EP2449125A4 - Kombinierte nukleinsäureblockierung, -extraktion und -erkennung in einem einzelreaktionsgefäss - Google Patents

Kombinierte nukleinsäureblockierung, -extraktion und -erkennung in einem einzelreaktionsgefäss

Info

Publication number
EP2449125A4
EP2449125A4 EP10794424.1A EP10794424A EP2449125A4 EP 2449125 A4 EP2449125 A4 EP 2449125A4 EP 10794424 A EP10794424 A EP 10794424A EP 2449125 A4 EP2449125 A4 EP 2449125A4
Authority
EP
European Patent Office
Prior art keywords
extraction
detection
nucleic acid
reaction vessel
single reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10794424.1A
Other languages
English (en)
French (fr)
Other versions
EP2449125A2 (de
Inventor
David James Saul
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ZyGEM Corp Ltd
Original Assignee
ZyGEM Corp Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ZyGEM Corp Ltd filed Critical ZyGEM Corp Ltd
Publication of EP2449125A2 publication Critical patent/EP2449125A2/de
Publication of EP2449125A4 publication Critical patent/EP2449125A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP10794424.1A 2009-07-02 2010-07-02 Kombinierte nukleinsäureblockierung, -extraktion und -erkennung in einem einzelreaktionsgefäss Withdrawn EP2449125A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22291209P 2009-07-02 2009-07-02
PCT/NZ2010/000137 WO2011002319A2 (en) 2009-07-02 2010-07-02 Combined nucleic acid blocking, extraction, and detection in a single reaction vessel

Publications (2)

Publication Number Publication Date
EP2449125A2 EP2449125A2 (de) 2012-05-09
EP2449125A4 true EP2449125A4 (de) 2013-05-29

Family

ID=43411643

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10794424.1A Withdrawn EP2449125A4 (de) 2009-07-02 2010-07-02 Kombinierte nukleinsäureblockierung, -extraktion und -erkennung in einem einzelreaktionsgefäss

Country Status (3)

Country Link
EP (1) EP2449125A4 (de)
JP (1) JP2012531907A (de)
WO (1) WO2011002319A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100041056A1 (en) * 2008-08-14 2010-02-18 Zygem Corporation Limited Temperature controlled nucleic-acid detection method suitable for practice in a closed-system
PL2478505T3 (pl) * 2009-09-16 2017-09-29 John J. Fischer Standardowe protokoły bezpieczeństwa i zabezpieczenia przed rozproszeniem uwagi przez mobilne urządzenie komunikacyjne
US9294603B2 (en) 2009-09-16 2016-03-22 Try Safety First, Inc. Standard mobile communication device distraction prevention and safety protocols
WO2014006620A1 (en) 2012-07-05 2014-01-09 P.C.O.A. Devices Ltd. Medication dispenser
WO2014025500A2 (en) * 2012-07-16 2014-02-13 Microlab Horizon Llc Thermostable enzyme-based extractions on an integrated microfluidic chip for biological analysis
WO2014020594A2 (en) 2012-07-30 2014-02-06 P.C.O.A. Devices Ltd. A receptacle for containing and dispensing solid medicinal pills
IL233295B (en) 2014-06-22 2019-11-28 Ilan Paz A control pill dispensing system
WO2016065300A1 (en) * 2014-10-24 2016-04-28 Eshoo Mark W Microfluidic cartridge
JP6481364B2 (ja) * 2014-12-26 2019-03-13 東洋紡株式会社 核酸除去法
IL238387B (en) 2015-04-20 2019-01-31 Paz Ilan Drug dispenser release mechanism
EP3362030B1 (de) 2015-10-15 2023-09-06 Dosentrx Ltd. Auf bilderkennung basierende darreichungsformspender
US11458072B2 (en) 2015-11-02 2022-10-04 Dosentrx Ltd. Lockable advanceable oral dosage form dispenser containers
CN107988046B (zh) * 2018-01-23 2021-02-19 吉林大学 基于lamp的自吸式多通道病原菌检测微流控芯片
JP2020162586A (ja) * 2019-03-29 2020-10-08 国立研究開発法人農業・食品産業技術総合研究機構 生物の死亡時期の判定方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010019898A1 (en) * 2008-08-14 2010-02-18 Zygem Corporation Limited Temperature controlled nucleic-acid detection method suitable for practice in a closed-system

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0572057A1 (de) * 1992-05-11 1993-12-01 Johnson & Johnson Clinical Diagnostics, Inc. PCR-Reagenzien- und Testsatz und Verfahren für die Amplifikation und Detektion mit verringerter, nicht-spezifischer Amplifikation von Nukleinsäuren
US20080220425A1 (en) * 1996-01-24 2008-09-11 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
NZ511680A (en) * 2001-05-14 2004-07-30 Univ Waikato Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
EP2159285B1 (de) * 2003-01-29 2012-09-26 454 Life Sciences Corporation Verfahren zur Verstärkung und Sequenzierung von Nukleinsäuren
ATE532875T1 (de) * 2004-06-17 2011-11-15 Zygem Corp Ltd Reinigungsverfahren und ihre verwendungen
GB0414815D0 (en) * 2004-07-02 2004-08-04 Secr Defence Method for stabilising reagents which are useful for nucleic acid amplification
US20080026429A1 (en) * 2006-03-13 2008-01-31 Waikatolink Limited Solution pre-treatment
NZ548731A (en) * 2006-07-24 2008-12-24 Zygem Corp Ltd Isothermal detection methods and uses thereof
JP2008048648A (ja) * 2006-08-23 2008-03-06 Institute Of Physical & Chemical Research 核酸増幅用プライマーセット及び核酸の増幅方法
JP5547071B2 (ja) * 2007-08-09 2014-07-09 セルラ・インコーポレイテッド 関連付け多パラメーター単一細胞測定および残留する生物学的材料の回収のための方法および装置

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010019898A1 (en) * 2008-08-14 2010-02-18 Zygem Corporation Limited Temperature controlled nucleic-acid detection method suitable for practice in a closed-system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MOSS D ET AL: "An easily automated, closed-tube forensic DNA extraction procedure using a thermostable proteinase", INTERNATIONAL JOURNAL OF LEGAL MEDICINE, SPRINGER VERLAG, DE, vol. 117, no. 6, 1 December 2003 (2003-12-01), pages 340 - 349, XP002476705, ISSN: 0937-9827, [retrieved on 20031023], DOI: 10.1007/S00414-003-0400-9 *

Also Published As

Publication number Publication date
EP2449125A2 (de) 2012-05-09
WO2011002319A2 (en) 2011-01-06
JP2012531907A (ja) 2012-12-13
WO2011002319A3 (en) 2011-04-14

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