EP2334802A4 - METHODS OF GENERATING SPECIFIC LIBRARIES OF GENES - Google Patents

METHODS OF GENERATING SPECIFIC LIBRARIES OF GENES

Info

Publication number
EP2334802A4
EP2334802A4 EP09813548A EP09813548A EP2334802A4 EP 2334802 A4 EP2334802 A4 EP 2334802A4 EP 09813548 A EP09813548 A EP 09813548A EP 09813548 A EP09813548 A EP 09813548A EP 2334802 A4 EP2334802 A4 EP 2334802A4
Authority
EP
European Patent Office
Prior art keywords
methods
gene specific
specific libraries
generating gene
generating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09813548A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP2334802A1 (en
Inventor
Christopher K Raymond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of EP2334802A1 publication Critical patent/EP2334802A1/en
Publication of EP2334802A4 publication Critical patent/EP2334802A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP09813548A 2008-09-09 2009-09-09 METHODS OF GENERATING SPECIFIC LIBRARIES OF GENES Withdrawn EP2334802A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9557908P 2008-09-09 2008-09-09
PCT/US2009/056380 WO2010030683A1 (en) 2008-09-09 2009-09-09 Methods of generating gene specific libraries

Publications (2)

Publication Number Publication Date
EP2334802A1 EP2334802A1 (en) 2011-06-22
EP2334802A4 true EP2334802A4 (en) 2012-01-25

Family

ID=42005453

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09813548A Withdrawn EP2334802A4 (en) 2008-09-09 2009-09-09 METHODS OF GENERATING SPECIFIC LIBRARIES OF GENES

Country Status (3)

Country Link
EP (1) EP2334802A4 (zh)
CN (1) CN102203273A (zh)
WO (1) WO2010030683A1 (zh)

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KR101797773B1 (ko) 2009-01-30 2017-11-15 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 시퀀싱에서 핵산 구축물용 어댑터
WO2011156529A2 (en) * 2010-06-08 2011-12-15 Nugen Technologies, Inc. Methods and composition for multiplex sequencing
CN102409408B (zh) * 2010-09-21 2013-08-07 深圳华大基因科技服务有限公司 一种利用微量基因组dna进行全基因组甲基化位点精确检测的方法
CN102409043B (zh) * 2010-09-21 2013-12-04 深圳华大基因科技服务有限公司 高通量低成本Fosmid文库构建的方法及其所使用标签和标签接头
WO2012175013A1 (zh) * 2011-06-24 2012-12-27 深圳华大基因科技有限公司 用于确定人体具有异常状态的系统和方法
AU2012288629B2 (en) * 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
CN103975075A (zh) * 2011-11-24 2014-08-06 深圳华大基因科技有限公司 一种检测病毒在待测样本中整合方式的探针及其制备方法和应用
WO2013112923A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP2861787B1 (en) 2012-06-18 2017-09-20 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CN107090491A (zh) * 2012-11-05 2017-08-25 鲁比康基因组学公司 条形编码核酸
EP2964779B1 (en) 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
CA2938910A1 (en) * 2014-02-11 2015-08-20 F. Hoffmann-La Roche Ag Targeted sequencing and uid filtering
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
AU2015280069B2 (en) * 2014-06-23 2021-08-12 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
SG11201700891SA (en) 2014-08-06 2017-03-30 Nugen Technologies Inc Digital measurements from targeted sequencing
AU2014406026B2 (en) * 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
CN107208313B (zh) * 2014-12-02 2022-01-07 特里比奥迪卡有限责任公司 用于治疗诊断应用的方法和试剂盒
US10429342B2 (en) 2014-12-18 2019-10-01 Edico Genome Corporation Chemically-sensitive field effect transistor
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
CA2971791A1 (en) * 2014-12-22 2016-06-30 Veterinaerinstituttet Salmon gill poxvirus
CN104946639B (zh) * 2015-07-01 2017-10-31 益善生物技术股份有限公司 构建基因突变测序文库的引物和方法以及试剂盒
EP3397379A4 (en) * 2015-12-30 2019-05-29 Bio-Rad Laboratories, Inc. PREPARATION OF SEPARATE PCR BANK IN DROPLETS
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
JP6860662B2 (ja) * 2016-10-31 2021-04-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ キメラ生成物の同定のためのバーコードを付けられた環状ライブラリーの構築
JP2020504709A (ja) * 2016-11-18 2020-02-13 ツイスト バイオサイエンス コーポレーション 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN109750092B (zh) 2017-11-03 2022-12-06 北京贝瑞和康生物技术有限公司 一种靶向富集高gc含量目标dna的方法和试剂盒
CN108004301B (zh) * 2017-12-15 2022-02-22 格诺思博生物科技南通有限公司 基因目标区域富集方法及建库试剂盒
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2612412A (en) * 2020-01-31 2023-05-03 Avida Biomed Inc Systems and methods for targeted nucleic acid capture
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统

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EP1184466A2 (en) * 2000-08-26 2002-03-06 Affymetrix, Inc. Target nucleic acid enrichment and amplification for array analysis
WO2002083943A2 (de) * 2001-04-12 2002-10-24 Epigenomics Ag Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen
WO2007057652A1 (en) * 2005-11-15 2007-05-24 Solexa Limited Method of target enrichment

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US20060051789A1 (en) * 2004-07-01 2006-03-09 Somagenics, Inc. Methods of preparation of gene-specific oligonucleotide libraries and uses thereof
DK1924704T3 (da) * 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion

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EP1184466A2 (en) * 2000-08-26 2002-03-06 Affymetrix, Inc. Target nucleic acid enrichment and amplification for array analysis
WO2002083943A2 (de) * 2001-04-12 2002-10-24 Epigenomics Ag Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen
WO2007057652A1 (en) * 2005-11-15 2007-05-24 Solexa Limited Method of target enrichment

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OKOU DAVID T ET AL: "Microarray-based genomic selection for high-throughput resequencing", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 4, no. 11, 1 October 2007 (2007-10-01), pages 907 - 909, XP002528498, ISSN: 1548-7091, DOI: 10.1038/NMETH1109 *
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Also Published As

Publication number Publication date
CN102203273A (zh) 2011-09-28
WO2010030683A1 (en) 2010-03-18
EP2334802A1 (en) 2011-06-22

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