EP2084260A1 - Procédé et dispositif de gazage, en particulier pour la fermentation microbiologique et la culture de cellules - Google Patents

Procédé et dispositif de gazage, en particulier pour la fermentation microbiologique et la culture de cellules

Info

Publication number
EP2084260A1
EP2084260A1 EP07819323A EP07819323A EP2084260A1 EP 2084260 A1 EP2084260 A1 EP 2084260A1 EP 07819323 A EP07819323 A EP 07819323A EP 07819323 A EP07819323 A EP 07819323A EP 2084260 A1 EP2084260 A1 EP 2084260A1
Authority
EP
European Patent Office
Prior art keywords
reactor
gassing
elements
agitator shaft
gas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07819323A
Other languages
German (de)
English (en)
Inventor
Hanspeter Tschamber
Andrea Kloss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mut-tschamber Misch- und Trenntechnik GmbH
Original Assignee
Mut-tschamber Misch- und Trenntechnik GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mut-tschamber Misch- und Trenntechnik GmbH filed Critical Mut-tschamber Misch- und Trenntechnik GmbH
Publication of EP2084260A1 publication Critical patent/EP2084260A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/90—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms 
    • B01F27/902—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms  cooperating with intermeshing elements fixed on the receptacle walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/233—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
    • B01F23/2331—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements characterised by the introduction of the gas along the axis of the stirrer or along the stirrer elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element

Definitions

  • the invention relates to a method and a device for gassing, in particular for the microbiological fermentation and cultivation of cells in a closed space, according to the preamble of claim 1.
  • a device of this type is known for example from EP-AO 365 621.
  • a vertical agitator shaft is arranged with a number of superimposed in several levels Bega- sungs or stirring elements.
  • the gassing elements and Preferably, the stirring elements are hollow, connected to gas supply channels and provided with at least one gas-permeable part.
  • the agitator shaft protrudes with its one end through the reactor wall and is driven by an outside of the reactor arranged electric motor. For example, since a cell growing bioreactor must be hermetically sealed, the sealing of the rotating in the stationary reactor agitator or drive shaft is a real problem.
  • the present invention has for its object to provide a method with an optimized gassing of a liquid in the reactor and to simplify a device of the type mentioned constructive and sterile technology to improve.
  • the gassing elements are designed such that they can be made variable depending on the oxygen requirement of the product formers.
  • the gas bubbles must be uniformly introduced as the smallest possible bubbles in the liquid. Due to the areal to be adapted ventilation slots these requirements are met.
  • the inventive device is simple and inexpensive, since all sealing problems with respect to the rotating agitator shaft for Outside atmosphere omitted and a hermetically sealed reactor space can be ensured without much effort.
  • the device can also be designed to save space, since no external drive is needed.
  • gassing elements provided with inserts over the height of the reactor, which allow a metered gas supply.
  • FIG. 1 schematically shows an apparatus according to the invention for gassing with gassing or stirring elements arranged one above the other in four planes;
  • FIG. 2 shows a gassing element according to the invention according to FIG. 1 viewed from above;
  • FIG. 3 shows a cross section through the gassing element according to FIG. 2;
  • Fig. 4 shows an embodiment of an inventive Begasungsl. Stirring element in side view
  • FIG. 5 the gassing or stirring element of FIG. 4 in plan view.
  • FIG. 1 shows a reactor 1 which forms a bioreactor intended for microbiological fermentation and cultivation of cells.
  • a stirrer shaft 2 is rotatably arranged, which is held with its one end in a shaft suspension or mounting 3 and supported with the other in a bottom bearing 4.
  • the shaft suspension or storage 3 is assigned to a reactor cover Ia. - A -
  • a plurality of radially extending aeration elements 15 or agitating elements 10 are arranged one above the other in several planes, in this case in three planes aeration elements 15 and in the two intermediate planes agitating elements 10.
  • smart elements 17 are preferably provided on the inside of the reactor wall. which serve as a rotation prevention of the liquid and sometimes cause homogenization at low rotational speed of the agitator shaft 2.
  • the gassing elements 15 are designed in such a way that they are kept variable depending on the oxygen requirement of the product formation occurring through the gassing and that such gassing of the liquid in the reactor causes the gases to be uniformly introduced into the liquid as microbubbles preferably be introduced with a diameter of less than one millimeter. In addition, a homogeneous distribution of the bubbles in the liquid column should take place.
  • the gassing elements 15 each comprise an elongated hollow body with gas-permeable inserts 16 arranged approximately over the entire length on the lower and upper side.
  • the hollow body forming a respective gassing element 15 with a corresponding gas supply channel 11 is tubular in this case , so that the gas to be injected is guided to the end of the hollow body.
  • These gas-permeable inserts 16 are preferably made of a sintered metal, such as stainless steel. But they could also be made of ceramic or a plastic.
  • these gassing elements 15 distributed over the height of the reactor 1 are provided with inserts 16, which are provided either with differently sized surfaces or with different porosities, depending in particular on the oxygen requirement of the product formers.
  • the hydrostatic pressure of the liquid is taken into account.
  • the gassing elements 15 therefore, larger surfaces or a larger porosity of the inserts 16 are to be provided in the lower region of the reactor 1 than in the upper region.
  • the gassing elements 15 are supplied by a guided through the agitator shaft 2 gas supply channel 1 1, 12 with the gas.
  • the stirring elements 10 projecting radially away from the agitator shaft 2 are arranged at different levels. In principle, they could also be equipped with inserts 16 and at the same time serve as gassing elements. With them, the stirring effect for homogenization of the reactor contents is ensured, with a low rotational speed (5 to 100 U / min) can be selected, since in the subject method, a dispersion of the gas bubbles is not necessary.
  • a drive member 20 is provided with a wing portion 20a fixed at the end and a separate gas and / or air supply 21 with which this agitator shaft 2 is rotatable.
  • This wing part 20a has at least one, optionally three recoil nozzles 22, which are directed in the circumferential direction of the agitator shaft 2 substantially.
  • the wing part 20a provided with the recoil nozzles 20 is connected in each case via the separate gas supply channel 21 to a vertical gas supply channel 23 guided by the rotating agitator shaft 2, which in turn has a transfer unit 13 is in communication with a stationary in a reactor wall Ib installed gas supply line 24.
  • gas may be introduced into the wing portion 20a from a pressurized gas source in such an amount and under such a pressure that the gas exiting the recoil nozzles 20 promotes propulsion of the entire agitator, i. all with the agitator shaft 2 rotatably connected stirring and / or gassing elements 10, 15 causes.
  • the required amount of gas and the gas pressure as a pulse generator for the propulsion are achieved in the combination of the outside taking place Gasmengeregulleiter in coordination with a corresponding nozzle cross-section.
  • the drive of the agitator shaft 2 provided with the stirring and / or gassing alone on the propulsion causing remindstossdüsen 20 done, and the agitator shaft 2 does not need through the reactor wall be led to an outside drive. It is located inside the reactor 1, held in the shaft suspension 3 and guided in the bottom bearing 4, for example a Teflon bearing.
  • the bioreactor is hermetically sealed. The opening into the reactor 1 and installed in the reactor wall or in the reactor shell Ib stationary, preferably welded gas supply lines 14, 24 pose no risk of leakage.
  • the agitator shaft 2 could be via a known magnetic coupling (instead of the bottom bearing 4) additionally from a lying outside of the reactor 1 engine be driven without having to protrude the reactor wall.
  • the wing parts 10a advantageously have a fluid-technically optimized, aerodynamic shape, as indicated in FIGS. 4 and 5. This favors the rotational movement of the stirring elements 10th
  • the same gas can be used for propulsion, which is also provided for gassing the fermentation broth, e.g. Sterile air, pure oxygen or an oxygen / nitrogen mixture.
  • the device according to the invention for gassing, in particular for the microbiological fermentation and cultivation of cells in a closed space is simple and inexpensive, since all sealing problems with respect to the rotating agitator shaft are eliminated and a hermetically sealed reactor space can be ensured without much effort.
  • the device can be designed to save space, since no external drive is needed.
  • gassing or stirring elements can also be varied depending on the size of the reactor.
  • the inserts in the gassing elements could also be oriented obliquely downwards or upwards or horizontally.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé et un dispositif de gazage, en particulier pour la fermentation microbiologique et la culture de cellules dans un espace fermé. Il est prévu un réacteur stérilisable (1), un axe d'agitation vertical (2) sur lequel sont disposés un certain nombre d'éléments de gazage et/ou d'agitation (10) à plusieurs niveaux et superposés. Les éléments de gazage (15) sont constitués de telle sorte qu'ils entraînent ce gazage du liquide dans le réacteur de telle sorte que les gaz soient intégrés uniformément dans le liquide sous forme de micro-bulles. Pour la propulsion des éléments de gazage et d'agitation (10) aménagés sur l'axe d'agitation (2) monté de façon à pouvoir tourner (2), des tuyères à réaction (20) raccordées aux canaux d'apport de gaz (11, 12, 14) sont présentes. Ceci donne une disposition permettant d'économiser de la place et absolument dense de ce dispositif.
EP07819323A 2006-10-25 2007-10-25 Procédé et dispositif de gazage, en particulier pour la fermentation microbiologique et la culture de cellules Withdrawn EP2084260A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH01702/06A CH698723B1 (de) 2006-10-25 2006-10-25 Vorrichtung zum Begasen, insbesondere für die mikrobiologische Fermentation und Kultivierung von Zellen.
PCT/EP2007/009274 WO2008049617A1 (fr) 2006-10-25 2007-10-25 Procédé et dispositif de gazage, en particulier pour la fermentation microbiologique et la culture de cellules

Publications (1)

Publication Number Publication Date
EP2084260A1 true EP2084260A1 (fr) 2009-08-05

Family

ID=38921720

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07819323A Withdrawn EP2084260A1 (fr) 2006-10-25 2007-10-25 Procédé et dispositif de gazage, en particulier pour la fermentation microbiologique et la culture de cellules

Country Status (5)

Country Link
US (1) US9399203B2 (fr)
EP (1) EP2084260A1 (fr)
CN (1) CN101573436B (fr)
CH (1) CH698723B1 (fr)
WO (1) WO2008049617A1 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102233245B (zh) * 2011-07-13 2013-03-27 浙江长江搅拌设备有限公司 高效柔性结构气、固、液搅拌机
US9700857B1 (en) * 2012-03-23 2017-07-11 Life Technologies Corporation Fluid mixing system with drive shaft steady support
CN103045472A (zh) * 2013-01-25 2013-04-17 李明忠 生物发酵用气动搅拌机
CN105664819A (zh) * 2014-11-14 2016-06-15 程叶红 生物表活剂微乳液生产用微乳状液反应釜
CN105642213A (zh) * 2014-11-14 2016-06-08 程叶红 一种化工生物表活剂微乳液生产用微乳状液反应釜
WO2016092073A1 (fr) * 2014-12-12 2016-06-16 Universitat Autonoma De Barcelona Systèmes accouplés d'aération, d'agitation et d'échange de chaleur permettant la culture de micro-organismes dans des bioréacteurs à usage unique
EP3031895A1 (fr) * 2014-12-12 2016-06-15 Universitat Autònoma de Barcelona Système d'agitation et d'aération pour la culture de micro-organismes dans des bioréacteurs à usage unique
US10519409B2 (en) * 2014-12-31 2019-12-31 Ge Healthcare Bio-Sciences Corp. Shaft-mounted fluid transfer assembly for a disposable bioreactor
CN106076161A (zh) * 2016-04-28 2016-11-09 柳江县科益搪瓷有限公司 用于食用菌培养料的搅拌装置
CN109310958B (zh) * 2016-06-15 2021-12-14 安斋聪 养殖用或排水处理用超微细气泡产生装置
CN106902689B (zh) * 2017-04-28 2023-08-22 山东金祥云贸易有限公司 一种制砖混料装置
WO2018209133A1 (fr) * 2017-05-12 2018-11-15 Jerry Shevitz Bioréacteurs
CN108913595B (zh) * 2018-08-03 2023-10-03 滕海峰 一种植物体供能装置
CN109182098A (zh) * 2018-10-10 2019-01-11 南京和润隆环保科技有限公司 一种具有旋转搅拌管道的沼气池
CN109439516A (zh) * 2018-12-20 2019-03-08 巫溪县清味鲜食品有限公司 一种能制作醪糟的全自动电饭锅
CN110042046B (zh) * 2019-05-07 2022-10-04 南京工业大学 连续固定化的内置搅拌微膜曝气内循环气升式生物反应器
CN111826268A (zh) * 2020-07-22 2020-10-27 湖南广盛源医药科技有限公司 一种用于医用酒精生产的发酵装置
CN116120103B (zh) * 2023-03-23 2023-06-23 浙江大学 一种用于垃圾发酵的促曝气供氧装置及方法

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CH663422A5 (de) * 1982-05-27 1987-12-15 Chemap Ag Verfahren und fermenter zur zuechtung von gewebezellen.
US4836826A (en) * 1987-12-18 1989-06-06 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Magnetic drive coupling
CH677496A5 (fr) * 1988-04-05 1991-05-31 Gerd Kloss
US5779996A (en) * 1995-04-21 1998-07-14 Innovative Biosystems, Inc. Microbial remediation reactor and process
FR2784311B1 (fr) * 1998-10-09 2000-12-08 Air Liquide Dispositif d'agitation d'un liquide dans un reacteur et d'injection d'un gaz dans ce liquide
CH696494A5 (de) * 2003-04-30 2007-07-13 Andreas Kloss Vorrichtung zur mikrobiologischen Fermentation und Kultivierung von Zellen.
EP1473358A3 (fr) 2003-04-30 2005-06-08 Chemie- Und Tankanlagenbau Reuther Gmbh Procédé et dispositif pour le gazage et l'agitation de matières
DE102004062703A1 (de) * 2004-12-21 2006-06-22 Henkel Kgaa Mischbehälter mit Stromstörern
WO2007038893A1 (fr) * 2005-10-03 2007-04-12 Bruendler Ag Contenant jetable a agitateur

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Also Published As

Publication number Publication date
CN101573436A (zh) 2009-11-04
CN101573436B (zh) 2012-12-19
WO2008049617A1 (fr) 2008-05-02
US20100178685A1 (en) 2010-07-15
US9399203B2 (en) 2016-07-26
CH698723B1 (de) 2009-10-15

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