Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/02—Separating microorganisms from their culture media
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01D—SEPARATION
  • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
  • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
  • B01D21/267—Separation of sediment aided by centrifugal force or centripetal force by using a cyclone
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
  • B03D—FLOTATION; DIFFERENTIAL SEDIMENTATION
  • B03D1/00—Flotation
  • B03D1/14—Flotation machines
  • B03D1/1412—Flotation machines with baffles, e.g. at the wall for redirecting settling solids
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
  • B03D—FLOTATION; DIFFERENTIAL SEDIMENTATION
  • B03D1/00—Flotation
  • B03D1/14—Flotation machines
  • B03D1/1418—Flotation machines using centrifugal forces
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
  • C12M21/02—Photobioreactors
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
  • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/12—Unicellular algae; Culture media therefor
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01D—SEPARATION
  • B01D2221/00—Applications of separation devices
  • B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
  • B04C—APPARATUS USING FREE VORTEX FLOW, e.g. CYCLONES
  • B04C3/00—Apparatus in which the axial direction of the vortex flow following a screw-thread type line remains unchanged ; Devices in which one of the two discharge ducts returns centrally through the vortex chamber, a reverse-flow vortex being prevented by bulkheads in the central discharge duct
  • B04C2003/006—Construction of elements by which the vortex flow is generated or degenerated

Definitions

• the invention relates to a method for concentrating from a flowing liquid microbiological organisms suspended in the liquid.
• algae have been found to have a number of advantageous properties.
• types of algae whose mass consists of as much as 50% oil, while • depending on the intended application - other advantageous substances such as dyes and the omega fatty acid EPA are also present in the algae.
• Another advantage is that the production capacity (the photosynthesis) in the production of algae can be particularly high.
• the production of algae per unit area has been found in practice to be as much as 100 to 150 times more efficient than the cultivation of a number of agricultural crops such as for instance maize and soya.
• the algae can also be used to purify waste water, and CO2 from for instance flue gases or fermentation waste gases can here be bonded. See for this for instance the ECN report “Duurzame co- productie vanfljnchemicalien en energie uit micro-algen (Renewable co-production of fine chemicals and energy from microalgae) ", public final report E.E.T. project K99005/398510-1010 by J.H. Reith, April 2004.
• a drawback of the production of algae is however that, as microbiological organisms, they are not easy to separate from the liquid in which they develop. Due to the generally very small dimensions of the microbiological organisms, the separation is difficult and expensive. Flotation, centrifugation, sand filtration and membrane technology are referred to as possible separating techniques. Chemical extraction is also recommended for the extraction of determined substances. All these separating techniques are only possible for high-quality application of the products to be separated. For relatively low- quality application of the microbiological organisms (more in particular as bio-fuel) these separating techniques are all too costly, and moreover do not even usually produce a positive (or even very slightly advantageous) energy balance.
• Centrifugation is thus for instance an energetically less favourable separating technique as centrifugation is an energy-intensive process.
• the method comprises the steps of: feeding a low-pressure liquid flow tangentially to a stationary cyclone; rotating the liquid in the stationary housing of the cyclone; and discharging at least two different fractions from the stationary cyclone.
• the cyclone described in this document is provided with a filter element for the purpose of also separating from the liquid particles lighter than the specific mass of the liquid. This technique is not suitable either for the purpose of commercial concentration of suspended microbiological organisms from a flowing liquid for relatively low-quality application of the microbiological organisms (such as the above stated bio-fuel).
• the present invention has for its object to provide a more efficient method of concentrating from a flowing liquid microbiological organisms suspended in the liquid.
• the invention provides for this purpose a method for concentrating from a flowing liquid microbiological organisms suspended in the liquid as according to claim 1.
• a lighter fraction will migrate to the inner side of the cyclone in at least substantially efficient manner, and a heavier fraction will migrate to the outer side of the cyclone.
• the heavier fraction and the lighter fraction are discharged from the cyclone at spaced-apart positions.
• the feed of flowing liquid with suspended microbiological organisms can now also take place radially and/or axially as desired in very efficient manner, i.e. either radially or axially or a combination of radially and axially.
• Another reason why precisely the presence of at least one guide element now results in said efficient concentration is that, as a result of the at least one guide element, the flow pattern in the vortex is relatively stable compared to a prior art vortex in which such a guide element is absent.
• a cyclone is a centrifugal separator in which a heavier fraction is flung to the outer side by the centrifugal force as a result of the greater mass.
• the different fractions usually leave the cyclone via different sides.
• Coagulation is here understood to mean droplets coalescing into a larger drop. This property can be influenced by for instance the composition of a mixture, the pH value, the presence of additives and so on.
• the present invention now provides the insight that it is possible to concentrate microbiological organisms in a stationary cyclone in viable manner. Concentration not only refers to the complete separation but also to the pre-separation (increasing the concentration of microbiological organisms suspended in a liquid). A correct dimensioning of the vortex must first be chosen here, for instance a diameter smaller than 20 millimetres. This because the vortex must develop a sufficient centrifugal force to concentrate the small, non-coagulating microbiological organisms, and the flow pattern must moreover be such that re-mixing is prevented.
• a complete separation is not essential but that a substantial increase in the concentration of microbiological organisms in a first fraction of the liquid flow is already very advantageous. A doubling of the concentration of for instance a few hundred milligrams per litre already results in a considerable increase in the efficiency of the overall separating process since the subsequent separating steps can now after all be performed more efficiently.
• the flow pattern in the vortex is very stable; this of course in order to prevent the very rapidly occurring re-mixing.
• stabilization of the flow pattern can for instance also be obtained by applying guide elements and/or stabilizing elements in the vortex. It is important here to keep the local Reynolds number as low as possible everywhere, whereby preferably no (heavily) turbulent flow occurs in the vortex.
• the liquid is fed to the device from a plurality of sides. All these measures contribute toward the flow of the medium mixture to be fed to the cyclone having a substantially stable flow pattern during processing step A).
• the separating space in the vortex usually has an elongate form which has an inner side of circular cross-section (i.e. in a cross-section perpendicularly of the longitudinal or lengthwise axis of the cyclone).
• the separating space can optionally be tapering and can be provided as desired with a core around which the mixture is set into rotation as a vortex.
• the liquid in the stationary housing of the cyclone can also follow a fully helical flow path (the structure need not be tapering here, but can also be cylindrical).
• the rotating flow can be generated by a helical structure.
• the liquid is preferably formed by water with microorganisms dispersed therein. Depending on the conditions, this can be fresh, brackish or salt water.
• gas bubbles are created in the liquid.
• gas bubbles are preferably present in the liquid during rotation of the liquid in the stationary housing of the cyclone.
• the thus present (micro)bubbles adhere to the microbiological organisms, whereby the difference in mass density of the fractions for separating can be changed, as a result of which a simpler separation becomes possible.
• This effect can otherwise also be obtained (or be enhanced) by causing the liquid with microbiological organisms suspended therein to expand during the feed to the stationary housing of the cyclone. The expansion can take place during the feed or at any moment the liquid is already situated in the stationary housing.
• a flocculant for the microbiological organisms is added to the flowing liquid with suspended microbiological organisms.
• a flocculant results in the small microbiological organisms joining together into larger complexes (flocculent precipitates) which are S easier to concentrate (or can even be separated from the liquid).
• the flocculant can have a chemical composition, although it is preferably formed by a natural flocculant such as for instance Chitosan. It is otherwise noted here that microbiological organisms can also coalesce, bind or clot by means of other phenomena.
• FIG 1 shows a cut-away perspective view of a device for performing the method according to the invention
• figure 2 shows a cut-away perspective view of an alternative embodiment variant of a5 device for performing the method according to the invention.
• Figure 1 shows a vortex 20 with a core 1 and a casing 2, between which guide fins 3 are positioned.
• the liquid with suspended microbiological organisms for instance algae
• the method of feeding the liquid is of minor importance; the liquid can also have a radial component upstream of guide fins 3 (see for instance arrow Pl').
• stabilizing fins 4 can additionally be arranged between guide fins 3. As a consequence of guide fins 3 and stabilizing fins 4 the local Reynolds number will be lower, with the result of a stable flow more quickly at that location.
• Botryococcus braunii has for instance a mass density lower than that of water, whereby it can be separated as the lighter fraction, and bio-fuel can thus for instance be produced in very efficient manner. Separation of the lighter fraction is generally more advantageous in a cyclone than separation of the heavier fraction.
• Separating space 9 is bounded by core 1 and casing 2.
• the periphery of the distal end of core 1 decreases gradually by means of an end part 8 provided with a decreasing diameter.
• a tail section S is also provided in order to increase the retention time in vortex 20. This tail section S has an elongate form and is here shown in conical form, although it can for instance also take a cylindrical form.
• a critical value in practice relates to the distal side of tail section S which must preferably be smaller than 20 mm, more preferably even smaller than 15, 10 or 5 mm.
• the advantage of the presence of tail section S is that maintaining the momentum further increases the centrifugal force, and this results in an improved separation.
• the liquid with increased concentration of microbiological organisms will move in axial direction via a wall 10 of tail section 5 toward an outlet 6, where the first fraction of liquid with the increased concentration of microbiological organisms leaves vortex 20 as according to arrow P3.
• the second fraction of the liquid with a decreased concentration of microbiological organisms will leave vortex 20 rearward as according to arrow P4 through an opening 7 arranged in the core. Because of the fractions exiting in different directions as according to arrows P3 and P4 the vortex 20 is also referred to as a counterflow device.
• Figure 2 shows a vortex 30, the components of which corresponding with the components as shown in vortex 20 according to figure 1 are designated with the same reference numerals.
• Guide fins 3 are once again positioned between a core 1 and a casing 2, and the liquid with suspended microbiological organisms is fed substantially axially as according to arrow PI such that due to guide fins 3 it obtains a rotating tangential component as according to arrow P2.
• Stabilizing fins 4 can once again be arranged for further stabilization of the flow. It is once again assumed here by way of example that the microbiological organisms have a higher density than the liquid.
• the microbiological organisms for instance algae
• Core 1 is again provided with an end part 8 with gradually decreasing diameter, and a tail section S is also provided.
• the liquid with increased concentration of microbiological organisms will move in axial direction via a wall 10 of tail section S to an outlet 11, where the first fraction of liquid with the increased concentration of microbiological organisms leaves vortex 30 as according to arrow P5.
• the second fraction of the liquid with a decreased concentration of microbiological organisms will likewise leave vortex 30 on the distal side from the centre of tail section 5 through discharge element 12 as according to arrow P6. Because of the fractions exiting in different directions as according to arrows P5 and P6 the vortex 30 is also referred to as a vortex of the throughfiow type.

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• Life Sciences & Earth Sciences (AREA)
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• Bioinformatics & Cheminformatics (AREA)
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• General Engineering & Computer Science (AREA)
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• Tropical Medicine & Parasitology (AREA)
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• Chemical Kinetics & Catalysis (AREA)
• Botany (AREA)
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• Molecular Biology (AREA)
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• Physical Water Treatments (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)

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• 2007
  • 2007-05-15 NL NL2000649A patent/NL2000649C2/nl not_active IP Right Cessation
• 2008
  • 2008-05-08 EP EP08753760A patent/EP2155352A1/en not_active Withdrawn
  • 2008-05-08 WO PCT/NL2008/050277 patent/WO2008140307A1/en active Application Filing
  • 2008-05-08 MX MX2009012356A patent/MX2009012356A/es unknown
  • 2008-05-08 AU AU2008251119A patent/AU2008251119A1/en not_active Abandoned
  • 2008-05-08 BR BRPI0810264-3A2A patent/BRPI0810264A2/pt not_active Application Discontinuation

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