EP1984504A2 - Production de proteines porteuses d'oligomannose ou de glycanes de type humain dans des levures, et leurs procedes d' utilisation - Google Patents
Production de proteines porteuses d'oligomannose ou de glycanes de type humain dans des levures, et leurs procedes d' utilisationInfo
- Publication number
- EP1984504A2 EP1984504A2 EP07717023A EP07717023A EP1984504A2 EP 1984504 A2 EP1984504 A2 EP 1984504A2 EP 07717023 A EP07717023 A EP 07717023A EP 07717023 A EP07717023 A EP 07717023A EP 1984504 A2 EP1984504 A2 EP 1984504A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- polypeptide
- pichia
- cell
- mucin
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4727—Mucins, e.g. human intestinal mucin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne le développement de lignées cellulaires ayant des voies de glycosylation modifiées qui leur permettent de réaliser une séquence de réactions enzymatiques, qui imitent le traitement de glycoprotéines chez les êtres humains. Des protéines recombinantes exprimées dans ces hôtes synthétisés par génie génétique produisent des glycoprotéines davantage similaires, voire sensiblement identiques, à leurs homologues humains. Les eucaryotes inférieurs, qui produisent ordinairement des N-glycanes à teneur élevée en mannose, comprenant des champignons unicellulaires et multicellulaires, sont modifiés de façon à produire des O-glycanes ou autres structures parmi les voies de glycosylation humaine. Ceci est obtenu à l'aide d'une combinaison de synthèse par génie génétique et/ou sélection de souches qui n'expriment pas certaines enzymes créant les structures complexes indésirables caractéristiques des glycoprotéines fongiques, qui expriment des cellules exogènes sélectionnées soit pour avoir une activité optimale dans les conditions présentes dans les champignons où l'activité est désirée, soit qui sont dirigées vers un organelle où une activité optimale est obtenue, et des combinaisons de ceux-ci dans lesquelles l'eucaryote synthétisé par génie génétique exprime de multiples enzymes exogènes requises afin de produire des glycoprotéines “de type humain”.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US76163206P | 2006-01-23 | 2006-01-23 | |
US2007002094 | 2007-01-23 |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1984504A2 true EP1984504A2 (fr) | 2008-10-29 |
Family
ID=46045587
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP07717023A Pending EP1984504A2 (fr) | 2006-01-23 | 2007-01-23 | Production de proteines porteuses d'oligomannose ou de glycanes de type humain dans des levures, et leurs procedes d' utilisation |
Country Status (1)
Country | Link |
---|---|
EP (1) | EP1984504A2 (fr) |
-
2007
- 2007-01-23 EP EP07717023A patent/EP1984504A2/fr active Pending
Non-Patent Citations (1)
Title |
---|
See references of WO2007087420A3 * |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20080821 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR |
|
AX | Request for extension of the european patent |
Extension state: AL BA HR MK RS |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: C07K 14/47 20060101AFI20081107BHEP |
|
D17P | Request for examination filed (deleted) |