EP2366773A3 - PUFA polyketide synthase systems and uses thereof - Google Patents

PUFA polyketide synthase systems and uses thereof Download PDF

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Publication number
EP2366773A3
EP2366773A3 EP10178962A EP10178962A EP2366773A3 EP 2366773 A3 EP2366773 A3 EP 2366773A3 EP 10178962 A EP10178962 A EP 10178962A EP 10178962 A EP10178962 A EP 10178962A EP 2366773 A3 EP2366773 A3 EP 2366773A3
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EP
European Patent Office
Prior art keywords
polyketide synthase
pufa
bacterial
nucleic acid
acid molecules
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EP10178962A
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German (de)
French (fr)
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EP2366773A2 (en
EP2366773B1 (en
Inventor
James G. Metz
William R. Barclay
James H. Flatt
Jerry M. Kuner
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DSM IP Assets BV
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Martek Biosciences Corp
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    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
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    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKA) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to methods of making and using such system for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
EP10178962.6A 2001-04-16 2002-04-16 Pufa polyketide synthase systems and uses thereof Expired - Lifetime EP2366773B1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US28406601P 2001-04-16 2001-04-16
US29879601P 2001-06-15 2001-06-15
US32326901P 2001-09-18 2001-09-18
EP02731415.2A EP1385934B1 (en) 2001-04-16 2002-04-16 Pufa polyketide synthase systems and uses thereof

Related Parent Applications (3)

Application Number Title Priority Date Filing Date
EP02731415.2A Division-Into EP1385934B1 (en) 2001-04-16 2002-04-16 Pufa polyketide synthase systems and uses thereof
EP02731415.2A Division EP1385934B1 (en) 2001-04-16 2002-04-16 Pufa polyketide synthase systems and uses thereof
EP02731415.2 Division 2002-04-16

Publications (3)

Publication Number Publication Date
EP2366773A2 EP2366773A2 (en) 2011-09-21
EP2366773A3 true EP2366773A3 (en) 2012-09-05
EP2366773B1 EP2366773B1 (en) 2017-03-22

Family

ID=27403446

Family Applications (5)

Application Number Title Priority Date Filing Date
EP10178950.1A Expired - Lifetime EP2366772B1 (en) 2001-04-16 2002-04-16 PUFA polyketide synthase systems and uses thereof
EP10178976A Withdrawn EP2366774A3 (en) 2001-04-16 2002-04-16 PUFA polyketide synthase systems and uses thereof
EP02731415.2A Expired - Lifetime EP1385934B1 (en) 2001-04-16 2002-04-16 Pufa polyketide synthase systems and uses thereof
EP10178938A Withdrawn EP2366771A3 (en) 2001-04-16 2002-04-16 PUFA polyketide synthase systems and uses thereof
EP10178962.6A Expired - Lifetime EP2366773B1 (en) 2001-04-16 2002-04-16 Pufa polyketide synthase systems and uses thereof

Family Applications Before (4)

Application Number Title Priority Date Filing Date
EP10178950.1A Expired - Lifetime EP2366772B1 (en) 2001-04-16 2002-04-16 PUFA polyketide synthase systems and uses thereof
EP10178976A Withdrawn EP2366774A3 (en) 2001-04-16 2002-04-16 PUFA polyketide synthase systems and uses thereof
EP02731415.2A Expired - Lifetime EP1385934B1 (en) 2001-04-16 2002-04-16 Pufa polyketide synthase systems and uses thereof
EP10178938A Withdrawn EP2366771A3 (en) 2001-04-16 2002-04-16 PUFA polyketide synthase systems and uses thereof

Country Status (9)

Country Link
EP (5) EP2366772B1 (en)
JP (4) JP2005510203A (en)
CN (2) CN1535312A (en)
AR (1) AR040622A1 (en)
AU (2) AU2002303394B2 (en)
CA (1) CA2444164C (en)
ES (3) ES2562766T3 (en)
TW (3) TWI337619B (en)
WO (1) WO2002083870A2 (en)

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US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US6566583B1 (en) 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US7247461B2 (en) 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7217856B2 (en) 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7211418B2 (en) 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
ES2208141T5 (en) 2000-01-28 2015-11-26 Dsm Ip Assets B.V. Increased production of lipids containing polyenoic fatty acids by high-density cultures of eukaryotic microbes in fermenters
CA2509227C (en) * 2002-12-19 2013-05-21 Monsanto Technology, Llc Elevation of oil levels in brassica plants
AU2004225485B2 (en) * 2003-03-26 2008-08-21 Dsm Ip Assets B.V. PUFA polyketide synthase systems and uses thereof
US7700320B2 (en) * 2004-02-13 2010-04-20 Martek Biosciences Corporation Schizochytrium fatty acid synthase (FAS) and products and methods related thereto
DE102004017369A1 (en) * 2004-04-08 2005-11-03 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Screening method for the identification of PUFA-PKS in samples
DE102004017370A1 (en) * 2004-04-08 2005-10-27 Nutrinova Nutrition Specialties & Food Ingredients Gmbh PUFA-PKS Genes from Ulkenia
CA2884237C (en) 2004-04-22 2020-09-08 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
US7807849B2 (en) 2004-04-22 2010-10-05 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
WO2006135866A2 (en) * 2005-06-10 2006-12-21 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof
JP4819888B2 (en) 2005-07-01 2011-11-24 マーテック バイオサイエンシーズ コーポレーション Polyunsaturated fatty acid-containing oil products and their use and production
EP2001277B9 (en) 2006-03-15 2014-11-26 DSM IP Assets B.V. Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
BRPI0716075A2 (en) 2006-08-29 2013-08-06 Commw Scient Ind Res Org Fatty Acid Synthesis
MX2009002285A (en) * 2006-08-29 2011-10-11 Market Biosciences Corp USE OF DPA(n-6) OILS IN INFANT FORMULA.
EP3260544A1 (en) 2008-11-18 2017-12-27 Commonwealth Scientific and Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
EP2408797B1 (en) * 2009-03-19 2017-03-15 DSM IP Assets B.V. Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof
CA2876519C (en) 2012-06-15 2020-09-15 Commonwealth Scientific And Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
SG11201604871VA (en) 2013-12-18 2016-07-28 Commw Scient Ind Res Org Lipid comprising long chain polyunsaturated fatty acids
CA2953008C (en) 2014-06-27 2024-03-19 Nuseed Pty Ltd Lipid comprising docosapentaenoic acid
US20210309987A1 (en) * 2018-08-10 2021-10-07 Kyowa Hakko Bio Co., Ltd. Microorganism producing polyunsaturated fatty acid and method for producing polyunsaturated fatty acid
JPWO2020032261A1 (en) * 2018-08-10 2021-08-10 協和発酵バイオ株式会社 Microorganisms that produce eicosapentaenoic acid and methods for producing eicosapentaenoic acid
CN114555401A (en) * 2019-10-23 2022-05-27 索尼集团公司 Display system, display device, display method, and mobile device

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US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
WO1998055625A1 (en) * 1997-06-04 1998-12-10 Calgene, Llc Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
WO2000042195A2 (en) * 1999-01-14 2000-07-20 Omegatech, Inc. Schizochytrium pks genes

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