Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
  • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
  • C07D471/04—Ortho-condensed systems
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P37/00—Drugs for immunological or allergic disorders
  • A61P37/08—Antiallergic agents

Definitions

• the present invention relates to novel compounds of Formula (I), or salts thereof, processes for their preparation, intermediates usable in these processes, and pharmaceutical compositions containing the compounds or salts.
• the invention also relates to the use of these compounds or salts thereof in therapy, for example as inhibitors of phosphodiesterase type IV (PDE4) and as antagonists of muscarinic acetylcholine receptors (mAChRs), and useful in the treatment of, and/or prophylaxis of respiratory diseases, including anti-inflammatory and allergic diseases such as chronic obstructive pulmonary disease (COPD), asthma, rhinitis (e.g. allergic rhinitis), atopic dermatitis or psoriasis.
• COPD chronic obstructive pulmonary disease
• COPD chronic obstructive pulmonary disease
• rhinitis e.g. allergic rhinitis
• atopic dermatitis or psoriasis e.g. allergic rhinitis
• Muscarinic acetylcholine receptors belong to the superfamily of G-protein coupled receptors having seven transmembrane domains. There are five subtypes of mAChRs, termed M1-M5, and each is the product of a distinct gene. Each of these five subtypes displays unique pharmacological properties.
• Muscarinic acetylcholine receptors are widely distributed in vertebrate organs, and these receptors can mediate both inhibitory and excitatory actions. For example, in smooth muscle found in the airways, M3 mAChRs mediate contractile responses. For a review, see Caulfield (1993 Pharmac. Ther. 58:319-79).
• mAChRs have been localized to smooth muscle in the trachea and bronchi, the submucosal glands, and the parasympathetic ganglia. Muscarinic receptor density is greatest in parasympathetic ganglia and then decreases in density from the submucosal glands to tracheal and then bronchial smooth muscle. Muscarinic receptors are nearly absent from the alveoli.
• M3 mAChRs located on airway smooth muscle, mediate muscle contraction. Stimulation of M3 mAChRs activates the enzyme phospho lipase C via binding of the stimulatory G protein Gq/11 (Gs), leading to liberation of phosphatidyl inositol-4,5-bisphosphate, resulting in phosphorylation of contractile proteins. M3 mAChRs are also found on pulmonary submucosal glands. Stimulation of this population of M3 mAChRs results in mucus secretion.
• M2 mAChRs make up approximately 50-80% of the cholinergic receptor population on airway smooth muscles. Although the precise function is still unknown, they inhibit catecholaminergic relaxation of airway smooth muscle via inhibition of cAMP generation.
• Neuronal M2 mAChRs are located on postganglionic parasympathetic nerves. Under normal physiologic conditions, neuronal M2 mAChRs provide tight control of acetylcholine release from parasympathetic nerves. Inhibitory M2 mAChRs have also been demonstrated on sympathetic nerves in the lungs of some species. These receptors inhibit release of noradrenaline, thus decreasing sympathetic input to the lungs.
• Ml mAChRs are found in the pulmonary parasympathetic ganglia where they function to enhance neurotransmission. These receptors have also been localized to the peripheral lung parenchyma, however their function in the parenchyma is unknown. Muscarinic acetylcholine receptor dysfunction in the lungs has been noted in a variety of different pathophysiological states. In particular, in asthma and chronic obstructive pulmonary disease (COPD), inflammatory conditions lead to loss of inhibitory M2 muscarinic acetylcholine autoreceptor function on parasympathetic nerves supplying the pulmonary smooth muscle, causing increased acetylcholine release following vagal nerve stimulation (Fryer et al. 1999 Life Sci 64 (6- 7) 449-55). This mAChR dysfunction results in airway hyperreactivity and hyperresponsiveness mediated by increased stimulation of M3 mAChRs.
• COPD chronic obstructive pulmonary disease
• Recent literature has focused on the non-neuronal cholinergic system in the lungs where there is an emerging literature supporting a role for muscarinic receptors in mediating immunomodulatory and inflammatory functions in respiratory diseases such as asthma and COPD.
• Many of the components for cholinergic signaling have been reported to be contained within inflammatory and resident cells of the lungs, including muscarinic receptor expression on lymphocytes, alveolar macrophages, mast cells and epithelial cells.
• acetylcholine is solely a neurotransmitter of the parasympathetic nervous system is currently being challenged as there is mounting evidence to suggest it has an integral role in host defense and airway inflammation.
• COPD chronic bronchitis
• chronic bronchiolitis chronic bronchiolitis
• emphysema a major cause of mortality and morbidity in the world.
• Smoking is the major risk factor for the development of
• COPD nearly 50 million people in the U.S. alone smoke cigarettes, and an estimated 3,000 people take up the habit daily.
• COPD is expected to rank among the top five as a world- wide health burden by the year 2020.
• Inhaled anti-cholinergic therapy is currently considered the "gold standard" as first line therapy for COPD (Pauwels et al. 2001 Am. J. Respir. Crit. Care Med. 163: 1256-1276).
• relatively few anti-cholinergic compounds are available for use in the clinic for pulmonary indications.
• Ipratropium Bromide (Atrovent ⁇ ; and Combivent ⁇ , in combination with albuterol) is one of the few inhaled anti-cholinergic marketed for the treatment of airway hyperreactive diseases. While this compound is a potent anti- muscarinic agent, it is short acting, and thus must be administered as many as four times daily in order to provide relief for the COPD patient.
• the long-acting anti-cholinergic Tiotropium Bromide (Spiriva ⁇ ) has recently been approved in a number of countries.
• mAChRs are widely distributed throughout the body, the ability to apply anticholinergics locally and/or topically to the respiratory tract is particularly advantageous, as it would allow for lower doses of the drug to be utilized. Furthermore, the ability to design topically active drugs that have long duration of action, and in particular, are retained either at the receptor or by the lung, would allow the avoidance of unwanted side effects that may be seen with systemic anti-cholinergic use.
• WO 2004/091482 describes a dimeric bicyclic amine derivative having anti-muscarinic receptor activity:
• X represents a group of the formula (d) or (e): — Y-Ar-Y- — Y-L-Y-
• Y is selected from the group consisting of a bond, OR , SR , NR R , and C 1.4 alkyl; and L represents a bond, C1.4 alkyl or C3_g cycloalkyl.
• WO 2005/095407 also discloses a similar dimeric bicyclic amine derivative to that above having anti-muscarinic receptor activity wherein inter alia, X is a group of the formula (d), (e) and
• Y is, independently, selected from the group consisting of a bond, O, S, NR 2 , -NR 2 Cj.4 alkyl- , and Cj.4 alkyl- ; each of the alkyl groups may contain a heteroatom selected from O, NR 2 , or S; and
• Z represents a bond, O, NR 2 , S, Cj.4 alkylidene or Cj.4 alkyl.
• Other mAChR antagonists, non-dimeric in structure, may be found in WO 2004/012684;
• NVA237 glycopyrrolate or glycopyrronium bromide, a quaternary ammonium derivative with anticholinergic and antimuscarinic properties. It is being developed by Novartis for once daily treatment of COPD.
• LAS-34273 also known as aclidinium bromide, is a quaternary ammonium anticholinergic muscarinic M3 antagonist originated by Almirall and believed to be in phase 3 development for treating COPD.
• the compounds are disclosed as central nervous system depressants useful as ataractic, analgesic and hypotensive agents.
• US 3,925,388, US 3,856,799, US 3,833,594 and US 3,755,340 disclose 4-amino derivatives of pyrazolo[3,4-b]pyridine-5-carboxylic acids and esters.
• the compounds are mentioned as being central nervous system depressants useful as ataractic agents or tranquilizers, as having anti-inflammatory and analgesic properties.
• the compounds are mentioned as increasing the intracellular concentration of adenosine-3',5'-cyclic monophosphate and for alleviating the symptoms of asthma.
• the compound tracazolate ethyl 4-(n-butylamino)-l-ethyl-6-methyl-lH-pyrazolo[3,4-b]- pyridine-5-carboxylate
• anxiolytic agent e.g. see J.B. Patel et al., Eur. J. Pharmacol, 1982, 78, 323
• Other 1 -substituted 4-(NH 2 orNH-alkyl)-lH-pyrazolo[3,4-b]- pyridine-5-carboxylic acid esters and amides are disclosed as potential anxiolytic agents in T.M. Bare et al., J. Med. Chem., 1989, 32, 2561-2573.
• CA 1003419, CH 553 799 and T.Denzel, Archiv der Pharmazie, 1974, 307(3), 177-186 disclose 4,5-disubstituted lH-pyrazolo[3,4-b]pyridines unsubstituted at the 1 -position.
• JP-2002-20386-A (Ono Yakuhin Kogyo KK) published on 23 January 2002 discloses pyrazolopyridine compounds of the following inter alia formula:
• JP-2002-20386-A are stated as having PDE4 inhibitory activity and as being useful in the prevention and/or treatment of inflammatory diseases and many other diseases.
• l,3-Dimethyl-4-(arylamino)-pyrazolo[3,4-b]pyridines with a 5-C(O)NH 2 substituent similar or identical to those in JP-2002-20386-A were disclosed as orally active PDE4 inhibitors by authors from Ono Pharmaceutical Co. in: H. Ochiai et al., Bioorg. Med. Chem. Lett., 2004, vol. 14(1) and Pp. 29-32. Full papers on these and similar compounds as orally active PDE4 inhibitors are: H. Ochiai et al., Bioorg. Med.
• EP 0 076 035 Al discloses pyrazolo[3,4-b]pyridine derivatives as central nervous system depressants useful as tranquilizers or ataractic agents for the relief of anxiety and tension states.
• WO 02/060900 A2 appears to disclose, as MCP- 1 antagonists for treatment of allergic, inflammatory or autoimmune disorders or diseases, a series of bicyclic heterocyclic compounds with a -C(O)-NR 4 -C(O)-NR 5 R 6 substituent, including isoxazolo[5,4-b]pyridines and lH-pyrazolo[3,4-b]pyridines (named as pyrazolo[5,4-b]pyridines) with the
• WO 00/15222 discloses inter alia pyrazolo[3,4-b]pyridines having inter alia a C(O)-Xj group at the 5-position and a group Ej at the 4-position of the ring system.
• Xj can for example be -OR9, -N(Rg)(Rj Q) or -N(R5)(-A2-R2), and Ej can for example be -NH-Aj -eye loalkyl, -NH-Aj -substituted cycloalkyl, or -NH-Aj -heterocyclo; wherein Aj is an alkylene or substituted alkylene bridge of 1 to 10 carbons and A2 can for example be a direct bond or an alkylene or substituted alkylene bridge of 1 to 10 carbons.
• the compounds are disclosed as being useful as inhibitors of cGMP phosphodiesterase, especially PDE type V, and in the treatment of various cGMP-associated conditions such as erectile dysfunction.
• H. de Mello, A. Echevarria, et al., J. Med. Chem., 2004, 47(22), 5427-5432 discloses 3- methyl or 3-phenyl 4-anilino-lH-pyrazolo[3,4-b]pyridine 5-carboxylic esters as potential anti- Leishmania drugs.
• NRyRy a group (R ⁇ a is preferably H) and with a group Het at the 5-position of the pyrazolo[3,4- b]pyridine, wherein Het is usually a 5-membered optionally substituted heteroaryl group.
• WO 2004/056823 Al also discloses the use of these compounds as PDE4 inhibitors and for the treatment and/or prophylaxis of inter alia COPD, asthma or allergic rhinitis.
• WO 2004/024728 A2 discloses pyrazolo[3,4-b]pyridine having the following generic formula.
• WO 2004/024728 A2 pyrazolo[3,4-b]pyridine compounds are disclosed as being inhibitors of PDE4.
• WO 2004/024728 and WO 2004/056823 are noted in Expert Opin. Ther. Patents, 2005 (January edition), 15(1), 111-114.
• WO 2005/058892 Al discloses pyrazolo[3,4-b]pyridine compounds for use as PDE4 inhibitors for treating inflammatory or allergic diseases such as COPD, asthma, rheumatoid arthritis, allergic rhinitis or atopic dermatitis.
• WO 03/087064 is directed to compounds having both antagonism of the M3 muscarinic receptor and inhibition of PDE4, having the formula:
• the present invention provides for the novel compounds of Formula (I), and pharmaceutical compositions comprising a compound of Formula (I) and a pharmaceutically acceptable carrier or diluent.
• R4 a is hydrogen or C 1-2 alkyl
• R5 a is hydrogen or Cl .4 alkyl; n is an integer having a value of 1, 2 or 3; v is 0 to 4;
• RI is selected from the group consisting of C ⁇ alkyl, -CH2-C 1 ⁇ fluoroalkyl, and -CH2CH2OH
• R 2 is selected from the group consisting of hydrogen, Cj _4alkyl, Cj _2fluoroalkyl, cyclopropyl, cyclobutyl, and (cyclopropyl)methyl-;
• R ⁇ is selected from the group consisting of an optionally substituted Czj. ⁇ cycloalkyl, an optionally substituted mono-unsaturated-C5_7cycloalkenyl, an optionally substituted heterocyclic group of sub-formula (aa), (bb) and (cc), and a bicyclic group of sub-formula (dd), and (ee);
• R 10a is hydrogen, methyl, C(O)NH 2 , C(O)-methyl, or C(O)-C j fluoroalkyl;
• Y 1 , Y 2 and Y 3 are independently CH 2 or oxygen, provided that no more than one of Y 1 , Y 2 and
• R ⁇ cycloalkyl ring is deemed to be the connection point to the -NH- in formula (I), that is the ring atom connecting to the -NH- in formula (I)); and wherein, when R ⁇ is the optionally substituted heterocyclic group of sub-formula (aa), (bb) or
• Ar 1 and Ar 2 are independently selected from the group consisting of an optionally substituted phenyl and an optionally substituted monocyclic heteroaryl;
• Rg is NR ⁇ Rg, or is a heterocyclic group of the subformula (ff), (gg), (hh), (ii), (jj), (kk), (11), (mm) or (nn):
• R-6 is an optionally substituted C 5 -C 7 membered ring containing one or two nitrogens, or a corresponding bicyclic ring containing one or two nitrogens, l-azabicyclo[2.2.2]oct-3-yl; or 8-methyl-8-azabicyclo[3.2.1]oct-3-yl;
• R9 is selected from the group consisting of hydrogen, optionally substituted C 1-6 alkyl, optionally substituted aryl, optionally substituted arylC j -2alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl Cj -2alkyl, optionally substituted heterocyclic, optionally substituted heterocyclic Cj -2alkyl, and C(O) Cj-2alkyl;
• R9a is selected from the group consisting of hydrogen, optionally substituted C J -6 alkyl, optionally substituted aryl, optionally substituted arylC j -2alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl C j ⁇ alkyl, optionally substituted heterocyclic, optionally substituted heterocyclic Cj-2alkyl, and C(O)C j-2alkyl;
• Rd is independently selected at each occurrence from the group consisting of hydrogen, hydroxy, optionally substituted C J -6 alkyl, amino, optionally substituted aryl, optionally substituted arylCj-2alkyl, optionally substituted heterocyclic, optionally substituted heterocyclicC j -2alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl
• Ri 5 and Ri6 are independently selected at each occurrence from the group consisting of hydrogen, and C j -4 alkyl;
• Ri 7 is selected at each occurrence from the group consisting of optionally substituted C J .4 alkyl, optionally substituted C3-7 cycloalkyl, optionally substituted C 3 _ 7 cycloalkylCj-4alkyl, optionally substituted aryl, optionally substituted arylC j ⁇ alkyl, heterocyclic, optionally substituted heterocyclic, optionally substituted heterocyclicC j ⁇ alkyl, optionally substituted heteroaryl, and optionally substituted heteroaryl Cj-4alkyl;
• Rb is independently selected at each occurrence from the group consisting of hydrogen, optionally substituted C I -4 alkyl, optionally substituted Ci- ⁇ cycloalkyl, optionally substituted Ci- ⁇ cycloalkylCi-4 alkyl, Q-4 alkoxy, NR 15 R 16 C l-4alkyl,
• Rc is independently selected at each occurrence from the group consisting of hydrogen and C 1-4 alkyl;
• Re and Rf are each independently selected at each occurrence from the group consisting of hydrogen and C I -4 alkyl;
• RlO is independently selected at each occurrence from the group consisting of hydrogen and C 1-4 alkyl
• Rl 3a is hydrogen, or Ci -2 alkyl;
• X is (C(Ri 3)) p , or (CR 6 Re) 81 - X 2 -(CR f R f ) s2 ;
• X 2 is NRi 3a, O, S(0)m, or C(O); m is O, 1, or 2; p is 1 or 2; q is O, 1 or 2; s is O, 1 or 2; si is O to 2; s2 is 0 to 2, provided that when R ⁇ is a heterocyclic group of the subformulas (ff), (ii), (jj) and (11), and X 2 is NRi3 a , O, or S(0)m and m is 0 or 1, then s2 is 1 or 2, or X is (CH(Ri 3 ))p; t is 1 to 4; tl is 0 to 4; R4 and R5 are each independently selected from the group consisting of hydrogen, optionally substituted Cl .4 alkyl, optionally substituted C3-C7 cycloalkyl, optionally substituted C3-C7 cycloalkyl C 1-4 alkyl, optionally substituted heterocyclic, optionally substituted heterocyclic C
• R7 is selected from hydrogen, or an optionally substituted C 1.4 alkyl
• R8 is (CRdlRdl)t - NRi 1R12 or (CRdI RdI )ti - RuJ
• RdI is independently at each occurrence selected from the group consisting of hydrogen, optionally substituted C 1-4 alkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocyclic;
• Ri 4 is selected from the group consisting of C 1-4 alkyl, C3-C6 cycloalkyl, optionally substituted heterocyclic, and optionally substituted heteroaryl moiety; and Rl 1 and Ri 2 are independently selected from the group consisting of hydrogen and C 1-4 alkyl; or a pharmaceutically acceptable salt thereof.
• This invention provides for a method of treating both a muscarinic acetylcholine receptor (mAChR) mediated disease, wherein acetylcholine binds to an M3 mAChR and a phosphodiesterase type IV (PDE4) mediated disease, whereby the compound also binds to the PDE4 isotype, which comprises administering an effective amount of a compound of Formula (I) or a pharmaceutically acceptable salt thereof to a mammal in need thereof.
• mAChR muscarinic acetylcholine receptor
• PDE4 phosphodiesterase type IV
• One use of compounds of Formula (I), or pharmaceutically acceptable salt thereof are in the treatment and/or prophylaxis of an inflammatory and/or allergic disease in a mammal.
• One or more specific compounds within the presently invented compounds may be suitable for use as dual PDE4/mAChR inhibitors via an inhaled route of administration.
• Compounds of the present invention provide for a single compound which has the attributes of each pharmacophore optimized for each molecular target in a balanced fashion. This resulting in vivo profile allows for efficacy and duration of action at both targets, inhibition of PDE4 and antagonism of the mAChR, in a defined dose range. It is now possible to produce a compound which is developable to treat at least two aspects of complex disease etiology, for example bronchoconstriction and inflammation found in diseases such as COPD and asthma.
• the present invention is directed to a concept of having dual pharmacophores in one molecule that retains potency across both pharmacological groups. Another aspect of the invention is that in addition to retaining dual pharmacological activity the compounds are developable for commercial activities.
• the compound may be administered to a mammal in needed thereof, suitably one to four times daily, and preferably either a once or a twice daily treatment.
• the compound is administered topically or by inhalation (via nose or mouth) for use in the treatment and/or prophylaxis of a disease for which either pharmacophore has previously been associated with treatment of a PDE4 or an M3 mediated disease.
• this will be an inflammatory and/or allergic disease, such as the treatment of COPD, asthma, adult respiratory distress syndrome, rhinitis, allergic rhinitis, atopic dermatitis, urticaria, allergic conjunctivitis, psoriasis, ulcerative colitis, or Crohn's disease.
• One or more specific compounds within the presently invented compounds may be suitable for use as dual PDE4/mAChR inhibitors via an inhaled route of administration.
• One or more specific compounds within the presently invented compounds may be suitable for use as dual PDE4/mAChR inhibitors via an intranasal route of administration.
• One or more specific compounds within the presently invented compounds may be suitable for use as dual PDE4/mAChR inhibitors via a topical route of administration.
• R ⁇ is suitably selected from Cj_3alkyl, -CH2-C1 _2fluoroalkyl, or -CH2CH2OH.
• RMs suitably selected from Cj _3alkyl, such as methyl, ethyl, n-propyl, or isopropyl.
• R ⁇ is ethyl.
• R ⁇ is hydrogen, C j _4alkyl, such as methyl, ethyl, n-propyl, isopropyl, or n-butyl, a Cj _2fluoroalkyl, cyclopropyl, cyclobutyl, or (cyclopropyl)methyl-.
• R ⁇ is methyl, ethyl, n-propyl, isopropyl, or n-butyl.
• R ⁇ is ethyl.
• R ⁇ is optionally substituted Czj. ⁇ cycloalkyl, or optionally substituted mono-unsaturated-C5_7cycloalkenyl, or an optionally substituted heterocyclic group of sub- formula (aa), (bb) or (cc), or a bicyclic group of sub-formula (dd), or (ee);
• n ⁇ and n 2 are independently 1 or 2.
• Y is O, S, SO2, or NRlOa J n one embodiment of the invention Y is O.
• R 1Oa is a hydrogen atom (H), methyl, C(O)NH2, C(O)-methyl, or C(O)-C j fluoroalkyl.
• Y 1 , Y 2 and Y 3 are each independently selected from CH2 or oxygen, provided that no more than one of Y 1 , Y 2 and Y 3 are oxygen.
• R ⁇ is an optionally substituted Czj. ⁇ cycloalkyl
• R ⁇ is the optionally substituted heterocyclic group of sub-formula (aa), (bb) or (cc)
• R ⁇ is optionally substituted mono-unsaturated-C5_7cycloalkenyl
• the cycloalkenyl is optionally substituted on a ring carbon with one substituent being fluoro or methyl, and the R- ⁇ ring carbon bonded to the -NH- group of formula (I) does not partake in the cycloalkenyl double bond.
• Ry is the heterocyclic group of sub-formula (aa) and Y is NR 10
• R 10 is not C(O)-methyl, or C(O)-C j fluoroalkyl
• R 3 is the heterocyclic group of sub-formula (bb) and Y is NRI O, then RIO is not methyl
• R 3 is the heterocyclic group of sub-formula (cc)
• Y is O, S, SO2 or NR ⁇ wherein R ⁇ is H or methyl.
• any -C(O)NHR 24 or -C(O)R 25 substituent on a ring carbon is: at the 3-position of a R 3 cyclobutyl ring; or at the 3- or 4- position of a R 3 cyclopentyl ring; or at the 4-position of a R 3 cyclohexyl ring; or at the 3-, A-, 5- or 6- position of a R 3 cycloheptyl ring wherein, in this connection, the 1 -position of the R 3 cycloalkyl ring is deemed to be the connection point to the -NH- in formula (I), that is the ring atom connecting to the -NH- in formula (I).
• any OH, methoxy, fluoroalkoxy, -CH 2 OH, -CH(Me)OH, -CH 2 CH 2 OH, -CH 2 NH 2 , or -C(O)OH substituent on a ring carbon is: at the 3-position of a R 3 cyclobutyl ring; or at the 3- or 4- position of a R 3 cyclopentyl ring; or at the
• R 3 is the sub-formula (bb) and (cc). In another embodiment of the invention R 3 is the sub-formula (bb) and (cc), and nl and n 2 independently are 1 or 2. In another embodiment, Y is O, and nl and n 2 are 1.
• R 3 is the sub-formula (bb). In another embodiment R 3 is the sub-formula (bb), and Y is O. In yet another embodiment R 3 is the sub-formula (bb), Y is O, and nl is 1.
• R 43 is hydrogen, methyl or ethyl. In one embodiment of the invention R 43 is hydrogen or methyl. In another embodiment of the invention R 43 is hydrogen.
• R 5a is hydrogen, methyl or ethyl. In one embodiment of the invention R 5a is hydrogen.
• v is O to 5. In one embodiment, v is O or 1. In another embodiment v is 1 and R5a is hydrogen.
• Ari and Ar 2 are independently selected from the group consisting of an optionally substituted phenyl and an optionally substituted monocyclic heteroaryl. In one embodiment, Ari and Ar 2 are independently selected from an optionally substituted aryl. In another embodiment both Ari and Ar 2 are independently selected from an optionally substituted phenyl.
• Ari and Ar 2 are independently substituted one or more times, suitably 1 to 4 times, at each occurrence by halogen, such as fluorine, chlorine, bromine or iodine; cyano; hydroxy; hydroxy substituted Ci-4alkyl; C 1-4 alkoxy, such as methoxy or ethoxy; S(O) m ' Ci-IO alkyl, wherein m' is 0, 1 or 2, such as methyl thio, methyl sulfmyl or methyl sulfonyl; amino, a mono or di-substituted Ci-2alkyl amino; Ci-4alkyl, such as methyl, ethyl, propyl, isopropyl, or t-butyl; C2-4alkyl alkenyl, such as ethenyl, 1 -propenyl, 2-propenyl, or 2-methyl- 1 -propenyl; or a halosubstituted C I -4 alkyl, such CH
• the optional substituents are independently selected from halogen, alkyl, alkoxy, or cyano. In another embodiment the optional substituents are independently selected from fluorine, chlorine, methyl, methoxy or cyano.
• suitable heteroaryl rings for Ari and Ar 2 include, but are not limited to, furyl, pyranyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, isoxazolyl, isothiazolyl, imidazolyl, pyrazolyl, oxadiazolyl, oxathiadiazolyl, triazolyl, tetrazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, and triazinyl.
• the heteroaryl ring is a pyridine.
• Ari and Ar 2 are both independently selected from an optionally substituted aryl, preferably an optionally substituted phenyl. In one embodiment of the invention both Ar 1 and Ar 2 are independently selected from optionally substituted phenyls
• the Ar2 ring is phenyl.
• the ArI ring is a heteroaryl ring. In another embodiment the ArI ring is a pyridine ring.
• the ArI ring is a phenyl optionally substituted one or more times independently by halogen, alkyl, alkoxy, or cyano. In another embodiment the ArI ring is a phenyl optionally substituted one or more times independently by fluorine, chlorine, methyl, methoxy or cyano.
• the Ar2 ring is phenyl
• the ArI ring is a phenyl optionally substituted one or more times independently by halogen, alkyl, alkoxy, or cyano.
• the Ari ring is mono-substituted in the 5- or in the 6-position. In another embodiment if the Ari ring is di-substituted it is substituted in both the 5 and 6-position. In one embodiment, the Ari ring is an optionally substituted phenyl ring. In another embodiment the phenyl ring is substituted one or more times, suitably 1 to 2 times, by halogen, cyano, or C I -4 alkoxy. In another embodiment, the Ari ring is a phenyl, or an optionally substituted phenyl in the 6-position, such as by fluorine, or methoxy.
• R ⁇ is NR ⁇ Rg, or is a heterocyclic group of the subformula (ff), (gg), (hh), (ii), (jj), (kk), (ll), (mm) or (nn):
• R6 is an optionally substituted C5-C7 membered ring containing one or two nitrogens, or a corresponding bicyclic ring containing one or two nitrogens.
• R ⁇ is an optionally substituted C5-C7 membered ring containing one or two nitrogens, or a corresponding bicyclic ring containing one or two nitrogens
• the rings include variations of the ring nitrogen positions from subformulas (ff) to (nn).
• the nitrogens are at the 1-4 position
• other options include 1-3, or 1-2 nitrogens with similarly substituted Ra, Rb, RbI, R9, etc. substituents.
• s is 0, or is an integer having a value of 1 or 2. In one embodiment of the invention s is 1 or 2. In another embodiment of the invention s is 1.
• R ⁇ is a heterocyclic group of the sub formula (ft), and s is 1 or 2. In another embodiment, R ⁇ is a heterocyclic group of the sub formula (ft), s is 1 or 2, and Rb is independently selected from hydrogen, or methyl.
• R ⁇ is a heterocyclic group of the subformula (jj).
• R7 is selected from hydrogen, or an optionally substituted C j -4 alkyl. In one embodiment of the invention R7 is hydrogen or methyl.
• R 8 is (CRdI RdI )t - NR11R12 or (CRdI RdI )ti - Ri 4 -
• RdI is independently at each occurrence selected from the group consisting of hydrogen, optionally substituted C j -4 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclic.
• t is 1 to 4. In one embodiment, t is 1 or 2.
• tl is 0 to 4. In one embodiment, tl is 0, or 1. In another embodiment, tl is 0.
• Ri 1 and Ri 2 are independently selected from hydrogen, or C 1-4 alkyl.
• R 14 is selected from Cl .4 alkyl, C3-C5 cycloalkyl, optionally substituted aryl, optionally substituted heterocyclic, or optionally substituted heteroaryl moiety.
• R M is a heterocyclic group of the subformula (ft), (ii), (jj), (11), (mm) and (nn), then tl is other than 0.
• R M when R M is a heteroaryl it is a monocyclic five- to seven- membered unsaturated hydrocarbon ring containing at least one heteroatom selected from oxygen, nitrogen and sulfur; or a fused C8-C12 aromatic ring comprising at least one heteroatom selected from oxygen, nitrogen and sulfur.
• heteroaryl rings include, but are not limited to, furyl, pyranyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, isoxazolyl, isothiazolyl, imidazolyl, pyrazolyl, oxadiazolyl, oxathiadiazolyl, triazolyl, tetrazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, uracil, indolyl, isoindolyl, indazolyl, indolizinyl, azaindolyl, benzoxazolyl, benzimidazolyl, benzothiazolyl, benzofuranyl, benzothiophenyl, quinolyl, isoquinolyl, quinazolinyl, quinoxalinyl, naphthyridin
• R 14 when R 14 is an optionally substituted heteroaryl it is selected from an optionally substituted thiophenyl, optionally substituted pyridinyl, or an optionally substituted pyrimidinyl.
• R 14 when R 14 is a heterocyclic it is a C3-C7 monocyclic non-aromatic hydrocarbon ring containing at least one heteroatom selected from nitrogen, oxygen, sulphur or oxidized sulphur moieties, such as S(0)m, and m is 0 or an integer having a value of 1 or 2, or the heterocyclic is a fused, C8-C12 saturated or partially unsaturated ring system wherein one of the rings may be aromatic, or heteroaromatic.
• Each of the fused rings may have from four to seven ring atoms.
• suitable heterocyclyl groups include, but are not limited to, the saturated or partially saturated versions of the heteroaryl moieties as defined above, such as tetrahydropyrrole, tetrahydropyran, tetrahydrofuran, tetrahydrothiophene (including oxidized versions of the sulfur moiety), azepine, diazepine, aziridinyl, pyrrolinyl, pyrrolidinyl, 2-oxo- 1 -pyrrolidinyl, 3-oxo-l- pyrrolidinyl, l,3-benzdioxol-5-yl, imidazolinyl, imidazolidinyl, indolinyl, pyrazolinyl, pyrazolidinyl, piperidinyl, piperazinyl, morpholino and thiomorpholino (including oxidized versions of the sulfur
• R 14 is an optionally substituted heterocyclic ring
• the ring is an optionally substituted piperidinyl, piperazinyl, optionally substituted oxohexahydro-lH-azepine, or an optionally substituted 3'-[(l-Azabicyclo-[2.2.2]oct-3- yi.
• R 14 when R 14 is a C3-C5 cycloalkyl it is suitably selected from cyclopropyl, cyclopentyl, or cyclohexyl. In another embodiment when R 14 is a Cl .4 alkyl it is ethyl, isopropyl, n-propyl, n-butyl, sec-butyl, or t-butyl.
• Rd is independently at each occurrence selected from the group consisting of hydrogen, optionally substituted Cl .4 alkyl, optionally substituted aryl, optionally substituted heteroaryl, and an optionally substituted heterocyclic moiety.
• Rd is an optionally substituted moiety, excluding hydrogen, the moiety may be substituted one or more times, suitably 1 to 4 times, independently by halogen, such as fluorine or chlorine, or a C 1 -2alkyl.
• Rd is independently hydrogen or methyl.
• R9 is hydrogen, optionally substituted C 1-6 alkyl, optionally substituted aryl, optionally substituted arylCi ⁇ alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl Cj ⁇ alkyl, optionally substituted heterocyclic, optionally substituted heterocyclic Cj-2alkyl, or C(O) Cj ⁇ alkyl.
• R9 is an optionally substituted C 1-6 alkyl
• the alkyl is substituted one or more times, suitably 1 or 2 times independently by halogen, hydroxy, N R 4 5R 1 6, C 1-4 alkoxy, S(0)qCi-4 alkyl.
• R9 is hydrogen or methyl.
• R9 a is hydrogen, optionally substituted C 1-6 alkyl, optionally substituted aryl, optionally substituted arylCi ⁇ alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl Cj ⁇ alkyl, optionally substituted heterocyclic, optionally substituted heterocyclic Ci"2alkyl, C(O)C 1 -2alkyl.
• R9 a is hydrogen or optionally substituted Cl .3 alkyl.
• R a is independently hydrogen, or methyl.
• R a is independently hydrogen, or methyl.
• Rb is independently selected from hydrogen or methyl.
• Rt ⁇ l is independently selected from hydrogen or methyl.
• Rd is an optionally substituted C 1-6 alkyl
• the alkyl is substituted one or more times, suitably 1 or 2 times independently by halogen, hydroxy, NRi 5 Ri 6 , C 1-4 alkoxy, S(0)qCi-4 alkyl.
• R ⁇ is hydrogen or methyl.
• R c is independently selected at each occurrence from hydrogen or C 1-4 alkyl.
• RlO is independently selected from hydrogen or C 1-4 alkyl.
• Ri 5 and Ri 6 are independently selected from hydrogen, or C 1-4 alkyl. In one embodiment of the invention Ri 5 and Ri 6 are hydrogen or methyl.
• Ri 7 is selected from optionally substituted C 1-4 alkyl, optionally substituted C3-7 cycloalkyl, optionally substituted C 3 _ 7 cycloalkylCi-4alkyl, optionally substituted aryl, optionally substituted arylCi ⁇ alkyl, heterocyclic, optionally substituted heterocyclic, optionally substituted heterocyclicC i"4alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl Ci- 4 alkyl.
• X is (C(Ri 3 ))p, or (CR 6 R e ) 8I - X 2 -(CR f R f ) s2 .
• X 2 is NRi 3 a , O, S(0)m, or C(O).
• Rl3a is selected from hydrogen, Ci- 2 alkyl.
• Ri 3 is hydrogen.
• si is O to 2. In one embodiment of the invention si is O.
• s2 is O to 2.
• R ⁇ is a heterocyclic group of the sub formulas (ff), (ii), (jj) and (11), and X 2 is NRi3 a , O, or S(0)m (and m is O or 1) then s2 is 1 or 2, or X is the group (CH(Ri3))p.
• p is 1 or 2.
• q is O, 1 or 2.
• n 1, 2 or 3.
• n 3 is 1 to 3.
• m is 0, 1, or 2.
• R4 and R5 are independently selected from the group consisting of hydrogen, optionally substituted Cj _4alkyl, optionally substituted C3-C7 cycloalkyl, optionally substituted C3-C7 cycloalkyl Cj ⁇ alkyl, optionally substituted heterocyclic, optionally substituted heterocyclic Cj_4alkyl, optionally substituted C24 alkenyl, optionally substituted aryl, optionally substituted aryl C j _4alkyl optionally substituted heteroaryl, and optionally substituted heteroaryl Cj. 4 alkyl.
• R5 is hydrogen, and n is 1.
• R4 is hydrogen, or Cj_4alkyl.
• R4 and R5 are both hydrogen, and n is 1.
• R 1 is C j . 4 alkyl
• R 2 is a C j . 4 alkyl
• R3 is a morpholino
• Z2 and Z3 are independently at each occurrence selected from the group consisting of hydrogen, halogen, cyano and Cj_4alkoxy; n is 0 or an integer having a value of 1 or 2; v is 0 to 4;
• R4 a is hydrogen or C J -2 alkyl
• R-5a is hydrogen or C J .4 alkyl
• R4 is selected from the group consisting of hydrogen, optionally substituted C J .4 alkyl, optionally substituted C3-C7 cycloalkyl, optionally substituted C3-C7 cycloalkyl C J .4 alkyl, optionally substituted heterocyclic, optionally substituted heterocyclic Cj .4 alkyl, optionally substituted alkenyl, optionally substituted aryl, optionally substituted arylC J .4 alkyl optionally substituted heteroaryl, and optionally substituted heteroaryl C J .4 alkyl;
• R6 is NR7R8, or is a heterocyclic group of the subformula (ff), (gg), (hh), (ii), (jj), (kk), (11), (mm) or (nn):
• Rg is an optionally substituted C5-C7 membered ring containing one or two nitrogens, or a corresponding bicyclic ring containing one or two nitrogens;
• R9 is selected from the group consisting of hydrogen, optionally substituted C 1-6 alkyl, optionally substituted aryl, optionally substituted arylC j -2alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl Cj -2alkyl, optionally substituted heterocyclic, optionally substituted heterocyclic Cj -2alkyl, and C(O) Cj ⁇ alkyl;
• R9a is selected from the group consisting of hydrogen, optionally substituted C J -6 alkyl, optionally substituted aryl, optionally substituted arylC j -2alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl Cj-2alkyl, optionally substituted heterocyclic, optionally substituted heterocyclic Cj ⁇ alkyl, and C(O)C j ⁇ alkyl;
• Ri 5 and Ri6 are independently selected at each occurrence from hydrogen, or C J .4 alkyl;
• Ri 7 is selected at each occurrence from the group consisting of optionally substituted C 1-4 alkyl, optionally substituted C3-7 cycloalkyl, optionally substituted C 3 _ 7 cycloalkylCi-4alkyl, optionally substituted aryl, optionally substituted arylCj -4alkyl, heterocyclic, optionally substituted heterocyclic, optionally substituted heterocyclicCi ⁇ alkyl, optionally substituted heteroaryl, and optionally substituted heteroaryl C j -4alkyl;
• R7 is selected from hydrogen, or an optionally substituted C 1-4 alkyl
• R8 is (CRdlRdl)t - NRi 1R12 or (CRdlRdl)ti - Ri 4 ;
• RdI is independently at each occurrence selected from the group consisting of hydrogen, optionally substituted C 1-4 alkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocyclic; or
• Ri 4 is selected from the group consisting of C 1-4 alkyl, C3-C5 cycloalkyl, optionally substituted heterocyclic, and optionally substituted heteroaryl moiety; Rl 1 and Ri 2 are independently selected from hydrogen, or C 1-4 alkyl; or a pharmaceutically acceptable salt thereof. It is to be understood that the present invention covers all combinations of particular and preferred groups described hereinabove. It is also to be understood that the present invention encompasses compounds in which a particular group or parameter, e.g. S(0)m, etc. may occur more than once. In such compounds it will be appreciated that each group or parameter is independently selected from the values listed. When any variable occurs more than one time in a formula, its definition on each occurrence is independent of its definition at every other occurrence.
• Particular compounds according to the invention include those mentioned in the examples and their pharmaceutically acceptable derivatives.
• the term "pharmaceutically acceptable” means a compound which is suitable for pharmaceutical and veterinary usage. Salts and solvates of compounds of the invention which are suitable for use in medicine are those wherein the counter- ion or associated solvent is pharmaceutically acceptable. However, salts and solvates having non-pharmaceutically acceptable counter- ions or associated solvents are within the scope of the present invention, for example, for use as intermediates in the preparation of other compounds of the invention and their pharmaceutically acceptable salts and solvates.
• pharmaceutically acceptable derivative means any pharmaceutically acceptable salt, solvate or prodrug e.g.
• ester of a compound of the invention, which upon administration to the recipient is capable of providing (directly or indirectly) a compound of the invention, or an active metabolite or residue thereof.
• Such derivatives are recognizable to those skilled in the art, without undue experimentation. Nevertheless, reference is made to the teaching of Burger's Medicinal Chemistry and Drug Discovery, 5 th Edition, Vol. 1 : Principles and Practice, which is incorporated herein by reference to the extent of teaching such derivatives.
• pharmaceutically acceptable derivatives are salts, solvates, esters, carbamates and phosphate esters.
• pharmaceutically acceptable derivatives are salts, solvates and esters.
• pharmaceutically acceptable derivatives are salts and esters, in particular salts.
• the compounds of the present invention may be in the form of and/or may be administered as a pharmaceutically acceptable salt.
• suitable salts see Berge et al., J. Pharm. Sci., 1977, 66, 1-19.
• a pharmaceutical acceptable salt may be readily prepared by using a desired acid or base as appropriate.
• the salt may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent.
• Salts of the compounds of the present invention may, for example, comprise acid addition salts resulting from reaction of an acid with a nitrogen atom present in a compound of formula (I). Salts encompassed within the term "pharmaceutically acceptable salts" refer to non-toxic salts of the compounds of this invention.
• Suitable addition salts are formed from acids which form nontoxic salts and examples are acetate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, calcium edetate, camsylate, carbonate, chloride, clavulanate, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, ethanesulphonate, formate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydrogen phosphate, hydroiodide, hydroxynaphthoate, iodide, isethionate, lactate, lactobionate, laurate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate
• Pharmaceutically acceptable base salts include ammonium salts such as a trimethylammonium salt, alkali metal salts such as those of sodium and potassium, alkaline earth metal salts such as those of calcium and magnesium and salts with organic bases, including salts of primary, secondary and tertiary amines, such as isopropylamine, diethylamine, ethanolamine, trimethylamine, dicyclohexyl amine and N-methyl-D-glucamine.
• ammonium salts such as a trimethylammonium salt
• alkali metal salts such as those of sodium and potassium
• alkaline earth metal salts such as those of calcium and magnesium
• salts with organic bases including salts of primary, secondary and tertiary amines, such as isopropylamine, diethylamine, ethanolamine, trimethylamine, dicyclohexyl amine and N-methyl-D-glucamine.
• solvates refers to a complex of variable stoichiometry formed by a solute (in this invention, a compound of Formula (I), or a salt thereof) and a solvent.
• solvents for the purpose of the invention may not interfere with the biological activity of the solute.
• suitable solvents include water, methanol, ethanol and acetic acid.
• the solvent used is a pharmaceutically acceptable solvent.
• suitable pharmaceutically acceptable solvents include water, ethanol and acetic acid.
• the term "prodrug” means a compound which is converted within the body, e.g. by hydrolysis in the blood, into its active form that has medical effects.
• Pharmaceutically acceptable prodrugs are described in T. Higuchi and V. Stella, Prodrugs as Novel Delivery Systems, Vol. 14 of the A.C.S. Symposium Series; Edward B. Roche, ed., Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press, 1987; and in D. Fleisher, S. Ramon and H. Barbra "Improved oral drug delivery: solubility limitations overcome by the use of prodrugs", Advanced Drug Delivery Reviews (1996) 19(2) 115-130, each of which are incorporated herein by reference.
• Prodrugs are any covalently bonded carriers that release a compound of formula (I) in vivo when such prodrug is administered to a patient.
• Prodrugs are generally prepared by modifying functional groups in a way such that the modification is cleaved, either by routine manipulation or in vivo, yielding the parent compound.
• Prodrugs include, for example, compounds of this invention wherein hydroxy or amine groups are bonded to any group that, when administered to a patient, cleaves to form the hydroxy or amine groups.
• representative examples of prodrugs include (but are not limited to) acetate, formate and benzoate derivatives of alcohol and amine functional groups of the compounds of formula (I). Further, in the case of a carboxylic acid (-
• esters may be employed, such as methyl esters, ethyl esters, and the like. Esters may be active in their own right and /or be hydrolysable under in vivo conditions in the human body. Suitable pharmaceutically acceptable in vivo hydrolysable ester groups include those which break down readily in the human body to leave the parent acid or its salt.
• halogen such as fluorine, chlorine, bromine or iodine
• hydroxy such as methoxy or ethoxy
• halosubstituted Ci-IO alkoxy S(0)m alkyl, such as methyl thio, methylsulfmyl or methyl sulfonyl; a ketone (-C(O)), or an aldehyde (-C(O)Rg'), such as C(O)Ci-IQ alkyl or C(O)aryl
• Rg' is hydrogen, Ci-IO alkyl, C3-7 cycloalkyl, heterocyclyl, heterocyclyl Ci-I O a lkyl, aryl, arylC 1.10 alkyl, heteroaryl or heteroarylC 1.10 alkyl (and wherein the Rg' moieties
• Ci-IO alkyl such CF2CF2H, or CF3
• an optionally substituted aryl such as phenyl, or an optionally substituted arylalkyl, such as benzyl or phenethyl, wherein these aryl containing moieties may also be substituted one to two times by halogen; hydroxy; hydroxy substituted alkyl; C 1-4 alkoxy; S(O) m C 1,4 alkyl; amino, mono & di-substituted C 1.4 alkyl amino; C 1.4 alkyl, or
• Ci_ioalkyl or “alkyl” or “alkyl i_io” is used herein to mean both straight and branched hydrocarbon chain containing the specified number of carbon atoms, e.g. Ci_ioalkyl means a straight of branched alkyl chain of at least 1, and at most 10, carbon atoms, unless the chain length is otherwise limited.
• alkyl as used herein include, but are not limited to, methyl, ethyl, n-propyl, n-butyl, n-pentyl, isobutyl, isopropyl, sec -butyl, tert- butyl or t-butyl and hexyl and the like.
• alkenyl refers to straight or branched hydrocarbon chains containing the specified number of carbon atoms and containing at least one double bond.
• C2_6alkenyl means a straight or branched alkenyl containing at least 2, and at most 6, carbon atoms and containing at least one double bond.
• alkenyl as used herein include, but are not limited to ethenyl, 2-propenyl, 3-butenyl, 2-butenyl, 2-pentenyl, 3-pentenyl, 3- methyl-2-butenyl, 3-methylbut-2-enyl, 3-hexenyl, l,l-dimethylbut-2-enyl and the like.
• alkoxy refers to straight or branched chain alkoxy groups containing the specified number of carbon atoms.
• Ci. ⁇ alkoxy means a straight or branched alkoxy containing at least 1, and at most 6, carbon atoms.
• alkoxy as used herein include, but are not limited to, methoxy, ethoxy, propoxy, prop-2-oxy, butoxy, but-2-oxy, 2- methylprop- 1 -oxy, 2-methylprop-2-oxy, pentoxy and hexyloxy.
• cycloalkyl refers to cyclic radicals, such as a non-aromatic hydrocarbon ring containing a specified number of carbon atoms.
• C3_7cycloalkyl means a non-aromatic ring containing at least three, and at most seven, ring carbon atoms.
• Representative examples of "cycloalkyl” as used herein include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl and the like.
• cycloalkenyl is used herein to mean cyclic radicals, such as a non-aromatic hydrocarbon ring containing a specified number of carbon atoms preferably of 5 to 7 carbons, which have at least one bond including but not limited to cyclopentenyl, cyclohexenyl, and the like.
• alkenyl is used herein at all occurrences to mean straight or branched chain radical of 2- 10 carbon atoms, unless the chain length is limited thereto, including, but not limited to ethenyl, 1-propenyl, 2-propenyl, 2 -methyl- 1 -propenyl, 1-butenyl, 2-butenyl and the like.
• aryl is used herein to mean phenyl, naphthyl, and indene.
• heteroaryl ring refers herein to mean a monocyclic five- to seven- membered unsaturated hydrocarbon ring containing at least one heteroatom selected from oxygen, nitrogen and sulfur.
• heteroaryl rings include, but are not limited to, furyl, pyranyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, isoxazolyl, isothiazolyl, imidazolyl, pyrazolyl, oxadiazolyl, oxathiadiazolyl, triazolyl, tetrazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, and uracil.
• heteroaryl ring refers to fused aromatic rings comprising at least one heteroatom selected from oxygen, nitrogen and sulfur.
• Each of the fused rings may contain five or six ring atoms.
• fused aromatic rings include, but are not limited to, indolyl, isoindolyl, indazolyl, indolizinyl, azaindolyl, benzoxazolyl, benzimidazolyl, benzothiazolyl, benzofuranyl, benzothiophenyl, quinolyl, isoquinolyl, quinazolinyl, quinoxalinyl, naphthyridinyl, cinnolinyl, purinyl, and phthalazinyl.
• heterocyclic rings is used herein to mean a monocyclic three- to seven-membered saturated or non-aromatic, unsaturated hydrocarbon ring containing at least one heteroatom selected from nitrogen, oxygen, sulphur or oxidized sulphur moieties, such as S(0)m, and m is 0 or an integer having a value of 1 or 2.
• heterocyclic rings shall also refer to fused rings, saturated or partially unsaturated, and wherein one of the rings may be aromatic, or heteroaromatic.
• Each of the fused rings may have from four to seven ring atoms.
• heterocyclyl groups include, but are not limited to, the saturated or partially saturated versions of the heteroaryl moieties as defined above, such as tetrahydropyrrole, tetrahydropyran, tetrahydrofuran, tetrahydrothiophene (including oxidized versions of the sulfur moiety), azepine, diazepine, aziridinyl, pyrrolinyl, pyrrolidinyl, 2-oxo- 1 -pyrrolidinyl, 3-oxo-l-pyrrolidinyl, 1,3- benzdioxol-5-yl, imidazolinyl, imidazolidinyl, indolinyl, pyrazolinyl, pyrazolidinyl, piperidinyl, piperazinyl, morpholino and thiomorpholino (including oxidized versions of the sulfur moiety).
• arylalkyl or “heteroarylalkyl”, “heterocyclicalkyl” or “cycloalkylalkyl” as used herein means a C I -4 alkyl (as defined above) attached to an aryl, heteroaryl, heterocyclic or cycloalkyl moiety (as also defined above) unless otherwise indicated.
• sulfinyl is used herein to mean the oxide S(O) of the corresponding sulfide, the term “thio” refers to the sulfide, and the term “sulfonyl” refers to the fully oxidized S (0)2 moiety.
• aroyl is used herein to mean C(O)Ar, wherein Ar is as phenyl, naphthyl, or aryl alkyl derivative such as defined above, such group include but are not limited to benzyl and phenethyl.
• alkanoyl is used herein to mean C(O)Ci-IO alkyl wherein the alkyl is as defined above.
• the term "optionally” means that the subsequently described event(s) may or may not occur, and includes both event(s) which occur and events that do not occur.
• substituted refers to substitution with the named substituent or substituents, multiple degrees of substitution being allowed unless otherwise stated.
• the compounds of the Formulas herein may have one or more asymmetric carbon atom and may occur as racemates, racemic mixtures and as individual enantiomers or diastereomers. All such isomeric forms are included within the present invention, including mixtures thereof.
• Cis (E) and trans (Z) isomerism may also occur.
• the present invention includes the individual stereoisomers of the compound of the invention and where appropriate, the individual tautomeric forms thereof, together with mixtures thereof.
• Separation of diastereoisomers or cis and trans isomers may be achieved by conventional techniques, e.g. by fractional crystallisation, chromatography or H.P.L.C.
• a stereoisomeric mixture of the agent may also be prepared from a corresponding optically pure intermediate or by resolution, such as H.P.L.C. of the corresponding racemate using a suitable chiral support or by fractional crystallisation of the diastereoisomeric salts formed by reaction of the corresponding racemate with a suitable optically active acid or base, as appropriate.
• crystalline forms of the compounds of the Formulas herein may exist as polymorphs, which are included in the present invention.
• Exemplified compounds of the compounds of this invention include the racemates, or optically active forms of the compounds of the working examples herein, and pharmaceutically acceptable salts thereof.
• the compounds of this invention may be made by a variety of methods, including standard chemistry. Any previously defined variable will continue to have the previously defined meaning unless otherwise indicated. Illustrative general synthetic methods are set out below and then specific compounds of the invention are prepared in the working Examples.
• a compound of formula (I) or a pharmaceutically acceptable salt thereof in therapy it will normally be formulated into a pharmaceutical composition in accordance with standard pharmaceutical practice.
• This invention also relates to a pharmaceutical composition comprising an effective amount of a compound of Formula (I) and a pharmaceutically acceptable carrier or diluent.
• Compounds of formula (I) and pharmaceutically acceptable salts thereof and pharmaceutical compositions incorporating such may conveniently be administered by any of the routes conventionally used for drug administration, for instance, orally, topically, parenterally or by inhalation.
• the compounds of formula (I) may be administered in conventional dosage forms prepared by combining a compound of formula (I) with standard pharmaceutical carriers according to conventional procedures.
• the compounds of formula (I) may also be administered in conventional dosages in combination with a known, second therapeutically active compound. These procedures may involve mixing, granulating and compressing or dissolving the ingredients as appropriate to the desired preparation. It will be appreciated that the form and character of the pharmaceutically acceptable character or diluent is dictated by the amount of active ingredient with which it is to be combined, the route of administration and other well-known variables.
• the carrier(s) must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
• the pharmaceutical carrier employed may be, for example, either a solid or liquid.
• solid carriers are lactose, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, stearic acid and the like.
• liquid carriers are syrup, peanut oil, olive oil, water and the like.
• the carrier or diluent may include time delay material well known to the art, such as glyceryl mono-stearate or glyceryl distearate alone or with a wax.
• the preparation can be tableted, placed in a hard gelatin capsule in powder or pellet form or in the form of a troche or lozenge.
• the amount of solid carrier will vary widely but preferably will be from about 25mg. to about Ig.
• the preparation will be in the form of a syrup, emulsion, soft gelatin capsule, sterile injectable liquid such as an ampule or nonaqueous liquid suspension.
• Compounds of Formula (I) may be administered topically, that is by non-systemic administration. This includes the application of a compound of Formula (I) externally to the epidermis or the buccal cavity and the instillation of such a compound into the ear, eye and nose, such that the compound does not significantly enter the blood stream.
• systemic administration refers to oral, intravenous, intraperitoneal and intramuscular administration.
• Drops according to the present invention may comprise sterile aqueous or oily solutions or suspensions and may be prepared by dissolving the active ingredient in a suitable aqueous solution of a bactericidal and/or fungicidal agent and/or any other suitable preservative, and preferably including a surface active agent.
• the resulting solution may then be clarified by filtration, transferred to a suitable container which is then sealed and sterilized by autoclaving or maintaining at 98-100 0 C. for half an hour.
• the solution may be sterilized by filtration and transferred to the container by an aseptic technique.
• bactericidal and fungicidal agents suitable for inclusion in the drops are phenylmercuric nitrate or acetate (0.002%), benzalkonium chloride (0.01%) and chlorhexidine acetate (0.01%).
• Suitable solvents for the preparation of an oily solution include glycerol, diluted alcohol and propylene glycol.
• Compounds of Formula (I) may be administered parenterally, that is by intravenous, intramuscular, subcutaneous intranasal, intrarectal, intravaginal or intraperitoneal administration.
• the subcutaneous and intramuscular forms of parenteral administration are generally preferred.
• Appropriate dosage forms for such administration may be prepared by conventional techniques.
• Compounds of Formula (I) may also be administered by inhalation, that is by intranasal and oral inhalation administration.
• Appropriate dosage forms for such administration, such as an aerosol formulation or a metered dose inhaler may be prepared by conventional techniques.
• the agents of the present invention are delivered via oral inhalation or intranasal administration.
• Appropriate dosage forms for such administration, such as an aerosol formulation or a metered dose inhaler may be prepared by conventional techniques.
• the compounds may be delivered in the form of an aerosol spray presentation from pressurized packs or a nebulizer, with the use of a suitable propellant, e.g. dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, a hydrofluoroalkane such as tetrafluoroethane or heptafluoropropane, carbon dioxide or other suitable gas.
• a suitable propellant e.g. dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, a hydrofluoroalkane such as tetrafluoroethane or heptafluoropropane, carbon dioxide or other suitable gas.
• a suitable propellant e.g. dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, a
• Dry powder compositions for topical delivery to the lung by inhalation may, for example, be presented in capsules and cartridges of for example gelatine or blisters of for example laminated aluminum foil, for use in an inhaler or insufflator.
• Powder blend formulations generally contain a powder mix for inhalation of the compound of the invention and a suitable powder base
• each capsule or cartridge may generally contain between 20 ⁇ g-10mg of the compound of formula (I) optionally in combination with another therapeutically active ingredient.
• the compound of the invention may be presented without excipients.
• the packing/medicament dispenser is of a type selected from the group consisting of a reservoir dry powder inhaler (RDPI), a multi-dose dry powder inhaler (MDPI), and a metered dose inhaler (MDI).
• RDPI reservoir dry powder inhaler
• MDPI multi-dose dry powder inhaler
• MDI metered dose inhaler
• reservoir dry powder inhaler By reservoir dry powder inhaler (RDPI) it is meant an inhaler having a reservoir form pack suitable for comprising multiple (un-metered doses) of medicament in dry powder form and including means for metering medicament dose from the reservoir to a delivery position.
• the metering means may for example comprise a metering cup, which is movable from a first position where the cup may be filled with medicament from the reservoir to a second position where the metered medicament dose is made available to the patient for inhalation.
• multi-dose dry powder inhaler is meant an inhaler suitable for dispensing medicament in dry powder form, wherein the medicament is comprised within a multi-dose pack containing (or otherwise carrying) multiple, define doses (or parts thereof) of medicament.
• the carrier has a blister pack form, but it could also, for example, comprise a capsule-based pack form or a carrier onto which medicament has been applied by any suitable process including printing, painting and vacuum occlusion.
• the formulation can be pre -metered (e.g. as in Diskus, see GB 2242134, US Patent Nos. 6,632,666, 5,860,419, 5,873,360 and 5,590,645 or Diskhaler, see GB 2178965, 2129691 and 2169265, US Patent No.s 4,778,054, 4,811,731, 5,035,237, the disclosures of which are hereby incorporated by reference) or metered in use (e.g. as in Turbuhaler, see EP 69715 or in the devices described in US Patents No. 6,321,747 the disclosures of which are hereby incorporated by reference).
• An example of a unit-dose device is Rotahaler (see GB 2064336 and US Patent No. 4,353,656, the disclosures of which are hereby incorporated by reference).
• the Diskus inhalation device comprises an elongate strip formed from a base sheet having a plurality of recesses spaced along its length and a lid sheet hermetically but peelably sealed thereto to define a plurality of containers, each container having therein an inhalable formulation containing a compound of Formula (I) preferably combined with lactose.
• the strip is sufficiently flexible to be wound into a roll.
• the lid sheet and base sheet will preferably have leading end portions which are not sealed to one another and at least one of the said leading end portions is constructed to be attached to a winding means.
• the hermetic seal between the base and lid sheets extends over their whole width.
• the lid sheet may preferably be peeled from the base sheet in a longitudinal direction from a first end of the said base sheet.
• the multi-dose pack is a blister pack comprising multiple blisters for containment of medicament in dry powder form. The blisters are typically arranged in regular fashion for ease of release of medicament there from.
• the multi-dose blister pack comprises plural blisters arranged in generally circular fashion on a disc-form blister pack.
• the multi-dose blister pack is elongate in form, for example comprising a strip or a tape.
• the multi-dose blister pack is defined between two members peelably secured to one another.
• US Patent No.'s 5,860,419; 5,873,360 and 5,590,645 describe medicament packs of this general type.
• the device is usually provided with an opening station comprising peeling means for peeling the members apart to access each medicament dose.
• the device is adapted for use where the peelable members are elongate sheets which define a plurality of medicament containers spaced along the length thereof, the device being provided with indexing means for indexing each container in turn. More preferably, the device is adapted for use where one of the sheets is a base sheet having a plurality of pockets therein, and the other of the sheets is a lid sheet, each pocket and the adjacent part of the lid sheet defining a respective one of the containers, the device comprising driving means for pulling the lid sheet and base sheet apart at the opening station.
• metered dose inhaler it is meant a medicament dispenser suitable for dispensing medicament in aerosol form, wherein the medicament is comprised in an aerosol container suitable for containing a propellant-based aerosol medicament formulation.
• the aerosol container is typically provided with a metering valve, for example a slide valve, for release of the aerosol form medicament formulation to the patient.
• the aerosol container is generally designed to deliver a predetermined dose of medicament upon each actuation by means of the valve, which can be opened either by depressing the valve while the container is held stationary or by depressing the container while the valve is held stationary.
• the valve typically comprises a valve body having an inlet port through which a medicament aerosol formulation may enter said valve body, an outlet port through which the aerosol may exit the valve body and an open/close mechanism by means of which flow through said outlet port is controllable.
• the valve may be a slide valve wherein the open/close mechanism comprises a sealing ring and receivable by the sealing ring a valve stem having a dispensing passage, the valve stem being slidably movable within the ring from a valve-closed to a valve-open position in which the interior of the valve body is in communication with the exterior of the valve body via the dispensing passage.
• the valve is a metering valve.
• the metering volumes are typically from 10 to
• the valve body defines a metering chamber for metering an amount of medicament formulation and an open/close mechanism by means of which the flow through the inlet port to the metering chamber is controllable.
• the valve body has a sampling chamber in communication with the metering chamber via a second inlet port, said inlet port being controllable by means of an open/close mechanism thereby regulating the flow of medicament formulation into the metering chamber.
• the valve may also comprise a 'free flow aerosol valve' having a chamber and a valve stem extending into the chamber and movable relative to the chamber between dispensing and non- dispensing positions.
• the valve stem has a configuration and the chamber has an internal configuration such that a metered volume is defined there between and such that during movement between is non-dispensing and dispensing positions the valve stem sequentially: (i) allows free flow of aerosol formulation into the chamber, (ii) defines a closed metered volume for pressurized aerosol formulation between the external surface of the valve stem and internal surface of the chamber, and (iii) moves with the closed metered volume within the chamber without decreasing the volume of the closed metered volume until the metered volume communicates with an outlet passage thereby allowing dispensing of the metered volume of pressurized aerosol formulation.
• a valve of this type is described in U.S. Patent No. 5,772,085. Additionally, intra-nasal delivery of the present compounds is effective.
• the medicament To formulate an effective pharmaceutical nasal composition, the medicament must be delivered readily to all portions of the nasal cavities (the target tissues) where it performs its pharmacological function. Additionally, the medicament should remain in contact with the target tissues for relatively long periods of time. The longer the medicament remains in contact with the target tissues, the medicament must be capable of resisting those forces in the nasal passages that function to remove particles from the nose. Such forces, referred to as 'mucociliary clearance', are recognized as being extremely effective in removing particles from the nose in a rapid manner, for example, within 10-30 minutes from the time the particles enter the nose.
• a nasal composition that it must not contain ingredients which cause the user discomfort, that it has satisfactory stability and shelf-life properties, and that it does not include constituents that are considered to be detrimental to the environment, for example ozone depletors.
• a suitable dosing regime for the formulation of the present invention when administered to the nose would be for the patient to inhale deeply subsequent to the nasal cavity being cleared. During inhalation the formulation would be applied to one nostril while the other is manually compressed. This procedure would then be repeated for the other nostril.
• the means for applying a formulation of the present invention to the nasal passages is by use of a pre-compression pump.
• the pre-compression pump will be a VP7 model manufactured by Valois SA. Such a pump is beneficial as it will ensure that the formulation is not released until a sufficient force has been applied, otherwise smaller doses may be applied.
• Another advantage of the pre-compression pump is that atomisation of the spray is ensured as it will not release the formulation until the threshold pressure for effectively atomising the spray has been achieved.
• the VP7 model may be used with a bottle capable of holding 10-50ml of a formulation. Each spray will typically deliver 50-100 ⁇ l of such a formulation, therefore, the VP7 model is capable of providing at least 100 metered doses.
• Spray compositions for topical delivery to the lung by inhalation may for example be formulated as aqueous solutions or suspensions or as aerosols delivered from pressurised packs, such as a metered dose inhaler, with the use of a suitable liquefied propellant.
• Aerosol compositions suitable for inhalation can be either a suspension or a solution and generally contain the compound of Formula (I) optionally in combination with another therapeutically active ingredient and a suitable propellant such as a fluorocarbon or hydrogen-containing chlorofluorocarbon or mixtures thereof, particularly hydrofluoroalkanes, e.g.
• the aerosol composition may be excipient free or may optionally contain additional formulation excipients well known in the art such as surfactants, e.g., oleic acid or lecithin and cosolvents, e.g. ethanol.
• Pressurized formulations will generally be retained in a canister (e.g. an aluminum canister) closed with a valve (e.g. a metering valve) and fitted into an actuator provided with a mouthpiece.
• Medicaments for administration by inhalation desirably have a controlled particle size.
• the optimum particle size for inhalation into the bronchial system is usually l-10 ⁇ m, preferably 2- 5 ⁇ m. Particles having a size above 20 ⁇ m are generally too large when inhaled to reach the small airways.
• the particles of the active ingredient as produced may be size reduced by conventional means e.g., by micronization.
• the desired fraction may be separated out by air classification or sieving.
• the particles will be crystalline in form.
• an excipient such as lactose is employed, generally, the particle size of the excipient will be much greater than the inhaled medicament within the present invention.
• the excipient is lactose it will typically be present as milled lactose, wherein not more than 85% of lactose particles will have a MMD of 60-90 ⁇ m and not less than 15% will have a MMD of less than 15 ⁇ m.
• Intranasal sprays may be formulated with aqueous or non-aqueous vehicles with the addition of agents such as thickening agents, buffer salts or acid or alkali to adjust the pH, isotonicity adjusting agents or anti-oxidants.
• agents such as thickening agents, buffer salts or acid or alkali to adjust the pH, isotonicity adjusting agents or anti-oxidants.
• Solutions for inhalation by nebulization may be formulated with an aqueous vehicle with the addition of agents such as acid or alkali, buffer salts, isotonicity adjusting agents or antimicrobials. They may be sterilised by filtration or heating in an autoclave, or presented as a non-sterile product.
• the daily topical dosage regimen will preferably be from 0.01 mg to 1000 mg, administered one to four times daily.
• the daily inhalation dosage regimen will preferably be from about 0.05 microgram/kg to about 1 mg/kg per day, more preferably from about 0.2 microgram/kg to about 20 microgram/kg, administered in one or more daily doses.
• the daily intranasal dosage regimen will preferably be from about 0.05 microgram/kg to about 1 mg/kg per day, more preferably from about 0.2 microgram/kg to about 20 microgram/kg, administered in one or more daily doses.
• the optimal quantity and spacing of individual dosages of a compound of Formula (I) or a pharmaceutically acceptable salt thereof will be determined by the nature and extent of the condition being treated, the form, route and site of administration, and the particular patient being treated, and that such optimums can be determined by conventional techniques. It will also be appreciated by one of skill in the art that the optimal course of treatment, i.e., the number of doses of a compound of Formula (I) or a pharmaceutically acceptable salt thereof given per day for a defined number of days, can be ascertained by those skilled in the art using conventional course of treatment determination tests.
• novel compounds of Formula (I) may also be used in association with the veterinary treatment of mammals, other than humans, in need of antagonism of a muscarinic receptor or a PDE-IV enzyme.
• the treatment, therapeutically or prophylactically, in animals include disease states such as those noted herein in the Methods of Treatment section.
• treatment may include prophylaxis. It may also include reducing the symptoms of, ameliorating the symptoms of, reducing the severity of, reducing the incidence of, or any other change in the condition of the patient, which improves the therapeutic outcome.
• formulations of this invention may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavoring agents, or those for inhalation may include carriers, such as lactose.
• the anticipated therapeutic activity for a dual pharmacophore antagonist of muscarinic receptors and an inhibitor of the PDE4 enzyme within a single molecule is both as a bronchodilator (provided by both muscarinic receptor antagonist activity and PDE4 inhibition) and as an antiinflammatory (by elevation of cytosolic levels of 3', 5' -cyclic adenosine monophosphate (cAMP) through inhibition of the PDE4 enzyme and by blockade other pro-inflammatory mechanisms mediated through muscarinic receptors on immune and resident cells) within the lungs.
• cAMP 3', 5' -cyclic adenosine monophosphate
• Muscarinic receptors are coupled to G-proteins (Mi, M 3 & M 5 via G q / ⁇ and M 2 & M 4 via Gj/o) which can lead to activation of a number of intracellular targets and signaling cascades.
• M 2 and M 4 receptors via d/o can decrease cellular adenylyl cyclase levels and increase MAP kinase activation
• M 1 , M 3 & M 5 receptors via G q/ n can elevate phospholipase C ⁇ (PLC ⁇ ) and increase MAP kinase activation (Nathanson NM. A multiplicity of muscarinic mechanisms: enough signaling pathways to take your breathe away. Proc. Natl. Acad. ScL USA. 2000; 97:6245-6247.
• Lanzafame AA Cellular signaling mechanisms for muscarinic acetylcholine receptors. Recept. Chann. 2003; 9:241-260).
• Elevated levels of cyclic AMP has been shown to have anti-inflammatory activity in a range of immune cells including T-cells, macrophages and neutrophils as well as resident lung cells such as epithelial and airway smooth muscle cells. Elevated cAMP can also cause airway smooth muscle relaxation and may offer a further mechanism independent of M 3 receptor blockade to initiate bronchodilation.
• PDE4 inhibitors see: Kroegel C & Foerster M. Phophodiesterase-4 inhibitors as a novel approach for the treatment of respiratory disease: cilomilast. Expert Opin. Investig. Drugs 2007; 16: 109-124. Dastidar SG.
• the disposition within the lungs of a single drug substance which acts at both muscarinic receptors and as a PDE4 inhibitor at the same cell offers the greatest opportunity for cooperative anti-inflammatory or bronchodilator activity through modulation of these independent targets.
• This approach offers a greater potential to maximize the interaction of these two independent mechanisms compared to co-administration of two pharmacophores directed against each target as co-disposition at the cells of the lungs cannot be guaranteed through the second approach.
• the novel single dual pharmacophore approach outlined here therefore offers a significantly greater potential for co-disposition to cells of the lung compared to administration of two separate pharmacophores directed against each target. Further to this such a pharmacophore will also be more amenable to combination with existing or other novel inhaled therapies for the treatment of respiratory diseases.
• compounds and pharmaceutical formulations according to the invention may be used in combination with or include one or more other therapeutic agents, for example selected from anti-inflammatory agents, other selective anticholinergic agents (particularly an M 1 , M 2 , or Mi/M 2 receptor antagonist), ⁇ 2 -adrenoreceptor agonists, antiinfective agents (e.g. antibiotics, antivirals), or antihistamines.
• other therapeutic agents for example selected from anti-inflammatory agents, other selective anticholinergic agents (particularly an M 1 , M 2 , or Mi/M 2 receptor antagonist), ⁇ 2 -adrenoreceptor agonists, antiinfective agents (e.g. antibiotics, antivirals), or antihistamines.
• the invention thus provides, in a further aspect, a combination comprising a compound of formula (I) or a pharmaceutically acceptable salt, solvate or physiologically functional derivative thereof together with one or more other therapeutically active agents, for example selected from an anti-inflammatory agent (for example a corticosteroid or an NSAID), an anticholinergic agent, ⁇ 2 -adrenoreceptor agonist, an antiinfective agent (e.g. an antibiotic or an antiviral), or an antihistamine.
• an anti-inflammatory agent for example a corticosteroid or an NSAID
• an anticholinergic agent for example a corticosteroid or an NSAID
• an antiinfective agent e.g. an antibiotic or an antiviral
• PDE-4 inhibitor e.g. an antibiotic or an antiviral
• Preferred combinations are those comprising one or two other therapeutic agents.
• the other therapeutic ingredient(s) may be used in the form of salts, (e.g. as alkali metal or amine salts or as acid addition salts), or prodrugs, or as esters (e.g. lower alkyl esters), or as solvates (e.g. hydrates) to optimize the activity and/or stability and/or physical characteristics (e.g. solubility) of the therapeutic ingredient.
• the therapeutic ingredients may be used in optically pure form.
• One suitable combination of the present invention comprises of compound of the invention together with a ⁇ 2 -adrenoreceptor agonist.
• ⁇ -adrenoreceptor agonists include salmeterol (which may be a racemate or a single enantiomer, such as the R-enantiomer), salbutamol, formoterol, salmefamol, fenoterol or terbutaline and salts thereof, for example the xinafoate salt of salmeterol, the sulphate salt or free base of salbutamol or the fumarate salt of formoterol.
• Long-acting ⁇ -adrenoreceptor agonists are preferred, especially those having a therapeutic effect over a 24 hour period, such as salmeterol or formoterol.
• Suitable long acting ⁇ -adrenoreceptor agonists include those described in WO02/66422A,
• WO02/270490 WO02/076933, WO03/024439, WO03/072539, WO 03/091204, WO04/016578, WO04/022547, WO04/037807, WO04/037773, WO04/037768, WO04/039762, WO04/039766, WOO 1/42193 and WO03/042160, whose disclosures are incorporated by reference herein.
• Preferred long-acting ⁇ 2 -adrenoreceptor agonists are: 3-(4- ⁇ [6-( ⁇ (2R)-2-hydroxy-2-[4-hydroxy-3-(hydroxymethyl)phenyl]ethyl ⁇ amino) hexyl] oxy ⁇ butyl)benzenesulfonamide; 3-(3- ⁇ [7-( ⁇ (2R)-2-hydroxy-2-[4-hydroxy-3-hydroxymethyl)phenyl]ethyl ⁇ - amino)heptyl] oxy ⁇ propyl)benzenesulfonamide;
• Suitable anti-inflammatory agents include corticosteroids.
• Suitable corticosteroids which may be used in combination with the compounds of the invention are those oral and inhaled corticosteroids and their pro-drugs which have anti- inflammatory activity. Examples include methyl prednisolone, prednisolone, dexamethasone, fluticasone propionate, 6 ⁇ ,9 ⁇ -difluoro-17 ⁇ -
• Preferred corticosteroids include fluticasone propionate, 6 ⁇ ,9 ⁇ -difluoro-l 1 ⁇ -hydroxy- 16 ⁇ -methyl-l 7 ⁇ -[(4-methyl- 1,3 - thiazole-5-carbonyl)oxy]-3-oxo-androsta-l,4-diene-17 ⁇ -carbothioic acid S-fluoromethyl ester and 6 ⁇ ,9 ⁇ -difluoro- 17 ⁇ -[(2-furanylcarbonyl)oxy]- 11 ⁇ -hydroxy- 16 ⁇ -methyl-3-oxo-androsta- 1 ,4-diene- 17 ⁇ -carbothioic acid S-fluoromethyl ester, more preferably 6 ⁇ ,9 ⁇ -difluoro-17 ⁇ -[(2- furanylcarbonyl)oxy]-l l ⁇ -hydroxy-16 ⁇ -methyl-3-oxo-androsta-l,4-diene-17 ⁇ -carbothioic acid S- fluoromethyl ester.
• Non-steroidal compounds having glucocorticoid agonism that may possess selectivity for transrepression over transactivation and that may be useful in combination therapy include those covered in the following patents: WO03/082827, WO01/10143, WO98/54159, WO04/005229, WO04/009016, WO04/009017, WO04/018429, WO03/104195, WO03/082787, WO03/082280, WO03/059899, WO03/101932, WO02/02565, WO01/16128, WO00/66590, WO03/086294, WO04/026248, WO03/061651, WO03/08277.
• Suitable anti-inflammatory agents include non-steroidal anti-inflammatory drugs (NSAID 's).
• NSAID 's include sodium cromoglycate, nedocromil sodium, leukotriene antagonists, inhibitors of leukotriene synthesis (for example, montelukast), iNOS inhibitors, tryptase and elastase inhibitors, beta-2 integrin antagonists and adenosine receptor agonists or antagonists (for example, adenosine 2a agonists), cytokine antagonists (for example, chemokine antagonists, such as a CCR3 antagonist) or inhibitors of cytokine synthesis, 5-lipoxygenase inhibitors, p38 inhibitors, and IKK2 inhibitors.
• chemokine antagonists for example, chemokine antagonists, such as a CCR3 antagonist
• Suitable other ⁇ 2 -adrenoreceptor agonists include salmeterol (for example, as the xinafoate), salbutamol (for example, as the sulphate or the free base), formoterol (for example, as the fumarate), fenoterol or terbutaline and salts thereof.
• An iNOS (inducible nitric oxide synthase inhibitor) is preferably for oral administration.
• Suitable iNOS inhibitors include those disclosed in WO93/13055, WO98/30537, WO02/50021, WO95/34534 and WO99/62875.
• Suitable CCR3 inhibitors include those disclosed in WO02/26722.
• Suitable antihistamines include any one or more of the numerous antagonists known which inhibit Hi-receptors, and are safe for human use. All are reversible, competitive inhibitors of the interaction of histamine with Hi-receptors. The majority of these inhibitors, mostly first generation antagonists, are generally represented by three types of antihistamines: ethanolamines, ethylenediamines, and alkylamines. In addition, other first generation antihistamines include those which can be characterized as based on piperizine and phenothiazines.
• Second generation antagonists which are non-sedating, have a similar structure- activity relationship in that they retain the core ethylene group (the alkylamines) or mimic the tertiary amine group with piperizine or piperidine.
• Exemplary antagonists are as follows: Ethanolamines: carbinoxamine maleate, clemastine fumarate, diphenylhydramine hydrochloride, and dimenhydrinate.
• Ethylenediamines pyrilamine maleate, tripelennamine HCl, and tripelennamine citrate.
• Alkylamines chloropheniramine and its salts such as the maleate salt, and acrivastine.
• Piperazines hydroxyzine HCl, hydroxyzine pamoate, cyclizine HCl, cyclizine lactate, meclizine HCl, and cetirizine HCl.
• Piperidines Astemizole, levocabastine HCl, loratadine or its descarboethoxy analogue, and terfenadine and fexofenadine hydrochloride or another pharmaceutically acceptable salt.
• compositions comprising a combination as defined above together with a physiologically acceptable diluent or carrier represent a further aspect of the invention.
• the individual compounds of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations. Appropriate doses of known therapeutic agents will be readily appreciated by those skilled in the art.
• Desired attributes of the molecule would be to maintain or improve the M 3 pharmacophores potency with no or partial Mi agonism since agonism of the Mi receptor is usually counter- indicated. Another attribute is to decrease the dropoff between the PDE4 enzyme assay and inhibition reflected in the PBMC assay. Since both pharmacophores are in a single molecule, it is desirable to enhance intracellular inhibition of PDE4 reflected in the PBMC assay while retaining significant activity against the transmembrane M 3 receptor. In addition, in vivo efficacy and duration of action is not always reflected by in vitro measurements of activity, therefore other physiochemical properties of the molecules may be important for balanced efficacy at both targets.
• one embodiment of the invention are compounds which posses appropriately balanced pharmacology, and have desirable physicochemical properties, such as solubility, dissolution rate, permeability, crystallinity, micronizability, and excipient compatibility. If the compounds are administered by inhalation, then low aqueous solubility is generally not suitable for a nebulized/solution formulation.
• One embodiment of the invention is a display of sufficient antagonism at the M 3 receptor wherein pIC50 > 8.0 and a pA 2 > 8.0, as well as inhibition of the PDE4 enzyme with a pIC 5 o ⁇ 8.0 and cellular activity (as reflected in the PBMC assay) with a pIC 5 o> 7.0.
• compounds of Formula (I) are generally selective against agonism or partial agonism of the various muscarinic receptors (Mi, M 2 , M 3 ) and PDE4 > 100-fold vs. other PDEs.
• the inhibitory effects of compounds at the mAChR (muscarinic) receptor and the PDE4 enzyme for the present invention are determined by the following in vitro and in vivo functional assays. mAChR (Muscarinic)Receptor Assays In vitro assays
• the human Mi - M3 receptors are cloned and stably expressed in Chinese Hamster Ovary (CHO) cell lines.
• M 2 ACh receptor is co-expressed with the chimeric G protein, Gqi5, in CHO cells.
• Competition for [ 3 H]-N-methyl scopolamine (0.5 nM) binding is performed using crude CHO cell membranes using a Scintillation Proximity Assay (SPA). Atropine is run in every assay as the control.
• SPA Scintillation Proximity Assay
• SPA assay membranes are preincubated with wheatgerm agglutinin beads (GE) in 50 mM HEPES buffer (Sigma, St. Louis MO) (pH 7.4) at 4° C for 30 min, and then incubated with 0.5 nM [ H]-N -methyl scopolamine (PerkinElmer) in a 96-well Optiplate (Perkin Elmer), for 2 hr in the presence of vehicle (1% DMSO) or compound (0.01-1000 nM), in 0.2 mL final volume, at room temperature. At the end of the incubation the plates are centrifuged (Beckman CS-6R) for 5 min at 2000 RPM, and counted in a Top Count Microplate Scintillation counter (model A9912 Packard, Meriden CT).
• Concentration-response curves for each compound are run using duplicate samples in 3 independent experiments. Specific binding is determined by subtracting non-specific binding (defined in the presence of 0.3 ⁇ M Atropine) from total binding. IC50 values are estimated from concentration-response curves and used to determine the inhibition constant (Ki) of each inhibitor using the Cheng and Prusoff equation [for competitive antagonists: The Kd's utilized for the calculations are: 0.17, 0.28, and 0.16, nM for Ml, M2 and M3 respectively.
• Cells are harvested by centrifugation at 1000 x g for 10 min at 4 0 C.
• the cell pellet is washed with Phosphate Buffered Saline (PBS) and quick frozen with liquid nitrogen.
• PBS Phosphate Buffered Saline
• the pellet is stored at - 80 0 C until the membrane preparation is made.
• the frozen pellet is thawed and re-suspended in cold hypotonic membrane buffer (40 mM Tris, pH 7.5, 1 mM MgSO 4 , 0.5 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 2.5 mg/L leupeptin, 0.1 mg/mL aprotinin) and incubated on ice for 5 min.
• cold hypotonic membrane buffer 40 mM Tris, pH 7.5, 1 mM MgSO 4 , 0.5 mM EDTA, 1 mM phenylmethylsulfonyl fluoride
• the cell suspension is homogenized in a 40 mL Dounce homogenizer and centrifuged at 2000 rpm at 4 0 C for 6 min to remove nuclei and cellular debris.
• the 2000 rpm pellet is resuspended in homogenization buffer and spun again at 2000 rpm for 6 min. This process is repeated two more times.
• the combined supernatant is collected and cell membranes are pelleted at 100000 x g for 1 hr at 4 0 C.
• the membrane pellet is resuspended in membrane buffer and aliquots stored at - 80 0 C. Protein concentration is quantified using the Bio-Rad protein assay reagent.
• the human M1-M3 receptors are cloned and stably expressed in Chinese Hamster Ovary (CHO) cells.
• the M2 receptors are co-expressed with the chimeric G protein, Gqi5.
• CHO-Ml , CHO-Gqi5-M2 and CHO-M3 cells are cultured to confluence at 37 0 C in a humidified incubator with 5% CO 2 /95% air.
• CHO-Ml and CHO- M3 are cultured in Alpha MEM with nucleosides and L-glutamine and 10% fetal calf serum.
• Cells expressing the M2 receptor are cultured in DMEM/F12 media, supplemented with 200 mg/L G418 (geneticin), and 10% fetal calf serum.
• Assay Readout Calcium mobilization, monitored as change in cytosolic calcium concentration, is measured as change in 516 nm emission fluorescence intensity of cytosolic loaded Fluo-4, a green fluorescent calcium indicator which exhibits large (> 100- fold) fluorescence intensity increases on binding to calcium, the change in intensity being, therefore, directly related to cytosolic calcium levels.
• the emitted fluorescence from all 96 wells is measured simultaneously using a cooled CCD camera. Data points are collected every second. Maximal change in emission from each well after simultaneous addition of agonist or compound to each of the 96 wells is then exported to an excel spreadsheet. This data is then transferred to GraphPad Prism Version 4.03 for plotting of response to each treatment condition (ACh or compound).
• M2 (w/Gqi5) and M3 ACh receptors stably expressed in CHO cells.
• cells are plated in 96 well, blackwall, clear bottom plates (Packard View) at a concentration of 40000 cells per well and incubated at 37 0 C in a humidified incubator with
• IC50 Determination for Antagonists Receptor antagonist characterization (IC 50 determination), compounds tested for potency of inhibition of ACh induced muscarinic receptor activation: To evaluate antagonist potency of compounds against the M 1 , M 2 and M 3 receptors, cell culture media is aspirated and replaced with 100 ⁇ L of dye load media [Eagles Minimal Essential Medium (EMEM) with Earl's salts and L-Glutamine, 0.1% BSA (Se ologicals Corporation), 4 ⁇ M Fluo-4- acetoxymethyl ester fluorescent indicator dye (Fluo-4 AM, Molecular Probes, Eugene, OR) and 2.5 mM probenecid]. Cells are then incubated for 1 hour at 37 0 C.
• EMEM Eagles Minimal Essential Medium
• BSA Se ologicals Corporation
• Fluo-4- acetoxymethyl ester fluorescent indicator dye Fluo-4 AM, Molecular Probes, Eugene, OR
• the dye load media is then aspirated off the cells and replaced with identical media without Fluo-4 AM and with 0.1% Gelatin (BSA removed) and 2.5 mM probenecid.
• Cells are incubated for 10 minutes at 37 0 C and then washed 3 times with KRH assay buffer [Rrebs Ringer Henseleit (120 mM NaCl, 4.6 mM KCl, 1.03 mM KH 2 PO 4 , 25 mM NaHCO 3 , 1.0 mM CaCl 2 ,
• the basal emission fluorescence is measured, then the cellular response to an ECso concentration of ACh (3.3 nM against M 1 , 10 nM against M 2 and 1.0 nM against M 3 ) prepared in KRH assay buffer with 0.1% BSA (no gelatin), is monitored in FLIPR for 90 seconds and then 50 ⁇ L of 100 ⁇ M ATP (assay concentration of 20 ⁇ M) is added to check cell viability (H. M. Sarau et a ⁇ , 1999. MoI. Pharmacol. 56, 657-663). Maximal change in emission from each well, vehicle or compound pretreated, after simultaneous addition of ACh to each of the 96 wells is then determined.
• the IC50 is defined as the compound pretreatment concentration which inhibits 50% of the ACh induced response.
• a compound is believed to be active in this assay if it has an IC 50 of between 33 uM and 10 nM or less.
• Exemplary compounds of Formula (I) which have been tested in this assay and found to be the most active can be found in
• p A 2 Determination for Antagonists Single concentration kinetic characterization of compounds tested for potency of inhibition of ACh induced muscarinic receptor activation'. pA2.' Compounds which show ICso's of ⁇ 1.0 ⁇ M may be further characterized in a single compound concentration kinetic assay.
• dye loaded (culture media is aspirated, replaced with 100 ⁇ L of dye load media and incubated for 1 hour at 37 0 C) and washed cells (washed three times with 100 ⁇ L KRH assay buffer) are treated with 150 ⁇ L of KRH assay buffer with 0.1% gelatin and 2.5 mM probenecid containing vehicle (0.01% DMSO), for control response, or appropriate concentration of antagonist (single concentration for each column of 12 wells, concentration determined from IC50 value) and incubated for 20 minutes at 37 0 C.
• Buffer is aspirated off and 150 ⁇ L of fresh KRH assay buffer with 0.1% gelatin and 2.5 mM probenecid containing vehicle (0.01% DMSO) or appropriate concentration of compound is added and incubated for 10 minutes at 37 0 C. Plates are then placed into FLIPR for fluorescent measurements. After determination of basal fluorescence emission, a concentration range of ACh (0.033-100,000 nM for M1/M3 and 0.33-1,000,000 nM for M2) is added to vehicle or compound treated (columns of 12 wells) cells to determine the shift of receptor potency in response to ACh in presence of compound.
• EMEM is aspirated and KRH assay buffer containing 0.1% gelatin with vehicle (0.01% DMSO) or antagonist is added to washout columns and incubated at 37 0 C for 20 minutes. Buffer with vehicle or compound is aspirated and cells retreated and incubated at 37 0 C for an additional 10 minutes. Buffer with vehicle or compound is then aspirated and cells washed 3 times with KRH assay buffer containing 0.1% BSA. KRH buffer (100 ⁇ L) containing 0.1% BSA is then added and cells incubated for 30 minutes at 37 0 C and washed 3 times. Cells are incubated for a further 30 minutes and washed 3 times, followed by a further 30 minute incubation.
• Dye load media is aspirated and cells are retreated with 150 ⁇ L of KRH assay buffer containing 0.1% gelatin and 2.5 mM probenicid for washout columns or KRH assay buffer containing 0.1% gelatin and 2.5 mM probenicid and vehicle or appropriate concentration of compound for no washout columns. Cells are incubated for 20 minutes at 37 0 C. Pretreatment buffer is aspirated and 150 ⁇ L of fresh
• KRH assay buffer with 0.1% gelatin and 2.5 mM probenecid is then added to washout columns and the same buffer containing vehicle (0.01% DMSO) or the appropriate concentration of antagonist is added to the no washout columns. Plates are incubated for 10 minutes at 37 0 C and plates placed into FLIPR where fluorescence is monitored. Baseline measurements are recorded and acetylcholine concentration response curves are added to each column while continuing to monitor fluorescence. Comparison of ACh concentration response curves is performed between vehicle-treated and antagonist-treated [1.0 nM, 10 nM, 10OnM or 1000 nM] cells following washout to determine if there remained a shift in the EC 50 value post washout.
• Receptor agonist characterization (EC 50 determination): compounds tested to confirm no agonist potential at muscarinic receptors: To evaluate agonist potential of compounds and ACh potency for the M 1 , M 2 and M3 receptors, culture media is aspirated and replaced with 100 ⁇ L of dye load media. Cells are then incubated for 1 hour at 37 0 C. The dye load media is then aspirated off the cells and replaced with identical media without Fluo-4 AM and with 0.1% Gelatin (BSA removed) and 2.5 mM probenecid. Cells are incubated for 10 minutes at 37 0 C and then washed 3 times with 100 ⁇ L KRH assay buffer.
• Composition of the solution was (mM): NaCl (113.0), KCl (4.8), CaCl 2 (2.5), KH 2 PO 4 (1.2), MgSO 4 (1.2), NaHCO 3 (25.0) and dextrose (11.0) and equilibrated with 95% O 2 : 5% CO 2 and maintained at 37 0 C; meclofenamic acid (1 ⁇ M) was added to block endogenous cycloxygenase activity.
• trachea was removed from male Hartely guinea pigs (Charles River, Portage, MI; weight range 450-650 g). The epithelium of the trachea was removed and strips were cut, approximately 2 cartilage rings in width.
• Protocol A Tissues were suspended under an optimal resting tension of 1.5 g.
• a carbachol concentration-response curve was then generated, whole-log increments from 10 nM to 100 ⁇ M, followed by a 1 mM histamine-induced contraction for reference.
• the onset halftime to maximal inhibition of tension (ON ty 2 ) was determined.
• the offset halftime of tension recovery (OFF tyi) following removal of the compound from the superfusate, was determined by measuring the time required for tension to return to the level used to measure the respective onset halftime. Tension recovery was plotted vs. time as a percentage of the % recovery of maximal inhibition.
• Post-recovery concentration-response curves were plotted with data as a percent of the 1 mM post-histamine reference contractions. EC 50 and fold- shift vs. control values were calculated for each compound tested.
• Protocol B Tissues were suspended under an optimal resting tension of 1.5 g. After an incubation period to reach stable basal tone, histamine (10 ⁇ M) was infused to assess tissue contraction response. After tension reached a plateau, histamine infusion was halted and tissues tension allowed to return to baseline. Compounds and vehicle were then infused onto the tissues for 6 hours. A carbachol concentration-response curve was generated, in the presence of infused compounds or vehicle, by infusing carbachol over the tissues in cumulative half-log increments, 10 nM to 100 ⁇ M, followed by a 1 ⁇ M histamine-induced contraction for reference. Upon completion of this curve, infusion of compounds into the perfusate was halted and tissue tension allowed to return to baseline.
• EC50 and fold-shift vs. control values were calculated for each compound tested.
• Procedure for wet suspension intratracheal dosing A stock solution of 5% weight/volume of Tween 80 is made at least one day prior to dosing. The solution is made by dissolving 1 gram of Tween 80 in a total volume of 20 ml sterile saline. On the day of dosing the stock 5% Tween solution is diluted 1 : 10 in sterile saline for a final concentration of 0.5% Tween. This solution is filtered through a 0.22 micron syringe filter to yield the final wet vehicle. Animals are weighed and the weights averaged for dose calculations:
• Drug is weighed and placed into a glass homogenizer with the appropriate amount of vehicle, i.e. if 1.5 mg is weighed, than 1 ml vehicle is be added. The mixture is then homogenized by hand until it appears uniform. For doses lower than 1.0 mg/kg appropriate dilution of the suspension is made immediately after homogenization. A one ml syringe capped with a 22ga 2.5 inch rat gavage needle is filled with 200 ⁇ l of dosing solution. After an animal is anesthetized with isoflorane they are placed in the supine position and the dosing needle is introduced into the trachea via the mouth. After the drug solution is injected into the trachea the animal is returned to a recovery cage. Recovery from anesthesia is noted within 5 minutes.
• Penh [(expiratory time / relaxation time)-l] x (peak expiratory flow / peak inspiratory flow) where relaxation time is the amount of time required for 70% of the tidal volume to be expired. Animals are returned to caging until the next noted exposure timepoint. Each animal's baseline airway parameter is used as its own control when determining the effect of ACh aerosol exposure.
• Human recombinant PDE4B in particular the 2B splice variant thereof (HSPDE4B2B), is disclosed in WO 94/20079 and also in M.M. McLaughlin et al, "A low Km, rolipram-sensitive, cAMP-specific phosphodiesterase from human brain: cloning and expression of cDNA, biochemical characterization of recombinant protein, and tissue distribution of mRNA", J. Biol. Chem., 1993, 268, 6470-6476.
• human recombinant PDE4B is described as being expressed in the PDE- deficient yeast Saccharomyces cerevisiae strain GL62. PDE4B expression is induced by the addition of 150 ⁇ M C11SO4.
• HSPDE4D3A Human recombinant PDE4D (HSPDE4D3A) is disclosed in P. A. Baecker et al., "Isolation of a cDNA encoding a human rolipram-sensitive cyclic AMP phosphodiesterase (PDE IV D )", Gene, 1994, 138, 253-256. Expression of human PDE4D in yeast, and subsequent preparation of the recombinant protein for assay was as described for PDE4B.
• Inhibition of PDE4B and PDE4D are measured using a luminescence-coupled assay system developed by Cambrex.
• This assay system couples the formation of AMP, derived from PDE4-catalyzyed hydrolysis of cAMP, to the formation of ATP. The ATP is then used as a substrate for Luciferase and results in light as a signal output.
• PDE is inhibited or inactive, no AMP is produced, the Luciferase is inactive, and no light signal is produced.
• This assay is used in a quenched assay format, where PDE4 enzyme (2.5 ⁇ L; ⁇ 120pM enzyme in 4OmM Tris-HCl, 1OmM MgCl 2 , ImM CHAPS, 0.01% BSA, pH 7.5.) and cAMP substrate (2.5 ⁇ L; 2 ⁇ M cAMP in 4OmM Tris-HCl, 1OmM MgCl 2 , ImM CHAPS, 0.01% BSA, pH 7.5.) are added sequentially to a 384 well assay plate (Greiner 784075) pre-stamped with 12.5-50 nL compound at the desired concentration.
• PDE4 enzyme 2.5 ⁇ L; ⁇ 120pM enzyme in 4OmM Tris-HCl, 1OmM MgCl 2 , ImM CHAPS, 0.01% BSA, pH 7.5.
• cAMP substrate 2.5 ⁇ L; 2 ⁇ M cAMP in 4OmM Tris-HCl, 1OmM MgCl 2
• reaction is incubated at room temperature for 1 hr, then is quenched by the addition of enzyme stop solution (1.5 ⁇ L ; prepared as described by vendor; catalog # LT27-253) and then the light signal is generated by the addition of detection reagent (2.5 ⁇ L, prepared as described by vendor, catalog# LT27-250).
• enzyme stop solution 1.5 ⁇ L ; prepared as described by vendor; catalog # LT27-253
• detection reagent 2.5 ⁇ L, prepared as described by vendor, catalog# LT27-250.
• the luminescence is then measured on a Viewlux imager (Perkin Elmer) using emission filters of 613/55nm or 618/40nm and a 5 second exposure.
• Compounds are prepared in neat DMSO at a concentration of 10 mM. For inhibition curves, compounds are diluted using a three fold serial dilution and tested at 11 concentrations (e.g.
• a 96-well flat bottom polystyrene tissue culture plate (manufacturer code 167008 Thermo Fisher Scientific, Kamstrupvej 90, Kamstrup, Roskilde DK-4000 Denmark ) is prepared by initially adding to column 1 ca. 1OmM of test compound dissolved in DMSO, which is diluted about 7.94 fold in the well with DMSO to give a 1.26mM solution. For a more potent compound, a more diluted solution in DMSO may be used. The compound is further diluted with DMSO into columns 2 to 9 by 8 successive 3-fold dilutions using the Biomek® 2000 Laboratory Automation Workstation (Beckman Coulter, Inc., 4300 N. Harbor Boulevard, P.O.
• PBMC cells peripheral blood mononuclear cells
• PBMC cells peripheral blood mononuclear cells
• heparinised human blood using 1% v/v Heparin Sodium 1000IU/ml Endotoxin Free, Leo Laboratories Ltd., Cashel Road, Dublin 12. Ireland, Cat No: PL0043/0149
• AccuspinTM System-Histopaque ® -1077 essentially (Sigma- Aldrich Company
• RPMI 1640 medium Low endotoxin RPMI 1640 medium, Cat No: 31870, Invitrogen Corporation Invitrogen Ltd, 3 Fountain Drive, Inchinnan Business Park, Paisley PA4 9RF, UK
• RPMI 1640 medium Low endotoxin RPMI 1640 medium, Cat No: 31870, Invitrogen Corporation Invitrogen Ltd, 3 Fountain Drive, Inchinnan Business Park, Paisley PA4 9RF, UK
• Viable cells are counted by trypan blue staining and diluted to IxIO ⁇ viable cells/ml.
• About 50 ⁇ l (about 5OuI) of diluted cells and about 75 ⁇ l (about 75ul) of LPS (ca. 1 ng/ml final; Sigma Cat No: L-6386) are added to the compound plate, which is then incubated at 37 0 C, 5% CO2, for about 20 hours.
• TNF- ⁇ concentrations of TNF- ⁇ are determined by electrochemiluminescence assay using the Meso Scale Discovery (MSD) technology (Meso Scale Discovery, 9238 Gaither Road, Gaithersburg, Maryland 20877, USA). See the "TNF- ⁇ (TNF-alpha) MSD Assay” described below for typical details.
• MSD Meso Scale Discovery
• Results can be expressed as pIC50 values for inhibition of TNF- ⁇ (TNF-alpha) production in PBMCs, and it should be appreciated that these results can be subject to variability or error.
• MSD Human Serum Cytokine Assay Diluent (25 ⁇ l) Meso Scale Discovery, 9238 Gaither Road, Gaithersburg, Maryland 20877) is added to a 96-well High-Bind MSD plate pre- coated with anti-hTNF alpha capture antibody (MA6000) and then incubated for about 24 hours at 4°C to prevent non-specific binding.
• About 20 ⁇ l (ul) of supernatant from the PBMC plate are then transferred from columns 1-11 to columns 1-11 of the MSD plate using the Biomek FX.
• About 20 ⁇ l (ul) of TNF- ⁇ standard (Cat No.
• the XC50 module automatically constrains A, B or A and B if an acceptable unconstrained fit cannot be achieved. QC criteria are applied and fits are rejected where A ⁇ -40 or >30, B ⁇ 80 or >140 or the ratio of upper and lower confidence limits on C >10. The results for each compound are recorded as pIC50 values (-C in the above equation).
• Compounds are considered active in this assay if they demonstrated a pICso of greater than 5 up to a pICso of 10 or greater, and were screened at concentrations up to 10 uM.
• Representative compounds of Formula (I) as described in Examples 111-122, 124, 127-129, 133-134, 137-141, 143-163, 165, 167-179, 182-183, 185, 187-190, and 192 were tested in the above assay and found to be the most active.
• in vitro enzymatic PDE4B inhibition assay(s) described herein, or generally similar or generally analogous assays should be regarded as being the primary test(s) of biological activity.
• additional in vivo biological tests which are not an essential measure of activity, efficacy or side-effects but may be used for further characterization are described below.
• LPS-induced pulmonary neutrophilia in rats effect of it.
• PDE4 inhibitors Pulmonary neutrophil influx is thought to be a significant component to the family of pulmonary diseases like chronic obstructive pulmonary disease (COPD) which can involve chronic bronchitis and/or emphysema (G.F. Filley, Chest. 2000; 117(5); 251s-260s).
• COPD chronic obstructive pulmonary disease
• the purpose of this neutrophilia model is to study the potentially anti-inflammatory effects in vzVo of orally administered PDE4 inhibitors on neutrophilia induced by inhalation of aerosolized lipopolysaccharide (LPS), modeling the neutrophil inflammatory component(s) of COPD. See the literature section below for scientific background.
• mice Male Lewis rats (Charles River, Raleigh, NC, USA) weighing approximately 280-400 grams are pretreated with a single intratracheal dose (200 ⁇ l) of either 300 ⁇ g/kg, or 30 ⁇ g/kg, of the test compound suspended in 0.5% Tween 80 (Sigma- Aldrich, St Louis, MO, USA) in phosphate buffered saline or vehicle only.
• dose response curves may be generated using intratracheal doses of 300, 30 and 10 ⁇ g/kg, again administered in 0.5% Tween 80 (Sigma- Aldrich, St Louis, MO, USA) in phosphate buffered saline (200 ⁇ l per rat, 30 minutes prior to LPS exposure.
• the rats are exposed to aerosolized LPS (Serotype E. CoIi 026 :B6 prepared by trichloroacetic acid extraction, Sigma- Aldrich, St Louis, MO, USA), generated from a nebulizer containing a 100 ⁇ g/mL LPS solution. Rats are exposed to the LPS aerosol at a rate of ca. 4 L/min for. 20 minutes. LPS exposure is carried out in a closed chamber with internal dimensions of roughly 45 cm length x 24 cm width x 20 cm height. The nebulizer and exposure chamber are contained in a certified fume hood.
• LPS Cerotype E. CoIi 026 :B6 prepared by trichloroacetic acid extraction, Sigma- Aldrich, St Louis, MO, USA
• BAL Bronchoalveolar lavage
• 5 ml washes are performed to collect a total of 25 ml of BAL fluid.
• Total cell counts and leukocyte differentials are performed on the BAL fluids in order to calculate neutrophil influx into the lung.
• percent inhibition of neutrophil number, neutrophil percent, or both may be calculated and reported for that specific dose.
• percent neutrophil inhibitions of either neutrophil number or neutrophil percent at each dose cf.
• a sigmoidal dose- response curve (variable slope) usually using Prism Graph-Pad.
• the dose-response curve may also be used to calculate an ED50 value (in mg per kg of body weight) for inhibition by the test compounds of the LPS-induced neutrophilia.
• Examples are listed as producing "significant” inhibition if the test compound demonstrated significant (p ⁇ 0.05, using a two tailed distribution and two sample equal variance students T test performed in Microsoft Excel) inhibition of either neutrophil number, neutrophil percent, or both, when dosed at either 300 or 30 ⁇ g/kg, 30 minutes prior to LPS aerosol exposure.
• Suitable common protecting groups for use with hydroxyl groups and nitrogen groups are well known in the art and described in many references, for instance, Protecting Groups in Organic Synthesis, Greene et al., John Wiley & Sons, New York, New York, (2nd edition, 1991 or the earlier 1981 version).
• Suitable examples of hydroxyl protecting groups include ether forming groups such as benzyl, and aryl groups such as tert-butoxycarbonyl (Boc), silyl ethers, such as t-butyldimethyl or t- butyldiphenyl, and alkyl ethers, such as methyl connected by an alkyl chain of variable link,
• (CRi()R2 ⁇ )n- Amino protecting groups may include benzyl, aryl such as acetyl and trialkylsilyl groups.
• Carboxylic acid groups are typically protected by conversion to an ester that can easily be hydrolyzed, for example, trichloethyl, tert-buiyl, benzyl and the like.
• the compounds of Formula (I) may be obtained by applying the synthetic procedures and as detailed in the working examples described herein. These synthetic schemes as provided are applicable to producing compounds of the Formulas herein having a variety of different Rj, R2, R3, Xl, Z, ArI, Ar2, and Rg, etc. groups which are reacted, employing optional substituents which may be suitably protected, to achieve compatibility with the reactions outlined herein. Subsequent deprotection, in those cases as necessary, affords compounds of the nature generally disclosed. While a particular formula with particular substituent groups is shown herein, the synthesis is applicable to all formulas and all substituent groups herein.
• Compounds of formula (XI), wherein RI , R ⁇ and R ⁇ are as defined herein, may be prepared from compounds of formula (XII), wherein R*, R ⁇ and R ⁇ are as defined herein and wherein X" is a leaving group such as a halogen atom, mesylate (methanesulfonate), tosylate (p- toluenesulfonate), or triflate (trifluoromethanesulfonate) (suitably a halogen atom such as a chlorine atom).
• the compounds of formula (XII), e.g. wherein X" is Cl, can be reacted with an azide salt such as sodium, lithium or potassium azide, in a suitable solvent such as dimethylsulfoxide such as dry DMSO, e.g. at a suitable temperature such as room temperature, to give compounds of formula (XI).
• an azide salt such as sodium, lithium or potassium azide
• a suitable solvent such as dimethylsulfoxide such as dry DMSO, e.g. at a suitable temperature such as room temperature
• Compounds of formula (XII), wherein Rl, R ⁇ and R ⁇ and X" are as defined herein, can be prepared by reaction of compounds of formula (XIII), wherein RI , R ⁇ and R ⁇ are as defined herein, with a suitable reagent such as thionyl chloride (for when X ⁇ is Cl), oxalyl chloride (for when X" is Cl), methanesulfonyl chloride (for when X" is mesylate), or/> ⁇ r ⁇ -toluenesulfonyl chloride (for when X" is tosylate) and Preferably thionyl chloride.
• a suitable reagent such as thionyl chloride (for when X ⁇ is Cl), oxalyl chloride (for when X" is Cl), methanesulfonyl chloride (for when X" is mesylate), or/> ⁇ r ⁇ -toluenesulfonyl chlor
• X" is Cl
• a suitable non-aqueous (e.g. anhydrous) aprotic organic solvent such as toluene, e.g. with heating to ca. 60-90 0 C for example ca. 85°C.
• Alternative conditions include reacting compounds of formula (XIII) with thionyl chloride and methanesulfonic acid in a suitable non-aqueous (e.g. anhydrous) aprotic organic solvent such as dichloromethane, e.g. at a suitable temperature such as room temperature.
• compounds of formula (XI) wherein Rl, R ⁇ and R ⁇ are as defined herein can be prepared directly from compounds of formula (XIII) wherein RI , R ⁇ and R ⁇ are as defined herein.
• compounds of formula (XI) may be prepared by reacting compounds of formula (XIII) with an azide salt, e.g. sodium azide, in the presence of a halogenating agent such as carbon tetrabromide and a phosphine such as triphenylphosphine under suitable conditions, such as N,N-dimethylformamide, e.g. at a suitable temperature such as between 0 0 C and room temperature (see e.g. Toyota et. al. Journal of Organic Chemistry (2000), 65(21), 7110-7113).
• an azide salt e.g. sodium azide
• a halogenating agent such as carbon tetrabromide
• a phosphine such as triphenylphosphine
• suitable temperature such as between
• X" can in particular be a chlorine atom.
• a benzenesulfonate salt of the compound of formula (XII) can for example be used, in particular when R ⁇ and R ⁇ are ethyl and when R ⁇ is, for instance, a tetrahydro-2H-pyran-4-yl.
• reaction of the compound (XII) or the salt thereof to the amine compound (IX) or the salt thereof may optionally be carried out under suitable conditions, for example by reaction of a compound of formula (XII) or a salt thereof with an aminating agent.
• R ⁇ represents a hydrogen atom
• a suitable aminating agent may be used, e.g. an alkali-metal hexamethyldisilazide such as lithium hexamethyldisilazide, sodium hexamethyldisilazide or potassium hexamethyldisilazide (in particular lithium hexamethyldisilazide, e.g.
• a suitable non-aqueous non-alcohol (aprotic) organic solvent e.g. anhydrous solvent
• a suitable temperature such as about 25 to about 50 0 C, for example ca. 30-45 0 C or ca. 30-40 0 C.
• the reaction with the suitable aminating agent e.g. with the alkali-metal hexamethyldisilazide
• an aqueous acid such as aqueous hydrochloric acid (e.g. 2- 10M, e.g. about 5M), for example at a suitable temperature such as from 0 0 C to room temperature, for example at 5-15 0 C or ca.
• aqueous base such as cone. (e.g. 32% w/w) NaOH solution
• a mono-acid-addition salt e.g. monohydrochloride
• a suitable acid such as HCl (e.g. aqueous hydrochloric acid such as ca. 36% w/w aq. HCl).
• the precursor alcohol compound of formula (XIII) or a salt thereof is converted into the amine of formula (IX) or a salt thereof, via the compound of formula (XII) or a salt thereof, without substantially purifying and/or without substantially isolating the compound of formula (XII) or the salt thereof wherein X" is a chlorine atom.
• the compound of formula (XII) or the salt thereof wherein X" is a chlorine atom can for example be in the form of the benzenesulfonate salt, in particular when
• RI and R ⁇ are ethyl and when R ⁇ is for instance, a tetrahydro-2H-pyran-4-yl:
• RI , R ⁇ and R ⁇ are as defined herein
• X ⁇ is an alkyl group such as a Cj .5 or Cj .4 alkyl (e.g. straight-chain alkyl) group e.g. in particular ethyl, with a suitable reducing agent in a suitable solvent, e.g. at a suitable temperature.
• a suitable reducing agent is lithium borohydride, in which case: a suitable solvent can be a mixture of tetrahydrofuran (e.g. dry) and methanol (e.g.
• a suitable reaction temperature can be from room temperature to the reflux temperature, e.g. about 50 to about 75 0 C, e.g. about 60 to about 70 0 C, e.g. 63-69 0 C or 64-68 0 C.
• Another reducing agent is di-z ' so-butylaluminium hydride (e.g. solution in toluene), in which case: a suitable solvent is dichloromethane and/or toluene, and/or a suitable reaction temperature can be about 0 0 C.
• Compounds of formula (XIV), wherein R ⁇ , R ⁇ and R ⁇ and X' are as defined herein, may be prepared by reaction of a compound of formula (XV) with an amine of formula R ⁇ NH2, for example generally according to the method described by Yu et. al. in J. Med Chem., 2001, 44, 1025-1027.
• the reaction is preferably carried out in the presence of a base such as triethylamine or N,N-diisopropylethylamine, and/or in an organic solvent such as ethanol, dioxane, l-methyl-2- pyrrolidinone (NMP) or acetonitrile.
• the reaction may require heating e.g. to about 60-180 0 C, for example at 115°C:
• R ⁇ is a N-aminocarbonyl-piperidinyl or N-aminocarbonyl-pyrrolidinyl group
• the urea-forming reagent may be benzyl isocyanate (followed later by debenzylation e.g. reductive debenzylation), or preferably the urea- forming reagent is tri(Cj _4alkyl)silyl isocyanate such as a tri(Cj_2alkyl)silyl isocyanate, preferably trimethylsilyl isocyanate.
• the conversion of the compound (XIVa) or salt thereof to the compound (XIV) may be carried out in the presence of a suitable base such as N,N-diisopropylethylamine, in a suitable solvent such as dichloromethane or chloroform, at a suitable temperature such as at room temperature or at the reflux temperature of the solvent.
• a suitable base such as N,N-diisopropylethylamine
• a suitable solvent such as dichloromethane or chloroform
• Compound (XIVa), wherein RI , R ⁇ , ⁇ 7 and n ⁇ are as defined herein, or the salt thereof can be prepared from compound (XIVb) below, wherein wherein R*, R% X ⁇ and ⁇ r are as defined herein and Prot is a suitable nitrogen protecting group such as (tert-butyloxy)carbonyl, by removal of the nitrogen protecting group.
• suitable nitrogen protecting group such as (tert-butyloxy)carbonyl
• suitable acidic conditions such as with hydrogen chloride (e.g. 4M) in a suitable solvent such as 1,4-dioxane:
• the reaction is optionally carried out in the presence of a base such as triethylamine or NN- diisopropylethylamine , optionally in a suitable organic solvent such as acetonitrile, at a suitable temperature such as 60-100 0 C (e.g. 80-90 0 C).
• a base such as triethylamine or NN- diisopropylethylamine
• a suitable organic solvent such as acetonitrile
• Suitable conditions for reaction of compounds of formula (XVI) with a dialkyl (l-chloroalkylidene)propanedioate of formula (XVII) include heating in a suitable solvent such as toluene, in the presence of a suitable base such as triethylamine, at a suitable temperature such as the reflux temperature of the solvent.
• Suitable conditions for the reaction of the intermediate with phosphorous oxychloride include heating at the reflux temperature of phosphorous oxychloride.
• Compounds of formula (XVII), wherein R ⁇ and X ⁇ are as defined herein, may be prepared by reaction of compounds of formula (XVIII) , wherein R ⁇ and X ⁇ are as defined herein, with phosphorus oxychloride in the presence of a suitable base such as tributylamine, at a suitable temperature such as 80-130 0 C, for example about 100-120 0 C.
• a suitable base such as tributylamine
• Compounds of formula (XVIII), wherein R ⁇ and X ⁇ are as defined herein, may be prepared by reaction of a dialkyl malonate of formula (XIX), wherein X ' is as defined herein, with magnesium chloride and a suitable base such as triethylamine, in a suitable solvent such as acetonitrile, at a suitable temperature such as 5-10 0 C, followed by addition of an acid chloride of formula (XX), for example propanoyl chloride, at a suitable temperature such as between 10 0 C and room temperature.
• a dialkyl malonate of formula (XIX) wherein X ' is as defined herein
• magnesium chloride and a suitable base such as triethylamine
• a suitable solvent such as acetonitrile
• Compounds of formulae (XIX) and (XX) are either known compounds or may be prepared by conventional means.
• compounds of formulae (XIX) and (XX) where X ⁇ and R ⁇ respectively represent ethyl are available from Aldrich.
• Compounds of formula (XV), wherein Rl, R ⁇ and X ⁇ are as defined herein may alternatively be prepared by reaction of a compound of formula (XVI), wherein RI is as defined herein, with compounds of formula (XXI), wherein R ⁇ and X ⁇ are as defined herein, with heating, followed by reaction with phosphorous oxychloride, again with heating (see Yu et. al. in J. Med Chem., 2001, 44, 1025-1027).
• Compounds of formula (XXI) can for example be diethyl [(ethyloxy)methylidene]propanedioate (wherein R ⁇ is H and X ⁇ is Et, available from Aldrich) or diethyl [l-(ethyloxy)ethylidene]propanedioate (wherein R ⁇ is Me and X ⁇ is Et, see Eur. Pat. Appl. (1991), EP 413918 A2).
• R.4 ⁇ should be chosen so as to contain one less carbon atom than R ⁇ , for example R ⁇ is methyl will afford R ⁇ as ethyl.
• the 4-chloro substituent in the compound of formula (XV) can be replaced by another halogen atom, such as a bromine atom, or by another suitable leaving group which is displaceable by an amine of formula R- ⁇ NH 2
• the leaving group can, for example, be an alkoxy group -OR 35 such as -0Ci_ 4 alkyl (in particular -OEt) or a group -
• reaction may be carried out with or without solvent and may require heating.
• (aa) may alternatively be prepared from compounds for formula XXXVIII, wherein R ⁇ and R ⁇ are as defined herein, n ⁇ is 0 or 1, and Proc represents a suitable protecting group such as tert- butoxycarbonyl.
• Suitable conditions include treatment suitable acidic conditions such as hydrogen chloride in a suitable solvent such as 1,4-dioxane at a suitable temperature such as room temperature.
• Compounds for formula XXXVIII, wherein R ⁇ and R ⁇ , rr and Proc are as defined herein, may be prepared from compounds for formula XXXIX, wherein RI and R % r? and Proc are as defined herein.
• Suitable conditions include reaction of compounds of formula XXXIX with an azide such as sodium azide and a halogenating agent such as carbon tetrabromide, in the presence of a suitable phosphine such as triphenylphosphine, in a suitable solvent such as NN,- dimethylformamide, at a suitable temperature such as between 0 0 C and room temperature.
• Compounds of formula (XXXIX), wherein RI and R ⁇ , n ⁇ and Proc are as defined herein, may be prepared from compounds of formula (XL), wherein R ⁇ and R ⁇ , rr, Proc and X ⁇ are as defined herein, by reduction with a suitable reducing agent such as lithium borohydride, in a suitable solvent such as a mixture of tetrahydrofuran and methanol, at a suitable temperature such as at the reflux temperature of the solvent.
• a suitable reducing agent such as lithium borohydride
• Compounds of formula (XL), wherein R ⁇ and R ⁇ , rr, Proc and X ⁇ are as defined herein, may be prepared from compounds of formula (XV), wherein Rl, R% and X ⁇ are as defined herein, by reaction of a compound of formula (XV) with an amine of formula (XLI), wherein Proc and rr are as defined herein.
• the reaction is preferably carried out in the presence of a base such as triethylamine or N,N-diisopropylethylamine, and/or in an organic solvent such as ethanol, dioxane, 1 -methyl-2-pyrrolidinone (NMP) or acetonitrile.
• the reaction may require heating e.g. to about 60-180 0 C, for example at 120 0 C:
• Scheme 1 describes the general synthesis of the compounds 1-5.
• Compound 1-1 above is treated with phenyl chloridocarbonate in dichloromethane and a tertiary amine such as triethylamine or diisopropylethylamine to give intermediate 1-2.
• Intermediate 1-2 is then treated with the suitably protected amine 1-3 in dichloromethane and a tertiary amine such as triethylamine or diisopropylethylamine to give intermediate 1-4.
• a suitable protecting group is needed when R 6 in compound 1-3 contains a primary or secondary amine.
• Intermediate 1-4 is then deprotected in a method defined by the nature of the protecting group used. In the case of an acid labile amine protecting group like Boc, deprotection can be achieved using a strong acid such as TFA in a solvent such as dichloromethane to give 1-5.
• Scheme 2 describes an alternate synthesis of compounds 2-6.
• Intermediate 2-1 is treated with the amine 2-2 in dichloromethane and a tertiary amine such as triethylamine or diisopropylethylamine to give intermediate 2-3.
• a palladium catalyst such as Pd(PPh 3 ) 4
• Pd(OAc) 2 / PPh 3 gives intermediate 2-5.
• a palladium catalyst such as Pd(PPh 3 ) 4 , or Pd(OAc) 2 / PPh 3
• the resulting intermediate 2-5 is then deprotected in a method defined by the nature of the protecting group used.
• an acid labile amine protecting group like Boc
• deprotection can be achieved using a strong acid such as TFA in a solvent such as dichloromethane to give 2-6.
• Scheme 3 describes an alternate synthesis of compounds 3-5.
• Suzuki coupling of intermediate 3-1 with a boronic acid or boronic ester aldehyde 3-2 (R is boronic acid, boronic ester) in the presence of a palladium catalyst, such as Pd(PPh 3 ) 4 , or Pd(OAc) 2 / PPh 3 gives intermediate 3-3.
• Stille coupling of 3-2 with a trialkyl tin aldehyde R is trialkyl tin
• a palladium catalyst such as Pd(PPh 3 ) 4
• Pd(OAc) 2 / PPh 3 gives intermediate 3-3.
• a variety of reverse phase columns e.g., Luna 5u C 18(2) 10OA, SunFireTM Cl 8, XBridgeTM Cl 8 were used in the purification with the choice of column support dependent upon the conditions used in the purification.
• the compounds are eluted using a gradient of acetonitrile and water.
• Neutral conditions used an acetonitrile and water gradient with no additional modifier
• acidic conditions used an acid modifier, usually 0.1 % TFA (added to both the acetonitrile and water)
• basic conditions used a basic modifier, usually 0.1 % NH 4 OH (added to the water).
• Analytical hplc was run using an Agilent system with variable wavelength UV detection using reverse phase chromatography with an acetonitrile and water gradient with a 0.05 or 0.1 % TFA modifier (added to each solvent).
• LC-MS was determined using either a PE Sciex Single Quadrupole LC/MS API- 150 or a Waters.
• the compound is analyzed using a reverse phase column, e.g., Thermo Aquasil/Aquasil C 18, Acquity UPLC C 18, Thermo Hypersil Gold eluted using an acetonitrile and water gradient with a low percentage of an acid modifier such as 0.02% TFA or 0.1 % formic acid.
• Heating of reaction mixtures with microwave irradiations was carried out on a Smith Creator (purchased from Personal Chemistry, Forboro, MA, now owned by Biotage), an Emrys Optimizer (purchased from Personal Chemistry) or an Explorer (purchased from CEM, Matthews, NC) microwave.
• Cartridges or columns containing polymer based functional groups can be used as part of compound workup.
• the "amine” columns or cartridges are used to neutralize or basify acidic reaction mixtures or products. These include NH2 Aminopropyl SPE-ed SPE Cartridges available from Applied Separations and diethylamino SPE cartridges available from United Chemical Technologies, Inc.
• the organic layer was separated and the aqueous layer was extracted with CHCI3 (150 mL x 2).
• NaBH 4 (2.4 g, 64.3 mmol) was added cautiously to a solution of NiCl 2 (2.8 g, 21.6 mmol), BoC 2 O (9.6 g, 44.0 mmol) and 5-bromo-2-methylbenzonitrile (4.2 g, 21.4 mmol) in EtOH (150 mL) at 0 0 C within 0.5 h, then stirred for 40 min. After the reaction had subsided, the mixture was left to stir at room temperature for 0.5 h. Then the solvent was removed and the residue was dissolved in AcOEt and a saturated solution of NaHC ⁇ 3, then filtered and washed with AcOEt. The combined organic layers were washed with brine and dried over Na 2 SO 4 .
• Potassium phthalate (28.8 g, 0.16 mol) was added to a solution of l-bromo-3- (bromomethyl)-2-(methyloxy)benzene (41.4 g, 0.15 mol) in DMF (350 mL). The mixture was heated to 90 0 C over night. Then the solvent was removed under reduced pressure. The residue was dissolved in CHCl 3 (300 mL), and filtered. The filtrate was washed with H 2 O (100 mL x T), brine (100 mL), and dried over Na 2 SO 4 .
• tert-Butyl dimethylsilyl chloride (18.7 g, 124.3 mmol), Et 3 N (14.08 g, 139.2 mmol) and DMAP (194.3 mg, 8.9 mmol) were dissolved in CH 2 Cl 2 (120 mL) and the solution was cooled to 0- 5 0 C.
• Pd(OAc) 2 (102.0 mg, 0.45 mmol, 0.03 eq.), PPh 3 (476.4 mg, 1.82 mmol, 0.12 eq.), K 2 C ⁇ 3 (3.14 g, 22.7 mmol, 1.50 eq.) and 2-[(3-bromo-5-methylphenyl)methyl]- lH-isoindole- l,3(2H)-dione (5.00 g, 13.1 mmol, 1.00 eq.) were suspended in anhydrous 1,4-dioxane (30 mL) under nitrogen.
• Triphenylphosphane (2.62 g, 10.0 mmol) was added to l-bromo-3-(bromomethyl)benzene (2.5 g, 10.0 mmol) in toluene (15 mL) and the mixture heated in a microwave at 100 0 C for about 1 h. The mixture was filtered to afford the title compound (4.55 g, 89 %).
• the reaction was quenched with water and then extracted with ethyl acetate twice.
• the combined organic layers were washed with water and brine.
• the organic layer was filtered through a 0.45 ⁇ syringe filter to remove residual Pd and then concentrated under vacuum to give the crude residue.
• the crude residue was purified with chromatography eluting with 0 to 100% ethyl acetate in hexane (product came out at 100% ethyl acetate).
• the mixture was degassed for 5 min and then heated under microwave for 30 min at 150 0 C. It was quenched with water and then extracted with ethyl acetate twice. The combined organic layer was washed with water and brine. The organic layer was passed through a pad of celite, washed with ethyl acetate and then concentrated under vacuum to give the crude residue. The crude residue was purified with chromatography eluting with 0 to 100 % ethyl acetate in DCM (product came out at 90% ethyl acetate in DCM).
• the mixture was degassed for 5 min and then heated under microwave for 10 min at 100 0 C. It was passed through PL-Thiol MP SPE+ to get rid of Pd content and then washed with saturated NaHCO 3 . The organic layer was concentrated under vacuum to give the crude residue. The crude residue was purified with chromatography eluting with 0 to 100 % ethyl acetate in DCM (product came out at 100% ethyl acetate).
• the mixture was degassed for 5 min and then heated under microwave for 10 min at 100 0 C. It was passed through a pad of Celite and then washed with ethyl acetate. The organic layer was washed with water and brine. It was concentrated under vacuum to give the crude residue.
• the crude residue was purified with chromatography eluting with 0 to 100 % ethyl acetate in DCM (product came out at 90% ethyl acetate in DCM).
• the mixture was degassed for 5 min and then heated under microwave for 10 min at 100 0 C. It was passed through PL-Thiol MP SPE+ to get rid of Pd content and then washed with ethyl acetate. The organic layer was washed with water and brine. It was concentrated under vacuum to give the crude residue. The crude residue was purified with chromatography eluting with 0 to 100 % ethyl acetate in DCM (product came out at 90% ethyl acetate in DCM).
• Example 111 N- ⁇ [l,6-Diethyl-4-(tetrahydro-2H-pyran-4-ylamino)-l//-pyrazolo[3,4- 6]pyridin-5-yl]methyl ⁇ -iV-[(6-fluoro-3'- ⁇ [(35)-3-methyl-l- piperazinyl] methyl ⁇ -3-biphenylyl)methyl] urea and ⁇ yV-bis ⁇ [1 ,6-diethyl-4- (tetrahy dro-2//-py ran-4-ylamino)- l//-py razolo [3 ,4-b] py ridin-5- yl] methyl ⁇ urea
• Example 112 ⁇ - ⁇ [l ⁇ -DiethyM- ⁇ etrahydro-lH-pyran ⁇ -ylaminoJ-lH-pyrazoloP ⁇ - 6]pyridin-5-yl]methyl ⁇ -iV- ⁇ [3'-(4-piperidinylmethyl)-3- biphenylyl]methyl ⁇ urea
• the crude residue was purified with a Gilson ⁇ PLCeluting with 10 to 70% CH 3 CN in water in a flowrate of 20 mL/min.
• the product fractions were dried with EZ GeneVac and combined into one batch with DCM. 25% TFA in DCM was added to the intermediate and stirred at room temperature for 3 h.
• the crude product was purified with prepative HPLC eluting with 10 to 70% CH 3 CN in water in a flowrate of 20 mL/min.
• the product fractions were combined and then free based with saturated NaHCO 3 .
• the reaction mixture was passed though a PL-Thiol MP SPE+ column to get rid of Pd content and washed with ethyl acetate. It was then quenched with water and then extracted with ethyl acetate twice. The combined organic layer was washed with brine and then concentrated under vacuum to give crude product. It was then purified with a Gilson HPLC (with 1 % TFA in the solvent) eluting with 10 to 70% CH 3 CN in water in a flow rate of 20 mL/min. The product fractions were dried using a GeneVac to give the crude intermediate. 25% TFA in DCM was added to the intermediate and stirred at room temperature for about 3 h.
• the reaction mixture was passed though a PL-Thiol MP SPE+ column to get rid of Pd content and washed with ethyl acetate. It was then quenched with water and then extracted with ethyl acetate twice. The combined organic layer was washed with brine and then concentrated under vacuum to give crude product. It was then purified with a Gilson ⁇ PLC (with 1% TFA in the solvent) eluting with 10 to 70% CH 3 CN in water in a flow rate of 20 mL/min. The product fractions were dried using a GeneVac to give the crude intermediate. 25% TFA in DCM was added to the intermediate and stirred at room temperature for about 3 h.
• the mixture was degassed by bubbling argon though it for 5 min and was then heated in a Biotage microwave at normal absorption for 10 min at 100 0 C.
• the crude mixture was filtered though a PL-Thiol MP SPE+ and was then washed with ethyl acetate and water.
• the organic layer was concentrated under vacuum to obtain the crude residue. It was purified with a Gilson ⁇ PLC (with 0.1 % TFA in the solvents) eluting with 10 to 70 % CH 3 CN in water in a flow rate of 20 mL/min.
• Solvent was evaporated under a stream of nitrogen and then purified with a Gilson ⁇ PLC (with 0.1 % TFA in the solvents) eluting with 10 to 70 % CH 3 CN in water in a flow rate of 20 mL/min.
• the product fractions were combined and free based with saturated NaHCO 3 and extracted with ethyl acetate twice.
• the mixture was degassed by bubbling argon though it for 5 min and was then heated in a Biotage microwave at normal absorption for 10 min at 100 0 C.
• the crude mixture was filtered though a PL-Thiol MP SPE+ and was then washed with ethyl acetate and water.
• the organic layer was concentrated under vacuum to obtain the crude residue. It was purified with a Gilson HPLC (with 0.1 % TFA in the solvents) eluting with 10 to 70 % CH 3 CN in water in a flow rate of 20 mL/min.
• the product fractions were combined and free based with saturated NaHCO 3 and extracted with ethyl acetate twice.
• the mixture was degassed by bubbling argon though it for 5 min and was then heated in a Biotage microwave at normal absorption for 10 min at 100 0 C.
• the crude mixture was filtered though a PL-Thiol MP SPE+ and was then washed with ethyl acetate and water.
• the organic layer was concentrated under vacuum to obtain the crude residue. It was purified with a Gilson ⁇ PLC (with 0.1 % TFA in the solvents) eluting with 10 to 70 % CH 3 CN in water in a flow rate of 20 mL/min.
• the product fractions were combined and free based with saturated NaHCO 3 and extracted with ethyl acetate twice.
• the mixture was degassed by bubbling argon though it for 5 min and was then heated in a Biotage microwave at normal absorption for 10 min at 100 0 C.
• the crude mixture was filtered though a PL-Thiol MP SPE+ and was then washed with ethyl acetate and water.
• the organic layer was concentrated under vacuum to obtain the crude residue. It was purified with a Gilson ⁇ PLC (with 0.1 % TFA in the solvents) eluting with 10 to 70 % CH 3 CN in water in a flow rate of 20 mL/min.
• the product fractions were combined and free based with saturated NaHCO 3 and extracted with ethyl acetate twice.
• Example 120 ⁇ - ⁇ [lj ⁇ -DiethyM- ⁇ etrahydro-lH-pyran ⁇ -ylaminoJ-lH-pyrazoloP ⁇ - ⁇ lpyridin-S- yl]methyl ⁇ -7V-( ⁇ 6-(methyloxy)-3'-[(l-methyl-4-piperidinyl)methyl]-3-biphenylyl ⁇ methyl)urea
• the mixture was degassed by bubbling argon though it for 5 min and was then heated in a Biotage microwave at normal absorption for 10 min at 100 0 C.
• the crude mixture was filtered though a PL-Thiol MP SPE+ and was then washed with ethyl acetate and water.
• the organic layer was concentrated under vacuum to obtain the crude residue. It was purified with a Gilson HPLC (with 0.1 % TFA in the solvents) eluting with 10 to 70 % CH 3 CN in water in a flow rate of 20 mL/min.
• the product fractions were combined and free based with saturated NaHCO 3 and extracted with ethyl acetate twice.
• the mixture was degassed by bubbling argon though it for 5 min and was then heated in a Biotage microwave at normal absorption for 10 min at 100 0 C.
• the crude mixture was filtered though a PL-Thiol MP SPE+ and was then washed with ethyl acetate and water.
• the organic layer was concentrated under vacuum to obtain the crude residue. It was purified with a Gilson ⁇ PLC (with 0.1 % TFA in the solvents) eluting with 10 to 70 % CH 3 CN in water in a flow rate of 20 mL/min.
• the product fractions were combined and free based with saturated NaHCO 3 and extracted with ethyl acetate twice.
• Examples 123-125 Using array chemistry, following the procedure as described above in the preparation of N- ⁇ [1,6- diethyl-4-(tetrahydro-2H-pyran-4-ylamino)-lH-pyrazolo[3,4-b]pyridin-5-yl]methyl ⁇ -N'-[(6-fluoro- 3'- ⁇ [(3S)-3-methyl-l-piperazinyl]methyl ⁇ -3-biphenylyl)methyl]urea (Example 122), N- ⁇ [1,6- diethyl-4-(tetrahydro-2H-pyran-4-ylamino)-lH-pyrazolo[3,4-b]pyridin-5-yl]methyl ⁇ -N'-[(6-fluoro- 3'-formyl-3-biphenylyl)methyl]urea was reacted with an appropriate amine to give Examples 123- 125 listed in Table 1.
• N'-[(3'-formyl-6-methyl-3-biphenylyl)methyl]urea 40 mg, 0.072 mmol
• 1 , 1 -dimethylethyl (2R)-2- methyl- 1 -piperazinecarboxylate 139.39 mg, 0.72 mmol, 10 eq
• acetic acid 4.13 ⁇ L, 0.072 mmol, 1 eq
• the mixture was stirred in VX-2500 Multi-Tube Vortexer over the weekend at room temperature.

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