EP2398495B2 - Modified non-cytotoxic proteases - Google Patents

Modified non-cytotoxic proteases Download PDF

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EP2398495B2
EP2398495B2 EP09799683.9A EP09799683A EP2398495B2 EP 2398495 B2 EP2398495 B2 EP 2398495B2 EP 09799683 A EP09799683 A EP 09799683A EP 2398495 B2 EP2398495 B2 EP 2398495B2
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protein
protease
site
polypeptide
amino acid
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EP2398495B1 (en
EP2398495B8 (en
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John Andrew Chaddock
Keith Alan Foster
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Ipsen Bioinnovation Ltd
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    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
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    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Definitions

  • the present invention relates to non-cytotoxic proteases having improved efficacy, and to the construction thereof.
  • Non-cytotoxic proteases are a well-recognised group of proteases, which act on target cells by incapacitating cellular function. Importantly, non-cytotoxic proteases do not kill the target cells upon which they act. Some of the best known examples of non-cytotoxic proteases include clostridial neurotoxins (e.g. botulinum neurotoxin; also known as BOTOXTM) and IgA proteases.
  • Non-cytotoxic proteases act by proteolytically-cleaving intracellular transport proteins known as SNARE proteins (e.g. SNAP-25, VAMP, or Syntaxin) - see Gerald K (2002) "Cell and Molecular Biology” (4th edition) John Wiley & Sons, Inc.
  • the acronym SNARE derives from the term S oluble N SF A ttachment R eceptor, where NSF means N -ethylmale-imide- S ensitive Factor.
  • SNARE proteins are integral to intracellular vesicle formation, and thus to secretion of molecules via vesicle transport from a cell. Accordingly, once delivered to a desired target cell, the non-cytotoxic protease is capable of inhibiting cellular secretion from the target cell.
  • Non-cytotoxic proteases may be employed in their native or substantially native forms (i.e. as holotoxins, such as BOTOXTM), in which case targeting of the proteases to specific cell-types is reliant on (i) localised administration of the protease and/ or (ii) the inherent binding ability of the native protease.
  • non-cytotoxic proteases may be employed in a re-targeted form in which the native protease is modified to include an exogenous ligand known as a Targeting Moiety (TM).
  • the TM is selected to provide binding specificity for a desired target cell, and, as part of the re-targeting process, the native binding portion of the non-cytotoxic protease may be removed.
  • Re-targeting technology is described, for example, in: EP-B-0689459 ; EP-B-0939818 ; US 6,461,617 ; US 7,192,596 ; EP-B-0826051 ; US 5,989,545 ; US 6,395,513 ; US 6,962,703 ; EP-B-0996468 ; US 7,052,702 ; EP-B-1107794 ; and US 6,632,440 .
  • non-cytotoxic proteases have been successfully employed in a wide range of therapies.
  • BOTOXTM botulinum neurotoxins
  • BoNTs botulinum neurotoxins
  • BoNT/A botulinum neurotoxins
  • BoNT/B BoNT/C1
  • BoNT/D BoNT/E
  • BoNT/F BoNT/F
  • BoNT/G tetanus neurotoxin
  • TeNT tetanus neurotoxin
  • BOTOXTM is currently approved as a therapeutic for the following indications: achalasia, adult spasticity, anal fissure, back pain, blepharospasm, bruxism, cervical dystonia, essential tremor, glabellar lines or hyperkinetic facial lines, headache, hemifacial spasm, hyperactivity of bladder, hyperhidrosis, juvenile cerebral palsy, multiple sclerosis, myoclonic disorders, nasal labial lines, spasmodic
  • clostridial toxin therapies are described for treating neuromuscular disorders (see US 6,872,397 ; for treating uterine disorders (see US2004/0175399 ); for treating ulcers and gastroesophageal reflux disease (see US2004/0086531 ); for treating dystonia (see US 6,319,505 ); for treating eye disorders (see US2004/0234532 ); for treating blepharospasm (see US2004/0151740 ); for treating strabismus (see US2004/0126396 ); for treating pain (see US 6,869,610 , US 6,641,820 , US 6,464,986 , US 6,113,915 ); for treating fibromyalgia (see US 6,623,742 , US2004/0062776 ); for treating lower back pain (see US2004/0037852 ); for treating muscle injuries (see US 6,423,319 ); for treating sinus headache (see US 6,838,434 ); for treating tension headache (see US 6,
  • non-cytotoxic proteases such as clostridial neurotoxins (e.g. BoNTs and TeNT) in therapeutic and cosmetic treatments of humans and other mammals is anticipated to expand to an ever-widening range of diseases and ailments that can benefit from the properties of these toxins.
  • Non-cytotoxic protease including native clostridial neurotoxin clinical products
  • Larger doses can increase the likelihood that the protease may move, for example, through the interstitial fluids and the circulatory systems (such as the cardiovascular system and the lymphatic system) of the body, resulting in undesirable dispersal of the protease to areas not targeted for treatment. Said dispersal can lead to undesirable side effects, such as inhibition of cellular secretion in cells not targeted for treatment (e.g. inhibition of neurotransmitter release in neurons not targeted for treatment, or paralysis of a muscle not targeted for treatment).
  • a patient administered a therapeutically effective amount of a BoNT into the neck muscles for torticollis may develop dysphagia because of dispersal of the protease into the oropharynx.
  • a patient administered a non-cytotoxic protease to treat a neuromuscular disorder may suffer from undesirable muscle tissue inactivation due to dispersal of the protease into the muscle.
  • a therapeutic dosing range exists which identifies the lower and upper limits of safe, effective therapy.
  • the upper limit is determined by the increasing significance of off-target effects that lead to undesirable (e.g. potentially harmful) side-effects of drug treatment.
  • non-cytotoxic proteases notably BoNT
  • Lin, Wei-Jen, et al. seek to solve this problem by provision of clostridial neurotoxins modified to contain a blood protease cleavage site (ie. a site cleavable by a protease present in blood) in the binding domain of the neurotoxin, such that contact with a blood protease selectively inactivates the neurotoxin.
  • Said binding domain (also referred to as the H C domain) is illustrated in Figure 1B of Lin, Wei-Jen, et al. as the region starting at amino acid residue 873.
  • non-cytotoxic proteases may be employed in a re-targeted form in which the native protease is modified to include an exogenous ligand known as a Targeting Moiety (TM), which provides binding specificity for a desired target cell.
  • TM Targeting Moiety
  • the present invention addresses the deficiencies of Lin, et al . and provides non-cytotoxic proteases that reduce or prevent unwanted side-effects associated with dispersal into non-targeted areas. These and related advantages are useful for various clinical, therapeutic and cosmetic applications, such as the treatment of neuromuscular disorders, neuropathic disorders, eye disorders, pain, muscle injuries, headache, cardiovascular diseases, neuropsychiatric disorders, endocrine disorders, exocrine disorders, mucus secretion-related disorders such as asthma and COPD, cancers, otic disorders and hyperkinetic facial lines, as well as, other disorders where non-cytotoxic protease administration to a mammal can produce a beneficial effect (e.g. all of the therapies described on pages 2-3 of this specification).
  • the present invention provides polypeptides as specified in the claims. Also provided are nucleic acids encoding the polypeptides of the invention.
  • the present invention provides a polypeptide that can be controllably inactivated and/ or destroyed at an off-site location.
  • the destructive cleavage site is recognised and cleaved by a second protease (i.e. a destructive protease) selected from a circulating protease (e.g. an extracellular protease, such as a serum protease or a protease of the blood clotting cascade), a tissue-associated protease (e.g. a matrix metalloprotease (MMP), such as a MMP of muscle), and an intracellular protease (preferably a protease that is absent from the target cell)).
  • a circulating protease e.g. an extracellular protease, such as a serum protease or a protease of the blood clotting cascade
  • a tissue-associated protease e.g. a matrix metalloprotease (MMP), such as a MMP of muscle
  • MMP matrix metalloprotease
  • intracellular protease preferably a protease that is absent from
  • polypeptide of the present invention when a polypeptide of the present invention become dispersed away from its intended target cell and/ or be taken up by a non-target cell, the polypeptide will become inactivated by cleavage of the destructive cleavage site (by the second protease).
  • the destructive cleavage site is recognised and cleaved by a second protease that is present within an off-site cell-type.
  • the off-site cell and the target cell are preferably different cell types.
  • the destructive cleavage site is recognised and cleaved by a second protease that is present at an off-site location (e.g. distal to the target cell).
  • the target cell and the off-site cell may be either the same or different cell-types.
  • the target cell and the off-site cell may each possess a receptor to which the same polypeptide of the invention binds).
  • a polypeptide of the present invention when treating neuromuscular disorders, is targeted to the desired target cell (e.g. to a motor neuron), and includes a destructive protease cleavage site that is cleaved by a second protease present within and/ or in close proximity to muscle tissue. Accordingly, the polypeptide demonstrates minimal adverse effects on muscle tissue, and can be used at greater doses than currently tolerable by a patient, thereby leading to enhanced clinical efficacy.
  • the destructive cleavage site of the present invention provides for inactivation/ destruction of the polypeptide when the polypeptide is in or at an off-site location.
  • cleavage at the destructive cleavage site minimises the potency of the polypeptide by reducing the inherent ability of the polypeptide (when compared with an identical polypeptide lacking the same destructive cleavage site, or possessing the same destructive site but in an uncleaved form) to translocate the non-cytotoxic component (across the endosomal membrane of a mammalian cell in the direction of the cytosol), and/ or to effect SNARE protein cleavage.
  • the polypeptide of the invention may include a second (or subsequent) inactivation/ destruction site.
  • Said (or subsequent) second site may be located anywhere within the polypeptide (including within the TM component).
  • Said second (or subsequent) site may be cleaved by the same or by a different protease.
  • Said second (or subsequent) site may have a different amino acid recognition sequence that the first inactivation/ destruction site, and may be cleaved by the same or by a different protease.
  • the above-mentioned reduced SNARE cleavage and/ or reduced translocation capacity can be readily measured by direct comparison of a polypeptide of the invention with an identical polypeptide (though lacking the same destructive cleavage site, or possessing the same destructive site but in an uncleaved form).
  • the polypeptide of the invention and the corresponding uncleaved counterpart may be assayed in parallel in any one of a variety of conventional whole cell or cell free assays.
  • Examples 1-4 By way of example, reference is made to Examples 1-4.
  • the polypeptide of the invention becomes inactivated (via cleavage at the destructive cleavage site), whereas the counterpart polypeptide substantially retains full potency.
  • a polypeptide of the invention when cleaved at the destructive cleavage site, possesses less than 50% or less than 25%, less than 10% or less than 5%, less than 1% or less than 0.5%, less than 0.1 % or less than 0.01%, or less than 0.001% or less than 0.0001% of the SNARE protein cleavage ability and/ or reduced translocation ability when compared with the uncleaved counterpart polypeptide.
  • reduced SNARE cleavage and/ or reduced translocation ability may be determined by measuring relative SNARE protein cleavage in a mammalian cell. This is reflective of the overall ability of the polypeptide to translocate into and subsequently cleave a SNARE protein within the cytosol of a mammalian cell.
  • SNARE protein cleavage such as, for example, SDS-PAGE and Western Blotting followed by densitometer analysis of the cleaved products.
  • potency can be measured in terms of relative SNARE protein cleavage, or in terms of relative translocation function (e.g. release of K + or NAD from liposomes, or membrane conductance measurements).
  • Preferred off-site targets include: epithelial cells, especially lung epithelial cells; neuronal cells that are not motor neuron cells; and muscle cells.
  • a modified clostridial neurotoxin (LH N /C) was provided.
  • This neurotoxin mimics the modified neurotoxin of Lin, et al. (ie. as discussed in the background part of this specification) as it lacks a functional H C binding domain.
  • Said modified neurotoxin was incubated in the presence of a mammalian cell (e.g. a non-human embryonic spinal cord neuron (eSCN)) to assess it's ability to demonstrate residual clostridial neurotoxin activity in the form of SNARE protein cleavage.
  • a mammalian cell e.g. a non-human embryonic spinal cord neuron (eSCN)
  • a control neurotoxin consisting solely of the endopeptidase domain of botulinum neurotoxin type C (LC/C) was incubated in the same manner - the control neurotoxin therefore lacked a function H N translocation domain.
  • Each of the two polypeptides was then assessed for cleavage of a SNARE protein in the test cell.
  • the LH N /C modified clostridial neurotoxin demonstrated SNARE cleavage (see Figure 1 ), and thus confirmed that inactivation of the H C binding domain of botulinum neurotoxin is not adequate to reduce off-site activity.
  • the control neurotoxin (lacking a functional translocation component) demonstrated a lack of SNARE cleavage.
  • the polypeptide of the present invention may include one or more (e.g. two, three, four, five or more) destructive protease cleavage sites. Where more than one destructive cleavage site is included, each cleavage site may be the same or different. In this regard, use of more than one destructive cleavage sites provides improved off-site inactivation. Similarly, use of two or more different destructive cleavage sites provides additional design flexibility. For example, when minimising off-site target effects in muscle tissue, the polypeptide of the present invention may include two different destructive sites, which are recognised and cleaved by two different muscle tissues associated proteases.
  • the first destructive cleavage site(s) may be engineered into the non-cytotoxic protease component or the translocation component.
  • the second (or subsequent) site(s) may be engineered anywhere into the polypeptide.
  • the destructive cleavage site(s) are chosen to ensure minimal adverse effect on the potency of the polypeptide (for example by having minimal effect on the translocation domain, and/ or on the non-cytotoxic protease domain) whilst ensuring that the polypeptide is labile away from its target site/ target cell.
  • Preferred destructive cleavage sites are listed in the Table immediately below. The listed cleavage sites are purely illustrative and are not intended to be limiting to the present invention.
  • Second protease Destructive cleavage site recognition sequence Tolerated recognition sequence variance P4-P3-P2-P1- ⁇ -P1'-P2'-P3' P4 P3 P2 P1 P1' P2' P3' Thrombin LVPR ⁇ GS A,F,G,I,L ,T,V or M A,F,G,I, L,T,V,W or A P R Not D or E Not D or E --- Thrombin GR ⁇ G G R G Factor Xa IEGR ⁇ A,F,G,I,L ,T,V or M DorE G R --- --- --- ADAM17 PLAQA ⁇ VRSSS Human airway trypsin-like protease (HAT) SKGR ⁇ SLIGRV ACE (peptidy
  • the present invention may employ destructive cleavage sites that are cleavable by a mammalian blood protease, such as Thrombin, Coagulation Factor VIIa, Coagulation Factor IXa, Coagulation Factor Xa, Coagulation Factor XIa, Coagulation Factor XIIa, Kallikrein, Protein C, and MBP-associated serine protease.
  • a mammalian blood protease such as Thrombin, Coagulation Factor VIIa, Coagulation Factor IXa, Coagulation Factor Xa, Coagulation Factor XIa, Coagulation Factor XIIa, Kallikrein, Protein C, and MBP-associated serine protease.
  • Lin, et al. describe the use of thrombin or Factor Xa cleavage sites to inactivate the H C binding domain of a clostridial holotoxin. As discussed above, however, H C inactivation is inadequate to achieve desirable off-site inactivation, Moreover, due to the pausity of cleavage sites disclosed, the method described by Lin, et al. has limited utility, for example in off-site environments where thrombin and Factor Xa are absent (or only present at low concentrations).
  • Matrix metalloproteases are a preferred group of destructive proteases in the context of the present invention.
  • ADAM17 EC 3.4.24.86
  • TACE TACE
  • MMPs matrix metalloproteases
  • Additional, preferred MMPs include adamalysins, serralysins, and astacins.
  • said destructive cleavage site(s) comprises a recognition sequence having at least 3 or 4, preferably 5 or 6, more preferably 6 or 7, and particularly preferably at least 8 contiguous amino acid residues.
  • the longer (in terms of contiguous amino acid residues) the recognition sequence the less likely non-specific cleavage of the destructive site will occur via an unintended second protease.
  • the polypeptide of the present invention includes a Targeting Moiety (TM) that binds to a Binding Site on a neuronal (eg. nerve) cell, thereby providing selectivity of the polypeptide to this species of target cell over other cells.
  • TM Targeting Moiety
  • the neuronal cell is a cell of the neuromuscular junction or presynaptic cholinergic peripheral nerve terminal.
  • the first (and subsequent) destructive cleavage site(s) of the present invention is preferably introduced into the protease component and/ or into the translocation component.
  • the protease component is preferred.
  • the polypeptide may be rapidly inactivated by direct destruction of the non-cytotoxic protease and/ or translocation components.
  • These insertion positions are preferable over a TM insertion position because, even in the case of total TM inactivation, it has been shown that the resulting polypeptide may not demonstrate adequately reduced potency on off-site cells [ Chaddock, JA., et al. Protein Expression Purification 2002, 25, 219-228 and Sutton, JM, et al. Protein Expression & Purification 2005, 40(1), 31-41 ].
  • the polypeptide of the present invention does not comprise a destructive cleavage site(s) solely within the Targeting Moiety component of the polypeptide.
  • a destruction site within the TM component alone does not address non-specific uptake by off-site target cells.
  • Example 39 (see also Figure 1 ) demonstrates that a fragment of botulinum neurotoxin type C lacking the binding domain H C is still able to enter eSCN and cleave its substrate SNARE protein (syntaxin).
  • cleavage within the TM component might lead to a TM having increased binding affinity for off-site cells, for example, via exposure of a higher affinity binding region within the TM.
  • the use of a destructive cleavage site(s) within the TM component alone is considered unsatisfactory.
  • off-site targeting is not adequately addressed, and, secondly, once delivered to an off-site cell, the polypeptides are still capable of (wild-type/ natural) translocation activity and/ or SNARE protein cleavage activity.
  • the TM has no destructive cleavage site.
  • the TM component may be particularly susceptible to adverse conformational changes (upon insertion of a destructive cleavage site), which adversely affect desired binding of the polypeptide. This has been shown to be a particular problem when the TM is the native targeting moiety of a clostridial neurotoxin (i.e. H C ).
  • Suitable TMs for use in the polypeptides of the present invention include cytokines, growth factors, neuropeptides, lectins, protein binding scaffolds, and antibodies - this term includes monoclonal antibodies, and antibody fragments such as Fab, F(ab)' 2 , Fv, ScFv, etc.
  • the TM is a ligand (preferably a peptide) that binds to a neuronal cell, preferably to a neuronal cell of the neuromuscular junction.
  • the TM comprises the binding domain (H CC , or H C ) of a clostridial neurotoxin (e.g. BoNT, TeNT, or from other Clostridium spp .), or a fragment thereof that possesses native neurotoxin binding ability.
  • the clostridial H C domain has evolved to bind in a highly effective manner to receptors present on neuronal cells.
  • the present invention provides use and corresponding methods for modifying BOTOXTM to improve its clinical utility.
  • suitable TM clostridial H CC reference sequences include:
  • suitable TM clostridial H C domains of reference sequences include: BoNT/A - N872-L1296; BoNT/B - E859-E1291; BoNT/C1-N867-E1291; BoNT/D - S863-E1276; BoNT/E - R846-K1252; BoNT/F - K865-E1274; BoNT/G - N864-E1297; and TeNT - I880-D1315.
  • the TM is selected to provide desirable binding to the neuromuscular junction.
  • Suitable TMs are listed in WO 2006/099590 , and include: glucagon like hormone, a neurohormone, a neuroregulatory cytokine, a neurotrophin, a growth factor, an axon guidance signaling molecule, a sugar binding protein, a ligand that selectively binds a neurexin, a ligand for neurexin-2 ⁇ , a ligand for neurexin-2 ⁇ , a ligand for neurexin-3 ⁇ , a ligand for neurexin-3 ⁇ , a WNT, Ng-CAM(LI), NCAM, N-cadherin, a PACAP peptide such as a VIP peptide, Agrin-MUSK, a basement membrane polypeptide, and a variant of any of the foregoing polypeptides, a neuroregulatory cytokine such as ciliary neurotrophic factor (CNTF), glycophor
  • the destructive cleavage site(s) are introduced with minimum adverse effect on the biological properties of the polypeptide (notably, endopeptidase activity, and/ or membrane translocation activity).
  • any potential decrease in potency of the polypeptide is less than 25%, preferably less than 15%, more preferably less than 5% of the original unmodified protein. Potency here may be measured by a comparative assay such as illustrated in Examples 1-4.
  • the destructive cleavage site(s) are not substrates for any proteases that may be separately used for post-translational modification of the polypeptide of the present invention as part of its manufacturing process.
  • the non-cytotoxic proteases of the present invention typically employ a protease activation event (via a separate 'activation' protease cleavage site, which is structurally distinct from the destructive cleavage site of the present invention).
  • the purpose of the activation cleavage site is to cleave a peptide bond between the non-cytotoxic protease and translocation or TM components of the polypeptide of the present invention, thereby providing an 'activated' di-chain polypeptide wherein said two components are linked together via a di-sulfide bond.
  • the di-chain loop protease cleavage site occurs at K448-A449 for BoNT/A, at K441-A442 for BoNT/B, at K449-T450 for BoNT/C1, at R445-D446 for BoNT/D, at R422-K423 for BoNT/E, at K439-A440 for BoNT/F, at K446-S447 for BoNT/G, and at A457-S458 for TeNT.
  • the former is preferably introduced into polypeptide of the present invention at a position of at least 20, at least 30, at least 40, at least 50, and more preferably at least 60, at least 70, at least 80 (contiguous) amino acid residues away from the 'activation' cleavage site.
  • the activation site of a polypeptide of the invention may be readily aligned (via simple, primary sequence alignment) with the activation site positions (listed above) for clostridial holotoxin.
  • the first destructive cleavage site is exogenous (i.e. engineered/ artificial) with regard to the native components of the polypeptide.
  • said cleavages site is not inherent to the corresponding native components of the polypeptide.
  • a protease or translocation component based on BoNT/A L-chain or H-chain may be engineered according to the present invention to include a cleavage site(s). Said cleavage site(S) would not, however, be present in the corresponding BoNT native L-chain or H-chain.
  • the destructive cleavage site(s) and the 'activation' cleavage site are not cleaved by the same protease.
  • the two cleavage sites differ from one another in that at least one, more preferably at least two, particularly preferably at least three, and most preferably at least four of the tolerated amino acids within the respective recognition sequences is/ are different.
  • a destructive cleavage site(s) that is a site other than a Factor Xa site, which may be inserted elsewhere in the L-chain and/ or H N component(s).
  • the polypeptide may be modified to accommodate an alternative 'activation' site between the L-chain and H N components (for example, an enterokinase cleavage site), in which case a separate Factor Xa cleavage site(s) may be incorporated elsewhere into the polypeptide as the destructive cleavage site.
  • the existing Factor Xa 'activation' site between the L-chain and H N components may be retained, and an alternative cleavage site such as a thrombin cleavage site incorporated as the destructive cleavage site(s).
  • cleavage sites typically comprise at least 3 contiguous amino acid residues.
  • a cleavage site is selected that already possesses (in the correct position(s)) at least one, preferably at least two of the amino acid residues that are required in order to introduce the new cleavage site.
  • a preferred insertion position may be identified that already includes a primary sequence selected from, for example, Dxxx, xMxx, xxQx, xxxD, DMxx, DxQx, DxxD, xMQx, xMxD, xxQD, DMQx, xMQD, DxQD, and DMxD.
  • BoNT Serotype PDB ID PDB Description A 1E1H Crystal structure of recombinant botulinum neurotoxin type A light chain, self-inhibiting Zn endopeptidase A 1XTF Neurotoxin BoNT/A E224Q Y366F mutant A 1XTG Crystal structure of neurotoxin BONT/A complexed with synaptosomal-associated protein 25 A 3BTA Crystal structure of botulinum neurotoxin serotype A B 1EPW Crystal Structure of Clostridium neurotoxin type B B 1F31 Crystal structure of Clostridium botulinum neurotoxin B complexed with a trisaccharide B 1F82 Botulinum neurotoxin type B catalytic domain B 1F83 Botulinum neurotoxin type B catalytic domain with synaptobrevin-II bound B 1G9A Crystal structure of Clostridium botulinum B complexed with an inhibitor (Experiment 3) B 1G9B Crystal structure of Clostridium bot
  • PDB identification refers to the 4 character code used by the Research Collaboratory for Structural Bioinformatics (RCSB) Protein Data Bank (http://www.rcsb.org/pdb/home/home.do) to identify a specific entry in the structural database.
  • RCSB Research Collaboratory for Structural Bioinformatics
  • Additional techniques employed include use of peptide/ antibody mapping information, for example, antibody mapping of sites on the surface of HC/A ( Dolimbek, BZ, 2007, Mol Immunol., 44(5):1029-41 ), HN/A ( Atassi MZ, 2004, Protein J. 23(1):39-52 ), H C /A ( Oshima M., 1998, Immunol Lett., 60(1):7-12 ; Bavari, S 1998, Vaccine, 16(19):1850-6 ), HC/E ( Kubota T, 1997, Appl Environ Microbiol.
  • the destructive cleavage site(s) are introduced at one or more of the following position(s), which are based (for convenience purposes) on the primary amino acid sequence of BoNT/A. Whilst the insertion positions are identified by reference to BoNT/A, the primary amino acid sequences of corresponding protease domains and/ or translocation domains for BoNT/B-G etc may be readily aligned with said BoNT/A positions-by way of example, we refer to the serotype alignment illustrated in Figure 2 .
  • one or more of the following positions is preferred: 27-31, 56-63, 73-75, 78-81, 99-105, 120-124, 137-144, 161-165, 169-173, 187-194, 202-214, 237-241, 243-250, 300-304, 323-335, 375-382, 391-400, and 413-423.
  • the above numbering preferably starts from the N-terminus of the protease component of the present invention.
  • the 99-105 and/ or 202-214 are most preferred.
  • positions 99-105 correspond to the sequence "YSTDLGR” for serotype A, which equates to the region "KSKPLGE” for serotype B, "NSREIGE” for serotype C 1 , "NERDIGK” for serotype D, "NNNLSGG” for serotype E, "NSNPAGQ” for serotype F, and "NSKPSGQ” for serotype G.
  • positions 202-214 correspond to the sequence "VDTNPLLGAGKFA" for serotype A, which equates to the region "NKGASIFNRRGYF” for serotype B, "DVGEGRFSKSEFC” for serotype C 1 , "NQSSAVLGKSIFC” for serotype D, "DNC----MN--EFI” for serotype E, "DN-TD--LFI” for serotype F, and "ENKDTSIFSRRAYF” for serotype G. and "P” (202) using the numbering at the top of Figure 2 as and "P", respectively.
  • the destructive cleavage site(s) are located at a position greater than 8 amino acid residues, preferably greater than 10 amino acid residues, more preferably greater than 25 amino acid residues, particularly preferably greater than 50 amino acid residues from the N-terminus of the protease component.
  • the destructive cleavage site(s) are located at a position greater than 20 amino acid residues, preferably greater than 30 amino acid residues, more preferably greater than 40 amino acid residues, particularly preferably greater than 50 amino acid residues from the C-terminus of the protease component.
  • one or more of the following positions is preferred: 474-479, 483-495, 507-543, 557-567, 576-580, 618-631, 643-650, 669-677, 751-767, 823-834, 845-859.
  • the above numbering preferably acknowledges a starting position of 449 for the N-terminus of the translocation domain component of the present invention, and a starting position of 871 for the C-terminus of the H N component. Of these positions, the 557-567 and/ or 751-767 are most preferred.
  • positions 557-567 correspond to the sequence "QEFEHGKSRIA” for serotype A, which equates to the region “QTFPLDIRDIS” for serotype B, "QKLSDNVEDFT” for serotype C 1 , “QKLSNNVENIT” for serotype D, “QKVPEGENNVN” for serotype E, “QKAPEGESAIS” for serotype F, and "QTFPSNIENLQ” for serotype G.
  • positions 751-767 correspond to the sequence "YNQYTEEEKNNINNID" for serotype A, which equates to the region "YNIYSEKEKSNIN--IDFN" for serotype B, "YKKYSGSDKENIKS--QVE” for serotype C 1 , “YKKYSGSDKENIKS--QVE” for serotype D, "YNSYTLEEKNELTNKYDIK” for serotype E, "YNNYTLDEKNRLRAEYNIY” for serotype F, and "YNRYSEEDKMNIN--IDFN" for serotype G.
  • the destructive cleavage site(s) are located at a position greater than 10 amino acid residues, preferably greater than 25 amino acid residues, more preferably greater than 40 amino acid residues, particularly preferably greater than 50 amino acid residues from the N-terminus of the translocation component.
  • the destructive cleavage site(s) are located at a position greater than 10 amino acid residues, preferably greater than 25 amino acid residues, more preferably greater than 40 amino acid residues, particularly preferably greater than 50 amino acid residues from the C-terminus of the translocation component.
  • a non-cytotoxic polypeptide for use in treating a range of diverse medical conditions and diseases.
  • Said conditions and diseases have established therapies (see the background part of the present specification) based on very closely related (though unmodified as per the present invention) non-cytotoxic polypeptides.
  • the present invention provides improvements to said therapies by use of a modified non-cytotoxic polypeptide that has a destructive cleavage site and thus reduced off-site effects.
  • the present invention provides polypeptides for use in the treatment of strabismus, blepharospasm, squint, spasmodic and oromandibular dystonia, torticollis, and other beauty therapy (cosmetic) applications benefiting from cell/ muscle incapacitation (via SNARE down-regulation or inactivation).
  • Additional, related therapies are provided for treating a neuromuscular disorder or condition of ocular motility, e.g. concomitant and vertical strabismus, lateral rectus palsy, nystagmus, dysthyroid myopathy, etc.; dystonia, e.g. focal dystonias such as spasmodic torticollis, writer's cramp, blepharospasm, oromandibular dystonia and the symptoms thereof, e.g. bruxism, Wilson's disease, tardive dystonia, laryngeal dystonia etc.; other dystonias, e.g.
  • a neuromuscular disorder or condition of ocular motility e.g. concomitant and vertical strabismus, lateral rectus palsy, nystagmus, dysthyroid myopathy, etc.
  • dystonia e.g. focal dystonias such as spasmodic torticollis, writer's cramp, blepharospas
  • spasms such as spasticity due to chronic multiple sclerosis, spasticity resulting in abnormal bladder control, e.g. in patients with spinal cord injury, animus, back spasm, charley horse etc.; tension headaches; levator pelvic syndrome; spina bifida, tardive dyskinesia; Parkinson's and limb (focal) dystonia and stuttering, etc.
  • a polypeptide of the invention binds to a surface structure (the Binding Site), which is present on and preferably characteristic of a target cell.
  • the polypeptide (at least the protease component thereof) becomes endocytosed into a vesicle, and the translocation component then directs transport of the protease component across the endosomal membrane and into the cytosol of the target cell.
  • the non-cytotoxic protease inhibits the cellular exocytic fusion process, and thereby inhibits release/ secretion from the target cell.
  • the biologically active component of the polypeptides of the present invention is a non-cytotoxic protease.
  • Non-cytotoxic proteases are a discrete class of molecules that do not kill cells; instead, they act by inhibiting cellular processes other than protein synthesis.
  • Non-cytotoxic proteases are produced as part of a larger toxin molecule by a variety of plants, and by a variety of microorganisms such as Clostridium sp. and Neisseria sp.
  • Clostridial neurotoxins represent a major group of non-cytotoxic toxin molecules, and comprise two polypeptide chains joined together by a disulphide bond.
  • the two chains are termed the heavy chain (H-chain), which has a molecular mass of approximately 100 kDa, and the light chain (L-chain), which has a molecular mass of approximately 50 kDa.
  • H-chain heavy chain
  • L-chain light chain
  • SNARE plasma membrane associated
  • Neisseria sp. most importantly from the species N. gonorrhoeae, produce functionally similar non-cytotoxic toxin molecules.
  • An example of such a non-cytotoxic protease is IgA protease (see WO99/58571 ).
  • TM determines the specificity of the polypeptide.
  • the same (or similar) receptor may be present on several different cells such that one TM will bind to different cell types. In this scenario, it might be desirable only to target a single cell type.
  • a second protease ('destruction') cleavage site in a polypeptide of the present invention which is cleaved by a protease specific to one or more of the undesired cells (and/ or to their environment), it is possible to minimise off-target side effects in the undesired cells.
  • polypeptides of the present invention may comprise two or more different TMs capable of binding to different target cell types.
  • combinations of polypeptides may be employed having different TMs so as to provide a coordinated targeting of different target cell types.
  • the polypeptides of the present invention comprise 4 principal components: a TM; a non-cytotoxic protease; a translocation domain; and a destructive protease cleavage site.
  • Said polypeptides embrace non-cytotoxic holotoxins such as clostridial neurotoxins, and, when an exogenous TM is present, re-targeted chimaeras (often referred to as re-targeted proteases).
  • the TM component of the present invention may be fused to either the protease component or the translocation component of the present invention.
  • Said fusion is preferably by way of a covalent bond, for example either a direct covalent bond or via a spacer/ linker molecule.
  • the protease component and the translocation component are preferably linked together via a covalent bond, for example either a direct covalent bond or via a spacer/ linker molecule.
  • Suitable spacer/ linked molecules are well known in the art, and typically comprise an amino acid-based sequence of between 5 and 40, preferably between 10 and 30 amino acid residues in length.
  • the polypeptides have a di-chain conformation, wherein the protease component and the translocation component are linked together, preferably via a disulphide bond.
  • polypeptides of the present invention may be prepared by conventional chemical conjugation techniques, which are well known to a skilled person.
  • chemical conjugation techniques such as Hermanson, G.T. (1996), Bioconjugate techniques, Academic Press , and to Wong, S.S. (1991), Chemistry of protein conjugation and cross-linking, CRC Press.
  • polypeptides may be prepared by recombinant preparation of a single polypeptide fusion protein (see, for example, WO98/07864 ).
  • This technique is based on the in vivo bacterial mechanism by which native clostridial neurotoxin (ie. holotoxin) is prepared, and results in a fusion protein having the following 'simplified' structural arrangement: NH 2 - [protease component] - [translocation component] - [TM] - COOH
  • the TM is placed towards the C-terminal end of the fusion protein.
  • the fusion protein is then 'activated' by treatment with a protease, which cleaves at a site between the protease component and the translocation component.
  • a di-chain protein is thus produced, comprising the protease component as a single polypeptide chain covalently attached (via a disulphide bridge) to another single polypeptide chain containing the translocation component plus TM.
  • the WO98/07864 system is particularly suited to the preparation of fusion proteins having a TM that requires a C-terminal domain that is 'free' for interaction with a Binding Site on a target cell.
  • a modified system may be employed as described in WO06/059113 .
  • the TM component of the fusion protein is located towards the middle of the linear fusion protein sequence, between the protease cleavage site and the translocation component. This ensures that the TM is attached to the translocation domain (ie. as occurs with native clostridial holotoxin), though in this case the two components are reversed in order vis-à-vis native holotoxin. Subsequent cleavage at the protease cleavage site exposes the N-terminal portion of the TM, and provides the di-chain polypeptide fusion protein.
  • protease cleavage sequence(s) may be introduced (and/ or any inherent cleavage sequence removed) at the DNA level by conventional means, such as by site-directed mutagenesis. Screening to confirm the presence of cleavage sequences may be performed manually or with the assistance of computer software (e.g. the MapDraw program by DNASTAR, Inc.). Whilst any protease cleavage site may be employed (ie.
  • clostridial or non-clostridial
  • the following are preferred (either as the 'destructive' cleavage site, or as the 'activation' cleavage site): Enterokinase (DDDDK ⁇ ) Factor Xa (IEGR ⁇ / IDGR ⁇ ) TEV(Tobacco Etch virus) (ENLYFQ ⁇ G) Thrombin (LVPR ⁇ GS) PreScission (LEVLFQ ⁇ GP).
  • protease cleavage site is an intein, which is a self-cleaving sequence.
  • the self-splicing reaction is controllable, for example by varying the concentration of reducing agent present.
  • the fusion protein of the present invention may comprise one or more N-terminal and/ or C-terminal located purification tags. Whilst any purification tag may be employed, the following are preferred:
  • One or more peptide spacer/ linker molecules may be included in the fusion protein.
  • a peptide spacer may be employed between a purification tag and the rest of the fusion protein molecule.
  • a third aspect of the present invention provides a nucleic acid (e.g. DNA) sequence encoding a polypeptide as described above.
  • Said nucleic acid may be included in the form of a vector, such as a plasmid, which may optionally include one or more of an origin of replication, a nucleic acid integration site, a promoter, a terminator, and a ribosome binding site.
  • a vector such as a plasmid, which may optionally include one or more of an origin of replication, a nucleic acid integration site, a promoter, a terminator, and a ribosome binding site.
  • the present disclosure also includes a method for expressing the above-described nucleic acid sequence (i.e. the third aspect of the present invention) in a host cell, in particular in E. coli or via a baculovirus expression system.
  • the present disclosure also includes a method for activating a polypeptide of the present invention, said method comprising contacting the polypeptide with a protease that cleaves the polypeptide at a recognition site (cleavage site) located between the non-cytotoxic protease component and the translocation component, thereby converting the polypeptide into a di-chain polypeptide wherein the non-cytotoxic protease and translocation components are joined together by a disulphide bond.
  • the recognition site is not native to a naturally-occurring clostridial neurotoxin and/ or to a naturally-occurring IgA protease.
  • the present invention employs a pharmaceutical composition, comprising a polypeptide, together with at least one component selected from a pharmaceutically acceptable carrier, excipient, adjuvant, propellant and/ or salt.
  • compositions suitable for injection may be in the form of solutions, suspensions or emulsions, or dry powders which are dissolved or suspended in a suitable vehicle prior to use.
  • the polypeptide may be formulated as a cream (eg. for topical application), or for sub-dermal injection.
  • Local delivery means may include an aerosol, or other spray (eg. a nebuliser).
  • an aerosol formulation of a polypeptide enables delivery to the lungs and/or other nasal and/or bronchial or airway passages.
  • Polypeptides of the invention may be administered to a patient by intrathecal or epidural injection in the spinal column at the level of the spinal segment involved in the innervation of an affected organ.
  • a preferred route of administration is via laproscopic and/ or localised, particularly intramuscular, injection.
  • a pharmaceutically active substance to assist retention at or reduce removal of the polypeptide from the site of administration.
  • a pharmaceutically active substance is a vasoconstrictor such as adrenaline.
  • Such a formulation confers the advantage of increasing the residence time of polypeptide following administration and thus increasing and/or enhancing its effect.
  • the dosage ranges for administration of the polypeptides of the present invention are those to produce the desired therapeutic effect. It will be appreciated that the dosage range required depends on the precise nature of the polypeptide or composition, the route of administration, the nature of the formulation, the age of the patient, the nature, extent or severity of the patient's condition, contraindications, if any, and the judgement of the attending physician. Variations in these dosage levels can be adjusted using standard empirical routines for optimisation.
  • Suitable daily dosages are in the range 0.0001-1 ng/kg, preferably 0.0001-0.5 ng/kg, more preferably 0.002-0.5 ng/kg, and particularly preferably 0.004-0.5 ng/kg.
  • the unit dosage can vary from less that 1 picogram to 30ng, but typically will be in the region of 0.01 to 1 ng per dose, which may be administered daily or preferably less frequently, such as weekly or six monthly.
  • a particularly preferred dosing regimen is based on 0.25 ng of polypeptide as the 1X dose.
  • preferred dosages are in the range 1X-100X (i.e. 0.25-25 ng).
  • Fluid dosage forms are typically prepared utilising the polypeptide and a pyrogen-free sterile vehicle.
  • the polypeptide depending on the vehicle and concentration used, can be either dissolved or suspended in the vehicle.
  • the polypeptide can be dissolved in the vehicle, the solution being made isotonic if necessary by addition of sodium chloride and sterilised by filtration through a sterile filter using aseptic techniques before filling into suitable sterile vials or ampoules and sealing.
  • solution stability is adequate, the solution in its sealed containers may be sterilised by autoclaving.
  • Advantageously additives such as buffering, solubilising, stabilising, preservative or bactericidal, suspending or emulsifying agents and or local anaesthetic agents may be dissolved in the vehicle.
  • Dry powders which are dissolved or suspended in a suitable vehicle prior to use, may be prepared by filling pre-sterilised ingredients into a sterile container using aseptic technique in a sterile area. Alternatively the ingredients may be dissolved into suitable containers using aseptic technique in a sterile area. The product is then freeze dried and the containers are sealed aseptically.
  • Parenteral suspensions suitable for intramuscular, subcutaneous or intradermal injection, are prepared in substantially the same manner, except that the sterile components are suspended in the sterile vehicle, instead of being dissolved and sterilisation cannot be accomplished by filtration.
  • the components may be isolated in a sterile state or alternatively it may be sterilised after isolation, e.g. by gamma irradiation.
  • a suspending agent for example polyvinylpyrrolidone is included in the composition/s to facilitate uniform distribution of the components.
  • Administration in accordance with the present invention may take advantage of a variety of delivery technologies including microparticle encapsulation, viral delivery systems or high-pressure aerosol impingement.
  • Targeting Moiety means any chemical structure that functionally interacts with a Binding Site to cause a physical association between the polypeptide of the invention and the surface of a target cell.
  • the term TM embraces any molecule (ie. a naturally occurring molecule, or a chemically/physically modified variant thereof) that is capable of binding to a Binding Site on the target cell, which Binding Site is capable of internalisation (eg. endosome formation) - also referred to as receptor-mediated endocytosis.
  • the TM may possess an endosomal membrane translocation function, in which case separate TM and Translocation Domain components need not be present in an agent of the present invention.
  • specific TMs have been described. Reference to said TMs is merely exemplary, and the present invention embraces all variants and derivatives thereof, which retain the basic binding (i.e. targeting) ability of the exemplified TMs.
  • preferred TMs include antibodies (eg. antibody fragments) and binding scaffolds; especially commercially available antibodies/ fragments and scaffolds designed for the purpose of binding (eg. specifically) to nerve cells.
  • Protein scaffolds represent a new generation of universal binding frameworks to complement the expanding repertoire of therapeutic monoclonal antibodies and derivatives such as scFvs, Fab molecules, dAbs (single-domain antibodies), diabodies and minibodies, each of which may be employed as a TM of the present invention.
  • Scaffold systems create or modify known protein recognition domains either through creation of novel scaffolds or modification of known protein binding domains.
  • Such scaffolds include but are not limited to:
  • Binding scaffolds can be used to target particular cell types via interaction with specific cell surface proteins, receptors or other cell surface epitopes such as sugar groups.
  • Such modified scaffolds can be engineered onto recombinant non-cytotoxic protease based polypeptides of the present invention to target specific nerve cell types of interest.
  • the TM of the present invention binds (preferably specifically binds) to the target cell in question.
  • the term "specifically binds” preferably means that a given TM binds to the target cell with a binding affinity (Ka) of 10 6 M -1 or greater, preferably 10 7 M -1 or greater, more preferably 10 8 M -1 or greater, and most preferably, 10 9 M -1 or greater.
  • TM in the present specification embraces fragments and variants thereof, which retain the ability to bind to the target cell in question.
  • a variant may have at least 80%, preferably at least 90%, more preferably at least 95%, and most preferably at least 97 or at least 99% amino acid sequence homology with the reference TM.
  • a variant may include one or more analogues of an amino acid (e.g. an unnatural amino acid), or a substituted linkage.
  • the term fragment when used in relation to a TM, means a peptide having at least ten, preferably at least twenty, more preferably at least thirty, and most preferably at least forty amino acid residues of the reference TM.
  • a fragment of the present invention may comprise a peptide sequence having at least 10, 20, 30 or 40 amino acids, wherein the peptide sequence has at least 80% sequence homology over a corresponding peptide sequence (of contiguous) amino acids of the reference peptide.
  • ErbB peptide TMs may be modified to generate mutein ErbB ligands with improved properties such as increased stability.
  • ErbB TM muteins include ErbB peptides having amino acid modifications such as a valine residue at position 46 or 47 (EGFVal46 or 47), which confers stability to cellular proteases.
  • ErbB TMs may also have amino acids deleted or additional amino acids inserted. This includes but is not limited to EGF having a deletion of the two C-terminal amino acids and a neutral amino acid substitution at position 51 (particularly EGF51Gln51; see US20020098178A1 ), and EGF with amino acids deleted (e.g.
  • Fragments of ErbB TMs may include fragments of TGF ⁇ which contain predicted ⁇ -turn regions (e.g. a peptide of the sequence Ac-C-H-S-G-Y-V-G-A-R-C-O-OMe), fragments of EGF such as [Ala20]EGF(14-31), and the peptide YHWYGYTPQNVI or GE11. All of the above patent specifications are incorporated herein by reference thereto.
  • TM binds to the selected target cell.
  • a simple radioactive displacement experiment may be employed in which tissue or cells representative of the target cell are exposed to labelled (eg. tritiated) TM in the presence of an excess of unlabelled TM.
  • the relative proportions of non-specific and specific binding may be assessed, thereby allowing confirmation that the TM binds to the target cell.
  • the assay may include one or more binding antagonists, and the assay may further comprise observing a loss of TM binding. Examples of this type of experiment can be found in Hulme, E.C. (1990), Receptor-binding studies, a brief outline, pp. 303-311, In Receptor biochemistry, A Practical Approach, Ed. E.C. Hulme, Oxford University Press .
  • peptide TM embraces peptide analogues thereof, so long as the analogue binds to the same receptor as the corresponding 'reference' TM.
  • Said analogues may include synthetic residues such as:
  • the polypeptides of the present invention may lack a functional H C (or H CC ) domain of a clostridial neurotoxin, in which case a non-clostridial TM is typically present to bind the polypeptide to a Binding Site on the nerve cell.
  • the H C peptide of a native clostridial neurotoxin comprises approximately 400-440 amino acid residues, and consists of two functionally distinct domains of approximately 25kDa each, namely the N-terminal region (commonly referred to as the H CN peptide or domain) and the C-terminal region (commonly referred to as the H CC peptide or domain).
  • H CC C-terminal region
  • a polypeptide of the present invention may contain a functional H C (or Hcc) domain of a clostridial neurotoxin as a TM.
  • Hcc clostridial neurotoxin
  • a variety of clostridial neurotoxin Hcc or H C regions comprising a binding domain can be useful in aspects of the present invention with the proviso that these active fragments provide the binding activity and binding specificity of the natural neurotoxin.
  • the H C regions from the heavy chains of clostridial toxins are approximately 400-440 amino acids in length and comprise a binding domain. Research has shown that the entire length of a H C region from a clostridial toxin heavy chain is not necessary for the binding activity of the binding domain.
  • aspects of this embodiment can include clostridial toxin H C regions comprising a binding domain having a length of, for example, at least 350 amino acids, at least 375 amino acids, at least 400 amino acids and at least 425 amino acids.
  • Other aspects of this embodiment can include clostridial toxin H C regions comprising a binding domain having a length of, for example, at most 350 amino acids, at most 375 amino acids, at most 400 amino acids and at most 425 amino acids.
  • the protease of the present invention embraces all non-cytotoxic proteases that are capable of cleaving one or more proteins of the exocytic fusion apparatus in eukaryotic cells.
  • the protease of the present invention is preferably a bacterial protease (or fragment thereof). More preferably the bacterial protease is selected from the genera Clostridium or Neisseria / Streptococcus (e.g. a clostridial L-chain, or a neisserial IgA protease preferably from N. gonorrhoeae or S. pneumoniae ).
  • the present invention also embraces variant non-cytotoxic proteases (ie. variants of naturally-occurring protease molecules), so long as the variant proteases still demonstrate the requisite protease activity.
  • a variant may have at least 70%, preferably at least 80%, more preferably at least 90%, and most preferably at least 95 or at least 98% amino acid sequence homology with a reference protease sequence.
  • the term variant includes non-cytotoxic proteases having enhanced (or decreased) endopeptidase activity - particular mention here is made to the increased K cat /K m of BoNT/A mutants Q161A, E54A, and K165L see Ahmed, S.A. (2008) Protein J.
  • fragment when used in relation to a protease, typically means a peptide having at least 150, preferably at least 200, more preferably at least 250, and most preferably at least 300 amino acid residues of the reference protease.
  • protease 'fragments' of the present invention embrace fragments of variant proteases based on a reference sequence.
  • the protease of the present invention preferably demonstrates a serine or metalloprotease activity (e.g. endopeptidase activity).
  • the protease is preferably specific for a SNARE protein (e.g. SNAP-25, synaptobrevin/VAMP, or syntaxin).
  • protease domains of neurotoxins for example the protease domains of bacterial neurotoxins.
  • the present invention embraces the use of neurotoxin domains, which occur in nature, as well as recombinantly prepared versions of said naturally-occurring neurotoxins.
  • Exemplary neurotoxins are produced by clostridia, and the term clostridial neurotoxin embraces neurotoxins produced by C. tetani (TeNT), and by C. botulinum (BoNT) serotypes A-G, as well as the closely related BoNT-like neurotoxins produced by C. baratii and C. butyricum.
  • TeNT C. tetani
  • BoNT botulinum
  • BoNT/A denotes the source of neurotoxin as BoNT (serotype A).
  • Corresponding nomenclature applies to other BoNT serotypes.
  • BoNTs are the most potent toxins known, with median lethal dose (LD50) values for mice ranging from 0.5 to 5 ng/kg depending on the serotype. BoNTs are adsorbed in the gastrointestinal tract, and, after entering the general circulation, bind to the presynaptic membrane of cholinergic nerve terminals and prevent the release of their neurotransmitter acetylcholine.
  • BoNT/B, BoNT/D, BoNT/F and BoNT/G cleave synaptobrevin/vesicle-associated membrane protein (VAMP);
  • VAMP synaptobrevin/vesicle-associated membrane protein
  • BoNT/C, BoNT/A and BoNT/E cleave the synaptosomal-associated protein of 25 kDa (SNAP-25); and BoNT/C cleaves syntaxin.
  • BoNTs share a common structure, being di-chain proteins of ⁇ 150 kDa, consisting of a heavy chain (H-chain) of 100 kDa covalently joined by a single disulphide bond to a light chain (L-chain) of ⁇ 50 kDa.
  • the H-chain consists of two domains, each of ⁇ 50 kDa.
  • the C-terminal domain (H C ) is required for the high-affinity neuronal binding, whereas the N-terminal domain (H N ) is proposed to be involved in membrane translocation.
  • the L-chain is a zinc-dependent metalloprotease responsible for the cleavage of the substrate SNARE protein.
  • L-chain fragment means a component of the L-chain of a neurotoxin, which fragment demonstrates a metalloprotease activity and is capable of proteolytically cleaving a vesicle and/or plasma membrane associated protein involved in cellular exocytosis.
  • protease (reference) sequences include: Botulinum type A neurotoxin - amino acid residues (1-448) Botulinum type B neurotoxin - amino acid residues (1-440) Botulinum type C neurotoxin - amino acid residues (1-441) Botulinum type D neurotoxin - amino acid residues (1-445) Botulinum type E neurotoxin - amino acid residues (1-422) Botulinum type F neurotoxin - amino acid residues (1-439) Botulinum type G neurotoxin - amino acid residues (1-441) Tetanus neurotoxin - amino acid residues (1-457) IgA protease - amino acid residues (1-959)* * Pohlner, J. et al. (1987). Nature 325, pp. 458-462 .
  • a variety of clostridial toxin fragments comprising the light chain can be useful in aspects of the present invention with the proviso that these light chain fragments can specifically target the core components of the neurotransmitter release apparatus and thus participate in executing the overall cellular mechanism whereby a clostridial toxin proteolytically cleaves a substrate.
  • the light chains of clostridial toxins are approximately 420-460 amino acids in length and comprise an enzymatic domain. Research has shown that the entire length of a clostridial toxin light chain is not necessary for the enzymatic activity of the enzymatic domain. As a non-limiting example, the first eight amino acids of the BoNT/A light chain are not required for enzymatic activity.
  • the first eight amino acids of the TeNT light chain are not required for enzymatic activity.
  • the carboxyl-terminus of the light chain is not necessary for activity.
  • the last 32 amino acids of the BoNT/A light chain are not required for enzymatic activity.
  • the last 31 amino acids of the TeNT light chain are not required for enzymatic activity.
  • aspects of this embodiment can include clostridial toxin light chains comprising an enzymatic domain having a length of, for example, at least 350 amino acids, at least 375 amino acids, at least 400 amino acids, at least 425 amino acids and at least 450 amino acids.
  • Other aspects of this embodiment can include clostridial toxin light chains comprising an enzymatic domain having a length of, for example, at most 350 amino acids, at most 375 amino acids, at most 400 amino acids, at most 425 amino acids and at most 450 amino acids.
  • the polypeptides of the present invention may be PEGylated - this may help to increase stability, for example duration of action of the protease component.
  • PEGylation is particularly preferred when the protease comprises a BoNT/A, B or C 1 protease.
  • PEGylation preferably includes the addition of PEG to the N-terminus of the protease component.
  • the N-terminus of a protease may be extended with one or more amino acid (e.g. cysteine) residues, which may be the same or different.
  • One or more of said amino acid residues may have its own PEG molecule attached (e.g. covalently attached) thereto.
  • An example of this technology is described in WO2007/104567 , which is incorporated in its entirety by reference thereto.
  • a Translocation Domain is a molecule that enables translocation of a protease into a target cell such that a functional expression of protease activity occurs within the cytosol of the target cell. Whether any molecule (e.g. a protein or peptide) possesses the requisite translocation function of the present invention may be confirmed by any one of a number of conventional assays.
  • Shone C. (1987) describes an in vitro assay employing liposomes, which are challenged with a test molecule. Presence of the requisite translocation function is confirmed by release from the liposomes of K + and/ or labelled NAD, which may be readily monitored [see Shone C. (1987) Eur. J. Biochem; vol. 167(1): pp. 175-180 ].
  • Blaustein R. (1987) describes a simple in vitro assay employing planar phospholipid bilayer membranes. The membranes are challenged with a test molecule and the requisite translocation function is confirmed by an increase in conductance across said membranes [see Blaustein (1987) FEBS Letts; vol. 226, no. 1: pp. 115-120 ].
  • a variant may have at least 70%, preferably at least 80%, more preferably at least 90%, and most preferably at least 95% or at least 98% amino acid sequence homology with a reference translocation domain.
  • the term fragment when used in relation to a translocation domain, means a peptide having at least 20, preferably at least 40, more preferably at least 80, and most preferably at least 100 amino acid residues of the reference translocation domain.
  • the fragment preferably has at least 100, preferably at least 150, more preferably at least 200, and most preferably at least 250 amino acid residues of the reference translocation domain (eg. H N domain).
  • the reference translocation domain eg. H N domain.
  • translocation 'fragments' of the present invention embrace fragments of variant translocation domains based on the reference sequences.
  • the Translocation Domain is preferably capable of formation of ion-permeable pores in lipid membranes under conditions of low pH. Preferably it has been found to use only those portions of the protein molecule capable of pore-formation within the endosomal membrane.
  • the Translocation Domain may be obtained from a microbial protein source, in particular from a bacterial or viral protein source.
  • the Translocation Domain is a translocating domain of an enzyme, such as a bacterial toxin or viral protein.
  • the Translocation Domain may be of a clostridial origin, such as the H N domain (or a functional component thereof).
  • H N means a portion or fragment of the H-chain of a clostridial neurotoxin approximately equivalent to the amino-terminal half of the H-chain, or the domain corresponding to that fragment in the intact H-chain.
  • the H-chain lacks the natural binding function of the H C component of the H-chain.
  • the H C function may be removed by deletion of the H C amino acid sequence (either at the DNA synthesis level, or at the post-synthesis level by nuclease or protease treatment). Alternatively, the H C function may be inactivated by chemical or biological treatment.
  • the H-chain is incapable of binding to the Binding Site on a target cell to which native clostridial neurotoxin (i.e. holotoxin) binds.
  • Suitable (reference) Translocation Domains include: Botulinum type A neurotoxin - amino acid residues (449-871) Botulinum type B neurotoxin - amino acid residues (441-858) Botulinum type C neurotoxin - amino acid residues (442-866) Botulinum type D neurotoxin - amino acid residues (446-862) Botulinum type E neurotoxin - amino acid residues (423-845) Botulinum type F neurotoxin - amino acid residues (440-864) Botulinum type G neurotoxin - amino acid residues (442-863) Tetanus neurotoxin - amino acid residues (458-879)
  • Clostridial toxin H N regions comprising a translocation domain can be useful in aspects of the present invention with the proviso that these active fragments can facilitate the release of a non-cytotoxic protease (e.g. a clostridial L-chain) from intracellular vesicles into the cytoplasm of the target cell and thus participate in executing the overall cellular mechanism whereby a clostridial toxin proteolytically cleaves a substrate.
  • the H N regions from the heavy chains of Clostridial toxins are approximately 410-430 amino acids in length and comprise a translocation domain.
  • aspects of this embodiment can include clostridial toxin H N regions comprising a translocation domain having a length of, for example, at least 350 amino acids, at least 375 amino acids, at least 400 amino acids and at least 425 amino acids.
  • Other aspects of this embodiment can include clostridial toxin H N regions comprising translocation domain having a length of, for example, at most 350 amino acids, at most 375 amino acids, at most 400 amino acids and at most 425 amino acids.
  • H N embraces naturally-occurring neurotoxin H N portions, and modified H N portions having amino acid sequences that do not occur in nature and/ or synthetic amino acid residues, so long as the modified H N portions still demonstrate the above-mentioned translocation function.
  • the Translocation Domain may be of a non-clostridial origin.
  • the Translocation Domain may mirror the Translocation Domain present in a naturally-occurring protein, or may include amino acid variations so long as the variations do not destroy the translocating ability of the Translocation Domain.
  • viral (reference) Translocation Domains suitable for use in the present invention include certain translocating domains of virally expressed membrane fusion proteins.
  • translocation i.e. membrane fusion and vesiculation
  • the translocation i.e. membrane fusion and vesiculation function of a number of fusogenic and amphiphilic peptides derived from the N-terminal region of influenza virus haemagglutinin.
  • virally expressed membrane fusion proteins known to have the desired translocating activity are a translocating domain of a fusogenic peptide of Semliki Forest Virus (SFV), a translocating domain of vesicular stomatitis virus (VSV) glycoprotein G, a translocating domain of SER virus F protein and a translocating domain of Foamy virus envelope glycoprotein.
  • SFV Semliki Forest Virus
  • VSV vesicular stomatitis virus
  • SER virus F protein a translocating domain of Foamy virus envelope glycoprotein.
  • Virally encoded Aspike proteins have particular application in the context of the present invention, for example, the E1 protein of SFV and the G protein of the G protein of VSV.
  • Translocation Domains listed in Table (below) includes use of sequence variants thereof.
  • a variant may comprise one or more conservative nucleic acid substitutions and/ or nucleic acid deletions or insertions, with the proviso that the variant possesses the requisite translocating function.
  • a variant may also comprise one or more amino acid substitutions and/ or amino acid deletions or insertions, so long as the variant possesses the requisite translocating function.
  • Translocation Domain source Amino acid residues References Diphtheria toxin 194-380 Silverman et al., 1994, J. Biol. Chem. 269, 22524-22532 London E., 1992, Biochem. Biophys.
  • the polypeptides of the present invention may further comprise a translocation facilitating domain.
  • Said domain facilitates delivery of the non-cytotoxic protease into the cytosol of the target cell and are described, for example, in WO 08/008803 and WO 08/008805 .
  • suitable translocation facilitating domains include an enveloped virus fusogenic peptide domain
  • suitable fusogenic peptide domains include influenzavirus fusogenic peptide domain (eg. influenza A virus fusogenic peptide domain of 23 amino acids), alphavirus fusogenic peptide domain (eg. Semliki Forest virus fusogenic peptide domain of 26 amino acids), vesiculovirus fusogenic peptide domain (eg. vesicular stomatitis virus fusogenic peptide domain of 21 amino acids), respirovirus fusogenic peptide domain (eg. Sendai virus fusogenic peptide domain of 25 amino acids), morbiliivirus fusogenic peptide domain (eg.
  • influenza virus fusogenic peptide domain eg. influenza A virus fusogenic peptide domain of 23 amino acids
  • alphavirus fusogenic peptide domain eg. Semliki Forest virus fusogenic peptide domain of 26 amino acids
  • Canine distemper virus fusogenic peptide domain of 25 amino acids canine distemper virus fusogenic peptide domain of 25 amino acids
  • avulavirus fusogenic peptide domain eg. Newcastle disease virus fusogenic peptide domain of 25 amino acids
  • henipavirus fusogenic peptide domain eg. Hendra virus fusogenic peptide domain of 25 amino acids
  • metapneumovirus fusogenic peptide domain eg. Human metapneumovirus fusogenic peptide domain of 25 amino acids
  • spumavirus fusogenic peptide domain such as simian foamy virus fusogenic peptide domain; or fragments or variants thereof.
  • a translocation facilitating domain may comprise a Clostridial toxin H CN domain or a fragment or variant thereof.
  • a Clostridial toxin H CN translocation facilitating domain may have a length of at least 200 amino acids, at least 225 amino acids, at least 250 amino acids, at least 275 amino acids.
  • a Clostridial toxin H CN translocation facilitating domain preferably has a length of at most 200 amino acids, at most 225 amino acids, at most 250 amino acids, or at most 275 amino acids.
  • Botulinum type A neurotoxin - amino acid residues (872-1110) Botulinum type B neurotoxin - amino acid residues (859-1097)
  • Botulinum type G neurotoxin - amino acid residues (864-1105) Tetanus neurotoxin - amino acid residues (880-1127)
  • Clostridial toxin H CN domains include: Botulinum type A neurotoxin - amino acid residues (874-1110) Botulinum type B neurotoxin - amino acid residues (861-1097) Botulinum type C neurotoxin - amino acid residues (869-1111) Botulinum type D neurotoxin - amino acid residues (865-1098) Botulinum type E neurotoxin - amino acid residues (848-1085) Botulinum type F neurotoxin - amino acid residues (867-1105) Botulinum type G neurotoxin - amino acid residues (866-1105) Tetanus neurotoxin - amino acid residues (882-1127)
  • any of the above-described facilitating domains may be combined with any of the previously described translocation domain peptides that are suitable for use in the present invention.
  • a non-clostridial facilitating domain may be combined with non-clostridial translocation domain peptide or with clostridial translocation domain peptide.
  • a Clostridial toxin H CN translocation facilitating domain may be combined with a non-clostridial translocation domain peptide.
  • a Clostridial toxin H CN facilitating domain may be combined or with a clostridial translocation domain peptide, examples of which include: Botulinum type A neurotoxin - amino acid residues (449-1110) Botulinum type B neurotoxin - amino acid residues (442-1097) Botulinum type C neurotoxin - amino acid residues (450-1111) Botulinum type D neurotoxin - amino acid residues (446-1098) Botulinum type E neurotoxin - amino acid residues (423-1085) Botulinum type F neurotoxin - amino acid residues (440-1105) Botulinum type G neurotoxin - amino acid residues (447-1105) Tetanus neurotoxin - amino acid residues (458-1127)
  • sequence alignment methods can be used to determine percent identity, including, without limitation, global methods, local methods and hybrid methods, such as, e.g., segment approach methods. Protocols to determine percent identity are routine procedures within the scope of one skilled in the.
  • Global methods align sequences from the beginning to the end of the molecule and determine the best alignment by adding up scores of individual residue pairs and by imposing gap penalties.
  • Non-limiting methods include, e.g., CLUSTAL W, see, e.g., Julie D.
  • Non-limiting methods include, e.g., Match-box, see, e.g., Eric Depiereux and Ernest Feytmans, Match-Box: A Fundamentally New Algorithm for the Simultaneous Alignment of Several Protein Sequences, 8(5) CABIOS 501 -509 (1992 ); Gibbs sampling, see, e.g., C. E.
  • percent sequence identity is determined by conventional methods. See, for example, Altschul et al., Bull. Math. Bio. 48: 603-16, 1986 and Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915-19, 1992 . Briefly, two amino acid sequences are aligned to optimize the alignment scores using a gap opening penalty of 10, a gap extension penalty of 1, and the "blosum 62" scoring matrix of Henikoff and Henikoff (ibid.) as shown below (amino acids are indicated by the standard one-letter codes).
  • the percent identity is then calculated as: Total number of identical matches [ length of the longer sequence plus the number of gaps introduced into the longer sequence in order to align the two sequences ] ⁇ 100
  • Substantially homologous polypeptides are characterized as having one or more amino acid substitutions, deletions or additions. These changes are preferably of a minor nature, that is conservative amino acid substitutions (see below) and other substitutions that do not significantly affect the folding or activity of the polypeptide; small deletions, typically of one to about 30 amino acids; and small amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue, a small linker peptide of up to about 20-25 residues, or an affinity tag.
  • non-standard amino acids such as 4-hydroxyproline, 6- N -methyl lysine, 2-aminoisobutyric acid, isovaline and ⁇ -methyl serine
  • a limited number of non-conservative amino acids, amino acids that are not encoded by the genetic code, and unnatural amino acids may be substituted for clostridial polypeptide amino acid residues.
  • the polypeptides of the present invention can also comprise non-naturally occurring amino acid residues.
  • Non-naturally occurring amino acids include, without limitation, trans-3-methylproline, 2,4-methano-proline, cis-4-hydroxyproline, trans-4-hydroxy-proline, N-methylglycine, allo-threonine, methyl-threonine, hydroxy-ethylcysteine, hydroxyethylhomo-cysteine, nitro-glutamine, homoglutamine, pipecolic acid, tert-leucine, norvaline, 2-azaphenylalanine, 3-azaphenyl-alanine, 4-azaphenyl-alanine, and 4-fluorophenylalanine.
  • Several methods are known in the art for incorporating non-naturally occurring amino acid residues into proteins.
  • an in vitro system can be employed wherein nonsense mutations are suppressed using chemically aminoacylated suppressor tRNAs.
  • Methods for synthesizing amino acids and aminoacylating tRNA are known in the art. Transcription and translation of plasmids containing nonsense mutations is carried out in a cell free system comprising an E. coli S30 extract and commercially available enzymes and other reagents. Proteins are purified by chromatography. See, for example, Robertson et al., J. Am. Chem. Soc. 113:2722, 1991 ; Ellman et al., Methods Enzymol.
  • coli cells are cultured in the absence of a natural amino acid that is to be replaced (e.g., phenylalanine) and in the presence of the desired non-naturally occurring amino acid(s) (e.g., 2-azaphenylalanine, 3-azaphenylalanine, 4-azaphenylalanine, or 4-fluorophenylalanine).
  • the non-naturally occurring amino acid is incorporated into the polypeptide in place of its natural counterpart. See, Koide et al., Biochem. 33:7470-6, 1994 .
  • Naturally occurring amino acid residues can be converted to non-naturally occurring species by in vitro chemical modification. Chemical modification can be combined with site-directed mutagenesis to further expand the range of substitutions ( Wynn and Richards, Protein Sci. 2:395-403, 1993 ).
  • a limited number of non-conservative amino acids, amino acids that are not encoded by the genetic code, non-naturally occurring amino acids, and unnatural amino acids may be substituted for amino acid residues of polypeptides of the present invention.
  • Essential amino acids in the polypeptides of the present invention can be identified according to procedures known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis ( Cunningham and Wells, Science 244: 1081-5, 1989 ). Sites of biological interaction can also be determined by physical analysis of structure, as determined by such techniques as nuclear magnetic resonance, crystallography, electron diffraction or photoaffinity labeling, in conjunction with mutation of putative contact site amino acids. See, for example, de Vos et al., Science 255:306-12, 1992 ; Smith et al., J. Mol. Biol. 224:899-904, 1992 ; Wlodaver et al., FEBS Lett. 309:59-64, 1992 . The identities of essential amino acids can also be inferred from analysis of homologies with related components (e.g. the translocation or protease components) of the polypeptides of the present invention.
  • related components e.g. the trans
  • Example 1 Assessment of polypeptides of the invention when exposed to a mammalian cell (muscle).
  • Example 2 Assessment of polypeptides of the invention when exposed to a mammalian cell having first exposed the polypeptide to circulatory proteases.
  • Example 3 Assessment of the catalytic activity of polypeptides of the invention.
  • Example 4 Assessment of the translocation ability of polypeptides of the invention.
  • Example 5 Creation of an LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the LC.
  • Example 6 Purification of an LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the LC.
  • Example 7 Demonstration of enhanced protease sensitivity in an LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the LC.
  • Example 8 Creation of an LHC-EGF chimaeric protein that incorporates a Thrombin recognition site into the LC.
  • Example 9 Creation of an LHA-EGF chimaeric protein that incorporates a Thrombin recognition site into the LC.
  • Example 9 Creation of an LHA-EGF chimaeric protein that incorporates a Thrombin recognition site into the LC.
  • Example 10 Creation of an LHC-EGF chimaeric protein that incorporates a furin recognition site into the LC.
  • Example 11 Creation of an LHA-EGF chimaeric protein that incorporates a Factor Xa recognition site into the H N domain.
  • Example 12 Creation of a LHA-EGF chimaeric protein that incorporates an ADAM17 recognition site into the LC domain.
  • Example 14 Creation of a recombinant BoNT/A protein that incorporates a furin recognition site into the H N .
  • Example 15 Treatment of a patient suffering from dystonia (Spasmodic Torticollis).
  • Example 16 Treatment of a patient suffering from blepharospasm.
  • Example 17 Creation of a LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the LC at position 210
  • Example 18 Creation of a LHC-EGF chimaeric protein that incorporates a Thrombin recognition site into the LC at position 195
  • Example 19 Creation of a LHC-EGF chimaeric protein that incorporates a Thrombin recognition site into the LC at position 210
  • Example 20 Creation of a LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the H N domain at position 742 of the H N
  • Example 21 Creation of a LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the H N domain at position 750 of the H N
  • Example 22 Creation of a LHC-EGF chimaeric protein that incorporates a Thrombin recognition site into the H N domain at position 750 of the H N
  • Example 23 Creation of a LHD-VIPr chimaeric protein that incorporates a Factor Xa recognition site into the H N domain at position 798 of the H N
  • Example 24 Creation of an LHA-EGF chimaeric protein that incorporates a Thrombin recognition site into the LC domain
  • Example 25 Demonstration of specific cleavage of a purified LHC-EGF chimaeric protein that is engineered to incorporate a Factor Xa recognition site into the LC.
  • Example 26 Demonstration of specific cleavage of a purified LHC-EGF chimaeric protein that is engineered to incorporate a Factor Xa recognition site into the H N .
  • Example 27 Demonstration of specific cleavage of a purified LHC-EGF chimaeric protein that is engineered to incorporate a Thrombin recognition site into the LC
  • Example 28 Demonstration of specific cleavage of a purified LHA-EGF chimaeric protein that is engineered to incorporate a Thrombin recognition site into the LC
  • Example 29 Demonstration of reduced in vitro cellular activity of a protein engineered to incorporate a Factor Xa protease cleavage site into the LC domain of L(FXa)HC-EGF
  • Example 30 Demonstration of reduced in vitro cellular activity of a protein engineered to incorporate a Thrombin protease cleavage site into the LC domain of L(Thr)HA-EGF
  • Example 32 Creation of a recombinant BoNT/A protein that incorporates a Factor Xa recognition site into the LC.
  • Example 35 Creation of a recombinant BoNT/E protein that incorporates a Factor Xa recognition site into the H N .
  • Example 36 Creation of an LHE-VIPr chimaeric protein that incorporates a Thrombin recognition site into the LC.
  • Example 37 Creation of an LHE-VIPr chimaeric protein that incorporates a Factor Xa recognition site into the H N .
  • Example 38 Creation of an LHE-VIPr chimaeric protein that incorporates a Factor Xa recognition site into the LC.
  • Example 39 Cleavage of SNARE protein by a modified clostridial neurotoxin (LH N ) having the properties described by LIN, et al. (WO02/044199 )
  • Example 1 Assessment of polypeptides of the invention when exposed to a mammalian muscle cell
  • a purified protein created according to Example 13 is incubated in the presence of a mammalian muscle cell (coronary smooth muscle primary culture or HSkMC (150-05f) cell (available from ECACC)).
  • a second polypeptide is incubated under identical conditions in the presence of the same test cell-type.
  • Each of the two polypeptides is then assessed for cleavage by ADAM 17 (inherent to the coronary smooth muscle primary culture/ HSkMC cell) by SDS-PAGE and subsequent Western blot analysis.
  • ADAM 17 inherent to the coronary smooth muscle primary culture/ HSkMC cell
  • SDS-PAGE subsequent to the Western blot analysis.
  • a greater observed cleavage for the first polypeptide versus that observed for the second polypeptide confirms controllable inactivation of the present invention.
  • Example 2 Assessment of polypeptides of the invention when exposed to a mammalian cell having first exposed the polypeptide to a circulatory protease
  • a first polypeptide (SEQ ID 4); prepared according to Example 5 of the present invention) is taken and incubated in the presence of a target cell having first exposed the polypeptide to circulatory proteases (for example, Factor Xa, Thrombin) in vitro.
  • a second polypeptide (SEQ ID2; identical to the first polypeptide other than for the fact that it lacks the protease cleavage site) is incubated in the same manner as for the first polypeptide.
  • Each of the two polypeptides is then assessed for cleavage of syntaxin in a non-human embryonic spinal cord neuron (eSCN).
  • eSCN non-human embryonic spinal cord neuron
  • a first polypeptide (SEQ ID 10; prepared according to Example 9 of the present invention) is incubated in vitro in the presence of a protease (thrombin) that cleaves the polypeptide at a destructive cleavage site introduced into the protease domain of the polypeptide.
  • a second polypeptide (SEQ ID 8: identical to the first polypeptide other than for the fact that it lacks the protease cleavage site) is incubated in an identical manner in the presence of the same protease.
  • Each of the two polypeptides is then challenged in an in vitro cell-free system (as described by Hallis et al 1996, J. Clin. Microbiol. 34 1934-1938 ) containing immobilised SNAP-25, and cleavage of SNAP-25 protein is measured by using specific antisera raised to the cleavage product.
  • a lesser observed SNARE protein cleavage for the first polypeptide versus that observed for the second polypeptide confirms controllable inactivation of the present invention.
  • a first polypeptide (according to the present invention) is incubated in the presence of a protease that cleaves the polypeptide at a destructive cleavage site introduced into the translocation (e.g. H N ) domain.
  • a second polypeptide (identical to the first polypeptide other than for the fact that it lacks the protease cleavage site) is incubated in an identical manner in the presence of the same protease.
  • each of the two polypeptides is then challenged in an in vitro system containing a lipid bilayer membrane, and transport across the membrane is measured.
  • Shone C. (1987) describes an in vitro assay employing liposomes, which are challenged with a test molecule. Presence of the requisite translocation function is confirmed by release from the liposomes of K+ and/or labelled NAD, which may be readily monitored [see Shone C. (1987) Eur. J. Biochem; vol. 167(1): pp. 175-180 ].
  • a further example is provided by Blaustein R. (1987), which describes a simple in vitro assay employing planar phospholipid bilayer membranes. The membranes are challenged with a test molecule and the requisite translocation function is confirmed by an increase in conductance across said membranes [see Blaustein (1987) FEBS Letts; vol. 226, no. 1: pp. 115-120 ].
  • This method is applied to study the protease inactivation of the H N domain of serotype D BoNT.
  • the protein of Example 23 is expressed and purified and is exposed to Factor Xa to result in cleavage of the protein within the H N domain.
  • the cleaved protein is assessed in the in vitro system described above and compared to the protein that has not been treated with Factor Xa. The experiment determines that the transport across the membrane for the Factor Xa-treated polypeptide is significantly less than that of the untreated polypeptide.
  • Example 5 Creation of an LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the LC.
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/C and the human epidermal growth factor sequence (SEQ ID 2) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Factor Xa (IEGR).
  • Simple text character analysis of the primary sequence identified the sequence 210 GEGR 213 within the LC domain.
  • the location of the peptide in the tertiary structure of the LC/C is predicted from examination of the location of the homologous peptide sequence in the LC/A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • SEQ ID 1 Site directed mutagenesis of the SEQ ID 1 (encoding SEQ ID 2) using a primer designed to switch the codon for G210 (GGC) to one that encodes Ile (ATC) was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage was assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA was incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA was checked by sequencing.
  • the final ORF incorporating the Factor Xa site is illustrated as SEQ ID 3 and the amino acid sequence of the expression product is illustrated in SEQ ID 4.
  • Example 6 Purification of an LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the LC
  • the ORF created in Example 17 was cloned into an E. coli expression vector (a pET (Novagen) vector that has been modified to ensure mobilisation deficiency) and transformed into an E. coli host strain, most commonly BL21.
  • the vector was modified to include expression of a Histidine tag at the N-terminus of the LHC-EGF ORF.
  • LHC-EGF fusion protein Expression of the LHC-EGF fusion protein is achieved using the following protocol. Inoculate 100 ml of modified TB containing 0.2% glucose and 100 ⁇ g/ml ampicillin in a 250 ml flask with a single colony from the LHC-EGF expression strain. Grow the culture at 37°C, 225 rpm for 16 hours. Inoculate 1L of modified TB containing 0.2% glucose and 100 ⁇ g/ml ampicillin in a 2L flask with 10ml of overnight culture. Grow cultures at 37°C until an approximate OD600nm of 0.5 is reached at which point reduce the temperature to 16°C. After 1 hour induce the cultures with 1 mM IPTG and grow at 16°C for a further 16 hours.
  • Example 7 Demonstration of enhanced protease sensitivity in an LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the LC
  • the purified chimaeric protein of Example 6 is assessed for its stability in the presence of protease using the methodology outlines in Example 2 and 3.
  • the LHC-EGF chimaeric protein is exposed to a range of concentrations of Factor Xa protease (obtained, for example, from New England Biolabs #P8010L) in vitro over a period of 1-120 minutes.
  • the proteolysis is terminated by addition of a specific inhibitor of Factor Xa (for example dansyl-glu-gly-arg-chloromethyl ketone (CALBIOCHEM, #251700)).
  • a specific inhibitor of Factor Xa for example Dansyl-glu-gly-arg-chloromethyl ketone (CALBIOCHEM, #251700).
  • a control protein chimaera of LHC-EGF that does not include the additional Factor Xa site is used to compare the effect of the protease on LC activity (using Example 3), and functionality of the chimaera when exposed to a target cell (using Example 2 and measuring syntaxin cleavage in a non-human embryonic spinal cord neuron (eSCN)).
  • Example 8 Creation of an LHC-EGF chimaeric protein that incorporates a Thrombin recognition site into the LC
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/C and the human epidermal growth factor sequence (SEQ ID 2) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Thrombin (LVPRGS).
  • Simple text character analysis of the primary sequence identified the sequence 194 ISPRFM 199 within the LC domain.
  • the location of the peptide in the tertiary structure of the LC/C is predicted from examination of the location of the homologous peptide sequence in the LC/A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 1 (encoding SEQ ID 2) using a primer designed to switch the codons for S 195 to Val (TCT to GTT) and M 195 to Ser (ATG to TCC) changes the region 194 ISPRFM 199 to IVPRFS to make it a substrate for Thrombin cleavage. Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Thrombin site is illustrated as SEQ ID 5 and the amino acid sequence of the expression product is illustrated in SEQ ID 6.
  • Example 9 Creation of an LHA-EGF chimaeric protein that incorporates a Thrombin recognition site into the LC
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/A and the EGF sequence (SEQ ID 8) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Thrombin (GRG).
  • Simple text character analysis of the primary sequence identified the sequence 103 GRM 105 within the LC domain.
  • the location of the peptide in the tertiary structure of the LC/A is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 7 (encoding the ORF of SEQ ID 8) using a primer designed to switch the codon for Met105 (ATG) to one that encodes Gly (GGT) was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Thrombin site is illustrated as SEQ ID 9 and the amino acid sequence of the expression product is illustrated in SEQ ID 10.
  • Example 10 Creation of an LHC-EGF chimaeric protein that incorporates a furin recognition site into the LC
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/C and the human epidermal growth factor sequence (SEQ ID 2) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for furin (RXR ⁇ K/R).
  • Simple text character analysis of the primary sequence identified the sequence 210 GEGR 213 within the LC domain.
  • the location of the peptide in the tertiary structure of the LC/C is predicted from examination of the location of the homologous peptide sequence in the LC/A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • SEQ ID 1 Site directed mutagenesis of the SEQ ID 1 (encoding SEQ ID 2) using a primer designed to switch the peptide region from GEGR to RSRR was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the furin site is illustrated as SEQ ID 11 and the amino acid sequence of the expression product is illustrated in SEQ ID 12.
  • Example 11 Creation of an LHA-EGF chimaeric protein that incorporates a Factor Xa recognition site into the H N domain
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/A and the human epidermal growth factor sequence (SEQ ID 8) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Factor Xa (IEGR).
  • Simple text character analysis of the primary sequence identified the sequence 562 GKSR 565 within the H N domain.
  • the location of the peptide in the tertiary structure of the H N /A is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 7 (encoding SEQ ID 8) using a primer designed to switch the peptide region from GKSR to IEGR was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Factor Xa site is illustrated as SEQ ID 13 and the amino acid sequence of the expression product is illustrated in SEQ ID 14.
  • Example 12 Creation of a LHA-EGF chimaeric protein that incorporates an ADAM17 recognition site into the LC domain
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/A and the human epidermal growth factor sequence (SEQ ID 8) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for ADAM17 (PLAQAVRSSS).
  • Simple text character analysis of the primary sequence identifies a region of the structure ( 206 PLLGAGKFAT 215 within the LC domain) that is amenable to protein engineering.
  • the location of the peptide in the tertiary structure of the LC is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 7 (which encodes SEQ ID 8) was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • the mutagenesis of the LC was performed to modify the coding region from 206 PLLGAGKFAT 215 to PLAQAVRSSS.
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the additional ADAM17 sites is illustrated as SEQ ID 15 and the amino acid sequence of the expression product is illustrated in SEQ ID 16.
  • the primary sequence of a recombinant endopeptidase active BoNT/A containing an engineered activation protease site specific for enterokinase is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for ADAM17 (PLAQAVRSSS).
  • Simple text character analysis of the primary sequence identifies a region of the BoNT structure ( 206 PLLGAGKFAT 215 within the LC domain) that is amenable to protein engineering.
  • the location of the peptide in the tertiary structure of the LC is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 17 (which encodes SEQ ID 18) was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • the mutagenesis of the LC was performed to modify the coding region from 206 PLLGAGKFAT 215 to PLAQAVRSSS.
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the additional ADAM17 sites is illustrated as SEQ ID 19 and the amino acid sequence of the expression product is illustrated in SEQ ID 20.
  • the primary sequence of a recombinant endopeptidase active BoNT/A containing an engineered activation protease site specific for enterokinase (SEQ ID 18) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for furin (RXR ⁇ K/R).
  • Simple text character analysis of the primary sequence identified the sequence 563 KSR 565 within the H N domain that is amenable to protein engineering..
  • the location of the peptide in the tertiary structure of the H N domain is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 17 (which encodes SEQ ID 18) using a primer designed to switch the codon for K 563 (AAA) to Arg (CGT) and to insert an Arg (CGC) after the existing R 565 changes the sequence 563 KSR 565 to RSRR which is a substrate for cleavage by furin. Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the additional ADAM17 sites is illustrated as SEQ ID 21 and the amino acid sequence of the expression product is illustrated in SEQ ID 22.
  • Example 15 Treatment of a patient suffering from dystonia (Spasmodic Torticollis)
  • the patient is subsequently treated by injection with up to about 300 units, or more, of polypeptide of the present invention (eg.
  • the physician is able to inject more product into the area requiring therapy without fear of an increase in side effects. Enhanced dose leads to enhanced duration of action and therefore improved therapy.
  • Example 16 Treatment of a patient suffering from blepharospasm
  • a 58 year old female with blepharospasm is treated by injecting between about 1 to about 5 units of a polypeptide of the present invention (eg. a botulinum toxin type A neurotoxin modified to include a ADAM17 protease sensitive site, as described in Example 13) into the lateral pre-tarsal orbicularis oculi muscle of the upper lid and the lateral pre-tarsal orbicularis oculi of the lower lid, the amount injected varying based upon both the size of the muscle to be injected and the extent of muscle paralysis desired. Alleviation of the blepharospasm occurs in about 1 to about 7 days.
  • the physician is able to inject more product into the area requiring therapy without fear of an increase in side effects. Enhanced dose leads to enhanced duration of action and therefore improved therapy.
  • Example 17 Creation of a LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the LC at position 210 [SXN101975]
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/C and the human epidermal growth factor sequence (SEQ ID 2) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Factor Xa (IEGR).
  • IEGR prototypical recognition site for Factor Xa
  • a site for insertion of a Factor Xa site is identified in the primary sequence 210 GEGR within the LC domain.
  • the location of the peptide in the tertiary structure of the LC/C is predicted from examination of the location of the homologous peptide sequence in the LC/A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 1 was achieved using a primer designed to switch the codons for 210 G to I to make it a substrate for Factor Xa cleavage. Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology). E . coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • Geneart Graphical Codon Usage Analyser
  • %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Factor Xa site is illustrated as SEQ ID 23 and the amino acid sequence of the expression product is illustrated in SEQ ID 24.
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/C and the human epidermal growth factor sequence (SEQ ID 2) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Thrombin.
  • a site for insertion of a Thrombin site is identified in the primary sequence 194 ISPRFM 199 within the LC domain.
  • the location of the peptide in the tertiary structure of the LC/C is predicted from examination of the location of the homologous peptide sequence in the LC/A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 1 (encoding SEQ ID 2) using a primer designed to switch the codons for S 195 to Val (TCT to GTT) and M 195 to Ser (ATG to TCC) changes the region 194 ISPRFM 199 to IVPRFS to make it a substrate for Thrombin cleavage. Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology). E .
  • coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Thrombin site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 25.
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/C and the human epidermal growth factor sequence (SEQ ID 2) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Thrombin.
  • a site for insertion of a Thrombin site is identified in the primary sequence 210 GEGRFS within the LC domain.
  • the location of the peptide in the tertiary structure of the LC/C is predicted from examination of the location of the homologous peptide sequence in the LC/A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 1 (encoding SEQ ID 2) using a primer designed to switch the codons 211 EGR to TPR to create a sequence GTPRFS which is a substrate for Thrombin cleavage.
  • Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Thrombin site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 26.
  • Example 20 Creation of a LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the H N domain at position 742 of the H N [SXN101937]
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/C and the human epidermal growth factor sequence (SEQ ID 2) is reviewed and a site for insertion of a Factor Xa site is identified in the primary sequence 742 IDLE 755 within the H N domain.
  • the location of the peptide in the tertiary structure of the H N /C is predicted from examination of the location of the homologous peptide sequence in the H N /A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 1 (encoding SEQ ID 2) using a primer designed to switch the codons for 742 LE to GR to make it a substrate for Factor Xa cleavage.
  • Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Thrombin site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 27.
  • Example 21 Creation of a LHC-EGF chimaeric protein that incorporates a Factor Xa recognition site into the H N domain at position 750 of the H N [SXN101938]
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/C and the human epidermal growth factor sequence (SEQ ID 2) is reviewed and a site for insertion of a Factor Xa site is identified in the primary sequence 750 SGSD 753 within the H N domain.
  • the location of the peptide in the tertiary structure of the H N /C is predicted from examination of the location of the homologous peptide sequence in the H N /A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 1 (encoding SEQ ID 2) using a primer designed to switch the codons for 750 SGSD to IDGR make it a substrate for Factor Xa cleavage.
  • Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E . coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Factor Xa site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 28.
  • Example 22 Creation of a LHC-EGF chimaeric protein that incorporates a Thrombin recognition site into the H N domain at position 750 of the H N [SXN101939]
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/C and the human epidermal growth factor sequence (SEQ ID 2) is reviewed and a site for insertion of a Thrombin site is identified in the primary sequence 750 SGSD 753 within the H N domain.
  • the location of the peptide in the tertiary structure of the H N /C is predicted from examination of the location of the homologous peptide sequence in the H N /A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 1 (encoding SEQ ID 2) using a primer designed to switch the codons for SGSD to GVPR to make it a substrate for Thrombin cleavage.
  • Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Thrombin site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 29.
  • Example 23 Creation of a LHD-VIPr chimaeric protein that incorporates a Factor Xa recognition site into the H N domain at position 798 of the H N [SXN101930]
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/D and an analogue of the human vasoactive intestinal peptide (VIPr) is reviewed and a site for insertion of a Factor Xa site is identified in the primary sequence 798 SGSD within the H N domain.
  • the location of the peptide in the tertiary structure of the H N /D is predicted from examination of the location of the homologous peptide sequence in the H N /A using the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Factor Xa site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 30.
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/A and the human epidermal growth factor sequence (SEQ ID 8) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Thrombin (GRG).
  • Simple text character analysis of the primary sequence identified the sequence 103 GRM 105 within the LC domain.
  • the location of the peptide in the tertiary structure of the LC is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • SEQ ID 7 Site directed mutagenesis of the SEQ ID 7 (encoding SEQ ID 8) using a primer designed to switch the peptide region from GRM to GRG was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Thrombin site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 31.
  • Example 25 Demonstration of specific cleavage of a purified LHC-EGF chimaeric protein that is engineered to incorporate a Factor Xa recognition site into the LC [SXN1975]
  • Example 17 A novel molecule incorporating a Factor Xa recognition site into the LC of LHC-EGF is constructed according to Example 17. Using methodology similar to that described in Example 6, the protein of Example 17 is expressed and purified. The methodology was adapted for use on an AKTA Xpress purification system. Essentially, the clarified E.coli lysates were applied to a 5ml HisTrap FF Crude column on the Xpress system. The program was set to wash the columns with 10 column volumes of binding buffer (50mM Tris pH8.0, 200mM NaCl) and 10 col. vols. of 40mM imidazole in binding buffer (collected together with the flow through). Elution was with 5 col. vols. of 250mM imidazole in binding buffer.
  • binding buffer 50mM Tris pH8.0, 200mM NaCl
  • Figure 4 illustrates the cleavage of the protein in the presence of Factor Xa. Cleavage products are observed in the non-reduced and reduced samples. The estimated mass of the cleavage products is in agreement with the anticipated cleavage point of the engineered protein
  • Example 26 Demonstration of specific cleavage of a purified LHC-EGF chimaeric protein that is engineered to incorporate a Factor Xa recognition site into the H N [SXN1937 & SXN1938]
  • a novel molecule incorporating a Factor Xa recognition site into the H N of LHC-EGF is constructed according to Example 20, and a second novel jmolecule incorporating a Factor Xa recognition site into a different location within the H N of LHC-EGF is constructed according to Example 21.
  • the proteins of Example 20 and 21 are expressed and purified.
  • Figure 5 illustrates purification of LHC-EGF from Example 20 from E. coli
  • Figure 6 illustrates purification of LHC-EGF from Example 21 from E . coli.
  • Example 7 illustrates the cleavage of the protein in the presence of Factor Xa, as assessed by staining of SDS-PAGE gels.
  • Figure 8 illustrates the profile of the samples when assessed by Western blotting using anti-His tag antibodies to probe for the presence of the His tag. The estimated mass of the cleavage products is in agreement with the anticipated cleavage point of the engineered protein.
  • Example 21 Using methodology described in Example 7, the protein of Example 21 is treated with Factor Xa protease and samples analysed by SDS-PAGE.
  • Figure 9 illustrates the cleavage of the protein in the presence of Factor Xa. The estimated mass of the cleavage products is in agreement with the anticipated cleavage point of the engineered protein.
  • Example 27 Demonstration of specific cleavage of a purified LHC-EGF chimaeric protein that is engineered to incorporate a Thrombin recognition site into the LC [SXN1932]
  • a novel molecule incorporating a Thrombin recognition site into the LC of LHC-EGF is constructed according to Example 19. Using methodology similar to that described in Example 25, the protein of Example 19 is expressed and purified. Figure 10 illustrates purification of LHC-EGF from E. coli.
  • the protein is treated with Thrombin protease and samples analysed by SDS-PAGE.
  • Figure 11 illustrates the cleavage of the protein in the presence of Thrombin, as assessed by SDS-PAGE.
  • Figure 12 illustrates the cleavage of the protein in the presence of Thrombin, as assessed by Western blotting using anti-EGF antibodies. The estimated mass of the cleavage products is in agreement with the anticipated cleavage point of the engineered protein
  • Example 28 Demonstration of specific cleavage of a purified LHA-EGF chimaeric protein that is engineered to incorporate a Thrombin recognition site into the LC [SXN1974]
  • a novel molecule incorporating a Factor Xa recognition site into the LC of LHA-EGF is constructed according to Example 24. Using methodology similar to that described in Example 25, the protein of Example 24 is expressed and purified. Figure 13 illustrates purification of LHA-EGF from E. coli.
  • Figure 14 illustrates the cleavage of the protein in the presence of Thrombin.
  • Figure 15 illustrates the Western blot profile of the same PAGE, using anti-EGF as primary antibody. The estimated mass of the cleavage products is in agreement with the anticipated cleavage point of the engineered protein
  • Example 29 Demonstration of reduced in vitro cellular activity of a protein engineered to incorporate a FXa protease cleavage site into the LC domain of LHC-EGF [SXN1975]
  • the protein product of Example 25 is expressed and purified.
  • the purified protein is exposed to FXa protease for prior to assessment in an in vitro spinal cord neuron (SCN) assay.
  • SCN spinal cord neuron
  • the preparation of SCN is a well established technique and is described in the literature [ B.R. Ransom, E. Neale, M. Henkart, P.N. Bullock, P.G. Nelson, Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties, J. Neurophysiol. 40 (1977) 1132-1150 ; S.C. Fitzgerald, A Dissection and Tissue Culture Manual of the Nervous System, Alan R. Liss Inc, New York, 1989 ].
  • Test protein is prepared at a variety of concentrations by dilution into culture media.
  • SCNs are exposed to the test proteins for 24hr prior to removal of media and preparation of the cellular material for analysis by SDS-PAGE and Western blotting. Following separation of cellular proteins on Novex 4-20% Tris-glycine polyacrylamide gels, the proteins are transferred to nitrocellulose and subsequently probed for the presence of the appropriate SNARE protein using antibodies obtained from commercial sources. In this case, the antibodies were specific for the SNARE syntaxin.
  • the protein that has been treated with Factor Xa is clearly less effective at cleaving Syntaxin than the protein that was not treated with FXa.
  • the invention has therefore enabled a reduction in the efficacy of the modified protein.
  • Example 30 Demonstration of reduced in vitro cellular activity of a protein engineered to incorporate a Thrombin protease cleavage site into the LC domain of LHA-EGF [SXN1974]
  • the protein product of Example 24 is expressed and purified.
  • the purified protein is exposed to Thrombin protease for prior to assessment in an in vitro spinal cord neuron (SCN) assay.
  • SCN spinal cord neuron
  • the preparation of SCN is a well established technique and is described in the literature [ B.R. Ransom, E. Neale, M. Henkart, P.N. Bullock, P.G. Nelson, Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties, J. Neurophysiol. 40 (1977) 1132-1150 ; S.C. Fitzgerald, A Dissection and Tissue Culture Manual of the Nervous System, Alan R. Liss Inc, New York, 1989 ].
  • Test protein is prepared at a variety of concentrations by dilution into culture media.
  • SCNs are exposed to the test proteins for 24hr prior to removal of media and preparation of the cellular material for analysis by SDS-PAGE and Western blotting. Following separation of cellular proteins on Novex 4-20% Tris-glycine polyacrylamide gels, the proteins are transferred to nitrocellulose and subsequently probed for the presence of the appropriate SNARE protein using antibodies obtained from commercial sources. In this case, the antibodies were specific for the SNARE SNAP-25.
  • Figure 17 demonstrates SNAP-25-cleavage by thrombin-treated L(Thr)HA-EGF compared to untreated L(Thr)HA-EGF.
  • the primary sequence of a recombinant endopeptidase active BoNT/A containing an engineered activation protease site specific for enterokinase is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for thrombin (GRG).
  • Simple text character analysis of the primary sequence identified the sequence 103 GRM 105 within the LC domain that is amenable to protein engineering.
  • the location of the peptide in the tertiary structure of the H N domain is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 17 (which encodes SEQ ID 18) using a primer designed to switch the codons for M 105 to G changes the sequence 103 GRM 105 to GRG which is a substrate for cleavage by thrombin. Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final amino acid sequence of the expression product is illustrated in SEQ ID 32.
  • the primary sequence of a recombinant endopeptidase active BoNT/A containing an engineered activation protease site specific for enterflkinase (SEQ ID 18) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Factor Xa (IEGR).
  • Simple text character analysis of the primary sequence identified the sequence IDSL within the LC domain that is amenable to protein engineering.
  • the location of the peptide in the tertiary structure of the LC domain is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis of the SEQ ID 17 (which encodes SEQ ID 18) using a primer designed to switch the codons for 276 SL to GR changes the sequence IDSL to IDGR which is a substrate for cleavage by Factor Xa. Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E . coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final amino acid sequence of the expression product is illustrated in SEQ ID 33.
  • the primary sequence of a recombinant endopeptidase active BoNT/A containing an engineered activation protease site specific for enterokinase (SEQ ID 18) is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Factor Xa (IEGR).
  • Simple text character analysis of the primary sequence identified the sequence 562 GKSR 565 within the H N domain that is amenable to protein engineering.
  • the location of the peptide in the tertiary structure of the H N domain is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • E . coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final amino acid sequence of the expression product is illustrated in SEQ ID 34.
  • BoNT/E The primary sequence of a recombinant endopeptidase active BoNT/E [nucleotide accession AM695755; Uniprot number A8Y867] is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Thrombin (LVPRGS).
  • Simple text character analysis of the primary sequence identified the sequence 186 FSPEYS 191 within the LC domain that is amenable to protein engineering.
  • the location of the peptide in the tertiary structure of the H N domain is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis is achieved using a primer designed to switch the peptide region from FSPEYS to IVPRFS which is a substrate for cleavage by Thrombin. Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E . coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final amino acid sequence of the expression product is illustrated in SEQ ID 35.
  • BoNT/E The primary sequence of BoNT/E [nucleotide accession AM695755; Uniprot number A8Y867] is reviewed for a potential insertion site for a Factor Xa recognition peptide (IEGR). Comparison of the primary sequence of BoNT/E with that of BoNT/A and the corresponding location of the peptide in the tertiary structure of the H N domain predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA), concludes that the region 727 TLEE is suitable for protein engineering to IEGR.
  • IEGR Factor Xa recognition peptide
  • Site directed mutagenesis is achieved using a primer designed to switch the peptide region from TLEE to IEGR which is a substrate for cleavage by Factor Xa. Mutagenesis was achieved utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E . coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final amino acid sequence of the expression product is illustrated in SEQ ID 36.
  • Example 36 Creation of an LHE-VIPr chimaeric protein that incorporates a Thrombin recognition site into the LC
  • the primary sequence of a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/E and an analogue of the human vasoactive intestinal peptide (VIPr) sequence is reviewed for the presence of amino acid strings that bear resemblance to the prototypical recognition site for Thrombin (GRG).
  • Simple text character analysis of the primary sequence identified the sequence 103 GGI 105 within the LC domain of the chimaera.
  • the location of the peptide in the tertiary structure of the LC/E is predicted from the X-ray crystal structure of LC/E (pdb: 1T3A) as the guide.
  • Site directed mutagenesis is achieved using a primer designed to switch the peptide region from GGI to GRG utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Thrombin site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 37.
  • Example 37 Creation of an LHE-VIPr chimaeric protein that incorporates a Factor Xa recognition site into the H N
  • VIPr vasoactive intestinal peptide
  • Simple text character analysis of the primary sequence identified the sequence 585 GENN within the H N domain.
  • the location of the peptide in the tertiary structure of the H N /E is predicted from the X-ray crystal structure of BoNT/A (pdb: 3BTA) as the guide.
  • Site directed mutagenesis is achieved using a primer designed to switch the peptide region from GENN to IEGR utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Factor Xa site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 38.
  • Example 38 Creation of an LHE-VIPr chimaeric protein that incorporates a Factor Xa recognition site into the LC
  • a chimaeric protein constructed by a genetic fusion of the LH N fragment of BoNT/E (incorporating a mutated substrate recognition domain (K228D) and an analogues of the human vasoactive intestinal peptide (VIPr) is reviewed for the presence of amino acid strings that are exposed on the surface of the protein and can be engineered to resemble the prototypical recognition site for Factor Xa (IEGR).
  • IEGR IEGR
  • the location of the peptide in the tertiary structure of the LC/E is predicted from the X-ray crystal structure of BoNT/E (pdb: 1T3A) as the guide.
  • Site directed mutagenesis is achieved using a primer designed to switch the peptide region from VAQY to IEGR utilising standard molecular tools for performing mutagenesis (for example, the Stratagene Quickchange mutagenesis methodology).
  • E. coli codon usage is assessed by reference to software programs such as Graphical Codon Usage Analyser (Geneart), and the %GC content and codon usage ratio assessed by reference to published codon usage tables (for example GenBank Release 143, September 13 2004) to ensure that the mutagenesis does not result in poor codon utilisation.
  • the mutagenised DNA is incorporated into a standard cloning vector, for example pCR4, prior to transformation into E. coli host.
  • the integrity of the ORF DNA is checked by sequencing.
  • the final ORF incorporating the Factor Xa site is used to encode the amino acid sequence of the expression product is illustrated in SEQ ID 39.
  • Example 39 Cleavage of SNARE protein by a modified clostridial neurotoxin (LH N ) having the properties described by LIN, et al. (WO02/044199)
  • Embryonic spinal cord neurons were prepared by dissection from E15 Sprague Dawley rats and dissociated before plating onto Matrigel-coated 96 well plates at 125,000 cells per well in medium (MEM buffered with sodium bicarbonate, 5% inactivated horse serum, 0.6% D-glucose, 2% N1 medium supplement, 40ng/ml corticosterone, 20ng/ml tri-iodothryronine).
  • the cells were incubated with fresh medium containing either recombinant light chain of serotype C (LC/C) or a modified clostridial neurotoxin consisting of the translocation and light chains of serotype C (LHn/C) at half log concentrations between 180 nM and 0.18 nM) for 24hrs at 37°C in a humidified, 5% CO 2 atmosphere.
  • LC/C recombinant light chain of serotype C
  • LHn/C modified clostridial neurotoxin consisting of the translocation and light chains of serotype C
  • the Figure 1 shows cleaved syntaxin as a percentage of total syntaxin, and confirms a neurotoxin activity for the modified clostridial neurotoxin lacking a functional H C binding domain (LHn/C), but no detectable neurotoxin activity for the modified clostridial neurotoxin lacking a functional H N translocation domain (LC/C).

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CN114957482B (zh) * 2021-02-26 2024-09-24 重庆誉颜制药有限公司 一种经修饰的神经毒素的单链多肽及其用途
GB202103372D0 (en) 2021-03-11 2021-04-28 Ipsen Biopharm Ltd Modified clostridial neurotoxins
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