EP1047710B1 - Orotidine 5'-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof - Google Patents

Orotidine 5'-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof

Info

Publication number
EP1047710B1
EP1047710B1 EP98966388A EP98966388A EP1047710B1 EP 1047710 B1 EP1047710 B1 EP 1047710B1 EP 98966388 A EP98966388 A EP 98966388A EP 98966388 A EP98966388 A EP 98966388A EP 1047710 B1 EP1047710 B1 EP 1047710B1
Authority
EP
European Patent Office
Prior art keywords
gene
orotidine
utilization
phosphate decarboxylase
construct containing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP98966388A
Other languages
German (de)
French (fr)
Other versions
EP1047710A2 (en
Inventor
Markus Pompejus
Jose Luis Revuelta Doval
Maria Angeles Santos Garcia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
BASF SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE filed Critical BASF SE
Publication of EP1047710A2 publication Critical patent/EP1047710A2/en
Application granted granted Critical
Publication of EP1047710B1 publication Critical patent/EP1047710B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01023—Orotidine-5'-phosphate decarboxylase (4.1.1.23)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to an orotidine-5'-phosphate decarboxylase gene having the sequence of SEQ ID NO: 1 or its homologs, to a gene construct comprising this gene or its homologs, and to its use. The invention additionally relates to vectors or organisms comprising an orotidine-5'-phosphate decarboxylases gene having the sequence SEQ ID NO: 1 or its homologs. The invention further relates to a process for producing uracil-auxotrophic microorganisms and to a process for inserting DNA into uracil-auxotrophic microorganisms.
EP98966388A 1998-01-15 1998-12-18 Orotidine 5'-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof Expired - Lifetime EP1047710B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19801120A DE19801120A1 (en) 1998-01-15 1998-01-15 New orotidine-5'-phosphate decarboxylase gene useful as selectable marker for microbial transformation
DE19801120 1998-01-15
PCT/EP1998/008382 WO1999036432A2 (en) 1998-01-15 1998-12-18 Orotidine 5'-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof

Publications (2)

Publication Number Publication Date
EP1047710A2 EP1047710A2 (en) 2000-11-02
EP1047710B1 true EP1047710B1 (en) 2005-06-01

Family

ID=7854573

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98966388A Expired - Lifetime EP1047710B1 (en) 1998-01-15 1998-12-18 Orotidine 5'-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof

Country Status (9)

Country Link
US (1) US6927026B1 (en)
EP (1) EP1047710B1 (en)
JP (1) JP2002508950A (en)
CN (1) CN1161471C (en)
AT (1) ATE296835T1 (en)
DE (2) DE19801120A1 (en)
IL (1) IL137006A0 (en)
WO (1) WO1999036432A2 (en)
ZA (1) ZA99236B (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1629100B1 (en) * 2003-06-02 2010-09-15 B.R.A.I.N. Biotechnology Research And Information Network AG Archaeon expression system
CN1906294B (en) * 2003-11-19 2013-09-11 陶氏环球技术公司 Improved protein expression systems
US8067391B2 (en) * 2005-10-03 2011-11-29 University Health Network ODCase inhibitors for the treatment of malaria
GB2433260A (en) * 2005-12-16 2007-06-20 Mologic Ltd A selectable decarboxylase marker
JP6092501B2 (en) * 2011-06-02 2017-03-08 三井造船株式会社 Uracil-requiring Morella bacteria and transforming transgenic Morella bacteria
EP2935570B1 (en) 2012-12-20 2018-09-19 DSM IP Assets B.V. Carotene hydroxylase and its use for producing carotenoids
EP2935571B1 (en) 2012-12-20 2018-03-07 DSM IP Assets B.V. Acetyl transferases and their use for producing carotenoids
CN112391329B (en) * 2020-11-12 2023-07-25 江南大学 Escherichia coli engineering bacteria with improved acid stress resistance and application thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2441659A1 (en) * 1978-11-14 1980-06-13 Anvar NOVEL HYBRID PLASMIDS AND MICROORGANISMS CONTAINING THEM
DE4020181A1 (en) 1990-06-25 1992-01-02 Basf Ag NEW PROMOTERREGION
DE4420785A1 (en) * 1994-03-25 1995-10-05 Basf Ag Riboflavin biosynthesis in fungi
DK0751995T3 (en) 1994-03-25 2004-03-08 Basf Ag Riboflavin biosynthesis in fungi
DK0839211T3 (en) * 1995-07-13 2001-09-03 Basf Ag Process for the preparation of riboflavin by microorganisms with altered isocitrate lyase activity

Also Published As

Publication number Publication date
WO1999036432A2 (en) 1999-07-22
IL137006A0 (en) 2001-06-14
WO1999036432A3 (en) 1999-08-19
CN1161471C (en) 2004-08-11
ATE296835T1 (en) 2005-06-15
DE19801120A1 (en) 1999-07-22
ZA99236B (en) 2000-07-14
EP1047710A2 (en) 2000-11-02
JP2002508950A (en) 2002-03-26
CN1285873A (en) 2001-02-28
DE59812837D1 (en) 2005-07-07
US6927026B1 (en) 2005-08-09

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