EP2522427B1 - Micro-fluid device and method for manufacturing the same - Google Patents
Micro-fluid device and method for manufacturing the same Download PDFInfo
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- EP2522427B1 EP2522427B1 EP12179918.3A EP12179918A EP2522427B1 EP 2522427 B1 EP2522427 B1 EP 2522427B1 EP 12179918 A EP12179918 A EP 12179918A EP 2522427 B1 EP2522427 B1 EP 2522427B1
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- film
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- channel
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Definitions
- the present invention relates to microfluidic devices and methods of making same.
- Microfluidic devices are used in many fields of technology, such as e.g. for diagnostic applications.
- Microfluidic systems often consist, among other components, of one part, which is manufactured for example by means of injection molding. In this part, for example, there are channels or reservoirs.
- injection molding technology has the advantage that very large quantities can be manufactured here at low cost. However, this is associated with high initial costs, so that a later design change is no longer readily possible.
- a portion of such a microfluidic system may include a portion defined with its channel and reservoir system. It would be desirable to design such a system so that it would be customizable to the particular application or combination of sensors. The manufacturer costs should also remain low.
- the US 2002 / 0187072A1 refers to multilayer microfluidic splitters.
- a common fluid inlet fluidly communicates with a branching channel network, which evenly distributes a fluid flow to a plurality of outlets.
- Uniform splitting is provided by maintaining substantially equal fluidic impedance across all branch channels.
- Substantially equal fluidic impedance may be provided by maintaining a substantially equal flow path length between the common inlet and each of the outlets.
- the WO 02/083310 A2 refers to microfluidic devices capable of measuring fluid flow. Devices and methods for stem branch measurement of fluid stoppers in which one or more branch channels of a defined volume of mass fluid are measured are described. In addition, measuring at least one separate plug is described by selecting the delivery conditions of a first and a second fluid to a microfluidic channel.
- the first fluid may be a liquid and the second fluid may be a gas. Reduced area channel segments are provided to assist in measuring one or more separate fluid plugs.
- a microfluidic volume is measured by filling a microfluidic chamber with fluid, sealing an inlet channel, and then extracting the fluid.
- the document EP 1 205 670 A2 provides a distribution plate for liquids and / or gases, which consists of at least one layer, wherein in the layer or layers, a plurality of first elongated channels having a first geometric configuration formed in a first direction substantially, and also a plurality of second elongated channels is formed with a second geometric configuration substantially in a second direction.
- Such layers in particular if they have very small dimensions and accordingly miniaturized trained channels have been prefabricated in a simple manner and cost-saving, for example made of plastic by means of molding or injection molding in large numbers and stored. If necessary, in such a layer in a simple manner by means of suitable holes in spaces between juxtaposed channels at least a first and second channel are connected to each other, with appropriate requirements can be considered by the proper use of the distributor plate in an equally simple manner.
- the US 2002/0112961 A1 refers to multilayer microfluidic devices with folded channels and densely positioned microfluidic structures. Desired microfluidic structures which, when cut in a single device layer, would be subject to deformation may be formed from multiple non-deforming layers. Channel segments of each geometry, defined in separate layers, communicate to form continuous flow paths, which in turn form the desired microfluidic structures. Any number of device layers can be used to fabricate the microfluidic structures as desired.
- the US 2005/0266582 shows a microfluidic system for performing chemical or biological or biochemical reactions.
- the US 2002/0187074 shows a modular microfluidic system for performing fluidic operations, such as filtering, regulating, pressure adjusting, mixing, measuring, reacting, heating or cooling.
- the US 2008/0262213 shows methods and systems for editing polynucleotides.
- the object of the present invention is thus to provide a microfluidic device and a method for producing such microfluidic devices, so that low production costs can be achieved even with a high degree of design flexibility or a better relationship between production costs on the one hand and design flexibility on the other hand is achieved.
- microfluidic device according to claim 1 and a method of manufacturing according to claim 11.
- One finding of the present invention is that it has recognized that a foil having an opening therein can be used inexpensively for at least one component in which channel structures are formed that at least partially form a respective component surface of the at least one component are open to individualize to a respective one of a plurality of channel structure combinations.
- the manufacturing costs for the microfluidic devices can thus be kept low, since a plurality of such at least one component, which are identical to one another, can be used to produce different microfluidic devices which differ in the connection combination of the channel structures.
- a self-adhesive film can be used as the film, which makes the process of assembling the microfluidic device very simple
- the channel structures are also formed in a (common) microfluidic device so as to at least partially open in the component surface of the one component, wherein the film covers the component surface of this component such that a first and a second channel structure extend laterally along the component Component surface and within the opening in the film leading path interconnected While a third channel structure is not adjacent to the opening and is at least partially closed by the film on the component surface.
- a different microfluid device could be formed by a film covering the component surface of another identical component such that, for example, the third channel structure with one of the first or the second channel structure over a laterally along the component surface and within the opening of the last-mentioned film path connected to each other. Both microfluidic devices thus differ in the connection of the channel structures, although an identically shaped component is the basis.
- the production costs are therefore lower, in particular because the underlying at least one component can be manufactured in large quantities in injection molding.
- the opening on the side opposite the component is closed by a porous membrane, so that gas such as e.g. Air introduced when introducing liquids, e.g. Analytes or samples in which channel structures is displaced, can escape through the porous membrane, although the liquids are safely retained in the fluid structures.
- gas such as e.g. Air introduced when introducing liquids, e.g. Analytes or samples in which channel structures is displaced, can escape through the porous membrane, although the liquids are safely retained in the fluid structures.
- Fig. 1 shows the top view of a comparative example of a component with a channel structure.
- the component is, for example, an injection molded part and, as in the embodiments described below, the reference numeral 10 is used for the component.
- Fig. 1 shows the top view of a component surface 12 of the component 10.
- the outer shape of the component 10 is substantially cuboid but other shapes would also be conceivable, such as parallelepiped, cylindrical or the like.
- the component surface 12 as shown in FIG Fig. 1 is visible to one of the main sides of the component 10, and is also planar, but simply curved component surfaces would also be conceivable, for example.
- the embodiments described below will not be discussed in more detail, but the just made statements with respect to the component 10 also apply to the embodiments described below.
- a channel structure is formed in the component 10. It includes channels 14 and chambers 16a, 16b, 16c and 16d, of which the chambers 16b and 16d may serve as sensor sites, for example, where different sensors can perform different measurements in the respective chamber, while the chambers 16a and 16c, for example may be reservoirs or sources of fluids, such as analytes or samples.
- the channels 14 and 16a-16d are formed in the surface of the component in the form of recesses, with a cover component covering these recesses on the component surface 12, as a result of which the inside in FIG Fig. 1 formed fluid structure, according to which the channels 14 are branched into several sections.
- a channel section 14a-14d leads from a respective chamber 16a-16d to a common connecting section 14e.
- the section 14e connects nodes at which the sections 14a, 14c and 14e or 14b, 14d and 14e meet.
- Fig. 2a to 2d show the four possible combinations, according to which one of the reservoirs 16a and 16c is connected to one of the sensor sites 16b and 16d. As can be seen, only three of the channel sections would actually be needed. To realize the four different variants Fig. 2a - 2d Thus, either four different components 10 would have to be produced, or else the idea on which the exemplary embodiments described below are based is used.
- the channels located in the same, no direct connection by means of channels to the flexible chambers for the sensor sites or sources. Rather, the connection, so the channel or channels, is interrupted at one or more points.
- the injection molded part is then provided with a foil which has one or more openings or recesses which (in each case) form a kind of bridge between the separate channel structures, such as channel ends thereof, at the interruptions.
- Fig. 3 shows an embodiment of a microfluidic device according to an embodiment of the present invention.
- the microfluidic device of Fig. 3 is generally indicated with 20. It comprises a component 22, in which or in which channel structures 24a, 24b and 24c are formed which are at least partially open to a component surface 26 of the component 22.
- the component 22 may be an injection molded part.
- the channel structures are completely open to the component surface 26 of the component, ie they are formed in the form of depressions or recesses in the component surface 26.
- After assembly they are at least partially covered by a film 28 of the microfluidic device 20.
- the recesses 24a-24c include trenches 30a-30c and shafts 32a-32c which together with the film 28 form channels or chambers.
- the component 22 of Fig. 3 formed in the form of a substrate or as a flat cuboid and the channel structures 24a - 24c all open on one main side, namely the top of the component 22.
- the component 22 can in principle take any shape and of course as well the component surface 26.
- channel structures 24a-24c are laterally spaced from each other. This means that the channel structures 24a-24c have no fluidic connection to one another within the component 22.
- the fluidic connection between a subset of the channel structures 24a-24c is first produced by the film 28, as will be discussed in more detail below, wherein the subset each of the set of channel structures 24a-24c may correspond.
- Fig. 3 there are four possibilities for channel structure connection combinations.
- the component 22 can be an injection-molded part. It can thus be produced inexpensively in large quantities. Preferably, the component 22 is inherently stable and requires no further carrier. A flexible design would also be possible. Exemplary materials for component 22 include polycarbonate (PC), polymethylmethacrylate (PMMA), cycloolefin polymer (COP), and cycloolefin copolymer (COC).
- PC polycarbonate
- PMMA polymethylmethacrylate
- COP cycloolefin polymer
- COC cycloolefin copolymer
- the film is preferably designed flexibly.
- Material and film thickness or thickness may vary.
- the film thickness is less than or equal to 1 mm or even less than or equal to 0.5 mm.
- the material of the film 28 may be plastic, but other materials such as e.g. Metal.
- the film 28 comprises an opening 34, ie a recess which extends over the entire thickness of the film 28, ie from a front side of the film 28 to a rear side thereof.
- the film 28 is not yet shown in the assembled state.
- the film 28 In the assembled state, the film 28 is on the component surface 26, as indicated by dotted lines 36.
- the opening 34 connects the channel structures 24a and 24c. As indicated by dashed lines 38, it accomplishes this by providing in the assembled state, the channels 30a and 30c covered.
- the channel structure 24b is closed by the film 28 on the component surface 26, so that it is not in particular fluidly connected via the opening 34 with the other two channel structures 24a and 24b.
- the opening 34 in the film 28 thus implements one of the four channel structure connection combinations already mentioned above, wherein Fig. 7 another film 28 'is shown with a different opening 34', which leads to another of the four possible combinations in which the channel structures 24a and 24b are interconnected by the opening 34 'in the assembled state covering the trenches 30a and 30b but laterally separated from the channel structure 24c. It is readily apparent how films could look for the other two possible combinations.
- Fig. 3 What in Fig. 3 is not shown, that the opening 34 in the film 28 and the therein of the channel structure 24a to the channel structure 24c and vice versa leading path on a side facing away from the component surface 26 is closed with a lid.
- the lid can, as it later regarding the Fig. 4 to 5b can be a porous membrane, but can also, as it regards Fig. 6 is shown, another component, ie, a further injection molded part, in which possibly even one or more or more channel structures are formed.
- the film is glued to the component surface 26, for example. It is advantageous if the component surface 26, as in Fig. 3 shown, flat or at least only slightly curved so that no wrinkles form during application. Corners or edges could also be present in the surface.
- the film 28 may in particular be a self-adhesive film. So if the microfluidic device of Fig. 3 is produced, then it is sufficient for joining of the component and the film to apply the film 28 on the component surface 26, as for example, by rolling and / or pressing, with the self-adhesive side facing the component surface.
- the self-adhesive film is, for example, an adhesive tape.
- the film 28 may also be a self-adhesive film on both sides, such as adhesive tape provided on both sides with an adhesive layer.
- An otherwise gluing the lid on the film 28 is also possible.
- the components could also be clamped with a clamping device such as an extra frame which presses the film 28 against the surface 26.
- An adhesive or an adhesive layer between the components could also be an attachment by means of melting, such as the film material 28, to the surface 26, are used.
- the adhesive which adjoins the channel structures and, in particular, the fluid located therein may be chosen such that the abutment for the respective application is not critical. The same applies regardless of the presence or absence of the self-adhesive property also for the material of the film.
- Fig. 3 in many respects is merely exemplary with respect to the design of the channel structures 24a-24c in the component 22. It has already been pointed out that the channel structures 24a-24c are merely exemplary in FIG Fig. 3 have only depressions. Rather, the channel structures 24a-24c could also be partially buried formed in the interior of the component 22, ie parts that are not first closed by the film 28 on the component surface 26. Furthermore, the channel structures 24a - 24c may also have holes or passages to an opposite side of the component 22.
- Such a passage is exemplified by a dotted line in FIG Fig. 3 indicated at 40 in the bottom of the duct 32a.
- This passage could, for example, serve as an outlet or inlet for a liquid if the channel structure 24a is to serve as a source of fluids or as an outlet.
- the opening 40 could also be provided so that a sensor attachable to the underside of the component 22 can come into contact with the liquid in the chamber 32a to make a sensor measurement, such as electrochemical, potentiometric, amperometric, optical Measurement, a gravimetric or the like.
- a sensor could already be installed prior to delivery of the microfluidic device 20 in the course of production or only be mounted after delivery to the customer.
- the microfluidic device 20 it is possible for the microfluidic device 20 to be a disposable product, whereas the sensor is used multiple times.
- the number of channel structures here is only three by way of example and may be more.
- Fig. 4 now shows a side sectional view of the microfluidic device of Fig. 3 , As can be seen, the opening 34 in the film 28 is closed by a lid on a side opposite the component surface 26, the lid being a porous membrane 42.
- the porous membrane 34 allows outgassing of excess air.
- the porous membrane 42 may in particular consist of a material or have a surface which faces the opening 34 in the film 28, which with Water forms a contact angle greater than 90 ° or is water-repellent. Of course, the material could also be formed to additionally or alternatively form a contact angle greater than 90 ° with other materials.
- the film 28 is made thin.
- the porous membrane in addition to the reduction in size, it offers an advantage when the film is made thinner: due to the reduced in this region in contrast to the channel structures flow cross-section in the region between the membrane 42 and surface 26, locally increases the pressure , which promotes the outgassing through the membrane 42.
- the flow area of the flow path in the area of the opening 34 is smaller than the average cross section of the channels of the channel structures (i.e., excluding the chambers), e.g. less than 80% or even less than 50% of the latter.
- Fig. 3 shows that the film 28, the surface 26 over the whole or a part covered so that the channel structures 24a - 24c, as far as the opening to the surface 26 is concerned, are completely covered, this is not absolutely necessary.
- the porous membrane 42 can be over the entire surface of the film 28 formed across or be attached to her, but it is of course also possible that it protrudes only slightly beyond the edge of the opening 34.
- the film 20 may also have further openings 44.
- aperture 44 is aligned with chamber 32c in the assembled condition.
- Fig. 5a It is shown that the resulting upward opening can be used as an example to displace liquid contained in the chamber 32c therefrom. As in the example of Fig. 5a can be seen, this is a deformable membrane 46 provided to cover the opening 44 on a side facing away from the surface 26 of the film 28.
- An actuator 48 is provided to urge the membrane into the opening 44 and the chamber 32c, respectively.
- the actuator could be configured differently, such as by means of a piezoelectric element or the like, is in Fig.
- a variant is shown, according to which the actuator 48 is mounted on a side facing away from the component 22 to the film 28 or the deformable membrane 46, so that on a side facing away from the opening 44 of the deformable membrane 46, a sealed chamber 50 is formed containing a substance, such as water, which is chemically converted from a liquid to a gaseous state by electrolysis by means of electrodes 52 located in the chamber 50, whereby the resulting density reduction and expansion is a force on the deformable membrane 46 exerts, which then bulges into the opening 44 and chamber 32 c inside and displaces liquid there.
- the deformable membrane 46 is, for example, a flexible membrane that tends to return to its original state. As it is in Fig.
- the actuator may be formed by a multilayer arrangement of multiple layers 54a and 54b, such as a multilayer board, such as a spacer layer 54b having a recess for the chamber 50 and a layer 54a having the electrodes 52 the spacer layer 54b is located between the layer 54a and the substrate 22.
- a multilayer board such as a spacer layer 54b having a recess for the chamber 50 and a layer 54a having the electrodes 52 the spacer layer 54b is located between the layer 54a and the substrate 22.
- Fig. 3 is indicated with a dashed line 56 that it is possible that the flexible membrane 46 and / or the multilayer assembly 54 is laterally located on one side only, whereas the other side of the line 56 is covered by the porous membrane 42.
- Fig. 5b shows one to Fig. 5a alternative example.
- the film 28 already has a sufficiently high ductility to be pressed by the actuator 48 in the direction of the chamber 32c in order to displace the liquid content located in the chamber 32c.
- the opening 44 may be missing and the actuator 48 may be mounted directly on the film 28 on a side facing away from the component 22 thereof.
- Fig. 6 shows an unclaimed alternative already mentioned above Fig. 3 , according to which a further component 56 is used as cover.
- Fig. 6 shows Fig. 6 in that it is possible that the channel structures 24a-24c of a microfluidic device according to examples are not all provided in a single component 22, but that they are formed distributed in a plurality of components.
- Fig. 6 shows by way of example therefore a modified component 22 ', which differs from the one Fig. 3 characterized in that the channel structure 24c in the component 22'mVM. Rather, this channel structure 24c is in the assembled state as shown in FIG Fig.
- FIG. 6 is shown, mirror image of its original position in the component 22 'relative to the plane of the film 28 formed in the component 56, in a component surface 58 thereof, with which the component 56 to the component 22' opposite side of the film 28 added is.
- the component 56 has, for example, the same dimensions as the component 42 ', that is, for example, also substrate-like or cuboid.
- the opening 34 in the film 28 thus connects channel structures in different components 22 'and 56 respectively, namely the channel 30a with the channel 30c in the component 56.
- FIG Fig. 6 Similar advantages with respect to the channel structure connection combinations can be achieved, as is the case in the exemplary embodiment of FIG Fig. 3 and 7, respectively, but with reference to FIG Fig. 4 has been described, the embodiment according to Fig. 3 offers the possibility of using a porous membrane, with the associated benefits in terms of outgassing, etc.
- the channel structures 24a-24c which were to be combined in a combination manner, each had a trench 30a-30c which, at least over a section 60 (FIG. Fig. 7 ) parallel to one another, ie such that at least one channel runs parallel to another channel.
- the individual channels project more or less into the section 60 from the two sides along the channel propagation direction 62.
- This configuration allows elongated apertures in the film 28 having a longitudinal direction 64 transverse to the straight span direction 62 to more or less selectively interconnect the channel structures. The location of these openings 34 in the directions 62 and 64 and the length of the direction 64 of these openings then determines which channel structures are interconnected.
- FIG. 8 An embodiment of a component 22 having five channel structures 24a-24e, each also having a trench 30a-30e extending across a portion 60 along the component surface 26, is parallel to one another, such that a plurality of channel structure combination options exist by forming an aperture 34 of the film over the component surface 26 is varied with a longitudinal direction 64 transverse to the trench extension direction 62 within the region 60 in position and length of the opening 34, as referenced in FIG Fig. 7 has been described.
- Fig. 9a - 9d show four different combinations. Black arrows in the figures indicate channel structures which are interconnected via the respective opening 34. White arrows were used for channel structures that are kept separate from the connected channel structures.
- trenches 30c and 30d it may be useful to collinearly guide some of the trenches of the channel structures, here trenches 30c and 30d, from opposite directions into region 60, with a gap 66 therebetween, with which they extend in the extension direction 62 from one another are spaced, wherein the gap 66 in the direction 62 is sufficiently large, for example, to accommodate the width of one of the elongated openings 34.
- Fig. 9a - 9d Now show different layers of the opening 34 in the film 28. The openings 34 of Fig. 9a - 9d always connects three of the channel structures together, as shown in the figures.
- Fig. 10 shows a to the embodiment of Fig. 8 - 9d similar embodiment of a microfluidic device. While in Fig. 10 the state is shown in which the film and a porous membrane are not mounted as a lid, show the Fig. 11 and 12 each state with foil, but without membrane or both.
- Fig. 13 shows a sectional view in which the opening 34 can be seen in the film.
- the embodiment of Fig. 10 - 13 corresponds to the embodiment of the Fig. 3 in the example / execution according to Fig. 4 and Fig. 5a and thus also shows an example such as a restriction of the lateral expansion area for the porous membrane 42 as indicated by the dashed line 56 in FIG Fig. 3 may also have been visualized.
- Fig. 10 shows a to the embodiment of Fig. 8 - 9d similar embodiment of a microfluidic device. While in Fig. 10 the state is shown in which the film and a porous membrane are not mounted as a lid, show the Fig. 11 and
- the film 28 extends over the entire surface on the upper side 26 of the component 22.
- the porous membrane 42 extends only laterally in the interior of the recess in the multilayer arrangement of the actuator 48.
- the actual actuator locations of the actuator 46 are in Fig. 10 - 13 not shown, but can for example like in Fig. 5a be designed shown.
- a one-sided adhesive film can be used as the film, and the use of a double-sided adhesive film can be particularly advantageous.
- a lid may be provided to close a channel open at this point.
- This cover can also be designed in the form of a foil, as has been described above.
- the lid can be limited laterally to the recess. He closes the recess from above.
- the lid does not have to be completely closed here.
- the lid may be formed by a porous membrane. This allows escape of possibly unwanted and possibly present in the duct system gas bubbles.
- the porous membrane may also be formed of a material which is not wetted by the liquid. If the liquid is a water-based liquid, a membrane with a surface or a material with a low surface energy is particularly suitable here. Examples include fluoropolymers, such as PTFE, PVDF, etc.
- the easy combinability of configurable fluidic connections and bubble trap represents a further advantage of some embodiments described above, since this only three parts are needed, namely the fixed component with channel system or reservoirs, the structured film and the cover membrane.
- a liquid source which is not required to be separated.
- a fixed microfluidic part such as e.g. one of the components 22 of the embodiments described above, contains a plurality of reservoirs, for a particular application, however, only a part thereof is needed. If, in such a case, all reservoirs were connected to each other by channels, then liquid could compress the air in these reservoirs and thus flow in the direction of these empty (because not required) reservoirs.
- the problem can be solved in that the reservoirs are just not directly connected to the channel system, but, as described above, are first connected to each other via a "bridge" in the form of a recess with a film.
- a "bridge" in the form of a recess with a film.
- the chambers 32a, 32d and 32e are reservoirs and the chambers 32c and 32b are sensor sites, i. H. Places where sensors are positioned or can be positioned.
- the above-mentioned reservoirs can also be provided with pumps.
- Such pumps can be operated by electrolysis, as previously described.
- the electrolysis thereby generates a gas, namely in the above-mentioned chamber 50, and deforms a membrane, namely the deformable membrane 46, which is adjacent to the respective reservoir is located.
- the membrane can then warp into this reservoir and displace the fluid contained therein.
- the liquid pumped from a reservoir by means of the electrolysis pump could be introduced into the empty (instead of outward / waste) container. air-shrouded) reservoir flow.
- the only alternative to the above "separation" of the reservoir by means of suitable placement and design of the film according to the above embodiments would be only in the filling of the unused reservoirs, but this meant an additional material and manufacturing costs.
- the membrane 46 which in this case is preferably not a porous membrane, but rather preferably a membrane which deforms plastically, for example, can be temporarily or permanently connected to two or more channels upon impact with a pressure in the direction of the fixed part or component 22 interrupt.
- Fig. 14 shows such an alternative.
- the deformable membrane 46 is used, above which in turn is an actuator 48.
- the cross-section of the lateral path 70 through the opening 34 in the film 28 may be at least reduced or the path interrupted. Narrowing in the cross-section of the path 70 may often be sufficient.
- Fig. 15 shows a further alternative to the embodiment of Fig. 14 ,
- the component 22 "to the component of Fig. 3 formed differently, namely in that the area of the surface 26 between the channels 30a and 30c is lowered in the region of the opening 34 by a depth which is smaller than a depth of the trenches 30a - 30c, so that the flow resistance can be adjusted, which results when the membrane 46 is pressed and when the membrane 46 is not depressed.
- an increase may be present.
- FIGS. 14 and 15 achieve a valve effect.
- Such a step may, for example, be done after filling a reservoir to close it.
- the membrane 46 again dissolves from the component 22 or 22 "and the liquid can leave the reservoir in the channel system by means of the path 70 in the channel system.
- the application of the membrane 46 with a pressure in the direction of the component 22 can also be used as an active valve, if, for example, directly or indirectly the Pressure of a gas pressure generated by the electrolysis is.
- the interruption between the channels or the channel or the reservoir need not be complete, but may also be formed as a recess, which is, however, preferably shallower than the subsequent channel, as with reference to FIG Fig. 15 has been shown, ie by means of a flat portion in a channel of the channel structures.
- Such a depression does not necessarily have to be present between separate channel structures in the sense of the channel structures 24a-24e of the preceding exemplary embodiments.
- Such a depression in the trenches can also be present in the above-mentioned trenches 30a-30c within a single channel structure in order, as mentioned, to control the flow from a corresponding reservoir or into a corresponding reservoir.
- microfluidic devices in which it was possible to form different microfluidic devices based on a fixed microfluidic part that is identical for all. It was possible, for example by two sensors and two fluid sources each one to connect via a bridge with the channel system and another liquid source. This was, for example, in the embodiments after Fig. 9a and 9d the case where unfilled arrows indicate that no liquid can flow here.
- the above embodiments also show implementation variants, with a porous membrane as a bubble trap. Valves for closing, z. B. a reservoir may be present, as has been described above.
- the above embodiments thus also describe a microfluidic system which has at least one part with fixed channel structures, wherein at least two channel structures in the stationary part initially have no connection to one another, the connection is instead produced by a foil which at least partially covers the channel structures, an opening connecting at least two of the unconnected channel structures in the stationary part.
- Two channel structures not connected to one another in the stationary part can each lead to an alternatively populated position.
- the microfluidic system may be designed such that two channel structures which are not connected to one another in the stationary part come from a different fluid source or reservoir.
- non-interconnected channel structures there are at least four non-interconnected channel structures in the stationary part, three each of which can be connected by a recess in a foil to select one of two alternative sensor regions or two alternative liquid sources and to another liquid source connect.
- at least five non-interconnected channel structures are present in the fixed part, of which three by means of a Recess may be connected in a film, wherein a solid fluid source is connected to one of two alternative sensor areas and one of two alternative fluid sources.
- the film may be an adhesive tape, wherein the adhesive tape may in turn be an adhesive tape provided with an adhesive layer on both sides.
- the recess in the film is closed with a lid. This lid has a porous membrane.
- the material of this membrane may be made of a material or be coated with selbigem that forms a contact angle greater than 90 ° with the channel system liquid to be transported.
- the porous membrane may be made of a water-repellent material, wherein the water-repellent material may also be a fluorine-containing polymer.
- a membrane On the side facing away from the fixed part of the film, a membrane may be located, which can be at least partially pressed into the recess of the film by applying pressure. In this case, the necessary pressure for the deformation by electrolysis of water or at least partially water-containing liquid can be caused.
- the above exemplary embodiments have shown a microfluid device with at least one component 22, in which or in which channel structures 24a are formed, which are at least partially open to a respective component surface 26 of the at least one component 22; and a film 28 having an opening 24; 34 ', via which at least a first and a second of the channel structures 24a, 24c; 24a, 24b are interconnected, and the at least one third of the channel structures at 24b; 24c of the respective component surface 26 at least partially closes, so that it does not over the opening 34; 34 'is connected to the first and second channel structure.
- the first to third channel structure can be formed in the same component and at least partially open in the component surface thereof, the film covering the component surface of the same component such that the first and second channel structure extend laterally along the component surface of the same component and inside the opening in the film leading path are connected to each other, while the third channel structure is not adjacent to the opening.
- the path leading within the opening 24 in the film can be closed on one side of the component 26 facing away from the same component 22 with a lid or with another of the at least one component.
- the path leading through the opening in the film on a side remote from the component surface of the same component is closed by a deformable membrane as a cover.
- the path leading through the opening 34 in the film 28 is on a side facing away from the component surface 26 of the same component 22 a porous membrane 42 is closed as a lid.
- a porous membrane 42 is closed as a lid.
- the path leading through the opening in the film on a side facing away from the component surface of the same component can be closed with a deformable membrane as a lid.
- the microfluid device may further include an actuator for urging the deformable membrane into the opening.
- the first to third channel structures 24a may have depressions in the component surface of the same component, which are at least partially covered by the film.
- the recesses may include trenches 30a and / or wells 32a to form channels or chambers together with the foil.
- the film is deformable, and the microfluid device further comprises an actuator for pressing the film into a recess of the at least one component on the respective component surface in which the recess is formed, wherein the recess is part of the channel structures.
- the film 28 may be a self-adhesive film.
- the film 28 may also be a self-adhesive film on both sides.
- An actuator 48 may be configured to generate a force necessary for pressing by electrolysis of water or at least partially water-containing liquid.
Description
Die vorliegende Erfindung bezieht sich auf Mikrofluidvorrichtungen und Verfahren zum Herstellen derselben.The present invention relates to microfluidic devices and methods of making same.
Mikrofluidvorrichtungen bzw. mikrofluidische Systeme werden in vielen Bereichen der Technik verwendet, wie z.B. für diagnostische Anwendungen. Mikrofluidische Systeme bestehen häufig neben anderen Komponenten aus einem Teil, welches beispielsweise mittels Spitzguss gefertigt ist. In diesem Teil befinden sich beispielsweise Kanäle oder Reservoire. Die Fertigung in Spritzgusstechnologie besitzt den Vorteil, dass hier sehr große Stückzahlen zu geringen Kosten gefertigt werden können. Allerdings ist dies mit hohen Initialkosten verbunden, so dass ein späterer Designwechsel nicht mehr ohne weiteres möglich ist.Microfluidic devices are used in many fields of technology, such as e.g. for diagnostic applications. Microfluidic systems often consist, among other components, of one part, which is manufactured for example by means of injection molding. In this part, for example, there are channels or reservoirs. The production in injection molding technology has the advantage that very large quantities can be manufactured here at low cost. However, this is associated with high initial costs, so that a later design change is no longer readily possible.
Häufig ist es jedoch einerseits notwendig, wie z.B. aus wirtschaftlichen Gründen oder weil in der dafür eingesetzten Technologie bessere Oberflächenqualitäten erreicht werden können, wie beschrieben, ein in seinen Strukturen nicht mehr veränderliches Teil zu verwenden, gleichzeitig jedoch eine Flexibilität bezüglich des späteren Flüssigkeitstransportes zu gewährleisten. Beispielsweise kann es sinnvoll sein, ein einziges Spritzgussteil zu verwenden, bei dem wahlweise Sensoren miteinander kombiniert werden können, die sich in ihren Abmessungen und ihrer Position am Spritzgussteil unterscheiden. Auch kann es sich als notwendig erweisen, je nach Einsatzzweck unterschiedliche "Quellen" für eine Flüssigkeit vorzusehen. In diesem Fall möchte man trotz des feststehenden Teils, wie z.B. Spritzgussteils, anhand anderer konstruktiver Elemente am (Mikro)-Fluidsystem bestimmte Kanalabschnitte abtrennen und andere hinzu "schalten".Often, however, it is necessary on the one hand, such as for economic reasons or because better surface qualities can be achieved in the technology used for this purpose, as described, to use a part which is no longer changeable in its structures, but at the same time to ensure flexibility with regard to the subsequent liquid transport. For example, it may be useful to use a single injection molded part, in which optionally sensors can be combined with each other, which differ in their dimensions and their position on the injection molded part. It may also prove necessary to provide different "sources" for a liquid depending on the application. In this case, despite the fixed part, e.g. Injection molded part, on the basis of other constructive elements on the (micro) -luidsystem seperate certain channel sections and other "switch".
Wie erwähnt, kann ein Teil eines solchen mikrofluidischen Systems ein Teil enthalten, welches mit seinem Kanal und Reservoirsystem festgelegt ist. Wünschenswert wäre es, ein solches System so zu gestalten, dass es auf die jeweilige Applikation bzw. Kombination von Sensoren individualisierbar wäre. Die Herstellerkosten sollen ebenfalls niedrig bleiben.As noted, a portion of such a microfluidic system may include a portion defined with its channel and reservoir system. It would be desirable to design such a system so that it would be customizable to the particular application or combination of sensors. The manufacturer costs should also remain low.
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Die Aufgabe der vorliegenden Erfindung besteht folglich darin, eine Mikrofluidvorrichtung sowie ein Verfahren zum Herstellen solcher Mikrofluidvorrichtungen zu schaffen, so dass niedrige Herstellungskosten auch bei einem hohen Grad an Designflexibilität erzielbar sind bzw. ein besseres Verhältnis zwischen Herstellungskosten auf der einen und Designflexibilität auf der anderen Seite erzielt wird.The object of the present invention is thus to provide a microfluidic device and a method for producing such microfluidic devices, so that low production costs can be achieved even with a high degree of design flexibility or a better relationship between production costs on the one hand and design flexibility on the other hand is achieved.
Diese Aufgabe wird durch eine Mikrofluidvorrichtung gemäß Anspruch 1 und ein Verfahren zum Herstellen gemäß Anspruch 11 gelöst.This object is achieved by a microfluidic device according to
Eine Erkenntnis der vorliegenden Erfindung besteht darin, erkannt zu haben, dass eine Folie mit einer Öffnung darin dazu verwendet werden kann, preisgünstig zumindest ein Bauteil, in dem bzw. in denen Kanalstrukturen gebildet sind, die zumindest teilweise zu einer jeweiligen Bauteiloberfläche des zumindest einen Bauteils offen sind, zu einer jeweiligen aus einer Mehrzahl von Kanalstrukturkombinationen zu individualisieren. Die Herstellungskosten für die Mikrofluidvorrichtungen können somit gering gehalten werden, da eine Vielzahl von einem solchen zumindest einen Bauteil, die zueinander identisch sind, verwendet werden können, um unterschiedliche Mikrofluidvorrichtungen herzustellen, die sich in der Verbindungskombination der Kanalstrukturen unterscheiden.One finding of the present invention is that it has recognized that a foil having an opening therein can be used inexpensively for at least one component in which channel structures are formed that at least partially form a respective component surface of the at least one component are open to individualize to a respective one of a plurality of channel structure combinations. The manufacturing costs for the microfluidic devices can thus be kept low, since a plurality of such at least one component, which are identical to one another, can be used to produce different microfluidic devices which differ in the connection combination of the channel structures.
Gemäß Ausführungsbeispielen kann als Folie eine selbstklebende Folie verwendet werden, was den Vorgang des Zusammenfügens der Mikrofluidvorrichtung sehr einfach gestalten lässtAccording to embodiments, a self-adhesive film can be used as the film, which makes the process of assembling the microfluidic device very simple
Gemäß Ausführungsbeispielen sind die Kanalstrukturen zudem in einer (gemeinsamen) Mikrofluidvorrichtung gebildet, um sich in der Bauteiloberfläche des einen Bauteils jeweils zumindest teilweise zu öffnen, wobei die Folie die Bauteiloberfläche dieses Bauteils so bedeckt, dass eine erste und eine zweite Kanalstruktur über eine lateral entlang der Bauteiloberfläche und innerhalb der Öffnung in der Folie führenden Pfad miteinander verbunden sind, während eine dritte Kanalstruktur nicht an die Öffnung angrenzt und von der Folie an der Bauteiloberfläche zumindest teilweise verschlossen wird. Mit dem gleichen Bauteil könnte eine unterschiedliche Mikrofluidvorrichtung gebildet werden, indem eine Folie die Bauteiloberfläche eines weiteren identischen Bauteils so bedeckt, dass beispielsweise die dritte Kanalstruktur mit einer der ersten oder der zweiten Kanalstruktur über eine lateral entlang der Bauteiloberfläche und innerhalb der Öffnung letztgenannter Folie führenden Pfads miteinander verbunden sind. Beide Mikrofluidvorrichtungen unterscheiden sich also in der Verbindung der Kanalstrukturen, obwohl ein identisch geformtes Bauteil zugrunde liegt.According to exemplary embodiments, the channel structures are also formed in a (common) microfluidic device so as to at least partially open in the component surface of the one component, wherein the film covers the component surface of this component such that a first and a second channel structure extend laterally along the component Component surface and within the opening in the film leading path interconnected While a third channel structure is not adjacent to the opening and is at least partially closed by the film on the component surface. With the same component, a different microfluid device could be formed by a film covering the component surface of another identical component such that, for example, the third channel structure with one of the first or the second channel structure over a laterally along the component surface and within the opening of the last-mentioned film path connected to each other. Both microfluidic devices thus differ in the connection of the channel structures, although an identically shaped component is the basis.
Die Herstellungskosten sind also insbesondere auch deshalb senkbar, weil das zugrundeliegende zumindest eine Bauteil in großen Stückzahlen in Spritzguss gefertigt werden kann.The production costs are therefore lower, in particular because the underlying at least one component can be manufactured in large quantities in injection molding.
Unter den letztgenannten Ausführungsbeispielen mit lateralem Verbindungspfad zwischen Kanalstrukturen innerhalb der Öffnung in der Folie sind bei den Ausführungsbeispielen die Öffnung auf der dem Bauteil gegenüberliegenden Seite von einer porösen Membran verschlossen, so dass Gas, wie z.B. Luft, die beim Einbringen von Flüssigkeiten, wie z.B. Analyten oder Proben, in den Kanalstrukturen verdrängt wird, über die poröse Membran entweichen kann, wiewohl die Flüssigkeiten sicher in den Fluidstrukturen zurückgehalten werden.In the latter embodiments with lateral communication path between channel structures within the opening in the foil, in the embodiments, the opening on the side opposite the component is closed by a porous membrane, so that gas such as e.g. Air introduced when introducing liquids, e.g. Analytes or samples in which channel structures is displaced, can escape through the porous membrane, although the liquids are safely retained in the fluid structures.
Weitere bevorzugte Ausgestaltungen sind Gegenstand der abhängigen Patentansprüche.Further preferred embodiments are the subject of the dependent claims.
Nachfolgend werden bezugnehmend auf die Zeichnungen bevorzugte Ausführungsbeispiele der vorliegenden Anmeldung detailliert beschrieben, worunter
- Fig. 1
- eine schematische Draufsicht auf ein Bauteil zeigt, wie es für eine Mikrofluidvorrichtung gemäß einem Vergleichsbeispiel verwendet werden könnte;
- Fig. 2a-d
- schematische Draufsichten auf das Bauteil von
Fig. 1 zur Veranschaulichung von vier verschiedenen exemplarischen Zielverbindungskombinationen zwischen den Kanalstrukturen und dem Bauteil; - Fig. 3
- eine Raumansicht einer Mikrofluidvorrichtung gemäß einem Ausführungsbeispiel mit noch nicht angefügter Folie und ohne Deckel;
- Fig. 4
- eine Seitenschnittansicht der Mikrofluidvorrichtung von
Fig. 3 entlang einer Schnittebene, die durch die Öffnung in der Folie verläuft; - Fig. 5a
- und 5b Seitenschnittansichten der Mikrofluidvorrichtung von
Fig. 3 entlang einer Schnittebene, die durch eine Kammer der Kanalstrukturen verläuft gemäß zweier unterschiedlicher Beispiele; - Fig. 6
- eine Seitenschnittansicht einer Mikrofluidvorrichtung, die durch die Öffnung in der Folie verläuft, gemäß einem zur
Fig. 3 alternativen Beispiel; - Fig. 7
- eine Raumansicht einer Mikrofluidvorrichtung mit einem Bauteil, das zu demjenigen von
Fig. 3 identisch ist, jedoch mit einer unterschiedlichen Folie zur Erzielung einer anderen Kanalstrukturverbindungskombination; - Fig. 8
- eine Draufsicht auf die Bauteiloberfläche eines Bauteils, das als Grundlage für verschiedene unterschiedliche Mikrofluidvorrichtungen dienen kann, gemäß einem Ausführungsbeispiel;
- Fig. 9a
- bis 9d Aussichten auf die Mikrofluidvorrichtung von
Fig. 8 mit eingezeichneter Öffnung der Folie, die die Bauteiloberfläche bedeckt, für vier unterschiedliche Kanalstrukturverbindungskombinationen gemäß einem Ausführungsbeispiel; - Fig. 10
- eine Teilraumansicht auf eine Mikrofluidvorrichtung gemäß einem Ausführungsbeispiel mit ausgeblendeter Folie und darüber befindlicher Membran als Deckel;
- Fig. 11
- eine Teilraumansicht auf die Mikrofluidvorrichtung von
Fig. 10 aus gleicher Perspektive, allerdings mit lediglich ausgeblendeter Membran und vorhandener Folie; - Fig. 12
- Teilraumansicht auf die Mikrofluidvorrichtung von
Fig. 10 undFig. 11 mit Folie und Membran; - Fig. 13
- Teilraumschnittansicht der Mikrofluidvorrichtung gemäß der
Fig. 10 , wobei die Schnittebene durch die Kanalstruktur verbindende Öffnung in der Folie verläuft;bis 12 - Fig. 14
- eine Seitenschnittansicht einer Mikrofluidvorrichtung entlang einer Schnittebene, die durch die Öffnung in der Folie verläuft, gemäß einem alternativen Ausführungsbeispiel; und
- Fig. 15
- eine Seitenschnittansicht einer Mikrofluidvorrichtung entlang einer Schnittebene, die durch einen flachen Abschnitt eines Kanals einer Kanalstruktur verläuft gemäß einem alternativen Ausführungsbeispiel.
- Fig. 1
- a schematic plan view of a component, as could be used for a microfluidic device according to a comparative example;
- Fig. 2a-d
- schematic plan views of the component of
Fig. 1 to illustrate four different exemplary target connection combinations between the channel structures and the component; - Fig. 3
- a perspective view of a microfluidic device according to an embodiment with not yet attached foil and without cover;
- Fig. 4
- a side sectional view of the microfluidic device of
Fig. 3 along a cutting plane passing through the opening in the film; - Fig. 5a
- and FIG. 5b are side sectional views of the microfluidic device of FIG
Fig. 3 along a sectional plane passing through a chamber of the channel structures according to two different examples; - Fig. 6
- a side sectional view of a microfluidic device, which passes through the opening in the film, according to a
Fig. 3 alternative example; - Fig. 7
- a space view of a microfluidic device with a component that to that of
Fig. 3 is identical, but with a different film to achieve a different channel structure combination; - Fig. 8
- a plan view of the component surface of a component, which may serve as a basis for various different microfluidic devices, according to an embodiment;
- Fig. 9a
- to 9d prospects for the microfluidic device of
Fig. 8 with a marked opening of the film which covers the component surface, for four different channel structure connection combinations according to an embodiment; - Fig. 10
- a partial space view of a microfluidic device according to an embodiment with hidden film and befindlichem membrane as a lid;
- Fig. 11
- a partial space view of the microfluidic device of
Fig. 10 from the same perspective, but with only blanked membrane and existing film; - Fig. 12
- Partial view of the microfluidic device of
Fig. 10 andFig. 11 with foil and membrane; - Fig. 13
- Subspace sectional view of the microfluidic device according to the
10 to 12 wherein the sectional plane extends through the channel structure connecting opening in the film; - Fig. 14
- a side sectional view of a microfluidic device along a cutting plane which passes through the opening in the film, according to an alternative embodiment; and
- Fig. 15
- a side sectional view of a microfluidic device along a sectional plane passing through a flat portion of a channel of a channel structure according to an alternative embodiment.
Bevor im Folgenden detaillierte Ausführungsbeispiele der vorliegenden Anmeldung beschrieben werden, wird darauf hingewiesen, dass einander gleiche oder entsprechende Elemente in diesen Figuren mit den gleichen Bezugszeichen versehen sind, und dass eine wiederholte Beschreibung dieser Elemente und ihrer Funktion unterbleibt. Vielmehr sollen die Ausführungen zu diesen Elementen zu vorhergehenden Figuren auch auf die nachfolgenden Figuren übertragbar sein, soweit nicht in der jeweiligen Figur eigens auf Unterschiede hingewiesen wird.Before describing detailed embodiments of the present application in the following, it is pointed out that the same or corresponding one another Elements in these figures are provided with the same reference numerals, and that a repeated description of these elements and their function is omitted. Rather, the remarks on these elements to previous figures should also be transferable to the following figures, unless specifically indicated in the respective figure differences.
In dem Fall von
Zum Verständnis der Prinzipien und Vorteile nachfolgend beschriebener Ausführungsbeispiele der vorliegenden Erfindung ist es nicht wesentlich wie das Bauteil 10 von
Aus verschiedenen Gründen kann es aber bei einigen Anwendungen wünschenswert sein, wenn einige der Kanalabschnitte 14a - 14d fehlten. Ein nicht benötigtes Reservoir kann beispielsweise dazu führen, dass Flüssigkeit, wie z.B. Analyt oder eine Probe, in Richtung dieses Reservoirs abzweigt. Dies könnte zu verschiedenen unerwünschten Nebeneffekten führen. Ähnliches gilt für die Sensorstätten 16b und 16d. In einigen Anwendungen mag es bevorzugt sein, wenn lediglich eine Sensorstätte von einem Reservoir erreicht wird. Die
In anderen Worten ausgedrückt, wäre es wünschenswert, im Laufe der Fertigung der Mikrofluidvorrichtung den Weg der Flüssigkeit in den Kanälen so näher zu bestimmen, dass beispielsweise die Flüssigkeit entweder nur zu dem einen Sensor 16b oder zu dem anderen Sensor 16d fließen kann, wobei gleiches für die Quellen 16a und 16c der Flüssigkeit gilt. Gemäß nachfolgend beschriebenen Ausführungsbeispielen besitzen im Vergleich zu dem Bauteil von
Wie es in
In dem Fall von
Bei dem Bauteil 22 kann es sich, wie im Vorhergehenden erwähnt, um ein Spritzgussteil handeln. Es kann damit kostengünstig in großen Stückzahlen hergestellt werden. Bevorzugterweise ist das Bauteil 22 in sich stabil und benötigt keinen weiteren Träger. Eine flexible Ausgestaltung wäre aber ebenfalls möglich. Beispielhafte Materialien für das Bauteil 22 sind Polycarbonat (PC), Polymetylmetacrylat (PMMA), Cycloolefinpolymer (COP) und Cycloolefin-Copolymer (COC).As mentioned above, the
Die Folie ist vorzugsweise flexibel ausgestaltet. Material und Folienstärke bzw. -dicke können variieren. Beispielsweise ist die Foliendicke kleiner oder gleich 1 mm oder sogar kleiner oder gleich 0,5 mm. Das Material der Folie 28 kann Kunststoff sein, wobei aber auch andere Materialien vorstellbar sind, wie z.B. Metall.The film is preferably designed flexibly. Material and film thickness or thickness may vary. For example, the film thickness is less than or equal to 1 mm or even less than or equal to 0.5 mm. The material of the
Die Folie 28 umfasst eine Öffnung 34, d.h. eine Aussparung, die sich über die gesamte Dicke der Folie 28 hinweg erstreckt, d.h. von einer Vorderseite der Folie 28 bis zu einer Rückseite derselben. In
Was in
Die Folie ist beispielsweise auf die Bauteiloberfläche 26 geklebt. Es ist vorteilhaft, wenn die Bauteiloberfläche 26, wie in
Bevor bezugnehmend auf
Wie im vorhergehenden erwähnt ist die Folie 28 dünn ausgestaltet. Im Zusammenhang mit der porösen Membran bietet es zusätzlich zu der Verringerung der Baugröße einen Vorteil, wenn die Folie dünner ausgestaltet ist: aufgrund des an dieser Stelle im Gegensatz zu den Kanalstrukturen verringerten Flussquerschnitts im Bereich zwischen Membran 42 und Oberfläche 26, erhöht sich lokal der Druck, was das Ausgasen über die Membran 42 fördert. So ist der Flussquerschnitt des Durchflusspfades im Bereich der Öffnung 34 kleiner als der durchschnittliche Querschnitt der Kanäle der Kanalstrukturen (d.h. exklusive der Kammern), wie z.B. kleiner als 80% oder sogar kleiner als 50% des letztgenannten. Zudem verhindert eine zu große Dicke der Folie 28 im Bereich der Öffnung 34, dass Gasblasen ohne Berührung der Membran 42 den Bereich der Öffnung 34 passieren können, sodass selbige einem Entweichen über die Membran 42 entgingen, oder anders ausgedrückt fördert eine geringere Dicke die Wahrscheinlichkeit, dass eine Gasblase die Membran berührt, was den Wirkungsgrad der Entgasung fördert.As mentioned above, the
Es soll noch darauf hingewiesen werden, dass, obwohl
Wie es in
In
Bitte kurz zurückkehrend auf die Beschreibung der
Es sollte darauf hingewiesen werden, dass bei den Beispielen/Ausführungsbeispielen von
Nachfolgend wird Bezug nehmend auf die
Wie es in
Gemäß obigen Ausführungsbeispielen kann somit als Folie eine einseitige Klebefolie verwendet werden, wobei die Verwendung einer beidseitig klebenden Folie besonders vorteilhaft sein kann. An der Aussparungsstelle 34 der Folie 28 kann ein Deckel vorgesehen, um einen an dieser Stelle nach oben offenen Kanal zu schließen. Dieser Deckel kann, wie es im Vorhergehenden beschrieben worden ist, ebenfalls in Form einer Folie gestaltet sein. Der Deckel kann sich lateral auf die Aussparungsstelle beschränken. Er verschließt die Aussparungsstelle von oben. Gemäß den oben beschriebenen Ausführungsformen mit einer beidseitigen Klebefolie 28 kann an dieser Stelle der Deckel direkt aufgeklebt werden. Der Deckel muss hier jedoch nicht vollständig geschlossen sein. Wie es im Vorhergehenden beschrieben worden ist, kann der Deckel durch eine poröse Membran gebildet sein. Dies ermöglicht ein Entweichen von ggf. von unerwünschten und ggf. im Kanalsystem vorhandenen Gasblasen. Die poröse Membran kann zudem aus einem Material gebildet sein, welches von der Flüssigkeit nicht benetzt wird. Ist die Flüssigkeit eine wasserbasierte Flüssigkeit, so eignet sich hier besonders eine Membran mit einer Oberfläche oder aus einem Material mit niedriger Oberflächenenergie. Beispiele hierfür sind Fluorpolymere, wie z.B. PTFE, PVDF usw.Thus, according to the above embodiments, a one-sided adhesive film can be used as the film, and the use of a double-sided adhesive film can be particularly advantageous. At the
Insbesondere die leichte Kombinierbarkeit aus konfigurierbaren fluidischen Verbindungen und Blasenfalle stellt einen weiteren Vorteil einiger oben beschriebener Ausführungsbeispiele dar, da hierfür nur drei Teile benötigt werden, nämlich das feststehende Bauteil mit Kanalsystem bzw. Reservoiren, die strukturierte Folie sowie die Deckelmembran.In particular, the easy combinability of configurable fluidic connections and bubble trap represents a further advantage of some embodiments described above, since this only three parts are needed, namely the fixed component with channel system or reservoirs, the structured film and the cover membrane.
Neben dem zuvor beschriebenen selektiven Verbinden von Kanälen kann auf die gezeigte Art und Weise, beispielsweise auch eine Flüssigkeitsquelle (Reservoir), welche nicht benötigt wird, abgetrennt werden. Das ist beispielsweise dann sinnvoll, wenn ein feststehendes mikrofluidisches Teil, wie z.B. eines der Bauteile 22 der oben beschriebenen Ausführungsbeispiele, eine Vielzahl von Reservoiren enthält, für eine bestimmte Anwendung jedoch nur ein Teil hierfür benötigt wird. Würden in einem solchen Fall alle Reservoire mittels Kanäle miteinander verbunden sein, so könnte Flüssigkeit die Luft in diesen Reservoiren komprimieren und so in Richtung dieser leeren (weil nicht benötigten) Reservoire fließen. Bei den oben genannten Ausführungsbeispielen kann das Problem dadurch gelöst werden, dass die Reservoire eben nicht direkt mit dem Kanalsystem verbunden sind, sondern, wie zuvor beschrieben, erst über eine "Brücke" in Form einer Aussparung mit einer Folie miteinander verbunden werden. Für Anwendungen, wo diese Verbindung nicht notwendig ist, wird eben die vor erwähnte Öffnung entsprechend so gestaltet, dass keine Anbindung entsteht.In addition to the selective connection of channels described above can be in the manner shown, for example, a liquid source (reservoir), which is not required to be separated. This is useful, for example, if a fixed microfluidic part, such as e.g. one of the
Bei den oben genannten Ausführungsbeispielen handelt es sich beispielsweise bei den Kammern 32a, 32d und 32e um Reservoire und bei den Kammern 32c und 32b um Sensorstätten, d. h. Orte, an denen Sensoren positioniert sind bzw. positionierbar sind.In the above embodiments, for example, the
Oben genannte Reservoire können auch mit Pumpen versehen sein. Solche Pumpen können mittels Elektrolyse betrieben werden, wie es Vorhergehenden auch beschrieben wurde. Die Elektrolyse erzeugt dabei ein Gas, nämlich in oben erwähnter Kammer 50, und verformt eine Membran, nämlich die verformbare Membran 46, die sich angrenzend zu dem jeweiligen Reservoir befindet. Die Membran kann sich dann in dieses Reservoir verwölben und das darin enthaltene Fluid verdrängen. Sind mehrere dieser Reservoire über ein gemeinsames Kanalsystem verbunden, welches letztlich in einem Auslass oder Abfallbehälter mündet, und enthielte eines der Reservoire anstatt einer Flüssigkeit Luft, so könnte die aus einem Reservoir mittels der Elektrolysepumpe gepumpte Flüssigkeit anstatt in Richtung Auslass/Abfallbehälter in das leere (luftgehüllte) Reservoir fließen. Die einzige Alternative zu obigen "Abtrennen" des Reservoirs mittels geeigneter Platzierung und Ausgestaltung der Folie gemäß obiger Ausführungsbeispiele bestünde lediglich im Füllen auch der nicht benutzten Reservoire, was jedoch einen zusätzlichen Material- und Fertigungsaufwand bedeutete.The above-mentioned reservoirs can also be provided with pumps. Such pumps can be operated by electrolysis, as previously described. The electrolysis thereby generates a gas, namely in the above-mentioned
Die Membran 46, die in diesem Fall, vorzugsweise keine poröse Membran ist, sondern vielmehr vorzugsweise eine sich beispielsweise plastisch verformende Membran, kann sich bei Aufschlagung in einem Druck in Richtung des feststehenden Teils bzw. des Bauteils 22 die Verbindung zwischen zwei Kanälen temporär oder dauerhaft unterbrechen.
Das Beaufschlagen der Membran 46 mit einem Druck in Richtung des Bauteils 22 kann auch als aktives Ventil benutzt werden, wenn beispielsweise unmittelbar oder mittelbar der Druck eines mittels der Elektrolyse erzeugter Gasdruck ist. In diesem Fall muss die Unterbrechung zwischen den Kanälen oder dem Kanal oder dem Reservoir nicht vollständig sein, sondern kann ebenfalls als Vertiefung ausgebildet werden, die jedoch vorzugsweise flacher als der anschließende Kanal ist, wie es bezugnehmend auf
Obige Ausführungsbeispiele beschrieben somit Mikrofluidvorrichtungen, bei denen es möglich war, verschiedene Mikrofluidvorrichtungen auf Basis eines feststehenden mikrofluidischen Teils zu bilden, das für alle identisch ist. Dabei war es möglich, beispielsweise von zwei Sensoren und zwei Flüssigkeitsquellen je eine/n über eine Brücke mit dem Kanalsystem und einer weiteren Flüssigkeitsquelle zu verbinden. Das war beispielsweise bei den Ausgestaltungen nach
Insbesondere beschrieben obige Ausführungsbeispiele somit auch ein mikrofluidisches System, das zumindest ein Teil mit festgelegten Kanalstrukturen aufweist, wobei zumindest zwei Kanalstrukturen im feststehenden Teil zunächst keine Verbindung miteinander aufweisen, die Verbindung stattdessen dadurch hergestellt wird, dass eine Folie, welche die Kanalstrukturen zumindest teilweise bedeckt, eine Öffnung aufweist, die mindestens zwei der nicht verbundenen Kanalstrukturen im feststehenden Teil miteinander verbindet. Zwei im feststehenden Teil nicht miteinander verbundene Kanalstrukturen können zu je einer alternativ bestückbaren Position führen. Ferner kann das mikrofluidische System so gestaltet sein, dass zwei im feststehenden Teil nicht miteinander verbundene Kanalstrukturen von einer anderen Flüssigkeitsquelle bzw. Reservoir kommen. Es ist ebenfalls möglich, dass sich im feststehenden Teil mindestens vier nicht miteinander verbundene Kanalstrukturen befinden, von denen je drei mittels einer Aussparung in einer Folie verbunden werden können, um entweder von zwei alternativen Sensorbereichen oder zwei alternativen Flüssigkeitsquellen eine auszuwählen und mit einer weiteren Flüssigkeitsquelle zu verbinden. Schließlich ist es aber ebenfalls möglich, dass im feststehenden Teil mindestens fünf nicht miteinander verbundene Kanalstrukturen vorhanden sind, von denen drei mittels einer Aussparung in einer Folie verbunden werden können, wobei eine feste Flüssigkeitsquelle mit einen von zwei alternativen Sensorbereichen sowie einer von zwei alternativen Flüssigkeitsquellen verbunden wird. Bei der Folie kann es sich um ein Klebeband handeln, wobei es sich bei dem Klebeband wiederum um ein auf beide Seiten mit einer Klebeschicht versehendes Klebeband handeln kann. Die Aussparung in der Folie ist mit einem Deckel verschlossen. Dieser Deckel weist eine poröse Membran auf. Das Material dieser Membran kann aus einem Material bestehen oder mit selbigem beschichtet sein, das mit dem Kanalsystem zu transportierenden Flüssigkeit einen Kontaktwinkel größer 90° bildet. Die poröse Membran kann aus einem wasserabweisenden Material bestehen, wobei es sich bei dem wasserabweisenden Material auch um ein Fluor enthaltendes Polymer handeln kann. Auf der vom feststehenden Teil abgewandten Seite der Folie kann sich eine Membran befinden, die durch Beaufschlagung mit einem Druck zumindest teilweise in die Aussparung der Folie gedrückt werden kann. Dabei kann der für die Verformung notwendige Druck durch Elektrolyse vom Wasser oder einer zumindest teilweise Wasser enthaltenden Flüssigkeit hervorgerufen werden.In particular, the above embodiments thus also describe a microfluidic system which has at least one part with fixed channel structures, wherein at least two channel structures in the stationary part initially have no connection to one another, the connection is instead produced by a foil which at least partially covers the channel structures, an opening connecting at least two of the unconnected channel structures in the stationary part. Two channel structures not connected to one another in the stationary part can each lead to an alternatively populated position. Furthermore, the microfluidic system may be designed such that two channel structures which are not connected to one another in the stationary part come from a different fluid source or reservoir. It is also possible that there are at least four non-interconnected channel structures in the stationary part, three each of which can be connected by a recess in a foil to select one of two alternative sensor regions or two alternative liquid sources and to another liquid source connect. Finally, it is also possible that at least five non-interconnected channel structures are present in the fixed part, of which three by means of a Recess may be connected in a film, wherein a solid fluid source is connected to one of two alternative sensor areas and one of two alternative fluid sources. The film may be an adhesive tape, wherein the adhesive tape may in turn be an adhesive tape provided with an adhesive layer on both sides. The recess in the film is closed with a lid. This lid has a porous membrane. The material of this membrane may be made of a material or be coated with selbigem that forms a contact angle greater than 90 ° with the channel system liquid to be transported. The porous membrane may be made of a water-repellent material, wherein the water-repellent material may also be a fluorine-containing polymer. On the side facing away from the fixed part of the film, a membrane may be located, which can be at least partially pressed into the recess of the film by applying pressure. In this case, the necessary pressure for the deformation by electrolysis of water or at least partially water-containing liquid can be caused.
Bei der Herstellen einer Vielzahl von unterschiedlichen Mikrofluidvorrichtungen ist es also gemäß obiger Ausführungsbeispiele möglich folgende Schritte zu verwenden: Bereitstellen einer Vielzahl von zumindest einem Bauteil, in dem bzw. in denen Kanalstrukturen gebildet sind, die zumindest teilweise zu einer jeweiligen Bauteiloberfläche des zumindest einen Bauteils offen sind, wobei die Vielzahl untereinander identisch ist; Bereitstellen einer Vielzahl von Folien, die eine Öffnung aufweisen; Fügen einer ersten Teilmenge der Vielzahl von zumindest einem Bauteil und einer ersten Teilmenge der Vielzahl von Folien, so dass über die Öffnung in der jeweiligen Folie zumindest eine erste und eine zweite der Kanalstrukturen miteinander verbunden sind, um erste Mikrofluidvorrichtungen zu bilden; und Fügen einer zweiten Teilmenge der Vielzahl von zumindest einem Bauteil und einer zweiten Teilmenge der Vielzahl von Folien, so dass über die Öffnung in der jeweiligen Folie zumindest eine dritte und eine vierte der Kanalstrukturen miteinander verbunden sind, um zweite Mikrofluidvorrichtungen zu bilden, wobei zumindest beim Fügen der ersten Vielzahl das Fügen so durchgeführt wird, dass die dritte und vierte Kanalstruktur nicht miteinander über die Folie verbunden sind, oder das Fügen der zweiten Vielzahl so durchgeführt wird, dass die erste und zweite Kanalstruktur nicht über die Folie miteinander verbunden werden, so dass sich die ersten und zweiten Mikrofluidvorrichtungen in der Verbindung der Kanalstrukturen unterscheiden.In the production of a plurality of different microfluid devices, it is thus possible according to the above embodiments to use the following steps: providing a plurality of at least one component in which channel structures are formed that are at least partially open to a respective component surface of the at least one component are, wherein the plurality are identical to each other; Providing a plurality of films having an opening; Joining a first subset of the plurality of at least one member and a first subset of the plurality of sheets so that at least a first and a second of the channel structures are interconnected via the opening in the respective sheet to form first microfluidic devices; and joining a second subset of the plurality of at least one component and a second subset of the plurality of films so that at least a third and a fourth of the channel structures are interconnected via the opening in the respective film to form second microfluidic devices, at least at Joining the first plurality, the joining is performed so that the third and fourth channel structure are not connected to each other via the film, or the joining of the second plurality is performed so that the first and second channel structure are not connected to each other via the film, so that the first and second microfluidic devices differ in the connection of the channel structures.
Bezugnehmend auf die vorhergehende Beschreibung wird nun darauf hingewiesen, dass zur Erzielung der unterschiedlichen Kanalstrukturverbindungsmöglichkeiten nicht unbedingt unterschiedliche Folien notwendig sind. Je nach gewünschter Kanalstruktur und Verbindungskombinationen, kann es auch ausreichend sein, zueinander identische Folien auf identische Bauteile aufzukleben, allerdings mit unterschiedlichen Positionen zueinander.Referring now to the foregoing description, it should be understood that different films are not necessarily required to achieve the different channel structure interconnect capabilities. Depending on the desired channel structure and connection combinations, It may also be sufficient to glue identical films to identical components, but with different positions to each other.
Obige Ausführungsbeispiele zeigten also unter anderem eine Mikrofluidvorrichtung mit zumindest einem Bauteil 22, in dem bzw. in denen Kanalstrukturen 24a gebildet sind, die zumindest teilweise zu einer jeweiligen Bauteiloberfläche 26 des zumindest einen Bauteils 22 offen sind; und einer Folie 28, die eine Öffnung 24; 34' aufweist, über die zumindest eine erste und eine zweite der Kanalstrukturen 24a, 24c; 24a, 24b miteinander verbunden sind, und die zumindest eine dritte der Kanalstrukturen an 24b; 24c der jeweiligen Bauteiloberfläche 26 zumindest teilweise verschließt, so dass dieselbe nicht über die Öffnung 34; 34' mit der ersten und zweiten Kanalstruktur verbunden ist. Die erste bis dritte Kanalstruktur können dabei in dem gleichen Bauteil gebildet sein und sich jeweils in der Bauteiloberfläche desselben zumindest teilweise öffnen, wobei die Folie die Bauteiloberfläche des gleichen Bauteils so bedeckt, dass die erste und zweite Kanalstruktur über einen lateral entlang der Bauteiloberfläche des gleichen Bauteils und innerhalb der Öffnung in der Folie führenden Pfad miteinander verbunden sind, während die dritte Kanalstruktur nicht an die Öffnung angrenzt. Der innerhalb der Öffnung 24 in der Folie führende Pfad kann auf einer der Bauteiloberfläche 26 des gleichen Bauteils 22 abgewandten Seite mit einem Deckel verschlossen sein oder mit einem weiteren des zumindest einen Bauteils. Der durch die Öffnung in der Folie führende Pfad auf einer der Bauteiloberfläche des gleichen Bauteils abgewandten Seite mit einer verformbaren Membran als Deckel verschlossen ist Der durch die Öffnung 34 in der Folie 28 führende Pfad ist auf einer der Bauteiloberfläche 26 des gleichen Bauteils 22 abgewandten Seite mit einer porösen Membran 42 als Deckel verschlossen. Insbesondere kann der durch die Öffnung in der Folie führende Pfad auf einer der Bauteiloberfläche des gleichen Bauteils abgewandten Seite mit einer verformbaren Membran als Deckel verschlossen sein. Die Mikrofluidvorrichtung kann ferner einen Aktuator zum Drücken der verformbaren Membran in die Öffnung aufweisen. Die erste bis dritte Kanalstruktur 24a können Vertiefungen in der Bauteiloberfläche des gleichen Bauteils aufweisen, die von der Folie zumindest teilweise abgedeckt sind. Die Vertiefungen können Gräben 30aund/oder Schächte 32a umfassen, um zusammen mit der Folie Kanäle bzw. Kammern zu bilden. Es ist ferner möglich, dass die Folie verformbar ist, und die Mikrofluidvorrichtung ferner einen Aktuator zum Drücken der Folie in eine Vertiefung des zumindest einen Bauteils an der jeweiligen Bauteiloberfläche aufweist, in der die Vertiefung gebildet ist, wobei die Vertiefung Teil der Kanalstrukturen ist. Die Folie 28 kann eine selbstklebende Folie sein. Die Folie 28 kann auch eine beidseitig selbstklebende Folie sein. Ein Aktuator 48 kann ausgebildet sein, um eine zum Drücken notwendige Kraft durch Elektrolyse von Wasser oder einer zumindest teilweise Wasser enthaltenden Flüssigkeit zu erzeugen.Among other things, the above exemplary embodiments have shown a microfluid device with at least one
Claims (12)
- Microfluidic device comprising:at least one component (22) having channel structures (24a) formed therein which are at least partly open toward a respective component surface (26) of the at least one component (22); anda film (28) comprising an elongated opening (24; 34') via which the at least a first and a second one of the channel structures (24a, 24c; 24a, 24b) are connected to one another, the elongated opening being closed by a lid on a side facing away from the component surface (26),wherein the first and second channel structures are formed within the same component and each opens at least partly in the component surface thereof, the film covering the component surface of the same component such that the first and second channel structures are connected to each other via a path extending laterally along the component surface of the same component and within the elongated opening in the film,wherein the channel structures each comprise a trench portion, and the trench portions of the channel structures extend in parallel, over a length portion (60), along a shared extension direction (62) in the component surface of the same component, a longitudinal direction (64) of the elongated opening (34; 34') extending in a manner that is transverse to the shared extension direction (62), and the channel structures projecting into the length portion (60) to different degrees from one and/or two sides, so that a variation of the elongated opening (34) within the film (28) leads, in its position along the extension direction (62) and along the longitudinal direction (64), and in its length along the longitudinal direction (64), to at least four different combinations of channel structure connections,wherein within a combination of channel structure connections, any third channel structures which are formed within the same component as the first and second channel structures and may not be connected to the first and second channel structures via the opening do not adjoin the elongated opening,wherein the channel structures (24a) comprise depressions in the component surface of the same component, said depressions being at least partly covered by the film,wherein the film (28) is adhered to the component surface (26) of the same component (22);characterized in that the path leading through the elongated opening (34) within the film (28) is closed, on a side facing away from the component surface (26) of the same component (22), with a porous membrane (42) as the lid, andwherein the path leading through the opening (34) within the film (28) comprises a flow cross-section which is reduced as compared to the channel structures, so that degassing via the porous membrane (42), which is not wetted by a liquid, is promoted due to a local increase in pressure resulting from the reduced cross-section,wherein a thickness of the film (28) is small enough, in the area of the opening (34), to prevent that gas bubbles in water may pass the path without touching the porous membrane (42).
- Microfluidic device as claimed in claim 1, wherein the at least one component (22) is an injection-molded component.
- Microfluidic device as claimed in claims 1 or 2, wherein the thickness of the film (28) is smaller than 1 mm.
- Microfluidic device as claimed in any of claims 1 to 3, wherein the porous membrane (42) consists of a material, or comprises a surface, which forms a contact angle of more than 90° with water.
- Microfluidic device as claimed in any of claims 1 to 4, wherein the porous membrane consists of or is coated with a fluorine-containing polymer.
- Microfluidic device as claimed in any of claims 1 to 5, wherein the component surface of the same component (22) is planar.
- Microfluidic device as claimed in any of claims 1 to 6, wherein the film comprises a further opening (34') which is sealed by a deformable membrane (46) on a side facing away from the same component (22), the microfluidic device further comprising an actuator for pressing the deformable membrane into the further opening, wherein a flat part of any of the first to third channel structures extends below the further opening, so that due to the deformation, a rate of flow through the flat part may be reduced.
- Microfluidic device as claimed in any of claims 1 to 5, wherein the depressions include trenches (30a) and/or ducts (32a) so as to form channels and/or chambers along with the film.
- Microfluidic device as claimed in any of claims 1 to 8, wherein the first to third channel structures (24a) comprise no mutual fluidic connection within to the first component (22).
- Microfluidic device as claimed in any of the previous claims, wherein the film (28) is a self-adhesive film.
- Method of producing a microfluidic device, comprising;
providing at least one component having channel structures formed therein which are at least partly open toward a respective component surface of the at least one component;
providing a film comprising an opening;
adjoining the at least one component and the film, so that at least a first and a second one of the channel structures are connected to one another via the opening and so that the film at least partly closes at least a third one of the channel structures at the respective component surface, so that same is not connected to the first and second channel structures via the opening; and
closing the elongated opening on a side facing away from the component surface (26) with a lid,
wherein said provision of the at least one component is performed such that the first to third channel structures are formed within the same component and each opens at least partly in a component surface thereof, said joining comprising adhering the film onto the component surface of the same component such that the first and second channel structures are connected to each other via a path extending laterally along the component surface of the same component and within the opening in the film,
wherein the channel structures each comprise a trench portion, and the trench portions of the channel structures extend in parallel, over a length portion (60), along a shared extension direction (62) in the component surface of the same component, a longitudinal direction (64) of the elongated opening (34; 34') extending in a manner that is transverse to the shared extension direction (62), and the channel structures projecting into the length portion (60) to different degrees from one and/or two sides, so that a variation of the elongated opening (34) within the film (28) leads, in its position along the extension direction (62) and along the longitudinal direction (64), and in its length along the longitudinal direction (64), to at least four different combinations of channel structure connections,
wherein within a combination of channel structure connections, any third channel structures which are formed within the same component as the first and second channel structures and may not be connected to the first and second channel structures via the opening do not adjoin the elongated opening,
wherein the channel structures (24a) comprise depressions in the component surface of the same component, said depressions being at least partly covered by the film,
characterized in that the path leading through the elongated opening (34) within the film (28) is closed, on a side facing away from the component surface (26) of the same component (22), with a porous membrane (42) as the lid, and
wherein the path leading through the opening (34) within the film (28) comprises a flow cross-section which is reduced as compared to the channel structures, so that degassing via the porous membrane (42), which is not wetted by a liquid, is promoted due to a local increase in pressure resulting from the reduced cross-section,
wherein a thickness of the film (28) is small enough, in the area of the opening (34), to prevent that gas bubbles in water may pass the path without touching the porous membrane (42). - Method as claimed in claim 11, wherein the film is a self-adhesive film and wherein adhesion comprises merely applying the film onto the component surface of the same component.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102010061910.8A DE102010061910B4 (en) | 2010-11-24 | 2010-11-24 | Microfluidic device and method of making same |
EP11787682A EP2521619A2 (en) | 2010-11-24 | 2011-11-23 | Microfluidic device and method for producing the same |
Related Parent Applications (2)
Application Number | Title | Priority Date | Filing Date |
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EP11787682A Division EP2521619A2 (en) | 2010-11-24 | 2011-11-23 | Microfluidic device and method for producing the same |
EP11787682.1 Division | 2011-11-23 |
Publications (2)
Publication Number | Publication Date |
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EP2522427A1 EP2522427A1 (en) | 2012-11-14 |
EP2522427B1 true EP2522427B1 (en) | 2018-06-20 |
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Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
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EP12179918.3A Active EP2522427B1 (en) | 2010-11-24 | 2011-11-23 | Micro-fluid device and method for manufacturing the same |
EP11787682A Withdrawn EP2521619A2 (en) | 2010-11-24 | 2011-11-23 | Microfluidic device and method for producing the same |
Family Applications After (1)
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EP11787682A Withdrawn EP2521619A2 (en) | 2010-11-24 | 2011-11-23 | Microfluidic device and method for producing the same |
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EP (2) | EP2522427B1 (en) |
DE (1) | DE102010061910B4 (en) |
WO (1) | WO2012069527A2 (en) |
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DE102013100075A1 (en) | 2013-01-07 | 2014-07-10 | Technische Universität Chemnitz | Micro-fluidic system, has system components thermally connected with each other, where membrane is located in recess that is formed in system components, which comprise structure raising from recess and clamping membrane |
SG11201700087UA (en) | 2014-08-05 | 2017-02-27 | Sanwa Biotech Ltd | On-site diagnostic system and the method thereof |
DE102020135053B4 (en) | 2020-12-29 | 2022-12-15 | Biflow Systems Gmbh | Microfluidic device with residue container and analysis system |
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US20020187074A1 (en) * | 2001-06-07 | 2002-12-12 | Nanostream, Inc. | Microfluidic analytical devices and methods |
US20050266582A1 (en) * | 2002-12-16 | 2005-12-01 | Modlin Douglas N | Microfluidic system with integrated permeable membrane |
US20080262213A1 (en) * | 2004-05-03 | 2008-10-23 | Betty Wu | Processing Polynucleotide-Containing Samples |
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US20020112961A1 (en) * | 1999-12-02 | 2002-08-22 | Nanostream, Inc. | Multi-layer microfluidic device fabrication |
US6686184B1 (en) * | 2000-05-25 | 2004-02-03 | President And Fellows Of Harvard College | Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks |
DE10055374B4 (en) * | 2000-11-08 | 2006-03-02 | Bartels Mikrotechnik Gmbh | Distributor plate for liquids and gases |
WO2002083310A2 (en) * | 2001-04-13 | 2002-10-24 | Nanostream, Inc. | Microfluidic metering systems and methods |
US7318912B2 (en) * | 2001-06-07 | 2008-01-15 | Nanostream, Inc. | Microfluidic systems and methods for combining discrete fluid volumes |
US20020187072A1 (en) * | 2001-06-07 | 2002-12-12 | Nanostream, Inc. | Multi-layer microfluidic splitter |
US6958119B2 (en) * | 2002-02-26 | 2005-10-25 | Agilent Technologies, Inc. | Mobile phase gradient generation microfluidic device |
DE102009001257A1 (en) * | 2008-10-06 | 2010-04-15 | Aj Ebiochip Gmbh | Apparatus and method for handling liquids |
-
2010
- 2010-11-24 DE DE102010061910.8A patent/DE102010061910B4/en active Active
-
2011
- 2011-11-23 EP EP12179918.3A patent/EP2522427B1/en active Active
- 2011-11-23 EP EP11787682A patent/EP2521619A2/en not_active Withdrawn
- 2011-11-23 WO PCT/EP2011/070788 patent/WO2012069527A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020187074A1 (en) * | 2001-06-07 | 2002-12-12 | Nanostream, Inc. | Microfluidic analytical devices and methods |
US20050266582A1 (en) * | 2002-12-16 | 2005-12-01 | Modlin Douglas N | Microfluidic system with integrated permeable membrane |
US20080262213A1 (en) * | 2004-05-03 | 2008-10-23 | Betty Wu | Processing Polynucleotide-Containing Samples |
Also Published As
Publication number | Publication date |
---|---|
EP2521619A2 (en) | 2012-11-14 |
DE102010061910B4 (en) | 2016-04-28 |
EP2522427A1 (en) | 2012-11-14 |
DE102010061910A1 (en) | 2012-05-24 |
WO2012069527A3 (en) | 2012-08-16 |
WO2012069527A2 (en) | 2012-05-31 |
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