EP2357228A4 - Novel fructosyl peptide oxidase - Google Patents

Novel fructosyl peptide oxidase

Info

Publication number
EP2357228A4
EP2357228A4 EP09819249A EP09819249A EP2357228A4 EP 2357228 A4 EP2357228 A4 EP 2357228A4 EP 09819249 A EP09819249 A EP 09819249A EP 09819249 A EP09819249 A EP 09819249A EP 2357228 A4 EP2357228 A4 EP 2357228A4
Authority
EP
European Patent Office
Prior art keywords
fructosyl peptide
peptide oxidase
novel fructosyl
novel
oxidase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP09819249A
Other languages
German (de)
French (fr)
Other versions
EP2357228B1 (en
EP2357228A1 (en
Inventor
Kazuo Aisaka
Keiko Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Chemical Diagnostics Systems Co Ltd
Original Assignee
Kyowa Medex Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Medex Co Ltd filed Critical Kyowa Medex Co Ltd
Publication of EP2357228A1 publication Critical patent/EP2357228A1/en
Publication of EP2357228A4 publication Critical patent/EP2357228A4/en
Application granted granted Critical
Publication of EP2357228B1 publication Critical patent/EP2357228B1/en
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/906—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
    • G01N2333/9065—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on CH-NH groups of donors (1.5)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/042—Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
EP09819249.5A 2008-10-09 2009-10-08 Novel fructosyl peptide oxidase Not-in-force EP2357228B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008262601 2008-10-09
PCT/JP2009/067565 WO2010041715A1 (en) 2008-10-09 2009-10-08 Novel fructosyl peptide oxidase

Publications (3)

Publication Number Publication Date
EP2357228A1 EP2357228A1 (en) 2011-08-17
EP2357228A4 true EP2357228A4 (en) 2012-04-25
EP2357228B1 EP2357228B1 (en) 2017-05-10

Family

ID=42100663

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09819249.5A Not-in-force EP2357228B1 (en) 2008-10-09 2009-10-08 Novel fructosyl peptide oxidase

Country Status (8)

Country Link
US (3) US8304249B2 (en)
EP (1) EP2357228B1 (en)
JP (2) JP5665081B2 (en)
KR (1) KR20110069138A (en)
CN (1) CN102232111B (en)
CA (1) CA2739881A1 (en)
HK (1) HK1163734A1 (en)
WO (1) WO2010041715A1 (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8993255B2 (en) 2008-10-10 2015-03-31 Toyo Boseki Kabushiki Kaisha Protein having fructosyl valyl histidine oxidase activity, modified protein, and use of the protein or the modified protein
WO2012018094A1 (en) * 2010-08-06 2012-02-09 キッコーマン株式会社 Amadoriase having altered substrate specificity
US9701949B2 (en) 2011-12-28 2017-07-11 Kikkoman Corporation Amadoriase with improved thermostability, gene and recombinant DNA for the amadoriase, and method for production of amadoriase with improved thermostability
US9994827B2 (en) 2012-01-13 2018-06-12 Toyobo Co., Ltd. Method for producing fructosyl valyl histidine oxidase preparation
KR101207418B1 (en) * 2012-02-10 2012-12-04 주식회사 아이센스 Hemolysis reagent composition for quantitative analysis of glycated hemoglobin using enzyme method
WO2013162035A1 (en) 2012-04-27 2013-10-31 キッコーマン株式会社 Modified amadoriase capable of acting on fructosyl hexapeptide
JP6363327B2 (en) * 2013-03-25 2018-07-25 キッコーマン株式会社 Modified amadoriase
JP6328115B2 (en) * 2013-07-09 2018-05-23 協和メデックス株式会社 Glycated hexapeptide oxidase and its use
CN105431545B (en) 2013-07-09 2021-06-18 日立化成诊断系统株式会社 Method for measuring glycated hemoglobin
CN111398605B (en) 2013-08-09 2024-04-05 龟甲万株式会社 Glycosylated hemoglobin measurement composition
US9650489B2 (en) 2013-09-27 2017-05-16 Toyo Seikan Group Holdings, Ltd. Method for degrading biodegradable resin
JP6233566B2 (en) * 2013-09-27 2017-11-22 東洋製罐グループホールディングス株式会社 Degradation method of biodegradable resin
CN105683390B (en) 2013-10-25 2021-02-26 龟甲万株式会社 HbA1c measurement method using amadoriase acting on glycosylated peptide
CN105683374A (en) 2013-10-25 2016-06-15 龟甲万株式会社 HEMOGLOBIN A1c MEASUREMENT METHOD AND MEASUREMENT KIT
BR112016019822B1 (en) * 2014-05-06 2023-11-14 Diasys Diagnostic Systems Gmbh METHOD FOR DETERMINING THE AMOUNT OF GLYCOSYLATED HEMOGLOBIN (HBA1C) IN A SAMPLE AND REAGENT KIT
WO2016063984A1 (en) * 2014-10-24 2016-04-28 キッコーマン株式会社 Amadoriase having enhanced dehydrogenase activity
JP7094655B2 (en) 2014-11-07 2022-07-04 キッコーマン株式会社 Amadriase with improved resistance to anionic surfactant
US10934530B2 (en) 2015-04-03 2021-03-02 Kikkoman Corporation Amadoriase having improved specific activity
WO2017183717A1 (en) 2016-04-22 2017-10-26 キッコーマン株式会社 HbA1c DEHYDROGENASE
TWI668229B (en) 2016-12-13 2019-08-11 財團法人工業技術研究院 Recombinant protein, and preparation method and application thereof
WO2018221446A1 (en) 2017-05-30 2018-12-06 協和メデックス株式会社 Method for measuring glycated hemoglobin
US20200283741A1 (en) 2017-08-31 2020-09-10 Kikkoman Corporation Glycated hemoglobin oxidase variant and method for measurement
JP6818718B2 (en) * 2018-06-28 2021-01-20 キッコーマン株式会社 Modified Amadriase
CN109593689B (en) * 2019-01-31 2022-09-02 贵州大学 Bacillus subtilis B-01 preparation for producing protease and nattokinase and application method thereof
CN111500561B (en) * 2020-05-08 2022-12-02 江南大学 Method for improving extraction efficiency of intracellular pullulanase
CN112526141A (en) * 2020-11-23 2021-03-19 福建傲农生物科技集团股份有限公司 Glutamine and method for simultaneously determining glutamine and asparagine in sample

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007125779A1 (en) * 2006-04-25 2007-11-08 Kikkoman Corporation Eukaryotic amadoriase having excellent thermal stability, gene and recombinant dna for the eukaryotic amadoriase, and process for production of eukaryotic amadoriase having excellent thermal stability
WO2011015326A2 (en) * 2009-08-03 2011-02-10 Roche Diagnostics Gmbh Fructosyl amino acid oxidase

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58110600A (en) 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd Recombinant plasmid containing human beta-interferon gene
ATE282692T1 (en) 1983-01-13 2004-12-15 Max Planck Gesellschaft NON-ONCOGENIC TI-PLASMID VECTOR SYSTEM AND RECOMBINANT DNA MOLECULES USABLE FOR INTRODUCING EXPRESSABLE GENES INTO PLANT GENOMS
ATE73845T1 (en) 1984-05-11 1992-04-15 Ciba Geigy Ag TRANSFORMATION OF PLANT HERITAGE.
JPS63233798A (en) 1986-10-09 1988-09-29 Kyowa Hakko Kogyo Co Ltd Production of 5'-guanylic acid
IL84459A (en) 1986-12-05 1993-07-08 Agracetus Apparatus and method for the injection of carrier particles carrying genetic material into living cells
EP0832981A1 (en) 1987-02-17 1998-04-01 Pharming B.V. DNA sequences to target proteins to the mammary gland for efficient secretion
JP2545078B2 (en) 1987-04-06 1996-10-16 協和醗酵工業株式会社 Method for producing nucleic acid-related substance
JP2928287B2 (en) 1988-09-29 1999-08-03 協和醗酵工業株式会社 Novel polypeptide
JPH0322979A (en) 1989-06-19 1991-01-31 Kyowa Hakko Kogyo Co Ltd Novel plasminogen-activation factor
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
JP3131322B2 (en) 1991-12-17 2001-01-31 協和醗酵工業株式会社 Novel α2 → 3 sialyltransferase
JPH063299A (en) 1992-06-22 1994-01-11 Showa Electric Wire & Cable Co Ltd Method for judging hardness of ultraviolet curing resin covering optical fiber
ATE398679T1 (en) 1992-07-07 2008-07-15 Japan Tobacco Inc METHOD FOR TRANSFORMING A MONOCOTYLEDON PLANT
JPH0670080A (en) 1992-08-17 1994-03-11 Toshiba Corp Facsimile character recognizing system
EP0643132B1 (en) 1993-03-29 2006-05-31 Kyowa Hakko Kogyo Co., Ltd. Alpha-1,3-fucosyltransferase
CA2664150C (en) 1996-09-17 2013-01-08 Kyowa Hakko Bio Co., Ltd. Processes for producing sugar nucleotides and complex carbohydrates
JP2000333696A (en) 1999-05-26 2000-12-05 Arkray Inc Measurement of glycosylated amine
JP3949854B2 (en) 1999-10-01 2007-07-25 キッコーマン株式会社 Method for measuring glycated protein
JP4231668B2 (en) 2001-09-04 2009-03-04 キッコーマン株式会社 Novel fructosyl peptide oxidase
DE60330376D1 (en) 2002-10-23 2010-01-14 Daiichi Pure Chemicals Co Ltd DEFRUCTOSYLIERUNGSVERFAHREN
JP4504923B2 (en) 2003-05-21 2010-07-14 旭化成ファーマ株式会社 Method for measuring hemoglobin A1c, enzyme used therefor and method for producing the same
JP5243878B2 (en) * 2008-08-04 2013-07-24 東洋紡株式会社 Fructosylvalylhistidine measuring enzyme and method of use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007125779A1 (en) * 2006-04-25 2007-11-08 Kikkoman Corporation Eukaryotic amadoriase having excellent thermal stability, gene and recombinant dna for the eukaryotic amadoriase, and process for production of eukaryotic amadoriase having excellent thermal stability
WO2011015326A2 (en) * 2009-08-03 2011-02-10 Roche Diagnostics Gmbh Fructosyl amino acid oxidase

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 15 May 2008 (2008-05-15), "Emericella nidulans amadoriase.", XP002671363, retrieved from EBI accession no. GSP:AOB50785 Database accession no. AOB50785 *
FUJIWARA M ET AL: "Alteration of substrate specificity of fructosyl-amino acid oxidase from Ulocladium sp. JS-103", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 102, no. 3, 1 September 2006 (2006-09-01), pages 241 - 243, XP025182876, ISSN: 1389-1723, [retrieved on 20060901], DOI: 10.1263/JBB.102.241 *
HYO-YOUNG JEONG ET AL: "The veA gene is necessary for the inducible expression by fructosyl amines of the Aspergillus nidulans faoA gene encoding fructosyl amino acid oxidase (amadoriase, EC 1.5.3)", ARCHIVES OF MICROBIOLOGY, vol. 178, no. 5, 1 November 2002 (2002-11-01), pages 344 - 350, XP055021595, ISSN: 0302-8933, DOI: 10.1007/s00203-002-0463-x *
KOZO HIROKAWA ET AL: "Enhancement of thermostability of fungal deglycating enzymes by directed evolution", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 78, no. 5, 1 February 2008 (2008-02-01), pages 775 - 781, XP019586340, ISSN: 1432-0614 *
See also references of WO2010041715A1 *
YOSHIDA NOBUYUKI ET AL: "Primary structures of fungal fructosyl amino acid oxidases and their application to the measurement of glycated proteins", EUROPEAN JOURNAL OF BIOCHEMISTRY, BLACKWELL PUBLISHING, BERLIN, DE, vol. 242, no. 3, 1 January 1996 (1996-01-01), pages 499 - 505, XP002561949, ISSN: 0014-2956, DOI: 10.1111/J.1432-1033.1996.0499R.X *

Also Published As

Publication number Publication date
KR20110069138A (en) 2011-06-22
EP2357228B1 (en) 2017-05-10
US20140057333A1 (en) 2014-02-27
CN102232111A (en) 2011-11-02
JP2015037402A (en) 2015-02-26
JP5665081B2 (en) 2015-02-04
JPWO2010041715A1 (en) 2012-03-08
HK1163734A1 (en) 2012-09-14
EP2357228A1 (en) 2011-08-17
CA2739881A1 (en) 2010-04-15
US20120003678A1 (en) 2012-01-05
CN102232111B (en) 2014-12-03
US8883142B2 (en) 2014-11-11
US20140234886A1 (en) 2014-08-21
US8304249B2 (en) 2012-11-06
WO2010041715A1 (en) 2010-04-15
US8790905B2 (en) 2014-07-29

Similar Documents

Publication Publication Date Title
EP2357228A4 (en) Novel fructosyl peptide oxidase
PL2462223T3 (en) Fructosyl amino acid oxidase
EP2089034A4 (en) Novel uses
IL208990A0 (en) Novel phenylimidazopyrazines
ZA201103171B (en) Novel uses
IL200863A0 (en) Novel uses
GB0920981D0 (en) Peptides
GB0801836D0 (en) Novel composition
ZA201102329B (en) Novel composition
GB0816976D0 (en) Protein
EP2123666A4 (en) Peptide
GB0818328D0 (en) Novel enzyme
GB0804721D0 (en) Enzyme
GB0803628D0 (en) Enzyme
ZA201103888B (en) Novel use
GB0804722D0 (en) Enzyme
GB0703945D0 (en) Peptide
GB0920987D0 (en) Peptides
EP2097439A4 (en) Novel peptides
GB0708529D0 (en) Peptide standards
GB0818627D0 (en) Protein
GB0817978D0 (en) Peptides
GB0800831D0 (en) Enzymes 1
GB0800414D0 (en) Novel use
AU2008100325A4 (en) Spapool bimini

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20110506

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20120327

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 1/19 20060101ALI20120316BHEP

Ipc: C12N 1/15 20060101ALI20120316BHEP

Ipc: C12N 1/21 20060101ALI20120316BHEP

Ipc: C12N 15/00 20060101AFI20120316BHEP

Ipc: C12N 9/04 20060101ALI20120316BHEP

Ipc: C12N 5/10 20060101ALI20120316BHEP

Ipc: G01N 33/72 20060101ALI20120316BHEP

Ipc: C12Q 1/26 20060101ALI20120316BHEP

Ipc: C12N 15/09 20060101ALI20120316BHEP

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 15/09 20060101ALI20120426BHEP

Ipc: G01N 33/72 20060101ALI20120426BHEP

Ipc: C12Q 1/26 20060101ALI20120426BHEP

Ipc: C12N 1/21 20060101ALI20120426BHEP

Ipc: C12N 1/15 20060101ALI20120426BHEP

Ipc: C12N 1/19 20060101ALI20120426BHEP

Ipc: C12N 15/00 20060101AFI20120426BHEP

Ipc: C12N 9/04 20060101ALI20120426BHEP

Ipc: C12N 5/10 20060101ALI20120426BHEP

17Q First examination report despatched

Effective date: 20130620

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20161118

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 892366

Country of ref document: AT

Kind code of ref document: T

Effective date: 20170515

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602009046062

Country of ref document: DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20170510

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 9

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 892366

Country of ref document: AT

Kind code of ref document: T

Effective date: 20170510

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170811

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170810

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170910

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170810

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602009046062

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20180213

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171008

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171031

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171031

REG Reference to a national code

Ref country code: BE

Ref legal event code: MM

Effective date: 20171031

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171031

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 10

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171008

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171008

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20091008

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170510

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170510

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20200930

Year of fee payment: 12

Ref country code: FR

Payment date: 20200914

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20200911

Year of fee payment: 12

Ref country code: DE

Payment date: 20200922

Year of fee payment: 12

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 602009046062

Country of ref document: DE

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20211008

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20211008

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20220503

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20211031

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20211008