EP2334435A2 - Système modulaire d'unités fonctionnelles pour mélanger, traiter et/ou séparer des échantillons pour une utilisation dans la recherche biologique/médicale et dans le diagnostic - Google Patents

Système modulaire d'unités fonctionnelles pour mélanger, traiter et/ou séparer des échantillons pour une utilisation dans la recherche biologique/médicale et dans le diagnostic

Info

Publication number
EP2334435A2
EP2334435A2 EP09806070A EP09806070A EP2334435A2 EP 2334435 A2 EP2334435 A2 EP 2334435A2 EP 09806070 A EP09806070 A EP 09806070A EP 09806070 A EP09806070 A EP 09806070A EP 2334435 A2 EP2334435 A2 EP 2334435A2
Authority
EP
European Patent Office
Prior art keywords
separation
mixing
mixing cylinder
functional units
flow
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09806070A
Other languages
German (de)
English (en)
Inventor
Jan-Michael Heinrich
Christoph Mohr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitaet Leipzig
Original Assignee
Universitaet Leipzig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitaet Leipzig filed Critical Universitaet Leipzig
Publication of EP2334435A2 publication Critical patent/EP2334435A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B01L3/5635—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors connecting two containers face to face, e.g. comprising a filter
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00002—Chemical plants
    • B01J2219/00004—Scale aspects
    • B01J2219/00011—Laboratory-scale plants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00—Enclosures; Chambers
    • B01L1/52—Transportable laboratories; Field kits
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/028—Modular arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Definitions

  • the aim of the invention is therefore to provide a system of functional units, which can be combined depending on the methodological task, preferably mated, and which allow a simple and reproducible mixing, physical and / or chemical reactions and separation of sample media.
  • system consisting of one or more mixing cylinders, one or more separation devices and a flow-regulating connection unit, which are combined with one another in an application-specific manner for the most diverse requirements, has proven to be particularly simple and simple reproducible system for processing sample media.
  • the invention accordingly relates to a system of freely combinable functional units which is suitable in particular for mixing, processing and / or separating samples
  • a flow-regulating connection unit (5) wherein the flow-regulating connection unit (5) is preferably formed as a vacuum unit, wherein the system is designed so that the functional units (2; 3; 5), preferably via simple connectors, in any order , In particular with any number of functional units, are connected to a preferably self-contained, sample processing system.
  • the functional units according to the invention therefore preferably consist of or are
  • the system allows the use of different cylindrical (closed on one side) containers, such as sample tubes. This is done by scaling the Dimensions achieved.
  • the cylinder-like containers can be used as a collection or waste container for the supernatants or the respective fractions.
  • connection is preferably achieved by a wedge-shaped geometry.
  • at least one seal in particular a line seal and preferably propylene, is used.
  • Variant one is straightened in the lower part of the wedge, so that a circumferential connection between two functional units comes to conditions.
  • variant one has an easier to produce by injection molding geometry.
  • Variant two is a circumferential ring at the end of the plug-in connection, which is preferably attached directly to the spline connection by injection molding. This leads to an additional ring connection.
  • this seal is possible to produce as an O-ring or by 2K injection molding.
  • An inclined plane on the top of the separation units (backwash level) allows a directed backwashing of the retentate from the filter surface while reducing the amount of backwash liquid.
  • the backwashing level according to the invention is preferably an arrangement of several, preferably 6-18, webs which are arranged on the outside of the separating unit (3) or protrude from the separating unit (3), the webs being formed in this way or arranged so that the underside of the webs at one in relation to the top and / or bottom of the separation unit (imaginary) oblique (essay) plane ends or through the bottom of the webs one, compared to the top or bottom of the Separation unit (3), (imaginary) inclined (essay) level when placing the separation unit (3) is formed on a collecting container (7).
  • the slope of the (imaginary) plane is preferably formed over an angle of 2-45 °, preferably 5-40 °.
  • spacers (32) prevent the separating device (3) during the backwashing with the wall of the collecting container (7), which is preferably formed as a tube, comes into contact.
  • the mixing cylinder is preferably open on both sides, but can also be open only on one side (so that one would tip the sample material on the separating device), and is on the one side by a lid which preferably has a living hinge, or as a screw cap, plug or pluggable Cover executed on the lid, and on the other side closed by a peelable and / or pierceable foil and / or a pluggable, pluggable or screw-on bottom cap.
  • the mixing cylinder is used for sample collection and can be used as an alternative to single-sided open sample tubes.
  • the interior is designed so that it can serve as a mixing vessel for achieving biological, chemical or physical reactions.
  • the geometry of the mixing cylinder ensures
  • Both sides of the mixing cylinder are designed with different geometry: - filling side (but can also be used to dump the liquid on the separating device):
  • the filling side is closed by a lid with a film hinge (for other closure options see above). From here, the filling of the mixing cylinder with sample material and optional reaction material and solutions.
  • the film hinge allows aseptic working.
  • the lid has a peripheral edge. The peripheral edge avoids snagging or sticking of the cylinder during the mixing process (in particular on a tilting roller mixer roller mixer) to an unobstructed rotation of the To ensure mixing cylinder with film hinge, preferably the lid is additionally modified with a peripheral edge.
  • - Discharge side can also be used as a filling side:
  • the discharge side of the mixing cylinder is designed so that it can be plugged into the separation device, stuffed or screwed with this.
  • the drainage side can optionally be closed with a foil and / or bottom cap (see above: preferably by inserting plugs or screws).
  • the foil one-time use
  • the foil is attached to the cylinder by heat (thermowelded). If necessary (e.g., after incubation with fabrics), the film is removed by peeling off through the attached protrusion or by a sharp object such as e.g. a pipette tips, or pierced by a puncture device directly to the severing device / perforated.
  • the use of a bottom cap allows aseptic work.
  • the entire fitting area / insertion area of the mixing cylinder is closed by the bottom cap.
  • the discharge side of the mixing cylinder can be used simultaneously as the filling side.
  • the discharge side can optionally be equipped directly with a sieve, which allows, for example, the pre-separation of desired or the deposition of undesirable fractions. Retained desired fractions can be used directly or reused by resealing the mixing cylinder with the bottom cap for further biological, chemical or physical reactions.
  • the design of the cylinder, when combined with a sieve on the discharge side allows it to be combined directly with the flow regulating connection unit, and thus can be used similarly to the separation device. Retained unwanted fractions increase the purity of the subsequent separation with the separation device.
  • each side can be used as a filling and / or emptying side, wherein the filling and / or emptying side with a lid, in particular with one of the above-mentioned lid, eg with a losierbaren cover, are formed ,
  • the direction for combination with a separating device is freely selectable.
  • Each of the sides can be connected to the severing device.
  • the separation devices are based on the principle of filtration.
  • the filter material and the pore size are provided according to the task. It can serve both separation by size separation, as well as the immobilization of substances.
  • Flow regulating connection unit (adapter), which is preferably formed as a vacuum unit.
  • Vacuum unit in the sense of the invention means in particular a transition piece with (laterally arranged) intake, preferably for the connection with a vacuum pump, so that by connecting the vacuum unit (5) to the separation vessel (7), eg Screwing / clamping the vacuum unit (5) on or with the separation vessel (7), a suction bottle is made.
  • the flow-regulating connection unit allows the connection of the separation device with different cylindrical (closed on one side) containers, such as sample tubes and serves as an adapter.
  • the shape is correspondingly adapted on one side to the geometry of the separating device and on the opposite side to the geometry of the respective cylinder-like containers.
  • the connection unit is responsible for a defined liquid flow and supports the separation unit in the application of at least one, preferably more than two, separation device (s). By closing the connection unit, liquid can be kept in the separation device for incubation purposes (eg for immunochemical reactions). After opening the closure of the connection unit, the liquid flows into the cylinder-like container.
  • the target substance can either be in the run, immobilized on the membrane or attached to solid supports which have been introduced into the reaction space of the separation device.
  • the connection unit can be connected to the collection and / or waste vessel either via a thread or via a wedge construction.
  • the tubes differ greatly in the increase of the thread, but the inner diameter of the tubes only varies by a few micrometers. This can preferably be compensated by a simple wedge construction of the vacuum unit. The fixation preferably takes place as a "press fit.” This results in a system which offers the user the opportunity to use his existing tubes.
  • connection unit allows the combination with a vacuum filtration by connection of vacuum generating devices and equipment combinations (among other things disposable syringes, peristaltic pump, vacuum pump, water jet pump, pipettor with hose etc. Vacuum pressure, vacuum generating devices)
  • vacuum generating devices and equipment combinations among other things disposable syringes, peristaltic pump, vacuum pump, water jet pump, pipettor with hose etc. Vacuum pressure, vacuum generating devices
  • Figure 4E and 4F Principle of vacuum filtration briefly explained and serves as an example for the use of vacuum generating devices and device combinations.
  • the vacuum unit was designed so that a liquid movement takes place exclusively via the movement of the disposable syringe (57). Since the system is closed, no liquid from the mixing cylinder (2) on the separation unit (3) can run into the collecting vessel (7).
  • the membrane material used serves as an additional barrier and prevents the compensation of air from the collecting vessel against the liquid in the receiving vessel. When the syringe is drawn up, a negative pressure forms in the collecting vessel, which conveys the liquid from the collecting vessel via the separating membrane into the collecting vessel.
  • the two check valves (56) allow the air to be pushed out of the spray without the air being forced back into the separation units.
  • Figure 1A shows a 3D representation of a system according to the invention with a mixing cylinder (2) with film hinge lid, two separation units or
  • Figure 1 B shows a side view of the system shown in Figure 1A.
  • Figure 1C shows a side sectional view of the system according to Figure 1A
  • Figure 1D shows a view of the system according to Fig. 1A from above.
  • Figure 1 E shows a view of the system as shown in Fig. 1A from below.
  • Figure 2A shows a side view of the mixing cylinder (2) with open
  • Figure 2B shows a side view of the mixing cylinder (2) with the lid closed.
  • Figure 2C shows a rear view of the mixing cylinder (2) with closed
  • Figure 2D shows a sectional view of the mixing cylinder (2) with the lid open
  • Figure 2E shows a sectional view of the mixing cylinder (2) with closed
  • Figure 2F shows a view of the mixing cylinder (2) with the lid open from below.
  • Figure 2G shows a view of the mixing cylinder (2) with the lid open from the top.
  • Figure 2H shows a view of the mixing cylinder (2) with the lid open from below.
  • Figure 2I shows a view of the mixing cylinder (2) with the lid open from below.
  • Figure 3A shows a 3D representation of the separation device (3).
  • Figure 3B shows a side view of the separating device (3).
  • Figure 3C shows a front view of the separator (3).
  • Figure 3D shows a sectional view of the separating device (3).
  • Figure 3E shows a top view of the separator (3).
  • Figure 3F shows a view of the separator (3) from below.
  • Figure 3G shows a line seal variant of the separator (3).
  • Figure 3H shows another line seal variant of the separator (3).
  • Figure 31 shows a composite of two separation units (3), wherein preferably the upper of the two separation units (3) has a filter or a separation membrane with a pore diameter x and the lower of the two separation units (3) has a filter or a separation membrane with a Pore diameter ⁇ x so that a sieve cascade is made.
  • Figure 3K shows a sectional view of the two separation units (3) with variants of the line seal.
  • Figure 3L shows an illustration of a separation unit (3) with a backwash level, which allows directional backflushing.
  • Backwashing device for backwashing the retentate after use of the system according to the invention, and after the separation unit (3) of the other
  • Functional units of the modular system has been separated and with a further receptacle (6), e.g. a centrifuge tube (6) has been connected.
  • a further receptacle (6) e.g. a centrifuge tube (6) has been connected.
  • Figure 3N shows a side view of the arrangement according to Fig. 3M.
  • Figure 30 shows a sectional view of the arrangement according to Fig. 3M.
  • Figure 4A shows a 3D representation of the vacuum unit (5).
  • Figure 4C shows a sectional view of the vacuum unit (5).
  • Figure 4D shows a top view of the vacuum unit (5).
  • Figure 4E shows a system according to the invention with connection of a
  • Vacuum device - before filtration Vacuum device - before filtration.
  • Figure 4F shows a system according to the invention with connection of a
  • Vacuum device - after filtration Vacuum device - after filtration.
  • Figure 4G shows a structure of a system according to the invention for detaching cells on the sieve surface of the separation unit (3).
  • Figure 4H shows a sectional view of a structure for detaching cells from the membrane surface of the separation unit (3), in particular the construction according to FIG
  • a flow-regulating connection unit which is preferably formed as a vacuum unit (5) is designed so that the functional units, preferably via connectors, in any order, in particular with any number of functional units, are connected to a, preferably self-contained, sample processing system, in particular for mixing, editing and / or separating samples.
  • the system is preferably formed, in particular in the dimensions of its functional units, so that it can be brought onto or be connected to commercially available containers, in particular laboratory tubes, preferably with a volume of 5-100 ml, preferably a filling volume of 15 ml or 50 ml can.
  • the interior of the mixing cylinder or cylinders is designed in such a way that optimum mixing of the samples is made possible.
  • the system is designed so that the separation device (s) on the principle of filtration consists or exist, ie in particular that the separation device (s) (each) a filter, a membrane and / or a sieve have or have or are configured with it.
  • the filters, membranes and / or screens preferably have pores with a pore diameter of 10 nm-1 mm.
  • the at least one separating device is designed so that membranes can be used for separating the material, which have a pore diameter between 10 nm and 1 mm.
  • the membrane (s) consist of known material (s) which can be used both as size filters and / or as solid carriers with immobilizing surface design ,
  • Figure 3D Section view of a separator
  • Figure 3K Sectional view of the 2 separation devices with variants of the
  • Figure 30 Sectional view of the structure for backwashing the retentate
  • Figure 4A 3D representation of the vacuum unit
  • Figure 4B Side view of the vacuum unit
  • Figure 4C Sectional view of the vacuum unit
  • Figure 4D Top view of the vacuum unit
  • Figure 5B Sectional view of the bottom cap fixation
  • Figure 5C Sectional view of the required geometry on the mixing cylinder for the

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Le mélange, les réactions physiques et/ou chimiques et la séparation sont des étapes fondamentales dans la recherche et le diagnostic biologiques. Notamment la recherche requiert des systèmes de laboratoires spécifiques aux tâches n'étant pas disponibles dans le commerce en raison de leur faible diffusion et de leur utilisation spécifique. Face à l'absence de ces systèmes, la résolution de ces tâches nécessite d'improviser au moyen des récipients, filtres, centrifugeuses etc. disponibles. L'invention vise à proposer un système d'unités fonctionnelles pouvant être assemblées en fonction des processus imposés par les tâches. Le système selon l'invention est constitué d'unités fonctionnelles pouvant être combinées librement, et se compose de cylindres de mélange, de dispositifs de séparation et d'une unité de connexion à régulation du flux, de préférence conçue en tant qu'unité à vide. Le système est conçu de telle manière que les unités fonctionnelles sont connectées, au moyen de connexions par enfichage, dans un ordre quelconque et dans une quantité d'unités fonctionnelles quelconque, de manière à former un système de traitement d'échantillons fermé.
EP09806070A 2008-10-06 2009-10-06 Système modulaire d'unités fonctionnelles pour mélanger, traiter et/ou séparer des échantillons pour une utilisation dans la recherche biologique/médicale et dans le diagnostic Withdrawn EP2334435A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008050750A DE102008050750A1 (de) 2008-10-06 2008-10-06 Baukastensystem zum methodenorientierten Zusammenstecken von Funktionseinheiten zum Mischen, Bearbeiten und/oder Trennen von Proben zur Anwendung in der biologischen/medizinischen Forschung und für die Diagnostik
PCT/DE2009/001411 WO2010040347A2 (fr) 2008-10-06 2009-10-06 Système modulaire d'unités fonctionnelles pour mélanger, traiter et/ou séparer des échantillons pour une utilisation dans la recherche biologique/médicale et dans le diagnostic

Publications (1)

Publication Number Publication Date
EP2334435A2 true EP2334435A2 (fr) 2011-06-22

Family

ID=41795142

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09806070A Withdrawn EP2334435A2 (fr) 2008-10-06 2009-10-06 Système modulaire d'unités fonctionnelles pour mélanger, traiter et/ou séparer des échantillons pour une utilisation dans la recherche biologique/médicale et dans le diagnostic

Country Status (4)

Country Link
US (1) US20110287472A1 (fr)
EP (1) EP2334435A2 (fr)
DE (2) DE102008050750A1 (fr)
WO (1) WO2010040347A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202014105965U1 (de) 2014-12-10 2015-01-26 Cell.Copedia GmbH Spritzengehäuse zum Pipettieren eines biologischen Materials mit integrierten Membranen
USD886320S1 (en) * 2019-04-29 2020-06-02 Wavesense, Inc. Fluid sequestration apparatus
GB201907537D0 (en) 2019-05-29 2019-07-10 Waterford Institute Of Tech Modular sample processing device

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3539300A (en) * 1967-10-23 1970-11-10 Schering Corp Body fluid collector and separator having improved flow rate
FR2536671B1 (fr) * 1982-11-26 1988-06-10 Sartorius Gmbh Appareil de filtrage pour liquides, de type statique a membrane
US4871662A (en) * 1986-08-11 1989-10-03 Watertest Corporation Device for shipping microbiology test samples
US4797259A (en) * 1986-12-15 1989-01-10 Pall Corporation Well-type diagnostic plate device
US5624638A (en) * 1993-05-05 1997-04-29 Davcotech, Inc. Modular laboratory equipment and coupling system
US5601711A (en) * 1994-10-31 1997-02-11 Gelman Sciences Inc. Selective separation filter device
US6090572A (en) * 1998-06-26 2000-07-18 Biostar, Incorporated Filtration and extraction device and method of using the same
US6458278B1 (en) * 1999-02-22 2002-10-01 Nalge Nunc International Corporation Filtering unit having separately attachable filter cassette, filter cassette, and method of filtering
EP1990092B1 (fr) * 2001-03-09 2010-02-10 Gen-Probe Incorporated Capot pénétrable

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2010040347A2 *

Also Published As

Publication number Publication date
DE112009003386A5 (de) 2015-03-05
DE102008050750A1 (de) 2010-04-08
WO2010040347A2 (fr) 2010-04-15
US20110287472A1 (en) 2011-11-24
WO2010040347A4 (fr) 2010-08-26
WO2010040347A3 (fr) 2010-06-17

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