-04 |
WO2013020307A1 (en) | 2013-02-14 |
EP2587249A1 (en) | 2013-05-01 |
EP2587249A4 (en) | 2015-03-25 |
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