EP2334802A4 - Verfahren zur erstellung genspezifischer bibliotheken - Google Patents

Verfahren zur erstellung genspezifischer bibliotheken

Info

Publication number
EP2334802A4
EP2334802A4 EP09813548A EP09813548A EP2334802A4 EP 2334802 A4 EP2334802 A4 EP 2334802A4 EP 09813548 A EP09813548 A EP 09813548A EP 09813548 A EP09813548 A EP 09813548A EP 2334802 A4 EP2334802 A4 EP 2334802A4
Authority
EP
European Patent Office
Prior art keywords
methods
gene specific
specific libraries
generating gene
generating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09813548A
Other languages
English (en)
French (fr)
Other versions
EP2334802A1 (de
Inventor
Christopher K Raymond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of EP2334802A1 publication Critical patent/EP2334802A1/de
Publication of EP2334802A4 publication Critical patent/EP2334802A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP09813548A 2008-09-09 2009-09-09 Verfahren zur erstellung genspezifischer bibliotheken Withdrawn EP2334802A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9557908P 2008-09-09 2008-09-09
PCT/US2009/056380 WO2010030683A1 (en) 2008-09-09 2009-09-09 Methods of generating gene specific libraries

Publications (2)

Publication Number Publication Date
EP2334802A1 EP2334802A1 (de) 2011-06-22
EP2334802A4 true EP2334802A4 (de) 2012-01-25

Family

ID=42005453

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09813548A Withdrawn EP2334802A4 (de) 2008-09-09 2009-09-09 Verfahren zur erstellung genspezifischer bibliotheken

Country Status (3)

Country Link
EP (1) EP2334802A4 (de)
CN (1) CN102203273A (de)
WO (1) WO2010030683A1 (de)

Families Citing this family (40)

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EP2391732B1 (de) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Verfahren die adaptoren zur nukleinsaure constructen für transmembrane sequenzierung benutzen
EP2580378A4 (de) * 2010-06-08 2014-01-01 Nugen Technologies Inc Verfahren und zusammensetzung für multiplex-sequenzierung
CN102409043B (zh) * 2010-09-21 2013-12-04 深圳华大基因科技服务有限公司 高通量低成本Fosmid文库构建的方法及其所使用标签和标签接头
CN102409408B (zh) * 2010-09-21 2013-08-07 深圳华大基因科技服务有限公司 一种利用微量基因组dna进行全基因组甲基化位点精确检测的方法
CN103797130B (zh) * 2011-06-24 2016-07-27 深圳华大基因科技有限公司 用于确定人体具有异常状态的系统和方法
IN2014DN00221A (de) * 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
CN103975075A (zh) * 2011-11-24 2014-08-06 深圳华大基因科技有限公司 一种检测病毒在待测样本中整合方式的探针及其制备方法和应用
CA2862552A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014071361A1 (en) * 2012-11-05 2014-05-08 Rubicon Genomics Barcoding nucleic acids
WO2014135838A1 (en) 2013-03-08 2014-09-12 Oxford Nanopore Technologies Limited Enzyme stalling method
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CN110819621B (zh) * 2014-02-11 2024-03-26 豪夫迈·罗氏有限公司 靶向测序和uid过滤
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN106604994B (zh) * 2014-06-23 2021-12-14 通用医疗公司 通过测序评估的DSBs的全基因组无偏鉴定(GUIDE-Seq)
US10102337B2 (en) 2014-08-06 2018-10-16 Nugen Technologies, Inc. Digital measurements from targeted sequencing
EP3192869B1 (de) * 2014-09-12 2019-03-27 MGI Tech Co., Ltd. Isoliertes oligonukleotid und verwendung davon bei der nukleinsäuresequenzierung
JP6910295B2 (ja) * 2014-12-02 2021-07-28 トリビオティカ・エルエルシー 診断治療融合的な応用のための方法及びキット
WO2016100049A1 (en) 2014-12-18 2016-06-23 Edico Genome Corporation Chemically-sensitive field effect transistor
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
EP3237607B1 (de) * 2014-12-22 2020-05-06 Veterinærinstituttet Lachskiemenpockenvirus
CN104946639B (zh) * 2015-07-01 2017-10-31 益善生物技术股份有限公司 构建基因突变测序文库的引物和方法以及试剂盒
EP3397379A4 (de) * 2015-12-30 2019-05-29 Bio-Rad Laboratories, Inc. Erstellung von auf tröpfchenpartitionierter pcr basierender bibliothek
EP3459115A4 (de) 2016-05-16 2020-04-08 Agilome, Inc. Graphen-fet-vorrichtungen, systeme und verfahren zur verwendung davon zur sequenzierung von nukleinsäuren
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2018077847A1 (en) * 2016-10-31 2018-05-03 F. Hoffmann-La Roche Ag Barcoded circular library construction for identification of chimeric products
CA3044057A1 (en) * 2016-11-18 2018-05-24 Twist Bioscience Corporation Polynucleotide libraries having controlled stoichiometry and synthesis thereof
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN109750092B (zh) 2017-11-03 2022-12-06 北京贝瑞和康生物技术有限公司 一种靶向富集高gc含量目标dna的方法和试剂盒
CN108004301B (zh) * 2017-12-15 2022-02-22 格诺思博生物科技南通有限公司 基因目标区域富集方法及建库试剂盒
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
EP4097231A4 (de) * 2020-01-31 2024-04-03 Agilent Technologies Inc Systeme und verfahren zum gezielten nukleinsäureeinfang

Citations (3)

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Publication number Priority date Publication date Assignee Title
EP1184466A2 (de) * 2000-08-26 2002-03-06 Affymetrix, Inc. Anreicherung und Amplifikation von Ziel-Nukleinsäuren für Array-Analyse
WO2002083943A2 (de) * 2001-04-12 2002-10-24 Epigenomics Ag Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen
WO2007057652A1 (en) * 2005-11-15 2007-05-24 Solexa Limited Method of target enrichment

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WO2002034949A2 (en) * 2000-10-27 2002-05-02 Molecular Staging Inc. Methods for identifying genes associated with diseases or specific phenotypes
GB0114853D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Happier Mapping
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WO2006007569A2 (en) * 2004-07-01 2006-01-19 Somagenics, Inc. Methods of preparation of gene-specific oligonucleotide libraries and uses thereof
EP1924704B1 (de) * 2005-08-02 2011-05-25 Rubicon Genomics, Inc. Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
EP1184466A2 (de) * 2000-08-26 2002-03-06 Affymetrix, Inc. Anreicherung und Amplifikation von Ziel-Nukleinsäuren für Array-Analyse
WO2002083943A2 (de) * 2001-04-12 2002-10-24 Epigenomics Ag Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen
WO2007057652A1 (en) * 2005-11-15 2007-05-24 Solexa Limited Method of target enrichment

Non-Patent Citations (4)

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Title
JUDY ST. JOHN ET AL: "Rapid capture of DNA targets", BIOTECHNIQUES, vol. 44, no. 2, 1 February 2008 (2008-02-01), pages 259 - 264, XP055014746, ISSN: 0736-6205, DOI: 10.2144/000112633 *
OKOU DAVID T ET AL: "Microarray-based genomic selection for high-throughput resequencing", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 4, no. 11, 1 October 2007 (2007-10-01), pages 907 - 909, XP002528498, ISSN: 1548-7091, DOI: 10.1038/NMETH1109 *
See also references of WO2010030683A1 *
UNN HILDE REFSETH ET AL: "HYBRIDIZATION CAPTURE OF MICROSATELLITES DIRECTLY FROM GENOMIC DNA", ELECTROPHORESIS, WILEY INTERSCIENCE, DE, vol. 18, no. 9, 1 January 1997 (1997-01-01), pages 1519 - 1523, XP002952426, ISSN: 0173-0835, DOI: 10.1002/ELPS.1150180905 *

Also Published As

Publication number Publication date
CN102203273A (zh) 2011-09-28
WO2010030683A1 (en) 2010-03-18
EP2334802A1 (de) 2011-06-22

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