EP2010890A2 - Procede et systeme pour generer un resultat de mesure indiquant la presence d'une substance dans un echantillon sur la base d'une mesure spectrometrique - Google Patents

Procede et systeme pour generer un resultat de mesure indiquant la presence d'une substance dans un echantillon sur la base d'une mesure spectrometrique

Info

Publication number
EP2010890A2
EP2010890A2 EP07702562A EP07702562A EP2010890A2 EP 2010890 A2 EP2010890 A2 EP 2010890A2 EP 07702562 A EP07702562 A EP 07702562A EP 07702562 A EP07702562 A EP 07702562A EP 2010890 A2 EP2010890 A2 EP 2010890A2
Authority
EP
European Patent Office
Prior art keywords
intensity distribution
spectral intensity
light
measurement
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07702562A
Other languages
German (de)
English (en)
Inventor
Winfried Willemer
Jürgen KRIEG
Carl Sandhagen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
OPSOLUTION SPECTROSCOPIC SYSTEMS GmbH
Biozoom Technologies Inc
Original Assignee
Biozoom Services GmbH
OPSOLUTION SPECTROSCOPIC SYSTE
Opsolution Spectroscopic Systems GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biozoom Services GmbH, OPSOLUTION SPECTROSCOPIC SYSTE, Opsolution Spectroscopic Systems GmbH filed Critical Biozoom Services GmbH
Publication of EP2010890A2 publication Critical patent/EP2010890A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
    • G01N2021/3144—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths for oxymetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
    • G01N2021/3181—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths using LEDs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/121—Correction signals
    • G01N2201/1211—Correction signals for temperature

Definitions

  • the invention is directed to a method and apparatus for generating a measurement result indicative of the presence or concentration of a substance within a sample based on a spectrometric measurement, wherein light is emitted from a source to that sample as part of an exposure phase, and in interaction with This sample is recorded with respect to the spectral intensity distribution of this sample, and the presence of a substance is determined in the context of electronic measurement signal processing, including the spectral intensity distribution of the detected light.
  • the signal of the sample is promptly (relative to the drift of the apparatus) obtained signal of a blank or white light reference divided.
  • the intensity and the spectral distribution of the source must be identical in the above method when taking the signal of the sample and when receiving the signal of the zero sample. Deviation immediately leads to an error in determining the properties of the sample.
  • the simultaneous, mutual e.g. also partial recording of the signal of the sample and the blank sample allows the use of sources that need only be stable for the duration of a change cycle.
  • the intensity signal obtained at the same time by means of a non-spectrally sensitive detector can be used to compensate for the intensity fluctuations of the source, provided the spectral characteristics of the source and the detector or the system of source and Detector, for example, by a timely recording of the zero sample signal known and stable.
  • the invention has for its object to provide solutions by which it is possible to allow a reliable determination of the concentration of a substance in a spectrometric manner, the corresponding measurement without recording a reference measurement in close proximity to the measurement and without a preliminary phase to stabilize the Device can be handled within a relatively short time.
  • This object is according to a first aspect of the invention solved by a method for generating a measurement result indicative of the presence of a substance within a sample on the basis of a spectrometric measurement, in which
  • a compensation function is processed, which takes into account dependencies of the spectral intensity distribution of the light emitted by the light source of the temperature of the LED.
  • the measurement can be carried out directly with the ambient tempered device.
  • the indicative of the spectral intensity distribution of the light emitted by the light source indicative data can be generated in an advantageous manner within a calibration step.
  • a reference measurement is performed on a substantially known object (white reference).
  • the measurement result is canceled in any form.
  • the term "G *" is a unique function, it can be obtained from the difference of the logarithms of two reference measurements and then only has to be scaled or shifted in wavelength depending on the specific temperature differences.
  • Fig.l Measurements of two temperatures in digital units. From these curves, a compensation function is generated.
  • Fig.3 The difference between the curves in Figure 2. This curve can be used as a compensation function.
  • the pattern to be recognized is an artifact of the directional dependence of the spectral characteristic of the LED used. This can be eliminated by appropriate measures on the illumination optics used.
  • the compensation function can be adjusted to a temperature offset determined therefrom on the basis of the correlation of the spectral intensity distribution of the light emitted by the light source with the spectral intensity distribution of the detected light.
  • the compensation function can also be further tuned by additional auxiliary parameters. These auxiliary parameters can be determined from a correlation of the measured values with reference values. It is also possible to carry out further adjustments, in particular scaling of the data for the spectral intensity distribution of the light emitted by the light source and / or the spectral intensity distribution of the detected light.
  • the task may also be: search the parameters of the model functions so that the entire modal function reproduces the measured data as well as possible.
  • the model functions are given by the absorptions of the substances of the sample and - the clou '- the compensation function function.
  • the parameters are the concentrations and scaling of the shift of the compensation function function with respect to the wavelength.
  • an arbitrarily complex mapping (more than scaling and shifting) of the compensation function function to the concrete compensation function function can also exist; then the elements describing this figure were parameters to be determined.
  • the "correct model” and the measurement data correlate better with each other than a “wrong model” and the measurement data.
  • the LED is operated with a defined flow stream.
  • the LED can be operated under a compensated by the compensation function temperature increase.
  • the LED structure can be operated with connection to a heat sink.
  • the parameters belonging to a uniform compensation function simultaneously with the determination of the parameters
  • the substance-specific absorption can be determined in the context of a mathematical adaptation and in this way to compensate for the influence of the temperature. This is basically the core of the process.
  • the determination of the substance-specific absorptions can furthermore be carried out by determining, within predetermined temperature ranges, the parameters belonging to a uniform compensation function using a possible given mathematical independence sequentially for the determination of the parameters of the substance-specific absorption and in this way compensating the influence of the temperature.
  • LED light-emitting diodes
  • a spectrometer Using light-emitting diodes (LED) and a spectrometer, the influence of various substances in human tissue such as. Hemoglobin on the absorption wavelength dependent to be examined to allow statements about concentrations of the substances in question in the tissue to be examined.
  • the erfmdungsgeschreib working measuring device can be used without explicit reference measurement and without any special waiting time before the actual measurement.
  • the absorption can be measured directly by determining the decadic logarithm ⁇ of the ratio of the energies of the light emitted by the source in the relevant wavelength interval (or frequency interval) to those detected on a sensor in the relevant wavelength interval, in the sequence as optical density (OD); the absorption, which is due to the presence is caused by the difference in optical density in the presence and absence of the substance in question.
  • the spectrally resolved measurement makes it possible to deduce the absorption caused by certain substances by determining the wavelength-dependent course of the optical density, even if the optical density is known only up to a wavelength-independent constant (offset).
  • the absorptions caused by the respective substances can be determined.
  • Necessary condition is that the spectral distribution of the energy of the light source is identical in measurement and underlying.
  • the LEDs are mounted in such a way that the heating of the LED during a measurement can be neglected due to a particularly good heat dissipation, so that substantially only the ambient conditions have an influence on the temperature of the active layer of the LED.
  • the LED property demanded and used according to the invention is that the difference of the logarithms of the spectral intensity distributions at any temperature within a permissible range of application and the temperature at which a reference measurement is carried out can be represented in a sufficiently good approximation with respect to the measurement application a compensation function in conjunction with a simple illustration.
  • the LEDs are selected so that the said compensation function is determined by the difference of the logarithms of the spectral intensity distribution. or by the spectral distribution of the differences of the logarithms of each wavelength interval to the spectral intensity proportional magnitudes, which are recorded at two different suitable temperatures and that said simple mapping consists of a scaling and a displacement m of the wavelength.
  • the spectral intensity is related to frequency intervals and the compensation function is printed as a function of frequency.
  • the determination of the absorptions caused by the substances of interest from a measurement with a suitable device by a good adaptation to the falsified due to the temperature difference between a single reference measurement and actual measurement optical density from the relevant absorption functions of the functions of interest and the compensation function in combination with the associated figure is searched.
  • the parameters that uniquely determine the figure are free parameters of the adaptation.
  • the figure consists of a scaling and shift in the wavelength: here both the shift of the compensation function with respect to the wavelength and the scaling factor of the compensation function are free parameters of the adaptation.
  • a selection of the LED can be such that the temperature-dependent change in the spectral intensity in the spectral range to be considered is as small as possible. Furthermore, a selection of the LED, such that the associated compensation function in the spectral range under consideration with respect to the determination of the parameters of all functions to be adapted as independent as possible of the absorption functions of the substances of interest, ie, that just adapting only the compensation function to the measured optical density almost correct parameters with respect to displacement and scaling results, be advantageous.

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Engineering & Computer Science (AREA)
  • Mathematical Physics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Spectrometry And Color Measurement (AREA)

Abstract

L'invention concerne un procédé et un dispositif pour générer un résultat de mesure indiquant la présence ou la concentration d'une substance dans un échantillon. L'invention vise à créer des solutions qui permettent de déterminer de manière fiable la concentration d'une substance par spectrométrie, la mesure correspondante pouvant être réalisée en un temps relativement court, sans enregistrement d'une mesure de référence faite à proximité temporelle directe de la mesure et sans phase préalable nécessaire à la stabilisation de l'appareil. Dans un premier mode de réalisation, selon un procédé permettant de générer un résultat de mesure indiquant la présence d'une substance dans un échantillon sur la base d'une mesure spectrométrique, une lumière émise par une source est dirigée sur l'échantillon lors d'une phase d'éclairage et la répartition d'intensité spectrale de la lumière imprimée en interaction avec l'échantillon est saisie. Dans le cadre d'un traitement mathématique assisté par ordinateur de signaux de mesure, la répartition d'intensité spectrale de la lumière saisie étant prise en considération, la concentration d'une substance ou une grandeur dépendant ce cette concentration est déterminée, la source de lumière étant une DEL. Dans le cadre d'un traitement mathématique assisté par ordinateur de signaux de mesure, une fonction de compensation est élaborée, cette fonction tenant compte de la dépendance de la répartition d'intensité spectrale de la lumière émise par la source lumineuse par rapport à la température de la DEL. Il est ainsi avantageusement possible de réaliser une mesure spectrométrique sans phase de mise en température du système de mesure, phase qui prend du temps, la mesure pouvant être réalisée directement au moyen de l'appareil à température ambiante.
EP07702562A 2005-12-30 2007-01-02 Procede et systeme pour generer un resultat de mesure indiquant la presence d'une substance dans un echantillon sur la base d'une mesure spectrometrique Withdrawn EP2010890A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200510063263 DE102005063263A1 (de) 2005-12-30 2005-12-30 Verfahren und System zur Generierung eines hinsichtlich der Präsenz eines Stoffes innerhalb einer Probe indikativen Messergebnisses auf Grundlage einer spektrometrischen Messung
PCT/EP2007/000012 WO2007077208A2 (fr) 2005-12-30 2007-01-02 Procede et systeme pour generer un resultat de mesure indiquant la presence d'une substance dans un echantillon sur la base d'une mesure spectrometrique

Publications (1)

Publication Number Publication Date
EP2010890A2 true EP2010890A2 (fr) 2009-01-07

Family

ID=38135799

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07702562A Withdrawn EP2010890A2 (fr) 2005-12-30 2007-01-02 Procede et systeme pour generer un resultat de mesure indiquant la presence d'une substance dans un echantillon sur la base d'une mesure spectrometrique

Country Status (3)

Country Link
EP (1) EP2010890A2 (fr)
DE (1) DE102005063263A1 (fr)
WO (1) WO2007077208A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2010875A1 (fr) * 2006-04-19 2009-01-07 Opsolution Spectroscopic Systems GmbH Procédé pour compenser des variations de température de source lumineuse dans des spectromètres
EP2218472B2 (fr) * 2009-02-11 2022-03-16 B. Braun Avitum AG Dispositif destiné au traitement du sang extracorporel
CN101806743B (zh) * 2010-03-16 2012-10-31 李华 便携式广谱分光光度液体微量元素检测仪及检测方法
EP2511693A1 (fr) * 2011-04-13 2012-10-17 F. Hoffmann-La Roche AG Système d'analyse avec source lumineuse contrôlée spectralement
DE102017108552B4 (de) 2017-04-21 2018-11-15 ams Sensors Germany GmbH Spektrometrischer Messkopf mit mehreren Transmissionslicht-Eintrittsfenstern

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5477853A (en) * 1992-12-01 1995-12-26 Somanetics Corporation Temperature compensation method and apparatus for spectroscopic devices
GB0322545D0 (en) * 2003-09-26 2003-10-29 Whitland Res Ltd Measurement of blood oxygen saturation (SO2)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2007077208A3 *

Also Published As

Publication number Publication date
WO2007077208A2 (fr) 2007-07-12
DE102005063263A1 (de) 2007-07-05
WO2007077208A3 (fr) 2008-01-10

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